LOCUS       DAZKOK010000001        84952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000001 DAZKOK010000000
VERSION     DAZKOK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.001"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..523
                     /locus_tag="ACG90W_000001"
     CDS             5..523
                     /locus_tag="ACG90W_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0476400.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            651..1334
                     /locus_tag="ACG90W_000002"
     CDS             651..1334
                     /locus_tag="ACG90W_000002"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0476401.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1643..1716
                     /locus_tag="ACG90W_000003"
     tRNA            1643..1716
                     /locus_tag="ACG90W_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1677..1679,aa:Arg,seq:tct)
     gene            1875..2234
                     /locus_tag="ACG90W_000004"
     CDS             1875..2234
                     /locus_tag="ACG90W_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0476402.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2357..3988
                     /locus_tag="ACG90W_000005"
     CDS             2357..3988
                     /locus_tag="ACG90W_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0476403.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4050..4691)
                     /locus_tag="ACG90W_000006"
     CDS             complement(4050..4691)
                     /locus_tag="ACG90W_000006"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0476404.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4877..4953
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4973..5281
                     /gene="rplU"
                     /locus_tag="ACG90W_000007"
     CDS             4973..5281
                     /gene="rplU"
                     /locus_tag="ACG90W_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0476405.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5308..5652
                     /locus_tag="ACG90W_000008"
     CDS             5308..5652
                     /locus_tag="ACG90W_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0476406.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5665..5958
                     /gene="rpmA"
                     /locus_tag="ACG90W_000009"
     CDS             5665..5958
                     /gene="rpmA"
                     /locus_tag="ACG90W_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0476407.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6392..9199
                     /locus_tag="ACG90W_000010"
     CDS             6392..9199
                     /locus_tag="ACG90W_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0476408.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9391..9882
                     /locus_tag="ACG90W_000011"
     CDS             9391..9882
                     /locus_tag="ACG90W_000011"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0476409.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9985..10974
                     /locus_tag="ACG90W_000012"
     CDS             9985..10974
                     /locus_tag="ACG90W_000012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0476410.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11007..11810
                     /locus_tag="ACG90W_000013"
     CDS             11007..11810
                     /locus_tag="ACG90W_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0476411.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11831..12745
                     /locus_tag="ACG90W_000014"
     CDS             11831..12745
                     /locus_tag="ACG90W_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0476412.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12828..13202
                     /locus_tag="ACG90W_000015"
     CDS             12828..13202
                     /locus_tag="ACG90W_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0476413.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13341..13586
                     /locus_tag="ACG90W_000016"
     CDS             13341..13586
                     /locus_tag="ACG90W_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0476414.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13758..14822
                     /locus_tag="ACG90W_000017"
     CDS             13758..14822
                     /locus_tag="ACG90W_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0476415.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15068..15493
                     /locus_tag="ACG90W_000018"
     CDS             15068..15493
                     /locus_tag="ACG90W_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0476416.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15486..15935
                     /gene="nusB"
                     /locus_tag="ACG90W_000019"
     CDS             15486..15935
                     /gene="nusB"
                     /locus_tag="ACG90W_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0476417.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16032..16886
                     /locus_tag="ACG90W_000020"
     CDS             16032..16886
                     /locus_tag="ACG90W_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0476418.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16887..18224
                     /gene="xseA"
                     /locus_tag="ACG90W_000021"
     CDS             16887..18224
                     /gene="xseA"
                     /locus_tag="ACG90W_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0476419.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18229..18453
                     /locus_tag="ACG90W_000022"
     CDS             18229..18453
                     /locus_tag="ACG90W_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0476420.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18455..19345
                     /locus_tag="ACG90W_000023"
     CDS             18455..19345
                     /locus_tag="ACG90W_000023"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0476421.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19354..20172
                     /locus_tag="ACG90W_000024"
     CDS             19354..20172
                     /locus_tag="ACG90W_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0476422.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20279..20728
                     /locus_tag="ACG90W_000025"
     CDS             20279..20728
                     /locus_tag="ACG90W_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0476423.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20746..22428
                     /gene="recN"
                     /locus_tag="ACG90W_000026"
     CDS             20746..22428
                     /gene="recN"
                     /locus_tag="ACG90W_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0476424.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22483..22599)
                     /locus_tag="ACG90W_000027"
     CDS             complement(22483..22599)
                     /locus_tag="ACG90W_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0476425.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22672..23628)
                     /locus_tag="ACG90W_000028"
     CDS             complement(22672..23628)
                     /locus_tag="ACG90W_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0476426.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23799..24167
                     /locus_tag="ACG90W_000029"
     CDS             23799..24167
                     /locus_tag="ACG90W_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0476427.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24337..24768
                     /gene="mraZ"
                     /locus_tag="ACG90W_000030"
     CDS             24337..24768
                     /gene="mraZ"
                     /locus_tag="ACG90W_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0476428.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24789..25748
                     /gene="rsmH"
                     /locus_tag="ACG90W_000031"
     CDS             24789..25748
                     /gene="rsmH"
                     /locus_tag="ACG90W_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0476429.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25753..26154
                     /gene="ftsL"
                     /locus_tag="ACG90W_000032"
     CDS             25753..26154
                     /gene="ftsL"
                     /locus_tag="ACG90W_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0476430.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26155..28347
                     /locus_tag="ACG90W_000033"
     CDS             26155..28347
                     /locus_tag="ACG90W_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0476431.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28373..29335
                     /gene="mraY"
                     /locus_tag="ACG90W_000034"
     CDS             28373..29335
                     /gene="mraY"
                     /locus_tag="ACG90W_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0476432.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29349..30710
                     /gene="murD"
                     /locus_tag="ACG90W_000035"
     CDS             29349..30710
                     /gene="murD"
                     /locus_tag="ACG90W_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0476433.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30736..31824
                     /gene="murG"
                     /locus_tag="ACG90W_000036"
     CDS             30736..31824
                     /gene="murG"
                     /locus_tag="ACG90W_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0476434.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31839..33059
                     /locus_tag="ACG90W_000037"
     CDS             31839..33059
                     /locus_tag="ACG90W_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0476435.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33215..34540
                     /gene="ftsA"
                     /locus_tag="ACG90W_000038"
     CDS             33215..34540
                     /gene="ftsA"
                     /locus_tag="ACG90W_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0476436.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34563..35804
                     /gene="ftsZ"
                     /locus_tag="ACG90W_000039"
     CDS             34563..35804
                     /gene="ftsZ"
                     /locus_tag="ACG90W_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0476437.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35821..36498
                     /locus_tag="ACG90W_000040"
     CDS             35821..36498
                     /locus_tag="ACG90W_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0476438.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36512..37111
                     /locus_tag="ACG90W_000041"
     CDS             36512..37111
                     /locus_tag="ACG90W_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0476439.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37192..37413
                     /locus_tag="ACG90W_000042"
     CDS             37192..37413
                     /locus_tag="ACG90W_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0476440.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37462..38244
                     /locus_tag="ACG90W_000043"
     CDS             37462..38244
                     /locus_tag="ACG90W_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0476441.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38265..38972
                     /locus_tag="ACG90W_000044"
     CDS             38265..38972
                     /locus_tag="ACG90W_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0476442.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39234..42032
                     /gene="ileS"
                     /locus_tag="ACG90W_000045"
     CDS             39234..42032
                     /gene="ileS"
                     /locus_tag="ACG90W_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0476443.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42081..43607)
                     /gene="zwf"
                     /locus_tag="ACG90W_000046"
     CDS             complement(42081..43607)
                     /gene="zwf"
                     /locus_tag="ACG90W_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0476444.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43622..44269)
                     /locus_tag="ACG90W_000047"
     CDS             complement(43622..44269)
                     /locus_tag="ACG90W_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0476445.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44453..44782)
                     /locus_tag="ACG90W_000048"
     CDS             complement(44453..44782)
                     /locus_tag="ACG90W_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0476446.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44959..45687
                     /gene="sfsA"
                     /locus_tag="ACG90W_000049"
     CDS             44959..45687
                     /gene="sfsA"
                     /locus_tag="ACG90W_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0476447.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45703..46716
                     /locus_tag="ACG90W_000050"
     CDS             45703..46716
                     /locus_tag="ACG90W_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0476448.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46716..47993
                     /locus_tag="ACG90W_000051"
     CDS             46716..47993
                     /locus_tag="ACG90W_000051"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0476449.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48056..50758
                     /locus_tag="ACG90W_000052"
     CDS             48056..50758
                     /locus_tag="ACG90W_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0476450.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50910..51227
                     /locus_tag="ACG90W_000053"
     CDS             50910..51227
                     /locus_tag="ACG90W_000053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0476451.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51257..51577
                     /locus_tag="ACG90W_000054"
     CDS             51257..51577
                     /locus_tag="ACG90W_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0476452.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51685..53145
                     /locus_tag="ACG90W_000055"
     CDS             51685..53145
                     /locus_tag="ACG90W_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0476453.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53213..53434
                     /locus_tag="ACG90W_000056"
     CDS             53213..53434
                     /locus_tag="ACG90W_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476454.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53465..53647
                     /locus_tag="ACG90W_000057"
     CDS             53465..53647
                     /locus_tag="ACG90W_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0476455.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53647..54060
                     /locus_tag="ACG90W_000058"
     CDS             53647..54060
                     /locus_tag="ACG90W_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0476456.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54183..55364
                     /locus_tag="ACG90W_000059"
     CDS             54183..55364
                     /locus_tag="ACG90W_000059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0476457.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55435..55599)
                     /locus_tag="ACG90W_000060"
     CDS             complement(55435..55599)
                     /locus_tag="ACG90W_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476458.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55753..55826
                     /locus_tag="ACG90W_000061"
     tRNA            55753..55826
                     /locus_tag="ACG90W_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55787..55789,aa:Arg,seq:cct)
     gene            complement(55895..57034)
                     /locus_tag="ACG90W_000062"
     CDS             complement(55895..57034)
                     /locus_tag="ACG90W_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0476459.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57206..57340
                     /locus_tag="ACG90W_000063"
     CDS             57206..57340
                     /locus_tag="ACG90W_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476460.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57333..57968)
                     /locus_tag="ACG90W_000064"
     CDS             complement(57333..57968)
                     /locus_tag="ACG90W_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476461.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58081..58716)
                     /locus_tag="ACG90W_000065"
     CDS             complement(58081..58716)
                     /locus_tag="ACG90W_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0476462.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58750..59211)
                     /locus_tag="ACG90W_000066"
     CDS             complement(58750..59211)
                     /locus_tag="ACG90W_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0476463.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59341..59772)
                     /locus_tag="ACG90W_000067"
     CDS             complement(59341..59772)
                     /locus_tag="ACG90W_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0476464.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59790..60110)
                     /locus_tag="ACG90W_000068"
     CDS             complement(59790..60110)
                     /locus_tag="ACG90W_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0476465.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60409..61185
                     /locus_tag="ACG90W_000069"
     CDS             60409..61185
                     /locus_tag="ACG90W_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0476466.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61200..61403
                     /locus_tag="ACG90W_000070"
     CDS             61200..61403
                     /locus_tag="ACG90W_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0476467.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61419..61757
                     /locus_tag="ACG90W_000071"
     CDS             61419..61757
                     /locus_tag="ACG90W_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0476468.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61744..61923
                     /locus_tag="ACG90W_000072"
     CDS             61744..61923
                     /locus_tag="ACG90W_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476469.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(61966..62436)
                     /locus_tag="ACG90W_000073"
     CDS             complement(61966..62436)
                     /locus_tag="ACG90W_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476470.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62523..63221
                     /locus_tag="ACG90W_000074"
     CDS             62523..63221
                     /locus_tag="ACG90W_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0476471.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63399..63740
                     /locus_tag="ACG90W_000075"
     CDS             63399..63740
                     /locus_tag="ACG90W_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476472.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63733..64404
                     /locus_tag="ACG90W_000076"
     CDS             63733..64404
                     /locus_tag="ACG90W_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0476473.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64410..65102
                     /locus_tag="ACG90W_000077"
     CDS             64410..65102
                     /locus_tag="ACG90W_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0476474.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            65099..65848
                     /locus_tag="ACG90W_000078"
     CDS             65099..65848
                     /locus_tag="ACG90W_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0476475.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65860..66129
                     /locus_tag="ACG90W_000079"
     CDS             65860..66129
                     /locus_tag="ACG90W_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476476.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            66135..66299
                     /locus_tag="ACG90W_000080"
     CDS             66135..66299
                     /locus_tag="ACG90W_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476477.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66292..66591
                     /locus_tag="ACG90W_000081"
     CDS             66292..66591
                     /locus_tag="ACG90W_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476478.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            66591..66893
                     /locus_tag="ACG90W_000082"
     CDS             66591..66893
                     /locus_tag="ACG90W_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0476479.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            66933..67298
                     /locus_tag="ACG90W_000083"
     CDS             66933..67298
                     /locus_tag="ACG90W_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476480.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            67788..67928
                     /locus_tag="ACG90W_000084"
     CDS             67788..67928
                     /locus_tag="ACG90W_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476481.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            67925..68107
                     /locus_tag="ACG90W_000085"
     CDS             67925..68107
                     /locus_tag="ACG90W_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476482.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            68120..68314
                     /locus_tag="ACG90W_000086"
     CDS             68120..68314
                     /locus_tag="ACG90W_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476483.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(68347..68559)
                     /locus_tag="ACG90W_000087"
     CDS             complement(68347..68559)
                     /locus_tag="ACG90W_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476484.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            68687..68887
                     /locus_tag="ACG90W_000088"
     CDS             68687..68887
                     /locus_tag="ACG90W_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476485.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            68884..69156
                     /locus_tag="ACG90W_000089"
     CDS             68884..69156
                     /locus_tag="ACG90W_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0476486.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            69157..69342
                     /locus_tag="ACG90W_000090"
     CDS             69157..69342
                     /locus_tag="ACG90W_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476487.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            69320..69676
                     /locus_tag="ACG90W_000091"
     CDS             69320..69676
                     /locus_tag="ACG90W_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476488.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            69636..70112
                     /locus_tag="ACG90W_000092"
     CDS             69636..70112
                     /locus_tag="ACG90W_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0476489.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(70258..70986)
                     /locus_tag="ACG90W_000093"
     CDS             complement(70258..70986)
                     /locus_tag="ACG90W_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0476490.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            71034..71312
                     /locus_tag="ACG90W_000094"
     CDS             71034..71312
                     /locus_tag="ACG90W_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476491.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            71314..71700
                     /locus_tag="ACG90W_000095"
     CDS             71314..71700
                     /locus_tag="ACG90W_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0476492.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            71697..72077
                     /locus_tag="ACG90W_000096"
     CDS             71697..72077
                     /locus_tag="ACG90W_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0476493.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            72189..72641
                     /locus_tag="ACG90W_000097"
     CDS             72189..72641
                     /locus_tag="ACG90W_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0476494.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            72638..74365
                     /locus_tag="ACG90W_000098"
     CDS             72638..74365
                     /locus_tag="ACG90W_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0476495.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            74429..75616
                     /locus_tag="ACG90W_000099"
     CDS             74429..75616
                     /locus_tag="ACG90W_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0476496.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            75588..76157
                     /locus_tag="ACG90W_000100"
     CDS             75588..76157
                     /locus_tag="ACG90W_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0476497.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            76170..77534
                     /locus_tag="ACG90W_000101"
     CDS             76170..77534
                     /locus_tag="ACG90W_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0476498.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            77536..77817
                     /locus_tag="ACG90W_000102"
     CDS             77536..77817
                     /locus_tag="ACG90W_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0476499.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            77795..78121
                     /locus_tag="ACG90W_000103"
     CDS             77795..78121
                     /locus_tag="ACG90W_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0476500.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            78111..78449
                     /locus_tag="ACG90W_000104"
     CDS             78111..78449
                     /locus_tag="ACG90W_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476501.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            78439..78804
                     /locus_tag="ACG90W_000105"
     CDS             78439..78804
                     /locus_tag="ACG90W_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0476502.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            78811..79437
                     /locus_tag="ACG90W_000106"
     CDS             78811..79437
                     /locus_tag="ACG90W_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0476503.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            79437..79901
                     /locus_tag="ACG90W_000107"
     CDS             79437..79901
                     /locus_tag="ACG90W_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476504.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            80096..82405
                     /locus_tag="ACG90W_000108"
     CDS             80096..82405
                     /locus_tag="ACG90W_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0476505.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            82417..83121
                     /locus_tag="ACG90W_000109"
     CDS             82417..83121
                     /locus_tag="ACG90W_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0476506.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            83118..>84952
                     /locus_tag="ACG90W_000110"
     CDS             83118..>84952
                     /locus_tag="ACG90W_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0476507.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        28619 a        13994 c        17852 g        24487 t
ORIGIN      
        1 aacgatacct gattacaaaa aaataacttt tataagttct ataacgttga ttatattttt
       61 aattcttatt cttctcgtcg tcttggttcc ccgaattttt ggtttgactc cttatattat
      121 tgaggacaaa gaaatgagtc catattatgc acaaggaaat ctgatatatg tacgaccgaa
      181 aaatacaaat gagattttag tcggagatat tattacatat tatgaaaatt caggaaaaaa
      241 aatctcgacg cgtcgcgtaa tagccgttga aaacaaccac gaagattttt atacaaaagc
      301 tgatacaaag acttatattg agcctggagt agtaaaaaag agaaatatta tcggtagtcc
      361 catctttcag attccatata ttggactagt cctttcaaaa acggcatttg catggattca
      421 ggggttattc tttacgattg ctttttgcct aacaggaatc acagcatgga atacctttgt
      481 agagttgaaa aagaagcgga gacgaacaca gaaatttttg taagaatgtt aaaaacataa
      541 atgtattcca gagatccaac gactcttctg aacatgaaac acgagaaaac actcatgcag
      601 cttgtagatt tagtcagttg ttcgtataat gtgtgaagag gaggagtagt atgcgcaatg
      661 aaatgatgca tcgtcctgta gtcaaagaag aactattgga ttttatgcgt acccagcaaa
      721 aaaaattacc gggtgagcta ggcaaactgg aagaagaagc tcatgtagca gaagtaccag
      781 ttattcccca tgaaacagtt gtatttttga aatttttact tggacagctt cagcctgaac
      841 gaatcttgga gatcggagca gctatcggtt tttcatcaag tgtcatggcc acgacaatcg
      901 gagaaaacgc tcatgttacg acaattgatc gtttcgatgt catgatcgag aaagctaaaa
      961 aaaactatga acgtctagga ctgacagata aagtgacttt gcttgaaggt caagcagcag
     1021 atatcttgcc ggagttgtct ggtccgtatg actttatttt tatggatagt gcaaaatcaa
     1081 aatatattga atttttacct gaatgcttac gtttgttaag aaaaggtggc gttttgatgg
     1141 tagatgacat cttccaagga ggaacgatct tgctcccaga tgaagagatt ccccgtggca
     1201 aacgagctat ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat catcccgatt
     1261 tgacatcgac aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag gaatcagaaa
     1321 caatcgagtt gtgatacatg attatggcag cctgtagata atcgattgaa tcgagggctc
     1381 aattcgaact tgatgtaaaa gtttctatat ttgtaagtca attaaaggat acaggaaatc
     1441 taaataaaca ggagtagcat gaagtcaatg tagctcctgt tttcaattga taaccttaga
     1501 acagctgaag gagaatgtct cgttttaaaa gattgattta gtagttgggc caaaataatt
     1561 cattatttgc taattaaata actatttcgt attgacagaa gcaatttaat tatctataat
     1621 tattgatgtt ccgctgattc ttgtcatagt agctcagctg gatagagcat ccgccttcta
     1681 agcggacggt cgggggttcg aatccctcct gtgacgctaa aaaaccgtta gaaactgagg
     1741 tttctaacgg tttttctttg ttttttgtgc caatattgtt tgttttcaaa ggcaaataaa
     1801 ataaaaaaac atccttatcg agtacaatta taaggagcaa aaaaataaaa ctcatcagaa
     1861 aagaggaaga agatatgacg atactatcta gaattttagt gactctagtt gcaattgaat
     1921 ttttttatat catgtatatc gaaacggtga gaactgattc tgacacgaca agtagggttt
     1981 ttaaaatgag taaagaagag ttaagcagaa aatctgttca aacattgttt aagaaccaag
     2041 gagtgtacaa tggactatta ggagtgggac tactttatgg agcttatctt tcttccgcat
     2101 ctaaagaaat cacaagtatg ctattaatat ctattttttt tgttgcatta tatggcagtt
     2161 tgactagcga tcgttggatt ttactcaaac aaggtgggct agctctgttg gcattgcttt
     2221 ctattttctt ataaatatag aaaaaataag aatttatcta acaggaaact ttgtgaaaaa
     2281 tattacttta tctcatgaaa tatgatattc taatgaatgg tattttagaa aatttcatga
     2341 aaaaggagtt agataggtgt tcaaaaatta tgtttcctca ctaaaaaaag aattttcagg
     2401 ttatgatctt tcaagatttc agaaagatct tttggctggg atcacagtag ctgctgtagc
     2461 attgccttta gccttggctt ttggggtatc aagcggagct gatgcggccg caggtttgat
     2521 tacggctatc attgccggta tcgttattgg cggtttatca ggcggttttt atcaaatatc
     2581 aggtccgaca ggtgcgatgg cagctatctt gatgtctatt gcggcaacaa agggaatgca
     2641 aggtgtattg cttgctactt ttttagcggg tctattttta cttgcagcag gacttttccg
     2701 attaggaaca ctgacttctt ttatcccagc acccgtaatt acggggttta cttcaggtat
     2761 tgctatcatt attgcattag gtcagatcga taatttattt ggtgttcact cggaaggtac
     2821 aaacgttatt gaaaaactaa gtagttatca aacgatagga tttccgataa acacagcctc
     2881 tttgatcatg ggagggctag tcattttagg gatgtttatc tatccaaaaa aatgggcgaa
     2941 acgaatgcct agttcgctgc tggctatcat tttggcaaca gcagttatgc tattgttcca
     3001 tttaccagta gcaactgtgg gtaagattcc acaaacattg atcagtagta atcgtttgaa
     3061 tatgggagat tttagctttt ctgccttgca acaagtggca gttccagcta ttagcattgc
     3121 tttattagga atgattgaaa gcttgctatg tggtgcttcg gctggccgaa tggcaaataa
     3181 gcccttagat agcaatcaag aattaatggc gcaaggaatt ggaaatctgt tgcttccttt
     3241 ctttggcggg attcctgcta ctgcggccat cgcccgtaca agcgtggcca tcaaatctgg
     3301 tgcacaaact agattagctg gtatgataca tgcagttgtc ttactgctct ccatgcttgt
     3361 ttttgcgcca gtgatgtcac agattccaat gccagcactt gcaggtgttt tgattgtgac
     3421 ggcttggcgt atgaatgaat gggaaacgat caaggagcta tttactaata aatattggtc
     3481 agcagttcta ttgtttatcc ttacgatgat ttgtacagtt atctttgatt taagtattgc
     3541 tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt gtcaaaagcg ctgctatcac
     3601 gatttcatta gaaacgatcg attggcaaag aatggggctg ccagaaacgg aaaagctgaa
     3661 taactggaca gtagtgtata ttagtggtcc actgtttttt atgtcttcag aaaaactcaa
     3721 aaataaattg gaagaactaa atcatcacga tggtgtcatt ttctccatgc gaggcgtacc
     3781 aagtattgat ttgacggcac gtgcagtttt tgatgaattt caagaaaaag cagagcaaag
     3841 aggacaaacg attatctata cttccttgca gccagaagta gaaaaacaac tttctcactt
     3901 gtgggaacat acattcacgc atcaacatca aacagttgct catgcgttga cagctttaca
     3961 tgcgaaatat actgcaatta gccattaaaa caatgaaaaa gaacagacaa gactgtaaac
     4021 tgaagatttt atggtcttgt ctgttctttt taatattgct gtaaaagtga agctagtact
     4081 tgccagtctt gatggataac aggtacaagg gagcgattgt agaaaatagc tgctggatga
     4141 aaaagtggaa gaattcgata gtttttttct gaccattcgt aggcatcctt ttgttctgtt
     4201 aattgtaaga caggccctgt atagagttga ccatgatttt tggtaaccgt atattctttg
     4261 ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa ttaattttgg ctggaccttc
     4321 atcagttcgt aatccaatat tggtgcatgc gcaaatactt cggaacgagt aggtgtccga
     4381 tttggataaa cagtttctga ctgttgtgtc cgtttattga tccgatgagt cacgcgatac
     4441 gggcggctcc ttacagcact ggtgatatac acctcttctc gtgttaagcc agttgaagaa
     4501 agtgcctgca tcaattcttt tccagcttga ccgctaaaag gaacatgcgt atcaatctct
     4561 gttttccctg gggcttctcc cacgagcatc agtttaggat gaagaggtcc ttgcccagcg
     4621 ataaatcctt ctacaggacg gccgttcatt cttaaagaag cttcttgtat cagttctttt
     4681 gggtattcca tttatgctcc tcctttttct tttattataa gaataaaaga aacacaatgc
     4741 gactatttta tttggtagaa ctgtgaggat agaaaagcag aagtattgct tgaatttttg
     4801 ttttactttt tgattttaaa tttagttgac taacggttgc tgttctggta agctagtact
     4861 gttgtaattg tggactccac agctacaacc gctcagaaca agtaataagc acagcatatg
     4921 ctgtcacttc ggatggcgag tctaagtata tcagaaggag gtgctttata gcatgtacgc
     4981 aattattaaa actggtggta aacaagtaaa agttgaagtg ggacaagcaa tctatgttga
     5041 aaaacttgac gttgaagcag gagaaaaagt tgtttttgac gaagttatct tagtaggcgg
     5101 agaatctaca aaagtaggcg ctccaactgt tgcaggtgca actgttgaag gtactgttga
     5161 aaaacacggc aaacaaaaga aagtcgttac tttcaaatac aaacctaaaa aacattcaca
     5221 ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt atcgacgcga tcaacgctta
     5281 atagcgtttg agaaagaagg cctatatatg atcaaaggaa catttaagcg aaatgacgca
     5341 ggtcagatcg tttcattcac tttgaccggg catgctgatg ctggtcctta tggaagtgat
     5401 atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg tcaacgggat tgcctcatta
     5461 gccggttttg agccgattgt tgaaatgaat gaagaagaag gcggttatct ttataccgaa
     5521 gtcacttcag gaatgacaca agaacaaaac aatattgctg agattctatt agaaaatctt
     5581 ttattaggat tacaatcgat agaagctgaa aactcagaat atatccaaat taaaacaatt
     5641 aatgataaat aggaggtgca gaccatgttg ttatctatga atctacaatt atttgcccac
     5701 aaaaaaggtg gcggttctac atctaacggc cgtgactcag aatcaaaacg tctaggtgct
     5761 aaacgtgctg atggacaaac agttacaggt ggatcaattc tttaccgtca acgcggtaca
     5821 aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg acactttatt tgcaaaagtt
     5881 gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa aacaagtgtc tgtttatcca
     5941 gtagctcaag aagcttaatt gaatcttatg attaaagagg ttgtgacaag acttttgtca
     6001 cagccttttt tgtttataaa aataaaagtt gtagtggagg ctacgaagtt tactttgtct
     6061 ttttacactt aatatttttt tgccaatttg gaatcaggat gttttgataa ctatcgtttt
     6121 caattgatgg aagctatatt gtttgctatc agtgaaataa ttatatggta aacagtgttc
     6181 tttttcttgt ttccattata aaaatcgtat cgggattttt cgttctttta aattgaacat
     6241 ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa
     6301 ataaatacat agtattatgt tttgaagcct aagtacaata gtctatagaa aaggagcgat
     6361 accattggca aaacgcattg accgaattta tatgtatgtc aaagaaaaaa ccgctcattt
     6421 aacaaaggca gaatatgatc aaggagtaac aacacaggaa attgctgaag tgttagggat
     6481 tcagcgaaca aatagcagta aagatctgaa tcagctagtg cgcgaaggaa aactgataaa
     6541 gacagacgga cgaccagtac gttacatata tcaaacactc gttacgcacg taaagccagt
     6601 tacaaaacat gtagtcagtt acaaagaaac gccggtctct gaagaaaaaa atacactcca
     6661 aatagatact aaggatatct ttgcaaaaat cattggagct aacggcagca tgaaaaattc
     6721 agtggaacag gcaaaagctg cgattttgta tccgccaaga ggattaaatt gtttgattac
     6781 aggacctacc ggatcaggaa agacttattt tgcacacgcg atgtatcatt ttgcaaaagc
     6841 gcatcatgta atagatgagg aaacggaact gattgttttc aattgtgctg attatgccaa
     6901 taaccctgag cttttgatga gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc
     6961 agatgaagaa aaaatgggga ttatcgatca agcggatggc ggtatgctgt ttttggatga
     7021 gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt tactttatgg atcatggaac
     7081 ttacagtcgt cttggggaaa caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc
     7141 gaccacagaa gacccaggtt cttctctatt agaaactttt gttcggcgta tcccaatcaa
     7201 tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat
     7261 catgattgcc cacgaagcaa accgcacgca gcgaaagatt tctttgacag aagatgtagc
     7321 aaaagcattg atcggcagtg tgacctatgg aaatatagga caattgaagt caaatatcca
     7381 gttagtatgt gcacgaggat ttctaaatca tatgcaatca cccgaaatct cgatcacaat
     7441 cgatgatttg acagaaggga ttcgtagcgg attgattcaa ttagcaagca accgcagtgc
     7501 aatggcagag ctttctaaaa tattggagcc aaagataact gtagcaccaa atgatacgat
     7561 catgaaaatc caatcggact cctatgagtt accttataat ctatatgata tcatcggaga
     7621 taaagcagct ttactgaagt cggatggttt ggatcaagaa gcaatcaatc atttcatttc
     7681 tacagatatc aacatccatc tgaagtcttt ctataaagac catggttttt cttttaatgc
     7741 agacagcaaa ttagcagaat ttgtagatcc gaaaatcata gaagtgacca atcagattta
     7801 tgtaatggtc aaaagatctt taccatatga gtttcagcaa aactttattt atgctatgag
     7861 cttgcatatc agttcttttt tgaaaaaaat caacttagga gaagaacggc atacgaatga
     7921 taacatccgt gagatggctt ttgattttcc agatgaatat gctgttgcga aagaagtgcg
     7981 tagttatatc gaaacgtatt ttcaagtgga gattccagaa agtgaagact attacttgac
     8041 cgttctactc gtctcgttac gggcaaatca agcaagcggc agaatcggtg tagtcgttgc
     8101 agctcacgga aacagcacag caagcagtat ggtccaagtt gtccaacagt tgttagatgc
     8161 agagaacgta cgagcagtag acatgccttt ggatatggat ccgaaaacag cattggaccg
     8221 aattgaacgc aatgtacaag aagtagatga aggaagcggg gtcatactct tagtagatat
     8281 ggggtccctt gcgtcgttta attcgcaaat tcaaagagac actggaattc cagtacgtac
     8341 tgttgatatg gtaacaactt ctctagtttt ggaaactgta cgaaaggtct cagtattagg
     8401 gacagatcta gatatgttgc atgaatcatt gaaaaatttt cgaggttacg cagaaatatc
     8461 ttctgtagaa tctgttcagg aagcaaaacc tattcataag aaaaaagctg ttttggctat
     8521 ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa
     8581 caagcgtcaa gaaacagaat tagaagttct agctttgtcg attgtggaag tgaaagaaga
     8641 actgccagtt atccaaaaag aatatcagat catagctact actgggatta ctgatttgaa
     8701 tatccaagcc ccatttattc ctttagaacg ttttattgat caaaatatcg aagtcatttt
     8761 ggatcagttg ctgatggaat cagaattgga ggaaactgaa ttcatctccc tagatgaaga
     8821 gtcagctaaa aatacgtgtg ttgagtttat ctctgataac ttcatcttta ttaatggtag
     8881 taagttaatc gatccaatgt ggcaattttc tacacaaatc tctcagacga cgggaatcgg
     8941 cgatgaagaa tatggattca aaatcaacct agtgatgcac acagcaggaa tgatcgaacg
     9001 tattattcgg aatgaaccat tgactgtgga agaaaacgaa ttgacaaata caacgaatga
     9061 tcctctttat agccaattgg cagcctcagt tgttctgttg gaagatcaga taaaagtaaa
     9121 agtgccgata gaagaaatgt attatctgct acgtctagtc cataatcagt tagataaaaa
     9181 agaatacact gtgccatgaa tacacaaaaa cgatacactt ttatagtgta tcgtttttgt
     9241 gtattggtta agaatccttt aaattcaatt agtgtatcgt aaaaaaagtt ggcacgcttt
     9301 ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac agaaatgata ggggtttctg
     9361 tgaagtagtg aggaagaagg agttttgcag atggatattc gattggcacg tatagatgat
     9421 cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc aaacaggtgt tgaacgtatc
     9481 attgtgatca gcgatgtagt cgctcaagat aaattgagaa agtttctact aaaagaagct
     9541 gcaccgccgg ggatcacagc aaatgtaata accgtgcaga aaatgattca aatctatcat
     9601 agtggattat tatcacgagc aaaagtgatg cttttattca ccaatcccca agatgttgaa
     9661 gcggttgtcc gctgcggtgt acagttgaag tcgttgaata tcggcggtat gagttttact
     9721 gaaggcaaat caatgattac caattttgtt tcagtaaatc aacaagatat tgattccttt
     9781 cgttacttga acagtcaaga agtggagtta gagattcgaa aagtacctgc tgacaggaaa
     9841 atccttttga tggaactact agcgaaaatc aaaaaggtgt agcaagaatg gttgttggaa
     9901 aaggtcaaat aattctgttg taatggttag caattattaa tatttttgaa tttgttacaa
     9961 caaaagtaaa taggaggtat aaatatggta gggattatcc tagcaagtca tggacaattt
    10021 gctgaaggaa tcttacaatc cggttcaatg atctttggcg aacaagaaaa cgtcaaagcc
    10081 gtgattttga aaccaagtga aggtccagat gacttgagag ccaaattgga agaagcggtt
    10141 gcttcttttg ataatcaaga cgaagtgtta ttcttagttg atttatgggg cggaacaccg
    10201 ttcaatcaat caaacacatt attcgaagag cacaaagata aatgggcaat cgtcagcggt
    10261 ttgaatttac caatgttgat tgaagcctac gcatcacgct tttcaatgga atctgctcat
    10321 gaaattgcag cacacatcat tgaaacagca aaagacggtg taaaagtaaa accggaagaa
    10381 ttagaaccgg cagaagcacc gaaagcagca gttgaagacg cacaacctaa aggagcactt
    10441 ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg tattagcacg tgttgactca
    10501 cgtctgttac atggacaagt tgcgacagct tggacaaaag cagtgcaacc aaaccgtatt
    10561 atcgttgttt cagatgcagt atcaaaggat gatctgcgta agagactaat cgaacaagct
    10621 gctcctccag gtgtaaaagc aaatgttatt cctattagca aaatgatcga agtagcaaaa
    10681 gaccctcgtt ttggaaatac caaagcattg cttttatttg aaaatcctga agacgtattg
    10741 acagctgttg aaggtggcgt agacatcaaa gaattaaatg ttgggtcaat ggcgcattcg
    10801 gtcggtaaag tcgttgtaag taaagtattg tctatgggac aagaagacgt tgaagcgttt
    10861 gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta aagtacctaa tgattcacgc
    10921 gataacatgg atgatatttt gaaaaaagca aaagctgaat tagcaaaagc gtaataaata
    10981 atattaaaat ttataggagg cttatcatgt ctattatttc aataatttta gtcgtattag
    11041 ttgcctttct agctggtatg gaaggtattc ttgatgaatt tcaatttcat caaccactag
    11101 tggcatgtac attgatcggt ttagttaccg gtaacctcga agctggtatc gtacttggcg
    11161 gtacgcttca gatgatcgct ttaggttggg caaatatcgg tgccgctgta gcaccagatg
    11221 ctgcattagc atcagtagca tcagcaatta ttttagtttt aggtggacaa ggtgttaaag
    11281 gtgttccttc tgcgatcgcg attgccgttc ctttggctgt tgcagggttg ttcttaacaa
    11341 tgatcgtacg tacaatcgct gttccaatcg ttcatttgat ggatgcggct gctgaagaag
    11401 gaaacatccg taaggttgaa atgtggcaca tcattgctgt atgtttacaa ggggttcgta
    11461 tcgcggtccc agcagcagcg ttgctattta tcccagcaca aactgtgcaa tcattccttg
    11521 aatcaatgcc agcatggtta acagatggta tggctatcgg tgggggaatg gtcgtagctg
    11581 taggttacgc tttggtaatc aacatgatgg ctacaaaaga agtatggcca ttcttcgtta
    11641 ttggtttcgt tgtagctgca atttctcaat taacattgat tgctctaggt gctttaggtg
    11701 ttgcattagc tcttatttac ttgaaccttt ctaaaatggg tggttcatca aataatggag
    11761 gcggaagcaa ctctggtgac cctttaggcg acattttgaa tgactattaa tcttgaagga
    11821 ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa aaagatcgtt tagctgttgc
    11881 gtggcgctct accttcattc aaggttcatg gaactacgaa cgtatgcaaa acggtggctg
    11941 ggcattttct atgattccag caatcaaaaa attatacaaa acaaaagaag aacgttctgc
    12001 agcattaaaa cgtcacttgg aattctttaa tacacaccca tacattgctt ctccaatttt
    12061 aggggttaca ttggctctgg aagaagaacg tgcgaatggc gcgcctgttg atgatgtagc
    12121 gattcaaggg gttaaagtag gtatgatggg tcctttagct ggtgttggtg acccagtgtt
    12181 ctggtttaca gtacgtccaa tgctaggtgc actaggtgct tcattagcta tgggaggaaa
    12241 tatccttgga ccaatcatct tcttcttagg ttggaacatt attcgttggg cattcatgtg
    12301 gtatacacaa gaatttggct acaaagctgg ttctaaaatt actgatgacc tttctggcgg
    12361 attgttacaa gacgtaacaa aaggtgcttc aattttaggg atgttcgtat tagctgccct
    12421 tgtacaaaga tgggtatcaa tcaaattctt accagttgta tcaactgtaa aattagataa
    12481 aggtgcttac atcgaatggg ataaattgcc ttcaggtggt gaaggaatca agagtgcatt
    12541 tgaacaagtt aataatggct tagccctttc tcctgaaaag gtaacaacat tacaagacaa
    12601 cttagaccaa ttgattcctg gtttagctgc attaggatta acattgttat gtatgtggtt
    12661 gcttaagaaa aaggtttctc caatcgttat catccttggt ttattcgtag tcggtgttgt
    12721 tggacacgtc atcggacttt tataatccat aaaaatatag ctcgggcgtt gtcccgagct
    12781 ttttttatat agtatactgt tcataaacaa atatctggag ggagaaaatg gttcaatcaa
    12841 tcaatacaaa agtagacttg gtaattgatg ctacttcgtt tacgggtctg acagattacg
    12901 ggcaaatcat gataggtaat aatggttttg agttttacca tgcgcgagat agacgcaaat
    12961 atatccaaat tccatgggaa gaagtggatt atgtactagc atcagtcatg ctaaagggaa
    13021 aatggattcc tcgatatgcc attcaaacga aaaaaaatgg tacatacaca ttttcttcca
    13081 aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt agatgaagat agaatggtac
    13141 aatcattgag tttctttgat gtagtgaaac gtggtattcg tagtatgttt aaacgcaagt
    13201 aaaaaataaa gaaaacttct aagaggaggt tttctttatt tttttacttt gcagaagaaa
    13261 atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc ccatatgcta aaattgatta
    13321 gcaaagaagg aggttatatt atgagttggt tatggacatt gattgttgga gccgtgatcg
    13381 gagcaattgc aggtgcgatc accagtaaag gaaattcgat gggctgtgta acgaatatca
    13441 ttgctggact agtcggttca agtattggac aagcgatttt cggcgaatgg ggaccaaaag
    13501 ctgcaggtat ggcacttatt ccatctattt taggcgcagt gattttggta gcagttgtct
    13561 catttttctt tggaaaacga tcataagttt ttacaaaata gtaaactaaa aggagttgca
    13621 gcgagactgt gactcctttt tggctttatt tatttaaaat aaaaagaagt tttccggaaa
    13681 tttttattta actgtcgtgt acgaagaaaa gtttgctata atggaaaaaa gcgtgcaaag
    13741 aaagagggag tttcattatg atgctacgtg tagaaaaatt gcgcaaaaaa atgcaagaag
    13801 aaaatttgga ttcatttttg gttaccagtc cgtacaatct tcgttactta acgaacttta
    13861 caggtacgac aggattagct gttattactt tggaaaaagc attttttatt actgattttc
    13921 gttataccga gcaggcggca gcgcaggctc aagggtttga aattattaaa aatgtcggac
    13981 ctatttttga tgaggtagca aacttagtac gcaaagaaga actgcactca ttagcttttg
    14041 aggaaacaac agtctcattt ttagaatatt cggttttaga agaaatcatc gatgcggaat
    14101 tagtacctgt ttcacagatg atcgaagaac tcagagaagt gaaagatgaa gaagaaatcg
    14161 ctattatcga aaaagcatgt catattgcgg atatggcata tgatcacatc ctgaaaatga
    14221 tccaaccggg catgactgag attgaagtcg caaatcaact agacttttat atgcgttctc
    14281 ttggcgcttc aggtgtttct tttgatacga tcgttgctag cggattgcgt tcggctatgc
    14341 cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga cttgatcaca ttggactttg
    14401 gttgctatta cgaagggtac gtttccgata tgacacgtac attcgctatt ggtgatcctg
    14461 gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc tcaattagca gttattgatg
    14521 ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt tgctagagat tatatttcaa
    14581 aacatggtta cggggaagca tttggacatt ctacaggaca tgggatcggt ttagagatcc
    14641 acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt tgtaccagga aatgtgatca
    14701 ctgatgaacc agggatctac ttaccaggaa tcggtggagt acgtattgag gatgacttgt
    14761 tgatcacaga agatggaaat cgtgtgttga ctcgttcacc aaaagaattg atcatcttat
    14821 agtttcatca atagaagaag taagcgtaaa aatcagtgtt cacagacttt gttatagaaa
    14881 atcactcgat ttaatgatac actatagaag aacttttaag ctgtaagaga taataaagtt
    14941 gggtgttttc tccaattcat agcagtttgc agctgatcgg cggcagtttt cttaccacgt
    15001 gatcactatg ccgcatcata gagaattcgg atgtatctcg tgcttaggca aagggaggaa
    15061 taaataaatg gcagatgaaa aaaatttgat tttaaacgca aaccaagatt taggagagat
    15121 cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt gcagcttcta aagtagaagg
    15181 cgtatacgga atgcgcggaa cttttgccag caatgtcact gaattattag gacgtgcagc
    15241 tcacggaaaa ggtgtttact tagcaaacga ggaagatggc ttgaaagtag atctttactg
    15301 ttatctagaa tatggtgttt cagttcctaa agtagcaatg gaaatgcagg aacgggtcag
    15361 ccagcaagtc cttttcatga cggatatcga tttatctgaa gtcaatatcc atgtagtagc
    15421 tgttgttcct gaaaaattag aacaaccaac aatcgaagaa ctttttgaag acgaagagga
    15481 agagaatgaa taaagaatta tcaagacacg agattcggga aatggcattg caggctttat
    15541 ttccattaga cttcaatgca gacctaacaa aagaagatgc gattttcaac gcaatcgaat
    15601 tagaccaccg cgacatgatc aacgaagatg aatcagagtt tgttcctgtt tatcttgata
    15661 cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga taaagtgatt gaaaaacatt
    15721 taaaaaacaa ttggcacatc aatcggatct caaaaatgga tttggtgatc ttgcgtatcg
    15781 cgatttttga aatgacttat gtgacagatg taccagcggc tgttgcttta aatgaagcaa
    15841 tcgagttggc aaaaacattt agtgatgacc gttcacgtaa attcgtcaac ggtgttttat
    15901 ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct agcttttttc tatgaatcat
    15961 cataaacctg ttataaatcg ggaaaattct atggtagaat gaaaacacaa aaaatatctg
    16021 ggagtggtta gatggcagaa ttgattaacg gaaaagaatt agctgaaaaa atgcaggcag
    16081 agattgctga aaaaatccaa aaattgaaag ataatggtat ccatcccgga ttagtcgttt
    16141 tattggtagg ggaaaatcca gccagccaaa tctatgtaag aaataaagaa cgtgtagcaa
    16201 aagaaatcgg aatccattca ttggtagagc gttatcctga gacgatttct gaagacgaac
    16261 tacttgcaga aattgaaaaa tacaatcaag atcctagttt tcatggtata ttggtccaat
    16321 taccattgcc aaagcatata gatgaagaaa agatcctgtt agctatccgc cctgaaaaag
    16381 acgtagacgg ttttcatccg atgaatttag gcagactcct tgcgggtaaa ccggacaaga
    16441 ttccttgtac tccatacggc atcatgaaaa tgtttgaagc ttattctatc gatccggcgg
    16501 gtaaacgagc agtagttatc ggacgaagca atatcgtcgg taaaccgatg gctcagctac
    16561 tcttgatggc agatgcaact gttactgttg ctcattctaa aaccgaaaat ctggctgaac
    16621 tggcacaaga agcagatatt ttagtcgtag caataggacg tggacatttt gtcacaaaag
    16681 aattcgtaaa accaggtgct gtagtgatag atgtagggat gaatcgtgat gctaacggta
    16741 agctgatcgg tgatgtgaaa tttgatgaag tggagcctat tgcaggctgg atcactcctg
    16801 tgccaaaggg tgtagggcca atgactatca cgatgctgat gtatcaaacc gtaaaatctg
    16861 ccgaagcttt tggagcaggt gaatagatgg cacaagaata tttaactgtc actacattaa
    16921 cgaagtattt gaaaagaaaa tttgatgccg atccttattt agaacgcgta tatttaacag
    16981 gcgaaatatc gaatttccgt ttacgtgcca atgcccatca atatttcagc ttaaaagatg
    17041 accatgcaaa aatatctgct attatgttca aaggagcatt ccaaaaatta cgattccagc
    17101 caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc attatatgaa gcgggaggtt
    17161 cttatcaaat ctatgtagag cacatggagc ctgatggaat aggtgcttta tatcaagaat
    17221 tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt tcaagggcct aaaaaaatgc
    17281 tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc aagcggagcg gttatccgcg
    17341 atatcatcac gacggtgaaa agaagatatc caattgtcca attggttttg ttcccaacag
    17401 ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa tatccagaga gtagaagaga
    17461 tgggcaattt cgatacaatg atcatcggtc gtgggggtgg ttcgattgag gatttatggc
    17521 cgtttaatga agagcgggtt gctcgggcga tttatcaagc taatacgccg gtgatttcat
    17581 ctgtaggaca tgaaacagat accacgatcg ctgatttagt agcagatgtt cgggctgcca
    17641 ctccaacagc agctgcagaa ttagctgttc ctgttttgtc agaagagttg atgcgcattc
    17701 aagaacggca agctagattg cagcaggcgt ttgtacgcca gatccagcgc aaacaagaac
    17761 ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc tgaaagattg tatgaaggtc
    17821 agaccatcaa attagaccag ttgaaacagc gattatatca agctacacag caaatttatc
    17881 atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt ggaacaagtg gctccgcttt
    17941 atcgagttaa agcagctcgc caagaaactg cttatctaga aaaacgctta gaagagaaaa
    18001 tccaacaata tatgaaacaa cagcagcagc gttttgctca agctgttcaa tctcttgatt
    18061 tgttgagtcc attgaaaatc atgggaagag gctacagcta tacgacgtta aatgatcaag
    18121 tagtaaaaag tgtcaccgag attcatcaag gggatttgct gacgatccat tatcaagatg
    18181 gaatcgttaa aggacaagta caagagatag acaaaggaga ataaaaatat ggaaaatcca
    18241 acttttgaag agtcactgca agaattagaa aaaattgtca tgcagttaga acaaggagac
    18301 gttccattag aatcagctct agattcgttt aaacgaggaa tggaattaag caaacattgc
    18361 caagatacat tagctaaagc agaagagacc ttgacgaaaa tgatgacaga atcaaatgaa
    18421 gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga ttaactgact tttcagctat
    18481 tcatctacct gctgtagaaa aagaaatctt gtctttttta gatgagcata caacggatag
    18541 aggactctat gatgcgatga gttattcagt gaaagcaggc gggaaaagaa tacgtccctt
    18601 attactttta acggccgttg cttcctttga tgagcctatt gatgttcccg tctatcaagt
    18661 agcagctgct ttagaaatgg tgcatacgta ttccttgatc cacgatgatt tgcctgcgat
    18721 ggacaatgat gatcttcgta gaggaaaacc taccaatcat aaagtattcg gcgaagcatt
    18781 agctatattg gccggagatg gtttgcttac aggagcattc cagctaatca gtatggcaca
    18841 cttaggtaat tctcctaagc ttctgctgct tcagcaatta gctgtttgtg cgggatcaca
    18901 aggtatggtt gctgggcaag ctgcagatat cgagggcgaa tcgaaaaagc tttctttaga
    18961 agaattagca tttattcatg aaagaaagac agggcatttg attcgttatg ccttgctggc
    19021 tggtggcata ttagcgaagc aacctgaaga aatcttattg ctacttcaac gattagccga
    19081 acatttaggt ttagcttttc agataagaga tgatctactc gatgtgattg gtacaacgaa
    19141 aacattaggg aaaacagctg gtaaagatga acgtatggaa aaaaatactt acccgcgttt
    19201 attaggccta gaaaagacac gtgaggcgtt ggagattgag ctgttatccg ccaacaagat
    19261 catagacaaa ttagaagagg acgttttttc ttttgatggc gggctgatgc gccagatgat
    19321 caagcaattt gatgtggaga aatgagggaa agaatggaaa aagaacgcgt agatgtatta
    19381 gcggtcaaac aaggattatt tgaaacaaga gaacaagcca aacgttcagt gatggctggt
    19441 ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg gagaaaaaat tccagtgagt
    19501 agtgaattaa gagtcaaagg taaaaaactt ccttatgttt ctcgaggggg attaaaatta
    19561 gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa aaacattgtt agatattggt
    19621 gcatccactg gcggatttac tgatgcagct ttgcaaaatg gcgcaaaaat gagttatgct
    19681 ttagacgttg gctacaatca gctagcttgg aaaataagac aagatcctag agtcgtcgta
    19741 atggaaagag tgaattttcg ttatgcaaaa cccgaagact ttaccttagg aattccagaa
    19801 gttgctgtta tcgatgtttc ttttatttca ctaaaactga tgctgcctcc attacatgct
    19861 attttaaaag aagagggaga agtgattgct ttaatcaaac cccaattcga agcaggcaga
    19921 gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga cccatcaaaa agttttagaa
    19981 gacattttgt catttgcggc acaaggtgga tatgatgtgc tggaattaag ttattctccg
    20041 atcacggggg gagaaggaaa tatcgagttc ttggctcact tgaggaaagt acccgaaagc
    20101 ggaacaatca attcagctat taatatggca gaagtggttt caaatgcaca cgaacagttt
    20161 gaccataaat aaattgtaac tgtgaaaatc aagtgagaag tatgcggatt cttctttatt
    20221 ataccaagga aataagctat cattgattaa gaagaatatg ctaaggagcg aagacgtaat
    20281 gagaaaaaaa gatcggcatc gtttgattac tcgattgttg agtgagcaag atatccgaaa
    20341 gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata tctgttactc aagctacgat
    20401 ttccagagat ataaaggaac tgaaactgat caaggtaccg gcttcagatg gcggctaccg
    20461 ttatagcata ccagctgaaa caaacgaaga tgtaaatact aaattggaaa aactattaaa
    20521 agacgctttc gtggcagttg accaaatgga aaaatttgtt attttgaaaa cattgccggg
    20581 aaatgcttcg gcagcagcaa atttgattga taaacgttac aagaaagaat tattttcgat
    20641 tatcaatgat gacgataatg ttttgatgat tacacgaact gaagaccagg cagttttatt
    20701 gaagaaagac ttcctgcatt acctttaagt tgaggagcga atgtgatgct acaagaaatc
    20761 agtattacta actttgcgat tattccggaa ttgcggcttt cttttcacga aggaatgacc
    20821 gcgctgaccg gagagactgg tgcgggtaaa tccattatta tcgatgcttt aggtctgtta
    20881 gcaggaggaa gaggttcaag cgattatata cgtcaaggag cagaaaaatg tgtgcttgaa
    20941 gggttgtttg aactaccaaa acaagaagga ttctctgaac tgatggttga attagggatc
    21001 gaaacagatg aggacaatct gattgttcga cgggatatgt ccttaactgg aaaaaatgtt
    21061 tgtcgagtga atggtcatat tatcacatta gctaatctga gaagaatcgg cagttattta
    21121 gtagatatcc aaggacaaaa cgaacatcag gaactgctcc agcctgaatc acatcttgct
    21181 ttattggatc gttttggaga tgtagctttc caacagaaga aaaagagtta tcagcaagaa
    21241 tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag tgcagcaaaa tgaaaaatcc
    21301 tatgttcaga gaatagatat gctccatttc cagcaagaag aaatagctgc tgctgatttg
    21361 caagtcggag aagaagagaa attaaaagaa gaaagagaaa aacttagcaa ttatcaaaaa
    21421 attgctgatg gattagctgc aggttacgga gcactgacga ataatgaaca aaacagtgta
    21481 gatggtgttg gcttggccgt ttcagaactg caagggatcg cccatctaga tgtggagtat
    21541 gaagcaattt atgaaaatat ccaaagtgct tactatttgc tgcaagatgc aatcggcgat
    21601 atgagtcgtc agattgattt attggagtta gatgagagtc gtttagaaga agttacgcaa
    21661 cgtttagaat taatccgaca gttgaaacgt aaatacggcg aatccattga gtcaatattg
    21721 gcttactatg acgaaatcac cgaagagcta gcttcttctg atttttcaga aggtcaattg
    21781 gacaaaatga aagaggaact agagcaaaaa gagcttctac ttcagcaaca agccgcagat
    21841 cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa aatccattct gcatgaattg
    21901 aaaagtctgt atatggaaaa cactgagttt gaggtacgtt tcttaaaaga agaaaataga
    21961 caattgaatc atgatggatt tgaccaaatc gaattttaca ttacgaccaa tccaggagag
    22021 ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac tgtctcggat gttgcttgct
    22081 ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta ttatttttga tgaagtggat
    22141 acaggtgtga gcggaagagt tgcacaggcc atcgcagata aaatttctca aatatcgaaa
    22201 tactctcaag ttttgtgcat cactcatctg cctcaagttg ctgcagtcgc cgactatcaa
    22261 tattatattg tgaaagcagt gattgatgga cgtacgcaaa cttctgtttc agagttgaaa
    22321 acaaaagaaa gagaagaaga aattgcacgt atgctggctg gaagtgagat tacaaaactc
    22381 acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa ggacctgagc cacctcgcag
    22441 tggttcaggt ccttttcagc agctgatagc tatattttat ttttacatga cactcattac
    22501 agcagtcaat acaacagatc caatcgttaa aatcagcatt gtccaaatga caaattttgt
    22561 tactttagca aaagtacttg tttgtttttt ttcacgcatt gttttcaacc tcgtcattaa
    22621 ttagttgatt caagtgtaca atcttttttc catttccaca agagggcttt tttataaaaa
    22681 cttccctttt tttaaataat aggtagttac gatacaaata aataatgtta atagaactag
    22741 ccatataaag gcatcatccc tttcagcaat aggtaactta atgttcattc catagatact
    22801 gccgacgatt gtcggtatag ttaaaataat cgttaaagaa gttaaaattt tcataatatt
    22861 attcagatta ttcgatacga ttgctgaaaa agtctgattc atttgttcta ctagttttaa
    22921 ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc aatacatcac gtaaatgatt
    22981 tttcaaagca tgattttctt ggaaaagttt attttgcgct agttctttca atgcatctaa
    23041 attagcagaa gtcgcttctc tgaaataaac gaggcttttt tggatatcca tgatttgata
    23101 aagttgtttg ttttcagtgg atacttgaag tttggcttcc aaagtatcgc attgagattg
    23161 aagcgtagct aaataactgt tgtaagagag tgtgacctgc caaatgaatt gaaagaagat
    23221 attgagatta ggcgaaatat cactttttgg aaaggtctgg ctaaagactt tctttaaaaa
    23281 cagtggttca tgattcgtaa ttgtgataag cttctttttg ggcgtcaaaa taatagagag
    23341 cgggtatgta tccacttgaa aataaccgct aggacttgtt ttagcaaaag gatattgtaa
    23401 caataataac gctggctttg tcaactttga ttgattgaat tcttctgtac gggaattttc
    23461 tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt tcaaattgat gtgtcagctg
    23521 atgaatctct tcttgattag gacgttctac acaataccag atcgtttggt cagtttcctt
    23581 tccggtatta cgcagtttct cattatctaa tgtaaaatat ttgatcatga ttttcctcct
    23641 gttgcctgtt tcttttatta taacggatag ggaattggaa tacatggaat gtgtttgtaa
    23701 gatatagaat ataactgcat aaatttaaat aaaatgaaac agttgtgtaa agtatataca
    23761 gtatacttga agcaacgata aaaaaggagt ttacgttcat gggcacattc acaccactta
    23821 tcttgttttg gtatatttac ccggtaatcg ttttgtttgg ttgccaattt cttgtaaaat
    23881 tattttcgtt aaatcgtcgt tttaaaataa aagcccctga tttggcgatt ccttttttgt
    23941 ggggcggtat gcatgcattg tctagaaata cagggacaat ttctatctta ccgtttttct
    24001 ttatttctgt tttgctaatg ggaatttgta ttgctgtttt ccaagcatat tattatgaag
    24061 aaattattta tccgcgttat ttcaagatga cttggcgttt ggtttttctg ttgacaatcg
    24121 tcttgtacgc tgtcttgatc attgtgaaca tactttctta cctatgaaaa aaatagaaaa
    24181 gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa tgtttatgaa
    24241 taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg attgtggtag
    24301 actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga attccaacat
    24361 aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga acttggcgaa
    24421 aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat gagcgaatgg
    24481 gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc acgtactttt
    24541 gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg acgaatcaat
    24601 atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt catcggtgta
    24661 tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc agcagcagaa
    24721 gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga ataggaagag
    24781 gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa acggtagatg
    24841 gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt gccggacata
    24901 gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt gaccaagatc
    24961 aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa ggtatggtca
    25021 catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat gtagataagg
    25081 tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa gcagaacgtg
    25141 gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca gctcctttat
    25201 ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt ttcttccgct
    25261 atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta cgaaaaacaa
    25321 aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc ccagctcccg
    25381 caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg attgcagtaa
    25441 atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatccggtta ttgaaagtca
    25501 atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc aaaagtatgt
    25561 ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata cctgacgaat
    25621 tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag gaagagctaa
    25681 cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata agaatgaaag
    25741 aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga tttacctgaa
    25801 ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga aatcattctt
    25861 ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat tgctgtggca
    25921 agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg cacaaatatc
    25981 agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa aaagcaagaa
    26041 aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat taaacaaatc
    26101 gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa gtaaatgagt
    26161 cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat gaacaatcgc
    26221 aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt atttgccgtc
    26281 cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc tgaaaaaaca
    26341 aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat ttatgatcgt
    26401 aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat actctcaaaa
    26461 acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga taaaatagca
    26521 gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat tttagaggac
    26581 ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa tatctcccaa
    26641 gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg tctttatttt
    26701 gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt tattggatac
    26761 gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga gtctgcttac
    26821 aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa ttttcaaaat
    26881 cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga tatctacacg
    26941 actttagaca gcagactaca aagttattta gaaacactga tggatcaagt aaatgaagaa
    27001 tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga aatcttggca
    27061 atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg aaaagatgct
    27121 atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat gaaagttttc
    27181 actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt ccaatctgga
    27241 aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa aggtgtgctg
    27301 accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact ggaacagcga
    27361 ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag tactcattct
    27421 ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga ccgagcaatg
    27481 agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc tttcacttct
    27541 attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt ggatccccaa
    27601 actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc aaaagaaaca
    27661 acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta cggtagtgct
    27721 tatggtgtat atagtgtacc tggatacaat gtatcggcaa aaaacggaac agcccaaatc
    27781 gcttctgata cagggggata tcaaacagga gatacagcct acctgtattc gattgtggag
    27841 atggttcctt ctgaagatcc agactatgtc ttgtatctaa caatgaaaca ccctaagaca
    27901 tatgatcgaa tggcattggc taaaatcgca aatccattga tgaaacgggc aatggatttt
    27961 aaagaaactg aagaagattc agatacagaa acaaaaacag aaaaagtatc cgtagccgat
    28021 tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg actgcaacct
    28081 gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg tgatcagttg
    28141 atctctggag aaaaactgat cctatatact ggtggtgata agctgatgcc tgacgtaaca
    28201 ggatggtcaa aagcagatat catgaagctg gggaaaatcc tcggtattga agttagtttt
    28261 gatggagatg gttattgtgt caaacaagaa cttgctccat atgaaaaaat cactgaagac
    28321 aaacttagtt ttactttaga agaataagaa gagaaatagg agagaaaaga gcatggaatg
    28381 gacacaagca ttgattccca ttgtcagcag ttgtgcaatg acaattgccg caatgccgct
    28441 atttattggt tattttcaaa tgaaaaagca aggtcaggcg attcgtgagg agggaccaaa
    28501 gtggcacaat agcaaagccg gaactcctac aatgggcggc ttagtgtttc tgatcggctc
    28561 tattttgaca ggaatttggg ttggagcttg gcaaaaacag ctaactccta ctcttttcat
    28621 cttgcttttt gtattagcgt tatacggagt aatcggcttt cttgatgatt ttataaaaat
    28681 attcaagaaa agaaacatgg gactgaactc gaagcagaaa ttgattggac agattctagg
    28741 gggaatcatc ttttatcttg tttaccgttc tgaaggttat ccaggtgtat tgaatttctt
    28801 cggactagat ctacctttag gaatcattta tggtattttc gctctcttct ggcttgttgg
    28861 attttcaaat gcagtaaatc tgacagatgg catcgatgga ttagttgctg gcttaggaag
    28921 tatctctttt gcagcttacg gtctaattgc ttggcatcag catcagtatg atgtattaat
    28981 catttgctta agtgttttag gaggtttact gggtttcttc gtctacaaca gaaaacctgc
    29041 caagattttt atgggagatg tcggttcatt agcgttgggc ggacttttag cggcaatttc
    29101 tataatgcta aaccaagaat ggacattgct tttgattggt ttgatctatg ttatggaaac
    29161 agcaagtgtg atgcttcaag ttacttcctt taagcttaca ggaaaacgaa tttttaaaat
    29221 gtctccaatt catcatcact ttgaaatgtg cggatggtcg gagtggaaga ttgatacagt
    29281 tttttggttg atcagcattg tgacttcgtt gatcacatta tggatcgttt ggtaggagat
    29341 gacgggaaat gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc ttaggactcg
    29401 caaaaagcgg agtaagtgcg gccaaattat tacatgaatt gggcgcactt gtcaccgtta
    29461 atgatggaaa accttttgac gaaaatccgg aagcacaaga gctgctttct ttagggataa
    29521 aagtaataac aggtagtcat ccaatcgaat tgttggatga agaattttca ttgatggtca
    29581 aaaatccagg gattccctac agtcatcctt tagtagcaaa agcccaagaa atgggtattc
    29641 cggtaattac ggaagttgag ctagcttacg aagtagcgga atgcccaatt attgggatta
    29701 caggaacgaa cggaaaaaca acgacaacaa cgatgacagg actactactg aatgcaggag
    29761 cagatcaagg aattgcacgt ttagcgggta atattgggta tccagcaagt ggtgtagcgc
    29821 aagaagcaaa atcagaagat aaaattgtga tggaattgtc tagcttccaa ctcatgggaa
    29881 tcacagattt ccgccctcat atagcggtga ttaccaatat ctatgaagca catattgatt
    29941 atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa aacatgacag
    30001 aaaaagatta tctgatcctt aactggaatc agtcagaact acaagaatta gcgcaaagaa
    30061 caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt gtgtatgctg
    30121 acgattactc aatatactat aaaaaagaaa aaataatgga aatatctgaa cttggcgtac
    30181 caggtaagca taatgtagaa aatgcactag ctgctatttc tgtagcaaaa ttgtatggta
    30241 tatcaaatga agctatacga gaaacgttgc attttttcca tggcgtacca catcgtacgc
    30301 aatatgtagg agaaatccaa ggcagaaaat tttataatga ttcaaaagca accaatattt
    30361 tagcgactaa aatggctcta agtggatttg aaacgtctaa ggtcgtgtta ttggctggcg
    30421 gattagacag aggaaataca tttgatgaat taattccttc attaaaagga atcaaagcaa
    30481 tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa gcaggcatcg
    30541 agacaatagt aacagccgat tctgtagaac aagcagttcc tttagcatta gaaaacagta
    30601 cagatggtga tgtagtttta ttatcaccag cgaatgccag ctgggatcaa tacccaaatt
    30661 ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg attaaaatag aaaaagtaga
    30721 agcgagcgat aaaaaatgaa aattttggtt acaggcggag gaacaggggg acatatttat
    30781 ccagctcttg cctttgtcaa ttatgtaaag actcaagaac cgaatgctga attcatgtat
    30841 gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg atacaggaat gcctttccat
    30901 accttggaga ttcaaggatt taaacgaaaa atttccatgc ataacatcaa gacggtccga
    30961 ttgtttctta agagtatccg tcaagcaaaa aaaatcctca gtgattttca accagatatc
    31021 gttatcggta ctggaggata tgtttccgga gcggtagtgt acgctgcagc caaaatgggt
    31081 attccaacag ttatccatga acaaaacagt gtaccaggtg ttaccaataa atttttaagc
    31141 cgatatgtgg atcggatcgc tctttcattt aaagatgcgg cttccttttt tcctgaaaac
    31201 aaagcagttc ttgtaggtaa tcctcgagca caagaagtag cagatactaa aaaatcagat
    31261 attttgaaaa catttgattt ggatccagaa aagaaaacag tcttgatttt tggcggtagt
    31321 caaggagcac ttaaaatcaa tcaagcagta tcagtttttt taagtgaata tattccttct
    31381 tcttaccaag ttttgtatgc ttcaggagat agatattatg aagagatcct aaataaaata
    31441 aatcctgaaa tacaaaatat tagtgtccag ccttatatca aaaatatggc agaggtcatg
    31501 gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga attcacagct
    31561 ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca tcagactaaa
    31621 aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa tgaactaaca
    31681 ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt gcaaaagcaa
    31741 atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct ttatgactta
    31801 gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa aaagaaccaa
    31861 aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa aaggctaacc
    31921 gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa gacataacat
    31981 tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa cagcaagaag
    32041 aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag aaaaacggtg
    32101 gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa cggcacaaag
    32161 tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg atttttttga
    32221 tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc aataagacag
    32281 tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat gtttggggac
    32341 agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca agaatcgaaa
    32401 cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca gaatataaag
    32461 agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat ggaacagtat
    32521 tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag ttcaaagact
    32581 ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa ttacaaaaag
    32641 caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc caattgaata
    32701 tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa atgaaatatt
    32761 attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa gttggtatct
    32821 tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg gaagaaacaa
    32881 ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca gaaacgcaaa
    32941 aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat gattcaagtg
    33001 aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat gaaaattgat
    33061 aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat ggtagaattg
    33121 gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt tgttcgcttg
    33181 tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa tgtatgtagg
    33241 ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg acagccaaat
    33301 gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca ttatcgttga
    33361 tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag aaaaagcagg
    33421 tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag tagaaaattg
    33481 ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag atgtcagaaa
    33541 tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg tgtcaatatt
    33601 gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg gaatgatcgg
    33661 cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca tccataacat
    33721 acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta ctcctttagc
    33781 gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg tcattgatat
    33841 gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt ttaccaatct
    33901 tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa atacgtcatt
    33961 taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac ggacatcacc
    34021 agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag tcgatgaacg
    34081 ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag caaaagatgc
    34141 attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag gcggagctgc
    34201 tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg taaaattgta
    34261 tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa gtatcgtgga
    34321 ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta cgggtgaaac
    34381 ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg agagctatga
    34441 agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag aaaatgtaaa
    34501 aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg gaggaataag
    34561 atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa gtcatcggag
    34621 tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt aaaggtgttg
    34681 aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct gaaacagtta
    34741 tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca gaagttggac
    34801 aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt gcagatatga
    34861 ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca atcgttgcag
    34921 gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca ttcacttttg
    34981 aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa gaaaatgtgg
    35041 atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa aagacaccga
    35101 tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa ggtatctctg
    35161 atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa acagtgatgg
    35221 aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat cgcgtagtag
    35281 aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat ggagctgaac
    35341 aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca caagatgctt
    35401 cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt acatctatca
    35461 atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc gatgaatcaa
    35521 aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca cctgctcaaa
    35581 agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa aacagttttg
    35641 gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat tccaattttg
    35701 acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa tctcatggcg
    35761 atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga ggaagagcac
    35821 atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg tgctcttgtt
    35881 catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga atccgctcaa
    35941 gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt agataaatta
    36001 ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt aattggtaat
    36061 ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca tgcgttggac
    36121 agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc ctgttttatt
    36181 gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga gcttttaccg
    36241 tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat gactatggcg
    36301 cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa agaattgcgt
    36361 gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag tatggggatg
    36421 agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt aggaacagcg
    36481 ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag gagcaattgc
    36541 taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc ctgaaacaac
    36601 aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa ctcattctgt
    36661 atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg taacgcatga
    36721 aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac ctacaaaaac
    36781 tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa atcttgctgg
    36841 aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg ctaaacgaat
    36901 cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac aagcgagaag
    36961 aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca aacgtgttgg
    37021 agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa ccgctcaatc
    37081 ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta tactattgca
    37141 ctattacttc atttacttca acgggcagcc tatttctata gcattatctt gatggtatat
    37201 gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt attagctaag
    37261 atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg acccttggat
    37321 ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt agcacagatc
    37381 gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct ttattctcag
    37441 aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg ggcagatgaa
    37501 cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca atatgcaccc
    37561 tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact gattcgacaa
    37621 agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag aaaaagatgt
    37681 ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc attgtatgaa
    37741 atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg aactttagtg
    37801 ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga tcgctggcaa
    37861 gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa gattggaaag
    37921 gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa agatagctgg
    37981 acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc tgagatttat
    38041 aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa agtgaactgg
    38101 acagaaaata caagacctga ttttgtactt gaactattag atattatttc agtcagaggt
    38161 tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa gtacagggta
    38221 ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct ttaactccat
    38281 tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa gacgaagtag
    38341 atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga aatcgagaat
    38401 tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa ttaaaagatg
    38461 ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa acaagtgcaa
    38521 gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag ttagttgcga
    38581 acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca agaaaaatct
    38641 tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat cttaagaaaa
    38701 aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta gaacaagtga
    38761 aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact gacagccatg
    38821 aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact gatgtaaact
    38881 cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct attgatatct
    38941 cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt tattcttaaa
    39001 gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc ctctacttga
    39061 gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt ggtcacagag
    39121 agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt cgtcccttgc
    39181 ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt tgaatgaaaa
    39241 tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat ttgccaaacc
    39301 gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa agacaagagt
    39361 tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc aatggtaata
    39421 tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt tcaaaatcaa
    39481 tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg ccgattgaac
    39541 aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa tacttggaaa
    39601 aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc aaacgcctag
    39661 gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat gaagctgcac
    39721 aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga ttgaaaccaa
    39781 tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa tataaagatg
    39841 taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat attcttgatg
    39901 aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca aaccaaggta
    39961 ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt aagtttgtcg
    40021 tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat gtaacagttc
    40081 taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcca ttctatgaac
    40141 gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact ggattagtcc
    40201 atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat aaactatctg
    40261 tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga tttgaaggta
    40321 tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa ggagctttgc
    40381 tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa aagcctgtga
    40441 tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag aatattttgg
    40501 atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg tataatatga
    40561 tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta ccacttccga
    40621 ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa catgtggcaa
    40681 aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa gaacttcttc
    40741 ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa aaagatatca
    40801 tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag cgaccagaat
    40861 tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc tggttcaact
    40921 caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca gttatttctc
    40981 aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga aacatcattg
    41041 caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg gtatctagtg
    41101 tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg gcagaagtat
    41161 atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat ttcgatccta
    41221 aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg accgttcgat
    41281 tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac ttcatgcata
    41341 tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac ctggattttg
    41401 ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc atgcaaactg
    41461 ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt ccgcatacag
    41521 cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta agcgagtttc
    41581 ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact gctttcatgg
    41641 acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa ttgattggta
    41701 aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa atgttaaaag
    41761 ctttggatgc agatattgca caattattga tcgtttcacc agactacttt gaagttatgg
    41821 cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct attacagtgg
    41881 aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat gtaggagtag
    41941 atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac ttctatcctg
    42001 aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg acatgttctt
    42061 tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata ccagttatct
    42121 ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc
    42181 attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg
    42241 acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt cgttccatca
    42301 ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc aaccatttct
    42361 gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc tacttctttc
    42421 ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag aacattaggt
    42481 ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt aacaggaact
    42541 tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca
    42601 tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc gacgaatgtt
    42661 tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa ttgtacacca
    42721 tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta
    42781 taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt ttcggaaaat
    42841 gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt ttgcaccatg
    42901 tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc gaggttttcg
    42961 ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc aaaaatatta
    43021 ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata atgatcaatc
    43081 ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc aaaagcagac
    43141 tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt ttttgtcata
    43201 atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc catagccaaa
    43261 taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga caattctttt
    43321 aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa atggcttgca
    43381 aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt gacaacttca
    43441 cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa atgctcgcct
    43501 aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg ggctaggtca
    43561 cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg gatacactcc
    43621 tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg attttctttc
    43681 tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc tcatattctt
    43741 cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca tcaagcactc
    43801 gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg cgttcttcat
    43861 ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca ggatgattca
    43921 aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat acttcagcat
    43981 catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact tcccataagc
    44041 gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat tgtacacctt
    44101 gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt tcactgacag
    44161 atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc gttgtatctc
    44221 cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata ttcgatcatc
    44281 ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa ttgactattt
    44341 ttctaaataa aaaacaaagg atttttagac gtttttacaa caaaaaacga aagatgatag
    44401 agaagactca ttctttcgtt tataaaggat atatatatac attgggaggg atctattttt
    44461 cagataacaa cttatatgaa gtgtctgcag attcgtcata aagagtttgt tcgggataag
    44521 aaaagataag ataaaaacca tactgatcac gtacgactaa acgatattca tctatttcta
    44581 ccacagatgt gccaagagac tgaccgttga tcaaaaacgt gtaatcagat gtaatctgta
    44641 attgttgttt ttgctggcta tcactaaaga accatgtacc ttgatacttt gagagtcctt
    44701 ctgacttttc cgcttgtttt ttctttttca agtaaataaa acttgagaga acggtaatga
    44761 gtgaaccaag aaaatagatc atttttttta tcaccaaatc actcctttat gagaaaataa
    44821 tcgcatagac atattctttc ttctttattt cattgtaccg aataaaagct aaaatttctc
    44881 taagactttc gactggtttt tttactgttt ttctacaaat tatgatagga tacatagtag
    44941 gaagaaaggt gacataattt gaagtatcag caagtggaaa tagctttttt tattgaccgc
    45001 ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag aaataattat tgcgcatgtc
    45061 aaaaatacag gtagaggaaa agaggtcctg ataccaggcg ctgaagtggc tattgtattt
    45121 gctcctggac ctaaacggaa aaccgcctat gatctgattg ctgtaaaaaa gaagggtgac
    45181 tggtttaata tcgatagtca attgcctaat cggctggcaa ttgatggtat tttagacggc
    45241 acgatccttt taccaggatt aactggtcca tttacggtat acaagcgtga agtaacttat
    45301 cttcactcca aatttgatat ttatttagag acggtggtcg gacaaaaagc atttgttgaa
    45361 gtcaaaggta tgacacttga aaataaagcg attggtgctt ttcctgatgc gcccacactc
    45421 agaggattga aacatatagg agaattaacg tatgccgcac aagatggtta tgccgcttat
    45481 gtcttgttta ttgctcagtt cgaacatctt catctggcaa cgatccatga agaaatgcaa
    45541 ccagcactag ctgatatggt tcgacatgcg cagcaaagcg gagttcaaat cctcgcctat
    45601 aattgtcaag tatcggcaga tgaggtaact gtcaagcagg ccattccatt tgatgtagat
    45661 atcccttttg aagatcctat acaatagaaa gaaggtaaaa taatgatcaa cttaggtatt
    45721 atcggaacaa attggattac tcatcaattt gtccaagcag ctttaagcac aaaaaaatat
    45781 gaattgacag cggtatattc tagacatttg gaaaaagcac aggaatttgg ttctcattat
    45841 gaaggggata tcgaattcgc aacagatttg aagacttttt tcaatattac tcatatggac
    45901 accatttata ttgcttctcc gaatagtctt cactttagtc aagctaagca agcaattttg
    45961 gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga cgccaaaaga aatggatgaa
    46021 atcatccatt tggcaaatga acacaatgtg tatttttttg aagcagcaag aaatatccat
    46081 gaatcaggtt ttaaaaagat tgccgattac ttaccattaa aagacgagat aatcggtgca
    46141 aatttcagct atatgaaata ttcttcgcgc tatgaccaag tattagatgg agaagaaccg
    46201 aatatcttct cgactcattt ctcaggtggt gcattgatgg atttaggtgt ttaccttgta
    46261 tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc attatttccc aagaaagatt
    46321 tctactggtg tagatggtct tggatggggg atcttacgtt atgagaattt tgatgtatcg
    46381 atccagccag gtaaaatcgg tgattcttat ttaccgtcag agatttattt tgactcagga
    46441 acgctagtga tgaatggagt caatgcaatt gaacaagcac aatttcatga tcgcgcgcat
    46501 aaagaaatca ttgatttggc ggtaacagct gcagaaaatc caatggcaga tgaagcagag
    46561 gattttgcca atgtcatggc tcatccgaaa gatccagctt ggggattgtt gtatgaagag
    46621 tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg atttaagaaa aaatggcggc
    46681 atcaaattcg atgcagataa tgaggaagat ttttaatgaa cgaatttgta caagtatttc
    46741 ccgaaaattg gcagaaaaaa tatgaggata aagggtttat ctcaccttct cttattcaac
    46801 gagcagtttt tcagccacta agtaataaac aaagtatagt agcggtttct cctacaggtt
    46861 caggtaaaac actggcttat ctttggcctc tactactaaa cgtggaacca ggtgaagcaa
    46921 gtgcgttggt catttttgct tcttctcaag aactagctat tcaagtagca gatgttgcac
    46981 gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt ggtgggcgga gccaacgtca
    47041 aacgtcagat tgagggtttg aaggaaaaac cagaaatctt gattggtaca ccaggaagaa
    47101 tattggaatt gatgaaagca aaaaaaataa aagcacatca agtgaaaaca atggtatttg
    47161 atgaagcaga ccagctattt gacgctggga acagccaaat cattgatcaa attcttcatc
    47221 aagctccaac agagtatcag ctagcttttt tttcagcgac agcagatcgt tcccttgaaa
    47281 gcattgaaaa aatcactggg aaaacgtttg agacaattga cgtgacagca gaggacgatt
    47341 ctcgaaaagg actgagacat tactttttac gtgtaccgat acgaaagaaa gatgactact
    47401 tgagacgtct cacgcatata gaagattttc atggtctgat tttctttaat caactgactg
    47461 aattaggcgt aatggaagaa aaattgctgt atagaggtgt accggtagga agtctggcct
    47521 ctgatcagaa caagctactc cgaaaagcag cgctagaaca attcaaaaag aaaatggtca
    47581 gagcactttt gaccacagat gtggcggcta gaggtttaga tattacaggt cttccttatg
    47641 tagtgaatgc agaagttccg ttaagtgaag aagcttattt gcatcgttcg ggaagaactg
    47701 gtcgaatggg taatgaagga atagtaatta cactcgtaca agataataat ctaagagatt
    47761 taaaacggct tttacgtcca tcaaatataa cacttgaaga gattttttta tacggaggta
    47821 agctgcaaac tgagcagcca accaaagaag atgcagctcc tgcagtaaat aaaaagtatg
    47881 cgaaaacttt ttctggaaag gagcagaacc cagaatataa caaagaaaaa ataagaaaaa
    47941 aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc aaggcgcaaa taagcgcctt
    48001 tttatgctat gattatgaaa acgggaacaa aattggttgc cagaggaggg gttatgtgaa
    48061 agaacgtcaa gaagagatct tgcagctatt gacagaaaac caacaaggat tgacagcaag
    48121 tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca agccgatatt taagtgaact
    48181 atacaaggcc catcatattg taaaaaccgc tggccgacca gtcgtttact ctttgccaac
    48241 ggaaaagtca aaatctgatg aagtgcatgt cgactcttct acgcaagtga cttttgaaac
    48301 actcgttgga gaaaatgatt ctttaaaagt cagcatacag caggcaaaag ctgcgattct
    48361 ttatccaccg cgggggttgc atacgattat ttttggagaa actggtacag ggaagtcgat
    48421 gtttgctgaa tgtatgtatc attttgcaat cgattcagag atgctttctg cagatgcgcc
    48481 atttgtttct ttcaattgtg cggattatga tcaaaatccg caactgctct ttggacacat
    48541 ctttgggatc aaaaaagggg cttacacagg agcagctcaa gatagtcccg gtctaatagc
    48601 caaagcagac ggcggtatat tgtttttaga tgagatccac cgtttaccgc cggaagggca
    48661 ggaaatgctg tttactttca ttgataaagg tatttaccgt ccgcttggag aaagtgcaca
    48721 agtacatgaa gcatctgttc agatcattgg ggcgacgact gaatcttcag aaagtttctt
    48781 atctacgttc aatcgacgga ttcctatggc aatcaccttg ccgaatcttg cttcacgttc
    48841 actggatgag cgttatgaaa tcatctctct ttttatcaaa caagaagcga atcgtttgaa
    48901 tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc atgctttatg atgcggaagg
    48961 aaatatcggt caagtgaaac gagacttgaa actagtctgt gcaaaatctt ttttacatta
    49021 ccgtactcat catgagaaaa agctaatcat tcgaaaagaa gaactttccc ttcaagtaca
    49081 aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc gaccgattca tggacagcaa
    49141 aagtcagtat ttgacatttg aaccaggatt tgctgatgtt gtctggtcac aagatccaga
    49201 acgaaacatg caggtctata acgatattga ggaaaaagtc acttctcttt ccgaaaccgg
    49261 tgtggagaac attgatctag agaccctcat ttcaaaagat gtggatgctt atttccagac
    49321 atatgtcaaa gaattgacta aaagttcgat tcccaaagat cttttgccag atgaaatctg
    49381 gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg ttagaccgta tttataatga
    49441 aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca ttggatcgtg tgagagaagg
    49501 tcatatgatc gtccacccag atttgaataa cgtacggaaa aattttaaga gggaattcca
    49561 ggtagcaatc gatctttcaa cgatcattga agaagaagaa caagttgaga ttccttttga
    49621 tgaaatcggc tttattagta tgtttctttc tatcaatcta ggagaaagtg agcctgttaa
    49681 agaaaacaaa gtagatgttc tcatattgat gcacggcaga cagactgctt caagcatgtt
    49741 ggagatggca caagaactat taggaacaaa agtagggaaa gcttttaata tgccactggt
    49801 tatggaagtt caaacaatgt atgaagaagt gttacaatat gtcaaaaact atcaagagca
    49861 tttgacgaat ggcctactct tgttaacaga tatgggatca ttaaacacct tcagtgattt
    49921 gatttatgaa gagactggca ttcggacgaa agctatcacg atgacaagta ccatgattgt
    49981 gatagaagcg atacggatgg caagtgttgg aagaagctta gaagatattt atcaaaatat
    50041 ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa gcggaaaagt ctgaaacttt
    50101 acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt gaaggagtag ctgccaaatt
    50161 atatcagcgt attatgccag tgattaatca aaataaggta gaactgatcc agatgcagtt
    50221 tttagaacgt gaatcattca aaaaacacat cgatgcctta ttggaagaat atgagatcaa
    50281 agcaattgcg ggaacagttg atgttgatta tcaaaatatc ccattttttt cagcctatga
    50341 cgtttttgat gatgaaaaac taaatatttt aaagcgtata gctgatgatg agatcccgac
    50401 agctaaaatt gtcgattcac ttgctggatc attaagcgat gtcttttcta ttgatgaact
    50461 gatccgttca ttgcaaaaga cagttcagca gatccagacc gatttgtcga ttatcgtgga
    50521 acctggagta gatactggaa tcgttatcca tttagccttc atgttagacg gattgatcaa
    50581 acaagaaagc catcggcaat ttgagaaact gtctattttc tcaaaaattt atcgtttgga
    50641 aatggatatc gttcgggcaa acttgatggc actagaacga aaatatcagg taaaaattcc
    50701 agaagatgat atcgcatttc ttacacaaat gtttcttgaa aataaaataa aaaaatgaac
    50761 acactaatca tactgtttac acactaaaca tctgtgtgcg cactataaaa aagagctaaa
    50821 aagcatgaaa acgctatttc agctcttttt tttattggca tgcttcttgc gttatataaa
    50881 agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa aacaatcatg ttagtttgtt
    50941 cagcagggat gagtacgagt ttattggtca caaaaatgca aaaagcagca gaagaaaaag
    51001 ggatggaagc cgatattttt gcggtctctg cctcagatgc agataataat ttagaatcta
    51061 agaatgtcga tgtattatta ttaggtcctc aagtacgttt catgaaagct caatttgaac
    51121 aaaaacttgc gcctaaaggt attccattag atgtcatcaa tatgcaagac tatggcatga
    51181 tgaacggaga aaaagttctt gcacaagcag aaaatctaat gaaataaatc aagtatgcag
    51241 ttgggggtat aacaacgtgg aagatcaaca aaatctagaa gccatcatgg ggttaatcat
    51301 gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt gcggctgcta aaatcggtga
    51361 ttttgagtta gcggatagga aaatcgctga tgcagaagaa tcacttatac aagcgcatca
    51421 tgcacaaaca gacatgttga cacaagaagc acaaggaaat catatgcaag tgacattatt
    51481 gactgttcac agtcaagatc acttaatgac atctattgcg tttactgatt tagcaaaaga
    51541 aatcatcgaa ctttatcgtc gcgtatacag cgaataaata caacttcttt tttgaacgga
    51601 agaagaaaaa tttttgaacg gattttttat tttgttataa ggagaactct ctttatataa
    51661 aaaacttata ggaggaagta aaaaatgaac ggactatcaa gttggatgga gaaatacatc
    51721 ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag ttgctttgcg tgatgctttt
    51781 atcggcatac tgcccgcaac aatggcggga tcagtcgctg taatgatcaa tgcaattatt
    51841 cgtgatttac cgcaacagtt taacagtacc tatgctgaca gtttagcagc agacaaagga
    51901 atctttgccg tattgaatgt catcatcgga atcaatggtt ttgtctggaa cggtacacta
    51961 gcaatcgccg gattgatctt tgccttttct tgggggtata atcttgcccg agcttatgga
    52021 gtaaatgatc tagctggggg gatcgttggt ttagctacct tgattcaagg tatttccttt
    52081 ggtccagcat tatcagcagc attagatgtg aatgttcctt caaatgtcgc agatgcaatc
    52141 aatagttctg agattggtgc aacgattgca gatggcacac tgtcagttgg attatggggt
    52201 tggctaaaac tagatcattt gaatggaaac gcttatttta cagttatgat catgggagct
    52261 atttcagtca ttattttttg taaattgatg cttgccaaca ttacgatcaa aatgcctgat
    52321 tcagtaccac cagcagtatc taaagcattt gcagccattt taccagcgac gatcgcttta
    52381 tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag ctgatgggca actgatcatt
    52441 gatttggtgc aaaaatatat tgcagaacca ttcctaggat tatctcaagg gttgggagca
    52501 gtattgatcg tcacagtatt tgttcaagta ttctggttct ttggtattca tggatcaaat
    52561 gtattggcac cagtgctaga gggaatctgg ggacaagcac aactaatcaa tattgatatt
    52621 ttccaaaaag gttataacgg aaaaactggt acaccagccg tgttagatgc aatcgaagat
    52681 ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt ttgcatggtt tggcggatca
    52741 ggcggaacga tcgtcttact gatagctatc ttgattttct ctaaacgtgc agattatcta
    52801 acagtagcta aattatccat tgggcctggt atcttcaata tcaacgaacc agtgatgttt
    52861 ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat tcttgattgc tccagtagta
    52921 gcaacagcaa ttggatggac agctacttat cttggcctag tcgctccggt atcgcagcaa
    52981 gtcacatggg tagtaccgcc gttcttattg tcattcttag caacaggagc ggattggcgg
    53041 gcaccgatcg taacattagt atgtatggtc gtgactttct tgatttgggc accatttgtc
    53101 atctctgcaa acaaaatgga ccctgccgca ggcgaacaac aataagatga acgtgaggag
    53161 cgggacgctt taagtttcgt tcctcataat gaattttcga ggaggaactg aaatgattac
    53221 aattgccagt gcaatgcaac aagatgaaat caaagaattg ttagaaggat acaatgaaga
    53281 aggaatgact tttacattta aagaaaaaca aggaatcaag ctgttctttg aaacaagtgc
    53341 agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta atcaaagcac agccttgggg
    53401 aacagtcctt tattttcaat caacagctgt ttagaaaatg gtttaaaaag ataggatggt
    53461 tgagatgata aaaaacggtt tatttttatg tagtattggt tctttgatcg tgttgtatag
    53521 tttgaatcca cattcgcaaa ggtatgattt gtatagtatg gtcacaggtc tttttctaac
    53581 aggattagga atctatttct tcatgaaggg gaaaaaaaga gaagaagcaa aggagaaaaa
    53641 taactaatgg aaatcacgaa aaaattaaat attccagcat cgttttttta cgataaagtt
    53701 atggactctg tccttttcga tgtacgtaaa gcgaccggca aatcagtaac tagaaaacag
    53761 ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata accgggctcg cataaaaatc
    53821 gaagaagtca ttgaaaacca gtcttataag tttcgtacat cgacaacgaa gaatgaattc
    53881 gtcgttcatt atcaaatcaa accattggac gattcgacct gtgaggttca ttacacagaa
    53941 aaaatggagt cttatggcat gcttcaaaag ttgaatgata tgcttttagg aacaatcttg
    54001 atgtatttta aacggagacg tttcaaaaaa atgctagata tgatcgaagc aacgtattaa
    54061 atcaatgaaa catggtacta tttagttatt gaaaaatgaa gtaagctaga aggaattttt
    54121 cataggaaga tagatctttc ctgcgaatcc tcactagctt ttttgtagga aggggcagca
    54181 aaatgacaaa caaagtaaga gagctaggtg tatcttcgat tgaggaaatg tttgatttag
    54241 ccacttatgc atttaatggg gcagatacac ctgaaaggcg agaacgtttt cgtacacttg
    54301 cggagaattc ttggaattac ggattttttg atgaggagga tcggctaaca agtcaagtca
    54361 tggcgacccc ttttccagtg aatttttacg gacaagagta cctaatggcc ggcattggct
    54421 atgttgcttc ttatccagaa gcaaggggac aaggcgggat caatcagatc atggaaaaga
    54481 tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta tctggcgcca ttctcctatc
    54541 ccttctatcg gcgctatggc tatgaacaat catttgataa aatcagttat caactaaaca
    54601 gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat aaagcgtatg gattttgaag
    54661 atgcaaaagc tgcaatcaga agaatttatg aacagatgcc ggaaaaccaa cgagctggtc
    54721 tgaagcgcga agactggtgg tatacgtata aattcaagca aaaaggcaat taccagtttg
    54781 ctttgcattt tgatgataat gaggaagcaa cgggatatat cgtttatcag cttgcaacgt
    54841 cttcctttat tattgcggag tggggatttt tggatcatga tgctttttgc agccttgttc
    54901 gcttcgtaag ttctcacaat ggtgcttttg aaacatttga atattcatgc ggctacggcg
    54961 gtaatgatca aaattatcta ttagaaaatc catttgcttc tgtaatgatc acaccttata
    55021 tgatggcgag gatcgtagat atttctttgt ttttggaaaa ttatccattt aaaaagagac
    55081 aagctgcatt tgccctacaa atagaagaag atcattatgc gaaatggaat gaaggaatcg
    55141 tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa agtagaacaa acaaaactcc
    55201 caatcgttcg aggaagtatc caacatatca cgcagctatt attagggtat cgtaaagtgg
    55261 aagagttggt attccaagaa agaatacagg tgacaaaaga atatgaagaa acgttaagct
    55321 caatcttgcc acagcaacag ccaattctta atgactattt ttgataaaaa acgggtgatg
    55381 acaatatttt tgtcaccacc cttttgtttt tcatttggca tttgaaggaa aatgttaaag
    55441 taaatcctct tttttcgtaa atttgaattt tttgttttct tttgttttag ggttttcttt
    55501 tttgtggtct tggattgctt ctttcgtaat cttttcccga ttttttttag atgaatctaa
    55561 gcgttctgcg tatacttttt cttttggctt atcattcatt ggaaaggacc tccctacatt
    55621 atttcattct acctctagtt tatcatctgt taaggactag tggaaacgtt ttgcctaact
    55681 tataagctga tttttttagc agataagctt gcaagtaaaa aacaaacaag ctacaattga
    55741 tttattgaaa ttgttcctgt agctcagtag gatagagcaa tcgcctccta agcgataggt
    55801 cgctggttca attccagtca ggaacgtata aataacccct gtatcctttg cggtacaggg
    55861 gtttgttttg tatttgtgcc aaaaatgtgc caaactagtt atcattttta tataaactat
    55921 cgaacacagt attgattttt tctttatcct gatcttcaag ctctcgaaca atatgaagat
    55981 aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat
    56041 ttactccttg ataaagtaaa atagatgcat gtgtatgcct tagtccatgc aaagtaaatt
    56101 gcttttcaat ccctaattct tttaattttt tgcgcaaata tttgctgacg gcgtttgatg
    56161 aaactaatcc attttttaag ttgaaaaaaa caaaattatg agggtttttg atttcaaaat
    56221 tctcgaataa ttctttttga ttgattttaa acttttttaa gagatcaatc gtatgtttat
    56281 caatggaaat tttcctattt gaagtttcgt tttttgtttt tccccattca tttaatttat
    56341 aattccatgt tttattaata gtgatgattt gatcttcgaa atcgatatct tcccatgtta
    56401 atcctagaag tccagcaaat cgcattccag tggcaccagc aactaacacg agcataggag
    56461 aagagtactg cgcagaaagg ttttcttcta cgagtttcat taaagattta aattgatcat
    56521 aatctaaata tttatcttct tttggcttca atgaatcttt tcctttgatt actgcttttc
    56581 tagttggatc aaacggaatt aatccttctt ctacggcatc tttcaacgac gctctaatat
    56641 ggttattaaa cttaataacg gtagatttta catggtcttt agcatactta tttaaaaagc
    56701 gttgatatcc cactctatct aaatcagaaa tcaataccgc tggcatatat ttctttatgt
    56761 tcattaacgt atcttcgtat tttctataag tgataggaga tactgttcct tctttataaa
    56821 gttgcatcca atcctcaaag taatctgata ataatagatt tttgcgatcc cctttaaggc
    56881 ctttgttcaa gttatattct aattcgttgg cggcatcttt tgcttctcct tttgtcttaa
    56941 atcctgattt tcttatcttc gtgtattttc cgtcatcttt tttgtatgaa atttcgtatt
    57001 gccaactatt tccacgtttg actaatcttg ccataattga atcagctctc tttctctgat
    57061 acaataggaa ctataaagaa gcccattgtt tgggttttat tattttttgc acatcctttt
    57121 tgcttgccgg cgcaaggatg tgctatttgt cgttttaatg gtataattgg atgtgtatta
    57181 gatcatccaa tcagaggtgt ttattatgat taaagtgatt tttaaaagaa taactgaact
    57241 tccagagttt agattatttg ttgattattg gtatgtctgg ttttttattg ttgcattgct
    57301 gataggtcta gatgtcatat tagaaagaaa acttaagtaa gagttgagtt aataaattga
    57361 attcctaaat gagatatttt gtagcccata ttttgtttgc cgacatcacc taatactatg
    57421 gagctaatcg ttttttctaa cagaccaaaa tcagataatc tatttaagct accatctatt
    57481 agctcttgtg aatagccaga catgctgata tttccagtga aattttcgtt tccacgactc
    57541 attaagcatg ctaacaattg aatctcaatc gatgttaatt gattcagtac aagaagataa
    57601 tactcctcgt tatcccatga agcgttgtta attcgaagta aacaattctt ataaagattt
    57661 ttatacatat ctattttatt ttgtgccttt gatttttcta cctcgtcatt tatcccttcg
    57721 aatattccaa tgagttggtc cttgttctct aagttgaaaa catcttctgg aatttgatcg
    57781 cgaatgtttt ctaatgattc gtttaaattt ttgtagaatt tctcgatgcg tttgaaacgc
    57841 ttctcgtttt tagacccaaa ataaagtgac tgcacaacgc caccaatata aggaattgct
    57901 ccaataccgc cttcaactat taattcaaga tattcttttt tggttagttt ttcattgcta
    57961 ttatccatag ttacctccta ttttgtcatt tcctttacaa gcattgtgac tcctgatggc
    58021 actcttttgt ataggtttta ttattgtgca caatctaact cttagtgggg cgtggttcat
    58081 ttatttttta cttactttac tattaatcat atcccattga gcataggatt tttctatgtt
    58141 cgatcctctt gaactaattt ctcctgtaaa agttacttta tcgccctttg taaaggtatc
    58201 agcattgttt aagtccttcg caaaaattac atatgaaact ttaggtgttg cagaaatatc
    58261 attccaactt ttatcatcgt actctttgtc tgctataatg gctattctag tagtcattga
    58321 ttcgataaca gttccttcaa aagtatatgt tttaccacta atatatttag taaaagctgt
    58381 attttgctca gtagctggag taatagaata ataactatct aagaaagcac cccagtcatg
    58441 attatttgtc tctaaaaatt cttcaaattg agttaggctt ttattgctgc tgttagatgt
    58501 agtagtttga gtatcagatg atgagctttc ctgtttgtta ttgtcatttg cctcgtttga
    58561 tgaggaaagc gagaaaccta cggtagtaaa aataatcccg ataataagga gaatcatagg
    58621 tgttttctta tttgtttttt tgataaatct tacaattaac atgattaaac ctataataaa
    58681 taaaacaata ccagtaaaac caaatatacc gtccattaat agatcctctt tctcttttga
    58741 tattgaatat tataaaccaa gcaattcttt tttcttaaaa tcgaattctt cttgattaat
    58801 aatcccttca tctaacaact ctttatactt tttaatttca tctgcacttg aaatttgtgt
    58861 ttctttggaa ttattatcta atatagattg ttgcttctct atatattttt ttaaattttc
    58921 catttcattc caatattttt ttgtaaacat aacggtgttt tcatctttcg tggccgctaa
    58981 tatacctccg cgactttcgt ttccgcctag caaggtaaat tgtatataac cattactcat
    59041 acctggtttt tttaattgta cggctgatat atttttaaag gggatagttt tttctccttt
    59101 aataccttga ttaactaaat ttataaatcc ttttctaata attgaaatag aatcatcagt
    59161 tactttaatc aaggtttttc ctggagattt tattaaaatc tctttttcca ttttttcttc
    59221 ctcatttctg tgatatgatt ttttttgtag aagctcatat atgaggaaag agcccgtgtt
    59281 gcagcacggg cttttttaat gttttgctga aatcggtttt tttaataatt cttgatgtat
    59341 tcatacatat tgccttgcgt aagaatattt tttcttaaaa tagcattggc agacagcata
    59401 acaagtgtat ctgagcttat tctgaataga ataatactcc ataaattttt cgagattaaa
    59461 ctgagattca tcagtcagtt cgttctcaat atagatattt aataaaatta gaatagctat
    59521 tttatctgct tctgtttcaa attttgagtg aaaagttgta gaagtatcgt acaaaactga
    59581 aaattcaaaa tgagaggcgc tgaaatgtgc aagttcatga gataaatgaa aggcttctgc
    59641 agtttcaccg tatagatttt cattcaaaaa aatgattctg ggttttggat agtagaaacc
    59701 aggctctttc atctccatat aaactacttt caaattgtat tcgctcaaca tttctttcaa
    59761 tttcaaatac atacaaacca tcactccaac tattcatttt cctctaaagc tttagcaatt
    59821 gcaatcgctt tacgcattgt ctccttagat atttcttttc catcaaaaga aaaaacagta
    59881 tcgtcttctg ataaatccac atgtttaggg gtttctcttt cttctctacc tagaaggtag
    59941 tctacagaga catcgaaata gtcagcaatc cgacttaatt catctgaatt aggagtatta
    60001 tttttccact ttgctagata accgttagaa tatccaaaat taatttctaa ctgccgtata
    60061 gatatctttt tcctcttcgc caagtctttt attatttcat aggtattcat cgataaaaca
    60121 acctttctaa atgtttacaa aaaaagttta gaaaaatacg cagaaataat ttgactaatt
    60181 ctgagtaatg agctatacta tatcttgtaa acagatttaa caactaaaaa gacaacaaaa
    60241 aacactattg atatgtaaat gcagaccgcc aagaaagctt taaaatcaat gtttttatgt
    60301 cttatttaat tatgctctta gtatagagta ttactcagat actgtcaatt gaatttagaa
    60361 aaaagttgtt aaatttgttt acgaatataa aagaaaggaa tgagaaatat gaacacaccg
    60421 caaattttta atttcgaaca aaacgaagtt cggacaattt tagtaaatga tgaaccatat
    60481 tttgtaggca aagacgttgc gagtgttttg ggttattcaa acactaaaga tgctttgtcg
    60541 cggcatgtag atttggaaga taagatgggg tcgcgaatca cgacctcagg tcaatcaaga
    60601 gagatgacaa tcatcaacga atctggtttg tacagtttaa tcttaaaatc aaaacttccc
    60661 tctgccaaaa aatttaaacg ttgggtaaca agtgaagtgt tgccagcaat tagaaaacat
    60721 ggaggttatc taactccaga aaaagtagaa gaagctttgc ttaatccaga tacaatcatt
    60781 caattagcaa ctcaactaaa agaagaaaga actggaagat taatcgcaga acaaaagatt
    60841 gcagagtacg aacctaaaat ctcctattta gatagcatat tatcttctac agattcagta
    60901 acaattagtc agattgcagc agattatggg atgtctccac aacagatgaa taaattactt
    60961 cataaactag gtattcagaa aaaagtcggt aaccaatggt tattgtgcaa aaaacacatg
    61021 aaccaaggat acacaaaatc tcatacaact gagatcccga aagccgatgg tggcactaaa
    61081 attgtaatga ataccaaatg gacacagaaa gggcgtctat ttatctacga attactaaaa
    61141 aaagaaggat attaccctca aatggattta gaggaaattg gttagaaagg agttttagta
    61201 tgactgacat tgcagaaatc actcaacgag atagagaaaa aatcaaagaa tatgtcgaaa
    61261 gttcaaagtt cttaacttac accatgcttg ctgaaagatt tggaattagc aaaagctact
    61321 tatctttaat tttaaacggt aaaaagactt ctgcagaagc aaacagaatt atagattcga
    61381 ttatcactat gtacgaattg taaagggagg aacagctaat gcaatatcta gaagcaaaaa
    61441 tcccaattcc agaaggctat gtaattatct cccaagtgga ttatgaggag ttaaaaaaag
    61501 ctgatgatac tggtagatgg atgacgttgc cagaagtgct agaacggatt aacagaaaat
    61561 atgattggtt tacttctaga gttttaaaga acccaagata tagaaatatt atcgatatcg
    61621 agaaaaacaa aaatggattt gtctattatc cagttgaagg aagagacaca tatctatttt
    61681 taagaagtaa aacacttgaa tttttagaaa caaatttttc ggaaatctta aggaggcaag
    61741 cggatgggaa aatttaatag agcgctagtg ttcagcgcac cgctaatcat ctacgcttta
    61801 ggactttggg gaagcaggca agcgttgata ggaacgattg tttacatggt ttggattttt
    61861 atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga gggggctgac
    61921 taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt ttaattcttt
    61981 cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt ggatttgggt
    62041 tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa aattaatttt
    62101 ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat ttagcggtac
    62161 agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta atttcttaaa
    62221 tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat taactaacat
    62281 agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat ctgcagcgaa
    62341 gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt ttatccctgt
    62401 taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg ttaagtgaaa
    62461 aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg agagaagaaa
    62521 taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc ttacaagtgg
    62581 cagaaagctt cgagaaaaaa catcaacatg ttttaagaga tattgatgca ctaaaaaaag
    62641 atgtgtccaa ttttggacag atgtttgtag aaggtaatga accagattca tatggcagaa
    62701 atcgacgagt tttcttcatt agtagagatg gatttttctt gctggctatg gggtttacag
    62761 gaaagaaagc tatctacttc aaacaaaaat acattgaagc attcaacgaa atggaagatg
    62821 ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat gatgatctat caattagaag
    62881 aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga attagcggac
    62941 aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt ctagggggaa
    63001 aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat
    63061 ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg
    63121 ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa attgatcaac
    63181 tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc gctaagaata
    63241 gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct aagccaacaa
    63301 catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag
    63361 agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc
    63421 tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa acaagtggtc
    63481 gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa gatgatttag
    63541 gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag cattcgaata
    63601 aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa gagtacgaca
    63661 gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt gctgaaatgg
    63721 aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt
    63781 aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg
    63841 tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtacaaacac
    63901 tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt
    63961 atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc
    64021 gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa
    64081 atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta
    64141 tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa gaaattaaac
    64201 atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc
    64261 ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa
    64321 actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag
    64381 agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag
    64441 cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag gaagtgaaca
    64501 ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt gaaattcgtt
    64561 ttattgatcc tagacgtttc accgtacagc aacgaaactt catctatgcg ctgataggcg
    64621 atatttttat cgatacaggc atgccaacgg acttctggaa ggaattcttc tactttcgtt
    64681 ttgaaggtgt cacagggcgc aaaataagcc tgaaagacga atcgaataca actgtgagtg
    64741 atgccaatgt cttagcaaat atcatcttag atttcatctt tgaacatcat attcctttca
    64801 aagaaggcta tgagatttta cctgcgaatc aagaatatta cttctacaaa tgcatcacaa
    64861 aaagagtctg ctgcatctgt ggcaaaacag gagctgacat cgatcacttt gacaaagcgc
    64921 taggaagacg aaagcgcaaa gaagttgatc atgcagagta cacatttgca gcactctgca
    64981 gaatccatca cacagagaag cacaaaatag gtgtgattaa tttcaaaaat aaatatcaaa
    65041 tcaaagggat caaattaaac cagaaaacaa tcaaaaagtt aaatattgga ggggaaaaat
    65101 gacagaacat cgaagttatt atgcgattat accagccaac gtaaggtacg acaaaagact
    65161 taaaccaaat actaagttgt tatacggaga gataacggcc ttgtgtaatg aaagaggctt
    65221 ttgttgggca ggcaatgagt actttgcaga tttatatggt gtgaataaag agaccatatc
    65281 gcgatgggta agtgatttga ttaagtttgg atacttgaat cgggaaatca tttacaaaga
    65341 gggtaccaat caaataatca ataggtacct acgaattaat caatacccta ttgacgaaaa
    65401 acgcaatacc cctattgacg aaaaagtcaa agataataat acatctatta ataatacatt
    65461 taataataca aaagaatata taagagagtt accgccttcg aaaaaatcga aggctaagcc
    65521 cgtccgtcat aaatacggag agtataaaaa tgttcttttg tcagatgagc agatggagaa
    65581 actcaaaaca gaattcccta atgattacca agagcgaatc gaacgactgt cagagtattg
    65641 tgaatcatct ggtaagactt ataaaaacta tttggcaact attcgaagtt gggcaaggaa
    65701 agaaaaaagt gaacctaaga acgcaagcag tggatacaag cgcacaggaa gacgagagaa
    65761 gcttcctgaa tgggcaatcg accaagaagc ctatctcaag aaaaaagcgc tagaacgagc
    65821 taatagacaa tcaaaagcac cattctaaga ggtggaaaaa tgaagatcga ttatctagaa
    65881 ctaattaatg aaatagcgaa ttataaaaag ggcgaggaat tagacgtcct gagagacgta
    65941 tatgatcaac tcgaagaagc tggaattgaa ggaattaaga atgatcgttc gagttggagt
    66001 aaactcagat actatttcgc actctatatc gatggaacgc aattaagaaa tttagcatat
    66061 accaaattac tattcattga ttgtgttaaa ggcttgcaaa aacatcttaa tgaacttgag
    66121 caggtgtaaa caagatggac ctaaagacat ttacagcaca gatcgaatta atgcatcaag
    66181 aagctttaag acaaagtgtg tcgtacgaag acaagtggct caacacattt catggcggac
    66241 gtgagagcgc acttgatcaa gtactcaaat tactgaaagg agaatgtcgg gatggataag
    66301 aaagcggcaa tgcagcgaat tatcgaattg acttattcag aagattggca ggaagataaa
    66361 aaaatagttg cagaagtcca aaagctcggt aaatcaatgt ggactgaaaa gcccaaacgg
    66421 agaacgccga gaaagattgc aatctggcat ggtgatcgaa ttctagtaac gggtaccgct
    66481 aaacagttat ctgaaattac tggtctgagc aaaaatatta tttgggatag agctaggagc
    66541 ttatggattg attcaaaagg aagacagttt aggtatgtgg aggagaaata atggacgaac
    66601 taatcgcgaa agtagagcag tgggcaaaag ataagggatt ggatcaagcg gatttcagca
    66661 agcaaatgtt aaaaacggtc gaagaaatcg gggaagttgc cgcttctcta gctagaaaag
    66721 atgaacatgg tttaagagac ggaattggcg atgtagtagt aaccttgatc atcttggcaa
    66781 tgcaaaatga tatggattta tacgagtgct tggattttgc ttatgacgaa atcaaaggtc
    66841 ggactggaaa gatggtcaat ggtgttttcg ttaagtccag tgacctgaaa taattccatt
    66901 aatggaagaa ataactgaat aggaggatag acatgattga tgcaaaacaa gtgcaaaaac
    66961 agaaagatgg aatgttgatg tttgaagcat atgttctacc atttcttaat caatttgaag
    67021 ttttggaatg ctctgccagc ggagaagaat tggagtatgt tgtaattcgt gaaaccaaag
    67081 aaaacgttca gaagctaaac gaatttcttt gcactattaa ctgttgggat atgatagctc
    67141 caggattttt atgtccagct atgggagaat ttttggaata ttgtcgtctt gaagatgcag
    67201 gcactttgga tttagcttat ctggtataca actacctaaa catcaacaca gatcatttgt
    67261 ggtttggcac tgccgaaaga aaatgggtag tccgctaacc accaatatca atgaaaaaag
    67321 gcggtttcct gaaagtggaa ttgccgccat tgctgtaagg ttctttacgc gactcaaggt
    67381 cacagaaata taaatgcaaa tctcttttga agtgatccaa agctctcgga aaatggaagt
    67441 tttttgtttc aaagaataaa tgagatttgc gtcaagaacc tgagagttat ttccaaaaaa
    67501 gcttccaaag aaccgaatga gctaaggaca tcatgtgtaa acacataata tagctctcct
    67561 ttcacggcta atcataacat gaaaaatatt cgatgtcagt aggaaaaaag gtgaattatg
    67621 aaaagaatat taaattatcc cggcagtaac agattctgca attaaactcc gaagctgatg
    67681 tatcagtatc aactgtagaa tattatgagc gtggagaaag tcaagcagtc acaaatgtaa
    67741 aaatcgataa cgtcaacggt cttatctgtt tagatgaagg cgatagtatg aatgaaattg
    67801 ctgcagacca tgaattatca gaaactgctt taattgatca aatcgaatta gctagacaat
    67861 actttgttcc atatgcagat acctggtcca agcataagaa tgacatcatt tttccagagg
    67921 aacaatgaga gcaactgatc cagtaattat ccttgaggaa gccaaattta tttggactca
    67981 cgaagagata gagcaagcac gcttgctctt ttctcaagga gttaagccga gcaaagtagc
    68041 tgaaataatg ggtcaaaaga ttcttgatgt cggattgctt ttgctccatc tagcagaaaa
    68101 aatttgattt gaggtgaaaa tgatgattca acttgcaggc atccaaacag ggaaaattta
    68161 tttttctgga gaaagcaaaa gcgaagccaa ccaatggcta ttgaaaactt acacgaacaa
    68221 taagaagcta aggaaaaaat atcctgattt gttgctaaag gatgatcaga ttatgccgga
    68281 accgatgatt ttgacccgta aggagggaag ttgaaactaa aaaaccagcc gtgtgggctg
    68341 gagttgctat ttctttttaa taatgagttt ttctaaattt aattcattct cacgagtttg
    68401 cgtccaataa tcagaataat attttaattg tttaagagta ggaatgtcgt cttcggtaaa
    68461 attagaaaat aattctggag aattatcttt ttttaattgt tctaatcctg attctttaaa
    68521 acgttgttca acgagtttta ctaaattgtt gatattcatg ataaatcacc tttttaaatt
    68581 taatttcgat ttgattaatc gatacaagta ttatatcaaa taaaaaagct gatttagggg
    68641 gtcttacaaa aacactaaaa ttttttcaaa aagaggagga acggtggtga aagtcagaga
    68701 acaattcaag atagtgagcg acgaaggtaa tgtttacaaa ataaagatcg ttaatattaa
    68761 tgaatatcga gaagcagatc acaaatattt ggttgactta tacatgaacg ataaatattt
    68821 gggagaagga ttttgtggag attcactttt tgatgatgag cgaattttga aggaggaaac
    68881 agaatgatcc acgaactaaa aatactacca gaatattttg aagcagtgac gagcggacgc
    68941 aaacaattcg agatccgcaa gaatgatcgt gacttcaaag ttggtgatca gttaatacta
    69001 cgtgaatata aaaaatacat taaacatgaa ttacctggac taacgagacc aacatttagt
    69061 tacactggtg actcatacaa agcagagatc acttatatta ctgattacat gcagaagtcc
    69121 ggatatgtag tgttggggat taaaggggtg aattaaatga aagtatacat cttagctatc
    69181 acagagggca catggatgtt cccagtcggt tccggaaaaa tctataaatc aaaaacagct
    69241 gcttataaag catttgaaaa atacaaaaaa gaaaacggtg gcggcactaa cgcaaagatt
    69301 ctagtggctg ataactggca tgaagaaggt gaacgtaatt gatttacctg ttcattttcg
    69361 tggtactaac tatttttatg ctagtaatct tattcaaaaa aagatgagta aagacgaacg
    69421 aaaaattctg gccaactttt atttagtatt aaaaatcgaa caaaatgtgt tttcggaaag
    69481 gagaattgta tgggaaaaac aaaatcaaag attaagaaga aaaaacgacg tttggaacaa
    69541 aaggcaatcc agaatggaac ggctaagaaa aaataaaaaa gctacccctt tcgagatagc
    69601 agcgcaatac tattctacca taaggggtgg cgtttgtgag atttcattgg ttaaaagatt
    69661 accaagaact agatgagcag attctttact taaagtggaa tcttaataaa agtaagcttg
    69721 aattgaatcg atgggtctgt ggtgatttag caaacgtccg catcgaaaag aattcgagaa
    69781 cttcttcttt agaagagaat atccaaaaga tagaaaatga attggaattg ttgatcgagc
    69841 aaaaagaaga aatgttattg ctgatagata gcttttctgg tatcgataat caaattgtta
    69901 agatgaaata tgttgatcaa atgagcttag aagatattgc tgaatctgtt gggtatagct
    69961 catcgtatgt cagacagcga catgcagaaa ttagaaagac attgaacttt ttagatgagt
    70021 atgaacacag acaagctgac cgattgaaaa aagaaaacga aattgatttt tacaatagcg
    70081 agaagtacaa agaacaactc tctttattct aaaattacac tgttcactaa atgttcggtc
    70141 attgtagcta tgtaaacatt gttagcagca tgatattcta ttagtgtcaa aaaaatatga
    70201 aagagccaag atatcccaac cgttttatta attggtatct gtggctcttt tattctttta
    70261 attagtagtt ggcataaagt tatttccatt tataaattga tcagctaaat cagcatcata
    70321 atcagccgcg ataaattgcg ctgctgattt aataaatgca tccatatcat gaacatcttt
    70381 attagtatgt ctacgaacat agtgcgtttc atcattacca atccaagtag cagctttggc
    70441 taatctttgg agtttaggaa aatcattcaa ataattttca attgttttac ctaataacat
    70501 tttttttatt ttatcttcat cctctgaatt tttagatatt gcataatctt taattagaaa
    70561 ttctagagct tttctataac caactccagc aatttgttcc aattgctcag atttagcttg
    70621 tgtggcttga gtataaatat caacaaatgt aggagaaact ttttcaatat tttctggtaa
    70681 atctatttta atcggcggac ggtaataatc tttaattaaa atattttcag aatatccttc
    70741 atcttcatta aacgtattta taaattgtaa actataaaat ttgttgcact catcatttat
    70801 acactgtact aatacgccaa agattctatc attatcgaac gcactatatg atgaaacccc
    70861 aaaatacaca tacggagtca ttatatttcc gcaatgcgga caactatttg gcaaataaat
    70921 tgtctctgta ttttgacgat cacgatactt aatattaact tcttgaagca ttaaaacacc
    70981 tcgcatcttt tttataaatc ataccacaaa atatctgata ggagagtaaa caaatgaatg
    71041 attttcatga agcggtactt agtattgaag tagaatcaag tttggctaaa gtttataaga
    71101 aagcaattga agcagagaat agtccgtaca gagaaaattg gaatggtaat catgcacatg
    71161 tgcaagtgga gaatgatgat tacagaactg gaatgaatac gttagttatt tctttattgt
    71221 cacacactct acccaatctg caggaaacga ttgaatggta tgagcgaatg ggcgcaaaag
    71281 taatccgtac aaattacaaa ggagagaatt aaaatggcaa tgatcaaaat tagaacatcc
    71341 attagtggaa cagagtattg ggattcagaa aagaaaagga cagtagttgt tccgaaagat
    71401 caagaaccaa attttgaagt aaaagaagaa aaaggaattt tgagtgatgg caaatcattt
    71461 gttgctgtca atggcgaact catagctcat agcagtgaaa ttcttgatag cgatggaaac
    71521 actgctgcta attttgatgg agacgaagct actaatgatc agtccactga agatacggat
    71581 gaactggaca atatgactgc aaaagaattg cgtgcatatg ctaagaaaca tggtattgat
    71641 attcctggtg ctatccgtgc aaaaggcgat atcataaatc ttattcgtga agtagaatga
    71701 aatactgtca gtttgacgga tgcacgaaca aaatagcaaa gggtatctac tgtactgaac
    71761 acaagagatc aagcagatca cgcaagaaga agcagcaagc aaagtctgtt tatcatcatg
    71821 agaacaaacc attctatcga acgcaagcat ggaaagatat gcgtcaattt atttatgaaa
    71881 gagaaggtgg tcattgtcag cggtgtggtc agttcatatt tggaaagaga gcacacgtcc
    71941 atcacattgt accaatcaaa gacaacgaac tgcttaagct tgatccaaac aatctcatgc
    72001 tattatgttc aaaatgtcat ccaattgttg aaaacgaaac ggaagacaaa aaagtttttc
    72061 cttcgtattt caattgaagc ccccctattc attttcaaaa ttttttcgcg tggggagata
    72121 gggtagcggg gagtcacgcg catcgttagg tcaaattttt caaaaaacaa aggggggtgt
    72181 atacaaaaat gacgactaaa gcgcaacgta aagcgattat tgatgaaaaa gtaagtgctg
    72241 aaaaagctcg tatcttagaa ataatgaatt tgtctgattt gtacaccatc actcttgatc
    72301 cattaatcga atcatacttg gatatttttg aaatttacca acacaaatat ttattgtgga
    72361 aggaaaaagg atttccggag acgcaaaaat tcacgaataa atcaggagct actaatcaat
    72421 caaaacatcc attggcacaa caagtagaaa cttgggcaga taagaagatg aaagctctag
    72481 atttattagg attaacaaac aaagcaaaaa ctggtagact aattgctgga ggatcgaccg
    72541 caagaaaaga tgaagaaatt acacgcccag aagtaaagcc agtagatgaa ctagcagcgc
    72601 acagaaataa atggcgtaag aaggcaggtg ctgaaaaatg attgaacctg gtgtaaatta
    72661 tgctgattta tttgcaaaag aagtaagaaa gaaacctaag aagtatccta agaccgttcg
    72721 tttagcaata gatcgctggt atcagtggaa aaaaagaaaa gatatttggt ttgatgtaga
    72781 tcgcgcgaat gaaatgatgg attgggtaga atcatttatt gttcacacaa aaggagaaat
    72841 ggtgggaaaa ccgtttcttt tagaaccctg ggaaaaattt atttattctt ggatttatgg
    72901 ttgggttaaa gaaaatgaaa aaggccaaat tgtacgcgtt actcgtgagg cttatgtcca
    72961 gattccaaaa aagaatggta aaacattgat tgctgtcggt tcattaggat atgcaatgta
    73021 cggagaaggg gcgttatcag ttgattgcta tgcttgcgca tctgattttg ctcaagcgca
    73081 atatgctgcc aaaccttttg cagttacaat cttaaacaat ccaatcctac ttgaaggaac
    73141 taaaatattt agggggccaa agggaactgt ttctagtatt acttatgact atattcatga
    73201 agatatggct tactcaaata aatttattgt tcaaaccaag aatattgata atatcgaggg
    73261 atccaatcca tattttgtat tgaacgatga attgcataaa caggagaaaa tggaacagta
    73321 tgacaatttt aagtctgctc agatatcttt accacagcca ttgatgttta atatctcaac
    73381 tgctgggaaa ggttcgtcgt ctgttggaat gcgtgtatat cgcgaagcaa aagaagtgtt
    73441 gaaacgtgat gataatgatt caaactttgt tctaatctat gagccaaata aaaattatga
    73501 ttggacggat agaaaagtct gggaaatgtg caatcctaac tggggaatat cagtcgattt
    73561 atctgcttta gaatcggcat ttaaaacagc gcaacggtca gctcattcca aagctgaatt
    73621 tctaacgaaa cacttggatg tatttgtgaa cggcgcggat aatttctttg aacaggatca
    73681 agtagagcca tgtttagtta ccacacaaga acttggtgat ttaagtggcg aaccatgtta
    73741 tatcggttta gatttatcac gtacacgaga tttgacctgt gtatctttaa acttcccaac
    73801 atgggatgaa gatggtaaag cggttcttaa agtaaagcaa ctttatttta ttccgaatga
    73861 agatttagag tttcgagaaa aagaagataa tgttccttac agtgatttgg ctgaacaagg
    73921 tttcgttgaa ttttgcgacg gaaaaatgat tgatcaagat cagattttgc agtacataga
    73981 ggactgcatg aatttatatg atattcagca agtgaattat gatccagcga tgagtgacaa
    74041 acttgttgag aaattagaaa acttaggatt ggaatgcgtt gaagttgctc aatatcctaa
    74101 agtattgaat gctccttttg acgatgtcga acggttgttt tatgagaaac gaattcaatt
    74161 tgataatcca ttattccttt attgcacttt gaacgttgta gcaattacta acatcaatgg
    74221 acaaaaagcg ccaagtaaac gtcaatcaaa gaaaaagatt gatgggttcg tggcgttttt
    74281 gtgtggtcat aaggaaacga tgaatcaaat gacagatatt gattcagatg agttagatga
    74341 ttacctaagt tccatctata gataaataga aaggcggtga aaaaaattga aattacgtga
    74401 tcggttatcg aatgcagtct attcttttat ggaaaaacgt ggatatatcg aggatatttt
    74461 tggacactac acacgttatg gtcaaagata tgtgacggat tcatctatca tggaatcttc
    74521 tgatgtttat gaattagttc aagacatatc taatcaggtg gcattggcca caccaattgt
    74581 tattggtccc gacggcaacg aagtcaaaga tcatcactta ctaaatattt tgaagagtcc
    74641 caatgattat ttgacaggat tcgaatttac caaattggaa acaaatactt tattgatcaa
    74701 tggtgagaca ttcccattaa cggataggga tcagcttcat ttggcgtacg gtgtaacaac
    74761 taaaatcaat gaacgacttc aagaagaatt tgaaatgaat ggtcaaaaaa tacctggtca
    74821 aatgattcga cacattaaga acatcggaac tgattcatta aaaggtgctg gaataattga
    74881 tcttgcaaga aacactctag aaggcgttct gagcgctgaa aaagttttga cggacaaata
    74941 tactaaaggc ggtttacttg cgttcatgct taaactagac gcccacatca atccaaataa
    75001 tagcgcccaa acaaaaattg tcaaagctat attggatcaa ctggaaggaa cgcaaaatga
    75061 gagtgatcat tctgttaaga tgattccttt gggaaaagga tattccatcg agacattaaa
    75121 aagtcctgtt gatgacgcgg caattttgaa ttatttaggt gtttacaaaa aagacttagg
    75181 aaaatttcta ggaatcaatg ttgatacgta tcaatcgctg atgaagacag atattgaaaa
    75241 agcgatgatg tatctgcaca acaaagcaat caaaccaata ttgaaaaaca agagcgaaca
    75301 ttacaccgct ctttttttta tgcctaattc tggctataga gtggaatgga aaattaatat
    75361 tttggatttt gttccttact caacaaaaac aaatattggc tacaacatcg ttcgtaccgg
    75421 gattacaagt ccagataacg tggcagaaat gcttggtttt cctaaacaga atactccaga
    75481 aacacaagct atctatattt caaatgactt atctaggatt ggccagaaaa atgcaacaga
    75541 tgattcctta ccaacgaatg atcaagactt gaaaggaggt gatggaaatg aagaagaagg
    75601 aaattcgcac gattgacatc accaatcttt caacacgttc cgatgaagaa actcatacga
    75661 ggaccattag cggctacgct gctgtattca acagtcaaac acttttatgg gatgacttaa
    75721 gcgaagtgat tgttcctggt gcattctcaa aggcaattag taattcggat gttcgttgtt
    75781 tgttcaatca cgattggtcc aatgtgctag gacgtactaa aagcggaacc cttcgccttg
    75841 aagaagatga acgtggtttg aaattcgaag ttgatttacc ggacacaacg gtagcaaggg
    75901 acttagttaa atctatggaa cgaggagaca tcaaccaatg tagttttggt tttgtgccga
    75961 ctgaagaaac gtgggactac aattctgaac caatgcttcg aacaatccat gaagtggaat
    76021 tatacgaggt ttctattgtg cctttgccag catacgaaga tacagaagct gcattaagaa
    76081 gtcgtgatga attagaaaaa aacgtcgaag aaagaaaaaa attaatcaaa aaaattaatc
    76141 aagcgctaga agcgtaggag gaaaaataca tggataaaga attattgaaa aaaatgaagg
    76201 cacgtcgtga gcaacgattg actgaattac gtgaaaaagt tgaatcagga gaattacgcg
    76261 aagcagattt agaagccgtg aaagaagaaa ttgacagtgt tattgatgaa ttgaacggaa
    76321 ttaaagacga attaggcgca gattctggaa ctgatgaaac agacgacaat acagatgatc
    76381 aatcgaatag tacggattca gacgaaagcc gttctggtga agacaacgat caagaagaag
    76441 attcagacag tgaagatagt tcagaaaatc gttctggaat gattactcaa cagcaacgag
    76501 atggattact tggatcaatt aagaacggat tggaggcacg tgcaaaaatg accaagaaac
    76561 aaaaagatca acaactacga aaagcatttg ctaattttgt agctggaaat atttctgaag
    76621 cagaagctcg agctttaggg attgaagctg gcaacggttc agttactgtc ccagaagtaa
    76681 ttgcatctga agttattact tatgctcaag aagaaaattt acttcgtaaa tacggaacag
    76741 tggtgcgaac atcaggagat gtcaaatatc caattcttgt gaagaaagca gatgcgaatg
    76801 taaacaagaa agagcgttca actgatattg ctgaaacagc tattcagttt gatgaaattt
    76861 tgcttaatcc tgccgaattc gatgctttgg caacagtaac taaaaaatta ttaaaaatgt
    76921 ctggtgttcc agttgaagat attgttgtgg aagaattgaa aaaagcttat gtgcgtaaag
    76981 aaatcaatta tatgttcaat ggtgatgacg ctggaaatga aaatcctggt gcattagcca
    77041 aaaaggctgt agcatttaaa aaacctttag atctaactgc ttcaggtgct gggcaaaaat
    77101 tatatgatgc attaatcgaa tttaaaaata caccagtgac agaagtgatg aaaaagggac
    77161 gctttattat taatcgagct gctttgactg ccattgaaaa aatgaaaaca gatgatggat
    77221 ttcctttgtt gcggccattc acacaagcag aaggtggaat tggttaccaa ttagttggct
    77281 atcctgtgga ttggacagat gcagcagata aaaagggtga accagacacg ccagttttat
    77341 attttggcga tttttctgca tttaaaattc aagaagttat tggtgccttg gaaattcaaa
    77401 aacttgttga aaaattctct ggtaaaaatc aaattggatt ccaaatttac aacttgctag
    77461 atggtcaatt ggtttattct ccatttgaac cggcagtata tcgctacgaa attacaaaac
    77521 cagttggtgg ttaagatgga agagcaaact aaagaattgt ctttagagga aaaattcaaa
    77581 tcacatattc attttgaaga gggcatggat gattctttgc tctcttttta tttaaatatg
    77641 gcaaaagatt atgtcaaaac agcaactggt ggccaacaag aatatcttat tttgatggtt
    77701 gccggcattg cctatgaata tagagtttca gaggatgaac tcgacaaagc tatgaatgcc
    77761 atgacgccat ttatcgtgca aggagcgatt caaaatgccg aagagacaga ccaataatct
    77821 cagatggaaa gccgaattgc tagacatcaa aacaggaaca gacggaaacg atcgtccaac
    77881 tacaatttac gaatttaagc gtctaatatt ctatgaagaa ctcggtgtga cttctcaaga
    77941 aaaatattta tcacagcaag ccaagacaga cgttgtcaga cgaattaaag tcagatggga
    78001 taaatccatc acagagaaat taagtgcgct caaaattgat tctgtaacgt ataacattac
    78061 tcgcatttat acgaatcccg atgcaagaga aatggagttg agtttagctt atgtcgatta
    78121 gctttgatga attgaaaaca gcgctgaaat caacaaagtt agcagtgttc agagacaaag
    78181 ccagattagg gacgatgtat ccatacattg tgtactcaaa tgtgagtaac agcaaaaaaa
    78241 tggcatccgg taaagtctat aaaaaattac cttattacca agtctctttt tttacacttg
    78301 gaacagaaca ggatttagtt gttattgaac aagcgctgca aaatgctggc attccatatt
    78361 cagattttac aggtatacaa ggtgatgaga acgacgatac cgtaaccaac tattacacat
    78421 atgtgaggtg tgtggaaaat gccaagtaat aagaatggtt tttctgaaat atcggattat
    78481 ttaggaaacc tttcaaaagt tgatccgaaa aaattatcgt tggaatcttt agaagaagca
    78541 gctaactttt atctaaagca gttgcttcct aatattccta agtcgctctt aaaaaagaaa
    78601 catatgcgcg atcaaataaa agtagtagtt gaaaacgatc aagtgaaagt tcaatttgaa
    78661 gagacggcct tttactggcg ctttgccgaa aatggcacaa caaatcaacg tgcccaacat
    78721 tttgcaagtg gtacgtatga acaaaacaaa gaaaaaattg aagaaattat gactagaaaa
    78781 atacttgatt tatgggaagg gtgattttaa ttgggaaaac aagatacttt ttattttgaa
    78841 ggattggacg acattttaat tgcgatgatg gcaacgccag attctgttgg aactgcaccg
    78901 acttatagtg aagttttacg attgccaatt gccacaaaaa tcggtgtcaa agggaatgga
    78961 acagcattag aaaaatgggc atccagcaaa atgtttcgtc gtgtgtcgcg agaaacaaaa
    79021 catgaacttg cgttggatca cgtgggtatt ccgatagaag tgatggatga aatcaaagga
    79081 ttagttgcta aaagcggagt aacgtttagc aaaaataccg cacgagaatt tccgtacttc
    79141 gcctttggat ttattggaaa tattgaaaat ggcggtaaaa aagcagtttg gtatcctaat
    79201 acacaactat ccaatgtgat tgacgaagag tatgccactg cagaggaaga aacaaaaatt
    79261 gacgatgtaa ctgcaaatct ggtttccatc ggactgaaat acaataatgt gatgtatgca
    79321 agttttgatt caaatagaga cggagcttct ctagaattat ttaataaatt cattgctcaa
    79381 cctgtatacg atgaagaaca gtggaaaaca ttggctaaag ttggaggtgc gggttaatgg
    79441 ctcggttatc tgattatgga attcacgttg aagacttaaa aaattctgct gttgtaatta
    79501 ttcaaggcgc agaattccct atttcattta ctatgcaaac gatggaattt atagcagatg
    79561 tgtatggtgg agattattcg caatttgaat ctgatatgaa cgccatgcta tctaaaaaag
    79621 aaggacaaat ttcttctgct aacttatcgc ctagtgatct aaaaatcatg cgtgccttga
    79681 tttatgccat gctgcgtact ggtggtttag aggaagatcc agaaactatt ttcaaatttt
    79741 tgggaatgag tggagaggtg ttgtctgctt atagtacctg tatggaaatt tttgctagcc
    79801 agacatttca ggtggaagac ctaaaaaaat ccaagaagcc acaagacttt caaaaagcgc
    79861 aagcaaaaag aaagaaaaac aaaaagaatc ggaagagata ggaactcctt ggagttttta
    79921 tatttacgtt gctctcactc tattgaattg gagtgagagt ttctttttaa agtctacacc
    79981 taacttgtgg ctcaaatctt atttacagtg gttacaacaa aacaccgatt ttgaaccacc
    80041 tcaatctgta actatggata aatcgccttg gtggtagaaa ggagcgctaa gataaatggc
    80101 tggtaaagaa tctgatgtcg ttcttaattt taaaacgaat ggcgaagtca gttattcgaa
    80161 aacaatcaaa gaaatcaaca aagaaatgaa cttagccgct gccgagtaca aaaaccaagt
    80221 gtctgcgatg gacaaggatg caactcaaac agaaaaattg cgagcggcta aacaaaaatt
    80281 agaaaaacaa ttaggattgg ctgaacaacg atctcaaatg ctgagagagg aatatgaaaa
    80341 atctgtcaaa gaaaccggtg aatattctgc tgaatcagaa aaactttata aacaattact
    80401 caattctgaa accggagaaa ataaacttcg tacagcatta gaacagacga acgatgcact
    80461 taaagaacaa ggtgacgttt ctgttgatac agcaaaaaaa ctccagaaaa tcgaagaaac
    80521 aggtgagaaa gtaaaaggcg ttggtgaaaa aatgtctgtt ggagtaaccg cgcctattgt
    80581 agcggcagga gcagcaggac ttgcagcatt tggtgaagtt gacgaggcac ttgataccat
    80641 cattacaaaa accggagcaa caggtgatca agctgataga ctttcacagt ctttcaaaaa
    80701 cgttggttca aatactcatt tacctttaca aacggttggg gaagctattg gtgaggtaaa
    80761 tacacaattt ggattcatgg ataaaaaact ggaagattca accaattatc tcctacagta
    80821 cgctgaaatc aatgatacag atgtttcgca atcagcaata tctgctcgac aagctattga
    80881 tgcttatgga ctagaatatg atgatttgaa ttctgtcctt gatgtaacaa cgaaaacatc
    80941 gcagaatact ggtcaatctg tagacgactt gatgcaaaaa gcaattgatg gcgcaccaca
    81001 aattaaacaa ttagggttga gctttggtga aggggtcact ttacttggac aatttgagca
    81061 atctggtgtt gattcaagcg cagctttaag tagtttatct aaagcaacag tggcttatgc
    81121 gaaagacgga aaatctttaa gtcaaggact agaagaacta caagacaagg taaaaaatgc
    81181 aggttctgaa acagaagcca tcaatgcagc agcagaagta ttcgggacta aaggcggtcc
    81241 tagaatggcc gatgcaattc gtagaggtac tttgaacctg gaagatttag caaaaactgc
    81301 tggagaaagt gggggagctg taggagacac attcgatgct actctcgacc caattgatca
    81361 agccgatcaa gctatgaaca atgcaaaatt agcaatggct gacgtaggtg aagcagtcca
    81421 agtaagcctt ttgccttttt ttgaaaaagc aacttcctta ttacaggaat tctctaaatg
    81481 gtgggggtct ctagatcaag atacaaagaa ttggatcata acaattgctg gtatagcggc
    81541 agcagttgga ccagtactaa tcgttcttgg ttcacttatg ggatctgtta caaaaatcgt
    81601 tggggaagtt aaaagtttta taggagtttg gcaaggactt gctggtttat ttggaatgtc
    81661 gggaggctgg tttgcattag cagttatagc tattggcgca ttagttactg gactaatttg
    81721 ggcatataat aacgttaaat ggtttcatga tggagtgaat tctttcttca aaggtgtttc
    81781 agatgtagct gttgaaatat ttaatttttt aagtggttat tttggtggat ttttcgatgg
    81841 tgttatagca aatttcaata atttttataa tgctggaaaa cgaattttca gtggttttct
    81901 agattttatc actggagtat ttacagggaa ttggtcaaaa gcatggcaag gtctagttga
    81961 tatttttgga ggtattttcg atggtattgt tgctgtaggg aaagctccta ttaatgccat
    82021 gattggattg atcaatggat ttattggtgg attgaataat ataaagatac caaaatgggt
    82081 tcctggtgta ggtggaaaat cattttctat ttcaaaactg ccttatttag cacaaggcgg
    82141 tcatctaatc aatggacaag cgattgttgg tgaagctgga cctgagttac tgactgccaa
    82201 gaatggtaaa accacagtta ctccattatc agacgaagaa aaacgcaaag ggatcagtgg
    82261 aagagtttct ggaggtagca ttgaacagca tatacacatt ggcaaagtcg atgcaaacaa
    82321 tccatctgag ttagatcgta tgaatcgtaa atttgctaaa gcaaacaaac aggctattcg
    82381 tgatttggga ggtgttccta tttgagtcga caatttatga atccagatga accaaatttt
    82441 atttggaaaa atcggaatgc agtaattgat atgaattgca tcattgaggc agagcttcca
    82501 gatattatgc cgaacaagcg atacgaaact tacacgattc agggccgaag cggagaattg
    82561 acggagacgt ttgatgacta tgaacctttt gatttggaaa tagaagggat cactattcca
    82621 cattcgaaac tgagagaagt caaacgatgg ctcactggta aaagccaatt aatcacacat
    82681 aatgatccag acaagtatct agaggctatc tgcagcatgg ataaagaagt ccctttcgaa
    82741 aacgagtggg gcttctttta tacattcgat gttacttttc gttgtcagcc actcaaaaga
    82801 aaaatcggtg aagcacctaa aatttttcat tctagtgtta tggatatttt tgatcatggt
    82861 gacgaaattg cacatcccta tctggaaata gaatcgaacg gaggagatat aacgatcgct
    82921 gtcggtgata aaagcctaac aatacttaat acgttagctg caacaatcgt ggttgatact
    82981 cagcttggaa aagcaataca agaaggtttg aatttattta cgcgtggtga ttggccagta
    83041 ttgcagcctg aatggaatca ggtaaaaata tccggaaaat ttaaagaagt gcggttttgg
    83101 aataggagtg tgtatctgtg acgcaagaat ttatttatgc ttataaaaaa atgccagatg
    83161 atttgagcgt gaacggagcg tcgttagttg attgggaaga tttgccagaa atcaatcgtg
    83221 tgctgaatgg ccaatatcgt ttttatggta actattcaag aagcggacag tatcgctctt
    83281 atttaaaaaa aggaaatttt ataaaagcga aggtaccaga tggatcatgg caatattttg
    83341 aaatttataa tgtcaaaaag aatttgacct ccgtttctgt aacagctcgg catattggtt
    83401 ttatggccaa taagaacttt attgtcaagt cgtttacgga taacggcaat gggtctcaga
    83461 tcatgaccaa tcttaaaaac agtttggcat ttgatcaaaa atttaactat ctttcgaatg
    83521 tagggacaac gcaccaattt acagcaagac aagtcgcgcc agttgaagca atcattggtt
    83581 caaataacgg taatcaaaat ttgactggcg tgactagcgc ggaacttgat atggataact
    83641 atgatttgaa actggtaaaa cagattggat cagataatgg ttttcggatt gattttggca
    83701 tcaatttaga agcaatcgaa gaagaaattg atgaagaatc aattgtcaat agtttgtatt
    83761 tggttggcgg tgttcctgat aacgattatg atgaagacaa agaacctatc gaatatggtt
    83821 atttagaaat tgatggagta accaacgaga atcgacgtat tgccaaacgt gaaaacggag
    83881 attgtaaaac agttgatgaa ttgatcaaat ggggcaaaac cttatttgac aatgatcgta
    83941 tccatgagcc aaaagctact catacagtca gtatggtggc gttggaacac acactcgaat
    84001 acggtgacat gtatcaagag ctagcgtctt tacactttgg tgatgtcgct catgttaggg
    84061 cgaaagagct agatatcgag ataaaagagc gtatggtcga atacacttat ttcccaacgc
    84121 ttgggaaata taaggatatt gttttaggta atgatttatc actgtacact tcggcagtaa
    84181 atactcaagc acaagaattg aaaaagaaaa tcgacaatcg aacagaaacg ttagttcaaa
    84241 atgtgttgaa tgcgacggca tggatcactg gtaattctgg tgggcatgtc gtttttcgtc
    84301 cagaaaaagc tccatcagag attcttatca tggatactcc aaatgtggcg aatgcaaagc
    84361 gagtgtggcg ttggaattta aatggcttag gttattcaga caacggagta aatggcccgt
    84421 tcggtattgc tatcacatca aaaggagaaa ttgttgctga cttcatcaaa gtaggaacaa
    84481 taaatgcaga agtattcgaa acttccttta atgcttatgg tgatgtgtta aaacttgtaa
    84541 aaggcacgct tcaaatttgg aatgaaaaca aaaaaatcat ggagctaacc aaaaaaggta
    84601 tggaattctg gaatagaaaa gaatccattg gaacgatcgg aacaacagat tcagcaggaa
    84661 atccatttcc aaatgctgta acacctacac ctcttgagga aaattctttg gtaatacgta
    84721 caaatggcga tggaaaatac attttaattt ctccaactgc agaaaaagga tttgtgttat
    84781 taggaaacgg aaaagcatat tatttcggtg atttagatat tcagggaaaa cttactgtta
    84841 gaggtaaaga agtgatacca ggacaaaatg gcggtccatc tggcggagga gaaacacctg
    84901 gtgggtatcc tgatgaatta aaaacagatg cagaaaagag agcttggaga at
//
LOCUS       DAZKOK010000002        61970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000002 DAZKOK010000000
VERSION     DAZKOK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.002"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1016
                     /locus_tag="ACG90W_000111"
     CDS             153..1016
                     /locus_tag="ACG90W_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0476508.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1145..2767)
                     /locus_tag="ACG90W_000112"
     CDS             complement(1145..2767)
                     /locus_tag="ACG90W_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0476509.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3016..3537
                     /locus_tag="ACG90W_000113"
     CDS             3016..3537
                     /locus_tag="ACG90W_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0476510.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3723..5453)
                     /gene="spxB"
                     /locus_tag="ACG90W_000114"
     CDS             complement(3723..5453)
                     /gene="spxB"
                     /locus_tag="ACG90W_000114"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0476511.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5618..6436
                     /locus_tag="ACG90W_000115"
     CDS             5618..6436
                     /locus_tag="ACG90W_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0476512.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6539..6736)
                     /locus_tag="ACG90W_000116"
     CDS             complement(6539..6736)
                     /locus_tag="ACG90W_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476513.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6905..7483
                     /locus_tag="ACG90W_000117"
     CDS             6905..7483
                     /locus_tag="ACG90W_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0476514.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7578..7715
                     /locus_tag="ACG90W_000118"
     CDS             7578..7715
                     /locus_tag="ACG90W_000118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476515.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8528..9031)
                     /locus_tag="ACG90W_000119"
     CDS             complement(8528..9031)
                     /locus_tag="ACG90W_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0476516.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9390..10415
                     /locus_tag="ACG90W_000120"
     CDS             9390..10415
                     /locus_tag="ACG90W_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0476517.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10738..11616
                     /locus_tag="ACG90W_000121"
     CDS             10738..11616
                     /locus_tag="ACG90W_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0476518.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12092..12208
                     /locus_tag="ACG90W_000122"
     CDS             12092..12208
                     /locus_tag="ACG90W_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0476519.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13136..13474
                     /locus_tag="ACG90W_000123"
     CDS             13136..13474
                     /locus_tag="ACG90W_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476520.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13568..13798
                     /locus_tag="ACG90W_000124"
     CDS             13568..13798
                     /locus_tag="ACG90W_000124"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0476521.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14198..14569
                     /locus_tag="ACG90W_000125"
     CDS             14198..14569
                     /locus_tag="ACG90W_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0476522.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14843..15535)
                     /locus_tag="ACG90W_000126"
     CDS             complement(14843..15535)
                     /locus_tag="ACG90W_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0476523.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15538..16323)
                     /locus_tag="ACG90W_000127"
     CDS             complement(15538..16323)
                     /locus_tag="ACG90W_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0476524.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16560..17960
                     /gene="lacG"
                     /locus_tag="ACG90W_000128"
     CDS             16560..17960
                     /gene="lacG"
                     /locus_tag="ACG90W_000128"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0476525.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17985..18302
                     /locus_tag="ACG90W_000129"
     CDS             17985..18302
                     /locus_tag="ACG90W_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0476526.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18315..19988
                     /locus_tag="ACG90W_000130"
     CDS             18315..19988
                     /locus_tag="ACG90W_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0476527.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20002..21633
                     /locus_tag="ACG90W_000131"
     CDS             20002..21633
                     /locus_tag="ACG90W_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0476528.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22124..23689)
                     /gene="guaA"
                     /locus_tag="ACG90W_000132"
     CDS             complement(22124..23689)
                     /gene="guaA"
                     /locus_tag="ACG90W_000132"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0476529.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23748..24671)
                     /gene="coaA"
                     /locus_tag="ACG90W_000133"
     CDS             complement(23748..24671)
                     /gene="coaA"
                     /locus_tag="ACG90W_000133"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0476530.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24867..25472
                     /locus_tag="ACG90W_000134"
     CDS             24867..25472
                     /locus_tag="ACG90W_000134"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0476531.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25780..26796
                     /locus_tag="ACG90W_000135"
     CDS             25780..26796
                     /locus_tag="ACG90W_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0476532.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26937..28238
                     /locus_tag="ACG90W_000136"
     CDS             26937..28238
                     /locus_tag="ACG90W_000136"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0476533.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28284..28946
                     /gene="deoC"
                     /locus_tag="ACG90W_000137"
     CDS             28284..28946
                     /gene="deoC"
                     /locus_tag="ACG90W_000137"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0476534.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28967..29365
                     /locus_tag="ACG90W_000138"
     CDS             28967..29365
                     /locus_tag="ACG90W_000138"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0476535.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29431..30513
                     /locus_tag="ACG90W_000139"
     CDS             29431..30513
                     /locus_tag="ACG90W_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0476536.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30725..32287
                     /locus_tag="ACG90W_000140"
     CDS             30725..32287
                     /locus_tag="ACG90W_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0476537.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32280..33413
                     /locus_tag="ACG90W_000141"
     CDS             32280..33413
                     /locus_tag="ACG90W_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476538.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33413..34369
                     /locus_tag="ACG90W_000142"
     CDS             33413..34369
                     /locus_tag="ACG90W_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476539.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34468..35634
                     /gene="deoB"
                     /locus_tag="ACG90W_000143"
     CDS             34468..35634
                     /gene="deoB"
                     /locus_tag="ACG90W_000143"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0476540.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35658..36476
                     /locus_tag="ACG90W_000144"
     CDS             35658..36476
                     /locus_tag="ACG90W_000144"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0476541.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36493..37200
                     /gene="deoD"
                     /locus_tag="ACG90W_000145"
     CDS             36493..37200
                     /gene="deoD"
                     /locus_tag="ACG90W_000145"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0476542.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37498..38184)
                     /gene="gpmA"
                     /locus_tag="ACG90W_000146"
     CDS             complement(37498..38184)
                     /gene="gpmA"
                     /locus_tag="ACG90W_000146"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0476543.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38356..38937)
                     /locus_tag="ACG90W_000147"
     CDS             complement(38356..38937)
                     /locus_tag="ACG90W_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0476544.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39070..39753)
                     /gene="rpiA"
                     /locus_tag="ACG90W_000148"
     CDS             complement(39070..39753)
                     /gene="rpiA"
                     /locus_tag="ACG90W_000148"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0476545.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40072..40485
                     /gene="rpsL"
                     /locus_tag="ACG90W_000149"
     CDS             40072..40485
                     /gene="rpsL"
                     /locus_tag="ACG90W_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0476546.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40569..41039
                     /gene="rpsG"
                     /locus_tag="ACG90W_000150"
     CDS             40569..41039
                     /gene="rpsG"
                     /locus_tag="ACG90W_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0476547.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41133..43220
                     /gene="fusA"
                     /locus_tag="ACG90W_000151"
     CDS             41133..43220
                     /gene="fusA"
                     /locus_tag="ACG90W_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0476548.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43392..44579
                     /gene="tuf"
                     /locus_tag="ACG90W_000152"
     CDS             43392..44579
                     /gene="tuf"
                     /locus_tag="ACG90W_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0476549.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            44986..45294
                     /gene="rpsJ"
                     /locus_tag="ACG90W_000153"
     CDS             44986..45294
                     /gene="rpsJ"
                     /locus_tag="ACG90W_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0476550.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45325..45954
                     /gene="rplC"
                     /locus_tag="ACG90W_000154"
     CDS             45325..45954
                     /gene="rplC"
                     /locus_tag="ACG90W_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0476551.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            45982..46605
                     /gene="rplD"
                     /locus_tag="ACG90W_000155"
     CDS             45982..46605
                     /gene="rplD"
                     /locus_tag="ACG90W_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0476552.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46605..46895
                     /gene="rplW"
                     /locus_tag="ACG90W_000156"
     CDS             46605..46895
                     /gene="rplW"
                     /locus_tag="ACG90W_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0476553.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            46934..47767
                     /gene="rplB"
                     /locus_tag="ACG90W_000157"
     CDS             46934..47767
                     /gene="rplB"
                     /locus_tag="ACG90W_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0476554.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47810..48088
                     /gene="rpsS"
                     /locus_tag="ACG90W_000158"
     CDS             47810..48088
                     /gene="rpsS"
                     /locus_tag="ACG90W_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0476555.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48111..48458
                     /gene="rplV"
                     /locus_tag="ACG90W_000159"
     CDS             48111..48458
                     /gene="rplV"
                     /locus_tag="ACG90W_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0476556.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48472..49128
                     /gene="rpsC"
                     /locus_tag="ACG90W_000160"
     CDS             48472..49128
                     /gene="rpsC"
                     /locus_tag="ACG90W_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0476557.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49131..49565
                     /gene="rplP"
                     /locus_tag="ACG90W_000161"
     CDS             49131..49565
                     /gene="rplP"
                     /locus_tag="ACG90W_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0476558.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49555..49743
                     /gene="rpmC"
                     /locus_tag="ACG90W_000162"
     CDS             49555..49743
                     /gene="rpmC"
                     /locus_tag="ACG90W_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0476559.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49768..50034
                     /gene="rpsQ"
                     /locus_tag="ACG90W_000163"
     CDS             49768..50034
                     /gene="rpsQ"
                     /locus_tag="ACG90W_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0476560.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50092..50460
                     /gene="rplN"
                     /locus_tag="ACG90W_000164"
     CDS             50092..50460
                     /gene="rplN"
                     /locus_tag="ACG90W_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0476561.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50497..50805
                     /gene="rplX"
                     /locus_tag="ACG90W_000165"
     CDS             50497..50805
                     /gene="rplX"
                     /locus_tag="ACG90W_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0476562.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50833..51372
                     /gene="rplE"
                     /locus_tag="ACG90W_000166"
     CDS             50833..51372
                     /gene="rplE"
                     /locus_tag="ACG90W_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0476563.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51391..51576
                     /locus_tag="ACG90W_000167"
     CDS             51391..51576
                     /locus_tag="ACG90W_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0476564.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51612..52010
                     /gene="rpsH"
                     /locus_tag="ACG90W_000168"
     CDS             51612..52010
                     /gene="rpsH"
                     /locus_tag="ACG90W_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0476565.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52042..52578
                     /gene="rplF"
                     /locus_tag="ACG90W_000169"
     CDS             52042..52578
                     /gene="rplF"
                     /locus_tag="ACG90W_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0476566.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52739..53095
                     /gene="rplR"
                     /locus_tag="ACG90W_000170"
     CDS             52739..53095
                     /gene="rplR"
                     /locus_tag="ACG90W_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0476567.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53116..53616
                     /gene="rpsE"
                     /locus_tag="ACG90W_000171"
     CDS             53116..53616
                     /gene="rpsE"
                     /locus_tag="ACG90W_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0476568.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53631..53810
                     /gene="rpmD"
                     /locus_tag="ACG90W_000172"
     CDS             53631..53810
                     /gene="rpmD"
                     /locus_tag="ACG90W_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0476569.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53854..54294
                     /gene="rplO"
                     /locus_tag="ACG90W_000173"
     CDS             53854..54294
                     /gene="rplO"
                     /locus_tag="ACG90W_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0476570.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54294..55589
                     /gene="secY"
                     /locus_tag="ACG90W_000174"
     CDS             54294..55589
                     /gene="secY"
                     /locus_tag="ACG90W_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0476571.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55651..56298
                     /locus_tag="ACG90W_000175"
     CDS             55651..56298
                     /locus_tag="ACG90W_000175"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0476572.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56489..56707
                     /gene="infA"
                     /locus_tag="ACG90W_000176"
     CDS             56489..56707
                     /gene="infA"
                     /locus_tag="ACG90W_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0476573.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56740..56856
                     /gene="rpmJ"
                     /locus_tag="ACG90W_000177"
     CDS             56740..56856
                     /gene="rpmJ"
                     /locus_tag="ACG90W_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0476574.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56874..57239
                     /gene="rpsM"
                     /locus_tag="ACG90W_000178"
     CDS             56874..57239
                     /gene="rpsM"
                     /locus_tag="ACG90W_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0476575.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57267..57656
                     /gene="rpsK"
                     /locus_tag="ACG90W_000179"
     CDS             57267..57656
                     /gene="rpsK"
                     /locus_tag="ACG90W_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0476576.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57736..58674
                     /locus_tag="ACG90W_000180"
     CDS             57736..58674
                     /locus_tag="ACG90W_000180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0476577.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58702..59082
                     /gene="rplQ"
                     /locus_tag="ACG90W_000181"
     CDS             58702..59082
                     /gene="rplQ"
                     /locus_tag="ACG90W_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0476578.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59354..60193
                     /locus_tag="ACG90W_000182"
     CDS             59354..60193
                     /locus_tag="ACG90W_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0476579.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60169..61038
                     /locus_tag="ACG90W_000183"
     CDS             60169..61038
                     /locus_tag="ACG90W_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0476580.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61028..61825
                     /locus_tag="ACG90W_000184"
     CDS             61028..61825
                     /locus_tag="ACG90W_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0476581.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        19617 a        11084 c        13191 g        18078 t
ORIGIN      
        1 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcttatttt gattagaact
       61 tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga tttgctataa tagacgataa
      121 gggtaaaatg taacgagaag gggttgaaaa atgtgtatca aattgtaaca gattcttgtt
      181 gtgatctgcc ataccatgta ttagcaaaag aaaaagttga ttttttatca atgaatctcc
      241 aattgaatga tcaagagtta gtagatgacc tcggaaagac attcaattat gaccgctaca
      301 tgaatcagct gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt ggccgatatt
      361 tagaattttt ccgttcttat gcggaaaaac agattcctgt tttatatgtg gctttttcca
      421 gtggattaag cggctcttac agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat
      481 atgaagaccc tgagatctat gtttttgata caaaagctgc aagtattggg gaagggcttt
      541 tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa accctagagt
      601 ggctggaaag taattatttg aaagtccatt catgggtaac agtagacgat ctgaaatatc
      661 tagaacgagg cggtaggatt tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa
      721 agcctattat tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa
      781 gaaacaagtc gatccagaca atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg
      841 atcaaatcgt ttatgtggct tacgctgggg atctagagag cgcggagaag gcaaaacggc
      901 ttttggaaga gcagataaag atgaaagatg tgaagcttta tcccttagga ccaacgatcg
      961 ccagtcacac aggctatggc tgtatcgcga ttttttcgat gggaatcagt cgataaaagc
     1021 agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc gactcttaaa
     1081 agatagatga gcatcctatc tcttagaggt cgctgttttt ttatttttta agctaaatag
     1141 gccattatgc ccataactcg ttcaattgtt tttgaatatc tgcattttcc aaaaattcat
     1201 catatgtcgt atcagctcga tcgatcacac ctttatccga aacggcaatg atgcggtttg
     1261 ccaatgtttg gataaattga tggtcatgtg acgcaaataa gattgagcca gtaaatgcca
     1321 tcaagccgtc attcaatgct gtgattgatt ctaagtctaa atggttcgtt ggatcatcta
     1381 atacaagtac gttagctttc gaaagcatca tttttgaaag catgcaacgg actttctctc
     1441 caccagataa aacattcact ggtttcaata cttcttcacc agaaaacaac attcttccta
     1501 agaagcttct taagaaggtg ttatcatctt cttctttact tgcatattga cgcaaccagt
     1561 ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc ctttggtaaa taagaattac
     1621 ttgttgtcac accccaacgt actgttccag tatctggcgt aatttcaccc atgataactt
     1681 tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg tcttctttgc
     1741 tcaaattaaa agatatatca tctaaaattt tctttccatc aatcgttaca cttacatttt
     1801 ctacctgcaa taagtcgttt ccaatctcac gttcaggttt gaatccaacg aatggataac
     1861 gtctagaaga aggttggata tcgtctaaag taattttttc aagcattttt ttacgtgaag
     1921 ttgcttgttt tgattttgat gcattcgcac taaagcgggc aataaattct tggagttctt
     1981 tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag tttagccgct agttgactag
     2041 attccaacca aaaatcataa tttcctacat aaagtttgat tttaccaaaa tctagatccg
     2101 ccatatgtgt acatactttg ttcaaaaagt gacggtcatg ggatacaacg atcacagtat
     2161 tttcaaaatt gatcaaaaat tcttccagcc aattgatcga ttggatatcc agaccgtttg
     2221 ttggttcgtc tagcaaaaga acatcaggtt taccaaatag tgtttgtgct aataacactt
     2281 ttaccttttg tccaccagtt aattcactca tttgctgatg gtgcaattct tcagggatgt
     2341 tcagaccttg cagtaaaaca gctgcttctg gttcagcttc ccatccatta agctcagcaa
     2401 attctccttc taattcagct gctcgaatac catcttcgtc agtgaaatcc tctttcatgt
     2461 aaatcgcatc tttttctttc agcacttcat acagacgctt gtgtcccatg atgactgttt
     2521 caagtacagt atagtcttca tagtcgtaat ggttctgttt caatacggcc atccgttcat
     2581 ctggtcccat agtaacggaa cctgtggaag gctgcagatc ccctgataac actttaagga
     2641 acgttgattt acctgcacca tttgcaccaa tcaagccata gcagtttccg ggggtaaatt
     2701 taatattcac atcatcaaat agtttgcggt ctgaaaagtg caaactcaca tcatttacag
     2761 taatcaattt ttttcctcac ttttatcatt attagttttg acaagtctta tagaattata
     2821 taggggaaag gtaaaagatt caaggcaatc gaacattctt tgtgtttcac gtgaaacaga
     2881 atgaaaaaaa taaaaataaa agcggagcga aaatagctac tataggattt attagtttgc
     2941 aacagcataa tttttgacgt tatttataag agggagtact ataaaactgt aaaagaaatg
     3001 aaagaaggat tgaaaatgga tgaaaaaaga accatcgact ggggctcatt gattttggga
     3061 attttatttg tacttgtctc attgttatca tttcaagacc ctgtcggaaa tttagttgca
     3121 attgtagtag tctttgcgat ttttgccttt attaaaggga tatttgaact gtttgtacgg
     3181 aacagattaa aagagctaac aggatataaa ggaaagatgc ccttaataat cgggatcatc
     3241 gatattctag ttggcgtctt tttcttattc aatattggtg caggagtcgc agcattgcct
     3301 tttgtttttg cagtatggtt tattgctgat tcagttttag cgttatttac tgctgacttg
     3361 gcaagaggag taagtgaagg atattattgg tttacgatca tcgtcaatat tttaggaatt
     3421 ttattaggaa ttttcttatt gtttaatcca atttcttctg cgttgacatt aagcttttta
     3481 gttggattct attttatgtt atttggtatc acacatatcg tctatgcatt tagataagta
     3541 aaatcaaaca aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc
     3601 ggctttttta tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat
     3661 gcaaaaatgt gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt
     3721 tgtcacaacc tcttttcata taattcattc aatactggca aatcatggac ttcatatttt
     3781 tctttgaatg cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct
     3841 actggcaatg ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt
     3901 gcagctgcat caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc
     3961 ccgagcgctt ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt
     4021 tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca
     4081 ttaataattg gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac
     4141 ccgccatcac cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt
     4201 cctcctggca caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta
     4261 ccgttcatat tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa
     4321 atcgcatcgt cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt
     4381 tcttcctctc gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga
     4441 tttgctaggt accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa
     4501 gctgtgtttg catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac
     4561 gcatcgatat cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg
     4621 aagtcgctac cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta
     4681 gtagcgacac gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt
     4741 gctagaacgg aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga
     4801 aatccgtttc gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa
     4861 ggcaaagcag ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga
     4921 gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg
     4981 atagccacgc ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga
     5041 ctttgtggtg tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca
     5101 tttagttcct ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct
     5161 actagaggca catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca
     5221 ggtcctgcag agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct
     5281 gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga
     5341 tatagcgcat ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata
     5401 ccccagcttt caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt
     5461 cactccttta gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc
     5521 gaaaatccca actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta
     5581 gagtagtaca tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact
     5641 tgaaccttgg cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg
     5701 tgccgggttg gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg
     5761 atttgcttct ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac
     5821 aaaagcagaa gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg
     5881 cggtgtgttt ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca
     5941 tgaagcagaa ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac
     6001 attgcataac attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc
     6061 ggataatccg acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca
     6121 aaacttccca gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa
     6181 aaaggccttt gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg
     6241 agcgctacta gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg
     6301 agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag
     6361 caaacgtcat tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga
     6421 tgtggcatta ggataaataa aaaaaatata tatgatatat caaatagggc cgcgaactta
     6481 tatcgcgacc ctattttttt agtaagttat atcataatga agtgaatggt atcatacatt
     6541 aataatagtt ttttctatgt gtatttcctt tgatccagcg aacaagccag atgataagtg
     6601 cgattgggat tagaactttg accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa
     6661 gactgatgac gaagctagct gcaattagca ctaaaagaac catgaagata tttaagaaca
     6721 tatttttttg acgcatacaa aaccctcctc ttgttcattc tttactatag aaaaagtata
     6781 acagggattg tttaaaaaca cctccaactg aagactgatt tttacttcat ctacttaaat
     6841 gatcaaacaa ttgctttttc tctggtttat catctaaaat agagcggaaa aggaggagaa
     6901 tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct gtaatcgcga ttatcatgac
     6961 tattttagtt ttagaacttc atgaacctaa cggagacacg tgggaggcgc tcgctgcatt
     7021 aggttaccga ttttttatct atattgtcag ttttatcatt ttagcgattt attggaacaa
     7081 tcatcatcat ttatttcaag ttgtacataa gataaatgga cgggtcttat gggcgaacag
     7141 tttctttgtt tttgcgcttt cgctttttcc gtttgcaact tcatggatca gtgagtacat
     7201 caatagcctg gctccagaaa tgacttatgg ttttgttacc ttgaccgcta atattgcgta
     7261 ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc
     7321 gatgttgaat aattacaaga aatcgtatct atcgattggt ttgaatgtgt taggaattgt
     7381 cttagggtat ttagtagcgc cagtagccgt gctgatcgca aacatcgtga ttctagctgc
     7441 ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa
     7501 atttaagaga aaaatataga aaaaataaat aaacgatcaa atatccgagt gatactagta
     7561 ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt aggactgatt actagttttg
     7621 tcacgatgct tatttatttg gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag
     7681 gatttttgac attattcaca gcaataaaaa aataaacatc tttatgtaat ctgatgttct
     7741 atctatttaa gaaaagcatg tttacagcag tatgattctg ttgtaaacgt gtttttattt
     7801 gtggaaaatt taatttttag aaaagtgtat cgaaaaatag atagttagtt tttccacagt
     7861 ggataacttc gtaagagata aaaataaaaa attagtcaaa gaagggcgta tagatttctt
     7921 ttgataggag atatacaaca gaaatgaata aatattaacg tgttttcaaa cagtataaaa
     7981 cggtgtggtt tttggaataa aaaaataaca tatctcattt tacacttttg ttattttata
     8041 cttaaaaaat aacgacacag aaaataccgt gtctcagatt tctctactac ttctaaaaac
     8101 tactgttttt ttcctttgaa atgccttcaa aaagtaggat caggcagaat atactggcag
     8161 aatcaaggag aagggagcct aaataaggag aaaaaccata gaaaatataa agtaattact
     8221 tataaaacaa gctttttata gaaaaataat atacctgagt agggtataaa ttaagacgga
     8281 attcctttct agattgagat attgactagg aagaatgtta agattttaag cctttaagct
     8341 aaaaaaaaac cctttttgtt aaataagggg aatatagcct gtcagtttca aactgcaagt
     8401 cgtcctgctg caataatata gtagattaga gatgaaaata gattttttta aaccaatgac
     8461 tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc tattacggtc tttttttacg
     8521 aacgatttta ttgaagacgg acagttcggg tcaaaaataa aaaagctacg atcagaaata
     8581 agaataaaat gtatcccaaa ataatggcta gacggtctaa cttatttgtc gatagatgtt
     8641 tgtttagaag ttgataggcc cataaaccta tcagtcctcc tgttgtatta gttatcaaat
     8701 cagtgatgtc cgctacgcca ataccgaata ggaattgaat caattctgcc aagaagctta
     8761 caaggaaaat aatccctaat tttttagaaa aagaaagtct cttgaaattg acattcaaaa
     8821 gcaccccaaa aggaaaaaag acaatcaagt tataagtggt ctcacgtaat accgtcgtat
     8881 tttcaaaaga aaaagtagaa aaagggacga gattgacatt gctgtaagaa taagtgagaa
     8941 attgtggaag attaaatgat agcttgaata aaatcaacca taatagaaag aataaataaa
     9001 tccctaataa aaattttgat acataccgca tagacatctc ctccatttaa aaagtatacc
     9061 atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt aaggaccaac gtagttatcg
     9121 tacgaaaatt agaagctttt ttttgttggg aatccggttt ctttaacaaa gctaagatag
     9181 cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa tgttcgtttt
     9241 ttgttctaat ttctccaagt tgtttacttt ttgatactaa gtttatgaaa aatcaagaaa
     9301 tagttgttat actaggtaat gctgacaaac gggattggca tttcatcttg gttcagcttt
     9361 ttttccaatt actaggaagg agggatagat tgttcaagac ttattttcgt ttcaataaaa
     9421 tacaagataa tccttttcgt atcatgaatc tgatgctact tatctttatt gtattatggt
     9481 taggttacat aaatcatgat tttttgattt caagtttttc atcattaggg atctttactt
     9541 ttttctatta tcaaaacata ccgatgaaat atttgctgaa acgtatgttt ctgatcggtt
     9601 gcggactgtt ggttgctttc gttttgggga tcttatcgac ttatgtttta tggcttgaac
     9661 catttgccgt agctttggtt gctttttcca gcagattcgt gttgcgatta ttccatattt
     9721 ctaaaccagg tggcttgttt tttgcgatgc tttctgccat gggaacgagc atgcagctgc
     9781 caatcgctca attaccgatc gtctcacttt atttttttat gggtgtcgtt tttgccctga
     9841 ttgcggcagt gattacaaaa ttactggatt cacggcctga gcagacaatt gaaaaagcaa
     9901 ctttaaaaga aagatttcac gaagagcctt tagtgatcat tgattctgtt ttctacagcg
     9961 ctgccttatt tctttccgtt tatgtcagtc acggcttgaa tctgcataat ccttattggt
    10021 tgaccttgtc atgtgcatcg attttgctag cagaaaactt ggatgcgatg aagcatcgac
    10081 aagtccaata tctgattgga tcgatgggtg ggttatgtgt gtcggcattc ttgtcatttg
    10141 tgccgtttac gcagttgcag accatttttc tgatcacttt tctatatgga atcgcacaat
    10201 ttctagttgc tagaaattat gcagttgcta atatttttct taatccgatg gcgttgatgc
    10261 tatctacact tattcgtgga gcatacttga tttctttgat cgaatatcgt tttttaggga
    10321 tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt aatgacggtt ggtttgcaac
    10381 attatttaac ggttgttcgt caaaaattag aatagaagga caaatatatg tgccaaaaga
    10441 ggttatgaca agatttttgt cacaacctct aattcaaggt gttcaaaagc cagattctca
    10501 gcattaagtc aaattcacac aaaaatgtgg aaaggaactt tcaaaaaaat tttatattta
    10561 ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag atggtcaatg
    10621 ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt ttatagttag
    10681 tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat ttaaacaatg
    10741 aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga aatccaaatt
    10801 ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt acaagcaaaa
    10861 aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc taataaagca
    10921 aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc agcattagct
    10981 atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt gaagattcca
    11041 agaactatct atttggcaac aatgaatgct tttactagat ttattcatga acatttccaa
    11101 gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac tagtggcttg
    11161 atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg gtacacggat
    11221 ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc agatttatct
    11281 cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact ttattttcct
    11341 gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa tcttactaaa
    11401 tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt attatcagtt
    11461 gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc ccaaatacat
    11521 gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct tgcaggcgag
    11581 cacattggct ctggcttagg tattcttact tcataagttt agtacttcga aatagtttaa
    11641 aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    11701 cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat
    11761 tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttag
    11821 tgttctatcg aacacacagt acgttcaata ggtaaactag ctataacaat agatattaaa
    11881 gttaacggac aagagggcct tttttaggta gctaaaacgt aataatgaaa atttatttcc
    11941 agagatagga gtcaatcagc tagtctgatc gactgtttta acaagttgac tttcatttta
    12001 acttttgata ccatttaaaa cacaaatcgt aattgtttcg atttgtgttt tttagcatga
    12061 ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa acagatatct caagtgcata
    12121 ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt gcgctgaaga tcatcaaaga
    12181 tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac ttaattaaaa aaatagccga
    12241 aaaacgtttt tcggctattt tcaataatcg gtttttgtgt aaggtttccg aaagaactgc
    12301 tgttgatttt ccatttttaa catatgataa attggtagaa tctttctttt caaattgata
    12361 ccattcccta attccagtta tatagttaac tacagaacca caaagcacgt acgtttataa
    12421 aagtattatc tgtagttatt tttcaattcc ctttcaacct cttattttga ccagtagtat
    12481 tgatcacttt accttttaac acctttgttc gtttttgaaa catttttagt caagaatgga
    12541 aacgtaacta cataatcaaa ggttttgcga aatatttcat gaaatatttc atgtagaagc
    12601 gagcaatacg gttgcaaata accattatcg tttaataagc agttacgaaa gctcccaata
    12661 tctctaacgt tcagctcact ccatttcttt tcttcaaaaa aatcagtgat tcgtatctct
    12721 ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg aaaaaatatg caaaaaaata
    12781 aaaaagcagt tactattgct agaataacat caaggaataa taagttttgg ttttcagata
    12841 atagtcgata agttgtgtat tggaatttta gatacagtaa taaagtattt ttcatgaaat
    12901 tttgtgagaa tcactattac aagtatcttt aataaaatta atggttaagg aggaacttaa
    12961 agtagaatgt aaatgaatag ttgtagtcag tcgattgtga tactagactc aaagttgcag
    13021 gaataagttt ttcaatttgt gattggaaga taaaaaatga aaaagaaggg ctttttttta
    13081 tactttgttg attgtagtgt ttattttccc tctgtttagc caagtaacat tttctatgac
    13141 tcaaccagtt tcaagtgagg aacatgataa cctttttcgt gctacagaag agtttaagaa
    13201 atatcaggaa aatattgttc cggagactcc attagctagt gagccaatta ctgatattga
    13261 tggaaaccaa ataggatggt atatacagta cgaagttatg gtggtaaata atgactcaat
    13321 agtaaaaaat acagtaggct tagagctaac accgattgaa aaggtgagtt cattgttaac
    13381 gtatatttat actgatgaaa gtgacgagac tgagatcatt ttaacggatt actcaaaaat
    13441 tgctactgat aaaaagtata tattgtggat gtagacacta gtgaaatact ttatgatata
    13501 gaaattgatg aaaatttact agatgtagca caagaaatcg atcatcaaaa gcaggaagct
    13561 attcgatatg ctggtagaaa agggaaaaga aaatatttat tacgtgaatg tagcaaaagt
    13621 tagagaagat gaaaatgagt ggaaagaatt caagagtcgc tactcaatta actcgactcc
    13681 gacatttaca gtttacagag aaggatcgat agaaaaaact gttttttgga ctaaagaaag
    13741 tggaatgtct ttagctgaag tagaagagtt tttggattat gttagtatgc aacagtaagg
    13801 gagttcttat tatgaaaaat tttgacacat attatgttat tgttttttcg atatctttgc
    13861 tgttaatatt actaacttta atgcagtata aagagtatcc agagtatttt attttaactt
    13921 ttgtatattt agtaagtagc tacacacttc tatataaatt cagaagttct tctagagttt
    13981 ctaaagtttg tttcttaatt agcttattga ttctataata tttttgattt atataggaaa
    14041 ttaaaaatat tatagaatca acactcagag aacgggttgg tttttaatag atttaataag
    14101 tactgataat gaaatttctc ctggttggga aaacgaagtt ggttaccctg tagaagaatt
    14161 ttttcaagat tactttgcgg agtatgccga taggataatg agagattacg taacagataa
    14221 aactcaagaa gtcatcaatc aatcccagtc attaaatgaa ttatctgata aggcgaaaga
    14281 agaagcagtt gccattattt cttcagggat tagtgactat attgatatga gtggtgaaat
    14341 caattatggc gataaagaca aaattgtacg catgataaaa gcttttatca aaacttttga
    14401 tgtgaaggca gatcttaagt ttaatgatac ttacctagta ggaaaattaa ttaatatcct
    14461 tgggacagag aaagatacgg aaacgtttat ttcctctttg tctatgttgt ttacagataa
    14521 gcatttttca actcagcttg gtggagagga actagtggaa atcttttaac cattacgccc
    14581 agcattaata aaagtatatc aaaaaagtac tcctgatgat ttttaagatt ggtttgagaa
    14641 ataatgtgaa aacactattt ttaatgttaa ttatagatag agatgaacgc gtcatatggt
    14701 taaaagaatg aaaaagtcaa agatacactg ctatatttca ctaaggatat agcagtgtat
    14761 ctttcatgtc gtttcactgg cagtttttta taaaaggagg tgtgcatttc agttgctttt
    14821 ctttggtagt tagttgaatc gtttaagaat taacggatgt ggcagacagg tgctctgtcc
    14881 aaaaaccagt aaaatctttg atggttccat ctttatttag ctgtgcctgt tcaaatactg
    14941 ttttgttttt accgaatgga tcaattgcaa taattgttcc gatccccgca cgtttggcag
    15001 caagtaatcc tgagtaagca tcttcgataa caagacaatc agccggagct aagctcagtt
    15061 tgtctgccgc tttaagaaaa atatccggtt gcggtttccc agggaaagag ccgtcgtcat
    15121 agacaatctt gtcccagtca aaccagcggg ctaaatcgaa aatatcaaaa taaaaagcaa
    15181 cattctcttt gacagtagcc gtggcaatcg ttaatggaat ctgttggtca gtcagctggt
    15241 caagagtcgc ttcaagccct tttgtcaact gaagctcgtt ttcattttgc aagcaaagca
    15301 tacgataata gctttctttt tcttctgaca atacggcgat ctcagaggcg gtcaaatgct
    15361 ctgaaatgaa atgtttcaaa atctccgtat tcgtccgacc atgaatattt tttaaaatat
    15421 cctcgtctga taaaccatct tttgtatgat catgtatcat atgcagccaa gcagcttcat
    15481 gcagatgaga gtccaaaaac attgttccgt tgaaatcaaa tataatccct ttcatagtta
    15541 cgctcctata gtggtatttt cggttgaatc agcaatttgc agatcgacat attgtgcata
    15601 gtgctcttga acctcccgcg agatctggcg atccgtaatg agcagatcga aatcatgcaa
    15661 attataaaac acataaaaat catctcgatt gaatttttta ttatcaacga gtagatattt
    15721 cttacgggaa ttatttagag cgatctgctg tgcttctcct tcttcggtac tgtaagtaga
    15781 gatcgcttcg ttgtgaacgc catttgaact gataaatgct tttgtaaact ttagtcttct
    15841 taaattgtcg ttggtgatcg gtccgacaaa tgcgccggta tttttccgga aatcaccgcc
    15901 aatcagtaga atttctggtc catctgcttg agcaagaata tcaaaaactg gataattatt
    15961 tgtaatgatc cgaattttct tgttcagtaa atgcgtcgcc aatagttcga ttgttgtacc
    16021 tggtcctaag aaaatcgttt ctccctcgtg aatcaaggag gctgctgtgg cagcaatttc
    16081 ttttttttct tgaatctgca gagtggcttt ttcagtgtga gaaagttctt gatctaaagt
    16141 gtaggaaaga ctttgagccc ctccatggac acgcagcagc ttgcccgatt tttctaattc
    16201 atctaaatct ctgcgaacgg tcatatcaga aacatctaat tgattgatga tttcattaac
    16261 ggtaaggatt ccttttttat tgaccatttc tacgatagta agcaatcttt cttttttcaa
    16321 cattgtcttc acctctaatt taaggataat ctatcatata aacggacaac agtcaatcta
    16381 attcgaacaa aagtaaacat ttagggtggt ttttatgttt gttacaatag attttattgc
    16441 tgttttctgg tttttatgat tatttgtgtt tgtttttgtt gacaaatgtt ttggatgcgt
    16501 ttacaatgca atcagttaga aagagcgaaa actgattata gatttgagga gagatgtaca
    16561 tgcttaaatt accagaagat tttatttttg gcggagccac agcagcctat caggttgaag
    16621 gagccactaa ggaaggtggc aaaggcgctg ttgcttggga tgacttttta gaggaacaag
    16681 ggcgattcag tcccgatccg gcaagtgatt tttatcatca atatgcaaag gacattgaat
    16741 tgtgtgaacg ttttggtgta aatggtctaa gattatccat tgcttggagc cgcatttttc
    16801 ctgatggagc tgggaaacca aatccagaag gaatcgcttt ttatcatcgt gtgttcgaag
    16861 aatgtaagaa aagaaacgta acgccgtttg tgacgttgca tcattttgat acccctaaac
    16921 gtttgtttga ccatggagat tttttgaatc gagaaacaat agaagcattt gtttcctatg
    16981 ccattttttg ttttcatgaa ttcaaagaag tcaaggtgtg gagtaccttt aatgaaatct
    17041 atccagttgc aaccaatcaa tatttgttag gtgttttccc accaggaatc aaatatgatt
    17101 ttacaaagat cgtcgcctgt cttcataata tgatggttgc tcatgcccgt gtagtgaact
    17161 atttcaaaga aaacgaactt ccgggtgaaa ttggtgtagt tcactcatta gagacgaaat
    17221 atgcggcaac agacgcgccg gaagataagc atgctgcctt tttagacgat gcattgtcga
    17281 tccgtttctt attagatgct acctatttag gctattattc aactgaaacc ttgactgctc
    17341 ttgatgagat atgtgaagca aatcaggcca gctatcattt tcctgaagaa gatttcgttg
    17401 aattgaaaaa agcatccacg cgtaatgact acttagggat caatcactac caatgccatt
    17461 ttgtcaaagc ttacgatgga gaaaatgcaa tccatcataa cggtacgggt gaaaaaggga
    17521 cctctgttta taaagtgaag ggaatcggag aacgtattta taaagaagga attcctcgaa
    17581 ctgattggga ctggctgatt tatccagaag gtctatatga cttgttgctg cgaattaaaa
    17641 gcgattaccc acattacaac aaaatctata tcactgagaa cgggatgggg tataaagatc
    17701 agtttgaaga tggcattatt atggatcagc cgagaatcga ctatttgaga gtctatttgg
    17761 aatccttaag caaggcgata acagccgggg taaatgtgaa ggggtatttt ttgtggtcgc
    17821 ttatggatct tttctcatgg acaaatgggt ataacaaaag gtatggcttg ttttatgttg
    17881 attttgaaac ccagaagcgt tacccaaaag aatcagctta ctggtacaag ttggtcagtg
    17941 aaaccaaaac gattatttaa taagtgataa tggaggaaga aaagatgaat aaagaggaat
    18001 tacaaatgct tggttttgag atcgttgcat attcaggcga cgctcgcagt acattattga
    18061 aattattgaa agaagtaaga caaggaaact ttgaacatgt tgattcggcg ttgaaagaag
    18121 ccgacgaaaa tttaaccctt gcacataatt cgcaaacaaa aattttagcg gaagaagcat
    18181 ctgggaaaga gatggagatg ggattcattt ttatccatgg gcaggatcat ttaatgacca
    18241 ctttattgct gagagatctg attcaagact ttattgtact gtacagacag aatcaaggat
    18301 aggagaagaa cgctatggat ggaatcatta aacaaatcga aaaagggaaa ccattttttg
    18361 aaaaaatctc acgtaatagt tatttaggag ccgttcgtga cggattttta acagcgatgc
    18421 cagctatttt attttcaagc attttcattt tgattgctgc ggttcctgag atttttggtt
    18481 accaatggcc tgaatctgtc tccacatggc tatggaaagt ctataattat tcgatgggtg
    18541 ttgtagggtt actcgtttca gcaaccactg ctcgctgctt ggctgattcg atgaatcgtc
    18601 gcttaccgaa aaacaaagta atcaacacca cctctgtgat gctggcctct gtggtaagtt
    18661 tcctgttgtt aagcgtctct ccgattgaag gtggattttc aacggaatat atggggacaa
    18721 agggactact ggcttccttt gtcgctgcat ttttaacagt taatttatat aagttttgtg
    18781 tgctgaaaga tattaccatt aaaatgccta aagaagttcc tggaacgatt tcgcaaacct
    18841 tccgagatat ttttcctttc tctttttcag tatttgcggc cgttttaatt gatttaggca
    18901 ttcgacaaat cttccaaatc tcatttgcag aagcaatcat tacggtgctg cagccgctat
    18961 ttacagcagc tgacgggtac ttaggaatcg ctattatctg gggagcgatg gcgctgtttt
    19021 ggtttgtcgg tgttcatgga ccgtcgatcg tggagccggc aattgcggca attatctatg
    19081 ccaatgtgga aaccaatctg caattgttca attccggtga acaagcttct aaagtactga
    19141 cggtgggttt agggaatttt gtaggaacta tgggaggaac tggggcaaca ttggttgttc
    19201 cttttatctt cctactcttt gctaagtcta agcagttgaa ggcagttggg aaagcttcct
    19261 ttattccagt aagtttcgct gtcaacgaac cattgctatt tgcggcaccc atgattttga
    19321 acccttattt ttttattcca tttttgttaa cacctgttgt aaacgtttgc ctgttcaagt
    19381 tttttgtcga tgtattgggc atgaacagct ttatgtacgt tttgccttgg gcaactccag
    19441 caccgatcgg tttggttcta ggaacgggca tgggtatctt agcttttgtt ctagcagcgg
    19501 ttctaatcgt cgttgacttc ctgatctatc taccattttg caaagcatac gatgctgtct
    19561 tagtagagaa agaagcacaa aaagcagagg tgatcgcaac cgaggaaggt gcttctgccg
    19621 atgtacgtga ggaagtacct gtggcagcga gtcaagttat cagcgatgca aataaagaaa
    19681 tcaatgtgtt ggtactttgt gctggcgcag ggacaagtgg gatgttagct aatgctttaa
    19741 aaaaaggtgc atctgaatcg aaagcaccga tcagtgcaat ggcgggtgct tatggttctc
    19801 attacgaaat gctgcaggat tttgatatgg tggtcttagc accgcaagtc aattcttact
    19861 atgatgatat caaagaagac accgatcgct taggaatcaa gctggttgcg acaaaaggag
    19921 cagaatatat cggattaacg agagatcctg aaaaagctgt tcagtttatt ctgaaccaat
    19981 ttcaataaag gaggagccat aatgaaaatg ggaatttggt tgcgtaaagc tgttttattt
    20041 gcagctgtgt taggggcttt gggggctgtt tcaaaaactg ttgaagcatc tgccagcgaa
    20101 tttattcgcg gagcagatat ttcgatcctg tcagatatgg aaaaatctgg tgcaaaatat
    20161 tatgaagacg atatttcaaa agatgcctta gagattttaa aaaataatgg tgtcaactat
    20221 gttcgactgc gtttgtggca ggatccttat aatagtaatg gagaaagtta tggagcaggg
    20281 acaaatgatc tggaaacgac gattgacctt gcaaaacgag caaaaaatct gggacttaag
    20341 gtgctgctgg attttcacta cagtgatttt tgggttgatc ctgggaaaca gaatcttcct
    20401 aaagcatggc aaggtctgac ctttgaagaa atgaatacgg cattgtacga ctatacaaag
    20461 aacgttctgt cggaaatgaa gcagctggac gtttatcctg atatggttca gatcggcaat
    20521 gaactgaata gcggaatgct ctggccttat ggcaagtctt ggggagaagg cgggggagaa
    20581 tttgatcgat tggctgcttt tttgaagtct ggtatccaag ctgttcgaga cactcaacct
    20641 aaaacaactc cagtaatgct ccacttagct gatggtggcg atacaggagc atttacttgg
    20701 tggtttgatg aaatcacgag tcgaggtgtg tcatttgatt taattggagt gtcgtattat
    20761 ccgtattggc acggttcaat gaatgaactt caaaccaata tgaatgcgat cagccaacgg
    20821 tatggtaaag aagtgatcgt agttgaaaca gcttatgggc atacaacagc gaatgccgat
    20881 actatgccaa atgcttttgg tgaagccgaa gcagctgcgg ggggatatga accaacaccg
    20941 acaggacaag cagagtattt gctggatctt gccgatcgga ttcaggcagt accgaataat
    21001 cgcggggcgg gcttcttcta ttgggaacct ctttggtaca acggaaatgt gagttgggca
    21061 acacaagcag gtatgaatta tctcagtgtt caatccacta tgggaaacga atgggacaac
    21121 caagcgatgt ttgattttgc tggaaacgcc ttgccaagct tgcgtgcgtt taaacaagca
    21181 ggggcgcaaa taaatctagt taaaaatgca agtttcgaaa gcgatggacc aacgaccagt
    21241 ccttccaatt ggcaaacctg gtttcaacaa ggaaccgcta agggtacgat caaaacggaa
    21301 gcgggcactt tttttgggga ctacaaacta actttttggc aggatcaagc atacgaggca
    21361 tcagcttatc aaaatgttag tggcataagc aaaggaactt acacattgag tgcctatgta
    21421 atgtctgggg gagagtttga tactaatcag ttatatatca aaaattatgg tggagcggaa
    21481 ctaaatactg cggttgttaa aacaagtgaa tggacaaaaa ttacaatcaa agacattcca
    21541 attacaacag gtgtttgtga aattggggtt tatagcaagg cttcaggaaa tgcttggacg
    21601 aacgttgatt ttgtttcatt ggtgaaacaa tagactgcgg atcgtttcga ttcaagatcg
    21661 gaggttccct tcagccacat cgttagcgta gaaggcagga atcgaacctg cacctcgcct
    21721 ttgccaaaaa taaccagaaa cgatttccag tcatttttac caaaacgagt aaagcaccag
    21781 acttcatcta cactttgtat tatgctaaag agagttcaac gatttcgtaa gaagcaatga
    21841 aaaaattgca aaaagttgtg cagataacat aaagtttgtt taagaaaggg taaccttaga
    21901 attccgtcgt gaatatgatt tgagcactta gatggataga aaaaagttct cggaaaacag
    21961 ttcgtttttc gagaacttct ttttatgata agaatgaaac aaattaactt atattatagt
    22021 ctcttttggt agggaaccat cttattttta atgaaaatca agcattttag ctattgaaga
    22081 atgttgattt atagggattc cccctccata ttatgttggc tctttattcc cactccactg
    22141 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
    22201 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
    22261 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
    22321 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
    22381 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
    22441 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
    22501 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
    22561 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
    22621 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
    22681 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
    22741 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
    22801 cacctttcaa cttgtctaag aagcgatctc ttgcattgac tttgatgatg ttcaagccga
    22861 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
    22921 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
    22981 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
    23041 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
    23101 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
    23161 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
    23221 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
    23281 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
    23341 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
    23401 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
    23461 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
    23521 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
    23581 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
    23641 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
    23701 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
    23761 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
    23821 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
    23881 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
    23941 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
    24001 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
    24061 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
    24121 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
    24181 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
    24241 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
    24301 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
    24361 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
    24421 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
    24481 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
    24541 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
    24601 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
    24661 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
    24721 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
    24781 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
    24841 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
    24901 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
    24961 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
    25021 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
    25081 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
    25141 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
    25201 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
    25261 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
    25321 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
    25381 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
    25441 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
    25501 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
    25561 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
    25621 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
    25681 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
    25741 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
    25801 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
    25861 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
    25921 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
    25981 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
    26041 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
    26101 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
    26161 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
    26221 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
    26281 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
    26341 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    26401 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    26461 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    26521 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    26581 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    26641 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    26701 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    26761 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    26821 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    26881 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    26941 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    27001 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    27061 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    27121 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    27181 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    27241 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    27301 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    27361 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    27421 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    27481 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    27541 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    27601 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    27661 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    27721 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    27781 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    27841 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    27901 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    27961 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    28021 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    28081 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    28141 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    28201 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    28261 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    28321 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    28381 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    28441 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    28501 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    28561 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    28621 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    28681 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    28741 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    28801 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    28861 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    28921 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    28981 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    29041 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    29101 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    29161 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    29221 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    29281 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    29341 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    29401 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    29461 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    29521 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    29581 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    29641 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    29701 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    29761 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    29821 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    29881 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    29941 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    30001 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    30061 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    30121 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    30181 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    30241 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    30301 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    30361 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    30421 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    30481 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    30541 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    30601 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    30661 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    30721 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    30781 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    30841 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    30901 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    30961 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    31021 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    31081 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    31141 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    31201 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    31261 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    31321 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    31381 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    31441 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    31501 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    31561 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    31621 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    31681 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    31741 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    31801 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    31861 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    31921 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    31981 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    32041 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    32101 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    32161 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    32221 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    32281 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    32341 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    32401 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    32461 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    32521 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    32581 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    32641 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    32701 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    32761 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    32821 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    32881 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    32941 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    33001 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    33061 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    33121 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    33181 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    33241 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    33301 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    33361 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    33421 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    33481 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    33541 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    33601 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    33661 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    33721 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    33781 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    33841 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    33901 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    33961 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    34021 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    34081 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    34141 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    34201 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    34261 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    34321 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    34381 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    34441 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    34501 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    34561 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    34621 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    34681 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    34741 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    34801 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    34861 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    34921 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    34981 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    35041 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    35101 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    35161 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    35221 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    35281 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    35341 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    35401 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    35461 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    35521 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    35581 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    35641 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    35701 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    35761 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    35821 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    35881 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    35941 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    36001 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    36061 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    36121 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    36181 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    36241 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    36301 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    36361 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    36421 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    36481 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    36541 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    36601 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    36661 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    36721 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    36781 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    36841 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    36901 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    36961 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    37021 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    37081 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    37141 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    37201 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    37261 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    37321 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag
    37381 cttttcatgt ttgatgaata ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt
    37441 tatacgaaaa aagggactgt gacaaatcgt gtcacagtcc cttttttata gtgtttttta
    37501 taggtagtat tttttagcaa ctgttaagtc atcatttaat tcataaacaa gtggttgacc
    37561 tgttggaatt tcaagatcca tgatgtcttc gtcagagata ccttcgatat gtttagctag
    37621 ggcgcgtaat gagttaccat gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg
    37681 agcaatttca tcttgccaga atggcaaagc gcgttctaat gtcactttca agttttctcc
    37741 accaggaata tcgcgtttgt ctaacattgc gtaacgacgg tcatttgctg cagaaccttc
    37801 gtcagtttct tccattaatg gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg
    37861 ttcgtcgccg tatttttcag ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg
    37921 acgttcgttt aagcgccatg atttgatttg tggtacccaa agttgatctg aatattctaa
    37981 gatcaagtta catgttttga ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc
    38041 gattccagct tctttgattt tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc
    38101 tagatttaca tcagcccaac cagtaaattg gttcaatgca ttccattcac taagaccgtg
    38161 acgagaaaaa actaattttg gcatgtttag ctctcctttt aagataggtt tatatttctg
    38221 aaattttcag ttctgtttta ttgtacaatt ttttggaaga gatttccatc gaaaaattag
    38281 ataagattct atgagaaaac aagtagagaa tataaaaaaa cgaacagtgc ccacgcaact
    38341 gttcgttttt aggtattaga tgcataattg ctcgatttct ttttcaggta aattgatata
    38401 tttataacat gttccaactg aaacaccgca ttttgaagaa ataaattgga tcgtttcttt
    38461 tttttcataa tagagacgtc ggattttttt gatcgttttt gcatcgattt ttggtcttcc
    38521 gccgattttt ccttttttac gagcttcatc taatccaact aatgtacgtt ctttgatcaa
    38581 tgcgcactcc atgtttgcta ggttttccat taattggaaa tagatttttc ccatttcgcg
    38641 cgtatcgatt ccttcatcaa gactaatcaa gtgcagcccg cgatctttga ataattgcgt
    38701 gagttctgtt aattgccgcg tagatttgcc taatcgatga agttggcaaa tgactagtgc
    38761 atcgccttgt tgcatgtcag cgatcactga ctccaaatga acagcagtag aatcattttg
    38821 ttccacttca tatgtctcat ggaaaatttc ttgacaacca aagtcagata attgatcaac
    38881 ctgagttgtt aaatcaacgt catttatggt tgtgcgtgca taaccaataa ttttcatggt
    38941 gattcctttc tagcagtgat tttctaactg gatataacag taaaaatgta gcataaagta
    39001 cgtcaaaaag atagtttttt cgcaaatttt ccgctgtctt tttttattgg atgatcaagc
    39061 agtttttctt tatttacgac ggattgcgtc aagaatctca acgccttttt ctgagccgac
    39121 aactacttta gaaaccatgt ttaggaaaag cccatgttct actaccccaa caagttcatc
    39181 taaataggta gctaagcttt ctggctgttc gatgacttct agatgcagat caatgatgta
    39241 atgaccacca tctgtggtta agacttctcc gttttcgtct gtacgcaaga cgggttggaa
    39301 tcttttttct tcaaaaatat gaagtagctg ttgggagcca taagggatca cttcaactgg
    39361 gagtgggaac ttcccaagtt tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc
    39421 ttttgagtac gtagcgacta ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc
    39481 ttgaaaatca gcactgattt catcagcacc gtctattgtc aaatcgacta cggggacttc
    39541 atcaatactc tttaacggta ttcctaattt ttccgcttgt tcttttgtcg catttgaagt
    39601 agtaactcct gtgatacgta aaccttcttc acgcatacgg cgaccaatct cttctaccat
    39661 ataataggcg gttgaaccgg tcccgagacc tacgatcatc ccgtccttta cgaatttcgc
    39721 ggcttcaatg ccagctaatt gttttaaatt catctttgcg tccacctttt tttagtttga
    39781 gcatcgtgct ttcctcacta ttttagcgtg atttacggta aaaagatacc attctttttt
    39841 attccaataa agctttttca aaaaaaattg catctcttgt tgacatgttt tttcattcgt
    39901 gatattctaa caaaggtgct ttatctacag gtttctgtga agaaacgtgc tgactgttta
    39961 tcaaagcata tttgataagg atgcagacat tgcatttatt ttttatcaca aaaaaagaac
    40021 cacctggatg tgtggaacta gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca
    40081 attaaccaat tagtacgtaa acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct
    40141 ttgaacaaag gatataacag ctttaagaaa actcaaacta acgtaaactc acctcaaaaa
    40201 cgtggggtag caactcgtgt gggtacaatg acacctaaaa aaccgaactc agctttacgt
    40261 aaatatgcac gtgttcgttt gtcaaactta attgaagtta ctgcttatat cccagggatt
    40321 ggtcataact tacaagaaca cagtgttgta ttattacgcg gtggtcgtgt aaaagactta
    40381 ccaggggtac gttaccatat cgttcgcggt gctttagata ctgctggtgt taacgaccgt
    40441 aaacaaagcc gttctaaata cggaactaaa aaacctaaag cttaatttta attattaaac
    40501 aaatgaaact tgcttctact atatagaagt aattactatg aacaatattt cggaaggagg
    40561 agttacggat gccacgtaaa ggtcctgttg caaaacgtga tgttttacca gatcctattt
    40621 ataactcaaa attagtaact cgcttgatca accgtgtaat ggttgacgga aaacgcggga
    40681 ttgcagctaa cattatctat aatgcctttg acttgatcaa agaatctaca ggtaacgatc
    40741 cattagaagt gtttgaacaa gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac
    40801 gtcgtgttgg gggttcaaac taccaagtac cagttgaagt tcgccctgaa cgccgcgtta
    40861 ctctaggtct tcgttgggta gtaaactacg ctcgcttgcg cggtgaacac acaatggaag
    40921 aacgcttagc aaaagaaatc atggatgctg caaacaacac aggtgcttct gttaaaaaac
    40981 gtgaagacac tcataaaatg gctgatgcca accgtgcgtt tgctcactat cgctggtaag
    41041 attctctctg tttggtcggc ttttataagt cgacctcaga tagtgtcctt atttttagca
    41101 actagatagt aaagagagga gaaacaagga gaatgaccag agaattttct ttacaaaaca
    41161 ctcgtaatat cggtatcatg gcccacatcg atgctggtaa aacgactgct actgaacgta
    41221 tcttgtacta tactggtaaa atccataaaa ttggtgaaac tcatgaagga gcttcacaaa
    41281 tggactggat ggaacaagaa caagaacgtg gtattacgat cacttctgct gctactacag
    41341 cacaatggaa gggtcaccga atcaacatca tcgacacacc aggacacgta gacttcacag
    41401 ttgaagttga acgttcattg cgtgtacttg atggtgctgt aactgttctt gatgcacaat
    41461 caggtgttga acctcaaact gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc
    41521 gtatcgtatt tgcaaacaaa atggacaaga tcggtgctga cttcttatat tcagtatcaa
    41581 ctttacatga ccgtttgcaa gcaaacgctc acccaatcca attaccaatt ggtgcggaag
    41641 acgacttcac aggaattatc gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc
    41701 ttggaacaga gatcgaagaa acagaaattc ctgaagaata ccgcgaatta gctgaagaat
    41761 ggcgcgaaaa attggttgaa gctgtagctg aaactgatga agaacttact ttgaaatact
    41821 tagaaggtga agaaatcact gaagctgagt taaaagaagg tatccgccgc gcaacagtta
    41881 acgtagaatt ctacccagta ctatgtggtt cagcatttaa gaacaaaggg gttcaattac
    41941 tattggacgc tgttcttgat tacttgccat caccgttaga tatcccagct atcaaaggga
    42001 ttgaccctaa aactgatgaa gaagtagaac gtcctgcaga cgacagcgct cctttctcag
    42061 ctttagcatt taaagttatg actgacccat tcgtaggtcg tttaactttc ttccgtgtgt
    42121 actctggtgt tttacaatca ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac
    42181 gtgttggtcg tatcctacag atgcatgcca actctcgttc agaaatctct gaagtttacg
    42241 ctggagatat cgctgctgct gttggtttga aagacacaac tactggggac acactatgtg
    42301 atgaaaagag tctagttatt cttgaatcca tggaattccc agaaccagtt atccaagtcg
    42361 ctattgaacc taaatcaaaa gctgaccaag ataaaatggg tgtggcttta caaaaactat
    42421 ctgaagaaga tcctactttc cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg
    42481 ctggtatggg tgaacttcac ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag
    42541 ttgaagctaa cgtaggtgcg cctcaagtat cttaccgtga aactttccgt gctggcactc
    42601 aagccgaagg taagtttgta cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga
    42661 ttgaatttac accaaatgaa gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg
    42721 gggttgttcc tcgtgaatac attccagctg ttgaaactgg tttgaaagat gctatggaaa
    42781 atggtgtctt agctggctat ccattagtag atatcaaagc taaactttac gatggttcat
    42841 accatgatgt cgattcaaat gaaactgcct tccgtgttgc tgcttctatg gcattacgtg
    42901 cagcagctaa gaaagctaat ccagtaatcc ttgaaccaat catggctgta gaagttgtta
    42961 tccctgaaga ttatttaggt gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg
    43021 aaggtatgga agctcgcgct ggcggccaac aagttgttcg tgcgatggtt ccacttgctg
    43081 aaatgtttgg ttatgctaca actcttcgtt ctgctactca aggtcgtggt acatttacaa
    43141 tgacatttga ccactacgaa gatgtaccaa aatcagtaca agaagaaatc attaagaaaa
    43201 acggcggaaa agctgaataa tttcttgaaa ccggatagtc ctgcaaagag attatccaaa
    43261 gagactatat ttttaatata gtttcgtgta aaataattat cgatgatatg ggcatgtggt
    43321 gtaccttgtg tacatcatat acctatcaga caaataaaaa atatattgta tttttaggag
    43381 gacattttaa aatggcaaaa gaaaaatttg accgttctaa accacacgtt aacattggta
    43441 ctatcggaca cgttgaccat ggtaaaacta cattaacagc tgcaatcaca actgtactat
    43501 ctaagaaaaa cggcggccaa gctatggctt acgatcaaat cgatggtgct ccagaagaac
    43561 gcgaacgcgg aatcacaatc tcaacagctc acgttgagta tgaaacagat acacgccact
    43621 acgctcacgt tgactgccca ggacacgcgg attacgttaa aaacatgatc actggtgctg
    43681 ctcaaatgga cggagctatc ttggtagttt ctgctgctga cggcccaatg cctcaaactc
    43741 gtgaacacat cctattgtct cgtcaagttg gtgttcctta catcgttgta ttcttgaaca
    43801 aagtagacat ggttgatgac gaagaattac tagaattagt tgaaatggaa gttcgtgacc
    43861 tattaacaga atacgaattc cctggtgacg atgttcctgt agttgctgga tcagctttga
    43921 aagctctaga aggcgacgct tcatacgaag aaaaaattct tgaattaatg gctgcagttg
    43981 acgaatacat cccaactcca gaacgtgaca acgacaaacc attcatgatg ccagttgaag
    44041 acgtgttctc aattactgga cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag
    44101 ttcgcgttgg tgacgaagtt gaagttgttg gtattgctga agaaacttca aaaacaacag
    44161 ttactggtgt tgaaatgttc cgtaaattgt tagactacgc tgaagctgga gacaacattg
    44221 gtgctttact acgtggtgtt gcacgtgaag acatccaacg tggacaagtt ttagctaaac
    44281 caggtacaat cacacctcat acaaaattct ctgcagaagt atacgtgttg acaaaagaag
    44341 aaggtggacg tcatactcca ttcttcacta actaccgtcc acaattctac ttccgtacaa
    44401 ctgacgtaac aggtgttgtt gaattaccag aaggaactga aatggttatg cctggcgaca
    44461 acgtaacaat ggaagttgaa ttaatccacc caatcgctat cgaaaacggt actaaattct
    44521 caatccgtga aggcggacgt acagttggtg ccggtgtcgt tacagaaatc aaagcttaat
    44581 tcacttaagt tttaaagtaa agcaacattt attcggacgt aatcgctcgg aacgaaggtc
    44641 ttaggatctt tgttccgagc ttttttattt attgcaggat caggaaaaaa gacaggagta
    44701 ttataaatag aagaaacacg aaataaaaga gtcagtattt tctaaatgaa actttatgaa
    44761 aacaaaatgt tttttacaag aaaaagcctt gctttgtgag tttatatata ttataatgac
    44821 aaaggtgcgt ttactttagt gaattgcaca gacgtaatca gaatgatttc gtcggctaag
    44881 aaacgagagg ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac ggaaattttc
    44941 gtggagctat gtctactttt caaaataggc gaaggaggga acaagatggc aaaacaaaaa
    45001 attcgtatcc gtttaaaagc gtatgaacac cgtattttag atcaatcagc ggataaaatc
    45061 gtggaaactg caaaaagaac tggagctagc gtgtctggtc caattccatt accaactgaa
    45121 cgcagtcttt acacaatcat ccgtgcgact cataaataca aagattcacg cgaacaattc
    45181 gaaatgcgta cacacaaacg tcttatcgac attgtgaacc caacaccaaa aactgttgat
    45241 gctttgatga agctagactt accatctggt gtaaacatcg aaatcaaatt ataaaactaa
    45301 aacaaaaata atggaggtgt actcatgacc aaaggaatct tagggaaaaa agtgggaatg
    45361 acacaaatct tcactgaatc tggtgaatta attccagtaa ctgttgtaga agctactcca
    45421 aacgtagtac tacaagtgaa aacaatggaa acggatggct acgaagctat tcaagttggt
    45481 taccaagata tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca
    45541 aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa
    45601 gtaggaaaag aaattacagt agatgttttc caagcaggag acattattga tgtaacaggt
    45661 actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag ccgcggacca
    45721 atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac
    45781 cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa
    45841 aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt
    45901 cctggagcta aaaaatcttt aattactatc aaatcagctg tgaaagctaa ataattgtag
    45961 gaagaaagga ggaactaagg aatgccgaat gtagcattat tcaaacaaga tggaagccaa
    46021 aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg aacctaatga aagtgttgtt
    46081 tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac
    46141 cgtagcgctg ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca
    46201 cgtcaaggat ctatccgctc accacaatgg cgtggaggtg gcgtagtctt tggaccaaca
    46261 ccacgttctt acagctacaa acttcctaaa aaagttcgtc gtttagcaat gaaatctgta
    46321 ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca
    46381 ccaaaaacaa aagaattcaa acaagttctt gctaacttat ctatcgactc taaagtattg
    46441 gttgttctag aaaaaggtaa cgactttgct gctttatcag cacgtaactt accaaacgtt
    46501 tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta
    46561 gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg cataatgaac ttactagacg
    46621 taatcaaacg cccagtgatc actgaaaaat caatgcttgc catggatgac aagaaataca
    46681 cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt
    46741 ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg
    46801 gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat cgttacattg actgaagatt
    46861 caaaagaaat ccaattattc gaagctgctg aataagcact aatagataac tcattaaatt
    46921 aggagggaaa aacgtggcga ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat
    46981 gacaggttct gattttgctg aaatcacaac atcaacacct gaaaaatcat tgttacaacc
    47041 attgaaaaac catgctggac gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg
    47101 cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt aacaaagata acgttgttgc
    47161 aactgttcaa acgatcgaat atgatccaaa ccgttcagct aacatcgcgt tagtacacta
    47221 tgaagatgga gtgaaagctt acatcttagc acctaaagga ctagaagtgg gtatgaaact
    47281 tgtttcaggt ccagaagcag atatcaaagt cggaaatgct cttccattag aaaacattcc
    47341 agttggtact gttatccaca acattgaaat gaaacctggt aaaggtggac aattgatccg
    47401 ttctgctggt acaagtgctc aagtacttgg taaagaaggc aaatacgtat tgatccgctt
    47461 gaactctggt gaagttcgta tgatcttagc tgcatgccgt gctacaatcg gttctgtagg
    47521 taacgaacaa cacgaattaa tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa
    47581 acgcccaact gtacgtggta gcgtaatgaa ccctaatgat cacccacacg gtggtggtga
    47641 aggtaaagct cctatcggac gtaaagcacc agtttctcct tggggtcaac cagctcttgg
    47701 ttacaaaact cgtaacaaaa aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa
    47761 taaataatta ctttgaccat gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag
    47821 tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg
    47881 ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt
    47941 tgttggatat accatcgcag tttatgatgg acgtaaacat gtaccagttt acatccaaga
    48001 agacatggta ggacacaaat taggtgaatt tgcacctaca agaacttatc gtggacatgc
    48061 tgctgacgat aagaaaacga aacgctaatt tgaggggagg ataaatccaa atggcagaac
    48121 aaatcacatc agctaaagca actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt
    48181 tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca
    48241 caccaaacaa atctgctgga atcattgaaa aagtattgat gtcagcagtt gcgaatgcag
    48301 aaaataactt tgacttagat gttgaaaact tggtagtaag cgaagctttt gttaacgaag
    48361 gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca atcaacaaac
    48421 gtacaagtca tatcacggta gtcgtatcag aaaaataagg agggataatc agtgggtcaa
    48481 aaagtacatc caattggaat gcgtgtaggc atcatccgcg actgggatgc taaatggtat
    48541 gctgaaaaag agtatgctga gttcttacac gaagatttaa gaatccgtaa atttatttca
    48601 actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta
    48661 aacatttcaa ttcacacagc taaaccaggt atggttattg gtaaaggcgg ttctgaagtc
    48721 gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa gaatccatat caatatcgtg
    48781 gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta
    48841 gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct
    48901 ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt
    48961 tcagaaggat actcagaagg aactgttcct cttcatactt tacgtgcgga cattgattac
    49021 gcatgggaag aagcagatac aacatacgga aaactaggag ttaaagtgtg gatctatcgt
    49081 ggagaaattc ttccaactaa aaaaaacact gagaaaggag ggaaataatc atgttagtac
    49141 ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg
    49201 gaaaagaagt agcattcggt gaatacggtt tgcaagctgt tgattcacat tggatcacaa
    49261 accgccaaat cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag
    49321 tatggattaa aattttccct cacaaatctt atactgccaa agcaattggg gtacgtatgg
    49381 gttctggtaa aggggcacct gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt
    49441 ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca
    49501 aattaccaat gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag
    49561 gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa
    49621 gaagaattgt ttaatcttag attccaatta gcaacaggtc aattagaaaa cactgcacgt
    49681 attaaagaag tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga acaagctaag
    49741 taattatgga aggaggccat taagcgtatg actgaaggaa gaaatcaacg caaagtttat
    49801 caaggtcgcg tggtttcaga caaaatggat aaaacaatta ccgttgctgt cgaaacaaag
    49861 aaaaaccacc ctatctatgg caaacgcatg aactattcta agaaatacaa agtgcatgat
    49921 gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta tggaaactcg tccattatca
    49981 gctacaaaac gtttccgttt gttggaagta gtcgaagaag cagttattat ctaataacac
    50041 gagattttcc tatatagatt gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa
    50101 gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa
    50161 gtccttggtg gatctggtcg taaaactgct aacatcggtg atgtgatcgt tgctacggtt
    50221 aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt
    50281 cgaacaaaat caggagctcg tcgtacagac ggttcatata ttaaatttga tgaaaatgct
    50341 gcagtgatta ttcgtgatga taaaagccct cgtggaacac gtatctttgg gccagttgca
    50401 cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga agttttataa
    50461 tcttgagaca cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa
    50521 gttaaagtta tcactggaaa agacaaaaac aaagaaggcg ttgtactagc agcgttcccg
    50581 aaaaaagata aagttattgt cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct
    50641 caagctgctc ctcaaggtgg aattgttgag atggaagcgc caattcatgt ttctaacgtg
    50701 atggtggttg actctactgg tgtagctggc cgagttggct acaaagaagt agacggcaaa
    50761 aaagtccgtg tttctaaaaa aaccggtgaa gttttagata aataaaagtt taaggaagga
    50821 gggtcttact gaatgaaccg cctaaaagaa aaatatctta aagaagtaac tccatcattg
    50881 atggaaaaat ttaactatag ctctgttatg caaacaccta aagttgaaaa aatcgtcatc
    50941 aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact tagataaagc agttgaagaa
    51001 ttaactttga tcactggtca aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga
    51061 ttccgtttac gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg agaaagaatg
    51121 tacgaattct tagacaaatt ggtttctgtt tcactaccac gtgtacgtga cttccatggt
    51181 gtaagtaaaa aagcctttga cggtcgtgga aactatactt taggtattaa agaacaattg
    51241 atcttcccag aagtagatta tgatttagta gataaagtac gtggtatgga catcgttatt
    51301 gtaacaacag caaactctga tgaagaatct cgtgagttgt tgacacaatt aggcatgcca
    51361 ttccaaaaat aattaaagga ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac
    51421 aaacgtcctg caaaattttc aacacaagct tatactcgtt gcgaacgttg cggacgtcca
    51481 cattcagttt atcgtaagtt ccatctttgc cgtatttgct tccgcgaact tgcctataaa
    51541 ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca taagaaggag
    51601 gtaaaagatc aatggtcatg acagatccaa ttgcagattt tctaactcgt atccgtaacg
    51661 caaacatggt taaacatgag tctttagaag tacctgcatc aaaaattaaa cgtgacatcg
    51721 ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa gatgacaaac
    51781 aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact
    51841 taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag
    51901 tattgaacgg tttaggtatc gcaatcattt cgacttctga aggtgttgtc actgataaag
    51961 aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa
    52021 tctacaagga ggtgtcttta agtgagccgt atcggaaata aagtcgttgt tatccctgaa
    52081 ggagtaacag ttactcaaga cggaaacaac gttacagtta aaggaccaaa aggtgaattg
    52141 actcgtactt tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt aactttcact
    52201 cgtccaaacg acagtaaaga aatgaaaaca attcatggaa caactcgtgc gaacttcaac
    52261 aacatggttg taggtgtcag cgaaggtttc caaaaagcgt tagaactaat cggggttggg
    52321 taccgtgcac aattacaagg atcaaaactt gtattgaacg ttggttactc acatccagta
    52381 gaaatcacac caccagctgg agtaactgtt gaagtaccag ctaacacaca agtaattgtt
    52441 aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa acattcgtgg aactcgtcct
    52501 ccagaacctt ataaaggtaa aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa
    52561 ggtaaaactg gtaaataatc gtttagaaaa attgcataga agccagcagc aggaaaaagg
    52621 catttggctg aagtttacct tcaaaaaaat acatttttac ttgtgccgca gcttctattt
    52681 ttcgaaacgt atttactgct tgaaatagta gtacaataaa aataaagagg tgacaattgt
    52741 gattacaaaa ccagataaga acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa
    52801 tatctctggt actgctgagc gcccacgctt gaacgttttt cgttctaaca aaaacatcta
    52861 cgctcaaatt attgatgacg tagcgggtgt aacgctagca agtgcctcta ccttggataa
    52921 agaaatttca ggtggaacaa aaacagaagc agcacaagct gtcggtaaac tagttgctga
    52981 acgtgcagct gaaaaaggca ttaaagtagt agtctttgac cgtggtggat acctttacca
    53041 tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa
    53101 ggaggaatac cattcatggt ttatatcgat ccaaaacatt tggaattaga agaccgcgtt
    53161 gttgcaatta accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct
    53221 ttagttgttg tcggtgacaa aaacggacac gtaggatttg gtacaggtaa agctcaagaa
    53281 gttcctgaag caatccgtaa agcaattgaa gatgcgaaga aaaacttagt tgaagtacct
    53341 atggttggtt caacaatccc acacgaagta atcggtgctt ttagtggtgg tagaatccta
    53401 atgaaacctg ctgtagaagg ttctggggtt gctgctggtg gaccagttcg tgccgtatta
    53461 gaattagctg gggtagcaga tatcacttct aaatctttag gctcaaatac accaatcaac
    53521 gttgttcgcg caacagttga aggtttgaaa caattgaaac gtgcagaaga agttgcagaa
    53581 cttcgcggca aatcagttga agaattaatc ggataaggag gacaacaata atggctgaat
    53641 taaaagttac tttaaaacgc agtattatcg gacgtcctca aaaccaacgc gatacagtta
    53701 aagcgttagg tcttacaaag attaacagtt cagtggttaa gccttctaat gaagcaatta
    53761 aaggcatgat caatactgtt tctcacctag tggacgttga agaaatttaa tttagaaaaa
    53821 tcataactag aacattaagg aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct
    53881 gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa
    53941 actgccggac gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga
    54001 tttgaagggg gacaaacacc attgttccgt cgtttaccaa aacgaggatt cacaaacgtg
    54061 aaccgtaaag actatgcagt cattaatctt gatgtattga accgctttga agacggagct
    54121 gaagtaactc ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa agctggaatc
    54181 aaagtattag ctaatgggga actaaacaag aaattaactg tgaaagcagc aaaattctct
    54241 aaagcagctc aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta
    54301 gactattaaa agatgctttt aaagtaaaag acattagatc gaaaatccta tttactgtat
    54361 ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt accaggcgta aatgctgctt
    54421 cattgcaaaa cctttcttct ttgccatttt tcaacatgtt gaacttggta agtggtagtg
    54481 cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc atacattact gcttctatcg
    54541 tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg
    54601 aagttggtcg taaaaaactg aatcaagcga ctcgatatct gacattagta ttggcattta
    54661 tccaatctgt cggtatcact gctggattca atcaatacgc tcaattaggt ttcgtgaata
    54721 atcctaacgt agcaacttac gtaacgatcg gtttgattct aacagccggt acgatgtttg
    54781 ttacttggct aggagaacag atcacagaaa aaggtatcgg aaatggcgta tcaatgatca
    54841 tttttgcagg tatcatttct agattgcctg aaagtatcag agaaatttat gaagattact
    54901 ttataaatat tgatccttca gacatatgga aatctgctat ttttgtggcc attttactag
    54961 tagcggtctt gttgatcgtg acattcgtaa catttttcca acaagctgaa cgcaagatcc
    55021 cgatccaata tacgaaacgc gtagctggcg cgcctacaag cagctatctg ccgttgaaag
    55081 taaatgcagc tggagttatt ccagttatct ttgctagttc gtttattgct acgccaaatg
    55141 ctgttttaca agcattgagc ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg
    55201 agatctttaa ctataataca gcacctggtg ctacgattta tacgattctg atcgttgcct
    55261 tcactttctt ctacgccttt gttcaagtaa accctgagaa attagcggaa aacttgcaaa
    55321 agcaaggaag ttatatacca agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa
    55381 gattactgat gcgtttaagt acagttggtg cagtcttcct tggtttagtg gcattgcttc
    55441 caatcattgc tcaaatggtt tgggatcttc cacaatcaat cggattaggc ggtacaagct
    55501 tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga
    55561 aacgtaaata tgttggattc attgagtaag aaagcgtgtt gaggtttccc ctcaacacac
    55621 gttctcacac atttattagg agggaaaaca atgaacctca ttttaatggg gctgccaggc
    55681 gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt atggtattcc gcacatctca
    55741 acaggagata tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag
    55801 tcttatatgg ataaaggtgc attagttcct gatgaagtaa caaatggaat cgtgaaagag
    55861 cgactagctg aaccagatac agagaaaggc ttcttattag atggctttcc acgtacgcta
    55921 gatcaagcaa aagctctgga cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc
    55981 atcgatatcc atgttggtga agaaatcttg gtcgaacgtc tagccggccg atttatctgc
    56041 agcaattgtg gagcaactta ccataaaatt ttcaacccta caaaagttga agacacatgt
    56101 gatcgttgcg gtgggcatga attctatcaa agagaagatg ataaacctga gacggttaaa
    56161 aatcgtctgg ctatcaacat caaaaatagt gaaccaatct tggcttatta taaagaacaa
    56221 ggtttgctaa acactattga tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa
    56281 aaaatcattg aaaaatagca cgattgcccg tagccgactt gcattatgca gttactcttg
    56341 tcaagttcaa taaattatga tagaatatgt cagtccgact tctagagaat cctctagatg
    56401 atccattttg agaattttga ggtgggaact gcagtttccg ccattttatc gtgtgaaaat
    56461 gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg
    56521 tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa acggacacca
    56581 agttcttgct actgtttcag gtaaaattcg tatgcattac atccgtatct tacctggtga
    56641 caaagtaacg gttgaattgt ctccatacga tcttaaccgt ggccgtatta cttatcgctt
    56701 taaataattg tactccgtaa aactacagga ggtattatca tgaaagtaag accatcagta
    56761 aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc
    56821 ccagcaaatc caaaacataa acaacgtcaa ggataattgg aggtgctata aatatggctc
    56881 gtattgcagg agtagatatt cctcgtgata aacgtgtagt agtttcactt acttatatct
    56941 atggtattgg taacactact gctaaaaaga tcttagctga tgctggtgta tctgaagatg
    57001 ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa gtagataaat
    57061 taaaagttga aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa
    57121 tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga
    57181 aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat cgctggcaag aaaaaataat
    57241 tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg
    57301 tgaagaagaa tatagaagca ggtattgcac atatccattc tacattcaat aacactatca
    57361 tcatgatcac tgatactcac ggaaatgcat tagcgtggtc atcagctggt tcattaggtt
    57421 tcaaaggaag caaaaaatca actccttttg ccgctcaaat ggcagcagaa actgctacaa
    57481 aagcagcaat ggaacacgga ttaaagactg ttgaagtaac agttaaagga cctggttctg
    57541 gacgtgaagc agcaattcgt tcattacaag ctacaggttt agaagtgact gcaattcgtg
    57601 acgtgactcc agttcctcat aatggtgccc gccctccaaa acgccgtcgt gtttaatgag
    57661 tgccgctcat tttgagtttt atcagtaacg tcattgaaca agacactttt cgttttgaaa
    57721 ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa
    57781 aaagattatg gcaagttcat cgttgaacct cttgaaagag ggtatgggac tactttaggg
    57841 aattccctac gtcgtatttt attatcttct ttgccaggtg ctgccatcac taatattcaa
    57901 atcgatggtg tactgcatga attctctacc atcaaaggtg ttagagaaga cgtcactcaa
    57961 atcatcttga acattaaagg cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt
    58021 gaaatcgata ttaccggacc tgctacagta actgctggcg atattatcgt tgatagtgat
    58081 gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc tacgttccat
    58141 gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa
    58201 gatatgccaa ttggcgttct tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac
    58261 tatcaagttg agaacacacg tgttggacac cgtgaagatt ttgataaatt gacgatggag
    58321 atttggacag atggttcaat tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg
    58381 actgaacatt tggatatttt tgtgaactta actgatgaag caaaaaatgc tgaaatcatg
    58441 gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga agagttagac
    58501 ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt
    58561 acaaataaat ctgagcctga aatgatcaaa gtccgtaact taggacgtaa atcattagaa
    58621 gaagttaaag caaaattgca cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt
    58681 ttacgaagga gggaaaccaa cgtgagttat cgtaaactag gacgcacatc tagccaacgt
    58741 aaagcgatgt tgcgtgattt aacaactgat ttattaatca acgaacgtat tgtgactact
    58801 gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc
    58861 ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg
    58921 cgtgaagaaa atgaggacat cgtcattgaa tcagctttac aaaaactgtt caacgatatt
    58981 gcacctcgtt acgcagaacg tcaaggcgga tacacacgca ttttgaagac agaaccaaga
    59041 cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct aaaatcacac taataacatc
    59101 actttataga tgattctttt agagtgttat gatgttggaa gatatttttc cgagtctagc
    59161 tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag cactcatcat aaagtgtttt
    59221 tttatacctt caattacctg tcatgacaga tatgtttgaa ggcataaaaa aacattttaa
    59281 gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac tttaggatga
    59341 agaaggagta atcatggagc caattattga attagaaaag ataaattata aataccagcc
    59401 agatgatctt cgccccgcat tgaaagatgt ctcctttaca attgataaag gtgagtggat
    59461 cgctattatt ggacacaatg gttcaggaaa atcgacactt gccaaaacga tcaatggatt
    59521 gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa atattggatg aagaaaatat
    59581 ttggacgatc cgacagatgg ttggaatggt ttttcaaaat ccagacaatc agtttgttgg
    59641 ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat caagggattc ccagagaaga
    59701 aatgctagtg cgtgtgaaag atgcacttga gaaagttaga atggctgagt ttgcttcacg
    59761 cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc
    59821 cttacgacct gatatcatta ttttagatga agcaaccagt atgctagatc cagaaggtcg
    59881 tgaagaagtg atctctacga tcaagaagat caaagaagaa agtcaattga ccgttatatc
    59941 tatcactcat gatattgatg aagcagctaa tgccaatcgg atactagtga tgagacaagg
    60001 cgaacttgtc cgtgaaggta caccaaaaga aattttttct gctggtcctg aactgatcga
    60061 cttaggtcta gatctaccat ttcctgaaaa actgaagagc gcactgaaag aacgtggggt
    60121 tgatgtgccc agtgaatata tgacagaaga aaggatggtg gattggttat ggacatccgt
    60181 tttgaacaag tagacttcac ttatcagccg aatacaccat ttgaacaaag agccttattt
    60241 gacatcaata tgacgatcaa agaaaacagt tatactgcat tagtagggca taccggaagc
    60301 gggaaatcaa ccttacttca gcatttgaat gcgcttgtta agccaacaag cggtacagtc
    60361 catattggag aacgagatat tcagccggat acggataata agaatttaaa gcccatccga
    60421 aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc tattcgaaga aacggtagca
    60481 aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc actagtccta
    60541 gcaaaagaaa cattagaaca agttgggctg gatgaaagct atttggaacg ttcgccattt
    60601 gaactttcag gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg
    60661 gaagtacttg tattggatga accaacggca ggacttgatc cccagggcag aaaagaaatg
    60721 atggagatgt tttggcgtct tcataaggaa cagcagatca ctattgtatt agttacgcat
    60781 ttaatggatg atgtagccaa ttttgcagat tatgtctatg tgttggaaaa gggacgaatc
    60841 gttaatagcg gggagccgca agaggtcttt caaaacattg aatggctaaa agaaaaacaa
    60901 ctaggcgtac ctactgccac cgagtttgca gaagaattaa tggctaaagg gatgaacttt
    60961 gctaccttgc ctttgacagc tgaagaatta gcagacgcta tcgtccatta tgctggagga
    61021 aatgcccatg atgaataaat tgatatttgg tcgttatatg cctggagatt ccttgatcca
    61081 tcgattggat ccccgagcta aattattagc cagtttttat tttatcggaa tcattttttt
    61141 agcaaataat tgggaaacgt accttttgtt ggcatttttc acattacttt ctgtcttctt
    61201 gtcaaaagta gatttgaaat tttttatccg aggcattcgc ccattgatct ggctaatcct
    61261 cttcactgta gctttgcagg tcttgtttac aagtggcgga gaaacttatt ggagttgggg
    61321 tgttttccat atcactgaat ttggtgtgat gaacggcctc tttattttct gtcgattcgt
    61381 gttgatcatt tttatgtcga ctctactcac gcttactaca ccgcctttgg aattatcgga
    61441 tgcgattgag tacttgcttc gtccattgaa agccgtacgg ttcccagtcc acgaaatttc
    61501 attgatgctg tcgattgctc ttcgttttgt gcctacattg atggacgaaa cagaaaagat
    61561 catgaacgct cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat
    61621 gaaagcagtc attcctttgc tgattccttt atttgtcagc agcttcaacc gtgcggaaga
    61681 tcttgcgact gctatggaag caagaggcta caaaggtggg gaaggaagaa cgaaatatcg
    61741 tgtattgcat tggcataata gagacacgat ggttatcatt gcttttgtat tattaactgt
    61801 gctactgatc tttttaagag gataaacgtt cagtgggccg ctatctattc ttttcttgct
    61861 gatctgtttg caagaaagga tgcaagcagt gtatgacaag aaatattagg gtctgtggca
    61921 gactaaggtc acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa
//
LOCUS       DAZKOK010000003        58816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000003 DAZKOK010000000
VERSION     DAZKOK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.003"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(231..971)
                     /locus_tag="ACG90W_000185"
     CDS             complement(231..971)
                     /locus_tag="ACG90W_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476582.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1101..2048)
                     /locus_tag="ACG90W_000186"
     CDS             complement(1101..2048)
                     /locus_tag="ACG90W_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0476583.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2177..2941)
                     /locus_tag="ACG90W_000187"
     CDS             complement(2177..2941)
                     /locus_tag="ACG90W_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0476584.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(2945..3334)
                     /locus_tag="ACG90W_000188"
     CDS             complement(2945..3334)
                     /locus_tag="ACG90W_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476585.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3469..4011
                     /locus_tag="ACG90W_000189"
     CDS             3469..4011
                     /locus_tag="ACG90W_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0476586.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4159..4791
                     /locus_tag="ACG90W_000190"
     CDS             4159..4791
                     /locus_tag="ACG90W_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476587.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4925..5050)
                     /locus_tag="ACG90W_000191"
     CDS             complement(4925..5050)
                     /locus_tag="ACG90W_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0476588.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5184..6077)
                     /locus_tag="ACG90W_000192"
     CDS             complement(5184..6077)
                     /locus_tag="ACG90W_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0476589.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6259..7185)
                     /locus_tag="ACG90W_000193"
     CDS             complement(6259..7185)
                     /locus_tag="ACG90W_000193"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0476590.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7270..8031)
                     /gene="pflA"
                     /locus_tag="ACG90W_000194"
     CDS             complement(7270..8031)
                     /gene="pflA"
                     /locus_tag="ACG90W_000194"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0476591.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8275..10506)
                     /gene="pflB"
                     /locus_tag="ACG90W_000195"
     CDS             complement(8275..10506)
                     /gene="pflB"
                     /locus_tag="ACG90W_000195"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0476592.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10783..13233)
                     /gene="parC"
                     /locus_tag="ACG90W_000196"
     CDS             complement(10783..13233)
                     /gene="parC"
                     /locus_tag="ACG90W_000196"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0476593.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13245..15290)
                     /gene="parE"
                     /locus_tag="ACG90W_000197"
     CDS             complement(13245..15290)
                     /gene="parE"
                     /locus_tag="ACG90W_000197"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0476594.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15456..15890)
                     /locus_tag="ACG90W_000198"
     CDS             complement(15456..15890)
                     /locus_tag="ACG90W_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0476595.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            16017..16655
                     /gene="plsY"
                     /locus_tag="ACG90W_000199"
     CDS             16017..16655
                     /gene="plsY"
                     /locus_tag="ACG90W_000199"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0476596.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16787..17662)
                     /locus_tag="ACG90W_000200"
     CDS             complement(16787..17662)
                     /locus_tag="ACG90W_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0476597.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17744..18535)
                     /gene="codY"
                     /locus_tag="ACG90W_000201"
     CDS             complement(17744..18535)
                     /gene="codY"
                     /locus_tag="ACG90W_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0476598.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18553..19953)
                     /gene="hslU"
                     /locus_tag="ACG90W_000202"
     CDS             complement(18553..19953)
                     /gene="hslU"
                     /locus_tag="ACG90W_000202"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0476599.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19967..20515)
                     /gene="hslV"
                     /locus_tag="ACG90W_000203"
     CDS             complement(19967..20515)
                     /gene="hslV"
                     /locus_tag="ACG90W_000203"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0476600.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20552..21457)
                     /gene="xerC"
                     /locus_tag="ACG90W_000204"
     CDS             complement(20552..21457)
                     /gene="xerC"
                     /locus_tag="ACG90W_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0476601.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21655..23031)
                     /gene="trmFO"
                     /locus_tag="ACG90W_000205"
     CDS             complement(21655..23031)
                     /gene="trmFO"
                     /locus_tag="ACG90W_000205"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0476602.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(23000..25078)
                     /gene="topA"
                     /locus_tag="ACG90W_000206"
     CDS             complement(23000..25078)
                     /gene="topA"
                     /locus_tag="ACG90W_000206"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0476603.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25200..26057)
                     /gene="dprA"
                     /locus_tag="ACG90W_000207"
     CDS             complement(25200..26057)
                     /gene="dprA"
                     /locus_tag="ACG90W_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0476604.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(26113..26880)
                     /locus_tag="ACG90W_000208"
     CDS             complement(26113..26880)
                     /locus_tag="ACG90W_000208"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0476605.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26873..27733)
                     /gene="ylqF"
                     /locus_tag="ACG90W_000209"
     CDS             complement(26873..27733)
                     /gene="ylqF"
                     /locus_tag="ACG90W_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0476606.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27993..28391
                     /locus_tag="ACG90W_000210"
     CDS             27993..28391
                     /locus_tag="ACG90W_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0476607.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28628..29170
                     /locus_tag="ACG90W_000211"
     CDS             28628..29170
                     /locus_tag="ACG90W_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0476608.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29253..29804)
                     /gene="lepB"
                     /locus_tag="ACG90W_000212"
     CDS             complement(29253..29804)
                     /gene="lepB"
                     /locus_tag="ACG90W_000212"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0476609.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29956..30183)
                     /locus_tag="ACG90W_000213"
     CDS             complement(29956..30183)
                     /locus_tag="ACG90W_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0476610.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30180..30698)
                     /gene="msrA"
                     /locus_tag="ACG90W_000214"
     CDS             complement(30180..30698)
                     /gene="msrA"
                     /locus_tag="ACG90W_000214"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0476611.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30718..31431)
                     /locus_tag="ACG90W_000215"
     CDS             complement(30718..31431)
                     /locus_tag="ACG90W_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0476612.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31434..32330)
                     /locus_tag="ACG90W_000216"
     CDS             complement(31434..32330)
                     /locus_tag="ACG90W_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0476613.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32488..33330)
                     /locus_tag="ACG90W_000217"
     CDS             complement(32488..33330)
                     /locus_tag="ACG90W_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0476614.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33462..34115
                     /locus_tag="ACG90W_000218"
     CDS             33462..34115
                     /locus_tag="ACG90W_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0476615.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34178..34705)
                     /locus_tag="ACG90W_000219"
     CDS             complement(34178..34705)
                     /locus_tag="ACG90W_000219"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0476616.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34725..35672)
                     /locus_tag="ACG90W_000220"
     CDS             complement(34725..35672)
                     /locus_tag="ACG90W_000220"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0476617.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35684..37567)
                     /locus_tag="ACG90W_000221"
     CDS             complement(35684..37567)
                     /locus_tag="ACG90W_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0476618.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(37730..39808)
                     /locus_tag="ACG90W_000222"
     CDS             complement(37730..39808)
                     /locus_tag="ACG90W_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0476619.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39821..40096)
                     /locus_tag="ACG90W_000223"
     CDS             complement(39821..40096)
                     /locus_tag="ACG90W_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476620.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40458..40913)
                     /locus_tag="ACG90W_000224"
     CDS             complement(40458..40913)
                     /locus_tag="ACG90W_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476621.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(41067..42275)
                     /locus_tag="ACG90W_000225"
     CDS             complement(41067..42275)
                     /locus_tag="ACG90W_000225"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0476622.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42387..42719)
                     /locus_tag="ACG90W_000226"
     CDS             complement(42387..42719)
                     /locus_tag="ACG90W_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0476623.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42867..43742
                     /locus_tag="ACG90W_000227"
     CDS             42867..43742
                     /locus_tag="ACG90W_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0476624.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43824..44405)
                     /locus_tag="ACG90W_000228"
     CDS             complement(43824..44405)
                     /locus_tag="ACG90W_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0476625.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44411..45670)
                     /locus_tag="ACG90W_000229"
     CDS             complement(44411..45670)
                     /locus_tag="ACG90W_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0476626.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45672..46718)
                     /locus_tag="ACG90W_000230"
     CDS             complement(45672..46718)
                     /locus_tag="ACG90W_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476627.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46953..47228)
                     /locus_tag="ACG90W_000231"
     CDS             complement(46953..47228)
                     /locus_tag="ACG90W_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0476628.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47471..48781)
                     /gene="der"
                     /locus_tag="ACG90W_000232"
     CDS             complement(47471..48781)
                     /gene="der"
                     /locus_tag="ACG90W_000232"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0476629.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48963..50192)
                     /gene="rpsA"
                     /locus_tag="ACG90W_000233"
     CDS             complement(48963..50192)
                     /gene="rpsA"
                     /locus_tag="ACG90W_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0476630.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50327..51007)
                     /gene="cmk"
                     /locus_tag="ACG90W_000234"
     CDS             complement(50327..51007)
                     /gene="cmk"
                     /locus_tag="ACG90W_000234"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0476631.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(51056..51691)
                     /locus_tag="ACG90W_000235"
     CDS             complement(51056..51691)
                     /locus_tag="ACG90W_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0476632.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51757..53160)
                     /locus_tag="ACG90W_000236"
     CDS             complement(51757..53160)
                     /locus_tag="ACG90W_000236"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0476633.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(53147..54178)
                     /locus_tag="ACG90W_000237"
     CDS             complement(53147..54178)
                     /locus_tag="ACG90W_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0476634.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54209..54430
                     /locus_tag="ACG90W_000238"
     CDS             54209..54430
                     /locus_tag="ACG90W_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0476635.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54595..55332)
                     /locus_tag="ACG90W_000239"
     CDS             complement(54595..55332)
                     /locus_tag="ACG90W_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0476636.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55485..56825)
                     /locus_tag="ACG90W_000240"
     CDS             complement(55485..56825)
                     /locus_tag="ACG90W_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0476637.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(57062..58585)
                     /locus_tag="ACG90W_000241"
     CDS             complement(57062..58585)
                     /locus_tag="ACG90W_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0476638.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(58635..>58816)
                     /locus_tag="ACG90W_000242"
     CDS             complement(58635..>58816)
                     /locus_tag="ACG90W_000242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476639.1"
                     /translation="SPFKRRKCLFIKKRKRYNEKEESFKNLKKEEGIGKNELFEQKFY
                     KMRVIIFVLILFLIK"
BASE COUNT        16125 a        12375 c        10013 g        20303 t
ORIGIN      
        1 attattcagt aaaatcttct ttataaaaaa ataaactatt tttgcaaacg atctgcgaaa
       61 atagtttgtt tttttatttt gccattattt tcttacttat tgggagaaaa aattcgctta
      121 aactcaaaaa aatttgagga tacgatataa actttcggat agagtataaa ataaggaaac
      181 gccgcagcgt ttcctttcca actgtagccg aatataaatt tgtatatcac ttatttaaaa
      241 tagctatttt ctcccaaaga gtttccttga tcatcgatca atccttgtaa tgagcgttct
      301 aagataccat ggcttttttt cgattttttt tcttctgact gctttgtttc ctctaacgtt
      361 tttgttcgat tgaatgttcg tacagacggc tcaccatttc ttttttcttg atacggcgtc
      421 ggttctgtat cgaacaacag ataagagtca tccgtttttt tcatcgattc gattaaatga
      481 tcctcagaaa ttttgctttc tctagggtct gggataatcg atgcgggtac atatttagga
      541 acaaaataag aacgctcccg caccttcgtg tcaacagatg cgtttcgtgt agtatgcttg
      601 gactctgtcc ttgtttcact ttgtctgaca gaacgagagg aatcaccaaa aagttttttt
      661 cgtcctgtcg atgtgatttc tgattggtaa tggccggtat aatccggtaa ttttccttta
      721 tgcttttcca aattttcctt acgcggagct gacttcaatc catttgttgc acgacgcttg
      781 cgacggattt tctcggtgtt cgtgttcgtg aaatgaaatt ctttcatctg ttcaaacggg
      841 gactgattct gttcagtcaa aagatcttct tgtccgtcaa ataatactcg cttcttgtcg
      901 ttggttaatt ttacgcctaa ctcatcatcg tacgcaggaa aacggaaatg ttttcgtctg
      961 atttctttca ttcaaaccat ccttctcaaa aatggaactt ttctcaataa tagcacagat
     1021 tcattgtttt cagtagcaat gcgctgatta ttttaagaaa atagcgatga atttcataaa
     1081 tatatatata gtaataagac ttaattcgtt gtctctctga taactagttc aggaaaaaaa
     1141 cgacgataac cttgactcgc ccctttttct tctatttttt ttaacaacat ttctccacat
     1201 acttcaccca tacgtataat atcttgcttg atcgttgtca atttaggtga actgatttgg
     1261 tctaaaaata ccccatcaaa tccaatcaca ccaaattctt cgggcaagtg tcctccagca
     1321 tttataatcc ctcttactgc tcctagtgct agccgatctg aactacatac aaaacaagtt
     1381 ttatctgtaa aatctgccca gttttctgtc atatatcttt ctacttctgt cgaatgattt
     1441 ggaaaccgca agattttact aggaagatca acttccttca tcacagataa ataaccagtt
     1501 tcccttgaaa tttcaaaagg ctcgtccact tcgataccga tataaataat cgtttgataa
     1561 tctctttcta aggcatattg cgttgctttt gctgtaccat attcattatc gctgtctaca
     1621 taatcatatc cttcagaatt ttccccaaac aagacaactg gtttttccaa acggtcgatc
     1681 caatcgaagt ctttttttct gacacctgtg atgatgtatc catcacaaga accgacgtcg
     1741 aaggcatcat ttgtaactaa ttggagagaa taatgcgctt taccgatccg tttagcgatt
     1801 cccatcagca aattcatgta atagggctca gttgtgtcga tttcttccag tataaataat
     1861 ttgatgatct gcgaccgatt cgtgacaagt gcttttgcag caatatttgg acggtaatcc
     1921 agttcagcca ttgctgagaa aactaactct ttcaattcgt cagtcacctg ttccggatga
     1981 ttgatgactc gagagaccgt catttttgaa acattggcac gttttgcaac atctgctaaa
     2041 gtgggcatgc tctcttcctt tctaacatgt tgtcattctt actatcaatt ataccaaatt
     2101 ttcctaacag agtttattat agcccaaaat aaaaaacgga aaatcctcta tcgtcatgag
     2161 tgattttccg tttcaattat ttatactttt ttatcaaatt ttttgctaaa aaagtggaag
     2221 cttcttccat tagtggtatt tgtaccatat gtcgctgtgt ttttgaggaa cgaaaaacaa
     2281 tttcttttcc gtaagactta tgccgatttt tttctacaaa tcgttccgtt tgtatataag
     2341 ggattttttc gtcagcttgt ccatgccaaa agaacagagg acgtccagct aacttttccg
     2401 gctgcaatga caaatcatag ctcttaaccc atgaaatcaa atcaaaatag tcttttggta
     2461 cgacataatt atattccgat gcgcgcctta cgatttcttt tccatattcc aaaggtgcag
     2521 gggaacccat caaacacacg cccgctacta tatgaggatg tttagccaac aaggcgcatg
     2581 ttgtcattcc ccccatcgaa accccgccaa ctgcaagctg atcatgtaga agacctcttt
     2641 tttctaaatg cttgataagt gtgtcaaatt caaacagatt tccgtagata ctatcccaaa
     2701 atgtatagga tggaatagaa gaaaccggct gtttgcgctc cccatgattc atcgcatctg
     2761 gcaggatcac acgaatgccc tgctgcgcaa tttttcttcc ttgcgtcaaa actaactcct
     2821 tacttgtctg ccatccgtga taatagatga caacgggaat acgttcctct cttaattctt
     2881 tcagaacaac ttctaacact ggaatatgat ggacagaacg tttcaatata attggattca
     2941 attgttactc acctattttc attaaatttc ctgatcgcca tagcaaccat gctccaacag
     3001 ttgtctggaa caggttcagt gtaccaaata atataaatgc aggactgaat ggctcgggaa
     3061 ataagcacag agaacaaagt cccaccactc ctaatgctag cgaaaggagt ggataaatag
     3121 aaaaattcaa aaaactggca acgctaccga aaatattacc aataaacagt atcaaaaaga
     3181 aaaaaacgct taaaattaaa ccatctgtca gcgacatctg aaaaacaatg ttctctacct
     3241 caattggctg ttgaatcgaa tagacaccta agtatatact gagaaggaga cttagcacgt
     3301 ggctggcaca aaacacacga ttgaccgttt tcattttaat cacctaacct atctttataa
     3361 ttctattata atcattttat catttttttg tatgtataaa gtttcaattt ttcttcactt
     3421 acgatttttt ttgatattgt tgttatataa atgaaaggag ttttacaaat gattgatcca
     3481 aaaatgttgg ggatgatctt cctcattaat tttgcttata tcactctgaa cacgttacgt
     3541 ttcatgttga caatgaaagg ctatcgactg attgctccac ttgtcagtat ggtcgagatc
     3601 acgatttata tcgtcggctt atcccttgtt ttagatcgtt tagataatcc actgaattta
     3661 tttgtctatg cgttaggtta cgcagtaggt atcagtgtcg gaattttgat tgaagacaaa
     3721 ttggcattag gatacatcat ggtcacaacg attttgccag caaatagcgc agaagataaa
     3781 agtttgccgg gtatactgag agaacacggt tatggtgtga cacagacttc tgcaatgggg
     3841 ctggaaggtg agcggctagt tctcgaaata ctttctccta gaaaaagcga acgtgaactg
     3901 tatcgcctga tcaaagaaat cgaagagcgt gcttttatca tttcttatga gccaaaatat
     3961 atttcaggtg gtttctggac taaaaaagtg agaaaacgac aaggaaaata actcacaaac
     4021 ttaaaaactg tctaatatct aaattaaatt ttaaatgtta gacagttttt atttattatt
     4081 ttgattaaaa aacatcgaca tatactcatc aatttgttat tatactttat atacttatca
     4141 atttaatttg gagatattat ggaaattcaa cgtagaacca ctaatttttt acatttaggc
     4201 tctttttttg caactttgac gatcttattt tttctatgtt attcatacct taagacctca
     4261 catgcgcctt ctagtgcatt aggctggttg cttttggatc attctgatat cacagaagcc
     4321 gaagaaaatc cgtttttttt gattttgtct tctctttgcg gcttactgtt ttctgtttat
     4381 ttcggtgcct atttttttca tcgacatcct ggtacgttaa acgaagcttc aagcgtattg
     4441 attgcattta aaacgttttc ttttttagca attctttttt attcgtttaa tcacaatacg
     4501 cttttcttct ttatcatgaa tggtttagtc gtgattttat ccatttttac ttattttatt
     4561 ttttcactca tttttagcca attgaaatgt ccgctcttct ttcgattagt tcctgtcacc
     4621 tgttttattt acagcttgtt ttcctttttt gtccttgctt taatgccact gaccaaccct
     4681 tcgacgatcc aagtcgcagg aaatctgttg gatatgctct tcatcgtcag tctaggtgta
     4741 tttatgtggc aaagaaaaaa caaacaaaat aaaaagcatt ttgaagcctg actattacga
     4801 cttcataaat gcttttttag ttttaaacac ttcaatctaa aagaataacc caagattgga
     4861 taaattatta atgactcgcc attcgtttct tagtgattct ggattatcaa atggacttcc
     4921 taatctaatc agctagttct tcccataaaa tgccatactt gactgaatca taatattgat
     4981 tatttaaaaa tctaactttt cttatcacta cttcctttgt cattccacat ttctcaccat
     5041 ttttttgcat tcctttgttt ccagaccatg tagtgaatct aataggtggt agataggcaa
     5101 aatactcaaa taattctttt aaccataaag gaagagaaag gctcctcctg caagagtcag
     5161 tataattttt gcttactgct ctcttaggtt ttactcacta aaactgttcc atattctttg
     5221 atataagaaa atgcatgcgg tataaatttc ttgctcagct cagaaaagat ttgtcgttta
     5281 agaaaggcta tttcctccat tgtcaatgaa ggatccaatg ttggtaattc aatctcaaaa
     5341 ttgttttttt cgacaatagg taattctaat gtgaatgatt cttcttcctt tatagtattc
     5401 gtcacaagta gagactgatg gaaaatacta ggccggcgat ttttatattt attcttacgt
     5461 atcctgtcaa cgataacttc tatttgatca aaaaagtacg attcatttac gtctccaata
     5521 agttcggcta attctttgaa tgccttcgtt acaggaagct gattcttctg tacaaaaaag
     5581 taattagcca aagtaagcgg gaagtgttct atagaggaaa gtgtgataaa acggtacttc
     5641 tcatttcctg cagttgtcct cagcaattct gtctctcggc tgtttcttac tccataaaaa
     5701 tacgcttctt tccgttcatc ttcgaaacaa tacgcccaaa taacttctcc catcgctaat
     5761 ttttgttcat caacagagag tctcttcaat tgttcagcta tggtttcagc agccgaagtg
     5821 gcttgttgca agtaatcttt aggatcaaat attggaaagt tcaaatgata tcttcgttct
     5881 tccctcttga ttaatcctgc atcgatcaat aaatcaagaa aatgggaaaa tttattttgc
     5941 gaaaattctt ttttcaattc tcttagaatc gtttcttgtt ctcgatgttc atatagataa
     6001 tcggtcaatg aagaaaaaag cggatcatcg gtcagttttt gataaaagtt ttccttattt
     6061 tttgaataat agttcatata tgttctcttt tctatccgtt ttattttact taaaaggctt
     6121 ttacgtaaaa aggaggaaga acaagagtca taattgactg ttattctcct ccatttcttc
     6181 ggcctttagt atgttctatg tttcttttta tatcaatcgc ttatccattc attttgaatg
     6241 cttcatacta ccatttgatt agctaaatac agccgtcaat tgaggtacga cttgtttttt
     6301 acgtgaaacg acacctttaa gcaaagcacg gttgttggtc aaagttgttt ggaaagcttc
     6361 ttctactttt tcttttcctt taccagcaac aagtaattct gaatcactgt ctaagatgtt
     6421 tgtgacaact aatacgaaca gatcgtagcc atttgctttc atttcatctt ccattgctgc
     6481 ttctagctct gcttgtcgat ccatcacttc attaaggtcc actgtattga cttgagcgat
     6541 acgaacgttg gaactgccca ttgggaaact ttttgcatcc aaatcaagaa gaaccgcagc
     6601 tgatttatca cttagatttg ttcctgcttt caacatttca agtccataag tgtttagatc
     6661 gacttcagcg attgctgcaa gttcttttgc agctgcttca tcttcaggcg tacatgttgg
     6721 tgatttgaac aacaatgtat ctgaaatgat agctgagagc atgattcctg cgatttcttt
     6781 aggaattgtt acttggtttt ctttatatag ttttaaaata atcgtacttg tacatccaac
     6841 tggttctgca cgatagtaca atggattagc tgtttcaaag tttgcaatac gatggtggtc
     6901 tactacagct aaaatattta cttcgctgat atctgaaata ctttgttgga attcattatg
     6961 atccacaagc atgacatctg cattttctgc tttagctgac tctactactc gtggtgcaga
     7021 tagctgaaag tggtctaatg cgtattgtgt ttcttcatta ggtgtaccta gagcaactgc
     7081 ttctgtttct ttacctaatt cattttgcaa gtgtgaaaac gcaatggctg ccccgattgc
     7141 atctgtatct ggattttggt gaccaaaaac taaaattttt gacatgtcat tcgctccttt
     7201 gtgatttctc ctatttaata atagcaaaaa aacggaaaga agcaatactt atcttctttc
     7261 cgatgcgacc tatcgcgtta aatattttgt atactcttct gtatgaagca atcgtttagc
     7321 attttcgaca cgttcttttg taggtggttc gatcccttct aatggatatt tcaaaccaag
     7381 tgtttcccac ttgtattttc ccatcgtatg ataaggaaga atctctactc tgtctacatt
     7441 tgacaaggtt tgaataaagt tgtctagtcg gatcaaatat tcatcataat cacttcgctc
     7501 aggaactagt acatggcgaa tccaaaccgg tttgttgatt tttgacaaat attgagccat
     7561 ttctaaaata ttttcattgt cccaatgcgt tagtttttta tgctcttcat tatcgatgtg
     7621 tttgatgtca aataacagta aatctgtata cttcatcaat tcttgaaaac gtgaaaagaa
     7681 aggttcttca tacgtaaaag gttttccgca tgtatccaat gtagtatgca tcttccgctc
     7741 ttttgctttt ttaaatagtt cgatcaaaaa atcgatctgc aatagaggtt ctccaccact
     7801 aacagtaata ccacctttat ctccccagta aggtctgaag cgttctgctt tatctaaaag
     7861 ttcatctgct gtgtattctt tccctccgcc aatattccaa gtatctggat tgtgacaaaa
     7921 ttggcaacgc atgcggcatc cttgcataaa cacgacaaag cgcagtccag gtccgtctac
     7981 tgaaccgaaa gtttcgatag aatgaacata gccgattgtt ttttcttcca tttaagttca
     8041 catcttttct ttgatttcat tttaagtaca agttagctgt cgaagcgaag ctttctgaac
     8101 aaaatttttc gcaattgccg cttcgttatg tgtaaaattt acagggacgg aacatacaag
     8161 tcctgcccct gtaaatcgtt tctttcaaat aagcatactg attattcgat tttgatgcga
     8221 tggctattca acgtcctttc gacgctaagt cgaaaaactt ttttcacgaa ctttttacat
     8281 tctgtcgtgt gaagttctca tcaagacatc taattgttgt tctcttgtca agtcacggaa
     8341 tttaactgca taaccagata cccggatcgt caagttcgga tatttcattg gattttccat
     8401 tgcatcacgc aataaatcat tactgaatac attgacattc aaatgataag ctccttttga
     8461 gaagtatcca tctaagacat tacgcaagtt ttcgattctt gtatcttgtt cttttccaag
     8521 acctgaagga ttgattgttt gggtatttga aattccatcc aatgcacagc cgtaattcat
     8581 tttagctgtt gagttcaggc tagctaataa cccactttgt tccgcattgt aacttggatt
     8641 tgctcctggc gcaagtggtt ttccagcttc acggccatca ggagtatttc ctgtattttt
     8701 accgtaaaca acatttgaag taatcgtcaa taatgaagtt gttggttttg agttgcggta
     8761 agttttatga cgtttgattt gtcccatgaa gtattccaag atccatgaaa ctaattcatc
     8821 tgcacggtca tcatcatttc cgtatttagg gaagtcgcca tccacttcaa aatcaactgc
     8881 tattcctttt tcattacgga ttgcttttac ttttgcatat ttgatcgctg acaagctgtc
     8941 tgccgcatga gagatcccag caatacctgt agcaaatgtg cgttgcaagt cagaatccat
     9001 caaagccagc tgagctgctt cataagaata tttatcatgc atataatgaa tgatgttcaa
     9061 cgtattgaca tatagttctg ctaaccaatc catcatattc atgaagttat gtttcacttc
     9121 ttcaaagtct agtatttctg atgtgattgg acgatattct ggtccaactt gggcgccagt
     9181 cacttcatcg actccgccat tgatggcata taatagtgtt ttagccaagt tagcacgagc
     9241 gccaaagaat tgcatgtctt ttccgacaac tgttcctgat acacagcaag caatcgcaca
     9301 atcatcagaa ccccattgtt cacgaagctg attatcattt tcgaattgaa tcgaagaact
     9361 ttcgatagcg attctgctag catatgttct gaatccttct ggaaggtgtt cagaatacaa
     9421 aacagtcaag tttggttctg gtgaaggtcc catatttgtt aatgtatgaa gcagacggaa
     9481 atcattcttt gtaactaatg aacgaccatc taatcccata cctgcaatgg ataaagtagc
     9541 ccagataggg aatcctgaga ataattggtt atattcaggt gtacgcgcga atttgaccat
     9601 acgtaatttc attactaagt ggtcgatcaa ttcttgtgct tctttttctg tgattactcc
     9661 attatccaaa tcacgttgga tatagatatc aaggaatgct gagatacgtc cgattgacat
     9721 cgcagcacca ttttgtgatt tgattgctgc taaatatccg aagtatagcc attggacagc
     9781 ttcttttgca tttgcagctg gtttagaaat atcatagcca tatttcgcag ccatttcttt
     9841 catttgcgct aatgcacgat attgttcagt gatttcttca cgtaaacgaa tcacttcttc
     9901 actcattgta tgataaccag tcatatcatg atcttttttc ttttgttcca tcaaatagtc
     9961 gattccatac aatgccacac gacgatagtc accgatgata cgtccgcgac cgtaagcgtc
    10021 tggaagccct gtaatgattt tgtttttacg agcagcacgc atttcagcag tataagcgtc
    10081 aaatacacct tgattatgtg ttttgcgcca gtcagtgaaa atatgtgtca attcatcgtt
    10141 tggtgtatag ccattgcttt ttagcgcatt gttagccata ttgattccgc cgaacggcat
    10201 gaatgcttgt ttcaaaggtt tgtctgtctg tagaccgacg attttttcca aatctttatc
    10261 caaatagcct gcatcgtgtg aagtaatagt tgatacgatt tctgtgtcca tatcatagac
    10321 accgttacgc tcgaattgga tatcatttaa cttttgcaat tcatcccata atttttctgt
    10381 tgcttctgtt gggccttcca aaaagctgtc atcaccgcga tattcagtgt agttattttg
    10441 aataaagtct ctagtgtcta cgccttcttt ccatttggta cctttaaatc cattccattg
    10501 ctccattgta tggacctcct aaatttctgt tttatgtaac agtttttacg tgattagtat
    10561 aacacagcac cctatctttg caagggtttt cttgtttttt atttaacaat ttttatgttt
    10621 tatttacata tatttaaatc aacgttgaca taccaacatt taagcattgt gaacactttc
    10681 atcttttttt gcaaacgtat aaggatttat taaaaatgaa tgaaaaagag acgccacctg
    10741 tattaataca gatggcgtct cttcattaaa atactacaac agttacgact cgatgaaaat
    10801 ctcaggagca tcgataactt cgatgacttc tccacctttt ttctcatcca tcgcaaatga
    10861 tccattcgat gttcgatcac ctatagggaa atcagttggg tcaatcgtta actgctgccc
    10921 tttttgattc atcaaggtta tttcttttga tcttccttcc gacatataga taatacggtg
    10981 cggatttttt ttcagctcac gcatgaccgt cactccccgc ttcgcacgtg aagtttggct
    11041 gatttcttga gcaagcatgc gtttaacagc tcctcgctga gtaacgatta cgatcggcgt
    11101 atcaccttct gctaacacaa gcagcccgtt gataatccta tctccatctt tcaaattcat
    11161 agacttcact ccagctgcct ttgctccaac aacgggaaca tctgttaaag gatagcgcaa
    11221 acccattcct tgatgcgtta ctaagaaaac atctaagcct ggctgatctt ctttcagata
    11281 cacagcaatc agttcatctt cttccgcctt tagcttcata cagctgagcg gacggctctt
    11341 ataggtgcgc catggttcga actctgtcat tcgtgtttgc ttgatcaatc cattcttgct
    11401 gatgaataca aatgttttct ctgcttgaat tttttgatat gggaaaacag ctaaaatcga
    11461 ttcgtctaca gacagattgg agattgtttg agaaatgtgc tcaccagcat ctttccattt
    11521 caaatcgggt agttcatgga cagggcggta gatcacattt gctttatttg tgatcagaag
    11581 aacatggtcc aatgtactca attcacctga gtatagcaag tagtcgcctt ctcgcatccc
    11641 gatttcttct ggtttggaag cactataaga acgaaggctt gttcgcttca catagccttc
    11701 tcgcgtcaca ctgacaatta cgtcttcttg tgccactaag acttgcgtat caattttgat
    11761 ttcttcgatt tcgtcttcga ttgtcgttag acgagcggaa gaataatttt ttttcacttc
    11821 tcgcaattct tttttcataa cagagaaaag ctctttgtca tttgaaagga ttttattcaa
    11881 ctctgtgatt tgcgcaatca agctttcgga ttccttttga agctctgtga tatccgtgtt
    11941 cgtcaaacgg tataattgca atgtgacaat tgcttctgct tgttcttctg taaattgaaa
    12001 gacgtcgact aaattctttt tggcgtcttt tttgtcttta ctttcacgaa tcgttgcgat
    12061 tacttcatcc agaatcgata aagctttcat caatccttct acgatatgct gtcttttttg
    12121 agctttggct aaatcgaatt gactacgttt tgttatgact tgctttcgat gctcgatata
    12181 acttcttaaa atgtctttca gtccaacttg atgcggagtc atatggtcga tagcgaccat
    12241 attgaaattg taattgatct gtagctcagt gtttttgaac aaataattca aaattccttg
    12301 agcattggcg tcttttttca attcgacgac aatttgcaga cctgttctat cactttcatc
    12361 tcgtacttca gctattccat cgattttctt attcaaacgg atctcatcca tttttttcac
    12421 caaggtcgct ttatttactt catatgggat ttctgtgatc acgatttgct gtttattacc
    12481 cttcattgcc tctatctttg ttttcgaacg aagaataact tttcctcgtc ctgtttcata
    12541 agcttttttg atttcatctt ttccttgcaa gatccctcct gttggaaagt caggtccagg
    12601 aatatattcc atcaattttt caaggctcgc ttgtggatga tcgatcatat agattgtgcc
    12661 atcgatcact tcagctaagt tatgagtcgg gatttctgtt gcatatccag cagatatacc
    12721 tgtcgatcca ttgacgagaa ggtttgggta tcgagctgga aggactgttg gttctttttc
    12781 tgtatcatca aaattccaca cgagatcgac tgtttctttc tcgatatctg ctagcatctc
    12841 tccacttaac ttggataatc tggcttcagt ataacgcatc gcagcaggtg gatcaccgtc
    12901 catactcccg ttattcccgt gcatttcgat cagtacttcc cgcaacttcc agtcttggct
    12961 catgcggacc atcgcttcat aaatactgat gtcaccatga ggatgataat tccccatgat
    13021 atttccgaca gattttgctg atttacggaa tcctttgtca tacgtattgc catctttgtt
    13081 catggcaaat aatatccggc gttgcactgg cttcaatcca tcacggatat caggcagtgc
    13141 acgttcttgg ataatatatt tggaataacg tccgaagcgg tcacccatca cttcttctaa
    13201 agttaattct tgtacttctt gtcgattttc catcaggtga cctcctattc tacttcaaat
    13261 aactgattgt tttcattttt atctgctcgt tcttcatcta ataaatcatt agaaactgaa
    13321 ggagagattt cagtatcctc ttttttatct aagatactgc catcttcttc gagcgtaaat
    13381 tgcacgtgat tttcgatcca ttttcttcga ggttctactt tgtcacccat cagtgtggtt
    13441 actcgacgtt cagcttgcgc agcatcatcg atacggacgc ggatcaacgt acgggaagtc
    13501 ggatccatcg ttgtttccca caattgttcc gcattcatct ctccaagtcc tttatatcgc
    13561 tgcagcatat atcctttgcc gactttttta atcattgctg ccaattcgtc atctgtccac
    13621 gcatattcaa tgacttgctt ctttccttgt cctttggata ctttatataa aggcggtaac
    13681 gcgatatata ctttcccagc ttcaatcagc ggtttcatat aacgataaaa gaaagtaagc
    13741 agtaatactt gaatatgggc gccgtctgta tccgcatcgg tcatgatgat tactttgtcg
    13801 taattacagt cttcgataga aaactcagga ccgactcctg caccaatcgt atagatcatc
    13861 gtattgattt cttcattttt caagatatct tgcattttag ctttttcggt attgatgact
    13921 tttcctcgta aaggtagaat tgcttggaac tttctgtctc ttccttgttt agcagatccg
    13981 ccagcagaat ccccttcgac taaatacaat tcatttttct tagggtttcg ggattgtgcg
    14041 ggtgtcaatt ttcctgaaag aagagattcg ccttttttac gcttcttccc atttcggctt
    14101 tcttctctgg ctttacgtgc tgcttctctc gcttctctgg ctttgattgc tttacggacc
    14161 aacatctggc tcatttcgct attttcctgc aaataaaagc ccatttgttc actgatgacg
    14221 ttatctacta cggtacgagc aactggcgta ccaagttttt cttttgtttg cccttcaaat
    14281 tgcagaagat tttcaggtac acggatagaa agtacagcag ctaacccttc tctgaaatca
    14341 ctaccttcca gattcttgtc ccgttctttc agcaaaccaa cttttctcgc atattcatta
    14401 taagatttcg tcatcgccgc tttcatacca gcttcatggg ttcccccatc ttttgtccga
    14461 acgttattga caaaagataa aacattttct gaataaccgt cattgtattg ataagcgacc
    14521 tctacttcga taccttcttt ttctcctgaa aaataaacaa ctggtgtcaa tgtatctttt
    14581 tcttcattca gataatcaac aaattctttg attccttctt cgtaatgaaa aatttccttg
    14641 actggttctt ctccacgaag atcagaaaga gagattttaa cgccttttag aagaaaggca
    14701 gattctctta accgttcggc taaaatttca tatgaaaatt tcgttgtact gaatattgta
    14761 tcatctggta aaaaatggac agtcgtcccg tttgcttttt ttgtttttcc tatctttttc
    14821 aaagtaccgt caggtttacc gccgttttta aatttctgct gatactctac gccatctcta
    14881 acgatcgtta caattagcca tttcgataag gcatttacca cactggctcc tacaccgtgc
    14941 agtccaccag aagttttgta ccctccttga ccaaactttc cgccagcatg cagcacagta
    15001 aaaatcactt caactgttgg tattccggaa gcatgcattc ctacaggcat cccacgtccg
    15061 ctatctgcaa cagttatact attgtcttca tggatggtca catcaatctc actgccataa
    15121 cctgataatg cttcgtcaac tgcattatcg acgatttcat agactaaatg atgcaagcct
    15181 cggctgtcag tggatccaat gtacatccct ggtctttttc taactgcctc taatccttcc
    15241 aatacttgga tggacgagtc attgtattcg ttgtttactt tttttgccaa ggcaaacgct
    15301 ccttcaataa tactttgcgg caaaaccgca ccacgattat catactagaa aaggtcgtga
    15361 aaaaaaagag ggtagacgag aaatcaattt taattatctc ctaaccgctt tttcgacatt
    15421 cccctctatc gatcatctct tctttgtggt catttttatt tttggttttc tttcaactta
    15481 ccaagttcaa tcttaataca acgatccatt acgatatctg tacgtcctgc ttttcttagc
    15541 atctcagctg cttctttgtt ttcaatccct aattgtgccc agaacacttt agcatcagtt
    15601 ttcaaaaaat cacctgctat ttcaggtaaa aattcactcc gtctaaatac atctacgata
    15661 tcgattggat catcgatttc tgtcaaattc gcatagactt tctcgcctag aatttcttca
    15721 ccagccaata atggatttac tggtatgatt ttatatccat tgtcttgcaa tacctgtgca
    15781 accatgtaac ttgttcgttc tggtttattg ctcaagccta cgacggcaat attttttgct
    15841 tcttccaaat agttgaaaat cgtttcttgt gttggatttt ttacagacat ttctgccacc
    15901 ccttgctttt ctttttattc catctattat acttgtatct ttcatttttt caattgttcc
    15961 tgttacctat tcatgctaaa ataaaggata gttttcaact tgaaagaggg taaaaaatga
    16021 aattgatctt attacttatt ttagcatatc ttttaggttc tattccttcc ggtgtctgga
    16081 ttggcaaatt gtttttcaaa aaagatatcc gacaacacgg gagcggtaat actggaacga
    16141 ccaatacatt tcgtgtatta ggcaaaacag ccgggattgt tgtcctgctt atggatattt
    16201 taaaaggtac attggctact tgtttgccga tgatcttcca cctttctggg attaaccctc
    16261 tttggtttgg ggtatgcgcc attcttggcc atacatttcc gatttttgct gggttcaaag
    16321 gaggaaaagc tgttgcgaca agtgcaggaa tgcttttagg atttaatcct gtattttttg
    16381 tttattcttg tatcatcttt atcgttagtt tattttgtac aagtatggtg agtttgagca
    16441 gtatgatcag tgcagtattg atcactttat ctacgatcgt ccttccttat gtagctcctg
    16501 tgattttggc taagcccaat tggttactga cgatcattgc ctttttagtt tcttccttta
    16561 tcttttatcg gcataaagac aatatcaagc gtatcagaaa ccatactgaa agtcgaatcc
    16621 cgtttggttt gaatagcagc aagaaaaaat cctgatactt tttcattcca aagagataaa
    16681 acgggagtgt gacaaatcga tgtcacactc ccttattact tacaggatag cttttttaca
    16741 caccttggta aacggtgttc cctgctaact tctcggtcac aacctcttac ttcactgtga
    16801 tggaatattt tgtcttgaag atttcatggg cacctagttg attgatccct actttttcca
    16861 tcaagtttcc actactgttc accgtgtcag cgattccgca ccacggttcg atacaaacga
    16921 aaggcgcttc ttttggataa ggagaccaga tccctacata aggcatatct ttatagctta
    16981 aagttacgct atgggaagat ttttcacttc gaatggcgta cgcatttaga ccccttgttt
    17041 caaaaaccaa ggcatcttgc tcaaaaagtt cacgattcaa agccagattc gtatttgtct
    17101 gacctaacgt cttttgatcc atatcgatga atgggccttt caatggtagg cgtaatcttg
    17161 atttcaatgg agaaaatgcc agatagaaat cttcaaatga caatccttct tcaagaggta
    17221 cattgaaagc aggatgtcca ccgatagaaa agaacatttc tttttcaccg gtatttgcca
    17281 cttgataatg aaccgtgatg ccgtctcctc ctaattcata gctgatcact aattcaaaat
    17341 caaatggata cttttctaat gtctccggcg tactccttaa gatgaaacta gccatttctt
    17401 gtccatgttc aatgacttca aaatccatat cccgagcaaa tccgtgttgc cccatttgat
    17461 atgttttccc ttgataaaca tactgatcat cttttaaacg tccaacaaaa gggaataaaa
    17521 ccggtgcatg tctgccccaa tacgctggat caccttgcca gatatactct aaaccatgtt
    17581 cttttgattt cagactgatc aattccgcac cgtgttcagc gatttcggcg atcagctggt
    17641 cattttgaat aataacagtc attgccattc ctccaacttc gacttacttt ttaaaatggg
    17701 aaggctggaa caaacatttc tatctgcccc agtcccctta cttctaataa ctgtgttttg
    17761 ctaactcgtc tttaaaacgg gaattcaata cttttaaata agttcccttc atgcctaacg
    17821 aacgagattc aatgattcca gctgattcca attttcgtaa agcattgacg atcacagaac
    17881 gagtgatgcc gatactatcg gcaatattag aagcagtcag acgaccttca tctccatcca
    17941 atgcttcaaa gatagcttgt acagccttca attcgctata agataatgtg ttgattgcca
    18001 tttggacagc cgttgcactc cgtacatctg cttcgatgct tcgagacttc tgataaagga
    18061 tctgcatacc gacaaccgta gctccatatt ctgctaagac tagatcgtca tctgcaaacg
    18121 attgttccat acgagataag ataatcgtac ccaaacgttc gccagctcca aagatcggaa
    18181 cgacagtcgt cagaccaaat ggatatttct ctcttgactc taccggaaat gcagtaatat
    18241 cactagtaat tgggatatta gcttcagttt tcatcaactg tgccactgta tcagtataag
    18301 attcaggaaa ttgtttctca acaaacattg ttttgattcg ttcattgttc acatcgtgac
    18361 gttcattata acctagtaat actccgtcac ttcctatgat ataggtgttg ctgtcaagaa
    18421 taccacctag tgtgacagcc attttatcgt aaggcaaatc tgcttgcatg tcaaaagtat
    18481 tcttttgctg caaaagtgtg tttatcttgc gggttttcgt tagcaagtct gtcattgtcg
    18541 tcctccgatt ttttataaaa tatagcgact taaatcttca tttgccgcaa ttgagccaag
    18601 tttgtcatcc acatatgctt ctgtgatcgt tatttctccc atttgcatat ctggtgcttc
    18661 aaataataga tcttctagta gtttttccaa aatggtatgc agtctgcgtg cgccgatatt
    18721 atctgtgtca cggttgacat cgaaagcgat ttgagcgatc cgttcaattg cttcttttgt
    18781 aaacgtaact gacacatttt ctgtaccgat caacgcgacg tattgtttta ctaaagcatt
    18841 atttggttcg gtcagaatct tcacaaaatc ttcagctgtc aaatcatcta attctacccg
    18901 tatcgggaag cgtccttgaa gttctggaat tagatcactt ggtttagaca aatggaaagc
    18961 tcctgaagca atgaatagga cgtgatctgt ttggatgatc ccgtattttg tgtttacttg
    19021 tgaaccttcg acaattggca atatgtctct ttggacgcct tctctggata cttctccaga
    19081 ttgctgtgat ttagaagtga tcttatcgat ttcatcgata aaaataattc cactttcttc
    19141 cgctaatttg attgcctcac tatggatatc agcatccttg actaattttg ctgattcttc
    19201 cttgatcaat aactctaaag cttctttgac tgttaccgtc cgttccactt ttttcttcgg
    19261 tgtcaaagca cctagagttt catttaggtc gatccccatt tgctccaatc cattgttcat
    19321 cattggtgca ggtttctttt ggtcttcaac ttcaatggtt acttcacgac tatccagtaa
    19381 acccttttgt agttgttcca agactgtacg gcgattgatt cgaatatctt ctgtcacttc
    19441 ttcttgcggt tcagcggtat tttgctgact agcattaaaa agattcatca tttgttcaaa
    19501 ttgattagtg gattgtttct gttctttttt gataccaggt accaagattt ttaccaaacg
    19561 tttttctgca ttttttttag cacttgtgta tacacggctg tactgctctt tttctacgat
    19621 ttggatcgca ttttctacta aatcccgaac cattgattct acatctcttc cgacataacc
    19681 gacttcagta aattttgttg cttcgacttt gacaaatggt gcattgacga tttttgccaa
    19741 acgtcgtgcg atttctgttt taccgacccc tgtaggtccg atcatcagca tattttttgg
    19801 tgtaatatct tgttgcattt tttcatctaa ctgcaaacgg cgatagcggt tacgcaaagc
    19861 aacggcaaca gatttttttg ctgcgtgctg tcctacgata tattcatcta attcacttac
    19921 gatttgtctt ggtgtttgtt ttaattcata catatctact cacctcttac agttcttcta
    19981 cgataatatt atgattagta aagatacaga tatctgcggc aatatttaac gcattttctg
    20041 caatttcttt cgcactcatg tcgttttgag catagttttt cattgctcgg gctgcagcca
    20101 acgcaaaatt ccctcctgat ccgatagcta agataccatc atctggtgtg ataacttctc
    20161 ctgtacctga aacaagaagc atttctttat cattcattac gatcagcatc gcttcaagtt
    20221 tttgcataga ttgctgtgtc cgccattctt gagctaattc gacagctgcg cgttgtagat
    20281 tcccgttata ttcgtttagt ttgccttcaa atttttcttc taaagtaaac gcatctgcaa
    20341 cacttccggc gaaacctaca acaacttctc cattgtagat acgtcgtact ttctttgcag
    20401 tgcctttcat gacaacagat tcacccattg ttacttggcc atctccagcc atagcaaatt
    20461 tcccgtcttt ttcgacggca cagatagtgg ttgaatgaaa tggtgattcg accatgtttg
    20521 tttcctccta aatttgttct tttccttatt tttacgctct tggatggaaa gaacgatagt
    20581 ttttttgtaa actttctttt gttacatggg catagatctg ggtagtcgat aaattggcat
    20641 gtcctagcaa ttcttggact gtccgcaaat ctgccccatt atttaataaa tgagtagcaa
    20701 atgtatggcg cagcatgtga ggatggatat cactatccaa actgcttttt ttgatcattt
    20761 gattcaagac atattcaatt ccagtaggcg tgattggttt tccatgatga ttcacaaaaa
    20821 cgatatcatg ctcttcgtga tattttgtca tcaatacctg gcggccttcc ctgaaatatt
    20881 cttgcagagc atcttgagca aaagaaccta gaggagcata tctctctttg ttccctttac
    20941 catggatcaa catgacctct gaatcaaaat caatgtctga taccgacaaa ctgctacact
    21001 cacttaaacg gatgcctgac ccgtaaagga cttccagtaa agcgcggttc ctcaaatcca
    21061 aaggactatc tcctgctacg ctgtcaaaaa gtgcttgcat ttcattttca taaaaaaaac
    21121 gtggcagtct caaatttttc tttttgagat gaacgtaaga aaatggattt tcttttatca
    21181 cttcttcttt gagaagatac tgataaaacg aacgtaggct ggctatcttg cgactgatag
    21241 aatttctgct atagctagga cgatgatcag ctaatgcact caaatagaca cgaaccgtaa
    21301 aatattccgc cttcagcaga tcagcgtctc cagattcctc caaaaaatgt ttgaaatgga
    21361 gaatgtcttc ttcatatgct tctttcgttt tttctgaata tcctctttcg acaatcaaat
    21421 aacgcaaaaa tttttgttcc cacgtggctt ctgtcatttt ctcaccttcc ttttgtccca
    21481 gagaataatt tagcacagcg tttataaaaa gacaaataaa ttgtcagaat ttaaccaata
    21541 tttctaagtt tgttcacaat tggcgcaatt cctcacattt tgtttgagaa attcttaaga
    21601 aaaacgcaag aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc
    21661 atttgggatt gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg
    21721 ttgtgcaatt gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa
    21781 cagaccatag ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta
    21841 agccatactt ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa
    21901 gcgtgcggcg ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac
    21961 accagtcatc tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt
    22021 cagcaattct ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc
    22081 agcattttct aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg
    22141 agtctgaaag cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat
    22201 aacagcataa ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc
    22261 gaaaagcatc gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa
    22321 atatttttct ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg
    22381 gaaagctttg aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc
    22441 atagcgggac ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc
    22501 agctgcatca tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa
    22561 tggatgagaa gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat
    22621 ttcttcgttt atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga
    22681 aaaagaatcg cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga
    22741 agtgattact accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc
    22801 caaactgttt cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg
    22861 cgcctcagtc gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc
    22921 gatctgccaa gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa
    22981 tgaaagaacc tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga
    23041 ctacttgttt accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat
    23101 ttctgcccac tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat
    23161 agaatagtct gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc
    23221 aagtcacacc gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc
    23281 aagcataaaa cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac
    23341 aatcgaatcc tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg
    23401 tcagttcttt ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc
    23461 cttcttcaat tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt
    23521 gagggaagaa ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc
    23581 ttttttgtgt caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag
    23641 tggaaggtcg ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac
    23701 gtgctggtgg ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc
    23761 cttcttcaag ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca
    23821 catacacttg catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac
    23881 cattttgttc taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca
    23941 caaaacgtga ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga
    24001 gttcatctgg tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc
    24061 cttgagcatt tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt
    24121 caataaatgc tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca
    24181 tgtatgtaat caagccgact gttccttgtt tacccaaagt gattccttca tataattgct
    24241 gtgctaccat catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca
    24301 ggctacttgt agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt
    24361 taacttcgaa gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg
    24421 gtaatttctt tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt
    24481 ttttaaaatt accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt
    24541 cacggtcaat aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct
    24601 tgaccttacg ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac
    24661 gagcttgctg tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt
    24721 tgactgcgtc ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc
    24781 caagtaggta cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca
    24841 agtaaacttt ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc
    24901 cgcgtataga aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt
    24961 tcggtaaatc tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt
    25021 tctcaattgt cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta
    25081 ctgaatggac tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg
    25141 tagctaatca tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt
    25201 tattctttaa attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg
    25261 atcaaatgat gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact
    25321 gttcttccaa gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct
    25381 tcaaagacac atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa
    25441 tgctttttcg ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc
    25501 acctcttgca agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact
    25561 gcgattgtcc gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc
    25621 gcgcagccac tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact
    25681 gtttttttgg ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa
    25741 agtaaagata gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc
    25801 tgagggggat atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc
    25861 cagttagcgt gaatttcctg ccaacttttg cgaaacagct cccggtgttt ggttatttta
    25921 cctatcttaa tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta
    25981 gcaagtgccc attttccttg actactgatt ccttttgtat aagcaagttt caataaattt
    26041 tcttctattt gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa
    26101 agcggctgtt ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg
    26161 cgtaattcct tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg
    26221 cgagaaacca tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac
    26281 tttagcaatg atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt
    26341 ttcctgctga ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat
    26401 ggacaatttt tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc
    26461 atcgattgtc tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg
    26521 ttgataaaga agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat
    26581 gatctcattt ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct
    26641 tcctacttca tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa
    26701 gcaaaacatt tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa
    26761 agaagccagt gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg
    26821 atcatctatt ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat
    26881 cacttttttg ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac
    26941 gaatatcatg cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg
    27001 ctgtaatatc catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat
    27061 aacgatcgct gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta
    27121 aatcatctaa atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga
    27181 tttcttcatc ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat
    27241 tacctaaacg aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa
    27301 ttttcttgcc tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga
    27361 ccattgcccg aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt
    27421 tcaatgcttc tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga
    27481 tagctaatgc aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg
    27541 gatctgccag atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat
    27601 ctagcattgg attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt
    27661 ccacatattt cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc
    27721 attggatggt cattgttctc tccattcctt tcataccgga atattccccg gctttacttg
    27781 tttttttctt tttcaatata aattcacttt tttattataa agaaattcca tctattagtt
    27841 aagtgaaagt gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt
    27901 ttattttggt ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga
    27961 tagacgaaca aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc
    28021 ctgttgctga tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg
    28081 aagaattttc tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc
    28141 ttctctctca tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa
    28201 aaacaagtgg tgctttgatt tcgtttagtg ttgaaaatgc ggatgaagta aaaaaatttg
    28261 ctcaaattgc aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag
    28321 aagaaatgtt ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga
    28381 tgaagatgta agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg
    28441 atacgataag tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt
    28501 ataaaagcag tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca
    28561 atgtgtcatg acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga
    28621 gaatattttg aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag
    28681 attgttgtcg acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat
    28741 tggtcctgct tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct
    28801 atatggaccg atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat
    28861 atttggttca ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat
    28921 taatggctta tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt
    28981 attgattatg atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg
    29041 tgcagatttc aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat
    29101 tcaaataatg gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg
    29161 agctgtttga ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg
    29221 cagtctcaac agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt
    29281 ggataataga caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa
    29341 cgactatctt ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc
    29401 ttggattgtc ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga
    29461 tctgatttca ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat
    29521 tgatcatttt ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc
    29581 gtgccatctg ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc
    29641 atcacaacca tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc
    29701 gggatgaata aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg
    29761 aatacaagcc agaagcgatt aatatagcgt tgtttttttg tcaatctcta tcaatccatc
    29821 cctctttttt tcattctttc tttattataa gaaaaatatc aaaaaaatgc gactagatag
    29881 ctgaaataaa aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa
    29941 gtacaaaact atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc
    30001 aaagatatcc atggacggca aatagtcgac attcagttcc agataattag aaatttcttc
    30061 gtagctagag ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga
    30121 taaagcatca tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt
    30181 cacccttcgt tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta
    30241 aaattttcag gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt
    30301 tgggctggtt cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt
    30361 ttcttacttt tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga
    30421 taattgtcac cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag
    30481 taaagttcta ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct
    30541 tctgtatgtc cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct
    30601 ccagtatatc ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata
    30661 caccagaagc agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta
    30721 ctcatccgat gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt
    30781 gttggtattt gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg
    30841 aatgtcatca tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg
    30901 gaattgatcc aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg
    30961 taatttatag tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc
    31021 gatagaaaga cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa
    31081 atataaatag aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc
    31141 attttccaaa taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa
    31201 gctgatgatt tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg
    31261 tctttccgta agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct
    31321 gcctaaaaat acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca
    31381 gtaatttgtt ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga
    31441 tcatcctcct tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt
    31501 gccatcaatt gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta
    31561 tttttgatgc catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg
    31621 acacctactt cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag
    31681 ttcggcattt tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc
    31741 atgtcagtga tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata
    31801 ggtgcggttt tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag
    31861 tttttcagtg gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact
    31921 ttcatcaaat cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt
    31981 cgaatttctt cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca
    32041 ccatagtttt ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca
    32101 aggggaacga atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg
    32161 atagctatgt agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt
    32221 ttcattagtg ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca
    32281 attaataatg ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct
    32341 cttgtattcg attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa
    32401 aaaagaaaac aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt
    32461 caggctgagg gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc
    32521 tttaccagta tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg
    32581 gaataattct tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga
    32641 aataccgatt tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca
    32701 tttatcgaac gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa
    32761 attcatcacc acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct
    32821 tccacctttt accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg
    32881 tacttttttg acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc
    32941 tgctcgaatt acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac
    33001 tttcgttttt gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt
    33061 tgtcatatgg atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat
    33121 gaattctcca attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc
    33181 cataaatctt tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat
    33241 ggatagcggc atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga
    33301 agaatctgtt acgattttta catttgtcat tattttctct ctctctttct ttaacaagat
    33361 gccttattca tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata
    33421 atgatttttc attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta
    33481 atcgaacgta tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg
    33541 gattgagtat cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc
    33601 tccatattgt ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta
    33661 ctttgtttca ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata
    33721 gttcgatttt tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag
    33781 gatggctagg ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat
    33841 ataaagcgtt gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata
    33901 tttttatggg gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga
    33961 ctggtgttat tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata
    34021 gcttgaattc tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag
    34081 gttttatgta cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg
    34141 acagctctaa ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc
    34201 cgctttttac aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt
    34261 tttcatccat ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg
    34321 gaaaataagc atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa
    34381 atggcagaaa ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt
    34441 ctttttccag agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat
    34501 aggaagaatc tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc
    34561 cttcgaatgt ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt
    34621 tcagatcatt tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat
    34681 cctgtgccca aattgcagct aacattttct catcctttct gattctaaac agcgatagga
    34741 gctttgatcg ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca
    34801 aatactgact gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc
    34861 aattgttctt tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga
    34921 acaaattcgc caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa
    34981 cttgcaatgt tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag
    35041 cttagccgtc cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg
    35101 ctaggaacat cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt
    35161 ttgattgctt cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag
    35221 tgacgccatt gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca
    35281 tcggttagga tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca
    35341 ggatctgtca aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt
    35401 ttgatgtagc gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag
    35461 cggatattcg tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt
    35521 actcgtttcg ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg
    35581 aataagctta atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat
    35641 tttcttccta agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt
    35701 cactcaagta ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt
    35761 cgttttgcaa ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga
    35821 gcaatgagat tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt
    35881 gttccatgta agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg
    35941 ctttttcagc aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt
    36001 aataaagttc ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca
    36061 agaaataacg gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt
    36121 ccaaaatcgt caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat
    36181 taggttgctg aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt
    36241 tgcctatcag tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta
    36301 ctccgatttg actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa
    36361 tcatccgttt atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg
    36421 tttcaccgat agacattgta ccgctatcca ggggaattct gccagctaga atattaagca
    36481 atgtcgattt tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca
    36541 ataaatctaa atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt
    36601 ccagtacttt tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt
    36661 ttacttggta caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg
    36721 tgccacgtgc ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc
    36781 gcttttcttc ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt
    36841 cataatttcc tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta
    36901 ctctatctag aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca
    36961 aatagttttc caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta
    37021 atagtaaatc gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac
    37081 ctgataaagt actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct
    37141 ttgcgttggt atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat
    37201 aacgtttttg cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat
    37261 atgctcgaac agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta
    37321 attcatcgga aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa
    37381 acactgtttg ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg
    37441 ttttacccgt cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag
    37501 aaatctggtc aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt
    37561 ctttcaacat gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga
    37621 aaattttttt ggtatttgtt ctttttgtgt tctcaaaaaa agaacaggac cgattcgatc
    37681 ctgttctgat atgttttgtt ttttataaaa cttgaaaatt tttacctttt tattttattg
    37741 gactcgtttt ttttgacttt gctcgaaacg caaatcggaa agaaaagtta tcagatggtt
    37801 tgttcatcgt ataagattct aacaaattga tattcctgcg atagctgttc gcagaaaacg
    37861 tcgtgatttt ttcagcaact tcgtattcat caatgatttt tctttcaaga taataacctc
    37921 ttagcatttt tttcgtaaac agactatctt gctggatcat ccacgaagaa atcaaattcc
    37981 ccgagttcat ttttattcct tcaagtttgc gttgctggat aaaaaattga accatcgttt
    38041 catcataaat atcagtcaag ttatctaaac tttcaataat caactgagta tttttttgat
    38101 atgtctctct gatttcttgg atatcttcat cactaataaa tgtctgacga tcagcatatc
    38161 ttctttgcca aaacattttc ggatggagaa cgattcgcat taacctacgg acaacaatga
    38221 accaaaagcc gataaaagac aaaatctggc ttgtgataga atgttcgaat cgagaaagaa
    38281 aacgggagta aaaacgataa ccattagaag aaagcttgcc cgaacgataa ctttcatcca
    38341 atccatcccg ttcgatcgac aaaatcagtg cttgtatttc ttgtacttct tgcttttcat
    38401 catcggttaa atcttccaag tacagatcac gtatccgttc ttgataattt tctatcactg
    38461 cttcagtggc taaaaattct tttgtattac gatcaatttc ctttatttct tttcgcaaaa
    38521 cttcaacgac ctcttctaaa atcatcaaag cattcaaatc cgtgcttttc gcttcctctg
    38581 tttccgtcag cataggcaag acaatgatcc ctatgaccaa agtcactaag atgacacccg
    38641 ccgtcagaaa caaaagcaaa gaccgctggt aaaacatcat attattgatt gaaaatggca
    38701 aaataaaaat cgtagccaaa ctgactgttc cctttacacc gccaaatgtt aagatcaaga
    38761 tctcgttcaa ctgttttttg aactttttac tgccatcttt gaatacataa aaaagactga
    38821 tagaaatgaa tctgatcaca aaaagcatga cagagattaa tacgataatc gctaataaaa
    38881 gaccgtttgg atataaaccg tctccccata ctggcgagaa cacttgcgtc aattcgattc
    38941 ctaaaaatat aaatactaat gcattcagtg taaaagcaat agttgtccat gtactgtggg
    39001 acaagctcga caattctgca tcaaacacag agattttcct aaaaccagaa gcttggagaa
    39061 tacctgctgc cactgcagca atgattccag atacatcaaa tgcctctgcc aatacatagg
    39121 ctagaaaagg aagcaaaagt tcgatcagca gataccctgt gacatcttgt gctgatgctt
    39181 tttcgatcaa atgaattatt ttttgtttga accagacaag caaaaagccg atcaaagctc
    39241 ccccaatact agaaactact agactcatcc ccgcgtttac tgctgaaaaa gatccggtga
    39301 tcagcgcacc aagagcaaac tggaaggcag taacaccaga tgcatcattc aaaagacctt
    39361 ctccttccaa aatatgcatc gcctttttag ggatattcac tcgtccagaa agagaactga
    39421 ctgctaccgc atctgtcgga cctaacgctg ctccaaaagc aaagcaggca gcaagtggga
    39481 cacttggcaa caggaatgat aacgtcgcac ctaccccgac aagggtcaaa atgactcctc
    39541 caaatgccaa gaacaaaatt gtcccgaaat ttttgagaat cgaagaaata tctgcttttt
    39601 ctccttctct aaataacaat ggtgcaataa tcataactaa gaacaattca ggttcaaaat
    39661 cgattgattg tccccactct gtcagaccta ataccactcc aaggaaaatt tggatcagtg
    39721 gagctggaat cattggcaaa atgcggctag ctatgttcga taaggtgatc gttactgcaa
    39781 atacaataac taactcagca aattccatcg tccatcccct ttatttttta ttttgtgtaa
    39841 gcatttttaa aaattgttct tttttcttta aaattttggt ttcgactact tcaacctgtg
    39901 tttggttccc ctctaattca cactgtacac gttttgactc caaacgaagc agatcatgtt
    39961 tcagacgttc caactgacga tacatcaata tatttctttt acgcaactta caaagtattt
    40021 gacggcggtc atccatcgaa agggcgccat agcgatcatg caacagtttt ttttgttgtt
    40081 caaaactctc tctcattttc cagatctcct atacacaaac ttccatcatt atagcaaagt
    40141 tctttttgga tgaaaaatat ttcattgttt catcgtttat tgctagttag aaatttccat
    40201 aaatttttca acgcctcatt ctattccaaa tagtatcaga tatcaagtaa aacttaagaa
    40261 agataaaagt ttctgacaaa aggatgttta ctttcccatt cctgcctttc ttgttctttt
    40321 atcacaacaa aaaaatgggg ctgcgacaaa aatcttgtca cagtccctta ttctgaataa
    40381 acggtgtgga agagacagcc tatcagtagt aataagacac atctctttca cagcctcatt
    40441 tttgcgtaaa aatcgattta ttcgaaaaat tccccgtata catcttctac acgttgatct
    40501 ggattgatca cgatatacaa gttacctgtt ttttcgctga tttttgttga ttgatattga
    40561 ttgtctaatc cttctgttcc tttatctaga tatgggaagt acacaagatc aggtgtgcca
    40621 tttcggtaaa ggatttccat tcgctcgata tcttcgtatt tcaatgcgcg attaaacatg
    40681 cccatttcca gtccgcctag gttgatatca tgtgtcgcaa ctgcgtcccc ttcatggaag
    40741 atttcgattt taaatccagc acacggatgg atttcaacaa attcactgcc attgattcgt
    40801 ccaaaacttg ttgtgatttg tttgatccaa agatcgccga tatgacggcg gttgatcgtc
    40861 caggtttctc catttctaaa ataaaatttt agcgctgtca tttctttact cattaaaaag
    40921 actcctttat gatgtaattt attcttgtac tacctcagtt tagcacaaat ttatgtaagt
    40981 ggtcacatct tatgtttttt cttctaatga cgtatctgcc cactttaagt agccaacaaa
    41041 gctgagcaga tacagtctag tgttttttaa atcaattttt ctttcgataa aaaatccaaa
    41101 acctgttttt tatcattttt tattctttgt tctacgacgg ctttttcagc taattgcaaa
    41161 gcttcactaa cccacttacc aggttttttt ccagtttctt ctagcaagat tttaccatct
    41221 actgctaatt cgcttaatga cttgataggt aaacgatcat aggattctcc tacggcatcc
    41281 atctgaggtg tttcgtcaaa atagatcatc aattcttcta cttgcagtgc atgttttttg
    41341 cctaagaagt acaattcgaa tggttccaaa gctccagttt ctcgttgtcg tagtccttgg
    41401 acaagtgctt gaacgtcttt aatcatttgg ttagaacact tccacgattt caaaaaggaa
    41461 cgaatctcat tttcttttag atccagttgg ctgatcaata atgcccatgc ttggctctct
    41521 tctttcaatg ggacggatgg taattcagca aaacgtaaaa gtgcttcgcc ttcttctttc
    41581 aaaccgggac agcaaatgta gcactctgtt tcaataaatg catggattcc cgcggcccgt
    41641 tcatttccca agagcatctt gatgaattcc acatttatac gttctacaga tattttacct
    41701 aatagctcgt ggttttcttt gatggcgtag aaggtttctt cctctaattt gaaccctagt
    41761 tgactgacaa aacgcaaccc gcgcatcatt cgtaaggcat cttcatggaa ccgctcatga
    41821 ggattgccta cagctcgcaa gatctgttct tctagatctt gcagaccatc gaacaaatcg
    41881 atgatttctc catcttcttt caaagcaaac gcattgatcg taaaatctct tcttttcaaa
    41941 tcttctgcca acgaacgaac gaattcgaca tgatctggtc tacggtagtc ctgataagtt
    42001 gattccgtcc gaaatgtggt gatctcatat tgttcatcat tttctaatac caacacagtt
    42061 ccatgttcga tcccaatatc tatcgttttt ggaaaaagct gtttgatttc ttctggaaat
    42121 gcgcttgttg cgatatccac atcatggatt ggctggttca gcaagatatc acgaacactt
    42181 ccacctacaa aataagcttc atatcctgca ttctctaatt ttttcagaac tggaattgcc
    42241 ttttggtatt ccagaggtaa ttgttttaat ttcatttttc caacctttcc tgcgtgtttc
    42301 ttgtcctgag ataaagactc agttgcatgg tgactgtttg acaagtccgt ttcagagtct
    42361 tcaaggccag gaccacttca tcttgtttac tcgtccttac gctcaaagcg attatggtct
    42421 ctttgattga atttttccat gtttttttca aaaacttcag ttaagtcgat attcaatgaa
    42481 ttagccatga tcatcgtaac gaataaaaca tcaccaagtt cttctgctac cgagttaact
    42541 ggttcagaag cctttttgct tttttctcca taagtgtggt tgacttctct tgccaattca
    42601 cctacttctt cagttaatcg agccatctga gctaatggag agaaataccc caccttgaat
    42661 tgctggatat attcatccac ttcctgctgc atactgtata aagaacgctt tttatccatc
    42721 tttttccctc caaaactatc ttaccaaaac tttaacgcaa atcctagata agagcaattg
    42781 agaaaatcga atttccatgc tacatttagg aaggaatttc taaatggaaa ttctgtgaaa
    42841 atacataatt gaaagctgga attcatatgg tcaaacgaaa aacagtaaaa gaagttcttt
    42901 ttatcattat aggaacaagt atttatgcat ttggtcttgt ttacttgaac atcgctaatc
    42961 atttagcaga agggggagtc tcagggatca ccttgatctt aagagcttta tttggtattg
    43021 atccagctta tacgacttta ttaatcaaca tcccattgat tttactagga ggaaagatcc
    43081 tcggtaaacg ttccttggcc tatactgttc taggtacact ttctttgtca ttctttttat
    43141 ggatctggca aagggttcca ctagaaatca atttacagca tgatctactg atcgtttctc
    43201 tacttgcggg tttagttgca ggtgtaggaa gcgggatcgt ctaccgcatg ggtggaacaa
    43261 caggcggcag tgatattatc gctcggattt tagaacaaaa ttacggcatc actatggggc
    43321 gttccctatt ggcatttgat atcatcgtct tacttgcttc attaacctat atcgacttga
    43381 aacgtatgat gtatacgttg attgtttcgt atgttttcag tcgtgtgatt gattcgatct
    43441 tagatggcgg atattctgca aaaggtctac ttgtcgtctc caacaaaagc gaagagattg
    43501 caccactact gatgaccggt ctgcaaagag gtgtgacttt cctttctgga gaaggcgcat
    43561 tctctggtaa ttcaaaaaaa atcatctata tggtcgttag ctctagtgaa ttaactgaag
    43621 ctaagaggat cgtccacgag attgacgaaa aagctttcct ttcaatcatc aatgtgcatg
    43681 aagtggaagg gcaaggcttt acttatttga agccacagac aagattactg aaaaaactat
    43741 aagaaaaaag acaaagatag gctatgagga aagattccac atccagctga cctttgtctt
    43801 ttttagtact atactatttt taactaattg ttatgttcac ttacccggtt acttttcttt
    43861 tgtgtctgca aaaggatctt tcggttaact tcatcagata atttcaaaaa agcatcccta
    43921 tctccatctt ctagtgcttg atcgatttga gcataaagca tttttagttt tgcctcttct
    43981 tctttttctt gaaaatatcg ttcaattaat tcagtatctt cttcactgac tgaagaatcc
    44041 caagaagaaa atggattgtc ttctaagata gatagatatt ctcttgtctg ccaagcattt
    44101 tcgaatatac attccacata taaaggcttt ttccaattca aacggatttc atgaaaaatc
    44161 tggtctgtgt ccgtaaattc ttttccctgc aaaaacaatt tcatgggttc cccttcaaca
    44221 gatagatcac tgatctgcag tcctcgatcc gcaaaattcg ccccttcaac aaagtggaca
    44281 ttgcttaaaa tcgcttcatg attggcaaga taattcaaga tccaaaaaac ttctcttcgg
    44341 ctaaaagata catgattgac catccacgtt aaaaaattct tcttttcccg tacatcaatt
    44401 agcatttttc tcacctctct gttaggtcat ctaataatga cagtgcctcc atatctcctg
    44461 gttcaagtgt taaataataa gtcaataatt cttgcgctct tttcagctgc ccttcttctc
    44521 ttaagaaaaa ggcatactct ttcaagaaat ctggttcatg tgatagttca tgataagcct
    44581 gttcatagtg tacagctgca acggtaaagt cttctaattc attataagca tgggctaggt
    44641 tccattctgc ataaggatta gcagtttctt ccatctgatt gatcacttgt atcacatctt
    44701 catatctttc atcatccaag taaagattac ttaaagtaag taatgtttca tcttgtttgt
    44761 ctcctacttc taaagctttc agtaaaagtt cttctgctcg ctgtctatca tgcagacgga
    44821 aagcattttc cgaggccagg tgataaagtt cgacttggta aggattttct ttgattcctt
    44881 cttctaacac tgtttgtgct tcttcaatca tttcttcttc ttgaagagct tgtcctagat
    44941 atagatataa cgattgataa tgcggattaa cttctcgaag ttcttccaga tagttgattg
    45001 cctgctgatt ttctttcaat tgaagattcg taaaagctag ctggaacagt cgatcatctg
    45061 tcctttcttc tttcagtgct tcttctaaag gtacgatagc tgcttcgaat tctccttgca
    45121 tacttaagga ttgtcctaaa cgttcctgca tcgagattcc tgaaatttca tttatcccgc
    45181 ttgccaacaa tgtttcataa accactgctg cctctttgaa acgatcaact gaaaaataca
    45241 attcagctag cgcaaactgg atcaatggtt catcaggaag caacccagct gcttctttca
    45301 attttgcttc actcacttca gggattccga taacttggta caaatcagct atcgccaata
    45361 agctttgcgg atagtaatca ctttgcttat ccactttttc caggtattca aaagctaagt
    45421 cgatctcatt atcttcgatt gcaatttctg ctaacgggat gttcagtccg tcattttctg
    45481 ggtattggtt gatcagttgt tcaaaaatct gttttgcttc gatcaaaaag ccaatagcca
    45541 gcaattcttc gcctagttca gctaatatat ctgctgggtc ttcttttaat gctttttcta
    45601 aaaagatttg tgcttgtgac aagtcttcat ctgataaagc ttgcaacatt ttttcgctat
    45661 ttgtattcat ttcaattccc tcttatctca ctgacgattt gttgaaatac cgtcattgtt
    45721 ctcaataatt ctttttcgtt tggatgttgg tatccgtcta ttatacctat tttttctaaa
    45781 acaagtattt ttttgctttt ttctgccaaa aaaagaactt tttccaaata ctccatcttt
    45841 gaaatctgtt ccagccagtg gattggatag ccaagcaagt ccagccacac aaaaaagttt
    45901 tttagttgga aatcaaaagg atatgcttgg agattccaca cgaaatgaaa catcattcct
    45961 aaaaattttt tcatactagc agaaagtaga tggccatttt cggtcagata aaaagcttgt
    46021 tcaaacagtt taccataatt tgacaggttt tctgcctcta attgataaaa atagatcatt
    46081 tcttcgatca tcccggcaaa cggccgagtc gttaattgct ttttatttgg atagttttta
    46141 tacagatttt gcaaaaaacg atgatcacaa atcattccgc aacagataaa agtcaaaaag
    46201 tcaatctgct ttccgtccac ctgccgctcg ctgattgttt gatcataaac gatgcgttct
    46261 ggcagattga tggtttgcag cactacacga tttggtagct gcaaaatcgt ccgctgcggt
    46321 gttaatgctc ggcttagaga acggatcgat acagggatca tccatagtga tgaatcaagg
    46381 gttgtatgtt tttgaaaaaa acctgccaga tccatgatcc cttcatttcc aaatccgatg
    46441 ataaacatgg attgattttc gggatagcgt ttgacgaaat caagcaggtt ctttaattct
    46501 gttaaatgat tgcattgagt gttggcacaa atataccagt cattgtttgc ttgatttgca
    46561 aataacgggt tgatcttatc tgcaaacaaa tcataatagc gctgattcgt taaaaataag
    46621 attggctgat caatggaaag ttctcttagc tgactgacta gcgtttctcc gtaaaacact
    46681 ttcgtcaacg catcttctac ttgataatca agttccatac gcatccctcc tcttcttatt
    46741 tttattttac cattagtaag caaattctag aaaagataag agcaaagctc acctcataac
    46801 ttgcaaataa ataaaaatta aataaaatac atgtgttttc tatgtatgac atacggattc
    46861 ggaatctctg tctcttattt attccttttc tgctaggaaa taaaaagaaa agcacttgta
    46921 gccctagagc cacaagcgct ttttcaactg tcttatttaa cagcgtcttt taatgcttta
    46981 cctggtttga atgcaggtac tttgctagct ggaatttcga tttcttcacc agtttgtggg
    47041 ttacgacctt tgcgagctgc gcgttcgcga acttcaaagt taccaaaacc gattaattga
    47101 actttttcgc cgttagctaa agcatcttga atagttgaaa atacagcatc tactgctgca
    47161 gttgcatctt ttttagttaa atcagtagct gaagcaactt tttcgattaa ttctgctttg
    47221 tttgccatgg atagattcac ctcctccaca agagatatga tagaaatatc atttagttaa
    47281 aactttctga gtggtccaga agtttaaaaa gaaatattga ttaacttcaa tattctgtta
    47341 tcacgatatc atatgaaccg cttaatagca aggtttttac caattttcac atgctttttt
    47401 tagtcataga aacgtaacaa acgaaactgt ctttttcttt tgaaaaaaaa acgtcgatat
    47461 ggtaaaatgc ctattttctc ctacgaggga taattcggat ggacgtccct tcaaaagtaa
    47521 atgcttttct gatttgattt tctaagaaac gttcataaga aaaatgcatc aattcctctt
    47581 cattgacaaa aacgacaaat gtaggtggtt tgacagcgac ttgtgtagca taaaaaatct
    47641 tcaggcgttt tcctttatct gttggtgttg gattgattgc tacagcatcc ataatgatat
    47701 cattcaacac agccgaagag atacgaagat tctggttcat acttacttgt tcgatcattt
    47761 caggcagacg ttccaaccgt tgtttagtca ctgcagaaac aaaaacgatt ggagcgtaat
    47821 caagataccg gaattcatca cgaatctctt gttcgaaatc acgcattgta ttggtttctt
    47881 tctttacaag atcccattta ttcacgacga taataattcc acgaccagct tcatgggcgt
    47941 aacccgctac tcgcttgtct tgttcccgaa tcccttcttc agcattcagt accatcaaaa
    48001 cgatatcaga acggtcaatg gcacgcattg cacgcatcac gctgtatttt tcagtatttt
    48061 cgtatacttt tccgcgttta cgcataccag ctgtatctat cattaagaat ttctgaccac
    48121 tttcagaaac gaagtgggtg tcgattgcgt ctcgggtcgt tccttcaata tccgaaacaa
    48181 ttacacgttc ttctcctaaa atcgcattga tcaaagagga tttaccaaca tttgggcgac
    48241 caatcaaact aaacttaatc gtgtcgtcgt cctcttcttc agtctcttca gagaaatact
    48301 tcactgcttc atctaaaata tcaccaatcc ctagtccatg gcttcctgaa acaggataag
    48361 gatcgcctag ccctaaggaa taaaattcat agatatcgtt tctcatttct ggattatcta
    48421 ctttattgac tgctaaaatc actggtttat tacttctgta taaaattttc gcaaccatct
    48481 catctgcgtc tgtcacgcct tcgcgtccgc tgactacaca aatgattaca tctgcttctt
    48541 caatcgcgat ttctgcttga tgcttgatct gatccataaa aggttcatcg ccaagatcaa
    48601 ttccgcctgt gtcgatcacg ctgaattctc gacctaacca ttcacctttt gcataaatac
    48661 gatcacgagt tactccagga gtatcttcga caatcgagat acgctcacca gcgatgcggt
    48721 taaaaatcgt agacttaccg acgttcgggc ggccaacgat tgcaattgtt ggatttgcca
    48781 tattatttcc tcctcacaag tttccaatct cacttagttt aacgaatgca ttgccaactt
    48841 tcaagcatag attgtcaatt aaacgaagta tttttattcc ttattgaaat actaatcgct
    48901 tttcaaaaga aaaactgaga caaaagcagc tgctttcatc tcagtcacag atacaaaatc
    48961 aattattttt cagaatcatc ggttgctttt tcttctactt cttctttcaa cgcatcacct
    49021 aaaatgtctc ccatagtaaa tccagtattt tcttctggta attcataaga ctcaacattt
    49081 tttggttctt cttctgaagc tggtttttct tctaaagctt tgatactcaa tgcaatacga
    49141 tgttcttctg gatggatctc taatactttc actttcactt cgtcaccttc atgaagcact
    49201 tcatgtggtg tcgcaatatg tttatgtgaa atttgagaga tatggactag cccttctact
    49261 ccagggaaca cttcaacaaa ggcaccgaag cttgtcaaac gtttaactgt tccgtctaat
    49321 actgtgccaa ctggagcttt tgtatcgatt tctgaccaag gtccaggctg tgtgtctttg
    49381 attgacaagg aaacacgttc ttgttcagga ttgatagaca acactttgac atttacatca
    49441 tcattgacag ccaatacatc acttggtttt gctacatgac tatgtgaaat ctctgagaca
    49501 tggactagac cgtcaattcc gcctaggtcg acaaatgcgc caaaatcagt aagacgtgca
    49561 acacgtccac tgagaacatc tccctcatgg atagttgaaa gaatttcttt tttcttttct
    49621 tctctttcgt tttccactac cgctttatgc gataagatca aacgattttc tgatggttcg
    49681 atttcgatga ttttaaagct caatgttttg cctttatagt ctgaaaagtc tgcaacaaaa
    49741 tgatcttcca ccattgatgc agggacaaaa ccacgtacac cgacgtcaac gactaaaccg
    49801 cctttgacaa catttgtaac aggtgcttcg atgaccttgc cctcttggaa atctctttcg
    49861 atatcttccc aaactttttt agcatctaaa cggcgttttg aaagtagata gctgccgttt
    49921 tctttatctt tgccgatcgt actgataacg actaaatcta atacatcacc gacttttact
    49981 acttcgctga tatcttctac cggcaaagtt gataattctt ttgccggaac gactccttca
    50041 actccagccc cttcgattcc gacaatcact tgtttgtctt caatgactaa aacttcgccc
    50101 ttaacaacat cgccaacttt tacttcttgt acgcttttca ttgcgtcttc cattgactgg
    50161 ttgttctctt gattttgatc aaattctgtc ataacacttg tcctcctaac caacattctg
    50221 ccgtaagtac ggtactgttt ttagtttaat tgattattca aaaaaaataa agcgatttcg
    50281 ttcgttttcc ttgaaaaaac aattgttttt catttttttt cttaatttaa aaaagaagat
    50341 aacctttttt caaaacaaca gcttttattg ctgcaacgac ttctggaatc gatttgccag
    50401 tcgtatcgat acgaacagca tcctctgctt gcttcagcgg agagacgtcc cttgttgaat
    50461 cataatggtc tctttcctgt atctctttcg tcagtgtttc aagatcagtc ggaattcctt
    50521 tttcttggtt ctctttgaaa cgtctttctg cacgctcact tacgcttgct actaaaaaga
    50581 ttttcacttc tgcattcggt aatacagctg tcccgatgtc tcttccgtcc atgacaacgc
    50641 caccgcgttc ccctattttt cgttggacag caactaattc ttctctgact tttccatgtg
    50701 cagagacttc agaaacagct tttgtgacat ctggttgacg gatttctaaa gtcacatctt
    50761 ttgatgctat gaatacatgc tgtccatctt cttgttgcga aaaagagata ggatactttt
    50821 tgatcagcga aactagccct tcttcatctg aaaaagatac attgttcgta atagctaaat
    50881 aagttactgc gcgatacatg gcaccggtat ctgtataaat ataattcaat tcttttgcta
    50941 atatcttggc aacggtactt ttcccagatg aagcaggccc gtcgattgca atactgattt
    51001 gtgtcatgat ataactcctt tttctcagaa aaaaagccgc aaagcgcggc tattattatt
    51061 taattcttaa ttcttgacca gggtaaagca taaagttgtt tggatctaag ccattcaatt
    51121 caaacaattg atcagtggtg atccctgcac gctcagccac ttgttttgga ccttcgcctg
    51181 cttgaacagt cgttgttgaa ccggctgcag ctgatgaaga aggtgttgtt tctgctgcat
    51241 tgctgcttga atcccctgct tgataagttg cctcacttga ggaagtcggt gttgtctctg
    51301 ctgcaatact gctttctgac gttacacctg tatcttgaga cacagtagat tcttccgcga
    51361 ttgtactaga ttctactgtt gaagtctcag ttgactcctc ttttgttgta cttgtagaac
    51421 tttctactac tgatgtcgaa gtttcagaag aagttgctgc tgttgcattg ttgcttgatc
    51481 catttctgat ccagaaataa gcacctgctg gaatagcgat acataagacc agtaaaatca
    51541 aaaatagcga aagaaataat gtatttcctt tcttttgttg tctctgagaa cttcttgatg
    51601 aaacttcatc atctgtatcg tagattggtt gttcccatgg ttcttgtgtt tcgttatttt
    51661 ctgtagattg atgtctatct tttctgctca ctgttttacc tcctaaactc tatcgtgtgt
    51721 attataccat aggaaaaagt gtcttgtcct aaaaaatcaa tgttccatgt tttttttaaa
    51781 taaatttagt aaaatcgttc cccaatgaag ttgttcagtc ttagaaaaaa gcttcctctt
    51841 tggcagaagt tctgcttttg ctccatcaat atcacaacaa ttctgcggct tttcggagag
    51901 tttttcgcca aaataagcta agatgaattt tctgcggcag ttttcttcat taatatagcg
    51961 aagcatctgc tgcaggcgga attctttttc tttttcctga cgtttgagcc gttccaccca
    52021 attttctggt ttttcagact gctggatcag ctccatccat tttttttgca gctcatcaaa
    52081 gggagcttgc tctgtggcat attccaaata aagctcaaaa ctttgtcgct gttctctaga
    52141 aagttgatta aagaaataat ggatacgttc atccccactt tgataaaaaa gaattgctgc
    52201 actttcttcc tgatctctgc ctgctcgacc gatttcttgt acatagtttt ctagactgtc
    52261 tggcaaatca taatggacca cataacggat atcagattta tcgatcccca taccaaatgc
    52321 attcgtagca atgagaaact gcagctgatt ttttacaaac tgctgttgaa gttgtcttct
    52381 ttgagctgca tcaagaccac cgtgatagta tccaactgta aaacgttccc tgaataaatg
    52441 atacaatcgt tcgacttctt tttttgttgc acaataaatg attgccgctc cttgtgctct
    52501 ttccatgaag tgttcaagtt cttgttcttt ttgctgcgtt ttccgaacaa aaagcgagat
    52561 attttgccga ttgactgatt gtctgatctc ttttggtggt cgatcaaata gtacatgacg
    52621 tatatctctt gctactaaat ctgttgctgt cgccgttagt gccagtgtga ccgggtttcc
    52681 taaaagcttt cggatttttc ctaactgttg atattctgga cggaaatcaa ctccccattg
    52741 agacacgcaa tgagcctcat ctactacata taacgcaata ttttgttttt tcagatgctc
    52801 taaaagagaa ggattagtca acatttcggg actcaaaaac aaaaatttat attgcgatag
    52861 atgacggagg acgtatcttc tttcagattg cgaaagtaaa ctattgaaag cagctactcg
    52921 tttctcccct cgtttttgta attgcgtcac ttgatcttcc attagagaaa gcagcggtga
    52981 cacgatgatg accagacctt ccattaaata ccccgtcaat tgatagcaaa ggctttttcc
    53041 cgttcctgta ggcaaaatag cgagagtatc ttctccatca agcaacgcct gaataatttc
    53101 ttcttggccg ggacgaaatg aatcaaatcc aaaatatttt tttaattcat ttattaacat
    53161 tctgtatcgc ttcctttctc aatagataga tttgatacaa acgaaattct ccataatcta
    53221 attgtccatc tacattccat tcgctaaagc gccactctcg cacggaatcc ttatttggtt
    53281 ccaaacgttc gattgtttct gcagagagaa tccactcgta tggaaaatca tcaaaaaaca
    53341 aggcccactc aataagatga tctgttactg ttcctctttt taaatgacgg attgccatga
    53401 tctggtcaat tgtctgacca ttcataaaca tctgcttggt gatcatcatg ctttgattga
    53461 aattttgctg taataatgaa tcgatcaaag cgtgccaaag cgaatctggc cgtttttcga
    53521 tttgtgctaa caaaaggtgg atacatctgc tttgttgcag ataaaccgct gactcatcat
    53581 tgttttttgc cagctgataa ggaagcagcc ctgttcgctc atttccagat aattggttag
    53641 ctaaaaaatc tgctgcttct tctggtatct ccgaaaacag ctgcaccaaa ctctcatacg
    53701 cagtatcaat caaaatttca cgtggcaatt ggctttcact aagccatttt tttagttgga
    53761 aagtataaaa aggacttgtc tctgctggaa gatagtcttg aatacccgtt gccagattag
    53821 aaattacttg aacagcaaat ttaatgagcc gccaactagt ttcccctgtt ctgccatagc
    53881 ggtcaaaacg caaaccagta tgttctattt gatgttcgga caacatatca gcccctgcag
    53941 gcgttagctt tgcttcattt tcatttattt cgatccaacc ttgctgcgca attttttgca
    54001 aaatttgata aaacttatcc tgcttcaatt ctggaaaact gttatggata aacaaaagtt
    54061 cgtgggtaaa tccataaata agtactgaag tcgttcgttt tcccacaagg agttggtaaa
    54121 gagaactaat ccgcagcttg tgtcctgatg aaaatagcga taaaataaaa acaatcatat
    54181 tattctcctt aagttaggat ggttagaaat gtcactacta tgtaaacttg tacctgaacg
    54241 ctgtattgca tgcggtcttt gtcaaatcga agcgccagat attttcgatt atgatgaaga
    54301 aggaatcgtt ttatttgccc aagaaccaga ggctcatcaa cagttcgtga tgccggaaga
    54361 agaaacatat gtaaaaaaag cgtatcaaaa atgtccagtt cgagcgattt tattagaaaa
    54421 taaaatataa atataaacga aactttaaaa acgtacacta agttctctaa acttgaaaat
    54481 aagccgggat ctgtttctct tgttccgtga aatcccgact tattttgaaa gattcatttg
    54541 ctatctatag ttaattgact agttttttca cggaccctat aggacccatt tatattattt
    54601 caactttttc agccagtttt ccaatgtatt gacgatcaca gtttgctggt ctgcgttaga
    54661 aatttctgcc tctccatctc ctttttgctc accatagctg ccaaaacctc catgatttcc
    54721 gccattgatt gatacatact ctgttctttc cggtaaatac tgcttagctt cttgataagc
    54781 ttcctgattc aatactttat cttttgtcgc cgtgatcgat aaaacaggaa cagctgattc
    54841 tgtcaatgtt ccttttttgt cgggataact tgccatgaag aatacccctt ttaaatgttt
    54901 gtctgaatga ttcttattga cataacgact tgccattacg ccacctaatg aatggccacc
    54961 gatcacataa tcggaatttg gaaaatcttc tttgacctta tccgctttgt ttccgcccat
    55021 cactgctaga tcaagtggca ttttgacaat agcgactgaa aagccgtttt ttgctaaatc
    55081 ttgcgcccaa atactataac tttctggctc tacgagagca ccaggataaa aaataagtaa
    55141 tggagacttt ccatgattct ttttctctcc ttcaaaaaat aagtaatctt ctttgttttt
    55201 tccttctttt accaacactt gcgcttctga cgatggttcg tacgtatttt gctgtatata
    55261 gtaataaccg gctccaccga gtaatcctaa aatgatgact aagccaatta gtattcgtct
    55321 aaatattttc actttctccc accttttcta tctgagtgac ccctttttct taatcaagta
    55381 tgattatacc ttaaaaaaat aagagagaaa tgcaaacttt ccagcatcaa ataaaaaaag
    55441 acggaatatc catttgaata tccgcccttt ttgtatgttt atttttattt agcacataaa
    55501 cgatacacag cttctgcata aatttccatt gctttataca tactacttaa ttcccatcgt
    55561 tcattcgcct ggtgcataaa gtcaggagta tcagggaaca tcgcaccgaa tgccacacac
    55621 tggttcatcg tacgagcaaa agtagcgccg cctgatacaa acggttcagt catgtctcca
    55681 gtaatatcgc gatatgtccc caatagattt ttcactaact cactgtctag tggcacgtat
    55741 aatgaatcaa ggaagtcaaa ttcttcataa gtcaacccat atttttctgc tgttttagtt
    55801 aatttagcta ctaaatcatc tttcttgaat gtaacaggga ttctcatatc tacaccgatt
    55861 tttgtttgtt ctggtgtgat ttccaaactt gcaaagttca tcgttagctc gccggattgt
    55921 tcatcttctg ttttgcccac gacagcttca ccagttgcat tttcttgaac taattttcct
    55981 aagaaattga ttgggccaaa atcaaatact tctgaaagag caattgccag acgagaaata
    56041 gcattcactc cttgtgctgc atctttcgcg tggatacttt taccaagaac gacgatgcct
    56101 tcaccttgtt cttcgaaatc gaattcgtgt tttttcaatg cttcttttac ttcagaaagc
    56161 ttttctccag agtaaggagc tgcatcagga acaacattca acgctcctcc agcttttaca
    56221 gaaaactcgt tcgttcctgg tcctgtcaag taagcttgca ataagccttt ttctgcataa
    56281 attaaaggaa attcagcatc tggcgcaaat ccttgcgtaa ttccttcttc tttttcgtta
    56341 tatttttcta aacaacgcca taaattttct tcatctgtac caaagataaa acgaatacgt
    56401 gtcttgaatt ccacgccagc atcaatcaaa gctttgacgg catacattgc cgcaatagaa
    56461 ggacctttgt catcttggga tccgcgacca actaaccagc cgtcttttat agttggatcg
    56521 aatggttttg tttcccaatt attttcgtcc cctgctggaa caacgtccat atgacacaag
    56581 ataccgaata attcatctcc agagccaatc tctgaataac cgtaatagcc ttctggatct
    56641 ttaaatgttc taaatcctag cttttcactg atttccaata ctttatccaa agcttcaatc
    56701 acttttttac cgaagggatg tccttgtcct gaatctgctt catctagtac ggaaggaatt
    56761 cggatcaatt cgctcaatga ttctaatgct tgttcatgat gttgttctgt cacaaatttt
    56821 ttcatgatat tatcctccta atatgttaaa aagggtcatg tcaaattcct ccgacacaac
    56881 ctcttcttct caaataatga cgttttaagc ctgctccatc agcaagcaga atgttcttaa
    56941 caaatgatga aagaaaaaac actcactgaa tggagctatt gacttatcca cgtgcttatc
    57001 gatagccgtt tatcggtttc ttcattctta tgatagacct tgtacctgta tttgtctagg
    57061 tttagaacat tgctgctaca cctaggatca cacagatggc tacaaataat atcgcaatta
    57121 atttaccggt aaatttccac cacactgaaa catcgaaacg agcaattgct acagcaccca
    57181 ttacgatagc agatgttggt gtaattaagt ttactaaacc agatgcagat tgataagcag
    57241 tgattactaa atccttaccg acgccggcaa attcacccat aggtcccatg attcccattg
    57301 ttgcagcagc taagccggaa gttgaaggaa tcaagaatga catagggata tagaaaataa
    57361 atgtaataat gatgaataca cttgaagata atgaacttaa tccttgttcg ccccaatgta
    57421 agatggtgtc tgtgattaat ccattgttca taaccacttg gataccacga gcaacagcta
    57481 ctacgattgc tacaccgatc aaatcagaag caccagctaa aaattcagag acaaattcag
    57541 actctttcat gccataagtg attgctacta cgatggacat tactaagaat aacatagtaa
    57601 tttctgggaa gtaccagtca cctaatggaa gcatgctttt acctagaacc gtacctagta
    57661 ccggaaggtt tgttaaccag tttgtcaatg attcaaaaat cgtgaaattc gcattcaagg
    57721 ttgtccaagg aatcaaactg atgatcatga taccaaaagt aataaagaac aatacattaa
    57781 catgtttttg ttttttcgtg attacttctt gacgaccgat tgcttcaata tcgaaatgct
    57841 tcatatcttc tttacgttgg ttgtatacta atgattttgt tggatctttt tcgatctttg
    57901 atgcataacg atacacatat acaatagaaa tagctaacaa aataacgaac atcaagaatc
    57961 gaagaccaat accttcaccc atcgaaatcc caacagcttg tgaagctacg cctgttgcaa
    58021 aaggatttac cgttgaagct agacagccta cttgagaccc tactaatacg attgctacag
    58081 ctactactgt atcaaagcct acagccatca tgactgggat cagtaaagca tagaatggaa
    58141 tcgtttcttc tgccattccg taagtagagc cgcctaatgc aaataacagc atcaatactg
    58201 gaataagcat tttttctttt cctttgaatt tcttcacaac tgaagcaatc ccggcatcta
    58261 gtgcccctgt tttgttgata atacctaaaa atccaccaac aaccaaaatg aagaaagaga
    58321 ttgggattgc agcaggtgtt tctttagtta gctcccccgc tggtacacct agcatcccat
    58381 tgattggcgc catgaaaata tcccacagcc cttgaggatt ttgttgtact cgttcatatg
    58441 atccagcgac gatgttgcct gcatcgtcta cactatagtg cccagctggt ataaaccaag
    58501 taagtacagc gattactgca ataataataa ataagaccgt atacgctgaa ggcatttgaa
    58561 attttttctt ttttgtttgt tccatcttct tcttcctttc ttttccgtta gagacaacaa
    58621 ttttggctct aatgttattt tatcaaaaag agtatcagaa caaaaataat aacacgcatt
    58681 ttgtaaaact tttgttcaaa aagttcgttt ttccctattc cctcctcttt tttcaggttt
    58741 ttaaagcttt cttctttttc attgtagcgt ttcctttttt ttataaacaa acattttcgt
    58801 cttttaaaag gagaag
//
LOCUS       DAZKOK010000004        55496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000004 DAZKOK010000000
VERSION     DAZKOK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.004"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..312)
                     /locus_tag="ACG90W_000243"
     CDS             complement(<1..312)
                     /locus_tag="ACG90W_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0476640.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TS"
     gene            complement(442..1026)
                     /locus_tag="ACG90W_000244"
     CDS             complement(442..1026)
                     /locus_tag="ACG90W_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0476641.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1357..1590)
                     /locus_tag="ACG90W_000245"
     CDS             complement(1357..1590)
                     /locus_tag="ACG90W_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0476642.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1605..2555)
                     /locus_tag="ACG90W_000246"
     CDS             complement(1605..2555)
                     /locus_tag="ACG90W_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0476643.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2552..3394)
                     /locus_tag="ACG90W_000247"
     CDS             complement(2552..3394)
                     /locus_tag="ACG90W_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0476644.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3396..4115)
                     /locus_tag="ACG90W_000248"
     CDS             complement(3396..4115)
                     /locus_tag="ACG90W_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0476645.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            4663..4953
                     /locus_tag="ACG90W_000249"
     CDS             4663..4953
                     /locus_tag="ACG90W_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0476646.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            5025..6251
                     /locus_tag="ACG90W_000250"
     CDS             5025..6251
                     /locus_tag="ACG90W_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0476647.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(6339..6731)
                     /gene="rpsI"
                     /locus_tag="ACG90W_000251"
     CDS             complement(6339..6731)
                     /gene="rpsI"
                     /locus_tag="ACG90W_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0476648.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6747..7175)
                     /gene="rplM"
                     /locus_tag="ACG90W_000252"
     CDS             complement(6747..7175)
                     /gene="rplM"
                     /locus_tag="ACG90W_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0476649.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7709..8176)
                     /locus_tag="ACG90W_000253"
     CDS             complement(7709..8176)
                     /locus_tag="ACG90W_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0476650.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8296..9834)
                     /locus_tag="ACG90W_000254"
     CDS             complement(8296..9834)
                     /locus_tag="ACG90W_000254"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0476651.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9914..10600
                     /locus_tag="ACG90W_000255"
     CDS             9914..10600
                     /locus_tag="ACG90W_000255"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0476652.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10801..11328)
                     /gene="msrA"
                     /locus_tag="ACG90W_000256"
     CDS             complement(10801..11328)
                     /gene="msrA"
                     /locus_tag="ACG90W_000256"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0476653.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11412..12089)
                     /gene="pgmB"
                     /locus_tag="ACG90W_000257"
     CDS             complement(11412..12089)
                     /gene="pgmB"
                     /locus_tag="ACG90W_000257"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0476654.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12086..13216)
                     /locus_tag="ACG90W_000258"
     CDS             complement(12086..13216)
                     /locus_tag="ACG90W_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0476655.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13216..15399)
                     /locus_tag="ACG90W_000259"
     CDS             complement(13216..15399)
                     /locus_tag="ACG90W_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0476656.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15396..16421)
                     /locus_tag="ACG90W_000260"
     CDS             complement(15396..16421)
                     /locus_tag="ACG90W_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0476657.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16466..17293)
                     /locus_tag="ACG90W_000261"
     CDS             complement(16466..17293)
                     /locus_tag="ACG90W_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0476658.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17290..18357)
                     /locus_tag="ACG90W_000262"
     CDS             complement(17290..18357)
                     /locus_tag="ACG90W_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0476659.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18383..19219)
                     /locus_tag="ACG90W_000263"
     CDS             complement(18383..19219)
                     /locus_tag="ACG90W_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0476660.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19216..20085)
                     /locus_tag="ACG90W_000264"
     CDS             complement(19216..20085)
                     /locus_tag="ACG90W_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0476661.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20155..21438)
                     /locus_tag="ACG90W_000265"
     CDS             complement(20155..21438)
                     /locus_tag="ACG90W_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0476662.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21452..23137)
                     /locus_tag="ACG90W_000266"
     CDS             complement(21452..23137)
                     /locus_tag="ACG90W_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0476663.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23412..24437
                     /locus_tag="ACG90W_000267"
     CDS             23412..24437
                     /locus_tag="ACG90W_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0476664.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(25032..28685)
                     /gene="rpoC"
                     /locus_tag="ACG90W_000268"
     CDS             complement(25032..28685)
                     /gene="rpoC"
                     /locus_tag="ACG90W_000268"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0476665.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(28764..32381)
                     /gene="rpoB"
                     /locus_tag="ACG90W_000269"
     CDS             complement(28764..32381)
                     /gene="rpoB"
                     /locus_tag="ACG90W_000269"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0476666.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNSGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            33109..34077
                     /locus_tag="ACG90W_000270"
     CDS             33109..34077
                     /locus_tag="ACG90W_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0476667.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            34095..35000
                     /locus_tag="ACG90W_000271"
     CDS             34095..35000
                     /locus_tag="ACG90W_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476668.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34997..35803
                     /locus_tag="ACG90W_000272"
     CDS             34997..35803
                     /locus_tag="ACG90W_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0476669.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            35960..36928
                     /locus_tag="ACG90W_000273"
     CDS             35960..36928
                     /locus_tag="ACG90W_000273"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0476670.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(37245..37769)
                     /locus_tag="ACG90W_000274"
     CDS             complement(37245..37769)
                     /locus_tag="ACG90W_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0476671.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            38056..39753
                     /gene="scm"
                     /locus_tag="ACG90W_000275"
     CDS             38056..39753
                     /gene="scm"
                     /locus_tag="ACG90W_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0476672.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSS
                     TTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDN
                     RTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(39908..40894)
                     /locus_tag="ACG90W_000276"
     CDS             complement(39908..40894)
                     /locus_tag="ACG90W_000276"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0476673.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(40917..41483)
                     /locus_tag="ACG90W_000277"
     CDS             complement(40917..41483)
                     /locus_tag="ACG90W_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0476674.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(41497..41913)
                     /locus_tag="ACG90W_000278"
     CDS             complement(41497..41913)
                     /locus_tag="ACG90W_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0476675.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(42370..44304)
                     /locus_tag="ACG90W_000279"
     CDS             complement(42370..44304)
                     /locus_tag="ACG90W_000279"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0476676.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            44606..45541
                     /gene="pplA"
                     /locus_tag="ACG90W_000280"
     CDS             44606..45541
                     /gene="pplA"
                     /locus_tag="ACG90W_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0476677.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            45631..46734
                     /locus_tag="ACG90W_000281"
     CDS             45631..46734
                     /locus_tag="ACG90W_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0476678.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            46747..47700
                     /gene="menA"
                     /locus_tag="ACG90W_000282"
     CDS             46747..47700
                     /gene="menA"
                     /locus_tag="ACG90W_000282"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0476679.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(48514..49860)
                     /gene="gor"
                     /locus_tag="ACG90W_000283"
     CDS             complement(48514..49860)
                     /gene="gor"
                     /locus_tag="ACG90W_000283"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0476680.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(49994..51247)
                     /locus_tag="ACG90W_000284"
     CDS             complement(49994..51247)
                     /locus_tag="ACG90W_000284"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0476681.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(51253..52176)
                     /locus_tag="ACG90W_000285"
     CDS             complement(51253..52176)
                     /locus_tag="ACG90W_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0476682.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(52397..54889)
                     /locus_tag="ACG90W_000286"
     CDS             complement(52397..54889)
                     /locus_tag="ACG90W_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0476683.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(54896..55363)
                     /locus_tag="ACG90W_000287"
     CDS             complement(54896..55363)
                     /locus_tag="ACG90W_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0476684.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT        16265 a        11580 c         9946 g        17705 t
ORIGIN      
        1 gctagtatag acactaacca acaaccatga gatcattacg accggcaaca ttcgaaatac
       61 aatatataag gatgtcgcaa aaaatatagt aaaaaactct atcttgatag catcaataat
      121 tttcattact aaaaacagaa ctgaataaat caagatgaaa taaatacatg tctttatttt
      181 acgaaacatc aatactaaaa ctgtagcaat aatgaaacac cccacttgaa atatatttcc
      241 ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt
      301 gatttttgac actctttcac ctccaaaaaa taagatcgca ctgttatctt aagctgataa
      361 aaatgcaccg ttattcagat aacagtgcga tcttatcaaa aagcataact gagaacaatt
      421 ctcaatgcaa gcttttttta attaactcag taattcaatc cacccaggaa cagaaaattg
      481 atacaatctt ctggttaata gcatagtctt ttcttttgaa tagctatgct tcaatatatc
      541 atagaatgta gcgtagaatg agtaaatgta gaagtggatt tcttcttcat taaagactaa
      601 attttgattg atcattccga tagtctttaa cttatctaga caacagactg tctttttcgt
      661 cacaaatgat gtaatttttt caacaatatc ttgatatttg gttccagctg attggaataa
      721 aatcaaatcg aatatacgtt tattctcaaa aatataatta ataaatggat ctaattcatt
      781 gaagttctct ctccaacttt tctctatatc tatattttct aagtttaaga ggtatttggt
      841 aaactcttca ttatacatat taataagcgc attaatatgc ggctgaataa tttcttcaaa
      901 taacgcttcc ttagaagtaa aatgtttata aaaagcaccc gtagttacgc ctgcggtcag
      961 gcacagttgc cttatcttag tttgctgaaa tccttctttt tcaaaatggc ttagtccact
     1021 ttctattaac ttttggtgag ttataatata atctctggtt tctatgggta cttcctccta
     1081 ataagatgaa ttaactttat tatcttacac aggacttgaa gtttcaatcg ttaacgttta
     1141 ttactgaact cattccttag agctaaacta atgagaaaga gcagaataac tgatacaaaa
     1201 agcaatccta caagaattaa tagactcatc taaatcattc accttttcta aaataatccg
     1261 agaaagattt aaaaaaatta tatccagcta ttaggataag tcctatcata atcactccaa
     1321 tagccatttt cttatcctcc ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc
     1381 gtcaataaat tttttccctt tatcagttaa ttgaaatcct atatctaaat agtcctcata
     1441 tctaagttta gccagcatct cacttacaga tgctggacta acacttagaa cttttgctaa
     1501 atcattattt tttaatttct tttttctttt tgataattcc tgaattgcta gtaaataaat
     1561 tagtatagta gtcgttttaa tttgtaccat acctaccctc caaattattt cgaaaggcct
     1621 tcagcaattt tatccaggtt ccatttcatc atcgcatagt aactatcacc atcttgtcct
     1681 ttttctgcaa ttgaatcagt aaaaatcgtt gaatagatag gaatattggt atcttttgat
     1741 actgttttca ttggtctatc atccacactg ctttctacga ataaggaggg aacttttgtt
     1801 gtgcgtaatt tttcaactaa gtgttttatt tgatctggtg ttccttcttc ttcagtgttg
     1861 atttcccaaa tgtaagcaga aggcacatta tacgctttcg agaaatattt aaagcatcct
     1921 tcacttgtaa caatcatttt tttatcattc ggaattgaag caaatttaga tttagcttct
     1981 ttgtctagag aatccaattt ttcaatatac ttatctagat tttctttata gaatttttta
     2041 ttatcaggat ctttttccgc taactgtttt tcaatatttt tagcgtaaat aataccgttt
     2101 tctaaattta accaagcatg aggatcttcc ttccctttct cactctgacc ctctaagtaa
     2161 ataacatcta tgccatcact tgctgcaaaa tagtctttgt tttcctcttt gttcgcattt
     2221 ttaactaatt ttgtaaacca agcatttcct ccagtttcca agttaacacc gttataaaaa
     2281 atcaaatctg cttttgaagt tttttgaaca tcttcaggca aaggttcata ttcgtgggga
     2341 tctttcccaa taggtacgat actgtgtaaa tcaattttat cttttgctat attttcggta
     2401 atatctgcta aaatcgaatt agtaactact acttttaatt tttcgttcga agccacttta
     2461 tctttgtctt tactatttat tgaacatgcc ccgaatataa aaagagcaat caaaatcccc
     2521 agacttccca gtactgcttt ccattttctc atcataacga tctctccttc tttcttaaaa
     2581 atttttgttt tggtgagatt agaaaactaa ttacaaataa gaatgccgct gttaaaacaa
     2641 tacttgatcc tgctgctatg ttaaaactat aaccaataaa caatccaagc aatgatgaac
     2701 ttgctccgac tgttgcagaa ataaatatca tagttttcaa gctatttgca tataaatatg
     2761 ctgtagctgc tggtgtaatt aacattgcaa caattaatat agttcccaca ctttgcatag
     2821 ctgttactgc tactaaagtt aatagaacca tcaatagata atgataaaaa ctgacattca
     2881 tccctattgc ttttgccatt aatggatcaa aagaagtgat taataattct ttaaaaaata
     2941 gaactactat aaccaacact aaaaccgaaa cgccaattgt aatccactta tcaatatctt
     3001 gtacagctaa aatatttcca aataaaatat gaaataaatc cgttgagcta tttgcaactc
     3061 ctataaggat aactcctagt gctaaaaaag aactgaaagt aataccaatt gctgtatctc
     3121 cttttattac actattgctt tttataaaag taataatgat agaagctaat agaccaaata
     3181 caatcgcacc aataaaaaaa ttaataccta aaatataaga aattgctact cctggcaaaa
     3241 cagcatgaga aatagcatca cccataagag acattcctct taaaataata aaacaaccta
     3301 ctgttccaga aacaatccct ataactacag atgtaattag agcattctgt aaaaagtgaa
     3361 agtcatacag accattaata aaattttgaa gcatattatt ccccctctcc aataaatata
     3421 gtatctccat aggctttttt taaattttct tcattaaata cagattctac acttccgtga
     3481 gcaattagat ttcgattcaa aagaataata ttatcaaaat acttctttac cttgcttaaa
     3541 tcatgatgca caattagcac tgtttttccc tgatgtttca atttttttaa cgtatccata
     3601 attattcgtt cactaactaa gtctatccct acaaatggtt catctaaaaa tatgaaatcc
     3661 gcattttgag caagacatct tgctaaaagt acacgttgaa attgtcctcc agataattct
     3721 cctatttgat gattagaata ttttcccata tcgacctttt ccaaagcctt tgaaactctt
     3781 tgccattctg catttttaat tctttgaaaa actttcattc cggcatatgt tcccatagat
     3841 acacattctt ttacttttat tggaaaagta aaatcaatat cagctttttg ctctacatat
     3901 gcaatcttca ccagtttttt attgatatct tcattattaa tggagacctc tccctgatga
     3961 gggataatat ttaacattgc tttaagcaat gtggattttc cagcaccatt tggaccaata
     4021 atacctgtta aagcaccttc ttgtattttt aaagaaatct cgttcaatgc taaaaaatcg
     4081 ttataacaaa cattaagctt tttcacttct aacataactt tctccttcta aatgataatg
     4141 attatcacga tcatatggta tacaaaaatt atattttagt caaccctaaa ttttatagtt
     4201 tagatttttt tctgttgctg aagattagaa tgacaaaact atttgaaact acgcgtatag
     4261 taagtagctt tcaaaatata taaaaaagga gcgttctcaa atgagaacat gctcctaaaa
     4321 ctactttatt ggcgtatgga aaaattatac ttgaagtgga tagcgggttg aataagttga
     4381 taagcatcaa aacatttata aaaattattc aagactatgt ctgttccata tacaaatatg
     4441 aaaatcagta aactccattt aacttttttc gtagtgtgtc tttttataca tgtttatcgt
     4501 tattaatagt gaataggaaa aaaactattt ttgatcattg acaattaaat acaatttttg
     4561 aataacgatc cctgctattc gagctagaaa ctcaagtacg ccatgacctg attagcgaca
     4621 aatacttaga gatttctttt tgttctggga aaacaaaatg ccatgacaat agcagcaaat
     4681 aatgatactt ccgtaaggct cgccgtaaac tggggagagg tgaaattcct atgtgcttac
     4741 ccaaagcaat tcaaaaattt cataactact attttaggag atgattttat agaatccaga
     4801 gtctacctga ataaaaaaca aacctgtgca tttctaggaa tcgataataa tactctagat
     4861 aagtggattc gtatatataa tttcccagta ataagagttg gtggtgtagt tcgctttgac
     4921 gtagaagata ttaaagactt tatgaatact tgaaattttc ttctcgccgc atatactaaa
     4981 attagtgaaa tatactgcct tacttttttt aaggagtgag gattttgcct acaaagcttt
     5041 caaatgggaa atataaaact aatctgagat atccaaaaag gtttaaagaa ataacaggaa
     5101 ttgcatcaga aaagtatcaa aaaacatttc caaataggca attggcgatt aaagccgaaa
     5161 atgatatgaa gaagaaaata gaaaaagttt tgagagagga gaatgcaaac tcattagaat
     5221 taaaaggaaa aataacgttt aaaaaatttt acgaatctaa atggcttcct agatatgaat
     5281 taggtcaaac aattagatca aacaggcctc ctagtgatat aaccattagt aatacaaaag
     5341 atatatttcg gttgcatatt ttacctatgt ttggtgaata cgcaatgaat tatctaaata
     5401 tcaatactga gataatatca gatgaattaa ccaaaaaatc gaaagaatat gcaaatataa
     5461 aaatcataaa aggatatgtt cgttccatgt tcgatattgc agaaatacta aattatatcg
     5521 aatttaatcg aactactaaa attattcaaa gtatcactgc acctaaaaaa aatgctttag
     5581 aagaaaaaag aatacaagaa ggtaagcaag cactatcatc aaaagaactt actaattgga
     5641 tagaagcagt aaatgatgat tttaataatc atttacttac ttttcatgat tacacacttt
     5701 ttatgattac cctgtatctt ggtgatagaa aaagtgagac ttacgctttg caatggaaat
     5761 atattgattt tgaaaaacaa acggtccgtc ttaaacatac tttagataaa tatcaaagaa
     5821 aaaagtttac aaaaggtcga aaagataccg tcattcaagt accagaagtt gtaatgacat
     5881 tgctaagtga atggaaatcg gtacaagctg atcaattgtt gaaattaaaa attaagcaaa
     5941 cgttagatca gtatctcttt acttatacta aaccatctgg agaagtaaat tgtcctgttc
     6001 atgccgatta cctaaactat cgaatcaatt caattaaaag aaggcatcct gacttagttc
     6061 atttatctcc acacaaactc cgtcatactt atgcaactat agctagacaa ggtggagcag
     6121 atatgaatca aatttcaaat gctttaacac actcagatat ttctacaaca aagatatatg
     6181 tgaacacacc agatatagtt gataaggctg tttttgaagc ttttcagaga ggattgaata
     6241 agtgtgattg atatgtgttc ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat
     6301 aattgaaaag tgccagaaac gttgatatat aaaggatatt aacgttttga gaattgtgaa
     6361 gctttacgtg ctttcttaag acctggtttt ttacgttcga ccatacgtgc gtcacgagta
     6421 agtaagccag cacgtttaag agctaaacgg aagtcaggat caacttctag caatgcacga
     6481 gcgataccat gacggatagc tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca
     6541 aatacgtcat atgcgccttt tgtttcagta acagcgaaag gttgattgat tacttcacgc
     6601 aagtcagcat gtggaatgta ttcttcaaca tcttttttat ttacagtaat tttgccagtt
     6661 cctggtacta gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc gatatattga
     6721 acttgtgcca atgaaattcc ctcctattag attaagtttg tgatgtctaa aacttctggt
     6781 tgttgtgctg catgtggatg ttctgctcca ccgtatacgt tcaattttgt aaattgtttg
     6841 cggcctaaag tgtttttagg aagcatacct tttacagaag tttcgatcaa acgacgagaa
     6901 tttttagcac gcaattcgcc agcagtgatt gatttcaatc ctcctgggta gttgctgtga
     6961 cggtaataaa ttttgtcagt cgctttttta cctgttaatt tcactttgtc tgcattgatg
     7021 acgatgacga aatcgccagt atccaaatga ggtgtgaaag ttggtttatt tttaccacgt
     7081 agtacagatg caacaactgt tgaaagacgt cccaatggaa catcagttgc gtctactaca
     7141 taccatttac gttctacttc gcctggtttg gccatatatg ttgtacgcac gttattttcc
     7201 tccaatttaa gttctgatgt ttgacataca cttcgagttt ccggggctct ttgtggggca
     7261 aacaatacca tttaacatat tacccgctaa tctagtcaat gtcaacgttt ttctgcttga
     7321 ttgaaaaatt tatatgaaac tttacttctt ttctttcttt tatataaaag ggtcgtttcc
     7381 tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc ccttattttg
     7441 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca taaaacataa
     7501 gaagctgttt tttaaaattg tttcttgtga ctcggagctg aacggcaggc ttacaacctc
     7561 ttggtaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg
     7621 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
     7681 ctttttcggt caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat
     7741 gagcttggat catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt
     7801 gtgttggatc gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta
     7861 gataacgata gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc
     7921 caatttcatc agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac
     7981 cgtctaatgt gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga
     8041 actcatccat cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt
     8101 ggtgaacaac aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg
     8161 tttgttggaa tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag
     8221 actttcaatg aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt
     8281 tatccgtaaa gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg
     8341 ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata
     8401 ttttttatga tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc
     8461 ttcactgata ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc
     8521 ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg
     8581 acgaaaaatc ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc
     8641 ctggcaaact ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt
     8701 catcatttct ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc
     8761 gtcccaaagc ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc
     8821 cgctggcaca aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt
     8881 agcagattga acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc
     8941 attgctagtc gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc
     9001 catttctgga tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac
     9061 ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc
     9121 tgcccctccc aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga
     9181 tggtagacct gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat
     9241 gttcaagtag tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt
     9301 tcctgaagct gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc
     9361 tttgataccg acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca
     9421 cttgattttg ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc
     9481 tttgccaagc acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca
     9541 agtgatcatt cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc
     9601 agaggagaag gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt
     9661 tataacggtg cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt
     9721 ttcttggatc aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa
     9781 agcaactgct ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc
     9841 acctcaaatt ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa
     9901 agggggagat ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg
     9961 tttaaccgcc cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt
    10021 caaatgctgt cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca
    10081 acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc
    10141 cttatgtggt ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac
    10201 gttcatatgg atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt
    10261 ccattctcac acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg
    10321 cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta
    10381 tggtcaatcc tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt
    10441 gtctctagaa gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt
    10501 ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt
    10561 gagcagtgcg ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga
    10621 cttgtcacaa cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg
    10681 cgacaggttc acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct
    10741 caaaatgctg gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc
    10801 ttatttcttg acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata
    10861 aagattgtaa tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata
    10921 aaattcgctt cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg
    10981 caatgcttgt tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac
    11041 tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg
    11101 ctgccaatag atttccacca actgatcata agaaataact tctggatcat attcgatctc
    11161 tactgcttct gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac
    11221 atgcccgcca gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt
    11281 gaccatacac caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc
    11341 ttttttctca tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa
    11401 aacggaaact attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga
    11461 tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct
    11521 attcccaata catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca
    11581 atgaccaagc atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct
    11641 ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc
    11701 aaattcgttt tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg
    11761 atgtttgttt ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca
    11821 tccatttttt gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc
    11881 tctttttctg agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg
    11941 gttctggata ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg
    12001 ttagctaaaa ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct
    12061 aaatcgaata gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa
    12121 gtaaagatgc gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt
    12181 cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg
    12241 ctttttcgct tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta
    12301 ctcttctagc aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt
    12361 cgcctacgat tacgaggtct gcttgctgta cccgtttatc gaactccagt aaatccaata
    12421 cgtagtcgat gccagagact attttccctg aagcaaatgc taacagtcct gcacccattc
    12481 caccaccagc actactgccc gatgcctcga ttacttgagg ggcaaatctc atataaaaat
    12541 catgcatggc ttggtcagcc actgctaatt cattttcttt caagcctttt tgtggtccga
    12601 aaacaaaaga agcgccttct tttccgcata aaagattagt aacatcagta atgacagtga
    12661 ttttggcttt aatcagctca ataggcacgt tcgttttatc aactgtagca attttaccta
    12721 gattttctcc cagtgcttct actttttgat tatcttgatc gtaaaaagca taacctagac
    12781 cttctgccat gccgatgccg ccatcgttgg tactacttcc gccaacgccg atgatgatgt
    12841 gtcgaacacc ttgttgcaac aattgtcgta tcagttctcc tactcctcgc gtggataacc
    12901 taagtggctg ttgttggttt agtgggacat ctttcaatcc gacgatttct gccatttcaa
    12961 aaatcgcagt ttgaccgtct gaagcgtact ggatttcttt gtttttgcca aatgcattag
    13021 taacgactgc tgtttgccgc ttcaacctta aactgtctgc taatgatgaa accgttcctt
    13081 ctcctccatc gcctataggt agcagatcat attcagcctg aacaaaaact tcttgaaaac
    13141 ctgattgaat ggcttgtgct gcctctaagg ctgtcagact ttctttgaaa gaatctggcg
    13201 caatcagaat acgcattatt ttcccctccg ttccaagatc agttcttttt ccagctggta
    13261 ttggtctcca ttgacaaaaa taggtaatgt ttctttcgtc tcttttttta ttactatttg
    13321 tttttttgtc aaaacaaata gcaacctgtg atttttccaa ataaaaggaa aagaaatttt
    13381 ttcccatgct gaaggaagtc ttggagaaat agaaagagtt ccattcgtta ctgtaatacc
    13441 tgaaaaacca aatataactg ctagccatag tgctcctaat gaagcagcat gaattccgtc
    13501 atctgatgaa tgcggatctg gtcctaaatc aatcaggcac gcttcttgga ataaatgata
    13561 cgcccattcc atttcttgga tacggcaagc ctctatggcg tgaatcgctt tactcaacga
    13621 tgagtcgtgg attgttttgt tttcatagta atgcaaattc ttagaaacga ttggttgaga
    13681 aaaatactca ggtaataagt ataacagcat gacaagatct gcttgcttgc tgacttggag
    13741 ttcatttact tcgctacggg cataatcaag taaaatagac tggctgccag gacgttcttt
    13801 gtaagtggtc aaatcaataa ctggcttact gagatacgtg tcatcttgcg gaatcagctg
    13861 ttctttgtta ggcactggca aatataactt atctaaaaaa ttttgacatt ctttcaaaaa
    13921 aagcggatcg ccagcttgat atttaacagc taaggccaca ttataatgcg ccagatagtt
    13981 cgtatatgca ttattgtcga tatattctgt gtattcatct ggaccaatga ctctgtgtat
    14041 ctctaaatga tcatcgactt ttacagctct gctcagccaa aaagctgctg tttcatgaat
    14101 gagcgttttt ccctttgttt ctaaaaacga ttgatcctct gttgcttgga catactcttg
    14161 aacggcataa gcaaggtctg cactgatatg atgttctgcg gcagctgaag caacttcttg
    14221 tcttgttccg gttttgatat tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg
    14281 tgcgctttcc catgggaaaa gtgcaccttg atagtgataa tctcttgctt tttgccgtgc
    14341 gccttctaat cgatcataac ggtacagcag caaattcttt gctacttcag gaaactgata
    14401 taaaaagaaa ggcaggataa aaatttccgt atcccagaaa acatgccctt tataaccttc
    14461 gccagttagt cccttcgccc ctacactaac acttggatca ttgcggggag tcataatctg
    14521 taaatggtaa gcagcgaagt ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt
    14581 tatccgatgc tgatgccaaa aatctgtcca ataagacatc gactgctcta aaatttctgt
    14641 ataactaacg cttttctggg cttgggcaat acttttctta aacgggtctg tttgctgaag
    14701 agaggtagag agcacgatga tcttctccag cgtaaaggac gtattttttc tgatcttttc
    14761 tgaaaaggaa gcggtgatcc ggcgattttt tgcttggaat acacctgctt tattgaattt
    14821 agctgcaatc ccaatatcaa ttttactttc tgttgtctgg taggatgcac tgatcaaatc
    14881 gtcaccaaat actcgcagtt ctttttcaac caactgctgt gtaccaaaat tggtttgctg
    14941 tccatcgata cccgttttca catctacttg gcagtcttcg gtcaacggct tgatgaccac
    15001 tttcagggct gcctgatgga gattattttg atcagcaaca cgacaagtaa tgacttccac
    15061 tttttttcct tgagcagttt caatgagcgc atagcgttta agctctccca tttctaaatc
    15121 tagtgcccgc tcgtatgcaa gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat
    15181 ttttatttcc catctgatga catctgggag attcactaac tcggctgatt ctgctcctaa
    15241 aggtcggtta tatacaccag ctgcaaacat tccccttact tgattccgat agtcttcctc
    15301 ctggcttcct ctgataccaa aaaatccatt tccaatggtc atctgactcg catacttatt
    15361 gatatactct aaatcaaact gtttttcttc tactttcata aggtaataac ttctcctgtt
    15421 tctgccgaac gatataaatt ttccaccaga ttctggatga catatccttg ccagccatct
    15481 gcaatcatct gagtctggtc accgagcgcc cgattcacaa aatgagccat acttagttca
    15541 tgtgggtcat taactggcgt ggtcttttgt aaggaaacca gctttccatc tacatcttcg
    15601 aatagttcgc ctggatacag attagcacct gcctgatcgc cacaaaaagc cacattcagt
    15661 ttgtcttctc tgatattcaa agcaaacgaa gtattcagtt ggatgatctt gccgttttct
    15721 aattccagtg tgccaaaaat tgcatcttct actgtgaatt tgcttggatc ccatttcccg
    15781 aatttccctg aatttttttt cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt
    15841 actttaggaa atccagtaat atacatcgct gtatcaagca tgtgaatgcc ataatcgatc
    15901 aaaggcccgc ctccttgaag ttctttgtcg ataaacgttc cccagcctgg aacaccagac
    15961 cgacgaagcg cattcgcttc tacgtaataa atatccccga gaacatcaag atgattcttg
    16021 atcattgttg cttcgtcaga aaaacgatgc tggaatccat aagccaaaat ctgcccgttt
    16081 ttatcggctg tctctgccat ctttcttgct tgatcagctg tcatagctgg tggtttctcg
    16141 cacatcactg aagcattagc ttcgagggca gccaatacat gttcgaagtg aaagcgattg
    16201 ggtgtacaga cactcacgat atcgggagaa atctcattca gcatttgtgt aactgatgta
    16261 tacgcatgag aaaatcctgt acgtcggcaa aagtcttctg ctcgctggct gactggatca
    16321 accacagcgg ccaattctaa gacatctgat cttttttgat aataagcagc atgtactttt
    16381 tctgctactt gtccagcgcc tatgattgct actttcttca tttatttctc cctcctttgc
    16441 ttatttactt attgttattc tttctctatt ctttctctaa atcatttaaa tgacgcgctg
    16501 tttccagaca gtttttcaag tattgcaatg attcctgata aattttcact tcattttctc
    16561 ctcgcaaacg acattcatat accatataac cttcatactc attttgaagt agcgtttgaa
    16621 aatggcgagt aaaatcaatt gcgccagatc ctggttggta gcgatgatta tctgctaaat
    16681 ggatatgacc cacatactct ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa
    16741 tattcatatg ataaaaatca gcgatgatct gcacatgttt caattgatta gagtcaatgt
    16801 aatgacgcgc atccgctaac aaattgatca tatggtcttg gtatcggttc aaaggctcca
    16861 aataaataac cgtacctgtt tcttcggcta ctttatctaa ataacgaagt gattcgctga
    16921 ctgctttata gtctccttct ttgcttcggg gtgaaaccat cggcggtaat cgataagtga
    16981 acattcccca agctgctggg ataacgattc ctttgcctcc aacctcgctt agtgcacgca
    17041 aaatctcagc aatttgacgt acaccattaa gacgccgttc ttcgataaag tcaccgatcc
    17101 acccatcgta gccgttacaa gctgtcacta ctgggatccc tgtttctttg attgcctgtt
    17161 tgactgtgtc gatattttct attaacaact taccgtcgat ttcgtattct tcaaatccac
    17221 agtctttgat aaatctaaat ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg
    17281 taccgaattt cattgttctc ctcctcggac ttgaacgccc attttgatac tttcttctgg
    17341 gtgctggtct acataacgca tgtaagcttc tgcgctttct gcaaatggca caactggatc
    17401 aatgatttct tcacaatcca agtaaccgtt catcaataat tcccaacatg tgtcctcgat
    17461 tcgtttacgg ttccatcttg ggtaatctgg gtttggttca ctagatgctc gagcaaagac
    17521 gatttttgca ttattgaaat gtgcttcgcg accaagatta aacccagctg gaaaaggttt
    17581 agcaaaggca acataagaaa tgatcccgcc atagccgatc cctcttaaag aagcttgtaa
    17641 tgcggcagca ctgccactcg tttcaataac tgaatctact ccacgtttat ctgtcaattc
    17701 ctttagacga aaaccgacat ctgctttcat aggatctagc gtgtaatcgg caccgtattt
    17761 ttctgctaat tctctacgct gcgctatggg atcgatccca acgatggtag tagcacctgc
    17821 ttttttagct aattgaatcg caatttgccc gatagcgcct aaaccgaaga ttgctacagc
    17881 atcacctgct cgaacatgtg cttcccggat accacctaaa gcgaattgag caggatcgta
    17941 acatactgca tttttccatt gatccaaccg atcaagttta cgtagtttat aattatcaac
    18001 accgtttgta atcagcgttt cacgaatggg tccataagtg cagactaact ctcctttttg
    18061 atactctgtc accaaactgc ccgtttcttc gatcgtaccg acaatcatat ttcctaattg
    18121 aaattcgcca aataccacac cgcgttggct tccttcttct cttggcaaga ataggcgcca
    18181 ttcttctgaa aattcttcat cgataaatgg acttaatcct ctaaagtcga caatttctgt
    18241 tcctggtttt ggagtagcga aaattactcg gatacgtact tcgttctttt cgatctcacg
    18301 gtcttcgtaa tctttgaatc cggctattct tggttctaat gcaactaatt ttttcatctt
    18361 ttctttcctc tttcttttct ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa
    18421 taaatcgttc agataacgca tacataatga tgactggtaa agcggtcacc aatgaagcgg
    18481 ccatcattcg tccccaaatg tagtctggtg tactgaataa tgtgttcaaa ccgattggta
    18541 aagtaaattt ttcggaggtc gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa
    18601 ccataaagca ataaacgaag acagaaatga ttccagaaat agccaatggt ataatgatcc
    18661 gaataatgat ctggatccgg ctaagaccat cgatcattgc tgcttcttca atatcagtcg
    18721 gaatcgtgtc aaaataactt tttagcatga atatcgcagt aggaagtgtt tgaacgacca
    18781 tcgtgataat cagtgctgta ttggtatcat acaaacctag accggaaatg attttaaata
    18841 atggaacaac gatcaaaata ccggaaaaca tataaacggt gtaaaagcta gcattgattt
    18901 tcgagcgagc tttgaagcgt agttttgaca aagcgtatgc cccgctgatc ccaatcacta
    18961 ctgcagttac ggaggctatc aaggagatca ctaaactatt tttgaaatac atgatgaatg
    19021 gaaaaatctc agcattgaaa atatcctgat aatgttcaaa agtccattct ttcggaaaaa
    19081 tagtttgtgt cgttgcaatt gcttcttgtg aatccttgaa tgatgtcatc agcatgatca
    19141 aaaatggaaa caaattgaca gctaatagca acaaaatcgt cgcgtaaaat aagattttcc
    19201 tgctcttttt atttttcatt tcaagtccac cttctttcgt gagagcaaga cgaccgctgc
    19261 aataatgatg aacaatagaa tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa
    19321 ggctgtctga tacaaataaa cgcctaagat attgacttta ttcgtcagta gatagacatc
    19381 tgtaaacata tagaacatcc agatagatcg taaaacaatc acagttgcta gtactggttt
    19441 gattgccggc agtgtcacga tcttgaactt ctgccaagca tttgcaccgt ccatttcagc
    19501 tgcttcataa agcgttttgt cgactgtttg caaaatagcc aaaaatgaaa taaacgcata
    19561 cggatagtat ctccagatag caaacaaaac gaccataaag aagctagcaa ctggctggtc
    19621 gaaccacagc ggtgcttcgt tgaaaagctg caacctatct actaacagat cattgacgat
    19681 tccataacta ttattgaaca tatatttcca agcaaaaatc aaagaaatag atggcgtaac
    19741 ataacttaat aaaatcagtg aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa
    19801 aatagctacg gctaatccta aagctgtact gcctgctaca actaagaatg tgtaagcaac
    19861 tgttaaacct aaagaacgat aaaattcact atcacttaaa atttcaagat aattgcttaa
    19921 tccgataaaa acggcgtctg agttaggatt cagcggcata tcgaaaaaac tgatctgaat
    19981 attggaaagc attggaatca gcactaaaac agctaataaa agcatgcttg gtgctaaaag
    20041 tcccatggct aattttaagt cagaatgttt ccttggacga ttcattgaaa cctccccctg
    20101 ataatctgcc aatagctatc tacttttttc gaacaccatt ggcagaactt cttgttattt
    20161 cgtatttttt tgtgcgtcag ctaaagcttg atcgacatct tcgtttccga cagttacttg
    20221 gttcactgct tttccgatca cacctgagct tgtgatatct cccattttcg tgaaattctt
    20281 ttccccgact agtccaaaga cttgtacttc atcaaaagca ctagcaatct cagatggcag
    20341 ctcgccaaaa gacttgatga cttcattttc tttgtaacca tccagttcga ctacttgttt
    20401 attcactggt tgagcaccac caggagacat caagacccac ttttccatgt ttttcggttg
    20461 agataagaaa gctgtgaatg cttcagcagc ctctttttgt ttctcgtcta gaccagaaga
    20521 aatcgtcaat ccagatactg tgccatagac agcttgatct ttttgtgttg ggatagcaaa
    20581 accgatattt tctgagttcc ccgcttcata aacagaaggc aaaatgtaag ttgaatagat
    20641 tgccattggt gctgttccat tcatgaaagc atctttgatt tctgtcacat cattagagcc
    20701 tggcatcgtg tattgggata agttttgata aaacgacaat gcttctctca tcttttccgt
    20761 atcgatcgtt acctcgcctt catcatccaa aacatttgct ccatttgaca aagcaaattg
    20821 agagaacgct tgctcactca ttgttccttc agcagtaggc atagcaattc catattgttt
    20881 attctcttta tctgtgaaat gttgagcaat ttttaaaata tcatcccaat tttctggttc
    20941 tgaaaaacca gcttctgata atttttcttt gtcataccaa ataccttgaa cccagccgct
    21001 aattggtgca gcaatatagc tttttccatc ttctgaacga acaaggtttt tggctccgtc
    21061 ataataatta tcttctccag cttcctcgat aacgttttgg actgccgtct gatcaatcag
    21121 ttcatcttta tccatcactt tagcaaaatc ctgactgact tccattactt ctggtaattt
    21181 accggaacga gctaacgtaa cgatttttgt attgaacgct gattcttcta ccggcacttg
    21241 cttgattttg atgtctggat tttctttttc aaaatcagca accagttcat tgatcacact
    21301 taatcgttcc tgttcgacag aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt
    21361 agagctttga ctttcaccac aagcagtaag aagaaatcca gtagctagca gacaggtagt
    21421 aacaataatt tttttcattt ttttccacct cttattttat tattttcagc cacatatatt
    21481 catacggctg gaatatcaac tttcgttctt gactgcaatg gataatttgt ccagaaaaca
    21541 ggtctaggta atctcctttt ggtaaaagat aacctgctga ttgattggaa aaattatgga
    21601 taactaagac tttctcttcc ttcgcttcgc gaataaaact gataaaagaa tctgctgagt
    21661 ccagtaagta aatcaaacta ttcggatgga aggctttttc tttttttcga atagatagca
    21721 tctgggtcaa ttcaccatat actgctgttc ttaatggatc tgtctgcaac tctttctcaa
    21781 tatctgctaa caaaaatttt tgccgattga tttttcgatt ctctcctgtt ttttctacgc
    21841 ctttgtaatc atttcgactt cccagcacac tttgaatata aattgccggc acgcctggta
    21901 acgttaccaa aaatgaatga gcagctaaaa agcgtttgct tctccattcc gtcgtatctg
    21961 ttggagaact taacgcatct agatacgtag cgtttatctc atatggactg gtcgttccat
    22021 ctgggttttt cttgtagttt actcgggtac cctctttctt cagctgttcc actaattcaa
    22081 ggatcgtttt ttccggcaga atacctcgta ccggattcaa tccgataccg tcatgggagg
    22141 ctaaaaaatt aaaaaaagtt gtttttttcg atgcgaaatc gatggttttt aaccattctt
    22201 tcaacttact tgtattaccc gaacgaatcg cttgaagggt caaaggtggt agcgaaaact
    22261 gatagaccat ctgtgcttca ttctcgccat tcccaaaata actgatatta tctttgtgtg
    22321 gcacatttgt ctcagtgatc aataccgttc ctggagctgc ttcatcagcg attgcacgga
    22381 ataattggat gattttatgc gtttctggca ggtgtataca agaagttcct agttctttcc
    22441 ataaaaatcc cacagcgtcc aagcgaataa atgaagcgcc tttttctaag taaaacaaaa
    22501 gaatatcgat cattttcaat aaaacttctg gatttttaaa attcaagtct acttgatctt
    22561 cgctaaaagt cgtccatacg tgtttttctt tcccgttttt gtcaatgaac gttgtaagta
    22621 acgggctggt ccttggtcta gtcacgctgc ttgtatctgt atccagtggc aaatcaataa
    22681 aaaaatcttt gtacgtatca ttcaagttta aatattcctg aaaccaagta ctttcggcag
    22741 acatatgatt gcagacaaag tcgaacatta accgtgtttc gcttctcatt tcttctatat
    22801 cttcccatgt gcccaaacgg tcgttgattg cctgataatt tacaacggag aaaccatcat
    22861 cagatgtata tgggaaaaat ggcaagaaat gtacaactct aaatgtatct gccaaatact
    22921 tttgataaaa tttcttaaat gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc
    22981 cataagtaat caagaaaata tcttcttcgt cccaatgcag tttgcctgcc tggcctttct
    23041 tctgctgata aacactgact ttttgttcaa tcatagaggc ggttttctca gacaaatctg
    23101 agtcgcgata aatcgcttgt aaaagttttg tatacatcgg attccccttt cttttttggg
    23161 agcgttccca acgatttgat catattatgg aaaacgctta ctgtcaatag cattttttta
    23221 aataattttg atatacacaa aaatacaaat aagaaagctt atgtatcaat aattaatagc
    23281 ttcttaaaaa aatatggtct ctcatgatca tgcaagctat tcctaaattt tcaaaatcat
    23341 agacaaaaaa gaatgtctat tgtactatgg aattattcta aaacgctccc aaaaaggagg
    23401 tgcatataaa attgaaacct actatttatg acattgcgaa aaaagctaat gtctcaaaat
    23461 caactgtctc cagagtatta aataaccagc caaatatctc tgataaagca aaagcaagtg
    23521 tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga ggactgtcag
    23581 gaaatggttt tgacgcgatt ctagcattat ctcatcgtac ttctcatacg acctatggca
    23641 atcctttttt ttcagatgtt attcaatcca tttctgccgt ttctgaacaa aataacttcg
    23701 acttgattct acaaacggca aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga
    23761 tccaagaaaa aatgatccga ggaattatta ttttaagctc tacgatggat gaaacacttc
    23821 tttttgaatt ggatcaatac aatattccta ttgtggtaat cggaaaagtc ggaaacgact
    23881 acacgaacat ctactcagtg gacacggata atttccagga tagttacgat ttggtggaaa
    23941 cactgatcaa gaatggacac cgtcatatcg gctgtctcca tgctccgaca gaagtttatg
    24001 tatccaatga tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac ctagatatcc
    24061 gtaatgaatg gattatagac ggcggctttc gcttagaaga tagcatggca gcaatgaatg
    24121 atttaatgaa gctacgagaa agaccaactg cgattttttc taccgatgca ttaaaatcat
    24181 tgagtattta caaagtggca gaacaatctg gaaagcagat acctgatgat ctttcgatcg
    24241 caggattcag tgatccctta ttgtcctccc tattttcgcc acagttgact cggatagaca
    24301 ttccgacaaa tcagttagga atcaaagcca cagaactttt attccaacta atccaccacc
    24361 aataccctga agaaaaaaga atcattcttt caaccgagat tactctcaca gattcgatcc
    24421 aacaacaaga aatgtagaac agaattataa aagaacagaa acactgagga taagtcagtt
    24481 actctgttct tttttcaatc actcatccct cgttaccccc tcccggtatt ttagcgaatt
    24541 tttcttattt tccatctgtt ttagatagct gtggataact ataatgtttg tgttaaacta
    24601 ttttttccac agggaagaaa aaactatttt taaccacgtt gtgtataagt ttttatagtt
    24661 cgttttttta aacacattta cccttatttc aatccatgtt atttggggat acttatctct
    24721 aaaacataaa tagaataaca gacatgtcta ctggttttgt tttattcgag gaattattca
    24781 ataatagcta gcaaaatatt aaaaataacc gctctatctt cccatcaatt tattttgcat
    24841 tcaaaaaatg atctatcttt acttttgttg attgacttct cttggaagat gatcgttccg
    24901 ataccttaat tggatacgaa ccgatgttgt taagatcctt tcccactttt cttctagtta
    24961 gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt
    25021 ttttaaaata tctattttgt ttgattgttc attgcgtctt ctgcagccat ttgcgcttca
    25081 atatctgaaa tactgtagac attttcgctt gctacaccga cttcttttgg ttccatgtca
    25141 cgataacgtt gcataccagt accagctggg atgatcttac ctataataac attttctttc
    25201 aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt
    25261 tcttggaatg atgcagcaga taagaaacta tttgtttcta atgatgcttt tgtgatacct
    25321 agtaagacag gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt gtagttttga
    25381 tctttaaagt ctgcgatgtc taataatgta cctggtaaga tatctgtatc acctggatcc
    25441 attacacgaa ctttacgcag catttgacga accattactt cgatatgctt gtcgccgatt
    25501 tctacccctt gcatacggta aacacgttgt acttcacgaa gcaagtagtt ttcaacagat
    25561 aatacatcgc gaacttgcaa caattgtttt ggatcgattg atccttctgt caatggtgca
    25621 ccacggtgga tcatatcgcc ttctgctact ttcatacgtg ctgtataagg aacagtatac
    25681 gtacgtgtat ctgtttttcc tttgattgtt acttctttgg tacgtgttgc aggatcttct
    25741 gagatatcga tgacatcccc tgttacttct gtgatcactg cttgcccttt cggattacgt
    25801 gcttcaaaga tttcttggac acgaggaaga ccttgagtga tatcgtctcc ggcaacccca
    25861 cctgtatgga atgtacgcat cgtcaactga gtaccaggtt caccgattga ttgagcggcg
    25921 atcgtaccaa ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa
    25981 caatgtttgc agacaccatg ttttgtatta catgtgaaga cagaacggat tgtcactgtt
    26041 tcgacacctg catcaacgat ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca
    26101 atgatcaatt catttgtttc aggatgacgt acttctttac gggtgtaacg tcccactaaa
    26161 cgatcttcta atgattcgat gatttcattt ccctcgcgga tggcttggat ttcaagacct
    26221 cggtcagtac cgcaatcttc ttcacggatg attacatctt gggccacgtc aaccagacga
    26281 cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat cggtcatccc tttacgagcc
    26341 ccgtgagttg agataaacat ttccaagacg gatagtcctt cacggaagtt cgaaatgatt
    26401 ggcaattcca tgattcgacc attaggagcg gccatcaaac cacgcattcc ggcaagctgt
    26461 gtaaagttgg aaatgttacc acgagctcca gaatcagaca tcatgaagat tggatttttc
    26521 gcttccaaac cttccatcag tttttgttgg atgctgtctt ttgcagcatt ccaaacagca
    26581 atgacacgtt cataacgttc gtcatctgtg atcagaccac gacggaattg ttttgtaata
    26641 gtttcaactt gtttatgggc attttcaatg atttcttgtt tttcattcag tacactgatg
    26701 tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa gtctttcata
    26761 cggtcaagca tcttagaagt ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg
    26821 tttcctaagt ttttcttctt gaacggtaat actaattctt gctctttgat atgtgcagga
    26881 atatcagtac cagcttcaac aaagtatttg tccggtgttt ggacggtcaa gttgaagtct
    26941 gttggttcat tcagataagg gaattcagga ggcatgatct cgttgaagat gatcttacca
    27001 actgttgtga tcatcgtacg ttctttttgc cattcagtaa atggtttttc acctaatgag
    27061 ttagggttca caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg
    27121 gcttcgtttt gatctctgaa gaccattcct tcgccttcgc gtccttcttc ttccattgtc
    27181 aagtagtagt tacctagaac catatcttgg gaaggagtaa caactggttt accatcttta
    27241 gggttcaaga tgttttgagc agctaacatc aacatacgtg cttctgcttg tgcttcttca
    27301 ttcaatggaa cgtggacagc catttggtct ccatcgaaat cggcattata agcttcacat
    27361 acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata
    27421 ccaagtcggt gaagcgtagg tgcccggttc agcaatactg ggtgttcctt gattacgtct
    27481 tctaaaacat cccatacttc gtcttcttga cgttcgattt tacgtttcgc atttttgatg
    27541 tttgaagcta gttcgcgttg aacaagctca cgcatcacga atggtttgaa taattcaatg
    27601 gccatttctt ttggaagtcc acattggtac atcttcaaga aaggtccaac aacgataacg
    27661 gaacgacctg agtagtcgac acgtttacct agcaagtttt gacggaaacg accttgtttc
    27721 cctttcaaca tatgagaaag agatttcaat gggcggttac ctggtccagt aactggacgt
    27781 ccgcgacgac cgttatcgat caatgcatct actgcttctt gcagcatacg tttttcattt
    27841 tgaacgatga ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt attacggttg
    27901 attacacgac ggtaaagatc attcaagtca cttgttgcaa aacgtccacc ttctaattga
    27961 accattgggc gtaaatctgg tggaataaca gggataacgt ccataaccat ccagcttggt
    28021 tgatttcctg aagcgcggaa tgcttctaaa atatccaaac ggcggattgc acgtgtacgt
    28081 ttttgtcctt gtgcagattt caattcttct ttcaattcgg ctgcttctgc atctagatca
    28141 acattatcaa gtaattgttt aatcgcttct gcacccatag cagcttggaa ttcttgtcca
    28201 tattgttcac gtttttcgcg atattcgcgt tctgtcaaca attgtttttt ctctaaattc
    28261 gtatttcctg gttcgatgac tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt
    28321 gggctcatgt ctaatacaag acccatacgt gaaggaatcc ctttaaagta ccagatatgt
    28381 gaaacaggag ctgctaattc aatatgtccc atgcgttcac ggcgaacttt cgaacgagta
    28441 acttccactc cgcatcgatc acaaacgatt cctttataac ggatacgttt gtattttccg
    28501 caggcacatt cccagtcttt tgtaggacca aaaatccgct cacagaacaa cccttcgcgt
    28561 tcaggtttta aggtacgata gttaatcgtc tcaggttttt ttacttcacc gtaagaccag
    28621 cttctgattt tttcgggaga agccaaacct atttgcatac tttcgaattt atttacatcg
    28681 atcaaaaggg gttccctcca tttcatttaa tggcaaaccg caagacggct gatcttcgac
    28741 cgccttgcag ctcatagacc ggattagtct aaattatctt ctgcggtttc aaagttttgg
    28801 atgacatcgt ctttttcatc ttccacttgt tcagcagctt tcttttctaa ttctttggca
    28861 gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg
    28921 tccatgtcgc gaagttcgat ctctgtttct tcgatatcca agacacgcat gtccagacct
    28981 agtgattgca attctttcac caatacgcgg aatgattccg gtacacctgg ttttggaatt
    29041 ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg
    29101 tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag cttccagtgc ccatacttcc
    29161 atttcaccaa aacgttgtcc accaaattga gctttacctc ccaatggttg ttgtgtaacc
    29221 aatgagtaag gtccgattga acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg
    29281 atcatgtaca tcacaccgac agacacacgt ccatcaaatg gttcacctgt acgtccgtcg
    29341 tacagaacag ttttcgcatc gctggccata ccggcttcac gaactgtttc ccatacgtct
    29401 tcatcacttg caccatcaaa tactggcgtt gctacgtgga tacctaactg acgtgcagcc
    29461 atacccaagt gtaattccaa tacttgcccg atgttcatac gtgaaggcac ccctaaagga
    29521 ttcagcatga tatcgattgg tgttccatca ggtaggaatg gcatatcttc ttccggcata
    29581 atacgggata caaccccttt atttccgtga cgaccggcca ttttatcccc ttcatggatt
    29641 ttacgttttt gaacgatata aacacgaact aacatgttta cgcctggtga caattcatct
    29701 ccagcttcac gagtaaagat tttcacatcg tgaacgatac cgccgccgcc atgtggtaca
    29761 cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa agatcgcatg caatagacgt
    29821 tcttctgcag ataattctgt tacccctttt ggcgtgactt tccctacaag caaatcgcca
    29881 tcttggactt ctgcaccaat acggatgata cccatttcgt ctaaatcttt taatgcgtct
    29941 tccccgacat tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct
    30001 tctgattcat attcttcaat atggatagaa gtgtatacgt catcttttac caaacgacgg
    30061 ctcatgatga tcgcatcttc atagttgtac ccttcccaag tcatgaaacc aactaaaacg
    30121 ttttggccta gagccatttc gccttgttcc atagatggtc cgtctgccag agtatcccct
    30181 ttttcgactt tctcacccaa atgaacgatt ggacgttggt tgtagcttgt tccggagttt
    30241 gaacgacgga attttgttac atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg
    30301 atttcagaag catcaacgta ttcaacgaca ccgtcatgtt tacataacaa agcagctcct
    30361 gagtcatgcg ctgatttgta ttccatacca gtaccaaccc atggtgcttg aggattgatc
    30421 aacggcactg cttgacgctg catgttggca cccatcaagg cacggttgga gtcatcgttt
    30481 tccaagaaag gaatacatgc tgtcgcaacg gcaactactt gtttaggtga tacgtccatg
    30541 tagtctactt tatcaataga tacttctaag ttttcacttt gagcacgagc cataacaacg
    30601 tcttgagcaa atgttccatc ttcattcaat gggctgtttg cttgggctac gatgtaatgg
    30661 tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct
    30721 acacgacgat aaggtgtttc gataaatccg aatttattta ctttcgcata actggacaaa
    30781 ctattgatca acccgctgtt tgggccttca ggagtttcga tcggacacat acggccatag
    30841 tgggagtagt gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa tccgccaggt
    30901 cctaaggctg aaagacgacg tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg
    30961 aactgtgaca actgagaaga accaaagaat tctttgatac ttgccacaac tggacggata
    31021 ttgatcaatt gttgaggtgt caatgtttca gtatcttgga tcgacattct ttcacgaacc
    31081 acacgttcca tacgtgctag cccgatacgg aattggtttt gtaaaagttc accgactgaa
    31141 cggatacgac gatttcctaa gtggtcgata tcatcgacat tgccgatacc ttccatcaag
    31201 ttcaagaaat agctcattga agcaatgatg tcggctggac gaaccgtttt tacagctgct
    31261 tctggatagc cgtttccgat cacgtttact tcacgttcag ggtctcttgg tgaaaatact
    31321 ttgatgactt ggacagtcat tggatctgtg accacaccgt cttctgatgg atagtaagtc
    31381 actgaattta ggccattatc aatgaaagga gctagtgttt ccatgacttg gtgagtcaga
    31441 actgttcctt tttcaacgat gatctcgcct gtttctggat caactaatgt ttcagctaat
    31501 gtcagattca atagacgtgt tttcaaatct aattttttgt ttactttgta acgaccaacg
    31561 tttgctaagt cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt acgtgagctg
    31621 tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct
    31681 gttcttgagt cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg taatgtttcg
    31741 ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa atcctaacgc acgaactaat
    31801 acggttaaag gaatcttacg tgtacggtcg atacgtacat aagagatgtc ttttgcatca
    31861 gtttccattt ctagccaagc tccacggtta gggataacag tagaaccgaa tccttctttt
    31921 ccgtttttgt cgacttttcc atggaaatag acacctggtg aacggactaa ctgagaaacg
    31981 attactcgct ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa tgggaaatca
    32041 ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt
    32101 acatgtaatg gtgcagagta gtttgcatca tgcgcacgag cttctgctac tgtatacttt
    32161 ggttctttca attcataatc tacaaattcc aaagatagat tgccattgaa gtcatcgatt
    32221 ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta agaaccattg gtaagagtct
    32281 gtttgaattt cgatcaaatt tggtaattcc aacacttcac tgattcttgc gaaacttcta
    32341 cgttcgcgat gttttccgta ttttactacg tgtccagcca agctcttcac ccctctaaaa
    32401 tgtcattcaa aaagactacc cgatcaattg ttacaataat atttcaaata ttatttttag
    32461 gtaacaacag acattttttg cggcttttag gcaaaaaaaa accaaaaata gaacattctc
    32521 ttgaaacctt acttcgtgag cttctacttt tgtttcctta tattaaagcc gaaacggaca
    32581 atatttcgtt tctgcctttt cttcatcaga tagtttttgc aacttcatat actacactta
    32641 ttttctataa aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac attttcttaa
    32701 tgttttcaat tttatgtttt caattatctg acaattctta attgacagac ctttttatct
    32761 cggttatact ttagaaaatt cattttgtgg aggaacttct atgcgaacca acatcaacca
    32821 aatcgcacca acacaactga ataactttta ctttagctac tttagatagg tttgtattca
    32881 tcatgatgga tacaaacgtt aaacgacagt ttgtatctat gatggctaaa gaactttgta
    32941 ttgcagcaaa aagccacgta gacaaaatgc taagtggctg agcagtgaaa atacaaagta
    33001 aaagtatcaa gcattctact ttttcaattt attttccgac ttttcaatcc atttggacat
    33061 tttgtctgaa tggatttttt gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat
    33121 ataatcatcg gattaggcgc tgccctgcta ctagctttag gaggttgcgc acaaaaagac
    33181 aattccgcag aggcatcaga tggacaaaaa acaattggcg tattgcaact tgtacaacat
    33241 aactcgttag atgctgccta taaaggattc aaagaaggat tagcagaaga agggtataaa
    33301 gaaggcgata atctaacaat cgattatcaa aatgcccaaa acaatcaaga caacctgaaa
    33361 agtatgagtg agaaactcgt caaagaagat cctgatttgc tacttggaat cgccactcca
    33421 gcagcacaat ctttattaaa tgaaacaacc actatcccga tgactgtcac agcggtcaca
    33481 gacttggaac aagcccattt agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc
    33541 acgacagata tggtgccaat cgaaaaacaa atcggcttac tgttaaatat cgtgccagac
    33601 gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa actctaagat ccaagctgat
    33661 ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca aagtactgac agcaaattcc
    33721 acaaatgatg tccaacaagt gacgaaaagt ctagctaaag atgtagatgg aatctatatt
    33781 cctactgata acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca
    33841 aaaacacctg tcgttgctgg cgcagttgat caagtcaaag acggcggtct agcaacagta
    33901 ggaatcgact acgaaagctt gggtaaacaa acagggatca tggcagcaaa aatcttagac
    33961 ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg atttggaact gtatgtcaat
    34021 aaagacatgg ccaaagcatt aggcatcaat ccagacagca tcaaagaagc tgaataaaac
    34081 acaggaggat tctcatgaca aatttgctct ctcttttctt atcatctgca tcgcaagggg
    34141 tcttatgggc cctacttgcg attggtgtat acgtcacttt ccgtatcttg gacattgccg
    34201 acttgactgc tgaaggaagc tttcctttag gtgcagggat cgcggcagta agtattacaa
    34261 atggctatca tccattgaca gcttgtcttc taggcttttt agggggtgcg gcagcagggc
    34321 ttgtttccgg actgcttcac acaaaattga aaattcccgc ccttcttgca gggatcatca
    34381 ccatgactgg tttatattct gtcacatccc gtgtgatggg tgcgccgaac atctctttat
    34441 tgggtcagcc aacgatcttt cattgggtcg agtcattcgg cataacaaaa gacaacgctg
    34501 tactgatcgt cggtctgctt tttgctttga tcgttgtatg tttacttgtt ttgttcttac
    34561 gtacagaaac aggacttgct attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa
    34621 acgggatcaa cacagataat atgaagatca tcggttatat gatttctaat ggatgcattg
    34681 ctctttctgg ctccttgctt gcgcaaaaca acggctttgc tgatttaaat tctggtattg
    34741 gaacgatcgt aattggactt gcttctatta tcatcgcaga agttttgtta cgtaaccagc
    34801 cactatttct ccgtctcttg actgtcgttt taggtgcggt catttatcgt tttattttag
    34861 cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc
    34921 tagtgatctg tctttctttc ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga
    34981 aaggcggtag tgtcaaatga atgacgtctt aacattggaa aatctccatc atacctttga
    35041 aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt aatctttcct tagatcccgg
    35101 cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa tctactttgc tgaacagcat
    35161 cgccggtaca ttttctgtcg atcagggtca aattttattg aatggaaaaa atatcacgaa
    35221 aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc ctaaactagg
    35281 gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa
    35341 acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag accagctggt
    35401 tcggctaaat ttggggttgg aaaatcgttt atcctcagaa atcggtttgc tgtctggagg
    35461 acaacgtcaa gctattacgt tattgatggc cacattacag cgtccagaac tgatcttatt
    35521 agatgaacac actgcagcgc ttgaccctgc tacttccaaa acggtgatgg aattgacaga
    35581 aaagctgatc actgagcaga atctcactgc ttttatggtc actcatgaca tggaagacgc
    35641 gatccgctat gggagtcgac tgatcatgct gcatcaagga aggatcgtcg tagatatcca
    35701 gcaagaggaa aaacagcagc tcacagtgcc ccaattatta gcgttgttca aacaaaacag
    35761 cggcacggca ttaaaagacg atcaagtact tttatcttca taatcagaaa gaggatggga
    35821 caaaaaatga acgcccaccc tcttcttttt tctctttcct tctttcgttt ttgctttatg
    35881 tatcaaccgt tgtttcttcg aaaactattt tgttctctcc ttcttcctga tagaatggat
    35941 gggaaaggag cttgatgcca tgacaacgaa agacaaggta cttcaattat tgaaaaagac
    36001 accagatttt ctttccggag aaaagttggc acagcaactg gaagtttccc ggacaagtat
    36061 ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt tttgagcacg ggcctacagg
    36121 gtatcgttac ctgccctctg acgtactaga tgcaaaagaa atccagcaag agctacggtc
    36181 gtttattcct gatttgaaag tacaaacgat gttgacttct gaatctacga tgaaagatgt
    36241 caaactggca gcagctcaag gagattctgt ttcgaagtta tttgttgctg atacacaaga
    36301 acaagctcgt ggacgttttg gacggccttt tttcgctgaa cctggacgag gaatctacat
    36361 gagtctcttg ctccatccag acaaacggtt tgatgaattg cctcaatata cgatcatcgc
    36421 agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg
    36481 ggtcaatgat atctacatgg atggaaaaaa agtctgtggg attttatctg aagcagtcag
    36541 tgatatggaa agcggcagga tcagtcatat catcattggg atcgggctta atttttctgt
    36601 tcctcaaaat cattttccag ctgatctcca agaaaaagcc atctctgtct ttcccgacgg
    36661 acaagctagt gtcacaagga atcagctaat cagcaaaatc tggcaagaat tgttcactta
    36721 tctttctcac ccagaaaaag cgttggactt ctataaagaa aaatcattcg tattgggaaa
    36781 aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga
    36841 tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg agtttgtctt ctggcgaaat
    36901 cagtctcaca tctatccgta cgcaataatt atcagatttc acaaaaggac acctgatctc
    36961 atacagtgtc cttttttctg aattctcttc aaaagactct ccttattcta tcagctgaca
    37021 gtttctctat cctctagaaa aaggtattct aaaaatatct ataaagcatg gtacactttc
    37081 tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca aaagagatag
    37141 caggccgctt cctttttcaa tcttatagat agaccatggg taacgataaa aacaaaaaag
    37201 aacttgcgac ttattttgcc gtaagttctt ttttagctag tgccttaatc tcttaatccc
    37261 aacaaacgtc tcgcatagat cacctgacca gagtgcatag cagcatcatc aataatagaa
    37321 acgagacgca ttcctcgtgt gactggtggt gtccagttct cgtcaattac ttcatctagc
    37381 tcggaaggtt ccagccttct taagtaagaa atagcaaaat cagcagctcc tcgcaagtat
    37441 cctagcagat cttctatttg attagcttgg atctgctttg cttgttccag actatgcttc
    37501 caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc
    37561 caaattggag aagttcctgc taaatcagca atctggaaat ctaattctcg agcggtatgc
    37621 caagccagcc aagtcattga tttgatctgc ggggctctat cttccactgg aaaacgattg
    37681 gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca
    37741 ctctcgcata gcatatctaa ataatccatt tattttcctc ctttttattt atcctactta
    37801 ctttttccta attctaggat acctacttat atgttactac acacccttat tttttacact
    37861 ttaacagtca ccatcatcaa tagtttggag attgaaagaa aaagatagtg tcgaactatc
    37921 aattgaacaa gaagcaacaa tatgaacgtt tcattaatat tctttaaata aacaatgatt
    37981 tttcaaacga acagtttaag actaggagaa aaagagtata ataggaaaat gtttgaaagg
    38041 aaaggaaggg gaaaaatgaa aaaaagggca agtttactag tcctagttgc tttgttgttc
    38101 aatatctttt cgtatggtgt ggccgtttca gcggaaacat acattcctaa agaccctggg
    38161 aatatttata atgatctaac tggtaactat ggcttgttag gaattgctag tcaattccac
    38221 gtatttgcta aaaataagac aactgtgaat gcacatacag acggaaatat agcaacacat
    38281 gaacttgatg caaaaaataa ttttggtaca gagcttcatg aaggtttatt gaaccaagaa
    38341 ataaattata tacaaaaaac tgattctttc gctaattctt caggcatacc tacaggtagc
    38401 acaagaatga ataaatttgt cgttggcagt aacgtggatg tagcacttca agatggtcat
    38461 cctacagtca atggatccag gcttgatcac ttaactccag atgattttta taaagatcgt
    38521 gctgggcatg aatatattga tttcgagcag gaatttgcta aattgaatgt agcagcaaat
    38581 gatcttgcaa cgatcactcc agctaaaaca tatactgctg cagattttcc agacatgaac
    38641 aatcggacaa tcgatttaac tgatttatca gataccgggt ttttattggt aaatatcgat
    38701 gcagaagtgt taacgatgaa tacgccactt caaatcatta atccaaatga tcaagtagtt
    38761 gtatttaatg tcatcaacag ttcttctgca ttgaacgtac agtcaccaat caaatacaat
    38821 gatcgttcaa atcatgaaac agaagatttt tcggatgcga atatctcttg gaattttgga
    38881 aatgagatga ctgatttgac aatcagtgct ccttttcaag gaacaatcct tgcgcctaat
    38941 gctactatcc gcattactca aaaccaagac ggaacgatta taggaacaaa tgtgatttta
    39001 gatgcagcta ccaatcgttg ggatcctaac gaaatattta tcagcgacaa tggtacagat
    39061 acgacagaca caagcgacag tacagataca tctaccacaa gtgatagctc agattcatca
    39121 acttcaagcg acagtacaga tactacctct accacaagtg atagcacgga tacatcagct
    39181 tcaagcgaca gtacagatac tacctctacc acaagtgata gcacggacac atcagcttca
    39241 agtgacagta cagatactac ctctaccaca agtgatagca cggacacgtc agcttcaagc
    39301 gacagtacgg atacatctac cacaacaagt gatagcacgg acacgtcagc ttcaagtgac
    39361 agtacagata ctacctctac cacaagtgat agctcagatt catctaccac tacaagtgat
    39421 agcacggaca cgtcagcttc aagtgacagt acagatacat cctctaccac aagtgatagc
    39481 tcagattcat caacttcaag cgatagtaca gacacgtcgt ctaccacaag cgatacacgt
    39541 catgcaagtg tctccttctc aaaaacaagt tcggataaca ggacgaactt taacttaact
    39601 aataaaacaa gcaatgacaa tggaaacaaa tcaaaaagag cacttcctaa aactggaagt
    39661 caatcgaata attggataac actcgcaggc gttatattac ttgtaatcgg tttaagaatc
    39721 acttttatga gttataagaa atcgaaaagg taaaaaaata ttcaagataa aaaagagcaa
    39781 cgtcgctaac ggacgttgct ctttattttt gctttttatc ataaatacaa gaactacaat
    39841 tcaatttgtc aaaaaacacg tagatttcct tctgagaaat ttatttacgt atcgaacctt
    39901 tccctattca gtctttgcga tccaaaatca gttgggtaag ggaaaagaga ttctcttttg
    39961 ttcgaagaga ggtggcaggt aatttcttta tttccttcaa agccttttct gtgtaagagg
    40021 ccgctaactt cctagcttct tccacgcctc ctgtatgttc aacgattttg cgtacggcgt
    40081 cgacatcttc ttctgtcatt gccgctttct tctctaaata aggtagtagc tttggtttgt
    40141 ttttttgcaa agagtagatc aacggtaaag aatagacccc ttgacgcatg tcttctagta
    40201 caggtttgcc gatttcctgg ctctcttttg tataatctaa aatatcatcg ataatctgaa
    40261 aggccatccc gatagaaagt ccgatatctc ctgctttttt tgcgaagcgc tcgcttgttc
    40321 cactttcaaa agcgccaaca gaacaactta attggaacag ttcagctgtc ttacctgaaa
    40381 tattttccaa atattctttt acagaaagtt cataattata gcgatcatcc atctgcccta
    40441 attcgccatt caatactttt tccatactgc gagaattcaa ttggagactt ttcaaggaag
    40501 tggagtaatc agacaatagt ttaaaacagc agacaaataa atagtctcct gcatagacag
    40561 aaacgacatt accaaactga ctacggatat taggctgacc acgtcgcaga tccgcttcat
    40621 caacgatatc atcatggatc agtgtggccg tatggagcat ttcgatagaa gctgccaaag
    40681 cgatggcttt gtctcgatct tgctctggtc caaattgtga gaacaatagc tgataggcag
    40741 gacgcaacag ctttccgccg gcatggatcg tttctttgat tgcttgttcg acaggtttgt
    40801 ttttcaggcg gacagtttct tccatccgtt ttagtgtttc tgtcaattct ttacttaaag
    40861 caggatagtt ttcccacatt ggatgtagct tcataaaaaa actccttgtg tgacgattat
    40921 tttttggtaa acattgggct ccatttttgt ttggttccct gctcataatc tttttggaat
    40981 cgttgaagtg tctgcacatg tcgtaaaaga taattcgctg cgatcccgat tgcaatccct
    41041 gataaaatcc ctaaaactga aaggactggc aagtacaaca tgactgtcca tgattgtgca
    41101 atgaaagaag ccatcaaaag ctgtccgaca ttatgcatga aaccgccggc cgcactaatt
    41161 ccgatgatac tgacacgttt tggtcctaat tgcttgacca ataacattcc aaaataacta
    41221 agcaaagcac cactcatact ataaaggaat gtggaaatcg ttccgcctag caaagtagtc
    41281 aggaatagtc gtagccaaag tagtaagaag ctgtctcgtt ttttcatggt aaaaatggca
    41341 acgatcgtga ttagattggc taagcctagt tttgcaccag gcgcaaaaga aaaaggataa
    41401 gggatcatat tttccaataa accaatgatc actccttgtg caacaagtag cgaaatataa
    41461 atcatttttt gtaatttact catggtgtgt gaaggtctaa taaatcagac tgtcttcgtc
    41521 acctccatct gttgaacgga cttcgatcac taatttatgt ggcaaacaga cgatggtttc
    41581 gccatttttc gttgcccagc ctcgacgtac gcagatctgg tcatcgcaat ctgcttcttt
    41641 tatccgtact cgatcgccat ctatctcgat caagttataa tctccgtgtt catctttgta
    41701 agtatatgtg ttgctctctt ctccagccat caaagggaat gttttgattt ctgtgccatc
    41761 cacacgcagt accgcttctt tttctgctgt tgcggtgttt tgctgaagac caaaaatcat
    41821 gactggtaaa aaagaaagaa cagtcaaaag caaaataata aaaacatccc aaggtttgag
    41881 cagacttttt tcagcaaatt ctttaaggct catttttgtt ctcctttttc cagattaact
    41941 cacccaattt tagcataaaa acaagaggtt gtgacacctg tgtcttgctt tgagtaataa
    42001 gaacgaagaa tagaaaacag ctttccatgt tttctaattc tttgggctta ttaccgagaa
    42061 gcagcaggtg gaacaccgtt tataacaaga ggttgtgaca gaagtgggca acttcaagaa
    42121 ataagaagga atttacgaaa attacttttc aagtttttgt gaaatttcgg cttatttttc
    42181 gaagaagcta cttctggaac accgtttata acaagaggtt gtaacagagg ttgtgacacc
    42241 tgtgtcttgc tcatggaggc ttatctcggg catcttattc ctttaatttt acataaaaag
    42301 gaggctgtga cggtttgtct cagccccttt ctattgaaat cttgcattca aataacgttc
    42361 tttatctatt tatttcttgc cgtactggga acttggtcta ccgtaccacc aattacctag
    42421 tgtacgttgt agccatggaa ttacccagcg atccaaccca atcgcacgtc cggaaccgtt
    42481 catcaatgcg aaagcaacaa aaatgaacca aatgtttacc cagtaaaaca tgcctgataa
    42541 gcagaacgtg atcaccaata caatcgttgt cgcactgcat agccatgtga ataatccagc
    42601 aatcaatgct aatgccaagg caatttcaaa gaataccatg aatttttgga agaacattgc
    42661 tacatcattg tttggcatga tcacttgcat gatctttgtg aaccagtctg gtgacttctc
    42721 aatcacagcc attggttctt caccgtatgc ataactcaaa ccaaaatgtg cagcttgagt
    42781 cgctgcttct ccggcacttg cccctgctcc gctggcagct gtggttgcat caccagcagc
    42841 agaagcacca ctggcaacat ctgttacatt gtattcccat gtccaaggga acacactgtt
    42901 tccagtgaac catgatcctt caccaaacca agtacttggt ttgaacactt caccattccc
    42961 aatgattttt ttggaagctt caacgaacca aactaaacca tagaaaatac gaagtggcac
    43021 actccataag acattgccgt aacgagaaga atgtcctcga gtcacgttgc gatcatcttt
    43081 gatatggaaa aactcatgca ttaaatattg gaacatataa taaccagaac gaatatcaaa
    43141 gaagtatttc aaattcacga tatgtttcat tacgatagct aaaaatccgc ttaaatggat
    43201 cttatcaaat aggttagcta ccccccattt ggcaccaata gaaaccataa atccttgata
    43261 gtttccttta aatttatgct tttctccgcc tttgatcgaa gccacgatat tcgctgcggc
    43321 tgtgtgtcct gtttgttcag cagcttgaac gatctgcggt gtcggtgttc ctgggaattc
    43381 ttcataaaag actaaatcac caatgatata gatgttttta tcttcgtagc cttttgcctg
    43441 catatattca ttggcgatca accgctgtcc gcgtgctgat tctaatccaa agtcagcagc
    43501 gtcagaagtt cctttgacac cagctgtcca aatcagtgtg tgagtcggaa tgttgcgtcc
    43561 gtctttcaat ttgatatgat cagcagccac ttctacgatt ggtgcattca ataataattc
    43621 cacgttgtgc ttacgcaaat agcgttcagc ttttgcagca tcatttcgat ccaacatatt
    43681 caagatcgtc ggcattgcct ctacgaccat cagtgtgatt tcgtttggat ctaatttata
    43741 atctttggct aggcgatttt tccagtcgat cagttcccca atcatttcaa taccggtaaa
    43801 tccagaacca cagacaacga aagtcagcat ggctttacgc agttcatcat ctggttccaa
    43861 tgcagctttt gctactgtct tctcaatatg ttcccgaatc cttacagaat cttcaaatga
    43921 ccaaagggtg aatccgtgtg ctttgacacc aggtgtacca aagtcattcg gctctccgcc
    43981 cattccaagg atcagctgat caaaaggata ggaacctaat ttggtcttga cgatttttcg
    44041 ttctttatcg ataccgatta cagtgtctgt gacaagttgt acattctttt gacgagcaaa
    44101 caaatgctgc agatcgtatt ggattgcttc tggttctaca cgtccgccag ctacttcatg
    44161 caactcggtc atcatagtat ggtacgaatg acgatcgatc agagtaattg ctacatcttc
    44221 ttttttcagt ttctttgcca agtattttgt agctgaaaca ccggcatatc ctgctcctac
    44281 aacgacaata ttttgctttg ccattgatca tccgctcctt atagactatc tatataatga
    44341 atatatgata attcttttag tttgatactt attacacaat ctccatatat tatatagatg
    44401 ttctgttctt ttgtctaact ccttttggat ttttttcaaa ttttaaaata aagcgttttt
    44461 tcacgaaatt tgctgaaaga aaacgtttga aatattacat gggttattta tcaaaaatag
    44521 ttgacttata ggctaaaaag attagaatgt ataatgaatc acaaagaaaa attgtggact
    44581 agaaatggaa tggtggggta ttaaaatgaa gaaaaaacgt tttgtaactg gatttgccgt
    44641 attagcattt tcagctctag tgttaggcgc ttgcggcgca gatgacaacg gaagctcaaa
    44701 ctcttcttct gaatcatcta cagcacaatc ttcgactgca aaaagcacaa cagaatcatc
    44761 tgcaaaagtt gtagctggcg gagatttgca agacggcaca tataaattag aagaaaaaaa
    44821 ttattcaaat ggttaccgtg cagtctttga aatggttgta aaagacggca aaatcactga
    44881 atcaaaatat gataacgtca atgaaaacgg cgaatcaaaa acaaaagatg cagaatacaa
    44941 caaaaacatg gaagcaaaat ctggcacaaa cccagaaaaa tacattcctg agttgaatga
    45001 ccaattctta aaagcacaaa gcgcaagcgg tgtggaagtc gtaactggag ctactcactc
    45061 atctgaaagc ttccaaaact acgcacaaca attgatccaa gctgctcaag ctggtaacac
    45121 agatacaatc gaaatcgaca atggtgctga tttgaaagac ggcacttaca agcttgaaga
    45181 aaagaactac tctaacggct accgtgtgca attcgaaatg acagttgctg gcggcaaagt
    45241 aactgaatca aactttgatt atatcgacaa agacggtaaa tcaaaacaag atgatacaga
    45301 atataatgaa aatatgaaag caaaaagcgg tacagaacca aaaacttaca tccctactct
    45361 gaacgacgaa ttcgtcaaag caatgggcga agaagatggc tcacctgcta atgtagaagt
    45421 tgttacaggt gctacacaca gctcacattc attcatcatg tacgcacaac aattagtgaa
    45481 tgcagctgaa aaaggcgaca ctcaaacaat cgaagtagat aatatcgtaa cagaaaaata
    45541 atcacataat catactaata aaagttggag ggtgggacga tagccagatt aggcagtcgt
    45601 ctcacctttc aaaatgaaga gaggaaaaag atgcggaaaa aactactatt ttccttaatc
    45661 acactgggca tctcttttat ggtgctaact ggctgcagca actcagcaaa taaaacaacc
    45721 actgaaagtt cagcagctac caagatattg aaagatccat attctgatga acagttcttg
    45781 ttagggactt acgttcgtat ccgtgtgtat gatgaaggca aagaatcagc attgaaacct
    45841 gccttcgatc gagtaaaaga acttggtgat aaaatcacga tcaatcaaaa aggttctgag
    45901 atcgatgaag tgaatgagca agctgggatc aaaccagtaa aagtttctga tgacgtctac
    45961 acgttagtca agcgggccta cgaatatagc caagattctc aaggcggatt cgatatggcg
    46021 attggtgcaa tcacacaatt atggcgaatc ggttttgatg acgccagaaa accttcccaa
    46081 gaagaaatcg atcaggcatt gaaattagtg gattatcata agatcgaact aaatgacaaa
    46141 gaaaaaactg tttatctaaa agaaaaagga atgatcatcg atttaggtgc aatcgccaaa
    46201 ggttacatta cggacgaagt agtgaaagtc ttgaaaaaac aaggagtgac cactgccatc
    46261 gttgacttgg gcggtaatgt ctatgtctta ggccacagcc caagaggcga aaaccaagac
    46321 tggacagtcg gcatccaaga tccgaacatg gctcgcggat ctgttttagg ttctattaaa
    46381 gaacggaaca aaacattagt tacctctgga atttacgagc gttatttaga agtagatggc
    46441 aaaaaatacc atcatttgtt tgatccaaaa acgggttatc cgttcgacaa tgatatcgca
    46501 agtgtcacga tcataacaga taaatcaatt gatggtgacg ggttgtctac agcagtcttt
    46561 tcaatgggtg tgaaaaaagg actggaatac gtcgaaagcg aattgaataa cgggacggaa
    46621 gcgatcttcg tgacaaaaga cgataaagta tatgtgaccg atccaatcaa agacaccttc
    46681 aaattatcgg aggactccca ttacacgatg ggtgaccgtt ctgaactaaa ataaggagta
    46741 cgaaccatga cactaaaaat ctttttagaa gtagttgaaa tacgaacaaa ggtggctagt
    46801 gtatttcctt ttgcggtagg ggtattattc tctatcgctt tttttcatga gatacattgg
    46861 ctaaacactt ttttattttt tattggaatg ctagtttttg atttagcgac taccgctatc
    46921 aataattaca tggattatga aaaggctcat tctgaagtat ataagtatga agaaaatgtc
    46981 attggccgca gaaatctatc tcctgtattg attcgaaaca tgattttagc tatgattgca
    47041 tttgtttttg ttatcggcgt tattctaacg atcaatacgg gttggctttt acttttgatg
    47101 ggatttatct gttgttttat tggagtattc tatacattcg gaccgattcc tttatctcga
    47161 atgcctttag gggaaatttt cagtggggtg actatgggcc ttggaatttt tgccatggtc
    47221 gtttatgtca atacattcgg ttcagatgtc tatgatctga cagtcaactt ttcatctgga
    47281 caatttgctt tggtaggaaa tctttgggct atagcagcaa tcgtattagc ttccttaccc
    47341 ttggtgttta cgattgccaa tatcatgttg gctaataatt tgcgggatct tgaccgtgat
    47401 attgaaaatc atcgctatac actagttttt tatatcggac gtcctgcggg aacagtctta
    47461 ttccagctat tgatgtatgc ttgctatttc gtgattttaa tggggttgtt gagcggtatc
    47521 tatgaatggc cgatcttggt caccttcttg accttgccaa ttgtgaagaa gcatttggat
    47581 caattcaagc aaacgttacc ccagcctatc agtttcagct attccatcaa aaatatggtc
    47641 ttattcaatg ccagttatgt attggggcta ttactaacaa tcattatgca agccatttaa
    47701 cagcatgaag aggttgtgaa aaaactgccc tgcatcaagt aataagaaca gccaaataaa
    47761 aatagctcct tatatttttc gtttggttgg gcttattacc gcagatgcaa gcttttgaac
    47821 accgtttatc aggaggttgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt
    47881 gaaaatggct ttccacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
    47941 acaccgttta tcagaggttg taaaatcagt gttttgtcct gagtattaga gaaacaaatg
    48001 gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggac agctgattga
    48061 acactatttg ttcggaaaaa gtgactaact atgacacaat ttttttcaca gtcccttttt
    48121 tcacttgatt tgattcgtac cagcgaactt gacgagaact ttcttggttt ggtaactata
    48181 tggtattctt ttaacatcct cgtttgcttc ttctaattta tttatacaaa gtcacacttt
    48241 tttaaacatc tgttcttcga tacatttcta tttcttttaa acatcgatac caatattttg
    48301 aacttaagag tgcataccaa atggattcat ttactcttcg tcagttacga agataatcaa
    48361 aatctccaac ttccctactt atttcgtttt gagaattatc gaagcaatct tacataaata
    48421 actaaaaaac aaaaaaccag aaaatcagta tactgatttt ctggttttac atggtttgtt
    48481 caataaaaag ctgttggaac tgttaatatt aaattatcgc atcgttacga attcttccgc
    48541 tcctgttgga tggatggcta ctgtattgtc aaagtcttct ttagttgcac ccatcttgat
    48601 agccacggca aaaccttgca gcatctcatc gacaccaaca ccaatagcat gtaagccgac
    48661 gattctttct tcttttccga cacagatcaa tttcatttcg catttttggc gataatcatt
    48721 taatgcaaag tacattgggg taaagcgtga acgataaact ttcaattcat tttcgccata
    48781 tttttccaat gcttcttctt ctgtcaaacc aattgttgca attggtggat gcgtaaagac
    48841 gactgtcgga acaagtttat agtcaagata agaatctttc tttccattaa ataaacgttc
    48901 tgataaacgt ctaccagctg cgattgcaac aggagtcaga tctaatttac cgatcacatc
    48961 accaacagca aaaatatgtt catccgttgt attttggtat ttatctactt tgataaaccc
    49021 ttttttgtcc aattccacat ttgtattttc cagacctaat ttttcggtat tcggtacacg
    49081 tccaccggca aaaagtacac aatcaccagt taaggtttca ccattttcaa atagcaccgt
    49141 atattcgtca ttgtttttag taatttcttt agcaatatga tgtgcgtaag tttttattcc
    49201 agcttcttgg tacatttgga tcaaattgtc cgaaagcatt ttatcaaacg ttctaagtgg
    49261 tcgttctttt cgatatgccc acatgacttc actacctaaa ccacgaaata cacctgaaag
    49321 ttcagccgca atgtatccag cacctaaaac gattgctctc ttaggtaact ctgttaattc
    49381 aaaaaatcca tttgaatcaa ttgcgtattc tccaccagga atggtcattt tacttgcacg
    49441 gcctccagta gcgatcaaga ttttaggagc acgataggtt tcaccgttta cttcgatcgt
    49501 ttgactatcg ataaaagtag cgtaaccttt tatagcctcg acatgattgc tatctaatcc
    49561 tctttgataa gcaccgtgta gaaaatcaat atatttttct cgtctttcta ctagaccggc
    49621 gaaatcaaca ttttttaaat ctgcttggat accgtaactt gaggcatctc gttcaattgt
    49681 ttccaaaatc gtacttgctt gccacataac ttttttaggt acacaaccga cgttgacaca
    49741 tgttccgccc aattcattcg cttcaatcag caaaactttt gcgccatgca tccctgctct
    49801 gtttgctgaa gcaataccgc cagagccgcc accaattacg atgtaatcat atatttgcat
    49861 tttgttcctc cttattgaag agatcaatca aaacctttat ttaacgatcc tttttctaca
    49921 gaaaacaagt gtaacacttt tcttgaattt tgtatgcgaa gatgcttaaa acagggatat
    49981 atcagctttt tccttattta cgttttcgga tcatatctcc aggtttcacc ggctgtgcga
    50041 ctggcatagt taaaatcatc attggatttg gtgctcggtc aatggattcg ccttcttcat
    50101 tatgcataac ttcaacggtt tgactgaaat gactgaatcc tggaccataa aattcgattt
    50161 catctcctac agaaaacaag tttctctgac gtatcgtagc aatctgtgtt gcctcatcat
    50221 aagataatac ttcaccgaca aatttatata ctgggatttt acgacgttca ccgaataatt
    50281 gttcgttttc agttggtgta ttgtagtaaa aacctgtagc taattctcgt tgtgctactt
    50341 tccataactc gtcgatccat tcttgcttgc agacatagtt ttcaggatct tccatataac
    50401 tgtctacagc cgctttatag acatttgaca ccgtagatac ataatggatc gatttcattc
    50461 ttccttcgat cttgaagctg tctacgccgt tttcaatcaa ttctggaata tgttcgatca
    50521 ttgacatatc cactgcactc atcgagaatt cttcttccac ttcgcctgta tcagttaaac
    50581 tgtttcgttc ttgaccaaaa ggcatatcga acaatccata cttccagcga caagattgag
    50641 aacatccgcc acggtttgca tcccgcatcg acatatggtt ggacaatgtg catcgaccag
    50701 aataagagat acacatggca ccatggataa atgcttcgat ttcggtatcc gtatttttac
    50761 gaatctctgc cacttcctcc attgaaactt cacgagctaa aacgacacgt tccaaccctt
    50821 cttctttcca gaattccaat gtctcaaaat tagttgctga agcttgagtg gataaatgga
    50881 tcggcagacc tggggcttct gtcgcacaaa tttcgatcaa agctggatca gaaacaatca
    50941 ctgcagaaat accgacatca cgcaaactgc ggaaaaattc ccctgctcca tcttcatttc
    51001 cttcatgggt gaccatatta gcagcaacgt ataccttcgc atcatgttct tttgcaaaag
    51061 caactccttc agccatttct tcataagtaa aattacctgc acggcttctt aaaccatatg
    51121 cattcccgcc aatatatact gcatctgctc cataatggat cgcggttttt aacttttcca
    51181 gtgttcctgc tggcgctagg atttctggtc gtttcaattt tctttttact gtcatttctt
    51241 tcatcatact ccttatttga tagcagaagg atcaatataa aagaatcctg tatctaattc
    51301 acggttttca ggatgcaatt tactgatttc gctagtcatt ttttccgctt tttcttgatc
    51361 ccagctacct tctaaaattt gtttttttgc ttcggcgaat agtcgagtga tgtttacgaa
    51421 tttttcgcct ggagtaaata atccgtccaa tttccatgta cagtaatgat gctgataaag
    51481 ttcattcaat tcattcgaga gattcagatc attgctggca aaaatatgtg tgccatgact
    51541 atcttcataa atagaataat gtgtctcttc ttttttaggt tccgagataa aaagacctct
    51601 ttcacgatct ttttgttcgt cttgtttcgt atagttatag taattctgta gcagtggacg
    51661 tttggattga tggatgcatg tagcaccata cacaagggtt tctacaggaa tatcgagttt
    51721 ttcttccatc gccttcatct cttcaaaagg aacttcacga gcaagtactg ctccaactgc
    51781 gccttttttc gcccagaaat tgatttgacg tgcgctcgtt actaatgttt cagcatcata
    51841 aatatacggt atacgcaatt caggattttt tttcatcgta taaataatac ctgtatcacc
    51901 aactgtgatc acatctacgt ttatttcttt taaaaaagcc aaatattcag gtacattttt
    51961 cattttttca ggatgaatga tcccatttac agcaatattc gcttttttac catacttgtg
    52021 tgctaattct accaattgtc gctgttcttc tcgtgtaaat gaagcaggca gacgcaatcc
    52081 aaattgctct tcaccgaaat atagtgtatc tgcacctgca gcaagcaact gttctgcttg
    52141 ttcaatagtt tctactgtgg ttatgagttc gatcataatt ttctcctcct acaacttcat
    52201 aatagtagca tagctctaaa aactttccaa tacttcttct atttaaagtc ggatttttga
    52261 aaattatttc acaaaaaatc aaaataaagt tcgatatatt ctgtccaaat ataaaaagag
    52321 attgtgacaa aaatcttgtc acaacctctt aatcaagtct tactattata aaaattcagc
    52381 tacacaggcc aagtaactat agggaaccta ctgtttcctt tttaggtccg cgaacattca
    52441 atgtgatctt accttttgaa gcacctactg ttacgtggtc acctaaacga atttctccgc
    52501 caagcaaagc ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga cggattggac
    52561 gtgcaccata ttcaggatca aatccagctt tcccgatcac atcgattgca gatggtgtga
    52621 ttttcacttg aatatcttgt tctttcaatc gtttgataat cgctttgctc atgatttgga
    52681 cgattgtatg gatttcatct ttactcaatg aatggaagac gacagtctca tcgattcgat
    52741 tcaagaattc aggtcggaaa gctttcttta actcttctaa gatacgtttt tgcatggctt
    52801 tgtgatcttt agtaacatct tggacattga atccgacatt tttttcttct cgaatttgtg
    52861 tcgctccgat atttgaagtc ataatcatga ttgtatttct aaagtccact tttctacctt
    52921 tagaatctgt taaatgacca tcatccaata cttgcagaag taaattgaaa acgtctggat
    52981 gtgctttctc cacttcatct aagagaatca ctgaatatgg tttttgacga attttttctg
    53041 tcaactgtcc accttcatca taccctacgt atcctggagg agacccgatt aatcggcttg
    53101 tactgtattt ttccataaat tctgacatat caacacggat cagggcatct tcacttccaa
    53161 acatgacttc tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt ggtccaagga
    53221 acatgaatga accaattgga cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg
    53281 cacgtgaaac tgctttgaca gcctcatctt gaccaacgac acgttcatgc agaagtcctt
    53341 ccagttctaa caagcgttcg ctttcttttt tctccaactg ctgtagaggg acaccagtcc
    53401 actccgatac aactgtagca acatcttctt ctgttacacg atcagaataa ccttgctcct
    53461 gttttgcttc aattgcagaa actttttgca gctttttacg tagtgctttt tcttgtctgc
    53521 gtaattgagc agcattttca aagtcttgtt tttggatcgc tgcttccttt tcttgtacta
    53581 gttcatcgat ttctagtttg atcacagttg attttgttaa gtgatcggtt tgatccaaac
    53641 ggactttcgc tgctgattca tcgatcaaat caattgcttt atcgggtagt tgacgagaag
    53701 tgatataacg aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg atctccactc
    53761 catggtgctc ttcatatctt gtacgtaatc cttgaagaat cacttctgct tcttcaggag
    53821 ttggctcatc tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat
    53881 atttttgata ttcatctagg gtcgttgctc cgatcgtttg taattctcct cgagctaacg
    53941 ccggtttcaa gatattcgaa gcatcaattg ctccttctgc tccacctgca ccaattaagg
    54001 tatgcaattc atcaatgaat aagattactt gaccatcatt atagatttcg tcgatgacct
    54061 ttttcattcg atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa gatcccatat
    54121 caagcatcat caggcgcttt tcttgcatgt cttctggcac ttcacctaaa atgatttttt
    54181 gagctaaccc ttcagcaatg gctgttttgc ctacaccagg ttcacccacc agtacagggt
    54241 tattctttgt ccgtcggcta agaatctgga taagccgttt cacttctctc gaacgaccaa
    54301 caactgggtc cagcttgttt tcttttgcta atttcgtcaa atcacgtgct aatgaatcta
    54361 gcgtaggtgt tccttgcggt gtttgccgtt tatcttgtac atttacattt ctacgacgat
    54421 tcattccttt tccagcagaa tcaccagcta gccccatttt ttgtttcaga acttgacggg
    54481 cttttgaaag gctgatacct aagttgatca tgattcgagt agccatgata ctttcatctt
    54541 ttaataagct catcaaaata tgttctgttc caactttttg agaaccagtt ttcttcactt
    54601 cgctgctagc taaagcaaaa acttgtttca ttcttggtga atatggaaga aagactcctt
    54661 tagggtaaga acggatacca ccataaccaa tcagatgttc aatttcttca taaatttccg
    54721 tttcattcaa atccatttca cgtaatactt ttccagcaat tccattttgc tcgaccacta
    54781 aggctagtaa aacatgttct gaactaactg attgatgttt caaccgtttt gcttcttctt
    54841 gtgcaatttc taatactgct tgggctcttt cagtaaaaag ttcttccatc taatctcact
    54901 tcccatcttc ataacttaaa cgttccagaa atgctcggag aatccgagca cgtgtatgat
    54961 cttcatattc attgaaattc aatgtgtttt ttgcaattgc actcaacatc aagtttcctt
    55021 ctttttttgt aattatttgg tcttcataaa gtttttgaat aatagcaaaa gcatttttct
    55081 cactgattgt atcatcaaat aactgattta tctgctcaag taattgtttt ttatctgaaa
    55141 tccttacttt tgcaattcgt atataaccac caccaccacg tttgctttct actagataac
    55201 cacgttggat ggtaaaacgg gtattaatca catagttgat ttgtgaagga acacagttaa
    55261 agaggtctgc catttcaatc cttcgaatct cgatcatttc actattttcc agaattttct
    55321 ttatatatga ttcaattaaa tccgaggtat tctgatgtgc catcttattc attccctttg
    55381 actaatattg accttaatta taacgaattt gataatagaa agaaagaaga acgctttttg
    55441 ttaaataaat cctttagaat caacaaaaaa aaatacggaa tatactattc cgtatc
//
LOCUS       DAZKOK010000005        49925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000005 DAZKOK010000000
VERSION     DAZKOK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.005"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1367
                     /locus_tag="ACG90W_000288"
     CDS             147..1367
                     /locus_tag="ACG90W_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0476685.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1364..4084
                     /locus_tag="ACG90W_000289"
     CDS             1364..4084
                     /locus_tag="ACG90W_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0476686.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4068..5012
                     /locus_tag="ACG90W_000290"
     CDS             4068..5012
                     /locus_tag="ACG90W_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0476687.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5081..6091)
                     /locus_tag="ACG90W_000291"
     CDS             complement(5081..6091)
                     /locus_tag="ACG90W_000291"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0476688.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6271..6615)
                     /locus_tag="ACG90W_000292"
     CDS             complement(6271..6615)
                     /locus_tag="ACG90W_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0476689.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6608..7042)
                     /locus_tag="ACG90W_000293"
     CDS             complement(6608..7042)
                     /locus_tag="ACG90W_000293"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0476690.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7180..7917
                     /locus_tag="ACG90W_000294"
     CDS             7180..7917
                     /locus_tag="ACG90W_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0476691.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7910..9121
                     /locus_tag="ACG90W_000295"
     CDS             7910..9121
                     /locus_tag="ACG90W_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476692.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9325..10107
                     /locus_tag="ACG90W_000296"
     CDS             9325..10107
                     /locus_tag="ACG90W_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0476693.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10177..10839
                     /gene="trmB"
                     /locus_tag="ACG90W_000297"
     CDS             10177..10839
                     /gene="trmB"
                     /locus_tag="ACG90W_000297"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0476694.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10937..12691)
                     /gene="ade"
                     /locus_tag="ACG90W_000298"
     CDS             complement(10937..12691)
                     /gene="ade"
                     /locus_tag="ACG90W_000298"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0476695.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12823..13752
                     /gene="comGA"
                     /locus_tag="ACG90W_000299"
     CDS             12823..13752
                     /gene="comGA"
                     /locus_tag="ACG90W_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0476696.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13709..14758
                     /gene="comGB"
                     /locus_tag="ACG90W_000300"
     CDS             13709..14758
                     /gene="comGB"
                     /locus_tag="ACG90W_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0476697.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14755..15033
                     /gene="comGC"
                     /locus_tag="ACG90W_000301"
     CDS             14755..15033
                     /gene="comGC"
                     /locus_tag="ACG90W_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0476698.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15008..15478
                     /gene="comGD"
                     /locus_tag="ACG90W_000302"
     CDS             15008..15478
                     /gene="comGD"
                     /locus_tag="ACG90W_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0476699.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15450..15797
                     /locus_tag="ACG90W_000303"
     CDS             15450..15797
                     /locus_tag="ACG90W_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476700.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15760..16185
                     /gene="comGF"
                     /locus_tag="ACG90W_000304"
     CDS             15760..16185
                     /gene="comGF"
                     /locus_tag="ACG90W_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0476701.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16157..16516
                     /gene="comGG"
                     /locus_tag="ACG90W_000305"
     CDS             16157..16516
                     /gene="comGG"
                     /locus_tag="ACG90W_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0476702.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16688..17695
                     /locus_tag="ACG90W_000306"
     CDS             16688..17695
                     /locus_tag="ACG90W_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0476703.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17720..18904
                     /locus_tag="ACG90W_000307"
     CDS             17720..18904
                     /locus_tag="ACG90W_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0476704.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19341..20924)
                     /gene="abc-f"
                     /locus_tag="ACG90W_000308"
     CDS             complement(19341..20924)
                     /gene="abc-f"
                     /locus_tag="ACG90W_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0476705.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21029..21157)
                     /locus_tag="ACG90W_000309"
     CDS             complement(21029..21157)
                     /locus_tag="ACG90W_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476706.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21502..21645)
                     /locus_tag="ACG90W_000310"
     CDS             complement(21502..21645)
                     /locus_tag="ACG90W_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0476707.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21868..22347)
                     /locus_tag="ACG90W_000311"
     CDS             complement(21868..22347)
                     /locus_tag="ACG90W_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0476708.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22420..22704)
                     /locus_tag="ACG90W_000312"
     CDS             complement(22420..22704)
                     /locus_tag="ACG90W_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476709.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22782..22976)
                     /gene="ssrS"
                     /locus_tag="ACG90W_000313"
     ncRNA           complement(22782..22976)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG90W_000313"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23048..24334)
                     /locus_tag="ACG90W_000314"
     CDS             complement(23048..24334)
                     /locus_tag="ACG90W_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0476710.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24472..25143
                     /locus_tag="ACG90W_000315"
     CDS             24472..25143
                     /locus_tag="ACG90W_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0476711.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25109..25597
                     /locus_tag="ACG90W_000316"
     CDS             25109..25597
                     /locus_tag="ACG90W_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0476712.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25610..26557
                     /gene="prmA"
                     /locus_tag="ACG90W_000317"
     CDS             25610..26557
                     /gene="prmA"
                     /locus_tag="ACG90W_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0476713.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26558..27322
                     /locus_tag="ACG90W_000318"
     CDS             26558..27322
                     /locus_tag="ACG90W_000318"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0476714.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27555..29768
                     /locus_tag="ACG90W_000319"
     CDS             27555..29768
                     /locus_tag="ACG90W_000319"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0476715.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29779..30225
                     /gene="dtd"
                     /locus_tag="ACG90W_000320"
     CDS             29779..30225
                     /gene="dtd"
                     /locus_tag="ACG90W_000320"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0476716.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30396..31655
                     /locus_tag="ACG90W_000321"
     CDS             30396..31655
                     /locus_tag="ACG90W_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0476717.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31744..32691)
                     /locus_tag="ACG90W_000322"
     CDS             complement(31744..32691)
                     /locus_tag="ACG90W_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0476718.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33085..34377
                     /gene="hisS"
                     /locus_tag="ACG90W_000323"
     CDS             33085..34377
                     /gene="hisS"
                     /locus_tag="ACG90W_000323"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0476719.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34392..36161
                     /gene="aspS"
                     /locus_tag="ACG90W_000324"
     CDS             34392..36161
                     /gene="aspS"
                     /locus_tag="ACG90W_000324"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0476720.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36270..37145
                     /locus_tag="ACG90W_000325"
     CDS             36270..37145
                     /locus_tag="ACG90W_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0476721.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37411..39294
                     /locus_tag="ACG90W_000326"
     CDS             37411..39294
                     /locus_tag="ACG90W_000326"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0476722.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39369..39653)
                     /locus_tag="ACG90W_000327"
     CDS             complement(39369..39653)
                     /locus_tag="ACG90W_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0476723.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39643..40377)
                     /locus_tag="ACG90W_000328"
     CDS             complement(39643..40377)
                     /locus_tag="ACG90W_000328"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0476724.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40506..41129
                     /locus_tag="ACG90W_000329"
     CDS             40506..41129
                     /locus_tag="ACG90W_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0476725.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41321..42442
                     /locus_tag="ACG90W_000330"
     CDS             41321..42442
                     /locus_tag="ACG90W_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0476726.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42449..45577
                     /locus_tag="ACG90W_000331"
     CDS             42449..45577
                     /locus_tag="ACG90W_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0476727.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45596..48790
                     /locus_tag="ACG90W_000332"
     CDS             45596..48790
                     /locus_tag="ACG90W_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0476728.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48936..49643)
                     /locus_tag="ACG90W_000333"
     CDS             complement(48936..49643)
                     /locus_tag="ACG90W_000333"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0476729.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16267 a         8794 c        10422 g        14442 t
ORIGIN      
        1 gtcacaatct ttttttcgaa taaacttatt ttttgaagtc ggccatttat cgtaatttct
       61 ttgcataata cgaacaaatt ttcggttatt gatgaaaaaa aacattgaaa atggtatgat
      121 aaaagaaaaa aaggaggaaa tttttaatgg tgaaatttat ccatgcggca gatttacata
      181 tggatcggtc atttgaagga ctgacaactc ttgataaaac tgtacaggaa aaactattga
      241 agacgaatct gaccgtttta tcaaatatca ttgaacaggc aataataaat aacgttgatt
      301 ttgttttact tgcaggagat aattttcatc aaaatcgtcc ttcattaaaa atacagaaac
      361 atttttcaga acagatgaaa cggttagaaa agaaccatat acctgtattt ataatattcg
      421 gaaatcatga tttctatcaa aaagagcggt attggttctc ctttcctaaa aatgtccatt
      481 tatttacatc cgaaacggta gagacaaaaa aaataacgat aaaaagtgga gaaactgtct
      541 ctctcagtgg atttagttat cgtcaaccgt ggattcaaaa agacaaagtg atggaatttc
      601 cttctagaga actcacagat taccatattg ggttgtacca cggggaacct ggcgtctctc
      661 aaaaaggaaa ctatgctcct ttccaaccct caaagatgca agaaaaggga tacgattatt
      721 gggctttagg gcatatccat gtgccaacgg tcttaaatga aaagcaaaca attgtttatc
      781 caggtgcgcc tcaaggtcat actaaaaagg aacaagcttc tacatctatt ctactagttg
      841 aattaagtaa aggaagttgc caaatacatc cgattaaagt agcagaggtc tattggaaga
      901 caaaggaaat atcattgagg aaagcaagaa cgacaaaaga ggtcattgtt cacattcgtc
      961 agcagctttc aaaagtagag gatggattta gtttaattga gataaaatta aaagattatg
     1021 accatctcaa tacagatgtt ttggaacgga tacaatcagg agagctatta gactatttgt
     1081 tagaggaatt ctcagatagg ataaatgatt tctttatttg gcgtatttct ctcatagaga
     1141 atacttttta tacgaagacc ccacttgctg cttctgctaa attgatggaa cagttatttc
     1201 aatactatca gacacctcaa gatttccaac aaattttaaa tgaagtatat agtcatcaag
     1261 aagcggctag aatattgagc gaactgccgg aatatcaaga agaaacactt gaaaaagcca
     1321 agcagctgct taatcaagat tttttatttg aggaggatca ggaatgaaga ttttaaggat
     1381 cgagattgct gcttttggaa aatggcgtca aaaaagtttt gacttttatt caggcaatca
     1441 gctgatctat gggggaaacg aagcaggaaa atcaacgatt tatcaattta ttcaagcaat
     1501 attatttggt ttcccttcaa aaggccgtaa aaaaaaggat tatacaccaa aagacggctc
     1561 agcctatggc ggcaaaatat ggcttaaaca tcctgtatat ggtgaatttg ctgtcgagcg
     1621 ctacaagcag caaaatcgcg gaaaatcgaa ggtgtggctg ggggatcaag tgggaagtga
     1681 tgagttacta gaaaaactga tcgcaccttt gacgaaagag ttattccagc aggtatttac
     1741 tttccagcaa gagcagttga tggaactaga tcatttgaga gaaaaagagc tacatgatgc
     1801 gctgatttct ttaggcatca cgggcagttc tcaagtattc caaaagcggc aagaatatta
     1861 tcagaaagca caacaattat tcaagaaaaa aggccaaaaa ttactaatca atcaaaaatt
     1921 aaatgaatgg aaagagctgc aagaaaaaat tcatcaaaaa caagaacagg aagcttattt
     1981 tggcaagttg atccaacaag aacaggaata cgagtcgcaa cagcagcagt tattggcaca
     2041 attaaaagag gcgaacaaac aattttcatt agccagacag caagaactta atttttcttt
     2101 atatgaagaa tggcaatctc tcaaaaaaaa cagcactaca ggtcttcctg atgagcaact
     2161 tcttttagat ctggagtctt tctccaaacg gtatcagcaa acaaatgagc gtttggaaga
     2221 attagaagca acactggaaa agcatagcgg gatggatcag cagtcagcca gatattattt
     2281 ttatttagag caggagcagg aactaaaaga tctccagcaa cttcaattta cagcggaaca
     2341 attaaacgaa gaatggcaaa gattacaaat tgagactaga gaaacaaatt cagaattgaa
     2401 gattttggaa caaaggtggc attggcagaa aaatccgtta ccggaagaat ttttatatga
     2461 agaagaatgg aagcatcttc tggctgaaga aagaaggatt gctgatctag aaagccagat
     2521 ttctgtgcgt ctccaaatag ctaaagaaca gcaagagatg attgaagcag aagtgaatga
     2581 cttggaagag acctatccag cattattaca aaatcagtca gagatagcac cagtctcttc
     2641 atctaaaccg tatatgctga tggctggcgg agcaataatc attgcaggta tatttgtacc
     2701 aatgcccttg aaagtgatcc ttttactaat tggtgcaggc atgattggct atttcttcta
     2761 caaaaggcaa ccaaaaatta aaactaaaaa tgatttctct gagataaaag gaatgtggca
     2821 agaaaaacta ggacaacttg atctagcctt agctcaatta gctgaagcag aagaaaaaca
     2881 aaagagaact ggagaagaaa aacagattct tcagcaagaa gtatatcgtt ttgcggatca
     2941 tcggcactta ggaaagatgg atacgctcaa actcatgaaa gaatatggac aggaagtcat
     3001 agattatcag aaattatatg gcgtatttga gcggaaacat aaaagagaac aggaaataca
     3061 agaaaaacta cgacaaatcg accaaaaatt tgagtttgtt aaagaatggt taccgataca
     3121 agataagaca atttcagaaa aaatggtatt gctttcccgt tttatacaag agatggaaca
     3181 gatgaaattt gccagaagct atcagcaaaa tactttattg aagcaagaaa tcaatcaatt
     3241 gaaaaagaaa caaaaagtat ggatacaaga atacagcgaa caattgactc aagcaggctt
     3301 atcttatcct tcggaagtac ctgtcttttt acaaaaagcc aaacaagccg aacagcagat
     3361 gaaaagatac aatgaattat cggaaatgct tactccacta tttcctgaag agctgacaaa
     3421 agagaaatta tctgtaagac tgcgccaact gaaagaaaag caatcccaac tccagcaaga
     3481 agaaaaagaa gcaagtgaaa atcggcaacg actccaactt caagtagaag agttgcaaag
     3541 agacggcaca ttggacgaac tttatcaaca agctagccgg caaaaagcaa aattacagga
     3601 attactggag gaatggggag cacttgaaat cgccgggaca ttcttaggtg atctttcaac
     3661 agagatgagt gaaagacaat taccccagtt gttaagagca gcaggtgatt atttaggtat
     3721 tttaacaaaa ggaaattatc agaagatatt actgaaaaat gatacactga ctttaacaga
     3781 cggatataca gaatttccca tctatgagtt atctacaggt acgaaagatc agctgattat
     3841 ggcgatgaga tttgcttacc tctcattaga agctaaccgt tcgatatgcc cgatcatcat
     3901 tgatgatggc tggcttcatt atgatcatca gcgaaagtat caattagctg aacttttagc
     3961 taaatttgga gaaaagtatc aaataatttg tttttcttct gataaagaaa tggtaagcta
     4021 ttaccatgag atgaaccaaa tcgtaaaaca attagaagga gcaaataatg aaaaaaattc
     4081 gtgatttagt aatcgatgag cagtttgaac aatttgtcct gatcaaaagt gcagatgtaa
     4141 gaatcgctaa aaacggaaaa aaatttattg cttttacttt ccaagatact tcaggtacta
     4201 tcgatggtaa atactgggat gcttcggagg aggaaatcaa taaatttaca gccggcacag
     4261 tcgtttttct aaacggtaaa agagaggttt atcaaggaaa ccctcaagta aagattcttc
     4321 atatgcgctt aacaagggta gatgagccag gagagcctag tttatacatg gaaagagccc
     4381 ctttgaagaa agaagaaatg gaagaagaaa tcaatcaatt gttgtttgag atcaccaatg
     4441 cgcattggaa tcgtattgtt cgctatctac ttaataaata tcaaaaacaa ttttttgagt
     4501 ttccggcagc aaaaagaaac catcatgcat ttgccagtgg attagcttat catactttga
     4561 caatgcttca cttagggaaa tcaatcgccg atgaatatcc tgagctgaat cgagcattgt
     4621 tgtatgctgg tatcattttg catgatttag gaaaggtgat tgaactttcc ggtcctatgt
     4681 cgacagaata tacgcttgca ggaaatttga tcggtcatct ggtattgatt gacgaagaaa
     4741 taactaaagc ctgtcttgcg ttgaaaatca gcgaagctga cgaagatgtt ctcgtattac
     4801 gtcatatggt tttagcacac catggcttat tagaatatgg ctcacctgta cgtccaagaa
     4861 taatggaagc agaaatatta catcaaatcg acaatatcga tgcttcgatg cagatgatgt
     4921 tgacttcgat tcgtcaaaca gaaccaggaa aatacaccga tcggattttc ggtatggaca
     4981 accgaagttt ctatgtacca aaagatttat agcaaaaatt tatttttttc attcaaaaca
     5041 aaaaaagcga gacataagtc ttcgcttttt ttgttttctt ttattttgat gaatcagttg
     5101 tttcttcagt tgaagattct tttgttgaat cagttgattt tgtgtcagaa ctctttgttg
     5161 aagctgtagt ttcacttgag tctttcgtac ttgatgaagt tgttgtgaat gcactcaaca
     5221 cattttcaaa ggcatcatct ttgattttta catttgcatc ttttaattct tcaccgatta
     5281 ctttagttgt gaatgcattg tcacttagtt tagtttcagt agcgatatct tttagttgat
     5341 ctttgtattt atccatgtca ttgcctttat tttggttttt caccattttg acaacgtagt
     5401 attctgtcgc ataagaagta ggatttgttg tagtaataac atcggagatc tcaccgtctt
     5461 tcaatttgaa agctgcttct tttacttctg caggaatagt tgttgtcgtt gagtcgaatt
     5521 taactttacc gccatcttct tttgtctctg tatcagttga tttgtctttg gctaatttag
     5581 agaaatcatc tccatcatcc gctgattttt tcacatcttt tgcttcatct tcacttgata
     5641 atttgatgat ttgtgcttct acttctggat ggaaagattt ccaagcagtt ttcaaatcgt
     5701 catctgtaat atctacgtga gctttcaagc cagcttcaaa agctaaattg ttacgaatat
     5761 attctttata agtatctttc gtatatccag cagcttctaa ttgtgattca aaagtatcac
     5821 ctaatgattt cgcttgtttg tcgtattctg catccacttg cttatcggtt actttatcgc
     5881 catatttgtt attgaatact ttataaatga tcatccgttg tactaaggat tggttcgatg
     5941 attcaagttt tgcttcatca tagaaatcag atacagtgat cgttccgcct ttcatcgttg
     6001 cgatatcttt attagtatcg cctgaacaag ctgctagagt taaaacagcc aacgcactag
     6061 tagctgccaa aataattgat ttttttttca ttaaaatttg cactcctgta attagttatt
     6121 atttttatat attaactata ccatatccca tttcctttaa aaacgttgtc gtggtagttt
     6181 caaacaaaat tcaaaatttc atcacattcc taggaaatac gcaaactttt ctcacttata
     6241 tagttaaatc tgttgagaaa ataatcatta ttatatttgt tcaggatact ctggcagatc
     6301 agcttttagt ttttctactt gttcattgat ttgtgtgata cgtggttcgg cttggaattt
     6361 aaaatcttcg atatcttttt tgattgcttc ttggaaagca ggaatcacag attcagctgt
     6421 ttcttttgtt tcgatcaatg cgcttttaaa atgattcagg ctgttattta aatcatttgt
     6481 cagagttgtt gcttcttcca attcttcgat cagtttcttt cttgtttcat tcccgctcct
     6541 gggcgccaat aacagacctc cgacagtgcc agcagcagct ccaaataaca gtcctttgaa
     6601 aaagttttta atcatcgcag gacacctgct ttacgattgt ttcttgaata gcttttagtt
     6661 cttccgatcc ataactatct tgatgattga caaagtgaat agaaaaatca tcctctttgc
     6721 tgtagcgagg gactaaatgg atatgtgaat gaaaaacaga ttgataggct agttcacggt
     6781 tgttattcaa gatattaaga cctttcattt ctggaaaagc tttttccaat gcttgagcaa
     6841 tcttaggcac acgcgaaaaa acctctcctg caagttcggg atcatattca aaaatatctg
     6901 ctacatgacg tttaggcaca actaatgtgt gtcctttcgt tgcttgagag atgtctaaaa
     6961 aagcatagac cttgtcgtct tcatatattt tatagcttgg gatttcttga ttgatgattt
     7021 tgcaaaaaat acagttttcc atataaaaca tcctttctat aaagaataaa tacttcagct
     7081 taactttacc ataagttaaa gctattgtaa ggaaaaaaac aaaataccgt cattttcttc
     7141 gtggtataat aaataaaaat aaatggcgga ggaaaaagaa tgagtttaat tatcgatcat
     7201 gtcacagggg ggtacggaca tctccctgtg ttaaaagata tcagtttcga tgtacagcct
     7261 ggagagatag tagggttgat tggactgaac ggagcaggga aaagtacgac catcaagcac
     7321 attatcggtc tgctggaagc ttcaaaagga acgatagaaa tcaatcatct atccttaaag
     7381 aaaaatacgg aagaatatcg caaacaaatt gggtatattc cagagactcc ttctttatat
     7441 gaagaattga cattgagaga acatatcgaa gtcactgcga tggcctacga tatacctatg
     7501 gatatagcaa tgaaaagagc agaaaagttg ttggagactt ttcggttatc taatcgtctt
     7561 gactggttcc cagctaattt ttcaaaggga atgaaacaga aagtgatgat cttatgcgcc
     7621 tttttgatcg agccttcttt atatatcatc gacgaaccat ttttaggctt ggatccacta
     7681 gctatcaatg cattgcttga attgatggta gaaatgaaaa aagaaggagc cggtattttg
     7741 atgtctacgc atattttagc tactgcagaa aaatattgtg atcgttttgt ggtcttacat
     7801 aacggagaga tacgagcaga cggttctctt gagatgttga gaaaagaatt tcagctgccg
     7861 gattcttctt tggacgaaat ctatattgct ttaacaaagg agcaggcaca tgagtagttt
     7921 ttttcaaaaa cgtttagcaa gacatcaaaa gaaaatgatg aaatatatgc gttatgtatt
     7981 gaacgatcat tttgttttag tttgtctttt cttgattggc ggtgtgggat tatattattc
     8041 taattggttg aaaaccctgc agccaccgtt tgcaattgga gggctcataa tctctgtatt
     8101 ttggatgctc tgtttattcg tgggtaagat ggctactttt gcagaggcag cagatattgt
     8161 ttttttactt cctaaagaaa aagaaatgca aacctatttg gaacgagctt ttcgctattc
     8221 ttgcattttt ccgttgatct ttttgatatt ggcatgcgga ttggccatgc cattagtggt
     8281 ggttagtacg gagcaatcct tttcgatgtt tttcgtctat ctgatcatat tatggagttt
     8341 gaaattcagt catctgcaaa tcaagagagc agaattgttt cgactagatc aacaagttat
     8401 tcgttattgg aaattaggct ggtttttttc ttctctgatc tttttgtttc tatcatttta
     8461 ttggctaata tgggcaggaa tagcaggtgc attagtacag gcaagtctat attactggtt
     8521 tttgtggaag caacaagaaa aaggattgga ttgggagaaa atggtgcatg tagaagaaca
     8581 taggttgcat cagatctatc aatttatcaa tttattcaca gatgttcctg aaattacggc
     8641 aaagatcaaa agaagacgct atttggatcc tatattgact catattaaaa gagaatcaaa
     8701 aaacacttat ctttacttgt ttgcaagaag attggcaaga ggttctgatt ttagtggtct
     8761 ttatttccga ttagtaatta taggtgggtt attgattgcc ggagtagtcg acttttattt
     8821 tagtctgatt atcggtttct tatttttata tttgatcggt ttccaactgt tacctttagc
     8881 gaaccagttc cgctatatgg ttctcgttca gctttatcca atccataaga atcagaagaa
     8941 gcaggcgatt cagtatctac tcacatgggt attaattatc gcgtcggtta tttttggttt
     9001 gattgctgca gtcgtattag atgggcagca gcgatgggtt ccgctagcgg cttatttgat
     9061 tgttactatg ctctttgtta aactatatgt accaactcgt ttgaagaaga tgtcagactg
     9121 aaaacaaagc tgtgtagggt aaaaatgaga atgaaaaata gagcgttgac agtaagaatg
     9181 ctgttcgttc tatttttttt gagaaaagct ggttttttga atggcaatta aatgaaaaag
     9241 aacgtatatg tgcttgacgt gtagtgttca ggcgactaga ataataagta ctgcaaaaca
     9301 aagagtaaga ggacgggaga atgcatgatg gagtttcagt tagaccaaga gtggcggtta
     9361 cgtcccatca aaggcgacac aggaaaaaca tacataggca tgcgtgatga ggacaaagtc
     9421 tttattaaac gaaacacgac tccgatgttg gctgctcttt caagggaagg aattacacca
     9481 aaacttgttt ggacaaaacg aacggggaat ggcgatactt tgacagcaca agagtggctg
     9541 gaaggtcgct tgctgactcc taatgagatt ggtcaaagaa acgacgtgat tgatgttttg
     9601 taccatttac atcattcaaa ttctcttaaa accatgctga cccgtatcgg tggtcgtgtc
     9661 atgacgccgg agaagatgct gcaaaaatat gaaaaagaat taccggaaga actccagaaa
     9721 aatcaatttc tgcaaattgt ccatcgtttt cttcgtgagc agatcccgtc aatagataag
     9781 gaaaacttat cagtagtaca cggagatgta aaccatcgta attggttagt atgtaaaaat
     9841 tatttatatc tagtcgattg ggattcaatc atgtttgcag accctgcggt ggatattgca
     9901 acgattttag gaaattatgt gccactgtct agctggaatc agtggctgat cagttatggc
     9961 atacgcccga ctgacgaaat gcttgaaaaa tttcattggt atgcggccat gaatttgctc
    10021 caagaaatca ccagatattg caaacgtgaa gatcatcgac ggatgaacga agaaatttta
    10081 caattaaaac gaattttcag tggctgaggt ttggcccaaa ctctgcggag atttggccag
    10141 acctttttaa ttagaggaaa ggaatggaga taaataatgc gtgtccgaaa tcgtccggga
    10201 gcagcggaaa tgttagcggc tcacccgaat tttgtaatta gtgatccaac attgtggaag
    10261 ggaaaatgga atgaattgtt tgaaaacgat catccaatcc acattgagat tggtatgggg
    10321 aaaggccagt tcatcactgg aatggcaaaa gcccatcctg aaatcaatta tattggagta
    10381 gaaatgcagg tcagtgttgt ttcgattgca ctagacaagt tgatcgaaca accgcttcct
    10441 aacctgaaac ttctacatgt ggatggatca gcgttgacag agtatttcgc tgacagcgaa
    10501 gtagatcaaa tctatctgaa tttttccgat ccatggccaa aaaaacgtca tgaaaaacgg
    10561 cgattgacat ataagacttt tcttgctgta gatgaacaga ttcttcgtcc aaatggagaa
    10621 atccatttca aaacagataa tcaaggattg tttgagtatt ctctggcaag tttttctcaa
    10681 tatggcatga tcctcaaaca ggtatggtta gatttacatc aaagtcaatt tgaaggcaat
    10741 attatgacgg agtatgaaga aaaattttct tcaaaaggtc aacgtattta tcgagtagaa
    10801 gctcaatttc aagataagac caaaaagaaa agtctgtgaa actaactttc tgctttcata
    10861 aaaacattaa tatccataaa taaatcccag aaaatgaaca ttacagatgt aatgtatttt
    10921 ctgggattta tttgatttaa aatgtattta tttcatcggt ttttccacaa ggtggaatca
    10981 attggaagcg agaaaatgaa aataatcctt tgtcggtgat cttcaattcg ggtatgacag
    11041 ataatgttaa gaaagaaagt gtcaaaaaag gatcaaaggt tttagctgct cctagttgat
    11101 gtgctttagt tattaattca ttcaactgtt catagacgat cgaaaaaggt tcctgtgtca
    11161 tgattccgcc tataggaaga gaaacacaag ctatttcctg ctgtttgttg acaacggtca
    11221 tcccgccacc tttttgaatc aaaagattag cagcatacag catatcctcg tcgtttgtcc
    11281 caacgacaac tagattatgc gagtcatggg caatcgttgt cgctatcgct ccttgtttca
    11341 gctgaaatcc tttcactatg cctaatccaa tgtttcccgt ttgatggtga cgctcgatca
    11401 ccgcaatctt taattggtct ttttcgattg aaggaatgaa tcgttcgcct tgtcggtcca
    11461 ctttttctac caaatcttta gtcactagac tattcgggat aatttcgatc acatgggctt
    11521 gtgaagaaga aagaagcagc tctaacgaat gtggatttaa tggttgagcg tttagttttg
    11581 gcagacaaca cgtatatgaa tttgaatgat cagcaaataa atggtcttgg agttgcccag
    11641 attgaacaac taatgttccc ttatagaaaa cttcatgaat tggcagcttg gtaagatcgt
    11701 ctgttaagaa gaaatcagct tgatacccag gggcaatcgc acctagtgtt ttcaaatcaa
    11761 aacattctgc tgcattcaat gttgccatct gaatggcttg taatggatcg attccaagtg
    11821 caatagcttg acggacgata tgatcgattg atccttcttt tactaaatca ttgatcagtt
    11881 tatcatcggt gacaaagaag catcttcttg aattttcagg tgtgatcgcc ggtagtagcg
    11941 atgccagatc ttttgcaacg gtcccttctc gaaccattaa atacatacca agatccaaac
    12001 gttctttagc ttcttttgca gttgtagctt catgatccgt ccgaatacct gctgcaggat
    12061 aaacatttaa ctcttccatg ccgataccag ctgcatgccc gtcaattttt ttcttatgct
    12121 ggtgcatcaa ttggatcttt tctaggatgt ctgtttcatt atttgctact gcttgatagt
    12181 tcatgacttc agctaagcct aaaacttcgg gtttttgtaa aaaaggggct aatttttttg
    12241 catttaaatt tgcaccattg gtctcaaaag gggttacagg tacacaagaa ggtagcataa
    12301 aaaagatatt catgagtgtt tgtcttgcat cttctatcat aaactcaaga cctgtactcc
    12361 cagcaatgtt tcctatttca tggggatctg tcactacagt agtaactcca tgaagcaagg
    12421 atacttttga aaattcgctt ggtgataaaa gtgagctttc gatatgcaca tggccatcga
    12481 tgaatcctgg cactagatat tgcccattag cattatgtac gctttttcct tcgtattcac
    12541 cgattcctac gatcattcct tcgcagattg ctacatcttt ttccatgatt tctttcgtaa
    12601 agacgttgac gatcttagca tgcttgatca ctaggtcagc aggctttttt ttacttgcta
    12661 cttcgatata tttccttaat gttgtttcca atggagtgcc tcccaagata aatttttata
    12721 agtataacat aaattcggtt tttgccaata tttttttgta aattcatttt atttgaaaca
    12781 aaaagatcaa aaagacgtat gttttttgtg gaggggagaa taatggaaat aaaacgatta
    12841 tcggatcaaa tgataagtga aagttttgcc aaacatgccc aagacgtata tatattgcca
    12901 gcttcagaag tattttacat ctattttcgc tatggaaaaa aacgaacatt ctggaagaaa
    12961 gtctcttcgg cagatggaca gcaactgatc tcaagattta agtatttggg acagatggat
    13021 atcggagaaa aaagaaaagc tcagttaggt gcatttactt attctttaga aaaacaggaa
    13081 gttcgtctgc ggttgtcttc tgtaggagat taccttcaac gagagagttt agtgattcgg
    13141 ctattatacg gtacttttga aacaccccat cgttgcttta gttctcgaga ttttgcaact
    13201 attttacaga aaaccgtcag acgtggatta taccttttca gcggaccagt cggatcaggc
    13261 aagacttctc taatgtatcg gttggctttg gaagaaaaat tgcaagtgat tactatcgaa
    13321 gatcctgtag agatagaaga accgtttttt ctgcagctac agatcaatcc gaaaatagca
    13381 cagacttatg atacactttt gagactcgca ttacggcatc gtccagactt gctaatcatt
    13441 ggcgagataa gagatgaatc aactgcacat gctgcggtaa gagcagcact aacagggcac
    13501 cgggtttttg caaccgtaca tgcacgcaat cttgaaggta cattggtcag aatccatgaa
    13561 ctgacaaaga aaaaagaaga gctatctgaa tgtttagctg gtgtcattta tcaagaactt
    13621 ttggaagatg ccaatcacca tccttctgtt ttatggagtt atcaatttat ggaggaagaa
    13681 tcagtaaaaa aatcatggga ggaaagttat gaagaagctc aggaaaatat ttggcagtca
    13741 gaactattct aaaggattaa caagaagcca gcaaaaccag tttgcacaaa ccatgggaga
    13801 actgctttta tctggtttta gtctgcaaga atccctttat ttattactca cgatccgttc
    13861 gtttccacct gttattttgg caaatatcca acatcagcta aaggaagggc aagcgtttag
    13921 tgaggtactt tcgcaaatag gatatggagc agagcaactg gtacagatcg agttagctga
    13981 acgtcatggg aatctgccga caactttgtt ggaaattgcc aaacagtttc ggcttgttga
    14041 aggatttcgc aatgatttga aaaagctgct tgcatatccg atgctattgc tcaccttttt
    14101 gctaggtatc ttgtttagta tgcggttgtt cgttttgcct cagcttattg gttcaggaat
    14161 ggtcatgcaa gagcattggg gtattcagct gatacaagtc tttcattggt atgttctact
    14221 attcatccta gcggttactc tattggcttt actagtaagg tatcgtctga tgaagttgtc
    14281 tcctgttgat cagagtgaat ggataagcag acaattattg attggaccga tatattcctt
    14341 gtatcaatcg gcttatttgt cactagagat tggaaagctt tttcatgaag gtttcgaatt
    14401 aaggcaaatc atagtctgct taaaggaaac gaaacaagaa agtctgattc agcatcttgc
    14461 ctctaagttg attcaaggat tggaggcagg tatgtcattg gctgagcagt tccagaatta
    14521 tcgttttcta tcagaagaat tcagtaaaat tgttttacag ggagaggcaa agggaagttt
    14581 aggtaaagaa ctgatttttt acagcgaatg gacaagaaaa cagcttttca aacgtcttca
    14641 tcactggatg cattttattc agccaatcat ttttttgggc gtagcggctt tgattttatt
    14701 agtttatgcc gctgttttat tacctgttta cggaagcata gaggaggttt tgccatgaaa
    14761 aagagaaaaa tatcttatta tagcggtttt acattgatcg aaatgctaat cgtcttactg
    14821 atcatcagtg tattagtgtt gttgttcgtc cctaatttgt cgcgttatag gaatcatgta
    14881 gaccaagaaa gtcgtgaagc gatcatccag ttggtagaca cacaaaaaga gctctatgcg
    14941 cttcaaaata atggaagagt tcctactgtg gaggaattgt tgaatgaagg ctatatcaaa
    15001 cgagaacatg cagaaatcta tcagcggcct tgaaggtaag gttttagcat caggatatac
    15061 gttgtttgaa tcactcttac tgattttatt acttacaacg atcctttctt ttccagttct
    15121 cgcattttct gcatggaaga atgaattggc agtctatcaa tttttcggcc aatttgaaaa
    15181 acgaatctat tctacgcaaa aaatcgctat tgtgaatcag atccaaacag gcttttattg
    15241 gaatagtgaa gagaatcaaa tcatttttca tgttccgaat ccagataaaa taaattggga
    15301 agtattagat attcctgatg agattacctt gaaacgccat gcttctatta cttttgctgc
    15361 tagaaccggg aatgaaagtt cactaaaagc ttatcaattt tattgggaag aaaaagagcg
    15421 gacgattacg tatcaattcc agatagggag tggtcgctat acaaaaagaa ttgaataaaa
    15481 caggatttgt tttgttggag tcccttacag cgcttatgat cgcttctgtc tgtgtctact
    15541 tagtgagctt tggccagctg tttttgcttc aggcagaaaa gaaaagtggg gaggaactgc
    15601 tatttattcg aatgatttat gaagatatca agacgcagcg gatgtatcac aaaagaccag
    15661 agcgaaggga agaaatgatc ccatacgata tccaaattgt cgataataat ggagtaatcg
    15721 aaaaagtgtt tgtcaatatg gagaaagaag cgatcagtat tgaaaaaaca agtaattcca
    15781 gcatttacgc tgattgaatg tctaattgga ctgatgatat tgagcagtct attgctcaca
    15841 ttcaatcttt tgattcagca aaccatccaa gtaagtgaat ttttgcagcg taaagatcaa
    15901 aaagagtggc taatattttt agcacaatta gaagaagaac tcaagagcag tcacaatgtc
    15961 tctgtgaaaa atcagcaact gcactacacg atcgaggata aacagtatat catcgaaaga
    16021 tatcaggcaa tgatacgtaa aagaagaact tccggcggac atcaacctat gttaacaagt
    16081 atcactgagc ttcaattatt ggaaaaagaa cacacaattt ctttttatgt acactttgaa
    16141 aatggagaag atggatatgc catatggaca aaaaacgatc agtagaaaat gttataaggg
    16201 ctctatttta ttttctgtct tgcttttatt tcttttatgt acgtttttat tgatggcagc
    16261 attagaaagc taccgtttgt caatggatct taatatacga atcaaaaatt attatatagc
    16321 aaaaataatg aagcagatgg ctttatcgga acagtctacg cttgccgaaa gttcggaagg
    16381 agttttttac tacacaactg gatctgtaac atatcagtgg gtacaacagt ccctattctt
    16441 agaggtggaa gtctcaccgt ttatctttcg gttcacagaa gaagtaaaaa atgatacaga
    16501 tactagtaca gaatgatgga atgttcgtga aaacgccctc ttttctgtta taacactgtt
    16561 tttaataaaa aaatgtttgc cgtttcacaa ataaaaaaca gtgaaaacga ttgaaatttt
    16621 gtcaattgtt cgatagaatt aaacaggtga aaaaattgtt ataatatatc tagaaatgag
    16681 gtgcaccatg ttaccagaaa aaatggaaca agggtttaat caaaacctgg aagctattca
    16741 gcttttacag aatgcattgg gaacttcctt tttagaagcc tatgtcgaaa atgcagaaaa
    16801 tttaatagat gattatcaag tacgtgtggt ggatggagtg ccgacaaaag agacaacgca
    16861 acggatcaca gctctttatg aagagttgaa gaaactttct ttcgagccag aagaatggcg
    16921 cagactgtct caattacttt tattgaaggg cagccagaca gaacatttgc aggcgaacca
    16981 tcaactcact cctgatagta tcggtttttt atttgttttt cttattgaac aactatacac
    17041 aaataaaaaa gcaccggtta agattctgga tattgcggca ggaatgggca acttgttatt
    17101 aacagttctt ttaaacctat cgaatgcggg gtaccaaaca gaaggaattg gtgtggatat
    17161 tgatgataca ttgttggctg tcgcagcttc tacaagtgat ttgacacaag caaatgttca
    17221 atactttcat caagacgggt tgcaggaatt attaatagat cctgtcgatt ttgcaatcag
    17281 cgatttgcca atcggttatt atccgaatga cgaaaaagca aaagagtttt taacaagtac
    17341 cgaagaaggc catagttatg cacatcattt attgctagag caatcaatga aatatgtgaa
    17401 gccggatggt tttggtttgt ttttgatgcc gagtggattt ttagaaacgg atcaaagtga
    17461 agaaatcaaa aaatggttta aagaagaagg ttatcttcaa ggaatgattc aattgccaga
    17521 tgaattgttc cgtaataaac aatcgcaaaa aagtattttg atcttacaga aaaaaggacc
    17581 acaagcacaa caagtaaaag aagtcttgct tgtgaaactt gcttcattaa aagaaccaga
    17641 gaaggtgacg gaatttttca acgagtttaa aaactggaaa tcatctgctc tgtaatcatt
    17701 caacgagaag gagagtcata tgtctaaaac aattgcaatc aatgctggga gttcaagttt
    17761 gaaatggcaa ttatatcaaa tgccagaaga aaccgtagtt gcgaaaggga tcgtcgaacg
    17821 tatcggttta caagattcta tttttaccat caagtacggc gataatcaaa aattcgaaca
    17881 aatcatggat atcgaaaacc acgaagtagc agtaaaaatg ctgttagatc agttgattga
    17941 actgcaaatt ttaagctctt atgatgaaat caccggagta gggcaccgtg ttgttcacgg
    18001 aggagaaact tatggggatt cagtagtgat cgatgagaaa gtaatggacc gcattgctga
    18061 attagcagaa tttgctcctt tgcataatcc tgcaaactta atggggatca aagcgtttaa
    18121 aaagatttta cctgacattt tgagtgtggc tgtattcgat acatctttcc actcaacaat
    18181 gccaaagcaa aattatttat atagcttacc ggtgtcttat tatgaagatt tcaaagtaag
    18241 aaaatatggc ttccatggca caagtcatcg ttatgtgtcg gaacgtgcgg caaaattatt
    18301 gaacaaaccg atcgaagaac taaaaatcat cacttgccac ttaggaaatg gtgtatcgat
    18361 cacagcagtc gatggaggta aatccatgga tacttctatg ggattcacac cattagctgg
    18421 ggtgacaatg ggtacacgtt caggtgatat cgatccagct gttctcccat atttaatgga
    18481 aaaattagag attgatattg atgaaatgat caatgttttg aacaagaaat ctggtctgct
    18541 aggcctaaca ggattgtcta gtgatatgcg tgatcttgaa aagaactatg aaaaagaaca
    18601 tattcgttta gcttatgaca ttttcgtgga ccgcatccga aaatacatcg gcggttatgt
    18661 gacaacgatg aatggtgttg atgcaattgt ctttactgct ggtatcggcg agaatgacgg
    18721 acatgtacgt agtgaaatca tcaaaggtat gtcttggttt ggttgtgaaa tcgatccagc
    18781 gttgaatgaa ttgcgtggtg aagaagtaga tatctctaca aaagaatcaa aagtaagagt
    18841 attagtgatc ccaacagatg aggaactaat gattgcccgt gatgtggagc gtctgcgcgg
    18901 ctaagccaaa gcattcgtta ggataaaaga tgaaacgcat gcaagaggga attcttgcat
    18961 gtgttttttt tgcggagaag accgcaaaat ggtaatttaa cttcaatttc ccttcttttt
    19021 tacaggatct tcttagggaa ttttgcttgg aaaagtggtc aaaagggaaa agactggcat
    19081 tttatttatt gattttcagg aaaatcccaa tattttattt tcattcatct ttataaggga
    19141 cattacatat gtaaaaagaa aaaacatttt tagatcagcc tcgtgggctg tcgactttat
    19201 tcttcgcttt atgtttagct agaaaaccga tccgaaacat tcattgatcg aataataaaa
    19261 acttgatttt ccacctgtac tttgcctgaa tcgtgaaagc acaggtatga aaatcaagtt
    19321 ttttgttgcg aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc
    19381 tcctgatcaa taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg
    19441 actgctagga gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag
    19501 ctgtcgaggt gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt
    19561 ttcagcaacg ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca
    19621 tcgctttgat aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc
    19681 aatgtgatgg cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc
    19741 aaataaccga tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca
    19801 ccgagtaata atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa
    19861 tgatctccac gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc
    19921 tggtgcaagt tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt
    19981 tgcagccgat ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt
    20041 tccagttctt tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga
    20101 aaacgacgaa tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt
    20161 gtcagctcag ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac
    20221 tcaatcaatg caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc
    20281 cgatcatgag aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag
    20341 cgaatactgt taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg
    20401 gctgctaaaa ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac
    20461 cgtgcttcct gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct
    20521 aatacgtaac cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac
    20581 ttctctaagt ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg
    20641 aggtcctgta tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact
    20701 gtaagttctt gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg
    20761 tcgacacctt ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata
    20821 ccgactaagg caattttttc accagcttca atttgacagg ataaatcctc aaataatgga
    20881 ttcccgccga ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag
    20941 accactcaaa aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga
    21001 gaaaaatacc ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg
    21061 tcccgtcaac agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt
    21121 aattttcatt acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag
    21181 ttgtcttttc cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta
    21241 atatttatcg atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct
    21301 cgtggaccac atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt
    21361 tgataaaaaa ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa
    21421 acagctcttc tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca
    21481 atggcaagcg gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt
    21541 ctcgcggtac ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat
    21601 cgatacttca tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata
    21661 agctttggca tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc
    21721 actttttttt attttacttg aactcattga ctgttttccc tttattttaa acattataaa
    21781 gaccgatgag tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct
    21841 gccgatagtt ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg
    21901 gttttctaaa tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc
    21961 agaaacagaa ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat
    22021 caaatctact tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata
    22081 ttctacgaca gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc
    22141 caatgtttgc tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac
    22201 ggtttggaaa gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat
    22261 agctacattg accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac
    22321 catgattttt ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac
    22381 tcttttattg tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat
    22441 tttttttgca aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac
    22501 caaggcaata aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc
    22561 atatgcagct ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac
    22621 aaaaaagata tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc
    22681 gataatcatt aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa
    22741 gcaatttgcc aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg
    22801 ttgtttgtcc tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct
    22861 tcgaactacc aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat
    22921 caataaaaat gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc
    22981 tctatagtag catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt
    23041 ttcttgtcta gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac
    23101 ccagagcctg ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt
    23161 tatctggtaa atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac
    23221 ccacgccacg gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa
    23281 catctccagc tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag
    23341 aattggattt tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct
    23401 caggcaatcc caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag
    23461 gattacctaa acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca
    23521 gatcacctgc ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac
    23581 gaatcgattt ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg
    23641 taagagcttt tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt
    23701 ctttatctga aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat
    23761 cgccgttcgt tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt
    23821 gtccaaggcc gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt
    23881 ctgttaatgg tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa
    23941 tataaggatt ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa
    24001 gcagaaaatc ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg
    24061 taccactcat ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg
    24121 cgtttagcat gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt
    24181 ttccagtacc aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc
    24241 ggcgtatgat ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc
    24301 gaggtctcat gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac
    24361 gtcccttaat tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg
    24421 ttttcatagc ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat
    24481 aaacaaaaga tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta
    24541 ttggggatgt atttggagca tgacacacca gaaggaagat tagctggata tattgtagac
    24601 gcggaagcat acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca
    24661 ccgcgtgtgc gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact
    24721 catcggattt tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt
    24781 gcaatcgaac ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt
    24841 ccagatatca gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt
    24901 tatggtcagt ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca
    24961 aaaaagatca atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag
    25021 ccgttgcgtt ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc
    25081 gagaaagatt ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga
    25141 tgaaataatt gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa
    25201 tcatacgatc gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga
    25261 tgatgcagaa gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct
    25321 ttttaatcct cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga
    25381 aggaaaaaaa gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt
    25441 attagctcaa gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt
    25501 attcgagctt cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa
    25561 tgaagcagat acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa
    25621 tgaagtaaaa gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga
    25681 ggcaggggcg agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga
    25741 tcaatatggt gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt
    25801 catggcttat tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg
    25861 tatcacacaa ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga
    25921 agtagaggaa agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac
    25981 gcgaatgctg acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa
    26041 aattatcaca ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt
    26101 gacattgaca gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac
    26161 tgggtcgggc gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt
    26221 tgatttagac gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc
    26281 caaagatgtc catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt
    26341 aatcgttgcg aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt
    26401 actgaagcaa acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt
    26461 tctggatgcc atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg
    26521 gtatgcaatt attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga
    26581 atcatatgaa tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg
    26641 ggttatgagg atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat
    26701 tgttgcagaa atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga
    26761 agaaaatcaa aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga
    26821 aaagctggat tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt
    26881 cccggcatca gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg
    26941 gcttgaaaag attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt
    27001 agagttattg gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt
    27061 ggttgcttat gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag
    27121 tgaaacaaaa ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg
    27181 cgatgaagtc gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat
    27241 ccttcgtaca gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt
    27301 aatgaatcct tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt
    27361 gaaaaaatag tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa
    27421 tcgtttgaaa aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga
    27481 ttttgggaca attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag
    27541 ggggatcgat tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg
    27601 ttagtcaata catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga
    27661 ctgctgcaca tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc
    27721 aagtcgcagg tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct
    27781 tacatgatgt tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg
    27841 atgacgttgc tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc
    27901 acgaagagca gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac
    27961 gagttatcat ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc
    28021 gagaagataa gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac
    28081 atcgtctagg gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa
    28141 atccaaaaca gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa
    28201 aatacgtaag tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg
    28261 cagaaatcta tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga
    28321 aaaagcagtt caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca
    28381 aggattgtta tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat
    28441 ttaaggatta tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga
    28501 tcggtccggc aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg
    28561 ctgaattcgg ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac
    28621 cagatggtat gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa
    28681 gctatgatgc ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct
    28741 acgtcttcac accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact
    28801 ttgcatacag tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca
    28861 aaatggtgca gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc
    28921 caaattcatt tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa
    28981 ataagatcaa acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg
    29041 aatccgtggt caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga
    29101 acaaactgca agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag
    29161 ctgtagggta tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac
    29221 gtaaagaaca aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga
    29281 aaaaagaacc tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag
    29341 aaaacttact aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg
    29401 gttatatcac taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc
    29461 ctgacaaacc aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata
    29521 cacgaaaaga gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga
    29581 atgatgtact tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga
    29641 cgaataaaga taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc
    29701 taaagagtat cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga
    29761 atggatagga ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca
    29821 gtcgatcagc aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat
    29881 gaaaccgata cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt
    29941 tttgaagatg aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta
    30001 agtatctctc aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg
    30061 gaagccgcac gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga
    30121 agtcgcggca tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc
    30181 aatgatggac ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc
    30241 actagtaaaa actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg
    30301 cttttttgtc tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa
    30361 ttatacaagt tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata
    30421 tcaaaatcac agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac
    30481 gccatgaagc ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga
    30541 tctacggatc atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg
    30601 ctatgccaaa attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg
    30661 gagctgctca tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg
    30721 aagattatca tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc
    30781 aatttgaact atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag
    30841 gtatcaattt atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct
    30901 ctatgatcag cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca
    30961 aaaaatcaga aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg
    31021 gaaaagaaaa tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt
    31081 tacctctgga agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa
    31141 ctgtgtgggc ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat
    31201 taaaaaatga cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc
    31261 ctttcttccc aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta
    31321 taccagaagg cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag
    31381 ttgaagcgcc agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct
    31441 ataaagaaga atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct
    31501 gtgcaggaga atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata
    31561 agtttgagtt ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt
    31621 atccgaatag ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa
    31681 ggaagatcaa acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat
    31741 tgatcaaaat ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa
    31801 atctaaagct tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac
    31861 agacactcgt ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta
    31921 tcttttagca atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc
    31981 ttggaaatct ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag
    32041 ttcatatttt cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt
    32101 gtttttttca agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt
    32161 atcttctact acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa
    32221 gtttgacaca ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac
    32281 tgattcattt tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc
    32341 agtttgcaga cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc
    32401 aaatgttcgc cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg
    32461 atcatattct ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta
    32521 agcataagcg gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt
    32581 tcctgtctca tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa
    32641 tccaagctgt tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc
    32701 ctcctttttg tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag
    32761 gggaaatagt cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat
    32821 cgaatgtcaa tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc
    32881 tgaaaatgat ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc
    32941 cgtcccgcgt acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta
    33001 ccgtgtatga atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca
    33061 tagctaagaa agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt
    33121 ttgccaggcg aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga
    33181 gattatcagt ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga
    33241 agtgtagggg atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg
    33301 gaacgccatg tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag
    33361 cataaattgt tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg
    33421 tttcgctacg aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa
    33481 gcatttggat cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc
    33541 ttcaaacagt tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa
    33601 acgcgtcaag cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg
    33661 agtgaagact ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac
    33721 aaacgagatc aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct
    33781 gctcaaaaac attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa
    33841 atcgacagta atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg
    33901 agtgacgcac caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat
    33961 ctagtggaag aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag
    34021 cgattactct tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat
    34081 gcttacgtgg tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg
    34141 attcgaaact ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa
    34201 tttaagacag cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt
    34261 gctagtcaaa cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta
    34321 gaagagatat atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag
    34381 gaaaaaaata aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag
    34441 aacaaacagt tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct
    34501 ttatcgacct acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca
    34561 aagaagcatg ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg
    34621 aagttagaag acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag
    34681 tgattgcaag tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag
    34741 atgatcaagc aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc
    34801 caaaaatgac taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt
    34861 tagatgataa tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag
    34921 gcgcaagaga ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc
    34981 aatctcctca attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa
    35041 tcgttcgttg tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag
    35101 tagatattga aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga
    35161 ttgcgaaggt aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga
    35221 cctatgatga agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg
    35281 aattgatcga tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc
    35341 ttgaaaacgg cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac
    35401 gaaaagacat ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat
    35461 ggctgaaagt agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa
    35521 cagaagcaat cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag
    35581 ataaaagtga aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat
    35641 taggactaat tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg
    35701 aattttcaga agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag
    35761 aagaagatat cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata
    35821 tcgtcttgaa cggttatgaa ttaggcggag gttctatccg tattcatact cgtgagcttc
    35881 aagaaaaagt actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt
    35941 tcttgttaac agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg
    36001 accgttttgc aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta
    36061 aaaacggaaa agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat
    36121 tgttcgaatt gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag
    36181 gatttgtcga atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga
    36241 agccataaca tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc
    36301 atgattacac gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat
    36361 tgaatttctt ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga
    36421 ttttgacgac tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt
    36481 atttactgaa tatacctttg tttgtattgg cgtggttaaa aatcgggaag aagtttacaa
    36541 ttttcacttt tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag
    36601 tcctatctac cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta
    36661 ttgggctagc gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga
    36721 ttcgtaaaaa aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg
    36781 tttttgttgc cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt
    36841 tcgtcagtgg aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga
    36901 tcgtaacgaa acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga
    36961 tcacgatcat caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga
    37021 cgatcgttac acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac
    37081 aagcttttgt aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca
    37141 tttagatcga gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct
    37201 gcagtttttt tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt
    37261 tcttaaagtt gaaaaaaagc atgtttattt ttttaaattt atgggatgat agaaaaaaat
    37321 agtagagaaa ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg
    37381 aaaaaagttt atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc
    37441 cgctacatca ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat
    37501 caccttctgc caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta
    37561 tctggttatt tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat
    37621 atttggcaat tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt
    37681 gtgacagctt cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga
    37741 gttgtggtca gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct
    37801 tatttcgatc gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa
    37861 ggtcaaaact atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa
    37921 agaaaatgga tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg
    37981 atcttgtata caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg
    38041 ttttctatat ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa
    38101 aacatcccga aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca
    38161 ctgattcttt gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg
    38221 ctcctagtga gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt
    38281 ttgaatcgat ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt
    38341 tatctgtatc agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat
    38401 catgttttgt tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct
    38461 tatcgatatg tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg
    38521 aaaggatggt tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca
    38581 ttacttgtag caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc
    38641 gatcaaaaac aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga
    38701 aacaattcag aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg
    38761 tccaaggcac aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt
    38821 atgttggatg ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat
    38881 gtcggacgac aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg
    38941 ttgaaagaca ctgttctgat tggactagga acgaatggct cattctcgga agctcagttt
    39001 gatagtgtga tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg
    39061 acgaagcgct ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac
    39121 aatatgacgc tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat
    39181 gatcaagtcc atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc
    39241 ttgctaggag gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta
    39301 caaaaaactt caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt
    39361 tttaacattc atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct
    39421 ttttgttttc gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa
    39481 gaaaatactt ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta
    39541 gcaccagtac cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca
    39601 taaaatgtct gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat
    39661 ctcacgaatc tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac
    39721 tttgaatccg tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt
    39781 tccaggtttt gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac
    39841 ctgaatgatt tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt
    39901 tagtagctta cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc
    39961 gattgccagg tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg
    40021 attacagacg actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc
    40081 gatggtgtgg acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat
    40141 gtctgctaga cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa
    40201 aaaaagtcca actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac
    40261 acgggaaaag tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg
    40321 gataatcttg atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga
    40381 ttccctccaa aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca
    40441 aggtggcttg tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag
    40501 agactatgtt ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg
    40561 gaaatgcccg ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg
    40621 ctccccatcg tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat
    40681 tcagctttat ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc
    40741 gatccaatgc ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg
    40801 taaagatttt gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg
    40861 ctacagaact taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc
    40921 cagctgtata tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag
    40981 tgcgtttcgg cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg
    41041 cggagatcga acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg
    41101 atttcaaata tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg
    41161 aatctactgt cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc
    41221 tgccctcttt ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa
    41281 gtgttataat aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc
    41341 cgattggcat atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt
    41401 actatcttct atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg
    41461 agacttatat gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat
    41521 ggtcgattgg aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag
    41581 cagtacgaga ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac
    41641 acagctagca gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc
    41701 ttactttgaa ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa
    41761 gcaagcggta gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca
    41821 agtattgatt agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat
    41881 cgaagtaggc gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc
    41941 actaggacat ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc
    42001 cccattgaaa ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct
    42061 gacaacaggc ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat
    42121 aacagcgagt ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa
    42181 ttacatctat atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg
    42241 gcaaatatat cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa
    42301 gcgcaaacga aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag
    42361 tgaaatgacg gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca
    42421 agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt
    42481 tggtcctttt atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt
    42541 gatcagcggg aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact
    42601 ttttggtgaa acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac
    42661 accagacgaa gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat
    42721 cgaacgaaaa ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc
    42781 agccaaagta acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa
    42841 acgtacagaa gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc
    42901 tcaaatcgtt ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga
    42961 taaagagact gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg
    43021 attaaaagaa aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca
    43081 tcttctgcaa aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc
    43141 cgttttagcc caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa
    43201 agaagctcta acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt
    43261 tgaaagcttg aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt
    43321 agctgctcaa aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac
    43381 gcaacgatta gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact
    43441 gtcacaaaaa agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga
    43501 gtggcaaaaa aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca
    43561 aaaactgcaa aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca
    43621 agacgcagaa caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta
    43681 tcaaaatctc gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca
    43741 ggaaaaactg caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca
    43801 gcaagaaata gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga
    43861 actcaaaaaa gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga
    43921 acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt
    43981 tttgcgtcca ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt
    44041 aaaacacgaa cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc
    44101 tgagtctgct gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa
    44161 tcttcaacaa caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc
    44221 tgaacaagaa gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca
    44281 aaaaacaaaa acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat
    44341 ggccaatgca agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca
    44401 attagctgtc tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg
    44461 agaaatcaaa agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga
    44521 gatacgacga cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa
    44581 agcaggagtg caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca
    44641 tgaacaaata gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa
    44701 gttagaaagc cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca
    44761 agcacaatat aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac
    44821 agctgaccgg ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga
    44881 agagatcagc caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat
    44941 atacggttta caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat
    45001 ttatcacaca agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac
    45061 attcaacgga gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct
    45121 tcaagaagtg ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca
    45181 atttgagcta gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat
    45241 ctatgatgat caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt
    45301 tattgcagca ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg
    45361 tgtagcaatc gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt
    45421 ggaaatggca atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat
    45481 tagtcatgta agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg
    45541 tagcggacaa agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa
    45601 atggagtatt ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt
    45661 gttatcggtt gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga
    45721 attgtatatg gttgatttgg atggtacagc aaaagagata gattactgcc aatgtcccta
    45781 ttgggaggaa cataaatatt gtaagcatac agttgctgtg gaactttatt tacggtcgaa
    45841 aaatctatct cgtatcatga aaaaagacca gccaatgcta gaaccggtaa aagcaacaag
    45901 tgagggagaa atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc
    45961 ggtgcaacct ttgattattg aataccaagt agaaacgatt gagactaatc agtatcaccc
    46021 agagctttct attttggcac tttacttgcg tatcggttca ttggcatcac cgaaaaaaac
    46081 atatatcgta aaaaacatct atgatttttt acaagcttac acagaaaaag aaacttacac
    46141 tgttaataaa cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa
    46201 aatactcacg catcttgcag gaagtgctca gacgcatcaa ttattaggta cgaacggctt
    46261 acaagtcaaa ggaaaactag ataaaagata tctgcttctc ccagtagaac aagcccgctt
    46321 cctgttagag cagatgaatc agactacacg tttgcatctg cagataaatg aacaaaacga
    46381 tcaaggaata caattttccg atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg
    46441 agaagattat ttgctgcaag cattgaatga ttttgatttt ttctttatgc attatcagtg
    46501 gggagttatc caagggacat tttattctct gacgaaatat caacaaacgg tttatcaaac
    46561 ttggaaacaa ttgattcgcc gattggagaa accagaagta gtgttcaaga aaaaagaatt
    46621 accgtctttg tttaaagaga ttatccctct gctgtcagag atcggcgacg tagctattgc
    46681 ccctgcagta gaagaggaag tagcagatat ccccgtcgaa tttattttct ttttccgaaa
    46741 agcaaaagga aaaatccaag ctcgagtcga tttcgtctat ggtgaagtca tctattcgac
    46801 tgacccgaag catgaggtct cgacagaaca ggcttttcag gtgttacgtg acacagtaga
    46861 agagaaacga gtacttgatc tattcagaat gtatcgctac cagaaaaatg aaactggtta
    46921 tgaacgtcca ttgccaagcg gggaagaact atatgccttt ttccgcacag aactatctgt
    46981 cttccgtcag tttggtgaag tcagacttgg aagaaaattg cgagagttgt atttagatgc
    47041 gcataaacat caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg atattcgatt
    47101 cgatgtgaca gggatagagg agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa
    47161 tgatgctttt tatacattag aaaatggtca ggttctttct tttgattcag aagaatttca
    47221 acaaacaagc caagtgttgc aacaattacg ggaatcgata cgtacagaag aaggaacgat
    47281 ccatgtacct aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac
    47341 ttttagtgaa tcgttccaaa cgatggtaca agatctcatt catccagaaa gataccaggc
    47401 acagcttccc aaaggattga atgcaacgat gagagattat caaaagcaag gttttcgatg
    47461 gttgaagatg ttaggtcatt atcagtttgg cggtatttta gccgatgaaa tgggacttgg
    47521 gaaaacacta cagacaattg cctttttact ttcggaaaaa gaagaacgga aaagcttctc
    47581 tgctttgatc gtagcgcctg cgagcttaat ttacaactgg caggcagagg tgagaaaatt
    47641 cgccccttct ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg aagaattact
    47701 ggctaaagac acagatatcc gagtgacttc ttatgccagc ttaagacagg atctagctaa
    47761 ttatcagtcg caaaaaattg attatttgat tcttgatgag gcacaaatgg tcaaaaatag
    47821 cagtacgaaa actgcacaag cattgagaga attagctgta cctcagcggt ttgcactgag
    47881 tgggacacca atcgagaata atttagaaga attgtggtca ttgtttgcaa cgattatgcc
    47941 aggatttttc ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat
    48001 gattcgaccg tttattttac ggcgagataa acaaactgtg ttgaaagact tgccggaaaa
    48061 aacagagatg aatctatatt ctgcacttac agaagaacaa aaaactgttt atttagctta
    48121 cttgcgtcaa atgcgtgaag aaatcacatc aatggattct gaagcattca agaaaaaccg
    48181 gatcgggata cttgctggtc tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt
    48241 tattgaagat taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc tactggtagc
    48301 tgccaaagag aacaaacgaa gagttttgct gttttctcaa tttacaggga tgctgactat
    48361 cttgcaagaa gagttagccg agctagggat ctcgacattt tatttaagag gcagtacgaa
    48421 accacaagat cggttaagta tggtagacgc atttaatgct ggcgaaaaag atgtttttct
    48481 gatttcctta aaggctgggg gaactggatt gaatctcacg ggagcagata cggtcatctt
    48541 gtatgacctt tggtggaatc ctgccattga agaacaagct gctggacgtg cacatcggat
    48601 cggacaaaaa aatgtggtag aagtgtggcg tatgatctct gaaggaacga ttgaggaacg
    48661 catggattca ctccagcaag aaaaacgcga actgttccaa aaagtgatcc aaggtaatga
    48721 agaacagttg acgaaactga ctgaagaaga catccgcatg attttaagtg taggagaaat
    48781 cgaggagtaa gagactcgaa aaagcgttca gtaatgggta agaaagcctt tctaaagaag
    48841 cgattgttta tttgatttaa aaaatagcaa ggaggctggg attccatttt atcccagctt
    48901 ccttgctatc atttttttat ttctattttt aaaggttata caaagattgt cttcattgtc
    48961 tctaacagct gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg ttcaattttg
    49021 cgttctcgat ggttgaacca aagcgcttgc cagttagcac tttttgcacc tagaacatca
    49081 ttgtcgtaat tatcgccaac gtataaggtc tcttctggaa gcatatgaaa ttctttctca
    49141 gccagctgaa aaatctcttt ttctggtttt tggaaacctg ttgcttgaga aatgagcata
    49201 ttgcgggaag gaatccaatt gttcaattga agttgcttca gtttttttgt ctgatggtca
    49261 gtcggcccat ttgtgataat tcctaatggg acattctttt ccttcaagaa atcgagggtt
    49321 tttttgacct cctcatgcaa gcagatatta tcaagttctt cctcgtaaat tttttgaaat
    49381 aagaggccat cttcttccgt aatatgaggg tagtctaaat cttttagtga ttggctgatc
    49441 cgatgtgctc gcatatattc tagtgtccaa tctcctgcca ttacttttgg gaagttttca
    49501 tcactatgat ggcggaaccg gatatatagt ggatgcatat ctgcatctga aacaagcgga
    49561 acgactcttt tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt atcatcaaca
    49621 tcaaacacaa ctgatttaat caaaatttta cacgcctttc ggttttcatt gattttttca
    49681 caaatccttg actattgtaa cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt
    49741 tcttactata tttctatttc cttgacagaa taaggttttt atatttcagt ttttttcact
    49801 aaaaatcgtg aaacacgtca ataagtttga tgaacaaatt aatcattatg gtagaagtcg
    49861 attgatttat tttaatgcaa tctctgtgga attttcgaaa gatttatttt tttaatacag
    49921 tctca
//
LOCUS       DAZKOK010000006        47153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000006 DAZKOK010000000
VERSION     DAZKOK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.006"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..2343
                     /locus_tag="ACG90W_000334"
     CDS             199..2343
                     /locus_tag="ACG90W_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0476730.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2395..3435)
                     /locus_tag="ACG90W_000335"
     CDS             complement(2395..3435)
                     /locus_tag="ACG90W_000335"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0476731.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(3712..5514)
                     /locus_tag="ACG90W_000336"
     CDS             complement(3712..5514)
                     /locus_tag="ACG90W_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476732.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(5528..6280)
                     /locus_tag="ACG90W_000337"
     CDS             complement(5528..6280)
                     /locus_tag="ACG90W_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0476733.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(6289..6651)
                     /locus_tag="ACG90W_000338"
     CDS             complement(6289..6651)
                     /locus_tag="ACG90W_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0476734.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(6827..7699)
                     /locus_tag="ACG90W_000339"
     CDS             complement(6827..7699)
                     /locus_tag="ACG90W_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0476735.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(7750..8238)
                     /locus_tag="ACG90W_000340"
     CDS             complement(7750..8238)
                     /locus_tag="ACG90W_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0476736.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(8376..9167)
                     /locus_tag="ACG90W_000341"
     CDS             complement(8376..9167)
                     /locus_tag="ACG90W_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0476737.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(9222..9830)
                     /locus_tag="ACG90W_000342"
     CDS             complement(9222..9830)
                     /locus_tag="ACG90W_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0476738.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(9823..10140)
                     /locus_tag="ACG90W_000343"
     CDS             complement(9823..10140)
                     /locus_tag="ACG90W_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0476739.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(10296..11024)
                     /locus_tag="ACG90W_000344"
     CDS             complement(10296..11024)
                     /locus_tag="ACG90W_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0476740.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            11183..12064
                     /locus_tag="ACG90W_000345"
     CDS             11183..12064
                     /locus_tag="ACG90W_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0476741.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(12301..13344)
                     /locus_tag="ACG90W_000346"
     CDS             complement(12301..13344)
                     /locus_tag="ACG90W_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0476742.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(13347..14015)
                     /locus_tag="ACG90W_000347"
     CDS             complement(13347..14015)
                     /locus_tag="ACG90W_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0476743.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            14157..16493
                     /locus_tag="ACG90W_000348"
     CDS             14157..16493
                     /locus_tag="ACG90W_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0476744.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(16608..16919)
                     /locus_tag="ACG90W_000349"
     CDS             complement(16608..16919)
                     /locus_tag="ACG90W_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476745.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            17100..18575
                     /locus_tag="ACG90W_000350"
     CDS             17100..18575
                     /locus_tag="ACG90W_000350"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0476746.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            18622..19206
                     /locus_tag="ACG90W_000351"
     CDS             18622..19206
                     /locus_tag="ACG90W_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0476747.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            19208..19627
                     /locus_tag="ACG90W_000352"
     CDS             19208..19627
                     /locus_tag="ACG90W_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0476748.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(19908..20192)
                     /locus_tag="ACG90W_000353"
     CDS             complement(19908..20192)
                     /locus_tag="ACG90W_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0476749.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(20544..21254)
                     /locus_tag="ACG90W_000354"
     CDS             complement(20544..21254)
                     /locus_tag="ACG90W_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0476750.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(21251..22723)
                     /locus_tag="ACG90W_000355"
     CDS             complement(21251..22723)
                     /locus_tag="ACG90W_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0476751.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            22861..23334
                     /locus_tag="ACG90W_000356"
     CDS             22861..23334
                     /locus_tag="ACG90W_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0476752.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(23487..24038)
                     /locus_tag="ACG90W_000357"
     CDS             complement(23487..24038)
                     /locus_tag="ACG90W_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0476753.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            24143..24673
                     /locus_tag="ACG90W_000358"
     CDS             24143..24673
                     /locus_tag="ACG90W_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0476754.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(25156..25599)
                     /locus_tag="ACG90W_000359"
     CDS             complement(25156..25599)
                     /locus_tag="ACG90W_000359"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0476755.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(25615..26775)
                     /locus_tag="ACG90W_000360"
     CDS             complement(25615..26775)
                     /locus_tag="ACG90W_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0476756.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            27282..28982
                     /locus_tag="ACG90W_000361"
     CDS             27282..28982
                     /locus_tag="ACG90W_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476757.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(29084..29917)
                     /locus_tag="ACG90W_000362"
     CDS             complement(29084..29917)
                     /locus_tag="ACG90W_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0476758.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(30221..32386)
                     /locus_tag="ACG90W_000363"
     CDS             complement(30221..32386)
                     /locus_tag="ACG90W_000363"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0476759.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            32646..34940
                     /locus_tag="ACG90W_000364"
     CDS             32646..34940
                     /locus_tag="ACG90W_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0476760.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            34933..35598
                     /gene="pgmB"
                     /locus_tag="ACG90W_000365"
     CDS             34933..35598
                     /gene="pgmB"
                     /locus_tag="ACG90W_000365"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0476761.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            35591..36598
                     /locus_tag="ACG90W_000366"
     CDS             35591..36598
                     /locus_tag="ACG90W_000366"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0476762.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            36620..37639
                     /locus_tag="ACG90W_000367"
     CDS             36620..37639
                     /locus_tag="ACG90W_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0476763.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(37920..39287)
                     /gene="nhaC"
                     /locus_tag="ACG90W_000368"
     CDS             complement(37920..39287)
                     /gene="nhaC"
                     /locus_tag="ACG90W_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0476764.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(39396..40817)
                     /gene="tyrP"
                     /locus_tag="ACG90W_000369"
     CDS             complement(39396..40817)
                     /gene="tyrP"
                     /locus_tag="ACG90W_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0476765.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(41060..42937)
                     /gene="tdc"
                     /locus_tag="ACG90W_000370"
     CDS             complement(41060..42937)
                     /gene="tdc"
                     /locus_tag="ACG90W_000370"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0476766.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(43228..44484)
                     /gene="tyrS"
                     /locus_tag="ACG90W_000371"
     CDS             complement(43228..44484)
                     /gene="tyrS"
                     /locus_tag="ACG90W_000371"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0476767.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(44596..44801)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            45064..46365
                     /locus_tag="ACG90W_000372"
     CDS             45064..46365
                     /locus_tag="ACG90W_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0476768.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(46434..47033)
                     /locus_tag="ACG90W_000373"
     CDS             complement(46434..47033)
                     /locus_tag="ACG90W_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0476769.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        14466 a         9727 c         8375 g        14585 t
ORIGIN      
        1 aggtggtcta tgcaataaaa tgaacgtcaa agaaaaacga atgaatgctc gttgcttatt
       61 tacaaattgt tttatttaga atacacagac agatttatga aaagaaaaag cttttcgaca
      121 cgattatctg ttccaaaaaa tgtctaatga atgctgaata agacagaaaa tcaatgaaag
      181 gaaaaggact agtttattat gaaaaataaa aaaacgatac gtttgctagc tgctacttgg
      241 ctgattgctc cgtctgtttt gtcgaccaat accgtatttg ccgtagataa tcaaatagct
      301 gatcaaacag tcacaacaga tacgactaca tccagctcag ctactagtga aacaacggat
      361 tcttttacag aagacccaac tggaacaact aactcaacaa caggatcgac cgagccaact
      421 gaaattgatc cggctgatcc atcagaaaat gaaaagccag ccgagacata ttcccttaca
      481 attgctcctt ctttgattgg taagatccaa ctgactgctt cgacagcgga ttcccctgaa
      541 cagcaacctt tggatattac ggaaagtgga acggtagaag gtttattagc ggaaacgaag
      601 gtaacttaca ctattacgcc agctgaaggt gaacaaattg cttcagtcgc tgttaatggt
      661 gccaaagaag taaattatga tgcaaagacc tttacagtag gtacgaacaa tttatctatt
      721 actgcagaat ttaaagctgg accggatact tcaacagaga aaccaactga tcctagtgaa
      781 gaagtcccta gtacaccaga tccggaaacg ccaaacaaac cagatgtggg taataacaac
      841 aataataatg gttcaaataa ttcaaacaat aataacaata attcgaacag cgggaattct
      901 tcgaatcaaa caaacccatc tggatcgaat aacaatggaa atactcagag agaaccaaac
      961 catccaaaca atggttcatc gaatggcgga acgaacaatg cttcatctcg tccaaataca
     1021 gcaaatcaaa gcggtataga gaatccatct tctgcttctt ctgattttgt tgtaaaatca
     1081 ccaattgatg ctgtattgcc tacaaataca acgaatgtcc aacaagcaat cgttcgagaa
     1141 gcattcaagc atcttggaaa accatatgta tggggagcaa aaggtccaaa tacttttgac
     1201 tgttctggtt tgacttatta cgtatatatg aaagcaaccg gtcattatat cggtggctgg
     1261 acaggcgaac agcaatatgc aggtacgcag attccagtaa gtcaggccca acctggcgac
     1321 ctcgtcttct ggggaccttc ttccggtgtt actcaccatg taggtatcta tattgggaac
     1381 ggacaattta tccatgcgcc tcaaccagga gataaagttc gagtaacttc tatttcagat
     1441 ttcacgcctg attttgctgt ccgagtaaac ttagcaggtt tacctgctgc ttcaggttcg
     1501 ctgacaggcg gctcatctat tttagatgga ttagatggct cgttccattt cagtcaaaac
     1561 caaacaactg atcagttctt gaaaaagatt gcagatgatg cacaggaaat cgggcaaaaa
     1621 gaaggaatct acgcatcagt catgatggct caagctatcc ttgaaagtgg atctggaaac
     1681 agcttattat caagcgaacc aaatcataac ctttttggta tcaaaggatc atataaagga
     1741 agcagcgtga ccttcaatac acttgaacaa gatagttccg gacaaagcta ccaaatccgc
     1801 gcacaattcc gtaaatatcc ttcttacaag gaaagtttgg aagactatgc cgacttgatc
     1861 aaaaatggac tgactggcaa tcctgatttc tacaaaccaa cttggaagtc ggaaacaaaa
     1921 gactacaaag aagcaaccaa atatttggaa ggacgttatg ctacagatcg ccaatattca
     1981 caaaaattga acgcgatcat cgaagcgtac gacctgacaa aatatgatga accgaaaaaa
     2041 gaagcagcaa caactgaaga agcaaacgct gaaatcctca atatgtctaa gcgattcgat
     2101 gtgccaatca aatggacaac agatggcgtg ccaatcacag gattagaatt ttcaacgata
     2161 aaacgcgtca acaacgcttc ttccatcttc cgtgtcgctt caatctggga tttgtggaac
     2221 aattttacgg caagacagat accagaaaca actactcgta cagttaaagc tgcaactgtt
     2281 cctgtactaa ccttgttaag tttagacaaa tcattctggc ttgatctgcc tttaaagaaa
     2341 tagaataaat taaaaagggg atgtgacact gtcacactcc ctttttaatt tattctattt
     2401 ctttatattt attttgtatt ccgttacaaa ctcgcgtact tggttaggtt ccagagtgac
     2461 atctccaaaa ctttcaagtc gctccgctcc tggcagttct tgagtctcaa atgtgatacc
     2521 gccatgattt actaatttat tcccgcgcat ttccggtcca tcttctccaa aatttgctgt
     2581 aaaaatcacg attcctgaag catctgtttt tacttggatc gatacttttt catctgggct
     2641 tgtcaattcc gcagcattta gtaaaccaga ttgttttttc aacaaaaatg gatgatcgat
     2701 gccgcccact atcattgatt ggcgaaaatc actggcaaag acctcaccta attttttagg
     2761 acgttggaag tcaaatgctg tgccacttac tgaaactact tctcctgtag gcgttgtatc
     2821 tttattgagc gtagcaaata atcggctatt caaccaaagt atatgttcgt cgattgactg
     2881 tgctggatca ccggttaaat tgaaatagac atgatttgta ggattgaata ctgttttgcg
     2941 gtcactgatc ccttggaccg tcactcgcca tgtatcatcg ttcgtcagcg tataccgtac
     3001 ttctacatct aaagttccag gaaatccgtt ttccatatct gggctagtct gatagaaaat
     3061 gaccgatgct tcgttttcac cattgatgac tgtataatgc cactgcttgg cttcaaagct
     3121 aggtgcacca ccatgtaaac aatgaccagt gtccgggtct gtttttactt cgaacgtttc
     3181 atcatctaat ttgaatgtgc cattcgtaat ccgtccagcc atacgtccga ttgaagcacc
     3241 gatataaggg tctttttcta gatattcttt tgcttcatca aaacctaaga tcaactctcg
     3301 tttttcatta tcgattattg tttgaaaatg aacaacccgt gccccaagat cactgacaga
     3361 caaagatgtc ccattttgat tcgtgatcgt aatcaagtga tagttttcgc caaaattttg
     3421 ttcattgatt tccatgttcc ttgcaccctc ttttctttca tttgcactga ttccagtata
     3481 catggaaacc gttttcttaa cagcttattc cgtgagtaac gagaaaaaat ttactatatt
     3541 ctaataaaag accgtgaaaa aaaggttgtg acaaaaatat tgtcacaacc tctttttccg
     3601 aacaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg
     3661 aggagctatt tttgcttggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3721 ctttttcaat acgattccag tatataacca tgtcgtcagt acatagtaga tgaaatacaa
     3781 tacgaagaat atcgtaaacg gaacccaaat atctttgtat ggattttgca tgaatagacc
     3841 aaacatttgt aatccgaata agacatggac tacacctaag attcctggtg ctaagaataa
     3901 cacgcctact tctctacgga tcgattgttt aagcaacccg cgtcgtgttc ctatcttttc
     3961 aagcatttca taacgagcaa tgtcactctt gcttcctgac aagatcttga acatcaaaca
     4021 gctagcaagc atggtcaaga aagctaaacc taggaagaat cccataaatt caaaaccaga
     4081 gaatagacca ttatacactt catacatttc atatttctgg ttgctgtact ctactttttg
     4141 caaagatgga ttgttcattt tattttgagc aacaagtgtt tcgattggtt tcaggtttgc
     4201 ttggaagtct tttacctgaa ctagctgtaa agtggattgt ggcaagttca gttgatcaaa
     4261 ttctgattga ctgactacct tgatttcttt ttcttgctgt tccggcaatt catagcttcg
     4321 aagttcctct tgtacagtca gttcattcat ttttttagca ggaacttttt ctttcgtgat
     4381 cttcggcggt gttccatctt catgttttat catgatcaat ggttgagccg caaattcact
     4441 tgcattatag taaatcgttc ccgcatcttc tttttgagta taagaagcag aaagtgttgg
     4501 tgaaagttct ttgatttttt gctgatcttc tgcccgagga ttattcatca ctagatcata
     4561 tccagaattg ttttctgtca agtctgcaat ttcattttta aaacctaatc ctaccgtcaa
     4621 agcgcccagt gccaaggcaa acagcatagc gaccattgag agcatttgtg tatattcacg
     4681 aatacgaaag tttaactgtg acaatgtgaa attgttcaat tttttcaatg aaatctgttc
     4741 tgatttcttt agtaatgata aaaagaaaat gatcacagaa tggaaaatga aataagttcc
     4801 taatacgatc gtaactaagg ctacccctat accaaagact tgataggtcc ccacgtgttt
     4861 catcatatag taaccgcagc ctagaaaagc aattcccagt actacttcaa ggaaaaacaa
     4921 gaatttatgc tgcttgattc ttgttggtgt ctgatccgca cgaatcaatg ctaaaatcgg
     4981 ttttttgaca atcgaaaaag cattgacaac agcagctaat aaaaacaaaa cagcaaaaaa
     5041 caaaacagtg atcaacaacc cgtgaatact aaaaggtgta aaatgcgaaa tattgagatc
     5101 taactgatcc actaataatc ggtggacgat acttgtcaat cctacaccga cagctgaacc
     5161 cactaaagta gctgcgatcc ctactaagaa tgtttcagaa aaaatcatct gtgcgatttt
     5221 tctgcctttt gctcccagca tcataaacat tgcatagtct ttttgccgca tcgtcatcaa
     5281 aaaggaattt gcatataaaa tataaacgaa tgtgatgatc cctagtaaaa cagaaccgaa
     5341 tcggaaaatg ataactgtag ccgcaacgat cgtattactt ttcaaaaaag cttcgttgct
     5401 tgccattgat tcaaacatgt aaaaaatagc agatgccata actaatcctg agaataacac
     5461 caagtagtct cttaaacggc tcttgatccc agtaagtgat aatttccaaa gcatagtcaa
     5521 tctcccccta ctgttccaag tttccaagaa tagatagtat ttctcgatag aaggaatcac
     5581 ggctttgatc tccacgtttc acttcttgat ggataccgcc atccttgatg aataaaatac
     5641 gttggcaata acttgcagaa aacggatcat gcgtcacaag aagaatcgat acatgatctt
     5701 ttgtattcaa ttcgtccatc gtatctaaaa gatcccgggc acttttggaa tccagcgcac
     5761 ctgtaggctc atcccctaat agaatcgttg gttgtgtgat caatgcacgt gcggcggcga
     5821 cccgttgttt ttgtccaccg gaaatctcag aaggatagct ttccaaaata tgagagatgg
     5881 ataaacgttc tgcaattttt tggacacgtt gcttgatttc ttttggtctg acgccttgta
     5941 aagaaagagg aacagcgata ttttcttgtg ctgtcaagct ttctaataaa ttgaaatctt
     6001 ggaaaataaa gccaatctca ttcgcacgaa aatctgctag ttgatttcct tttaatgtgg
     6061 tcacatcttt ttgattgatt cggatatgcc catctgttgg tttatctaat gtggataaaa
     6121 tattcaaaag tgtcgactta ccagaaccag aagcgcccat gatcccaata aattcgcctt
     6181 tttctacatc gaagctgatg ccgcttaatg cttgcgtctt tttctcattg ttttttccgt
     6241 atacttttgt tacattctgt acttcgacta ttttgttcat ttctttccct accctttaat
     6301 tagtttttcc agtgctgttt tcggcacgtc ttttttttcg acttcttcat acgaagtgac
     6361 acctttattt ttgttccagg tgatttttaa atatgctcct ttacgaagcg gtcgagtcaa
     6421 ttgtcccttg aagtccagtt ctttttcctt accctctttc gtatagccag gcaggatata
     6481 tctatagttg actgtttctt gtccagagtc tgattttgaa actgtttttg taccatctgt
     6541 cgtaatttgt acatagtatt cttctccacc cataactacg taatctgcga ttttcacccc
     6601 tgcaagtacc acaacgatga aaacaagtag accgatcaga agttttttca ctttttcccc
     6661 ttctctctta ctttatatac tgattataga aaaaagcgaa caattctgcc ttcgaatcaa
     6721 ctatcattct tctttcaatt ttgtcatttt ctttaaaaca actaaaaaaa gagttcaaga
     6781 caataatccg tcctaaactc cttcttccta tcttacaaaa agtatgtcac ttacggtctt
     6841 tcatgtcgcc tgtattgata acaggttgcg ttcctttgtc tgtaatgatt gagacaagta
     6901 aattattgat caattggact ttccgatcat cggtgaaatt gatttcttgt ccatcttcga
     6961 tttgctctaa ggccatttga gtgattgtga cagctccttc aacaatggtt tgtcgagcag
     7021 acaaaatggc ttttgcttgt tgacgttgga gcatggcact agcgatttct gttgcatagg
     7081 ctaaatggtt caagcgtgtt tcgattactt cgacacctgc tactgctaaa cgctcttgca
     7141 gctctttttg caactcgtca gacactgccg tcgtgtttcc tcgaagcgtc aaatcattct
     7201 cattgaatgt atcatacggg tattggctag cgatatgccg aatggctgtc tcactttgga
     7261 tttctacaaa atcttgataa tacgctacat cgaataatgc ctttgctgta tcaacgactt
     7321 taaagactac tactgctgag atttcgatgg ggtttccatc caaatcattg acttttaaaa
     7381 cagaactatt gaaattccga actttcaacg aaacagtcat tttttgtgcc aaaggtatcg
     7441 taaggaaaaa gccattttct cgaatcgtac ctaaatattg tccaaaaaac aaaattactt
     7501 ttgcttgatt aggactgact acagttgccg aacttaacaa caataacgtg atcaaccaaa
     7561 gaaaaatact taaaaaaagt gcccaaactt tcgcttgcca catacctaaa tagaagaaaa
     7621 acaatccaac taaagctaat acgaccaatc cgacaatccc tgcataacca ttgatataaa
     7681 atgctctttt ttctctcatg ctacttcctg cgctccttta tccttcaata aatatctaat
     7741 gttacttttt taattttttt ttcgaatacg cgccattaca ccttctagtt ctaactcttt
     7801 gtcttcagtg atgtaccaac cgcttgcctg tgtccctccg gctgcctgcg gtaaataatc
     7861 caattttgtt cgaacccagt gcttttcttc tgtaaaatag tcttctacca agacctctag
     7921 atccatccct gctgaaagag gcaaatcttc ccgaacagat ttaaagataa atcgttcctt
     7981 cttcccatca tacttcaata ttcctgtatc gataatcggt aaagaataat aatccagctc
     8041 tttcttcacc gtcaataaat gtttgaaaac ttcatggtac acttgctcta attggcgacc
     8101 ttctgaattg ttcgggtaat taaccatcac tttttctttg tctggatcat agaaggtttg
     8161 gcttagttga gtgatataag tatgtaatct ttccatttct gtttttaact tttctctttg
     8221 accttcttct accttcatat actctttcac cctgttttct ttttagatat tttctttctt
     8281 attttactat agttatgtgc gttaagcgat gaaaaacagc aaagattgga aggatctttg
     8341 ctgttttttc ttaaaatatc cagttgagta caggatcaat ctgtttcatt gtcctcttga
     8401 tccgtcgtaa tattgatcga tgtgtcttta gcagtgatct ttagcttttt ctgtccttgt
     8461 tctaacaaag tatttgttat tggctggtag tctttttcaa tataagattc accagacgtt
     8521 tggatagtat agccactgtt ttccatctca tgcaaagata aattgccacc gtttaggatg
     8581 acttttcctt ctttgctgat caaactgtct tctagatgga agttgctgtt ttcacctgtg
     8641 acgatcaagt tatccacatt ggcctcatct aatcgaagtg agcctaattc atctttcgca
     8701 gttattgttc ctcctgtaaa ttcattgaac gatacccagc catcatgtgt cttagtattc
     8761 acttttttga ttgatacttc gttgatatca attgcactat tattcccttc gattgtcaat
     8821 tcagataatt catgatctgc agggacaaca atagtgattt gtgcacttgt gtcttctaaa
     8881 ctaatgattg cacctgttga ttttttgact gcattcacag tcaacacgcc atttttctcc
     8941 tttatttctg gcttttctgt cttatcataa gtcgttttta tctcaaaatc caatccgcgt
     9001 atgaccgaaa ctgtttgatt ttctgcatgg atgacgattt tgtttgcatc cttgattttt
     9061 tttgtctcag taaccatatc gatcaaccga ggaccattat cccaagtcag actcttttga
     9121 gcgcctaatg tataagttgc acctgctagg aggccaccag taagcatcag accaataccg
     9181 gtaatcaaaa aatgtttcaa tctcaattta tgtgtcctcc ttcaaacgcc cgctgaatgc
     9241 agatctcttt ttgtaataaa acgtcgtccc atccatttaa caaagttcat catgacataa
     9301 aaaagccctt ttgttccttt cagcaacagt ggcgaaataa atactgcgct tcctgtggcg
     9361 aacagaccga caccgataaa gaataatcca ctcagaatcg attgtccaaa cacactgatt
     9421 cctgagatga tcatgaatag gccaacacct aagatactga tacataaaac atagatacta
     9481 aaaatgagcg ctagtaaagc aaccaatagt cccccaataa ccaatataaa ccccagtgct
     9541 agtggaatga ggacaggaga agcaaataaa ccgataatga ttaaccaaat caggcgaatt
     9601 tgtctttttg gatttttcac taactcgttt tctgcttgtt cttcttctga aaagtaactg
     9661 atttttaatt ttaaggcaaa ctgctttggt gtaccatatt catcaatgat ctgatctaat
     9721 gtttttccag aatctaaaaa atattcctca taatactgga tcaaatcaaa acgatcgtct
     9781 tcaggaattt cttgaagatg atccacaact tcattcaaat attcattcat ttgtgctctc
     9841 ctccaataac cggtctaaag aatctcgaaa aacaatccat tcatcaataa tttcttctaa
     9901 atgacgactt cccattggtg tcaacgagta atatcgacgg tttctgcctt caaaaggttc
     9961 atcataggtc tcaagatatc cttctttttt caatcttctc aaaaccggat atagtgtaga
    10021 ttcactgaca gagagacttt cttgtacgat ttttgttaag gcatacccat acatatcttc
    10081 cttgcttaat gctgctagca cagtaccatc aagaagaaca gttggaatct ggatgtgcat
    10141 tacgtcacct tctattctca agtgtaatat tttatttcaa tctatattat acaacatata
    10201 atatagtttc aaaatcagtt tgtcaaatac aaaaaaactg actaaaagat ttgatccttt
    10261 ttagtcagca aaaaatggct tctctttgat ttttattata acagtggcat aatccaattt
    10321 gtccacatcc agctcagtgg catgataacg atcattgcag gtatcatgtc agctactggg
    10381 aacattttca cgttgatcat tctaaaaccg gtagcgacca tcaagaatcc gccgcaagct
    10441 ttaaaatcta agatcatgtc tggtgtagtc aatggataaa taaattttgc caataagaac
    10501 aaaatataaa aaataatgaa ttggggaatt gctatcacag aaacgacata ccctaaatta
    10561 cacgcaaaaa tagctgctgt gaaaaaatca aggacagatt tagaaatcaa aatcgtactg
    10621 tctcctgtca tcccagaatc taaacttcca tagatccccg taccacttgc acaaaataga
    10681 acaatgaccg tcactaatgt gctgataaat tcatcgtggg tcataccaag cttttcacta
    10741 gggaagattt tagaaatagg tacttgcatc agcgcagccc ccttatttat cccattacct
    10801 aaatgaacaa tcaaaccaat cgctgttcct atcactaaag caaaaataac agccggcata
    10861 tttttcatcg gcgcaatgga atagattccc atccccatcg aacacacacc aaaaaccatc
    10921 gtcagctgtg ctttaaaatg ttcacttaat ttatttccta ctaatccgcc aattagtccg
    10981 ccaaacaaaa cggaacctac attgatcaaa actccagttg gcatcaaaaa actcctctct
    11041 ttttctacga agcgaaaaaa atcagaaaaa tttctgtaat tattgctttc gctgcaagaa
    11101 agattgtaac agacagagaa gattacacca attcattttt tgtgttaaag tattctttat
    11161 tggaataagt aggaggaata tgatggatat ccataaattc gaagtttttc ttgatttagc
    11221 cgaaacaatg aatttcacca aaactgctga tcgacaattc accacccaag gaaatatttc
    11281 taaacagatt ctttcgttag aaacagaatt agacgtcaaa ttattcgaac gcgcccatag
    11341 aaaaatcgaa ttgaccgaag ctggaagctt attgctccca tatgtaaaaa atgtcgtaga
    11401 acaataccat gctatgcaag aagtgcttga aacctatacg aaagacaaca atctttcatt
    11461 aaatatctta acgataccga caatgatcaa ttataaaggt ttttccaaaa tcaccgagtt
    11521 tttgaaacgc catcctgaat ttacagtcca gttgaaagaa gtggaaagtg tcgaattaac
    11581 ttacaatgac gccaatgaac atagtgacac gatccacttt gctagaagtt tccaggcccc
    11641 accaagttcg attgagtggt tgcctactga aacagacgat tttgtagcag ttctacctaa
    11701 aaatcatcct ttagctagta aaaagaaatt ggatctaagc gaactgaaaa aggagaactt
    11761 tttgctcctt ggccccacta cgaacttgta tcaacctgta ctagatctgt gtcgcgaagc
    11821 tggatttgaa ccaaaaatct cttataaagg agcaagaatc gacttgatca tcaatatgat
    11881 tgcaaatgac atggggattg ctattgttat ggaaaaaaca gtcaagaatc tattaaaaga
    11941 aaatactgtg attctcccta tttctccaac aacagagagt tatcttgcat ttactcgtaa
    12001 agtcggtgag cattctttgg cttcagatac attttgggag tatttaaatc aagaagaaaa
    12061 ctaagcccta cattttctgt ccatctatcc acccaaaaaa agagagcgac gcatgacttt
    12121 cgctctcttt cttgcatcat gtccttgttt caatgacacc tctaacattt ctcgtagttc
    12181 aataagaaag cagcgatttt tatgccaaaa gaaccgtaag aacatttccc taatcacaaa
    12241 aaaacgacta aaaaccccta aaaaccttca atagaatttt taggggtttt attctttttc
    12301 ctattgttta cttaaccgta tcattgctgt cgttcctact ccttcgatac tgctataatc
    12361 aatacgtcca tcatgcagtt ctagaatttt tttaaccagt gaaagtccta gcccattccc
    12421 agaaatttga cgacttgtat ctccttgata aaatttttca aacattcgat ctctggtctc
    12481 ttcattcatt cctacacctg aatcggtcac ttccagtaca atctctgtgg ctgtctcaaa
    12541 tagtttgata tgaatctgtc cattgatttg attgtacttt atggcgttat ccatgatatt
    12601 cagccacact tggtacaaaa actcttcatt gcctgtgtaa tttactgcag caagttcgat
    12661 atccaattct agctgttctt tctcccattt aggctgaaaa aaaacgatta cttctctgat
    12721 ttgttcatct aatcgatact ctttcttttc cagttggata cgctggtttt ccagtttgtt
    12781 caattttaag atgttctctg ttaattgtga aagctgcgtg atactttcga taatccgata
    12841 taaaaatatt tggcgttctt cgtcagagag atttggggct tgtaacaatt gaacatatcc
    12901 ttggattgtt gctagtggtg ttttgaattc atgggataca ctggacacaa agtcattcct
    12961 cagtgtctcg atactattga gttcttggac cataacgttg aaatctttat acagttgctg
    13021 tacttctgct actttctgct gttcatccat tcgaatcgaa aaatctcccg ttgctaccag
    13081 actcatattt gtccctagcg cactgattgg atgcaggata cgccgcccaa caaaaatcga
    13141 tattcctgta cccacgacca tactgaaccc cgcaaagacg aataacgggt ataacgttgg
    13201 ttttccagcc gattcagcag gcgagataga atgtcgagac aataagaaaa taaaaccgag
    13261 aaaacacagc agtgtcacta agatcgaaat gaagacaata ctagcaaaat agatccataa
    13321 ttgtgaaaat agcttttttt tcatctttaa ataaccgcct tatagcctag tccccgtact
    13381 gtgatgattt gaaaatcttc cgtcttttca aaacgggtcc ttaaacgttt gatatgtaca
    13441 tcgatcgttc gttcttctgt attcgtatct aatccccaaa tatcatccat caactgttgg
    13501 cgagtgaaga tcttgttggg ataagacagt aatttataca gaagcagaaa ttctttttga
    13561 ggcagaatca acgaatgctg atcttgtaac actgtataag catctagctc taattttgtt
    13621 tgtccgatta tcaagctact gctgtaattg atctttgaac gtcgaagcaa tgcttccaca
    13681 cgtaggatca tctcattgac atcgatgggt tttgtcatat aatcatctgc tcctaatttg
    13741 aacccgcgtt tcttatcctc aaaatcggcc tttgccgtaa tcattagaac aggtgtgtca
    13801 aatctggtgc tacgtaatga gtctagcaag tcatagccat ccattttcgg catcatgata
    13861 tctgtaatga ttaattctat acgtgtcgct tctagttttt tcagcgcatc ctctccattc
    13921 aatgcggtga tgactgaaaa gcccactcgt tccaacacag aacggtaaag caactgtaat
    13981 tgcggatcat cttctactac taaaatacga ttcattgatc tccctctgtt ttttgaaaaa
    14041 tttctagatt ttttcttgta gttcatcttc agttaacatt tatttcgtat actacgatca
    14101 ttgttctgat atacttatta gtataccgaa tatttcaata cgggagaagg gtgattatgc
    14161 ttcaattaaa agatataaaa aaatattata aagtcggcga aaccaccacc aaagcattgg
    14221 atggtgtgtc tgtcgctttc agacaaaaag aattcgtagc gattttaggt cctagtggtt
    14281 ctggtaaaac gactatgctg aatgtgattg gtggactaga taattatgat tccggtgata
    14341 tggtaatcaa cggaaagtct actaaagact ttaaggacag tgactgggat gcctaccgaa
    14401 acaactctat cggtttcatt tttcaaagtt acaacttgat tggtcatcta ggcattattg
    14461 aaaacgttga acttgggatg acattaagtg gtgtttctaa agacgaaaaa cgaaaacggg
    14521 cagaagatgc attgcaccga gtcggcttga caaatcatat gcataaaaag ccaaatcagt
    14581 tatctggtgg acaaatgcag cgagtggcca tcgctcgagc actagccaat gacccagata
    14641 tccttttgtg cgatgaacca accggagcgt tagatacaga aacaagtatc cagatcatgg
    14701 aattgatcca agagttatcg aaagaaaaat tagtgatcat ggtcacacat aatcctgaac
    14761 tggcccatca atacgcagat cgaatcattg aattttctga tggtaagatc ttaacagatt
    14821 cccatcctca catcgaacga ccaaaagatg atcaatttaa cttacgcaga acaaagatga
    14881 gtttttggac agcgttaaag ctttccttca acaatatccg aacaaaaaaa ggacgtactt
    14941 tcttgacctc ctttgcttcc agtattggga tcatcggaat cgcgatcgtt ctgtcccttt
    15001 cttctggatt ccaaaaacaa atcgataaca cgcaagctga aacgatggcg aaatttccga
    15061 ttactatttc taaggtaacg actaaccaga ctcgtgacga tgccggattg ggtgcaagca
    15121 aagcggatta tccagacagc aaaacgatta ctgcaaaagt cagcgaagaa gataaagcac
    15181 agcacacgaa taagattgat caaacatacg tcgattacgt aacagacatc gatcctaact
    15241 taagtaataa tatcggattt acccgtacta caggaatcaa tcttttacgt gatgtaaatg
    15301 gcaaagtaca gcctgtcagc ttctcgaatc aaaatcctga tacggaatct ctttctttat
    15361 ctagtacgat gtcagcaatg acaggtgtgg gagtgtcaag tttccctaca cagcttgaca
    15421 ctagcaaaga aaacttttta aaagacaatt attctttact tgccggaagt tatccagcct
    15481 cagcaacaga tgtcgtgctg atcgtagacg gcaataataa tactaatatc aacgcattga
    15541 agaatcttgg atttgatgtg aaagaagacg aaaaacttga ttttgatgat atcgttggta
    15601 caacattcaa attagtaaac aacaatactt actatacaaa acttccaact gggaatttta
    15661 tccctaatac agattatgat gcgatgtatc agaatgcttc tgatgaattg aagatctcgg
    15721 ggatcctacg cgtgaaatct tcttctacaa tgaatctctt atctccagga atcgcttata
    15781 gcgatcaatt aacgactcaa atcgtcaacg aaaacaaaga atcagaaatc gtcaaagctc
    15841 aaagagacag tggtgtaaat gtcttaacta ctgaaaaagt tgacgaaaat gccaaacaaa
    15901 cattgcttag ttatttaggt ggagacagct tgccttcaag tatcatgatt tatccgaaca
    15961 attttgaaga caaagaaaag atcttggatt atctagatga ttacaataaa ggcaaatcag
    16021 atgaagataa aatcatttat actgatttag ctggaacgat gactgaactg actggcggat
    16081 tgatggatgc tatcacttat gtccttattg cttttgcagg gatctcttta gtaacaagta
    16141 tgatcatgat cagtatcatt acttatacat ctgtgattga acggacaaaa gaaatcggtg
    16201 tactaaaagc tttaggtgca cgtaaaaaag acatcactcg cgtatttgac gcagaaacgt
    16261 gtattttagg tatttcttct gggatattag gtgtgtttat tgcatggcta gctacgttcc
    16321 ctatcaatag catcttatac aacatgacgg atctaaaaaa tgttgctcaa ctaaatcctg
    16381 tccatgcgat tattctagtg atcgtctcta ctatattgac gatgcttggc ggtcatcttc
    16441 ctgcacgaat ggcagcgaaa aaagacgccg ctatcgcttt gcgtgcagaa taaggttgtg
    16501 acaaccacat ttagatttga gtcataagag cgctgaaata gtagaaaaca gcaagaaacg
    16561 aggaagtaat atgttccttg tttcgcgctg ttttttttaa ttatttttta taattctggt
    16621 atacttttct ttcctttgta ccacaactct tctggcgtat acctgtcttt tccatttatg
    16681 aatactgttc catctgcttc aatcgttact tcagtcccaa tccacatatt acgagaaagt
    16741 gcgagaagat tttttctttt tcctcttcct ttttttccaa attgcaaggc atcggttatc
    16801 tcttctgtcc aatgagattt cacgtatttt acttctccgt tttcaaatgt aactttcaaa
    16861 atattcgcta tattttccac ttttactgct ttaggaaatg actcctccat ttttttcact
    16921 ttccttcctc caacactaag tatgcgcttt cttttagtga taagtatatc atagtttatt
    16981 ctattatcaa aaaaacgcat ccattccatt gtagaatact ttaccgaaca agttgaattg
    17041 ttttcctctt tttcaccttt tactatagat aggtaaagac aactaaggag gaatctctca
    17101 tgacagatca accgtatgat ttaagaaaag tcatcgaaga actaaaaaca cttccaggtc
    17161 aatatcacga aacagatatt gaaatcgatc ctgaagcaga attatctggt gtttaccgtt
    17221 atatcggtgc tggcggtaca gtaaaacgtc cgactcaaga aggaccagct atgactttca
    17281 acaatatcaa aggtttcccc ggcacacgtg tgaacatcgg aacaatggct agtcgtaaac
    17341 gtgtaggtga aattttgcac catgattatc gaacacttgg tcgtttcttg aaagacgctg
    17401 tagaaaatcc aatcaaacct gtgaaagtag acaaagcaga cgctccagca caagaagtcg
    17461 ttcatctagc taccgatgca gacttcgata ttcgcaacat tttgcctgca cctacaaata
    17521 ctcctgaaga tgctggtcca tacatcacaa tgggtgtagt cttaggatca gatccagaaa
    17581 aaacaatgag cgatgtaacg atccatcgta tggttctaga agataaagat acgatcggta
    17641 tgtacatcat gcctggcgga cgccacattg gtcacttcca aaaacaattc gaagccttga
    17701 ataaaccaat gcctatcact atcaatattg gtttagatcc agcaattgcc attggcacga
    17761 ctttcgaacc acctaccact ccacttggct acaatgaact atggtgtgca ggagctttgc
    17821 gtaacgaacc tattcaaatc gttgatggta tagcagtcga tgaagtgggt attgctcgtg
    17881 ctgaattcat cattgaagca gaaatcatgc caaatgaaac aatgcaagaa gatatcaaca
    17941 cgaactcagg aaaagcaatg ccagaatttc ctggctatac tggggatgct aatccagctg
    18001 tacaagtagt gaaagtcaaa gcagttactc accgaaaaga caatccaatc atgcaaacaa
    18061 cgattggtcc cagtgaagaa cacgtatcta tggccggtat tcctacagaa gcaagtattc
    18121 ttgccttggt cgataaagct attccaggaa aagtagtaaa tgtctacaat cctcctgcag
    18181 gtggtggtaa attgatgacc atcatgcaaa tccgtaaaga aaacgaagcg gatgaaggta
    18241 ttcagcgtca agcagctatt cttgctttat cagccttcaa agaattgaag acagtcattt
    18301 tagtcgatga agatgttgat attttcgaca tgaacgatgt agtttggact ttcaacactc
    18361 gtttccaagg agataaagat atcatcgtga tcccaggaat gaggaaccat ccattggacc
    18421 catcagaatt accagaatat aatcctggct ctattcgttt ccgtgggata agtgcaaaaa
    18481 ctatctttga cggtacagtt cctttcgata tgaaagaaca ttttgtacgt gcaaaattcc
    18541 aagaagtaga agattgggaa aaatatttga aataatgaat gatctgcaac tgttttgaca
    18601 tcataaaaag gagtgcggaa aatggaacaa ttggcacaac ggaagaaaaa aatcgtgatt
    18661 ggtgtaagtg gcgcttcagg caccatttat gccatcaacc tagtgaagaa actaaaagaa
    18721 tatccgatga tagaaacaca cggaatcatc agtccatggg caaaacagaa cttaaagctg
    18781 gaatccgact tatcactaac tgaattcgaa agcctgtttg attaccatta ttcaaacaaa
    18841 gatttaggtg ctgctgtcgc cagcggatca tttttgaccg atggaatgat catcgtaccg
    18901 gcaagtatga aaacagtcgc aggtatctct gttggtttag gcgacaacct gattgcacgt
    18961 gctgctgatg taacaatgaa ggagcaacga aagttagtca ttgttcctcg tgaaacgcca
    19021 ttaaatgcca ttcacttgga aaacttgact aaattagcac gactaggtgt gcaaatcata
    19081 ccaccaattc ctgcttttta taatcagcct caaacgatcc aagatcttgt taatcaccat
    19141 acaatgaaac ttttggatca atttgaaatt gtaaatgagc attcaaaacg ttggcagggt
    19201 gattaatatg gaaaaagaaa cgatagttga agtaaccaaa ctaggtttca cgatccaagc
    19261 tgctgttaaa gtaatcggta aagacttatt gatcacttta acaggcggag atacaccaca
    19321 tatcggaaca gtcactacat tttctcgtga tacagagatg caaactgtac gtttccctag
    19381 ccacgatggt cggttccata aagacgatgt cttagccgaa aaaatagctg tgatcatcaa
    19441 aactgccgtt ccgggaagtt gtacgatcac ttccggtatc catgttgacc atatctccaa
    19501 tgcacaaatc aacgcctctt ttccgatgtc agaagagtta ggttacaaga ttttagattg
    19561 gttggaaaaa atagatttcc atatcaaaga accagtttat tataaagatg gtgaaaaacc
    19621 attatgaaag aagaggttgt gaaatcagcg ttatgacctg agtatgagag aaaaaaatgg
    19681 aaaaatagct tctcatattt tgccgttttt gagctaatac cgagggacag ctgattgaac
    19741 gccgtttatc aagaggttgt gataaagccg cttcgtttcg agtagtaaga aaaattttgt
    19801 aaacatgggt ttccacattt ttgcataaat ttgacttact accgagaaac tggcttttga
    19861 acaccgttta tcaagaggtt gtgacacgtc ttttgtcaca acctctttta tttttgcttc
    19921 actgtaactg ctacaatatc accgatttct tttccgatgt ttttacgaat atcttttcgt
    19981 agtccaagga tgtagcagac tgaaccatct ggatttttca cgcccatatt cacgatactg
    20041 ccttcatacg gcacattgtc aaaagttgct tggactttaa ctcttccagc accgaattct
    20101 tttcttatat catacggaaa aataatatat gccccgccct tgccgacttc ggaagcatag
    20161 atttttgatt caaaagaata aatttccctc attttatttc tcctattttt tacatctagt
    20221 atagcatagc aaaaaaaaga aggaactgtg acaaaagtct tgtcgcagtt ccttttttcg
    20281 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatagtca acaaacatga
    20341 gaagctgttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
    20401 ttccttaaac ggtgttcaaa agcttgcatc tacggcacta agcccaatca gatgaaaaat
    20461 atggagtgct atttttatct gattgttctt actgcttgat gcagagcagc tttttcacaa
    20521 cctcctacat ggtgttcaaa atattattcg ccttttgctt ctttcactaa ttttgcgaat
    20581 gcttgttgtt ggaattcagc agctaaggct ttcgtacgtt gttcttgctc tgctttgctc
    20641 atttttggat tttcagtcat tgcttttacc ataccgtctt gaacataagc ttttgcttca
    20701 ctttccatcg tactttgctg tttcttcact aattcagcta acttggctgc ttggtccgta
    20761 cttaattcta cccatgtctc tgggattttg aatgtattca ctcggctgtc atattcgtga
    20821 ttgttatcag caatattgaa ccagaattta tacatgtcgt ttttgtattc ccaataagta
    20881 gttttcgtca ctttttcagc atcgccgtct gtcttttcta ctttatttgt ttcgttatcc
    20941 gtaccagttg cttttggttt ttcttgtttt tcatttgttc ggtaaagata cactttttct
    21001 tttccattcc ctaaagcttt atacaaaagc atatctgctc cttgggaatc agcactggaa
    21061 acaatttttt ctgtttttgt ttccgttact ttttccattc caaagtgatt ggacaaatta
    21121 gcgacgatca aacttagtga agcgacgaag attagtccga aaatcaaaga taaaaaggtt
    21181 tgccatgttt ttttagcgaa cacgttcgtt aaagcaaaag caagcaagct gacaatcaaa
    21241 actccaataa tcatgcatta tcccccgctt tcattagaac aggttctttt ttcttcaata
    21301 aaaaggcaat aataaagcca agaatcccaa agatgatcgc tacgataaac gcggcatggt
    21361 aacctgacag tgtggcattg attgcctgat ctttataagc taatggcgta ttatccaata
    21421 cagatttgct tgggatattt gcttttgtca cattcgtcaa cacactgatc aaaatagctg
    21481 taccaatcga gcttgcgatt tgacggaatg tattatttac cgctgtccca tgactgatca
    21541 agttatttgg caatgcattc ataccagaag tcgttaccgg catcatgacc atggagatcc
    21601 caaacatacg gatcgcataa aggatcacga tatataaaac tggagtcgtt tttgtcaaga
    21661 aagcaaatgg tgcagttgcc gctgtcaaaa tgaacatccc tgtgatggct aaacgtttcg
    21721 cgccgtattt atcgaaaatc gcacccgtca caggcatcat agcccccatg atcaatgcac
    21781 ccggcaacaa catcaagcca gaatggaatg ctgattctcc acgaatattt tgaatataca
    21841 atggtacgac catttctgcc cctaccatcg ccatatttgt tacaccagct aaaacagctg
    21901 caatcgtgaa tgtagatgat ttgaatacac gaagttccaa gaatggatgt tccaaatgta
    21961 attgacgcca tgagaacaag gcgatgaaaa tcacaccaac aactaagaaa ccaattactt
    22021 tcaagcttcc ccagccgtca tctcctacac tagaaaatcc gtaaagtaaa ctaccgaatc
    22081 caatcgttga taaaatagcg gataaaatat caatgctagg atttgacaat ggaataacac
    22141 ttttcattag gaagaatgct aaaaccaaga caagaacaac gattggcagt accataccaa
    22201 acaagtcacg ccaatggtag ttatcaatga tataaccaga taatgtaggg cctaaagcag
    22261 gagcaagtcc aataacgatc cctgacatcc ccattgctga tcctcttttt tctggcggga
    22321 aaatagatag catgatattt tgcaataatg gcatcgatac tcccacaccg caagcttgga
    22381 tcaaacgacc gattagtaat gtactaaaat taggtgctgt aaagcaagta atcgttccga
    22441 tcaaaaagat cgtcatggca gaaagatata aatttcttga actgaattta ttgatcagcc
    22501 atgctgttat tgggatcatg atcccgttga ctagcaagaa accagtcgtt aaccattgca
    22561 catcagacgc tgaaatatcg aaagctttca tcaatgtagg aaaagctgtc gtcaataagg
    22621 tctgattcaa aacggtacaa aatgtaccaa ttaataagac aacaactaaa agtgtccgat
    22681 tatatggttt tccgtaaata tctacgggtt ggttgttact catatttctc cttctttctg
    22741 aattttttct gaaactacta gaatacttta gtcctaaatt cgctatttgt caacctactt
    22801 gacacataag ttaatacgga tataattaag ggaagcaaga ggacaaaagg agtgacaaaa
    22861 atggttgggg cttcccttaa acgtttgttg gaaaatgatc aattggttgt tggcatcagt
    22921 gaattaagtg atatcgtagg tgtgtctcca cgtcaattaa ggtattggga acaaaaggga
    22981 ttcattcgtt cgatcgaagc tgatgcgaat tgtcctagaa aatatcgttt acctacagtc
    23041 ataaaagtag aactgatcaa aaaatattta gatgacggat ttactttgtc aaaagctgca
    23101 gaaaaggcag aaatccgcca aaagaaaatg caccatgtcc gtaaagtttt ttcaaaagca
    23161 atcaaagata tcgaattgat cgacgaacgc tatacgattt tacaaatcgg taacttcaaa
    23221 gaagagaatg ctaagatgta catcattcat gacaacgaga cagacgaatt aacttacaag
    23281 ctccttcctg tcgatgaaaa agctgactac gaacagttgt taaaagaact ctaaacgcca
    23341 ctaatctcct aagtaccaag ctttttaata agtagatgct acataaatac ttgaaaaaag
    23401 gcctgtttga acataagaat atccgaacgg tgatctgcgt ttctttcata tagaaaggaa
    23461 ttctccatca ctgttcggat atttttttat ttttttcggt tactttcttc taatacatct
    23521 gttagccaat ttgcatattc tgtttcccga ttgatcgcat gcgctaaaat caggtagtgt
    23581 ccgtaatttt catgtttttc tttttcagta ggaaagatcg ttttttttcg ctctaataga
    23641 tgaagtcgtt tttcttcatg aaggtcacgc tgctgctgaa taatttctac taatgccgga
    23701 tcattcacgt ctttgacgaa atacaatttc agaacaaatt cgtctcgatt aaccggtagt
    23761 tcattggtgg gtgtatgaat ccactctgcc aattgttcct tcccttcttc tgttaattca
    23821 taaactttct tttccaattt ttcacctgtg atctctactt gatgctgaat gattccttgc
    23881 tcttccattt ttgcaagttc aggataaatc tgactatgtt ttgcttgcca aaattctcct
    23941 atttcatttt tgaacgattg attcaattca tatcctgtca tcttcttctt acttaataac
    24001 cctaacaaaa tatatcgaag tgtatttttt cttgccatca gttttccctc cttgacaaat
    24061 ctttaaatcc attctatcat aaatgagtaa tcgaaaacat gtagtttatt acatgtttta
    24121 atttataagg agatgcttaa acatgaaaga atttaaagaa ttggaagatt ttattggaac
    24181 tcactttatc tatacgtatg acaatggttg ggaatatgaa tggtatgcca aaaatgacca
    24241 tactgttgat taccgtatcc atggcggaat ggtcaaaggc cgttgggtaa aagaccaaga
    24301 agctgatatc gttaaattga cggacggcgt attcaaaatc acttggacag agccaacagg
    24361 gacagacgtg gcattggact ttatgccaaa tgaaaagaaa cttcacggaa tgatcttttt
    24421 ccctaaatgg gttgaagaac atccagaaat cactgttact ttccaaaacg aacatatcgc
    24481 agaaatggaa gcagctcgtg aaaaatatga aacttatcca aaattacttg ttcctgaatt
    24541 tgctacgatc acttatatgg gtgatgcagg tatagacaat gaagatgtta ttagcgaaga
    24601 accatacgat ggaatgccag atgatatccg tagcggaaaa tacttcgatg aaaattataa
    24661 acgagtaaaa taaggttgtg aaagagaggt tgtgaaatca gcgttctgac ctgagtatta
    24721 gagaaagaaa cggaaaaata gttcctcatg ttttgccgtt ttttgagcta ataccgaggg
    24781 tcagctgatt gaacaccgtt tatcaagagg ttgtgaaatc agcgttatgc tttgagtaat
    24841 aagaacgcta gttagaaaat agcttttcat attttctagg ctagtgggct tattaccgag
    24901 aagcagccgt ttgaacaccg tttatcaaga ggttgtgaaa tcagcgtttt gacctgagta
    24961 ttagagaaac aaatggaaaa atagttcctc atattttgcc gttttttgag ctaataccga
    25021 gggtcagctg attgaacacc gtttatcaaa gattgtaaca actgcatatt cacacagtta
    25081 aattagtgac ttcaaaatga aataatgctc ccagcaaaaa attaggctgg gagcattatt
    25141 tcgttattct gttcatcaaa aatcagcgtt ttgattccat ttcatcacaa gttcctcctc
    25201 atttactgct tcatcttttt gctgtgtttg aattgtaaaa ccttgtgaaa gatagaaatg
    25261 aatagctgat tgattttttt gatagactgt caaagtcagt gttttattgg tttcttttat
    25321 ttttttcatt agttgcgtac cgattctttg ttgccggtat tcccttttca caaagattcc
    25381 tgcgataaac tgatcaatca ccccagcaaa accaataatt tcttgatcta tttctgctaa
    25441 gtaaatcact gcatctggta aagctttcct aacagattct acttgattct cccaataagt
    25501 tgatggaatg aattcatgag aatccaagtt agcatgcagc caaatcgata gaacagattc
    25561 cagttctttg gcagacaatt ttgttgttgt gcggatcatt ttttctctcc ttacttattc
    25621 attgatttta aaataaaaac catttttttg aagctctttt acaagttttc gcccctcaaa
    25681 aaagtatatc ttctcttgtt ttacttttct aataccaaaa gaaacagcaa ctttaggatc
    25741 atcagctgaa acgattcgat aagttccacc aggcaattga tccagcttga ctttcccatg
    25801 atggtcgctt tgtactgaaa acggcacacc atttctagta agtgtttttt tcttatgttt
    25861 caaaatgaat gaactaccat ttaccggttt ccctgacgaa tcactaataa taaatgaaaa
    25921 atcaagcact tttttactta atctatactg atagattagt cgtccgataa acactaacag
    25981 tatgactaca gctattatcg ttcctgcaag aataaaccat tgccgcatgg ttctttcctg
    26041 atttcctttt ccttcttctt tttttaccga ttcgttataa ggaatccgat gtcctgtcac
    26101 tagcaaacga tgcgtgttga tcatatacgg cgtacatgta agcaaggtaa ctaagtcttc
    26161 tccttctgaa atcgttagaa cagacgtgtc atctggcagt accacttgta tatttttaac
    26221 ttgataagcc aatttctccc ccaaaacagt caaaataaac gtgtctccgt tttctaaacg
    26281 atctaattcg gtaaataacc gtcgattagg taaaccgctg tgagcagaga tcactgaatg
    26341 cgtcgaattt ccaccaagcg ggaaagaggt tccttgcaag acgcctgctc cattttcaag
    26401 gatcctacca ttgagccgat cataaagtga aatatttaat tggatcttag gtatattgat
    26461 acttccgatc agccattcct ctaattggct ggatgttgta ctttcatttc tgttcttttc
    26521 attgaaagga tcagtaccag gattgaatcc ttggtcagtt aatcgttggt ttttctcctc
    26581 cattttcttt cgctgcactt caatttcagc tgcggtccgt ttttgtactt gttcgatacg
    26641 tttttgatca ataaaactat ttaaagaatc cacataaaat ggatataaag cgatcagtaa
    26701 ccctacgacg atacatatta ataaaaacaa acgaaacaaa agtcgacgag actgtttttg
    26761 cttactctgc ttcacatgtg tcctcccttg caagtttttt tgttctttct tttattatat
    26821 gggttcagga aaaaaataga aaatgaaagc cgatgaggtt gtgaaaaagc tgtcctgcat
    26881 caagtaataa gtacagccca aaaaaatagc tactcatatt ttttgttggg ttgggcttat
    26941 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaaa ctgcgttatg
    27001 ctttgagtaa taagagcgct agtcagaaaa tagcttctca tattttctag gctagtgagc
    27061 ttattaccga gaagcagctg attgaacacc gtttaccgag aggtcatgaa aaagctactt
    27121 tgtttcgagt attaagaaaa attctgtttc aaaacaagga gatgcaacaa aacttatgtc
    27181 gcatcctctc tccttagaac tttcacttat gtttttcttg taaaattcat aaatattttc
    27241 atgaattttc taatttcttg cctacctctc gcataggata tatgatgaat ttatctaaag
    27301 tttgcaaagg agttcgacaa ttgaaaaaat atggcccatt tttagcaatt tttttcattg
    27361 ctttggcatt tctcatgcct catttattca cacataaact aattttgggt gctgatgccc
    27421 cattccatta caatcggttc tatgatgccg cgcaacaaat taaatatggg gatttccatt
    27481 attttccttc cctttatgga ttccagcaaa gtggcaggat cgtgaatgcc gtttacggcc
    27541 catttttcgc ttatttacaa ggattgatcg tgttgcttgc aggtagctgg tttcattatc
    27601 agattctttc aaatttcatt ctttatctta tttcaggatt ttctatgctg attttactga
    27661 gatatgtcaa tgttcgaaat tgggtaagtg taccagtagc cgttttattc atgagtacgt
    27721 attctattca atactggtct atcaaccaag gattcactag ctgggggact tgtttttatc
    27781 ctttatgtat gatccctatt gctgatttcg tccttcataa aaagttccct gttatcaaag
    27841 tcgctgtctc agtgggactt atgacacaga tccatttact gtcttcgatc atgttagctt
    27901 tgatgtatat ccctttttat ctttattact tttttaacca gactgaaaaa ggaaaagcac
    27961 tgcttcaact gattaagtcg gtcttgcttt attttgttct gacagcaaat atttgggcag
    28021 gattagctat tgtttacagt gggaataaga ttcttcctcc ctttgttaac agagaaatga
    28081 gtcaaaaggc gatagatctg gatggcagtt attggctcca ttaccctaag gcctttccta
    28141 tattgatatt atcaggtttt ttgcttttta ttttctttca acaaaagaac accttattcc
    28201 aaaaaatcgt gttgtacaca agtcttgttt tcttttttct ttctactagc ttgattccat
    28261 ggacgacatt gatcgaaaaa aatgtgcctt ttattagttt gatccagttt ccatttcgat
    28321 ttttcgtgcc atttacgcta ttgcttctac tttaccttgc cctttcactg aaccagtggt
    28381 ctgaacaaaa gtggttccgt cttttaagta tatttggcat tcttattttt tcagcacaga
    28441 cgatccagaa cttataccag catctggaaa agtgggaaaa tgaaacattt gtcagtcgac
    28501 atacttatct attcgatacg ccggaagaag caaggaaaac attcttcagc aaagatatgt
    28561 ctaccagctt gttcgttttc caaaaaacca cacctgacta tctgccaatc tataaggaga
    28621 caaaagacaa taaatacgac cggtataacg aagaaatact agaaaacgaa acaactttta
    28681 taaaaacaca tccaggcggt cttttgacaa tcaagtggaa aaacactggg gacaaaactg
    28741 ttcatatccc tgttatcgtc tacgatcgga ctgtgctaca gcaaaatgga aaaacattga
    28801 ctgattatga agtaacagat atcgggaccc caatcgtgaa gcagcaaaaa ggaatcaacg
    28861 agctcacgct tcattatcaa acgccaatct atttctattt cattctttct cttactttga
    28921 ttggctggtt taccttgcta tgtttattta tttatcatag atacaagctt ttacgtgctt
    28981 aggcaaaagg gataaataca tccgatcaaa aaactctcca aacggtataa tcgtttggag
    29041 agttttattt taatcagttt ttttatgtta tatgttatat tttttattcg ttcacatcta
    29101 attcgatttc caccccgtag tgatcactga tgattggact atcgatccca tcaaaaatcc
    29161 gttgatattt tttaacctgc attccttcag ggacgaaaat aaaatctata cgcaattttt
    29221 ctgtattttg ttcccaacca tctattttct tttccacagt agctgctttt cctttttcat
    29281 tcgcgacaac atacgaatct tgaagaagaa ggcaactttc tcttacagta taataacctt
    29341 gagagtctac tggattattg aaatcaccta ggaaaaaaag tggtacgcga gtttccaaaa
    29401 agtagttttc caagtttttc cactcaaaat aaaagccact ttttttatct gtccaccaag
    29461 aaaagtggca gctcgagaca aaaatgtcct gatcatctat ctttgttttc ccaatcaaga
    29521 tcttgcgtgt ctgataatct tctttctttt gaaattggga gacggtaagg acctgacttt
    29581 ccaatgtttt tttcgtaagc agtgcatttc cttcttcata tttatcataa ccgatgtggc
    29641 tcatttccca actccaaaaa tactcttttc cgtgttcctt taaaagctga actaaacggt
    29701 aggcaaaatt atctttacgg ataggtactt gattctttac cggacagaac ttcattagct
    29761 tcgcaggttc tacttcttca gattccagta attggttgat ttcttgtaaa gcaatcactt
    29821 cataatcttc taacaggatc ttatctgcta attcttgcaa ttttttctct ggttctggct
    29881 caagccagct atgcgtgttg agtgtcatga atttcattgg ttttctcctt gtcttcattt
    29941 cgaaacaaaa gggttgtgac aaaatttttg tcacaacctc tccagcaaat agtgttctcc
    30001 tgctaactcc tcagtaataa gctcaatgga ccaggagcta ttttttgttt ggctgttttt
    30061 atcacttggt gcagggcagc tttttcacga cctctccact aaacagtatt caaactgctg
    30121 cttctcggta ataagcccaa ccaaacgaaa aataggagga gctatttttg tttggctgtt
    30181 cttatgactt gatgcaggac agctttttca caacctctcc ttacatttta atggagccga
    30241 tcactttacc agctgtcact gtacctaatt gcttgatcac tacttcttct actttatcgt
    30301 ttgtaaatac gatcatgatc gttggatctt tttcttcgtt ttggatcaca tcaagatcca
    30361 tctcagctaa tttcgttcct gttcctactt cttggccttc tttgacagca atctcaaaag
    30421 ctggctgatt catttggact gtatcgattc ccatatggat caaaacttcg ataccatctt
    30481 tcgtttccag cccaattgca tgtttgctag ggaaaatact tgtcactttt ccagcgatcg
    30541 gcgcaacaac ttctcttgat gttggaataa cagcaaagcc gtcacccatc attttttgtg
    30601 agaataccgg atcttttact tcagataaag gaatcacttc tccagaagct gcggaaacta
    30661 gttcttgttc tatacctaag tattgcttac tacctgcttg tccatttcct gtagaaacca
    30721 tttctggctc tggaatctct gctcctgatt gcagcagatc ttcgatatct gatttcaaga
    30781 catctgcttt tggtccataa actgcttgga ctccattgtc tttgacgatc aatcccatcg
    30841 cccctgctcg tttccaggct tcttcagtcc ctactttttc tcgatcattt acacttacgc
    30901 gtaaccgagt catacaagca tcgacatctt tgatattttc ttttcctccc agcaaatgaa
    30961 tgatctggac cacttgctga tctgctgttc ctgttccgct gttagataca acggtttctt
    31021 cactgtcatt gtcgtaattt ccgttacgtc ccggtgtagc aaagttgaat tttttgatca
    31081 agaaatttgc taagaaatac gtaacaacac caaaagcgat cacacacaac acgaagttga
    31141 taagatctcc acctagaccc gctttgatag ctaacggtgt ccgagtcagc aattcgatat
    31201 tcccaaatga atggacccgc aatggcaaga tatctgccat cgcaaatgca gccccttgga
    31261 tcactgaata aatgacatat aatggcacag cagcaaacat aaacatgaac tccaatggtt
    31321 ctgttacacc tgttagaaag acagccaacg ctgctgaaaa atacattgat ttatatcttg
    31381 ctttcttatc aggatcgaca ttacgataca tcgcaaaagc cattcccatc aagatacctg
    31441 aagaaccaat catttgtcct actttgaaac gagccggcgt ccagttatgc aacacaaact
    31501 catattgcga catatcccct gcacctttaa gattcactaa atcagtggcc caagccaacc
    31561 ataacggatc ttgcccaaat acttgtgtcc ccgcctgtgc gcctgataaa atctcatacg
    31621 atccgccaag ctgtgtatag ttgataggaa tcgtcagcat atgatgcaat ccaaatggca
    31681 atagtaaacg ttctaatgtt ccaaataaaa acggtgctag aatcggtgcg ctttcttgtg
    31741 attctgcaat ccataaacca aaattattga ttccagcctg tacgtttggc cacaccacag
    31801 caagaatcaa tgccacgatt gttgaccata agataacaac aaacggaaca aagcgctttc
    31861 cattgaaaaa tgacaaagca tccggcaatt ttctataatt atagtactta ttataagcca
    31921 ttgccccgac aaatccagcg atgatcccta cgaatactcc catattcaaa gctggtgctt
    31981 ccaatacact tgtaaaaaat cctttaacca tgatcttcgt accgaacaga gtgtgggtaa
    32041 atgcatcttc attcgcaagc atgtcacttg tgacaccaaa gatagcccca gtgatccgat
    32101 tgattaaaat aaatgagatc ccagctgcaa atgctccgcc tgctctttct tttgcccaac
    32161 ttcctcctat tgctaaagca aataaaatat gaagattacc aataattgcc caaccaatat
    32221 tttcaattac tccgcccgtt gtgacaagcg gcgtccaatc aggattgatc aagggaattg
    32281 atttccctat actgatcatc aacccagctg ccggcataac tgctaccaca accattaaag
    32341 cctttccaaa tttttgccaa aactcaaaac taaatatctt tttcattccg tttagctcct
    32401 ttacgcaatc ggtttcgtta aacgtattat agcgtgcgtt ttcttttttg taaatgcttt
    32461 attttttatt tttctgtcta taccattcta taaaacctat aatacgtgtg ttcccttcta
    32521 taattttctc ctaaaaattt atttttttaa gcttttttct atgaaatatt ttcttttgtc
    32581 tattgacttg tgcaatcggt tgcgtttatt atgaacgtat aaaacagctc attaggagga
    32641 gaaaaatgaa acagatcaaa cgactatttg ctattgatcc atggaaaatc agcacgacac
    32701 aattagataa aaaaaatctt cgactgcaag aatctttaac aagtattgga aacggttata
    32761 tggggatgcg cgggaacttt gaagaaactt actcagggga ccaccatcaa ggtacttatt
    32821 tggcgggtgt ctggtatccc gataagacac gtgtcggctg gtggaaaaat ggttatccag
    32881 aatactttgg aaaagtgatc aacgccttga actttatcgc gatagatatt tcgatcaacg
    32941 atcagctgat cgacttggct tctttggaac cagaagactt ttactgggaa ttagatatga
    33001 aaaatggtgt attatctcgt acattcacaa tcactacttc tactaataaa gtacgcattt
    33061 ccttcgaacg ttttctgagc attgtcaaaa aagaatcagc ttatatctat ttaactatag
    33121 aaatgcttga aggtagcgga acagtcaaac tcgtatcaaa attggatgga aatgttcaaa
    33181 acgaagacag caactatgat gaacattttt ggaatgagat tgatcgtgga actatagaag
    33241 aaatcggcta tttgacgact aaaactattc caaacaactt tgggattgaa caatttactg
    33301 ttactgcagc aatgcgccat ttcgttaatg gaaaatcagt catccctgac tatcacgaag
    33361 atactttagc attacatggt tgcttgtctg cagaattatc tcaaggtgag acatggtcag
    33421 tggaaaaaga aattatcgtt cttactagta gagatattcc agaagcgcag caagcagaaa
    33481 aagctgtctc agaattaacg aaccagacaa acaattatat ccaagcaaaa gaagagcaaa
    33541 cagcggcttg ggcagaacga tggaagcttg ccgatgtggt catcgaagga gacgaagaag
    33601 cacaacaagg tattcgattc aatttgttcc aactattctc tacttactac ggcgaagatg
    33661 aacgattgaa tatcggcccc aaaggattca caggagaaaa atacggcgga gccacttact
    33721 gggatacaga agcgtatgct gtccctcttt atcttgcttt agccaaacct gaagtaacta
    33781 agaatctgct gaaatatcgg cataatcaac tgccgcaagc aatccacaac gctcagcaac
    33841 aaggattaaa aggcgcgctt tatcctatgg tcaccttcac tggtgtcgaa tgccataacg
    33901 agtgggaaat cacattcgag gagattcacc gaaatggcgc gattgcctat gcaatctaca
    33961 attatacgaa ttacaccggc gatacggaat atttgaaaaa tgaaggactg gaagttttag
    34021 cagaaatcgc acgcttctgg gcagaccgtg tccatttttc aaaacgagca aataaataca
    34081 tgatccacgg tgtgaccggc ccaaacgaat atgagaacaa tatcaataac aattggtata
    34141 caaatacgat cgcagcttgg gtcttgcgtt acacgcgaga aaattattta acttatcaaa
    34201 aagaagctgc tatcactatc agcgaagaag aactagccaa atggaaagat atcgcagaga
    34261 acatgtactt cccagttgac gaagaattag gaatctttgt ccagcatgac acattcttag
    34321 ataaagatct catgcctgta tccgatattc ccacaagcga tcttcctctg aatcagcatt
    34381 ggtcttggga caaaatcctt agatcttgct tcatcaagca ggcagatgta ctacagggga
    34441 tttatttctt caatgatact tttacaaaag aagaaaagca aaaaaacttt gatttctatg
    34501 aaccaatgac tgtccatgag tcttcccttt cgccaagtat ccattcgatt ttagcagcag
    34561 aattaggaat ggaaagcaaa gctgtagaaa tgtaccagcg aacagctcgc ttggatttgg
    34621 ataattacaa taacgacaca gaagacggct tgcacatcac ttccatgact ggtagctggt
    34681 tgtcgattgt ccaaggattt gctcaaatga aaactgccca tgatcagttg agattcgcac
    34741 catttctgcc aaaaacttgg aaagcttatt ctttccatat caattatcgc ggacgtttat
    34801 tgttcatctc agtcacagat gaacaagtta ctttcgagtt attagaaggt tctcctctag
    34861 aaatttacgt ctatgaacag tcttattctt tagcggatcg tctagaaata acgctaagaa
    34921 aggaggaagc aaatgtttaa aggggtattg tttgatttag acggtgtcat taccgatact
    34981 gctgaattcc attatcgtgc ttggcgaaag cttggtcagg aaatcggcat tacgatcgac
    35041 cacacattca acgaacagct aaaaggcgtc agccgagaag actcactctc tttgctttta
    35101 gcttatggcg gaaaagaaca ctctttttca aaggaagaat ttgcagaatt agcgaaaaga
    35161 aaaaacgatt attatttaga gatgatccag acgatcgaac caaaagatgt cttccccggg
    35221 gtcgtgcctt tactggattc tttaaaagaa gaaaaaatca agattgctct tgcttctgca
    35281 agtaaaaacg ggccattcct acttgaaaaa atgggtctta caccttactt tgatgctatt
    35341 gccgacccgg cagaagcaac aaacggaaaa cctgcgccag atatcttcct ccttgcagca
    35401 aaagctgtcg gactaaaagc agaagaatgt atcgggatcg aagacgcgca agccggtatc
    35461 caagccatcc tctcaagtgg cgctcaacca attggcgtgg gacgtgctga agaccttgga
    35521 gaggagatcc cgcttgtccc tgatacgact tttcttacga tcgattactt gaaagaaaag
    35581 tggcatgacc atggataatc cagagatctc cgtcgtacca gttccagaga ctgaaataaa
    35641 gaaaatcaca ttgaagaacg actacttaga agtcgttctt ctcaattacg gtgcccgcct
    35701 gcaccaaatc ttcgcaccag acaaagaagg aaaatcagaa aatgttttat tgtcttacga
    35761 tcgtttcgaa gatgttctaa cagacaaaag cttttttgga gcaactgtcg gacctgtagc
    35821 cggaaggatc cgggatgctt cttggggtga gcatagattg gagaaaaact gcggatcaca
    35881 ccatatccac ggtggcacga aaggctggtc tttccaattt tgggaagtag aagttttcaa
    35941 aactccatca tcaataggtg tggtctttta cttaaaagat gaattttcta cctatccagg
    36001 accaatcact gctacgatca cttaccgttt aacaaaaaac gaattagaga tgacaacctc
    36061 tgctagtagc cttgttgaaa ccatctgcaa tccgaccaac catgcctact tcaacctttc
    36121 aggaaatgga acaagagaca tctatgaaca tcagttaacg gtctttttag atggtgtgct
    36181 tgaattagat caagaaaaac tgcctactgg aaattggaca aaaaaagaaa aactgccaat
    36241 cgactttaga aaatccccaa cactgcaaga gatccttgca tgttatccag atggactaga
    36301 tgatgtattt cttttgcatc atcctaggct ttccaaaact tccttacaat tattcgagaa
    36361 gcattctggt cgccaaatga ctatagcaac ttctaataaa agtatggtat tattttctac
    36421 gactggattc gaagctgatt tctctgtcaa tggaaaacag atgcattcaa actacggatt
    36481 agcaatcgaa ccgcaagaaa tccctgatat cgttcatttc cccaaatttg gatcgatcaa
    36541 cctccatcct ggtcaagaac ggatcagtca gacgatttat cggttctctg cacaataaca
    36601 agagaaaggt gtttttatca tggctattac cgtaaaagat gtggcgaaaa aagccggtgt
    36661 tgccacctcg accgtctctc gtgtcatcaa cgatcatcct agtatttccg agacaacaaa
    36721 gaagaaagtc cgaaaaatta tggatgaact aggttatgtt cctaacatga ctgcccgcaa
    36781 tctaggaaaa cgcatttcca gtgccatcgg cgtaatctta ccccctcttg attcaaaaga
    36841 gcggatcggt aacccatttt atcttgagat catggaagca atcaacgaag aagcgcggaa
    36901 ttatgatatg actacagcaa tcgctactgc taaaaatttt gaattgctgt tagagaacgt
    36961 ccagcgtatg catttacaaa aacaagtaga tggttttatt ctagtgtatt cagatagtca
    37021 agatactgtc attgattatt tataccaaaa ccacatccct ttcactttga tcggacagcc
    37081 ttatcatcat gagaacgaaa tcgtttatgt agataatgat aaccagcttc taggtaaaca
    37141 agcaacagaa tttttgatcc agaatggaca taagaatatc ctgtttgtga caaacacgac
    37201 tcatgaaagt ttgtatttcg aacgatattt cggttatcaa aaagcaatga tgatggctgg
    37261 tcttcctgcg ttatccagtg tagtactaga gcatccggag gattatctgt cctttgagaa
    37321 aacgttgaaa gaaaacgaag ccacagcatt ggtggtcatt gacgatatct tcgcccttcg
    37381 cacgatgcag ttggcgcaaa tgtatggcta tgtcattcca gagacactct ccgtcattag
    37441 cttcaacaac tcgatcttct caacattaac acacccttac cttacaagta tcgatatcga
    37501 tatctccgaa ttaggacgtg tcgctatgca aaagctgcgc gaattgatcc atgaagaatt
    37561 gagcagtggc gtacgtttgg tcatccctca tcgcttgatc aaaagagaga cagtcgttcc
    37621 tttagaaaac ccatactaaa tagctgactc actaaatacg ctgaccattt atcctatggt
    37681 cagcttttta tgtaaaagaa gatactactt ctaacacatt gtttatctgt aaaaaagagc
    37741 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
    37801 cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttaaaaag caattttctt
    37861 gagtttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttccttcctt
    37921 caatttttta cacgtcgacg attcaataaa aaaccgccta atattgtaaa gacaggtgca
    37981 agaatcgaga aaaaggcgaa tggcaagtaa tgcaacgcgc taactttcat cgttcccata
    38041 atgaatgtgc cgctgactcc ccacggtacg agagaattga cggtcgcacc tgaatccgct
    38101 agtgttcgtg tcaaataggt ttttgataag ccaacgtcat caaaagaacg cttgaatgtt
    38161 tctcctggaa gaatgatgga cagatactgt tctccaacga tcagattgat tccgatacag
    38221 ctcaaggcag taaacccgac taaacgagaa ggtcggtcca tcttttcttt taattcttga
    38281 acgatggtat caacgatcag atatttgatc agtaacccac ctaatgccaa tgcaagtaaa
    38341 atcaaactag cagaacctaa catactcatg atcccgcctc gagaaagcag ttcatctagc
    38401 tttggatcag gtgtatttgc gacataacca ttcatcaaga tgtctgctgc ttttccgacc
    38461 gtcaaacttt tctcatggat aaaagaaaga acgagagcag ctgtcgatcc cgctagtaat
    38521 gtagggatag ccggaatttt tttgattgcc cccccaaata aaataactag tggaatcaat
    38581 gtccaaggag agatccaaaa gctcgctttc atgttttgga tcatttcatt gatactagct
    38641 agatcggctg aatgactgtt atgtcctaat atcgtataaa aaatcaacga aaggacgaat
    38701 gctgggaaat ccgtataacg cataccagcg atatgttcga atagattaac tcctccaata
    38761 cccgctgcca agttggtcgt atctgataaa ggcgagcagt tattccctaa aaaggcaccg
    38821 gaaacgactg cgcctgtagt cattgcatca ttgaatccaa gtaaatgacc gatccccaaa
    38881 aaagcaattc caatcgtcga aatcgttgta aatgaactgc cgacagtcac tccgacaatt
    38941 gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag aaacaagttt aaatccataa
    39001 accataatcg tagggatggt tccagataaa atccatgaag caactaagac accaatcaaa
    39061 agaaaaatca ggattggaat gatccctgga gtgattcctt tgatgatccc gtcatggatc
    39121 tcatcccatg tgaacttttt aaatctgcca taaaacaaca aacacatgaa agctgcaagg
    39181 atcgggatat gtggcgagag ctggaatccg atgatcagta ctcctaggag tgccaacaac
    39241 acgatcaata cagcgatact ttcttttaaa gaaatatttt tattcatcgt cgatctccta
    39301 tttcaaatga aaaggccctg ccatgaaaaa actattcact tggcaggacc tcctactaaa
    39361 tcagcgacaa aacgtcggct gaaagactta tttttttatt tttcttctaa acttttttct
    39421 acctcaatcc cccaagaatc tttcttgaag tgattgatga tcaatggaat aacgaacatg
    39481 accactgtga tagctgctac taataatgca tcactcaatg tcgctgtacc taatgtagca
    39541 aatacgacaa cgatgataga gaagatcagc atgcagctga ctacccaagc taatccgttt
    39601 ccgtttttac caattcggta aggacgttcg aattctggat gtttcttacg cattttaagc
    39661 aatgaaacag caatcaaaat gtacacgata caataaataa cagttgtagc atttgtaagt
    39721 gtcaagaata cagcacttac gtcatccatt acaccataaa gcaaagcaaa taatgaaata
    39781 acgattgatt gtgttagaac gacatttcta gatacgccgt atttgttttc tttatggaat
    39841 ccgaattttg gtggaaggaa tccttcacga gctacttgga tgattgtttt tgatggtcca
    39901 gtaacccaag ctgataattg taaaagcaca ccaataaata ccatgaagct gaagatattt
    39961 ccgatcactt ctggtaagcc taggatttga cagtcaatca agataggctg cgtgatattt
    40021 gctaattcca tttttccatc tggtacgaca tttgctacaa gcatcgcatt gatgacattc
    40081 aataaaacta atccgatcaa agcgataaat acgccttttg tataattttt tgttgcgtct
    40141 ttcaaacgag gcatgtaaac agaactcatt tcgatcccaa caaaaataaa tgtgatacca
    40201 gctaagtatt tcaatgaatc aatatgttcc aagtcaggga aagcttttga ccatgaaaag
    40261 tcacctagat agccgtttga gactagtccg actttgaagg ctgccaaaac acctaaaacg
    40321 aacatgatga ctactgggat atatacccct agccatacac cgatgttccc gccgactttt
    40381 gccatatcga atttcaagtt caagatcgtg atgatccagt agataactaa aatacaacct
    40441 aaaacgaacc agtgattatt gccaagttct acattaccaa atgtgtttcc taacaatggt
    40501 cctaaagtag aagcgaccat taccatacct gggaacattt gcacccataa tagccatgca
    40561 acgacaaatc cccatttgct gcccagtgct gtttttaccc ataactgagg accgccttct
    40621 tgaggaagca tcgtggaaag ctcaccggaa accattgata tcggaccggc aaaaaacaaa
    40681 actgcaaaaa ttgaataaaa aatcaatgtc caaccgactg cggctaacgt aggaatactc
    40741 cgaaccgttg cacacagtgc cattgtcaga ccgataaagg tcccaagacc cattttactt
    40801 acttcagatt tactcaccgt tgacactctc cttgattttt tggataaaat aacggtgtct
    40861 ttgaagtagt ttcagcaata aacgaaacaa ttcagcgaat atttgagggg attctttgag
    40921 tttattgctt gaagggtttc ttcaaagaca acctcaaaac aaagggatcg aagagcttag
    40981 gtctcccctt gctcttcaga tccaaacatg cgtcagaaac agttagtatt caagctgttt
    41041 tctaacatga ttaccttgct tattttgctt cgcttgccaa taattggtct gcgtagattt
    41101 tttccaattt ttcttgaaga gctgctttga ttttttcagc atattcttca aagtgttctt
    41161 ctttgttcat gtatggtgtc attacgctgg cacgcaatac agttactttt ccggcacggt
    41221 tccattcttc atctgagaat cctagttggt tcacaaattg taatgggctg ttgccatagt
    41281 ctgggatagc aaagtcagta tgtgaagtta agaactcatt accgtaaatg cttcctttca
    41341 cgtatgaaga ataatcatat acgtcatggt tcaatttatt cattgcaact aagtcatcgt
    41401 tgcctttttc tttgaataca tagtctacca tattgaaatc tggataagtt aatggatgaa
    41461 cttcgatttc tttgtctcct actttaaatg acaagtcatt caagaagttg tagaaacgat
    41521 gtgcaccttc gattgatgca cccattaatt taccatatcc tgtgacattc aaaggtaaga
    41581 catggtgtgc tgcccaaacg ctggctgctg ttgcccctgc ttttgaacct tccaagatat
    41641 aagccccaag taatgctggg atatctgcgc ctttttcaaa tacatatgtc gcaaagtaag
    41701 agataacgtc tctcatgcgg atatctttga tgacgatacc accggcagaa tatggaacat
    41761 agcccatttt atgtgggtca atcgttacag attctgcttc ttcgattgct ttatatgcac
    41821 tgtgtacttc ttctaagata taatctttgt tttctgtaaa gacattgtat ttgtaatgaa
    41881 catcttttaa atcttcaaat ggaatgaagt tgttgtcttc gtctaagaaa atcgcacggc
    41941 cgtatccgcc ataagcagca tctacgtgta agtagaagta aataccgtct ttttctaata
    42001 cgcgacgcaa tgcaacgatt ttatcgatac cgtcgattgc tccttcttct gttgatccta
    42061 caacaccgac tacacctagg attggtgttt tttcagctgc tagaccacga acgatttttt
    42121 caagttcgtt gatgtccatg cgatagttgt ggtcaactgg cactggaatc acttggtcta
    42181 aaccgatacc gatgatgtcg gcagctttta gccatgaata gtgtttagtt tgtggaacca
    42241 accattttcc taatttttct aaatgttttc cgctacgtgc tgagtgagct ttgatttcgt
    42301 cgattttttc tgggacgctg tctaacagat tcatgatttc ttctgttgac aagttcatta
    42361 attcccaatc gctttttcca gcaactagtt caggtgtcac ttctttcatt gcaagcggca
    42421 atgatttgat gttgcgggca taccaaagtc cttctaagtt tgctaaagaa ccatcagcaa
    42481 cgatgtggcc ccagccatct ttatagctca ttaattttgc aaattccatt cctacttctt
    42541 cttccatttg gcttgttgca ggagaagatt cataggcaac gttattacca ttccatagca
    42601 tagcaaaatt gtacgctagt aaagatggca tcaatgtttc agagttcata tgaccccagt
    42661 aacgtccggc attatgccaa ggaacggaat gggtacgcat ccgcgctgaa atttctgata
    42721 gaacatcctt tgttttattc acagtgtgtt caaaactagc actgcttttt tcttctggcg
    42781 taataatagg catgtcttga ggcatgtagt tttgacgcca gcctaaatgt tcatccacca
    42841 attcattcaa caaagcttta tagatttggc cgttctcagc tttgtcccca atgaataggg
    42901 catttaggtt tagatctttc gacaatgatt cactcatagt gaaaccctcc taaaaaaata
    42961 tttaaatttt gtcgagccct acattacacc tgttcttcta ttctgtaaac taagttttgg
    43021 cctgattttt aatcatattt taatatgtat caattatccg aattgttcac acttttgaaa
    43081 taattaaaaa aaacgaggga atttacaaat gtaaactccc tcgcaaagat tgtattaacg
    43141 acaaccagcg ttctatttta ggatgggttt caaaattgga atgttgtaat agtatttccc
    43201 tttttcaagg taaaaactta ctaaattcta atcaattact ttggccaaaa agtaattttt
    43261 cttacctttt cgaataacca caaacttacc atcgaattca tcggatggat ttatgacaaa
    43321 atttaaatcg gtaacgcggt caccgttaat acttatagct ccgtttgaaa catcttcacg
    43381 tgcttgacgt ttagatggtt caatttttgt ggaaacaagc agttccacga tattttcagg
    43441 tgtgcttgaa atttcaacgt tcggcatttt accgaatcct tgcgcgattt cttccgcatt
    43501 tagttcttta atatttccag agaacaatgc ttgcgttatt ttttgcgctt ctttcaagtc
    43561 ttcttcacta tgcacaaatc ttgtaacttc ttctgccaaa cggcgttgtg cttcacgttt
    43621 ctcaggttct gtctctactt ttttcgctaa gtcttcgatt tcttcttgag acaagaaagt
    43681 aaagaatttc aagtatttca ctacatcgcg atcatcttga ttcaaccaga attggtagaa
    43741 ctcaaatggt gaagttttct tcggatcaag ccaaacggca ccgcctgcag tttttccgaa
    43801 tttcgttccg tcagccttca gcatcaatgg gatcgttaaa ccgaacgctt ctgcttctgg
    43861 tccttctaat tttcggatta ggtctaatcc agctgtgata tttccccatt ggtctgctcc
    43921 accgatttgc aattgcacat cgtatgtttt atgcagatgc aaaaagtcga tagattgcag
    43981 gatttggtaa gtaaactctg taaatgagat tcctacttcc aaacggctgg caacgatatc
    44041 ttttgctaac atcgtgttga tgttgaagtt tttaccgtag tctctcaaaa attcaagtaa
    44101 agagattttt gacaaccagt cgtagttgtt cacaaatgtg atatttgcat ctttaccaaa
    44161 caattttctc atttgattgg aaagtgcgtc cacgttatgt tgcacttgtt ccatcgtttg
    44221 caacacacgt tctgttttgc ggccgcttgg gtcgccgatc gttcctgttc cgccgccaat
    44281 cagaatatat ggatgatggc ctgccaattg gaatcttttc atcatcataa atggaatcaa
    44341 atgtccgata tgcatcgaat caccagttgg atcaacgccg caataaagtg aaatgctctt
    44401 ttcttcaacg agctcgcgaa gtctttcttc gtttgtttgc tgattaatgg cgtcgcgcca
    44461 agttaattca tcaataatat tcattttact gacctccgtg tagttttttt atgaaagcag
    44521 gaacctgcta gcatatgtaa ttaattattt cttcaggata ggataggaaa taaaaaaagt
    44581 cccttggcat ataagccaag ggacgaaatc tcgcggtacc acccaatttg tatgtctaga
    44641 tatccaacat acctctcgaa catttaacgc tgtcgcgtgg caatcactgc caaagactcc
    44701 tgaaatgtaa ttcgctttaa agcatgtact ggtttgcagc gaccaccagc tttctgaaac
    44761 attggcttta acaactactt cgtttcgatc ttcgtcttgt tattattaat atgtgtctat
    44821 catagagcgc aactttttct ttgtcaacag aaaaaggata ttttttttaa attcctcatt
    44881 ttttcttttt tgaatccgct ctaattccga acattttatt ttcaattatt gttaaaagaa
    44941 ataacttttt ctgtattttt tctgcaattt atcgagaagt cactttactt tgcctcttat
    45001 tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg
    45061 tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt
    45121 ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct
    45181 gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact
    45241 caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa
    45301 gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa
    45361 gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg
    45421 atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg
    45481 gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta
    45541 gttaatttga atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt
    45601 gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa
    45661 tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa
    45721 aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc
    45781 gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc
    45841 ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat
    45901 gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac
    45961 ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa
    46021 gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca
    46081 aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa
    46141 aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct
    46201 tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc
    46261 gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc
    46321 aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat
    46381 gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc
    46441 ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt
    46501 tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag
    46561 aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca
    46621 agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt
    46681 tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga
    46741 taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca
    46801 atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc
    46861 agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca
    46921 gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct
    46981 gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga
    47041 gcctcccgta attttaaact tttataatat atatagttca aatttattct gaggtttatt
    47101 ataactatct attgagaaag tatcaagcta gcaattcctt gtttatgaat tca
//
LOCUS       DAZKOK010000007        46117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000007 DAZKOK010000000
VERSION     DAZKOK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.007"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..969
                     /locus_tag="ACG90W_000374"
     CDS             88..969
                     /locus_tag="ACG90W_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0476770.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            982..2592
                     /locus_tag="ACG90W_000375"
     CDS             982..2592
                     /locus_tag="ACG90W_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476771.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2669..3328)
                     /locus_tag="ACG90W_000376"
     CDS             complement(2669..3328)
                     /locus_tag="ACG90W_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0476772.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3611..4276)
                     /locus_tag="ACG90W_000377"
     CDS             complement(3611..4276)
                     /locus_tag="ACG90W_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0476773.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4423..5028)
                     /gene="dhaL"
                     /locus_tag="ACG90W_000378"
     CDS             complement(4423..5028)
                     /gene="dhaL"
                     /locus_tag="ACG90W_000378"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0476774.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5056..6066)
                     /gene="dhaK"
                     /locus_tag="ACG90W_000379"
     CDS             complement(5056..6066)
                     /gene="dhaK"
                     /locus_tag="ACG90W_000379"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0476775.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6041..6436)
                     /gene="dhaM"
                     /locus_tag="ACG90W_000380"
     CDS             complement(6041..6436)
                     /gene="dhaM"
                     /locus_tag="ACG90W_000380"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0476776.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6590..6910
                     /locus_tag="ACG90W_000381"
     CDS             6590..6910
                     /locus_tag="ACG90W_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0476777.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7022..7879)
                     /locus_tag="ACG90W_000382"
     CDS             complement(7022..7879)
                     /locus_tag="ACG90W_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0476778.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7987..9291)
                     /locus_tag="ACG90W_000383"
     CDS             complement(7987..9291)
                     /locus_tag="ACG90W_000383"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0476779.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9288..9797)
                     /locus_tag="ACG90W_000384"
     CDS             complement(9288..9797)
                     /locus_tag="ACG90W_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476780.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9972..12107)
                     /locus_tag="ACG90W_000385"
     CDS             complement(9972..12107)
                     /locus_tag="ACG90W_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0476781.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12190..13845)
                     /locus_tag="ACG90W_000386"
     CDS             complement(12190..13845)
                     /locus_tag="ACG90W_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0476782.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13847..14161)
                     /locus_tag="ACG90W_000387"
     CDS             complement(13847..14161)
                     /locus_tag="ACG90W_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0476783.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14331..14591
                     /locus_tag="ACG90W_000388"
     CDS             14331..14591
                     /locus_tag="ACG90W_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0476784.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14605..14919
                     /locus_tag="ACG90W_000389"
     CDS             14605..14919
                     /locus_tag="ACG90W_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0476785.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15649..16203)
                     /locus_tag="ACG90W_000390"
     CDS             complement(15649..16203)
                     /locus_tag="ACG90W_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0476786.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16329..18258)
                     /locus_tag="ACG90W_000391"
                     /pseudo
     CDS             complement(16329..18258)
                     /locus_tag="ACG90W_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18564..19280)
                     /locus_tag="ACG90W_000392"
     CDS             complement(18564..19280)
                     /locus_tag="ACG90W_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0476787.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19281..21104)
                     /gene="glpO"
                     /locus_tag="ACG90W_000393"
     CDS             complement(19281..21104)
                     /gene="glpO"
                     /locus_tag="ACG90W_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0476788.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21118..22614)
                     /gene="glpK"
                     /locus_tag="ACG90W_000394"
     CDS             complement(21118..22614)
                     /gene="glpK"
                     /locus_tag="ACG90W_000394"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0476789.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22750..24210)
                     /locus_tag="ACG90W_000395"
     CDS             complement(22750..24210)
                     /locus_tag="ACG90W_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0476790.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24200..25495)
                     /locus_tag="ACG90W_000396"
     CDS             complement(24200..25495)
                     /locus_tag="ACG90W_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0476791.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25827..26504)
                     /locus_tag="ACG90W_000397"
     CDS             complement(25827..26504)
                     /locus_tag="ACG90W_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0476792.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26534..27625)
                     /locus_tag="ACG90W_000398"
     CDS             complement(26534..27625)
                     /locus_tag="ACG90W_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0476793.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27691..28161)
                     /locus_tag="ACG90W_000399"
     CDS             complement(27691..28161)
                     /locus_tag="ACG90W_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0476794.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKKIKFFKQISCMYN"
     gene            complement(28745..30202)
                     /locus_tag="ACG90W_000400"
     CDS             complement(28745..30202)
                     /locus_tag="ACG90W_000400"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0476795.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30199..32094)
                     /locus_tag="ACG90W_000401"
     CDS             complement(30199..32094)
                     /locus_tag="ACG90W_000401"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0476796.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32152..33927)
                     /locus_tag="ACG90W_000402"
     CDS             complement(32152..33927)
                     /locus_tag="ACG90W_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0476797.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33977..35473)
                     /locus_tag="ACG90W_000403"
     CDS             complement(33977..35473)
                     /locus_tag="ACG90W_000403"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0476798.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35667..37289)
                     /locus_tag="ACG90W_000404"
     CDS             complement(35667..37289)
                     /locus_tag="ACG90W_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0476799.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37617..38930)
                     /locus_tag="ACG90W_000405"
     CDS             complement(37617..38930)
                     /locus_tag="ACG90W_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0476800.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39175..39399)
                     /locus_tag="ACG90W_000406"
     CDS             complement(39175..39399)
                     /locus_tag="ACG90W_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476801.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39419..39841)
                     /locus_tag="ACG90W_000407"
     CDS             complement(39419..39841)
                     /locus_tag="ACG90W_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0476802.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39983..40693)
                     /locus_tag="ACG90W_000408"
     CDS             complement(39983..40693)
                     /locus_tag="ACG90W_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0476803.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40711..41208)
                     /locus_tag="ACG90W_000409"
     CDS             complement(40711..41208)
                     /locus_tag="ACG90W_000409"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0476804.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41245..41697)
                     /locus_tag="ACG90W_000410"
     CDS             complement(41245..41697)
                     /locus_tag="ACG90W_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0476805.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41832..42437)
                     /locus_tag="ACG90W_000411"
     CDS             complement(41832..42437)
                     /locus_tag="ACG90W_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0476806.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42887..43507)
                     /locus_tag="ACG90W_000412"
     CDS             complement(42887..43507)
                     /locus_tag="ACG90W_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0476807.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43509..44378)
                     /locus_tag="ACG90W_000413"
     CDS             complement(43509..44378)
                     /locus_tag="ACG90W_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0476808.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44401..44808)
                     /locus_tag="ACG90W_000414"
     CDS             complement(44401..44808)
                     /locus_tag="ACG90W_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0476809.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44827..46116)
                     /locus_tag="ACG90W_000415"
     CDS             complement(44827..46116)
                     /locus_tag="ACG90W_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0476810.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12947 a         9841 c         7893 g        15436 t
ORIGIN      
        1 aaagttttat aaaaaggtat aaaacctttt tattaaacta tttaatcttg tttagtatat
       61 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
      121 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
      181 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
      241 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
      301 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
      361 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
      421 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
      481 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
      541 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
      601 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
      661 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa
      721 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
      781 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
      841 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
      901 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
      961 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
     1021 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
     1081 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
     1141 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
     1201 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
     1261 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
     1321 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
     1381 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
     1441 tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
     1501 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
     1561 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
     1621 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
     1681 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
     1741 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
     1801 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
     1861 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
     1921 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
     1981 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc
     2041 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
     2101 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
     2161 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
     2221 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
     2281 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
     2341 ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt
     2401 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
     2461 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
     2521 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
     2581 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
     2641 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
     2701 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
     2761 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
     2821 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
     2881 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
     2941 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
     3001 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
     3061 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
     3121 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
     3181 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
     3241 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
     3301 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
     3361 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
     3421 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
     3481 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
     3541 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
     3601 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
     3661 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
     3721 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
     3781 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
     3841 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
     3901 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
     3961 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
     4021 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
     4081 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
     4141 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
     4201 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
     4261 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
     4321 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
     4381 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
     4441 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
     4501 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
     4561 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
     4621 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
     4681 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
     4741 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
     4801 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
     4861 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
     4921 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
     4981 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
     5041 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
     5101 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
     5161 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
     5221 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
     5281 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
     5341 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
     5401 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
     5461 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
     5521 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
     5581 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
     5641 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
     5701 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
     5761 atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
     5821 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
     5881 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
     5941 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
     6001 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
     6061 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
     6121 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
     6181 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
     6241 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
     6301 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
     6361 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
     6421 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
     6481 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta
     6541 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
     6601 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
     6661 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
     6721 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
     6781 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
     6841 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
     6901 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
     6961 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
     7021 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
     7081 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
     7141 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
     7201 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
     7261 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
     7321 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
     7381 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
     7441 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
     7501 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
     7561 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
     7621 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
     7681 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
     7741 tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt tcagaagttt
     7801 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
     7861 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
     7921 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
     7981 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
     8041 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
     8101 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
     8161 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
     8221 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
     8281 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
     8341 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
     8401 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc gatatagcat
     8461 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
     8521 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
     8581 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
     8641 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
     8701 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
     8761 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
     8821 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
     8881 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
     8941 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
     9001 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtata
     9061 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
     9121 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
     9181 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa aatacataat
     9241 agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca tttcagttcc
     9301 accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt ctcttcggag
     9361 acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc cgtatacact
     9421 tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa agatttattt
     9481 acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt aatcaggaaa
     9541 ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt ttctgataaa
     9601 aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa aaagacccat
     9661 ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg ccaaatcttt
     9721 aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc aacttcttct
     9781 cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag gagtttacca
     9841 tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa
     9901 catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc tttttatttt
     9961 ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc tctagatatc
    10021 gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa tggatataag
    10081 attacatgat caaattccag tccttttgct agactcaaag gaatgatttg gcagttttct
    10141 aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc ttctgtttgg
    10201 gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag tattttcaac
    10261 aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg tcggatcgat
    10321 acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt gattgctccg
    10381 ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta gtcgtcgaaa
    10441 caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct
    10501 actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg tgccagggta
    10561 taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac cttttcaaca
    10621 agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt gggttgccaa
    10681 gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg ctcatccacc
    10741 catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa aagtttcctt
    10801 gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa taattccaac
    10861 tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt tgcttgtttc
    10921 aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag cctatcgatc
    10981 aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt agaaaggact
    11041 gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa caaaggatct
    11101 gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg ctccgataca
    11161 ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc tgctgaaagc
    11221 tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa tgatggtagg
    11281 acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa gtcatcggat
    11341 gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat agcagaggtt
    11401 ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc ccgaatgatt
    11461 gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt ctcatcttgg
    11521 cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa aaacttgacc
    11581 aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc gattttattt
    11641 tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc ggcaattggt
    11701 gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc tgtaccgata
    11761 taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc aaagtaggga
    11821 cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg tttgatattc
    11881 atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag gttatctaag
    11941 taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg gtttccttct
    12001 atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag cttttgatac
    12061 acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca cattctcctc
    12121 tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat gcaagctttt
    12181 taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt gatataaaga
    12241 aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc tttccgctag
    12301 atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt ctaattcatt
    12361 taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt tgcccttttc
    12421 caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt ctgtcggcaa
    12481 ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg catatcctag
    12541 catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg
    12601 taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga tccgtttatc
    12661 cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg gatggaagat
    12721 caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt ttcctgatac
    12781 atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta aaccggtgga
    12841 tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt gatttcttcg
    12901 agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg gtgaagcaag
    12961 agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat cccccaccgc
    13021 ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc cgcgtatgcc
    13081 taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa tggataaaat
    13141 aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc
    13201 aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca attctttttc
    13261 tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt gttcgatcac
    13321 catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga tgaatcctgc
    13381 accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt tatcgatatc
    13441 tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa tcgatggaac
    13501 aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc
    13561 ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt catgatttgg
    13621 acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa gaagattttc
    13681 ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa aagaaacata
    13741 cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa gccgtgtagc
    13801 agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat ttccctccta
    13861 ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca taaccagatt
    13921 tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc acatagacgg
    13981 tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt ttagctcctt
    14041 ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct ggattttcca
    14101 aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat agcaaattca
    14161 ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa acaggagctt
    14221 tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat atttgcataa
    14281 aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt atggagattg
    14341 ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa
    14401 aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt
    14461 ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga
    14521 aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc aagatgatta
    14581 ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga attagaaaaa
    14641 tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga aacgacgatt
    14701 gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa tgcagaattc
    14761 ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt
    14821 tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct tgatttagtt
    14881 gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt tgcgacagaa
    14941 gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg
    15001 aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca aacaaaaata
    15061 gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    15121 tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa aaaacgggaa
    15181 aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    15241 cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
    15301 aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
    15361 taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag agaaacaaac
    15421 ggaaaaatag ctgctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    15481 aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt
    15541 tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag tagctactgg
    15601 gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg
    15661 aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat
    15721 gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat
    15781 catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt
    15841 gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa
    15901 tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag
    15961 ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata
    16021 tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc
    16081 tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg
    16141 gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt
    16201 cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat
    16261 aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta tgagctaatt
    16321 tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt
    16381 tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta
    16441 cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct
    16501 gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat
    16561 acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg
    16621 acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc cttcctattt
    16681 gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag
    16741 taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa
    16801 gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa gtgcttcccg
    16861 atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc
    16921 tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc
    16981 gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac
    17041 caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa
    17101 tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa
    17161 gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt
    17221 caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat
    17281 caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa
    17341 tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc
    17401 attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc
    17461 gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac
    17521 ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga
    17581 aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc
    17641 atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac
    17701 aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt
    17761 tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga
    17821 cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt ctacatttat
    17881 caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc
    17941 aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt
    18001 tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc
    18061 cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg
    18121 acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct
    18181 atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact
    18241 aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat
    18301 aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt
    18361 attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa
    18421 ctttgtttat tggctattcg acttactgat tagataccag ctacactcac aaaacatacc
    18481 ccattcttct aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc
    18541 aaactttagt atgttttata gatttacacc attaatgaat agacacctac cgcgataatt
    18601 gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta
    18661 ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt
    18721 gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg
    18781 aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat
    18841 acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct
    18901 gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa
    18961 gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga
    19021 acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc
    19081 aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa
    19141 ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc
    19201 gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc ccccatgatt
    19261 tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca
    19321 atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat
    19381 ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac
    19441 agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa
    19501 ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt
    19561 gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct
    19621 tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat
    19681 ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc
    19741 tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc
    19801 ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct
    19861 aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg
    19921 ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta
    19981 tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca
    20041 gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc
    20101 aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct
    20161 tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag
    20221 taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg
    20281 aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta
    20341 gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc
    20401 caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc
    20461 aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc
    20521 attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa
    20581 cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc
    20641 aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt
    20701 cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg
    20761 aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg
    20821 tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca
    20881 tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc
    20941 ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg
    21001 actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc
    21061 tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta
    21121 ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg
    21181 ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc
    21241 tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt
    21301 ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag
    21361 atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc
    21421 atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt
    21481 aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg
    21541 atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct
    21601 tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc
    21661 tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac
    21721 ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt
    21781 ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg
    21841 ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg
    21901 tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt
    21961 gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc
    22021 tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc
    22081 ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc
    22141 tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc
    22201 tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc
    22261 attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata
    22321 gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc
    22381 aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga
    22441 ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata
    22501 ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc
    22561 tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat
    22621 tccctccatt atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa
    22681 aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct
    22741 attttttaat caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat
    22801 gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca
    22861 aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc
    22921 gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact
    22981 ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac
    23041 attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa
    23101 ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat
    23161 tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa
    23221 aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt
    23281 caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc
    23341 ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat
    23401 cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc
    23461 tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg
    23521 atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt
    23581 tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc
    23641 taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag
    23701 cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag
    23761 ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt
    23821 caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg
    23881 aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc
    23941 ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc
    24001 ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat
    24061 atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt
    24121 gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc
    24181 ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca
    24241 tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg
    24301 tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc
    24361 tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt
    24421 tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat
    24481 aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat
    24541 tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga
    24601 catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg
    24661 gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca
    24721 agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct
    24781 gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt
    24841 ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa
    24901 gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat
    24961 aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca
    25021 acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat
    25081 tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg
    25141 ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat
    25201 caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg
    25261 tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag
    25321 aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc
    25381 atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa
    25441 gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca
    25501 ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat
    25561 aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac
    25621 cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc
    25681 tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc
    25741 tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat
    25801 agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct
    25861 aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact
    25921 tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct
    25981 aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc
    26041 actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca
    26101 acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc
    26161 ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc
    26221 gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt
    26281 ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc
    26341 ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt
    26401 actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc
    26461 ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc
    26521 tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat
    26581 aaagctcttt atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca
    26641 ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg
    26701 catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag
    26761 ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat
    26821 cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct agttttgact
    26881 cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt
    26941 cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg
    27001 agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc
    27061 attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg
    27121 ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa
    27181 atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag
    27241 gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac
    27301 ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg
    27361 ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg
    27421 gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt
    27481 tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga
    27541 gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag
    27601 taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta
    27661 tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa
    27721 aaattttatt tttttttgta acttatctgt tgcatcattc attgtctggg ctaattcttt
    27781 acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc agctaccatc
    27841 gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca agcgaccttc
    27901 gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag caatcgttga
    27961 tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt tttcttttaa
    28021 tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg cttcatcagc
    28081 taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt catctaacgc
    28141 tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat agtatgattt
    28201 ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat ttgaaaaaaa
    28261 gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc tggaacaccg
    28321 tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat tttgtgaaaa
    28381 tggctttcca catttttgca taaatttggc ttaataccga aaaactggct tttgaacacc
    28441 gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac agccaaacaa
    28501 aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc tgattgtaca
    28561 ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga gcaatttcaa
    28621 aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt ggcaggagaa
    28681 tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc cttatttcct
    28741 tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca aatgattttt
    28801 tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag atatatccat
    28861 aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc cacgacgtat
    28921 aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata tgctgcttca
    28981 aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg tctatagctc
    29041 cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat tgattcaaag
    29101 caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga agatacggat
    29161 ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct ggatgagcac
    29221 tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag gctgctaaga
    29281 tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac attcgttgcg
    29341 tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa gttctttgct
    29401 gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga agctgatagc
    29461 tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt gtagctaaag
    29521 cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt
    29581 tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg atctcattga
    29641 aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca gcgaaacgaa
    29701 cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc aaatataacg
    29761 gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga cattcgtcaa
    29821 acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca ccatgaggaa
    29881 aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca gcaaataaac
    29941 ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga taataatact
    30001 tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc cattgatctg
    30061 tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca gataatcctt
    30121 tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg ccccataaaa
    30181 atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc ttgtttgatt
    30241 atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa caataaccat
    30301 tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca tcaataaatc
    30361 gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc catttaaatt
    30421 cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga ctaccccaat
    30481 tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acagaactgt agatttttcc
    30541 gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg ttggatcgtt
    30601 tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta tatcctttga
    30661 ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac aggaactgat
    30721 catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat aaatcgggaa
    30781 agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac cagctactcc
    30841 accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt taagagtcac
    30901 accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag ataaagataa
    30961 actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc ctgcttgcgc
    31021 agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag aaagattgtt
    31081 ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt gccaaaatcc
    31141 gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat atccagctgc
    31201 aatcagctga cttgcataat ctgcaattgg acctacaact aataaaataa ttgggaccat
    31261 cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca agcttttgcg
    31321 aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa taaccgtact
    31381 tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc tatcactata
    31441 tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga tgaaacgatc
    31501 tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat agaaaaatgc
    31561 atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata aattcaaact
    31621 cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc ctaaagtcgg
    31681 ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt tctcagttcc
    31741 tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata cgtcatacac
    31801 ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga ctacagtgat
    31861 cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt ttagggtaaa
    31921 gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg
    31981 gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt taattaatgc
    32041 cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct ccattcaatt
    32101 agtacccctt ctaatagcga acgaaactgt acaatagtgt gtatggttta cttagccgag
    32161 caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt caaaggagac
    32221 aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa gctgatactc
    32281 tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct cggcatttaa
    32341 cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat ctatcttttt
    32401 atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg ttttcttttt
    32461 ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag attcaaactc
    32521 atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct cttgctcagt
    32581 agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa gatacactgg
    32641 ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt tttttagtgt
    32701 attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt ttcgaaaagg
    32761 attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa
    32821 ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat aacgggagat
    32881 gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg ttttcatttg
    32941 tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag gaagatctaa
    33001 aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt tgtttttttc
    33061 atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt gtttttcttg
    33121 attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct ctaccatcgg
    33181 aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa gaatggtcaa
    33241 aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat tatatgatat
    33301 aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa ttgtactgat
    33361 ttgttgagga aaccttgcgt gttggatcac cgtttctgtt ccaacaagag ataagaaaat
    33421 tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa cttccagttc
    33481 taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg atacggctgt
    33541 ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt tttgaaacaa
    33601 taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt tatgtttcgt
    33661 cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa aataacctga
    33721 acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat ttttgatttc
    33781 agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc ttgttatcca
    33841 atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta agtccatttg
    33901 cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac aatagtcact
    33961 tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta ccattagact
    34021 ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag cttccgctcc
    34081 catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc cctgttcctg
    34141 ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa ccatctttta
    34201 ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt ggatcgtgga
    34261 ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact atgaataatg
    34321 gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga ggatcaattg
    34381 gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa ccatttgctc
    34441 cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga taataactaa
    34501 atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct aacattactg
    34561 gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc cctcggcaca
    34621 tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat acattctctg
    34681 gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata cccatcttag
    34741 catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga aacatatgat
    34801 gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt ttatctctag
    34861 cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta ttgatctcat
    34921 taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc tgcgcataaa
    34981 attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct accaatgcta
    35041 agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga tttaatgtat
    35101 tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg tctcctcttg
    35161 gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac tccttcagta
    35221 aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa agataatgga
    35281 gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga atactaggat
    35341 gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg tcttccaacc
    35401 atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt tctggaaata
    35461 tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga agtatagctc
    35521 gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa aattgaatgc
    35581 tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc gaaagaaaac
    35641 actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc ttcataaggt
    35701 cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac aataaccctt
    35761 aaagcttcac gtggaatagt gaacgtctgc ttttcactgg acatattaca aataacgagc
    35821 cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt cttttccact
    35881 aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat caatttcttg
    35941 tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat ttctttataa
    36001 tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc acgggcgttc
    36061 cattgcatag gcgtcctagc attgtctctt cctttcgcct tgattgattc caagatttcc
    36121 tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc aatatctttt
    36181 gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc ttgatagata
    36241 taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact ttcttttcga
    36301 tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt attccaaaat
    36361 aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac ttttttcagt
    36421 tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg atctaatcct
    36481 atgtgttcga attggaagac catggataat tcatttcttt taggattgga ataaagttta
    36541 gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa
    36601 gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact tgtgatttct
    36661 ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc aataccttta
    36721 tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt tttccaattc
    36781 aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt ttcatccaac
    36841 tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc attgaccgga
    36901 tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc ctcaatgaac
    36961 cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg gatatttctt
    37021 ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc tgccatgatt
    37081 gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta tactggagaa
    37141 agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat aatcccccgc
    37201 aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat ttgataaaca
    37261 actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga tgtttctatt
    37321 ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt tcaaatagct
    37381 attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa attccctcaa
    37441 ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa cttctttata
    37501 aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa tataagaaac
    37561 tattttttcg tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat
    37621 ttcacattca atatccacat gaatcccaca aatacaaaga acagaccata catgattgga
    37681 gatacttctt ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat
    37741 gcaatcccat ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg
    37801 atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct
    37861 accataatca atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag
    37921 aacaagccta acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca
    37981 acggcgatac cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa
    38041 cccgcaagca tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg
    38101 tctttcatga aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa
    38161 gtcacaagca agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca
    38221 tgttttaatg caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa
    38281 ggcattggaa tcaaacctgt gaccagacct aaaatcgttg tagctgccat cccgatgaag
    38341 atgccacctg gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc
    38401 aaccaagtag aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg
    38461 atgatcccac cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc
    38521 gcatatttca aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg
    38581 atcaaaataa aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc
    38641 cccattccta aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt
    38701 ccaattggat aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca
    38761 gtcgcagtaa acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca
    38821 aataagatat aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc
    38881 gtggtgttca atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc
    38941 cctaaatccg aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg
    39001 tgttttatca tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa
    39061 tccgaacgtt ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga
    39121 ttacaagaat ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata
    39181 gtgtgatttg attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac
    39241 aggattcaga ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt
    39301 cttctttttt cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg
    39361 atacttcaaa taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc
    39421 aatcagacga ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga
    39481 aagtcaagat gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag
    39541 tttcaaattc atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa
    39601 acttctgcac aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta
    39661 caatactctt aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt
    39721 ctacaaagaa tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt
    39781 gtatcatcat ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca
    39841 ttatatcacg aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata
    39901 gtttctagga ttgctttcct ccacaaactt tttttacaaa aaaaaaacgg caatgaatgc
    39961 cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt
    40021 gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct
    40081 gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg
    40141 tttcttccgt cagcatcgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc
    40201 cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta
    40261 aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt
    40321 cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta
    40381 caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa
    40441 tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa
    40501 tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct
    40561 cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat
    40621 ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta
    40681 ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc
    40741 tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt
    40801 acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt
    40861 ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg
    40921 tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc
    40981 agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac
    41041 taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc
    41101 ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa
    41161 tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc
    41221 gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt
    41281 ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc
    41341 ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa
    41401 aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt
    41461 atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt
    41521 tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag gattttcgca
    41581 aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt
    41641 gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc
    41701 atcccacctt tattttgttt gtaagactag ttcacctctt tttgttgcaa atgcaactat
    41761 tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt
    41821 tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc
    41881 ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg
    41941 gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca
    42001 agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct
    42061 cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt
    42121 tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt
    42181 ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt
    42241 ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt
    42301 cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca
    42361 aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga
    42421 gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa
    42481 gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag
    42541 aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc
    42601 gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt
    42661 cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa
    42721 cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct
    42781 gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt
    42841 aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat
    42901 tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta
    42961 catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct
    43021 ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac
    43081 catagtgata atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt
    43141 accgacgttg ccataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc
    43201 cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg
    43261 acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat
    43321 aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg
    43381 catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta
    43441 gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact
    43501 ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc
    43561 ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac
    43621 ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc
    43681 tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct
    43741 ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc
    43801 agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca
    43861 atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta
    43921 tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt
    43981 tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg
    44041 atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg
    44101 attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc
    44161 atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta
    44221 gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc
    44281 atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct
    44341 tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt
    44401 ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc
    44461 catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt
    44521 atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt
    44581 ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg
    44641 ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg
    44701 tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc
    44761 cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac
    44821 tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc
    44881 tactgtatct acatcggttg ctgtagcatt gttgaatccc atcgaattaa agatcgttac
    44941 tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc
    45001 tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt
    45061 tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat
    45121 cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa
    45181 aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa
    45241 tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat acacacccac
    45301 tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc
    45361 agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac
    45421 catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc
    45481 gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa
    45541 gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat
    45601 gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac
    45661 caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc
    45721 catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa
    45781 aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa
    45841 atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa
    45901 tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc
    45961 actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga
    46021 tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt
    46081 caaaatattt tcgatgataa aattgataac gcccatg
//
LOCUS       DAZKOK010000008        45846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000008 DAZKOK010000000
VERSION     DAZKOK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.008"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..1121
                     /locus_tag="ACG90W_000416"
     CDS             51..1121
                     /locus_tag="ACG90W_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476811.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1136..1804
                     /locus_tag="ACG90W_000417"
     CDS             1136..1804
                     /locus_tag="ACG90W_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0476812.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1824..2402
                     /locus_tag="ACG90W_000418"
     CDS             1824..2402
                     /locus_tag="ACG90W_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0476813.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2803..3714
                     /locus_tag="ACG90W_000419"
     CDS             2803..3714
                     /locus_tag="ACG90W_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0476814.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4092..5009)
                     /gene="rnhC"
                     /locus_tag="ACG90W_000420"
     CDS             complement(4092..5009)
                     /gene="rnhC"
                     /locus_tag="ACG90W_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0476815.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5211..5648
                     /gene="zapA"
                     /locus_tag="ACG90W_000421"
     CDS             5211..5648
                     /gene="zapA"
                     /locus_tag="ACG90W_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0476816.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5706..6254
                     /locus_tag="ACG90W_000422"
     CDS             5706..6254
                     /locus_tag="ACG90W_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0476817.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6369..8729
                     /locus_tag="ACG90W_000423"
     CDS             6369..8729
                     /locus_tag="ACG90W_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0476818.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8816..9130
                     /gene="trxA"
                     /locus_tag="ACG90W_000424"
     CDS             8816..9130
                     /gene="trxA"
                     /locus_tag="ACG90W_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0476819.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9312..11090
                     /gene="uvrC"
                     /locus_tag="ACG90W_000425"
     CDS             9312..11090
                     /gene="uvrC"
                     /locus_tag="ACG90W_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0476820.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11235..12134
                     /locus_tag="ACG90W_000426"
     CDS             11235..12134
                     /locus_tag="ACG90W_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0476821.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12127..13389
                     /locus_tag="ACG90W_000427"
     CDS             12127..13389
                     /locus_tag="ACG90W_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476822.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13550..15448
                     /locus_tag="ACG90W_000428"
     CDS             13550..15448
                     /locus_tag="ACG90W_000428"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0476823.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15778..16497)
                     /locus_tag="ACG90W_000429"
     CDS             complement(15778..16497)
                     /locus_tag="ACG90W_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0476824.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16687..16734
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16834..17166
                     /locus_tag="ACG90W_000430"
     CDS             16834..17166
                     /locus_tag="ACG90W_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476825.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17153..19156
                     /locus_tag="ACG90W_000431"
     CDS             17153..19156
                     /locus_tag="ACG90W_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0476826.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19175..19645
                     /locus_tag="ACG90W_000432"
     CDS             19175..19645
                     /locus_tag="ACG90W_000432"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0476827.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19666..20250
                     /locus_tag="ACG90W_000433"
     CDS             19666..20250
                     /locus_tag="ACG90W_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0476828.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20265..21251
                     /locus_tag="ACG90W_000434"
     CDS             20265..21251
                     /locus_tag="ACG90W_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0476829.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21241..21552
                     /locus_tag="ACG90W_000435"
     CDS             21241..21552
                     /locus_tag="ACG90W_000435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0476830.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21574..23355
                     /locus_tag="ACG90W_000436"
     CDS             21574..23355
                     /locus_tag="ACG90W_000436"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0476831.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23348..24724
                     /locus_tag="ACG90W_000437"
     CDS             23348..24724
                     /locus_tag="ACG90W_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0476832.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24731..25366
                     /locus_tag="ACG90W_000438"
     CDS             24731..25366
                     /locus_tag="ACG90W_000438"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0476833.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25396..26751
                     /locus_tag="ACG90W_000439"
     CDS             25396..26751
                     /locus_tag="ACG90W_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0476834.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26943..28337)
                     /locus_tag="ACG90W_000440"
     CDS             complement(26943..28337)
                     /locus_tag="ACG90W_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0476835.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28429..29400
                     /locus_tag="ACG90W_000441"
     CDS             28429..29400
                     /locus_tag="ACG90W_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0476836.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29397..30179
                     /locus_tag="ACG90W_000442"
     CDS             29397..30179
                     /locus_tag="ACG90W_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0476837.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30348..30719)
                     /locus_tag="ACG90W_000443"
     CDS             complement(30348..30719)
                     /locus_tag="ACG90W_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0476838.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30853..31296
                     /locus_tag="ACG90W_000444"
     CDS             30853..31296
                     /locus_tag="ACG90W_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0476839.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31339..32148)
                     /gene="yidA"
                     /locus_tag="ACG90W_000445"
     CDS             complement(31339..32148)
                     /gene="yidA"
                     /locus_tag="ACG90W_000445"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0476840.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32167..33534)
                     /locus_tag="ACG90W_000446"
     CDS             complement(32167..33534)
                     /locus_tag="ACG90W_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0476841.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33518..34348)
                     /locus_tag="ACG90W_000447"
     CDS             complement(33518..34348)
                     /locus_tag="ACG90W_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0476842.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34511..34948
                     /locus_tag="ACG90W_000448"
     CDS             34511..34948
                     /locus_tag="ACG90W_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0476843.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            35024..35644
                     /gene="rpoE"
                     /locus_tag="ACG90W_000449"
     CDS             35024..35644
                     /gene="rpoE"
                     /locus_tag="ACG90W_000449"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0476844.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35833..36339
                     /locus_tag="ACG90W_000450"
     CDS             35833..36339
                     /locus_tag="ACG90W_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0476845.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36356..37255
                     /locus_tag="ACG90W_000451"
     CDS             36356..37255
                     /locus_tag="ACG90W_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0476846.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37342..38226
                     /locus_tag="ACG90W_000452"
     CDS             37342..38226
                     /locus_tag="ACG90W_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0476847.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38418..38489)
                     /locus_tag="ACG90W_000453"
     tRNA            complement(38418..38489)
                     /locus_tag="ACG90W_000453"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38454..38456),aa:Arg,seq:ccg)
     gene            38549..39883
                     /gene="rpoN"
                     /locus_tag="ACG90W_000454"
     CDS             38549..39883
                     /gene="rpoN"
                     /locus_tag="ACG90W_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0476848.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            40032..41123
                     /locus_tag="ACG90W_000455"
     CDS             40032..41123
                     /locus_tag="ACG90W_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0476849.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            41164..42165
                     /gene="gap"
                     /locus_tag="ACG90W_000456"
     CDS             41164..42165
                     /gene="gap"
                     /locus_tag="ACG90W_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0476850.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            42275..43465
                     /gene="pgk"
                     /locus_tag="ACG90W_000457"
     CDS             42275..43465
                     /gene="pgk"
                     /locus_tag="ACG90W_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0476851.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43569..44324
                     /gene="tpiA"
                     /locus_tag="ACG90W_000458"
     CDS             43569..44324
                     /gene="tpiA"
                     /locus_tag="ACG90W_000458"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0476852.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44440..45738
                     /gene="eno"
                     /locus_tag="ACG90W_000459"
     CDS             44440..45738
                     /gene="eno"
                     /locus_tag="ACG90W_000459"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0476853.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        15209 a         7928 c         9781 g        12928 t
ORIGIN      
        1 caagtgataa aattgttgta taaaatgatt atttcaggag gaggagttaa atgtttttag
       61 cattaaatga aattatgcat tcaaagttac gttatgcgtt agttgcaggg gtgatgtttt
      121 tgatcgccta cctggtattt tttttgactg ggttagctta tggcttagct caagataatc
      181 gaacagcagt cgacaagtgg gaggcagatt cgattgtgct ttcgaaagat gcaaattcaa
      241 atctagggat gtccatgatt actaagaaga tagcagaaga agtcgaaggt gggaaagtgg
      301 cgtatcttgc ccaaacacct ggagtcgtga ctagtaaaga cagtacagaa gaaggaaaaa
      361 tcaatgttag cttcttcggt atcgataaaa atcaattcat catgcccaat cttgttgaag
      421 gaaaagcttt tgataacgat gatgaggcag taggcgatat tagtttgaaa gaagaatatg
      481 gtctagccat aggagataca gttaagttat ctggtagtga caagacgttt aaactaactg
      541 gctttacgga tcatgccaaa ttcaatgtct caccagttct ctatacaacc atcaatgctt
      601 accaagaaat ccgcttcgaa aaagaagata caagtgaaaa tgcacggatt aacgcaatag
      661 ttgtcagagg taaaatcagt aatttacctg aagatttaga acagatcaaa atttctaaat
      721 tcatcaatga attacctgga tataatgcgc aagtattgac ttttggattt atgatcggat
      781 ttttgatcgt tattgcggct attgtgatcg ggattttcat ttatgtactg acgatgcaaa
      841 agattaatat ctttggtgta atgaaagcac aagggatcac aggaggattt attgcgcgtt
      901 ctgtagtggc acaaacattc attttatcat ttgttggtat tcttcttggt ctattaggaa
      961 cagtgggaac atcccttgtt ctacctgatg cggttccatt ccaaagcaat tggctgtttt
     1021 ttggcgtcat tagcttgttg atgttagtag tagctgtgtt aggcgcacta ttttctgttc
     1081 ggacaatcgt aaaaatcgat ccgttgaagg caattggata agaaggagga agaacatgaa
     1141 agcaatagaa tttaaagaag tagaaaaaag tttccaagat ggcgaccaag tagttaaagc
     1201 attgaaagaa acaaccatga gtatcgataa aggggaattt gttgcaatca tcggtccttc
     1261 tggttcaggt aaaagtactt ttttgacttt ggcaggcgga ttgcagacgc ctagcagtgg
     1321 ctctgtattt atcaatgatc aagaattcag taatcaaaaa gaaagcgaac gcgttaaact
     1381 acgttttaaa gaaattggct ttattttgca agcttccaat ctggttcctt ttctgacagt
     1441 aaaaaagcag ttattattag tagataagat taaacatgaa catcgtcaaa aagaagcaaa
     1501 cgaattattt gagcagcttg gcgtggataa gttgatcaac aagtatccag aagaactatc
     1561 tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg tacaatgatc cgtccattat
     1621 tctagcagat gaaccaactg ctagtttgga ttcagaaaag gcacgagaag tagtcgatat
     1681 cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc atggttactc acgatacacg
     1741 gctgattgac aaatgcgata gaatatttat gatggaagat ggtgtattga aagaaaaaga
     1801 ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa caaagataca cagcaaaaaa
     1861 ttttgaaaga agcgactgac ttgtttatgt ccaagggata tctcggcact tcaacaagag
     1921 aaatcgcaca aaaagttggg atcacccagc ccaatctcta tcattatttc ggtgataaag
     1981 aaaaactata tacggcagtt ttagaaaacc acttaaaaga tgttggaaaa gctttgagag
     2041 aaatcgttca aacaagcgaa acgggatttc aagtaacctt gacaaaaatg gcgcagtttt
     2101 tgattgagac acatctggtc gatttgtttc tgatgcttca cgatcttgaa agcaatcttt
     2161 caaaggaaac aagagatcat cttttctttc tatggaagaa aaattatcgt gaaccttttg
     2221 aagaaatctt ttcagcaaac caatctgtgc taagaaatgg gatttcaaaa gaaatcgcag
     2281 caaggcattt tttcttagtg ttagcacctt acatcactcg gtcgaaaaga cctgtggaag
     2341 aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa tggtgtatta ggagaagcgt
     2401 aaagctgtta tatagggaga aaatagttag gtgaagaagc aagatatgac gaagatcgtt
     2461 atatcttgct tctttatata ggtagttttt cataaataca aatttgttat tctctcatga
     2521 ttatcagggt atatttttat ttattatgtc ggtgttccag gaagtgtttt aatttaataa
     2581 cgaagaaatt aattgtttgt agaatgaagg aaactattta ttgggtagat gaatcaccac
     2641 gaatagtcaa aaggagattt caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa
     2701 tgttattttc tacggttata tcttttacag cccctttttt gcttacttaa gctgatttta
     2761 agttttggcc cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa aataattgga
     2821 gcttttgtat ttgcaatcat tatcattatt atctgttttc aaatagatgt ttttggtcaa
     2881 aaaaagatag ttggacaaac ggtcagcagt acgaacagta cggttacaaa gacaaaggaa
     2941 tcatttgatg atacagaaca agagacatta atcaaaatcg gaaaaaatga ccgtacacta
     3001 tatacagatg actcgctaac taccccactt gcaactataa acggcggaga gctggcggag
     3061 tttctttctg aaacaaaaaa tgcttatcag atcaagacaa atgatggcta tactggatat
     3121 ttagccctcg aagatggaac caaagtcaca aagaatatac aaacaaaacc ggaagagcta
     3181 tctgatgcag tgattgtatt agatcctggg catggcggaa atgatactgg ggcattaagc
     3241 aatgatgaac aaacagaaga aaaagatata acattaagta cagccataaa agtaaaaaag
     3301 gcgctagagg atgctggagc aacggtttat ttgacccata ctacagatga attagtacag
     3361 cttggagata tatgcgacta cagtgaagaa aaaaaggcag atgtctttat cagtttacac
     3421 gcagactcta cggaatatgc gaatgaagca actggaatca cgacatatta ttactatgga
     3481 caagaggaaa cattagcaca gacaattgct gactccttca ctgatctgcc tctggattca
     3541 agaggaatct ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc ttctatttta
     3601 atcgagatgg gttatatgaa taatgatagt gatttaaagg aattagtaac agacagttat
     3661 cagcagcaga ttgctgcaag tttgacacaa gcattgacca cttattttca gtaataaata
     3721 agcccagcca aacaaaaata gctcctcata tttttcgttt ggccgggctt attatcgcag
     3781 atgcaagctt ttaaacaccg tttaaggaag aggtcgtgag cctgccgttc aactctgagt
     3841 cacaaggagc aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc
     3901 gaggagttgg catgagaaca ccgtttaaga aaaaggttgt gaaatcaacg ttttgttctg
     3961 agtattagag aaacaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4021 ccgaggatca gatgattgaa caccgtttat tcggaacaag ggactgtgac atgacttttg
     4081 tcacagttcc attatttttt tagtagcttc aaagcttttt ctgtattggc aaaatgaagt
     4141 ttggcatatt tttcaagcat aggcatgccg cctttctcca atagttttgc cgctacttgg
     4201 tcagatttcg ttccagctcc tgacggaagg gtgattccag cttcagctga tgctttgtct
     4261 aactcttcca aaaagctggc acgactgatg atagaagcag cagcaacagc taaatgatat
     4321 tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt gatttttttc taaacgcgca
     4381 tattttttaa aatttgcttc cggcgtaaac tgatcgatca aaatcgcttc tggtttttct
     4441 ggctcgattt tttttagcag caaatggatc gcttgattgt gcaaagcgac tttcatccga
     4501 acggcattgt attttggttg gatctcattg tatttttctg gagataagac aagcagttta
     4561 taggggatgg ttttttttaa gatgtcagca agctggatga tttggctatc ttttaactct
     4621 tttgaatctc taacaccaag tttacgtaat tctgcaagct gatcacgttt cacataagca
     4681 gcacatactg ttagcggacc gaaatagctg ccatttccga cctcatcact acctaaaacg
     4741 gataattgac tgatattttt tggcaaagaa gaggctgttt ttgctggctt ggcttgttta
     4801 tcctgtgcct ccccccattt atcagcttct attgctgcat tcgtaccttg aaacatcact
     4861 tttccggatg tataagcggt aatggtcgtc ccgtcttttt ttgcagcaaa gctagtataa
     4921 ggaatctgtt tatttaacaa ataagagcga tagtatgctt tcatttgact aagttggtcg
     4981 tttgttgctt tgataatgac gttgctcata aaattctcct cgtttttata cataaaacta
     5041 taccgcaact cacgactttt cgttttctaa gagattgcga gacatagcat ttttttaaag
     5101 tttatcatat aatagttaag tatgtgaaaa atatttgtgg agaacggtga aagaaccaac
     5161 ttcatggtac actaaataaa actttcatca aataggagga aatgattcgt atggcgcacg
     5221 aaaaaacacg ctacaaagcg gtgattgcca accaaacata tactattatc ggacgggaaa
     5281 ccaagcatca tatggatatc gtaacgaagc tgatcaatga acagttagca gaattgaaac
     5341 aattatcacc acaaatggat aatgaacaag cagcaatctt gatggctgtc aatgcgcttt
     5401 cggatcaatt gaagaaacaa gaaagaatat tggagctgga agaagaaaca gcagaattaa
     5461 agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt gaaacgcatt gaagcaatcg
     5521 agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc agatcatacc attaaaaatc
     5581 atgtggaagc acaacagatt ttaaacgaga aacgaaaagg acaaattaaa caaaaatctt
     5641 ctaattaaga tccttttatc aaaataagaa aaatttaaaa aagttagaag agaaaggaac
     5701 gtagcatgct gagtttgctg attttatttt tattgctaat tgcgtttttt tctggagcac
     5761 gtcgcgggtt tgctttgcaa atcatattcg cgattggata tgtattgtca tttattgctg
     5821 cccagcattt ttacaaacca ttagcaagtc atttggaatt atacattcca tacccagctg
     5881 tcaccccaac atcaaagtta gcattttttg atcaggtatt tgcctttcac ctagatgaag
     5941 ctttttatgc aggaactgct tttctgatta ttttgctaat tggctggctg cttacccgat
     6001 ttgtgggggt ttttgtccat ggattgacgt atgtgccgat cttgagacag gtagactgga
     6061 ttgcaggagg catcctgtca ctaatcatgg cttacgttac tatatttttg attttgcagt
     6121 tacttgcctt tgttccatta gatgtggtcc aaaatcaatt cggcgagcat agtttcgctc
     6181 gttttatcgt tgagagaacg ccatttctta ccaacaagat gtatgagctt tgggtaacga
     6241 atattttgaa ttaaggaaaa tagtgctgga aaatatttcg ttttttgtac agagtagttt
     6301 atttaagaag gctgcgtagc gtatgtccgg cacagctctt ttttcatagt gaaagataag
     6361 gtgataaaat gaataaccga atccttgaaa cattggaatt tgaaaaagtc aaacagatgg
     6421 tcagacaatt tgtagtgact gcacaaggaa aagaagaact ggcagaattg gtaccagtga
     6481 gtgagaaaca aacgatcaca aactggcttc aagaaactga ggatggattg aaagtgcaac
     6541 gtctgcgagg cgggatccct atacctaaaa ttgaaaatat ccgtccccat atgaaacgga
     6601 ttgagatcgg agcagacttg aatggagtgg agcttgcaca agtttccaga gtattgtcta
     6661 cgacaagcga attgaatcgt ttcattgatg atctttctga cagtgaaatc gaatttgcac
     6721 gtttgtatat gtgggcagat cagttagtca cgatccctgt tttgagccgc cgtttaaagg
     6781 aagcaatcga tgaagacgga cgcgtgactg atgacgcttc tcctgagtta aagagcataa
     6841 gacagaacat tcggcgaagt gaacaagctg ttcgtgaaca gttagatggg atcgtccgtg
     6901 ggaaaaatgc aaagtattta agtgatgcga tcatcaccat gcgaaatgac cgctacgtta
     6961 tcccagtcaa acaagaatac cgcggtgtat ttggcggtgt agtccacgat caaagtgctt
     7021 caggtcagac tttatttatt gaaccaaaac aagtggtgga tttgaataat cgtttgcgtc
     7081 aataccaaat cgctgaacga aatgagattc aacgtatttt aagtgaactt tctgctgaac
     7141 ttgttcccca tcgtcaagaa atcatccaca atacttatgt gattggtaaa atggatctga
     7201 tgaatgcaaa agcacgattt ggtaaagaag taaaagccat cgtgccggga atcagtgaag
     7261 acaatcatgt cgtcttgaaa caagcaagac atccactgat cgatcaggaa aaagtagttt
     7321 ctaatgacat cacaatcgga aaagactatc aagccatcgt cattacagga cctaacacag
     7381 gtgggaaaac gatcacgttg aagactttag gtctgttaca actgatgggg caagcaggat
     7441 taccgattcc tgcaggtgaa gaaagtcaaa tcggtatttt tgaggaagta tttgcggata
     7501 taggagacga acagtcgatt gagcaaagtc tatcgacctt ttcttctcat atgacgaaca
     7561 ctgtagatat tttatcaaaa gtcaatgaaa aaagtctggt attatttgac gagctcggtg
     7621 caggaacaga tccgcaagaa ggtgctgctc tagcaatcgc aatcttagat gatcttggta
     7681 aaaagtctgc ttatgtgatg gcaacaacgc attatcctga attgaaagtt tatggatata
     7741 atcgggcaaa tacgattaat gccagcatgg agtttgatgt ggatacatta agtccaacgt
     7801 atcgtttgct gattggcgtt cctggacgaa gcaatgcgtt tgaaatctct agccgattag
     7861 gtcttgatac agaagttatt gatgaagcga agcagttgat gaatgatgag agccaagatt
     7921 taaacgaaat gatcacagat ttagagaatc gccggaaaat ggcagagacc gaatatctgg
     7981 aaatgcgtca ttttgtttct gaggcacaag aacttcacga tgatttgaaa gaagcttaca
     8041 gctatttctt tgaagaacgt gaaaaagaaa tggaaaaagc aaagaaaaaa gcaaatgaag
     8101 tcgtgtcaga ggcggaagaa aaagcagaaa aaatcattgc tgatattcgt aaaatgcaac
     8161 agcagattgg gcaaggaaat gtgaaagagc atcagttgat cgatgcaaag acccaattag
     8221 caaatctaca tcaagaagaa actttgaaga aaaataaagt attgaagaag gcgaaagaac
     8281 aaaaaacgtt gaaaccggga gacgaagtgt tggttacgac ttacggccaa agagggacgc
     8341 ttttacgtaa aaacgggaat cagtggcaag tagaaatcgg tatattaaaa atgaatgtgt
     8401 ccgaagacga gctgacgcct gttgctcctc aaaaagagcc gacacaacga gttatccatg
     8461 ctgtacgatc tgaatctagt agtcacgtgc cgaatcaatt ggaccttcga ggaaaacgat
     8521 acgaagaagc gctaagtgag gtagatcaat atttggattc tgccattcta gctggttatc
     8581 ctcaagtgac tattgtccac ggaaaaggaa caggcgcact tcgcaaggga attactgatt
     8641 atcttaaaaa ccatcggagc gtcaaaagct ttgagtttgc accggcaaac caaggcggaa
     8701 atggtgcgac aattgttaaa tttaagtaaa gcttactaaa ggtaagcaga tggttcgcta
     8761 tccaagtata gaaatgctat actagtcata gattttaaaa atggaggtct ttgatatgtc
     8821 acaagtaatt acagataaga catttaatga agaaacagat aaaggacttg tattgattga
     8881 tttttgggca acttggtgtg gtccatgtcg tatgcaagca ccgattttgg atcagttgga
     8941 acaagaatat gatgaagaag aattcagaat tgctaaaatg gatgtggatg aaaatccaga
     9001 aactcctcaa caattcggta tcatgagcat tccaacatta atgttgaaaa aagatggtca
     9061 agtagtagaa aaagctgttg gtgttcattc aaaagaacaa ctacgtcaaa tgatcgatca
     9121 atatttataa aaacttacag caaaaataag ctgagggtgg gactgaatcc agttccgtcc
     9181 tcggtttttt tgcggttgag ttaaggttat tcaaattaat attttttgag tataattacg
     9241 ttgtcaggct ggattgtttt tttgacagac atgctataat aactttaatt gaatatgaag
     9301 gggagtacca gatgaacgaa cgaatcaaaa acaaattagc tttacttcct gatcagcccg
     9361 gctgttactt gatgaaagat aaaaatggaa cgatcattta tgtcggcaaa gccaaaatat
     9421 taaaaaatcg tgtccgctca tattttactg gtagccataa tacgaaaacg gaacggcttg
     9481 ttagtgaaat cgaagacttt gaatacatcg ttacagaatc gaatatcgaa gcattgctct
     9541 tagagatcaa tctgataaag aaaaatgatc ctaaatacaa cattatgtta aaggatgata
     9601 aaacctatcc tttccttaag ataacgaatg aaaaatatcc ccgtttggtg attactcgta
     9661 aggtactaaa agacaaagca ctgtattttg gaccatatcc cgatgtcggt gcggcgaatg
     9721 agacaaagaa gatcctcgat cgattatttc ctctgagaaa atgcaaacct agtcagacga
     9781 aggagccttg tctttactat catttgggac aatgcctgtg tccttattat ttcgacgtag
     9841 atccgaaagt ctatacgaaa atcgttgatg aagtgaaaaa ctttttaaac ggaggacacg
     9901 aaaaaatcga gtcagagatc catcagaaaa tggaaaaagc tgctgaaaac atggagtttg
     9961 aaaaagctgc agaataccga gaccagatcc gtgccattga gacaatcatg acttgccaaa
    10021 aaatgacaaa tactgattta ctggatcgag atgtatttgg ttatgcgatt gataaaggat
    10081 ggatgtgtgt ccaagtgttt ttcgtaagac aaggaaagct aattgaacga gatgtttcaa
    10141 tgtttccttt ctataacgaa gcagaagaag atttcttgac ttacctcggt cagttttatc
    10201 aggaaaatga gcatttcatt cctaaagaag tcttgattcc tgatacgatc gacaagccca
    10261 gtgtagaagc tttgttagct acgaaagtgc tgcaaccaaa acgaggagaa aagaaaaaat
    10321 tagttgaatt ggcgagtaag aatgcctcag ttgctttgaa tgaacggttt gatctgattg
    10381 ccagaaaaca ggaacggacg atcggagcag ttgaacgttt aggaaacgcg atgaatatcc
    10441 cgaccccaat acgtatcgag tcttttgata actctaatat catggggacg aatcctgtgg
    10501 ctgcgatggt cgtattcatt gatggaaaac cggcaagaaa agaatatcgg aaatataaaa
    10561 taaaaacagt caacggaccg gatgattatg catcaatgag agaagtcatc tatcgcagat
    10621 attcgcgagt gattcgcgaa gggttgcctt tacctgattt gatcttgatc gatggaggaa
    10681 aaggacaagt agatgtggca aaagatgtct tagaaaatca attaggaatc gatattccta
    10741 ttgctggcat ggtaaaaaac gataaacata aaacaaatga gttgctattc ggtacagatt
    10801 tggctgtagt accgcttgag cgaaattcac cagagttttt cttactccag cgaatccaag
    10861 atgaagtcca tcgattcgca atcactttcc atcggcagct acgcagtaaa aacagttttg
    10921 cttcccgatt agatgaaatt gaagggttag gaccaaaacg aaaaaaaatg ctgcttaaag
    10981 agttcaaatc gttgaaaaat atcacagcag ccacgacaga agaattacaa gaaatcggac
    11041 tgccgaaaaa tgtcgcacaa aacgtatttg ataaattaca tcaggaatga ggaaagggca
    11101 ctttacaaag aaagtaaagt gtcctatcat tttatgtaca acaaaaagag cgtttacgtt
    11161 ttgttggtaa ataataaatc gcttacgtct ggtgtctaag gatatatgtg atttcttgaa
    11221 gaaggagtga agaaatgtta gaagtcaagg atcttgttaa aacttttggg aactacacag
    11281 ctgtcgatca tgtctctttc caaatccccg atggaaaaat catggggcta attggccaaa
    11341 acggggctgg gaagacaacg acttttcgtt tgatcttgga ttttttgaca caagaccaag
    11401 gagaagtgtt atggaatggc catcatcttg gggagaaaga ttatgatgtc atcggttatc
    11461 ttccggagga acggggactt tatccgaaag taacgattca agaccaactg ctttattttg
    11521 cagaacttag agggaaatca agaaaagaga ttgaaccaaa aatcgacttt tggatggaaa
    11581 aatttcaggt aaaagggaaa aagacggata aagtaaaatc tttgtctaaa ggaaatcagc
    11641 aaaaagtcca gctgatcgcc acgttaatcc atgaacctaa attgatcatt ttggatgagc
    11701 cattcagcgg gttagatccg gtaaatgctg aattgctaaa agatggaata ttggaattga
    11761 aaaaacatgg ttcatgtgtc attttttcta gccacaatat ggataatgta gaaaaaatct
    11821 gcgatcattt gatcatgttg cgaaacggaa aaatggtttt gaatggaaaa gtacatgaga
    11881 ttcgtgaatc atttggccgt acgaagttat tcttggaatc tggtttaagc caacaggaag
    11941 tagaagaaat cgaaggcgtg aaaaaagtga tccttcatga agacaatacg ttagagatta
    12001 ccttagaaga tcctgaagct gggaaagaaa tctttactcg tgcgactcag ttcggctaca
    12061 ttcctatgtt taaccagcaa ccgccaactt tggaagagat tttcaaattg aaagcaggtg
    12121 aaatcgatgc gtaagttttg gattattacc aaagatgtct atttaaaaaa tgttaagtcg
    12181 atttctttct tgatcatgat attagtccct tttatcatgc tgggtatcat ttatgtagca
    12241 ggaaactttg ctcaacaaaa tagtgatgtc aataaaatcg gcgtattggc tgataatgag
    12301 caaatcagtc agcaaatgag ccaagtcaaa acagatgatt ttgagttcaa agcagttgat
    12361 tctgaagaaa aagcacaaaa agagctttcg gaagaaaaaa tcgatgcgta tttagtagtc
    12421 tcaactgaga atgataagat aaatgggatg ctttatagcg aaaattcatt aggacaaagt
    12481 acacaactac tgatacaaca acaactgact ggcttgcaat cgatgcttcg agcgagtcaa
    12541 ttaggcattt caccagaaga agtagctagc ttgagccaac aagcaagttt gactcgacaa
    12601 aaagtcaatt ttgattcaaa aggaaaaatg acagtcggag aagataactc gacagtccaa
    12661 tacgtcgtaa gttatgtagc aacgattgtc ttgttcatta ttattttgac gtatgcgcag
    12721 atcattgcac aagagatcgc ttctgaaaaa ggcacgcgga tcatggaagt gatcctgtcc
    12781 agtacgactg cacaaaagca tttttacgga aaattaacag gtgttttact tgttgcatta
    12841 acgcaaatgg cattatacgg tattattttt ggtataggat acagtcaatt caaagatatg
    12901 gaaatggtca aaaatatatt agacggtatc tcattggata acttgattgg accattcttg
    12961 tggtattctc ttctattcat gatctttggc attttgattt tttctgtttt agcagcttta
    13021 tgtggctctt tggtcaacaa agcagaagat acagcaaaag caattttgcc agtgacttat
    13081 ctatctctag gcggatacat gcttggattg atactaggag cttcagatcc aaacaatatc
    13141 gttattcgga tcacttcgta tattccattc ttatcttcat atattatgcc gattcgctta
    13201 gcaaatgaga cagtcggtat gggtggagca ttgatttcct tgttggtact tgtagccgta
    13261 acattcttat tgatgatctt gtctgcaaac atgtacaaat cgaatgtact cgtttatagc
    13321 gaaggcggat tatggagttc attgaaacaa tcgatttcaa ttatgaggaa tgaacgaaaa
    13381 aaagcataaa acaatcctaa aatacagaag gaagctggac atctattgtt cccagcttct
    13441 ttttgtatag acctatttta tttattttac tatattggat atatattcat tgaaaatagt
    13501 gtataacaac tttataatat tgagtatacc aatttttgga ggcgttttaa tggaccgtta
    13561 tttagattta ctttcagaaa aattcccctc aacggaggat gtcatcacgg aaatcatcaa
    13621 tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagtg atatacatgg
    13681 cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat
    13741 caaagacctt ttttctgaac aaatgacgga aacggatatc agccaattca ctatttttac
    13801 ggcttatcca gaatatgcat taacgactga ttggtacaaa gaacaagata aatcgcagct
    13861 gatccatcat ttgatcgatc ttttacggtt tacgacagtc aaatataccc ggtcaaaagt
    13921 tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga
    13981 ccgtatcaat ggaaaaaaag actatgtaaa aaaaatcatt gaacaacttg ttttgatgaa
    14041 ggaagaagaa aaatttttaa cgaaacttgc acagacgatc caaaaatctg tcatcgatca
    14101 tttgcatatc gttggagata tttttgaccg aggaactcaa gcagccaaag tgatggatcg
    14161 aatcatggat ttttcttctg tagatatcca atggggaaat catgatattt tgtggattgg
    14221 tgcgtattgc ggctctgagg catgtttact gacactttta cggattgcag cacgctataa
    14281 ttatttattt gaattagaga aagaatatgg gttgaattta cgtccattgt tttcttttgc
    14341 tcatcaaact tatcagaaga atccagtatt tgcgcctaaa aacagtaaaa atctatccga
    14401 aagagagcaa gaagaattag aaaaaatcca tcaggcactt tctatccttc aattcaaatc
    14461 agaacatcag ttattggatc gtcgacctga atttaaaatg gacgatcgta aattgctgga
    14521 aaaaatcgac tatgaagcat caacgattga tttagaaggt cagctctatg agttgaaagg
    14581 aacttgtttt caaacgatcc agccagatga tccaaaaaaa ttcttggaag aggaacaaaa
    14641 agtgattgat agtttgatgt attcattcca gcactctttg agaatgcaaa aacatatgac
    14701 gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtgc tgtttcatgg
    14761 ctgtatacct gtagacgaga agggacagct cttagcattt gaattagacc aatcttatcg
    14821 cggaaaagaa ctgctgggct tttttgaaaa acagataact gaaagcgcaa aagatctgac
    14881 tgcaaaagaa gatttagcaa ccgatttgat ttggtatgcg tggagtgggc cattttcgcc
    14941 tttattcggc aagagtaaaa tggcgacttt tgaacgttat tttctcacag agcctcatac
    15001 gcacgtcgag aaaagcaatc cttattatca cttgcgagat gaagagtggt tcagtgaaaa
    15061 aattttagca gaattcgaca caaaagaaga agggtcagca atcattaatg ggcacacgcc
    15121 agtaaaagta aaaaaagggg agtcaccgat caaagcaaat ggaaaagtat ttgtcattga
    15181 cggaggttta tccaaagctt atcaaaaaac gacaggaatt gccggttatt cactattatg
    15241 caattcttat ggtttccaaa tcgtgacaca ttatccgttt ttgcctattg aacaacaatt
    15301 tgcaaaaaaa tccgaccaaa caaatgtgaa aaaagtaatc gaacagcaac taccacgcaa
    15361 actcaatcgt gatacaacaa aaggtatgga actgcagcag cagattaccc agctcaaaag
    15421 gctgttggac tatcgaaagg acgattgaga agaattccag tgaacattgt tccatagact
    15481 ggagactgag acaaaagtcg tgtcgcagtc tctttttccg gataaacggt gttcttctgc
    15541 caactcctcg gtcacaagtc acactattca acaaacatga aaagctgttt tttgaaattg
    15601 ctccttgtga ctcagagttg aacggcaggc tcacgacctc ttggtataaa cggtgttcaa
    15661 aagccagttt ctcggtatta agccaaattt atgcaaaaat gtgaaaagcc attttcacaa
    15721 aatttttctt aatactcgaa actaaacagc tttttcacaa ccccctcaga aagtctatta
    15781 aaattcattc acaaagaaat cgaataagcg ctgttcagat ggatgtacac ggtgggtaag
    15841 ttccggatgc cattgaacac cgaggatttt atgcatatca tctttgcttt gaaccgcttc
    15901 aacgagtcca tcgttagaaa aagcgatggc ttttaataaa ggtgaaagat ctttgactgc
    15961 ctgatgatgg taagaattca caaaatatgg atcggggaga aggttgctta acaaactgtc
    16021 tgaaacaatc ttgacttcgt gagtcgtaaa ttgcggggca gtgggctgtt gttcatgttt
    16081 tactgaccac tttggatata atgaaagatc ttgataaagt gtaccttcca aaacgacatt
    16141 caatagttgg agtccccggc agactgcaaa aattggtttt ctttgtttca atgcttccaa
    16201 gatcagtgcc tgttcaaatt ggtctcgttg aatatttgtt tccgttagct ttggatgagg
    16261 atcttccatg taaagttgtg gactgacatc ttgaccacct gcaagaataa gtttgtcgat
    16321 ttgtccaatc agttgaggag ctgaatctgg agacatgact ggcagaataa gaggcaatcc
    16381 ttgagcatct aaaactgcat ccacgaatcc ttgcggtgta tagctcactt gatttccttg
    16441 gaaagtatct gttgctcgaa tcagttgatt tccggcaatg ccgatgattg gtttcatttg
    16501 tattccccct aaagtagact tattgtaaca aggatttaaa aaaaattaaa ctatgtaaga
    16561 cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa atttcgtgtg
    16621 aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa
    16681 taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt
    16741 attgaagggg cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag
    16801 aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa
    16861 caaatccgaa aagcagaaga agaaaatgaa aagcgtgctc aaacactgcg gcagcagctg
    16921 aagatctatg caacagaaaa agagcaggaa ttggaaacga tccaaacaaa ctatcgacaa
    16981 caactaaaac agttggtgga agaaaaagaa caatttgaaa tacaagaatt agaaaaagaa
    17041 aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga tcaatatcaa
    17101 aagcatcaga gtcaagcgat cggtgcgatc attgaaaggg tgaaagaaac ttatggccgt
    17161 cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca
    17221 agcagtacaa ggaatccaaa acgttgaaat ccgggatctg tatcaaaata gaacaaacaa
    17281 ccagtgggtg gagaagtatt ttccagatac cacgattatt gataaagaag caaaattaag
    17341 cgctttaggt aatcggttga aagacatcca agaagctatc caattcatac aacaccacgg
    17401 cgatagccgt caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagatttgga
    17461 agccaattat tccgaagaaa atttcataaa taaattgaaa gaagtccttg atttaaaaga
    17521 aaagtgggaa caactaactg aagaacgtga tcgtttagaa gaagaggaag aatggcttct
    17581 tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga
    17641 gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactga gtaagcttga
    17701 tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc
    17761 tttaaaaaaa tcagttgctg ttatagaaga aattgctggg aaatacgggt tcaataaaga
    17821 aacgtatcct tatgaacaaa atcctaaaga aaaactgcaa gagaccaagc agcaattatc
    17881 cattgttcac gagcagcaaa agaagataac gacagcgtta ggtcagtgca gtggctatat
    17941 ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacgag aaaaaataaa
    18001 agaacggttt atccatgcga cttatctgat cgttttgcaa ggttgggtag atgcagaaga
    18061 aaaacagaat ttgctgcatg tgttaaacga aaccgtatca gaagaagata tatacgtcag
    18121 tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca
    18181 tccgcttgtc gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga
    18241 agtagatcca acaccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc
    18301 agatgttggt tatggtttgc tgatgctcat tgggacaata ctagctcaaa aactacttgt
    18361 tctaccacgc gggatgaaac gtttcgtaaa attctttgag attctctcga taccatcgat
    18421 catctgggga ttgatctaca gttcattttt cggacagtca ttaccaaaag aactgtttgg
    18481 catccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt
    18541 atccgtgatt ttcggtctga tacagatttt ggttggatta tttgtggcag caaaggaaaa
    18601 tatcaggaga aaagattact tgagtgctgt cagtgatgga tttgcctggc aagggatcct
    18661 aattgggatc gttattgcgt tggttggtgc catgttgata aaaaatacag catttgttta
    18721 ccttggcgct gggctatcga tcattgctgc cttatgtatc ttgatcgttc cgattatcca
    18781 aacgccgtct aaagtcaaag gtgctgccaa aggtgcgtat aatctttacg gactgaccgg
    18841 ctatatcggt gatttagtca gttatacacg tttgatggca ttgggtattt caggcggaag
    18901 tattggagcg gcattcaata tgctagttgc tttcatgccg ccagctgctc gattcagtgt
    18961 agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac tgctaagtgc
    19021 ctatgtccat ggcgcacgat tgcagtatgt cgaatttttt gggaagtttt atacaggagg
    19081 cggacgtgcc tttgatccgc tgaaaacggc ggaaaaatac gtcaacatca atcacaagaa
    19141 aaaaaacaat aaataattgg aggattttta tataatgatg gattacttaa ttagtcaaaa
    19201 tggtggaatg gtatttgcgg tattagcgat ggcaacagcg acgatttttt cagggatcgg
    19261 ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcattgacaa caagtcaacc
    19321 cgaaaaattc gggcaggctc tgattctgca attgcttcca ggcacgcaag gtttgtatgg
    19381 attcgttatt gcctttttga tttttatcaa cttaggaaat gacatgtcag ttgtacaagg
    19441 actgaacttt ctaggtgctt ctctgccgat tgcttttaca ggcttgtttt caggtattgc
    19501 ccaaggaaaa gtcgcagcgg caggtattca aattttagcg aaaaaacctg aacatgctac
    19561 aaaaggaatc atttttgctg cgatggttga aacatacgcg attttaggtt tcgttatttc
    19621 cttcctatta gtgttgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa
    19681 aaaatcatct ctcagatgaa cgaagcagca gagcaggaaa gagcggcgtt agaacaagaa
    19741 gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaagttga aacagaacat
    19801 caaaaacaaa aagaaaaaca aatcgaatta ctcgaaaaaa aataccgtca gctaaggaat
    19861 cgccagcaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttaagacga
    19921 ttgtttgcag atgcggtaac tgaaatggaa aattgggatg aatctgaaca aatccagttt
    19981 atcaaaaatg cattgtattc attacctttg acaggaaaag tagcattcat tgctggagaa
    20041 aaatcagcag cctatctttc tcagacgttg ttggatgaat ggaacaatga acttcccttt
    20101 atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa
    20161 ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt
    20221 tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac
    20281 taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga
    20341 tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc
    20401 catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc
    20461 gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga
    20521 tgcgttatac tttccataat ctgaaggttt tgacaaaagc tgagatgaca aatcaaaatc
    20581 tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc
    20641 atacgcaagt ttcaaatgaa cttcctgaaa gcattatgga cagtatacga gaagtacgtg
    20701 attattttga agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt
    20761 tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca
    20821 ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa
    20881 catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt
    20941 tgaactttgt agataaagat atggaagatt acacgaattt tctattagag acagattatg
    21001 ctgattttct gcgccctgtc atccgtgagg gaaatattga tctcgtaaag cttgaacagt
    21061 tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac
    21121 tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg
    21181 ttatcggcaa aagaagccat ttcgaatcag aaacaatcag aaagcgggtg agacaagtct
    21241 atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt
    21301 ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg
    21361 gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa
    21421 acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa
    21481 ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa
    21541 aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc
    21601 tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta
    21661 gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt
    21721 caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa
    21781 gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg
    21841 ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg
    21901 cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg
    21961 gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc
    22021 atggtgccaa atgggataaa aggaacgatc aaaacaatcg aaaaaggttc attcactatt
    22081 gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa
    22141 aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg
    22201 atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct
    22261 gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc
    22321 gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt
    22381 ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg
    22441 gtattgattg ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg
    22501 ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac
    22561 tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct
    22621 ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct
    22681 ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct
    22741 gtatccccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta
    22801 aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat
    22861 tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt
    22921 caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag
    22981 ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca
    23041 ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc
    23101 gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt
    23161 gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc
    23221 gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa
    23281 ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc
    23341 atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt
    23401 tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga
    23461 gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga
    23521 aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga
    23581 actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga
    23641 taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc
    23701 agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt
    23761 gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa
    23821 agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc
    23881 cgtagtcttt gctgctattg gaatcacttt tgaagaagct gaatttttca tggaagattt
    23941 tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc
    24001 aatcgaacgt atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga
    24061 aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg
    24121 agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta
    24181 cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt
    24241 tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt
    24301 gactggttat attacagaag gacagatcat cttgactaga gagctttaca agagtgggat
    24361 tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc
    24421 cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca
    24481 aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa tctgctttat ctgatgtaga
    24541 caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta
    24601 cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc
    24661 aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa
    24721 gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa
    24781 aaaacagttg acaactgcca caagagggca taaattatta aaagataagc aagatgaact
    24841 gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga
    24901 gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggaaccttt
    24961 catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa
    25021 tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac
    25081 acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt
    25141 tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat
    25201 ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc
    25261 tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga
    25321 agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatctttttt
    25381 taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgttt gccggctgtt
    25441 cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta
    25501 ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact
    25561 tctgctgtct gtgtaacggg attgacaaca ttgaatacag cagagcattg gaacagtgct
    25621 ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg
    25681 attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa
    25741 gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctaaaa
    25801 ttggcctttg tgatccaatt gattggtacg gttgctttat cttttgtatt tgtgcctgaa
    25861 tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat
    25921 gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc
    25981 atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta
    26041 ctggaatatc atcagaaaaa gaagatgacg attcattcaa aaatcgcatt aaccgttaca
    26101 ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggcaacg
    26161 ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct
    26221 agaacggctg gatattattc agttgattac ctccatatga gccatgcagg tctgatttta
    26281 acgatgtttt tgatgtacat cggcggaaca tctgggtcga cggccggcgg attgaaaaca
    26341 actacgttag gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg tacgagagcg
    26401 gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt
    26461 gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct
    26521 aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt
    26581 cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt
    26641 atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa
    26701 agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt
    26761 ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg
    26821 gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg
    26881 ggtagtgcct cttttttatt gaaaggaatc aataagattt gattccttta tttatcaata
    26941 ggttataatt catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt
    27001 tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta
    27061 aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta
    27121 gggtcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt
    27181 tctactttga aaagagaatc aaaaagctga ttggctcttt ttgtaagact taatttagtt
    27241 tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat
    27301 ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgctttg gacatgtact
    27361 ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag
    27421 tagattgttt ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa
    27481 gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact
    27541 tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa
    27601 ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt
    27661 cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat
    27721 tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt
    27781 gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag agattctaag
    27841 gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg
    27901 cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga
    27961 ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg
    28021 atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata
    28081 aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc
    28141 ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac
    28201 tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat
    28261 cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca
    28321 taagaattaa taggcatttt ctccactcct taaaattgta taccatacaa ttttaccatt
    28381 gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt
    28441 aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa
    28501 tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta
    28561 cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga
    28621 tattttggca caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct
    28681 catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac
    28741 gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta
    28801 gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat
    28861 ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa
    28921 gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata
    28981 aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct
    29041 agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg
    29101 ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc
    29161 tatgtagatg attactcagc tgccttaaca gaaagcgggg acctgcttat agccatggca
    29221 gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa
    29281 aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt
    29341 gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga
    29401 acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag
    29461 cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa
    29521 acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt
    29581 ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt
    29641 tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc
    29701 tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa
    29761 aagtgccgat attgtctgaa tatgaagaag cgattggggc taagattaaa gaagcctact
    29821 tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag
    29881 attttacagc tatcaaaaat tttgaattag gaataggagt catcgtgaca gcagaaagtg
    29941 tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt aacaatcaat gaagatccag
    30001 tatgtggatc agcacattct aatcttattc cttattgggc agaaagaatg aataaaacaa
    30061 aattgtcagc ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag
    30121 atcgtgtgat aataggagga aaagcagttt tgttttctaa aggtacctcg tttatttgat
    30181 ttctttcaaa tgaattaaaa ataaattggt ggagagtggc aaccttatag ctacaatggt
    30241 tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag tttgcgtctc attttaatta
    30301 ttggtgtttg ttttttattc aatcaagagt aaaatgcccc atactaacta tcctcggatg
    30361 acttcgattt tataaccatc agggtcaatg acaaagtagt aagaaggcgc agtgccaggt
    30421 aatcctttta agtcagtaat ctcaagacca gcagctttat gttgctcgtg taatttttct
    30481 agatcatcag ctgctatagc gatatgacca tagccgtctc ctagatcata cgcaccatga
    30541 tcatagttgt aagtcaactc taattcatag tcatccccag gaagtgtcaa ataaacaagt
    30601 gtaaatttgt tttctggaaa atcacggcgg cggctttctt caaaaccaaa tgccttttga
    30661 taaaattcga ttgatgcttc aagatctttc actcgaacac atgtgtgagc cattttcata
    30721 gattcatctc tccttttcct tttactcaat cataacaaaa agaagtaagg aaatagttga
    30781 tttgtgcttg ttcatgagaa atttctcatg taaaatggat agaaaataaa actgttaagg
    30841 tggaaactat tcatggataa accaattttt ggtgaaaaaa agcaatttgc tgattatcaa
    30901 acccgttatg cagcatatat tgtcatagaa agagatcagc agattgcagt aatcgaggct
    30961 ccaaatggtg ctttttttct tcctggcggg gaaattgaag gagaagagac gaaagaagaa
    31021 gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg cagaaatttc ttcttatctg
    31081 ggagaagctg atgagtattt ttactcgaat tatcgtgcaa cttattatta taatccagga
    31141 tatttctttg ttgctgattc ttggaagcgt ataggagaac caactgaaaa gaccaataaa
    31201 atttggtggg tgagccctca agaagcgctt gtgaaattga aacgtggtag ccaccgatgg
    31261 gcagtcgaga gatggatcga aaaaaataaa aactgaaaga aagcgagtag atcagctttc
    31321 tctcagtttt tttatttatt acagtacata ttttttgatt gcttcagcta caccgtgctc
    31381 gtcgtttgat gaagtgatga catttgctgc ttctttgaca agagaaacgg catttcccat
    31441 tgctactcct agaccagcat attcaatcat ggataagtcg ttctcgttat ctccgatagc
    31501 catgatttca tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt
    31561 acttgcttct ttatttagta cttcgaagta atagggcgca cttttgaccg ttgtgtattt
    31621 atcacggaaa gtttgcggta aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat
    31681 catcatcatt ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc gatattttag
    31741 cggcatcttg actaaacttg cttcgtggat cgtataagcg ctgatatctc gattaggcgt
    31801 gtagatcgct ttgtcatcga tcgaatggag atgactgccg acacggcgtg ccattacttc
    31861 gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt
    31921 ttgttgtacg agtgccccgt tataagtaat aacgtaatca ttatcttgaa aaaggttcaa
    31981 ctcgtttaac tgttcctgga cacctggtaa gggacggcca gtgcataaaa cgatcgaaac
    32041 accttttgca acagcagcgg caatcgcttc ttttacttct tttgttactt ctcttttctc
    32101 atttaataaa gtaccatcga tgtcgatagc gactagtttg atagacataa tttcctccaa
    32161 tatattttaa tttatttcaa tcaatgcgcc attacggata tggctggcaa attcttgata
    32221 agtttcgtca aataaatcat aatgatcacg caaggacgga tcaaccattt cttttggaaa
    32281 gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca gcaacaagct gactaacctg
    32341 tgataattct atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc
    32401 ttgttctggg cgataaaaat cataaggtag atcgtagcta gaattcacag cagtgtaata
    32461 aacaggatta tatccgacct tttctactaa caaagtgagt tcttggacta attttgaatc
    32521 ccggttgcca gaaaaagtag cagatttcag tggtttacga ttcaaaaaac gtttagctaa
    32581 atcatttaaa ataggatcag cagattcaga ccattgagta aagtaagtgc ccaagacccc
    32641 atcatcaagt ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa
    32701 ttgagaatgg agttcaaaac tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag
    32761 cagatgctcc aaaattactt ccataccacg ggaaacagga tggaagtaca cttgcacata
    32821 catttgatat cggctaacga tataatcttc cactgcatgc atgccactca tggaaaaagc
    32881 gatcccgcct tcataaggac gaatcacacg taatattcgg gtaagatcaa atgttccata
    32941 ttctgttcca gtgaaataag catcccgaag caaatagtcc attcgatctg catcgatctg
    33001 actagaaatc atttggacaa cttgtgggtt ggggtaagtt ttttggatca cactagctac
    33061 cttttcagga aaaccttctt ctactcggtt aagaatctga tacacttctg tttctggaga
    33121 agtgatgatc tctacagtga tggcttcatg attcgtatgg aagatatgtt caaatgtatg
    33181 agagtaagga ccatgaccga catcatgcaa cagtgccgca cataaagtca ccagccgttc
    33241 ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa aaatttcgag aaaagatatc
    33301 gcagattcgt cttgaaatct cataaacgcc taatgaatga gtaaaacggc tatgttctgc
    33361 tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc
    33421 tttcgaattg atcaaatcta agattacttg atgctggaca tggatatagt tatggactgg
    33481 atcacgaaaa actttttcca taggcagcat ttgatattta taaggggttg tcataatgta
    33541 gctcctttat gttattacgt tgttccattc tctggatttg gttgttccat tgctccagct
    33601 ggatttcttt ttttagccag tcggtagaat cctgtaaatc acttgcagaa tacagctttt
    33661 caaaagcttt tgcaaatgct tctttgactt cttttacaaa aaggggttgg tcaagcagtt
    33721 cttcaagtgt tgccattgta gcaggatcca ctgcaggata gccatttgta ccaaagtctt
    33781 cttttaaagc agattgataa aatcgcttca tgacttgccc acgtttttcc tgattaccgt
    33841 tgacacttaa gtacatcata acagaaatcc catttttgat ccgacgctga gcgataccgg
    33901 caaatttttt gccatggatg cttaaatcat aagttccagg acaataagaa tgggtgattt
    33961 cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt caataggagc atttgctcgt
    34021 aagcgtcatc tatggttatt ttttttgtgt cactttgggg gaaaatcaaa gaaatattca
    34081 aaattccttc atctgatacg atccctaatc cacctgaatt tctgatcact ggagcgtaac
    34141 ctgcatcttc cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca tccttcatac
    34201 ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg tgttctttag
    34261 aatattccgt taatatatct gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt
    34321 gctgatctag tcgaatcgca gggttcattc aaccggctcc tttgtataaa ttcatatttt
    34381 ctacaatatt atactctaaa atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat
    34441 ggacaagttg gttgacaaaa caaaagaaac taggaacaat gtagctatga gaagtaaagg
    34501 agaatgaata atggaattat cacaaggaac accgatttca atcaaattgc gtacaaaagt
    34561 aaaacagcaa ggggaagtgc aagattttta ttttgatttg aaagggcaaa tggtgacaat
    34621 tggcgatacg ctgtacatac gttataaaga gattcaggag gagacgggcg atgagattcc
    34681 tgtaacgatc aaattagttc cagatggtca tgtacagctg atccgtgcag gagaaatgcg
    34741 gatgcgtttg aaatttggct ataaagaacg gttagaaaca tcctatcgaa caccatacgg
    34801 tatgatccag attgctactt ttacgaaaga actgcatgtt agtttgaaag accgtcccac
    34861 agcagggaag gttcgaatcg attatgatct gttcatggga ccggaaaaaa tcggcgaata
    34921 ttatttgacc ttggatttta cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg
    34981 attttgtatg ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt
    35041 tgaaggtgca aacaaaagcg aattatcaat gatcgaagta gcacacgcga tcttagaaca
    35101 acgtggcgat gtcatggatt tttctgattt agctaaccag atccaaaact atcttgggaa
    35161 atcagacagt gaaattcgtg attcactcgc acaattttat actgacttga atattgacgg
    35221 cagctttatc tcattagggg ataatcgttg gggcttgcgt tcatggtatg ctatcgattc
    35281 gatcgatgaa gaagtaaacc atggaatgga cgaagaggat gaagatactc cacgtcgccg
    35341 taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg attacaatga
    35401 cgatgaccca gaagatacag atctgacaga agaagatgac gatgatctgt ttgatgacga
    35461 tgatgatgaa gatgaagaaa tcgctgctta taattctgac ttgcaagaaa tcggagcaga
    35521 tgacccgaca gatgatgaag aagacttgcc aggaaatatc gaagaagatt taagtatcat
    35581 agacgaagac gatgatgatg aagattatga agaggaagaa ttttctgact ctgaaaaaga
    35641 ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc agaataagga
    35701 ggtgtgacac gatttttgtc acaccttctt ttttgtatat aaaaatccca agtaaataca
    35761 taaaaaataa aaaaattgaa acttttagcg caccttgtac gtcatattaa gtgaggcaga
    35821 aaggagtgga gaatgatttt tactggatat aagaaaaaac ggcagatcga aaaataccgt
    35881 cacctgctga atggactcat cgaaactgat tatcaaaaaa tgttccgtat cacgatgaat
    35941 tacgtacatg ataaagaaga ggccttagat gtgatgcaag atagttttca taaagccctg
    36001 aactattttg aaaaagaaaa agagatcgag cattttagtg catggtttta tcggatattg
    36061 atccgcacgg cattagatag ttggcgcaga caaaaaagga cgcctgtgga cttatttgaa
    36121 attcaagata tcccccaaca gttaccttta gaaacttccg ttgcagagct ccatatgatt
    36181 ttagataaaa tagacagtcc ggaaaaagag attctgatcc tctacttttt tgaaggattt
    36241 cgtttgaaag aaattgcgac tatcttggat atgaatgaaa acacagttaa aacgaaaatg
    36301 taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa ggagagaaga gtgccatgta
    36361 ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat aaagaacaag caattcccaa
    36421 agggcttaaa gaggagatcc gccagcgttt tattatcgaa gagcaatcat ttataaaagc
    36481 aaagcggaga aagagacgtc tccaagggtt cggagtatct ttggctatcg taatgattac
    36541 aacaagtagc cttcttttta atagtcaagt tcgaagcttt gcagaagatt tacccatctt
    36601 aggatcagtc attgaattga tcttaggcga acgctttact gatcgatctg aaaaaatcga
    36661 tattcaggta ccgaagatca atacacaaaa tgagaaagaa aataaaacaa ttcatggatt
    36721 aaatcaaaaa tactttcgcg aaggacaagc agattttgaa aaagctgaaa aagaatatgg
    36781 aaattttgaa acggatcatt accaagtttt gggagattat cagaaaatcg tagacgataa
    36841 ccggtttctc gtgattgaaa ggcagatcac ccaaacagca gcagatagcc atgttgaaaa
    36901 aagatacgac acaatcgaca agaaaaacag tgtccagctc tcacttccac ttttattcaa
    36961 agatgacacc tatctatctg ttttgacaaa agaagtcaaa cggcagatgg ccgaacaagt
    37021 caaggaagat ccatctaagt actattggac tgagcaagat attcaagaag ggacgataga
    37081 aaaaccaaca cttgttacgc ctacacgaag tttttatttg aacaaagagc atcaattagt
    37141 tttgactttt tctcaatacg aaattgctcc tggttatatg ggtacaccag aatttgtcat
    37201 tcctaagtcc gtgacaaaaa cgattttggc atcggaagat tatttggatc attagacgag
    37261 aagtagttgc gactagcagt ttcaattttc aagtaagtac aagtaaataa taagaagaga
    37321 aaaagagaaa gaagagatat agtgaaaaga aattatttag ttatcagtat tattgcttta
    37381 tccgtgcttt tgtttttcga aattacttcg gtggcctcct tgatcaccga agtaaaagga
    37441 caaacacact cttcggagca ttcgtcacaa tctttcaaac aatcagaaca atcaaccatt
    37501 aaaagccaat caactgaaac agctaacgtc aaagaagcca ttcaagaatc agcggaaaag
    37561 gtacaaagtc agtcatcaca atcaacacaa gacagtacaa aaacgagccg agatccggct
    37621 gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag cgaccacaac accgcagctt
    37681 ctgcgaatct taaaagaaaa aaacgtgcat gccacttttt tcgtcttagg agaaaatacg
    37741 gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag ggcacgagat tggcaatcac
    37801 acatacgatc atcagaatct ggccactctt tctgcccagt cgatgacgga ggaagtgacg
    37861 aaagcagaca ctgagatcaa aaaagtcact ggcaaaaccc ccacttttgt tcgtccgccc
    37921 tatggttctg tcacgaatgt aggagcaacg atcatccagc gaccgatcat cgaatggtct
    37981 gttgattcag aagattggaa gacaagaaat cctgatttga ttttgcagaa gatccaagca
    38041 acagtatacg atggggcaat tattttattc catgatatct atcctgaaac gattcgtgcc
    38101 gttcctcaag tgatcgatta tctgaaagag caagggtatc gcatcacgac agttggggat
    38161 ttattagggc atccaacggc tgtcgagaac tattatggca gaaacgatca tcggccagtt
    38221 cagtaagtga gacaagtatc tttaataaat aacacatttt aataagtagt tgaacacata
    38281 actaccttac ttgcaaaggc ttaacaatat ctatacaggt acaacaaaaa actgtccacc
    38341 aattttcttt ggcggacagt tttcacttaa aaataaagaa gcaactattc gtaaattatg
    38401 ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc ccgtctcaag aaccggaatc
    38461 ttacgtgata tccactacac taaggacgca agcactggga taattgtaac taaacagata
    38521 aaaaattaca aggggattgg acaaaaaaat gaaatttgag caaaattttt cacaaaaaca
    38581 gcagcagaca cagaaactag ccatgacgca acagttgcaa cagtccatcc aagttttaca
    38641 atataacagt gaggagctgt tggcattcgt tgagaatcaa gcaatggaaa atccattggt
    38701 ggaagttgtt gagccggaat ggcagccaga ttacataaaa gcttcttcat cttcttatga
    38761 aggagaagag acaaattacc taaatcagat tcctgataca aaagggtctt tatttgattc
    38821 attgatcgaa caagtccatt tgaattaccg agatactttt ttgaggaaaa tcgttcttta
    38881 tcttgtagaa tacatcgatt tgaacggatt tttgacgatt agtttagaag aagccggaaa
    38941 agagattgga gctacgccta tccaaatgct cgatgcgttg actctgattc agcaattaga
    39001 accagctgga gtaggtgcac gtagtttgca agaatgtctc atgcttcaaa cagaacgaga
    39061 tgattatgcg ccggaattag cttatatcgt gttggaagaa tgttttgagg aactggtgga
    39121 gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat ctacatgctg ttcagcagat
    39181 attcgactat ttacaaacgt tgagtccaag ccctggccgg attttcgacc gtagcagcga
    39241 attgtacatt attcctgatg tccgggtatt agtggacgac gataaaaatg tacaagttat
    39301 ctcaaaccga aaaaatcagc caaatatacg ctttcaagag agttacttca aacaaatgtc
    39361 acaacaagca gataaagaaa ctgaaaatta tttaaaagaa cggaaacagg aattcgaatg
    39421 gttgaagaaa acgatcctac aacgtgggga tacaattttg cgggttgctc aagtcattgt
    39481 ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca atcaagcctc ttacgttaaa
    39541 ggaagttgca actgagatag atgtgcatga atccacggtc agtcgtgcag tcaacgggaa
    39601 atatttggaa acagacttcg gtgtatttga attaaaaaaa tttttcacta cacgaattcc
    39661 tacgaatagt acagaacaga cagaagacct ttcagcagat acagcgaaga aaaagctgca
    39721 ggaattagtc gatcaagaag acaaaaacaa gccgttgtct gaccagaaat tggttgaatt
    39781 gctgaaaaaa gatgagatag cgatctcaag acgaacagta gcaaaatatc gtgatttatt
    39841 aggaatcccg agttcatcaa aaagaaaacg ttatgataac taatcagtca aaagactaga
    39901 aaaagtattt cactttttct agtcttttta gttgaaaaac gagagcaaaa ctgttagact
    39961 tacagtcgtg aggttgagat ttccctttat tgatgtgaaa ttcgaatagt taatttcttt
    40021 ttttatcgta tatgggtcgt tttaagtcta cattggacgt aaagtgtcca actaaggaga
    40081 tcgctatgct ggatgaaata aaaatgattg aatctgttgc accagatatt atcgaagtgt
    40141 tgcaagaacg ctataaaatt ttacgaaata tctattggat gcagccgatt ggacgtcgaa
    40201 gtttatcgga aagtatgggg atcacagaac gagtattgcg aacagaaaca gatcttttga
    40261 aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac gctgacaact aagggcgaag
    40321 aagtttatca aagccttgaa aacttcatgg atcagctttt aggtacgcat caaaccgaac
    40381 aacagttggc agaatatttt ggtaaccaac gctgtatcgt tgtttcagga aacagcgaag
    40441 agcagaccaa agtggctgat gcattcggcg aagcgctgag cgaagcccta gaccgactac
    40501 ttccagaagg cgagaatatc atcgctgtga tgggaggaac tactatggct gtggtagccg
    40561 aacaattgtc taatctggaa aataagaaac ggcataacct gtttgtccct gcaagaggcg
    40621 ggattggtga agcgataact gtacaggcaa attctgtcag tgcaagaatg gctgctaaag
    40681 caaacggtaa ccaccgtgca ctttatgttc cggaacaatt aagtctggca acttacaatt
    40741 ctttactcaa tgagccatct atccaagaag tcttaaactt gattagtgaa gcgaattgtg
    40801 ttattcatag tattgggcgt gcattacaca tggcagcacg tcgtaagatg actgaaaaag
    40861 aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc ttttggctac tttttcaatg
    40921 aaaaaggtga agtcgtttat aaggtcccta gaatcggcat agaattgcaa gatttggaaa
    40981 aagtcccgat catcatggca atcgcgggcg gaaaatcaaa agctaaagca attcgagctt
    41041 atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga tgaagctgct gcaaatgaga
    41101 ttttaaaagg ggtaaccctt taaaataaat atttttttga ttttcataag gaggaaatct
    41161 taaatgacag ttaaagtagg tattaatggt tttggacgta ttggacgtct agcattccgt
    41221 cgtatccaag atgtagatgg aatcgaagta gtagcaatca acgacttgac agatgctaaa
    41281 atgttggcac acttgttaaa atacgacaca actcaaggac gtttcaacgg aacagttgaa
    41341 gttcatgaag gttcattcaa cgtaaacgga aaagaagtta aagtattagc taaccgcaac
    41401 ccagaagaat taccatgggg cgaactaggc gtagatatcg ttctagaatg tactggtttc
    41461 ttcacttcta aatcagcagc tgaaaaacat ttaacagctg gtgctaaacg cgttgttatc
    41521 tctgctcctg gcggaaacga tgttccaaca atcgtttata acacaaacca cgaaacatta
    41581 actggtaaag aaacagttat ctcaggtgct tcatgtacta ctaactgttt agctcctatg
    41641 gctaaaacat tgaacgacaa atttggtgtt gttgaaggat taatgacaac tatccacgct
    41701 tacacaggtg accaaatgac tctagacgga cctcatccta aaggtgactt ccgccgtgca
    41761 cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg ctgctaaagc tatcggttta
    41821 gtaattcctg aattgaacgg taaattagac ggagctgctc aacgtgttcc tgtaccaaca
    41881 ggttcattaa cagaattagt aacagttctt gaaaaagaag taactgttga cgaaatcaat
    41941 gcagcaatga aagaagcttc aaacgaatct tacggataca acacagacga aatcgtttct
    42001 tcagatatcg ttggtatgac ttacggttca ttatttgatg ctacacaaac taaagtaatg
    42061 acagttggcg acaaacaatt agttaaaact gttgcttggt acgacaacga aatgtcatac
    42121 actgcacaat tagtacgtac tttagaatac ttcgctaact tataagatag acaaacgtac
    42181 aagtgtaaat tatagacatg tacatttaaa agcggggaag caatcgcgct tcttccgctt
    42241 tttttgataa taagtttatt caggaggaaa taaaatggct aagaaaacag tgaaagatat
    42301 cgatttaaaa gacaaaaaag ttcttgtccg tgttgacttc aacgttcctt tgaaagacgg
    42361 cgtgatcact gacgacactc gtatcaaagc agcattgcca acaatcaact acgttctaga
    42421 acaaggcgga aaagcaatcc ttttctctca ccttggacgt gtaaaaacag aagaagataa
    42481 agaaggaaaa tctttagctc cagttgcaaa acgtctaggc gaattattag gtaaagaagt
    42541 aacattcgta cctgaaactc gcggagaaca attagaagaa gcaatccgca acatgaatga
    42601 cggcgacgtt gtcgtattcg aaaatacacg atttgaagat attgacggta aaaaagaaag
    42661 caaaaatgat acagaactag gtaaatactg ggcttcatta ggcgatgtat ttgttaacga
    42721 tgctttcggt acagctcacc gtgcacatgc ttctaacgta ggtatcgctt caactggtat
    42781 cccaacagtt gctggattct tgatggaaaa agaaatcaag ttcatcggtg aagcagtaga
    42841 agaaccaaaa cgaccaatga ttgcaatcct tggtggcgca aaagtttctg ataaaatcgg
    42901 cgtaatcgaa aacttgattc ctaaagcaga taaaatcttg atcggtggcg gaatgactta
    42961 cacattctac gaagcaaaag gaatcaagat cggtaactct ctagtagaag ctgataaagt
    43021 ggaactagca aaagaattaa tcgaaaaagc aggcgacaaa cttgtcttgc caatcgacaa
    43081 tgtctgtgca ccagaatttt caaatgatgt tgaaacacaa gtaatcgaag gagacattcc
    43141 tgatggtttg atggctttag acatcggccc agcatcagta aaattatttg ctgatacatt
    43201 acaaggtgca aaaacagttg tttggaacgg acctatgggt gtatttgaaa tgagtaactt
    43261 tgctaaaggt acaatcggtg tttgtgaagc aatcgctaac ttagaaggcg caacaacaat
    43321 catcggtggc ggagactcag ctgcagcagc agaacaatta ggatttgctg acaaattcac
    43381 acacatctca acaggtggtg gcgcaagctt ggaattgctt gaaggtaaag aattaccagg
    43441 actagcagca atcaacgata aataaacgac caagtttgct caattctgac gagtaatcga
    43501 tcaaaaggtg aagggagttt tcatgactcc ctagccattt ttcaataaat taaggagtgt
    43561 gttcctcaat gcgtaaacct attatcgcag gtaactggaa aatgaacaaa actgcatcag
    43621 aagcaaaaga atttgctgaa gcagtaaaaa acaaaatccc ttcaaatgac aaagttgatt
    43681 cagtcatcgg atctcctgca ttattcttac aagaattaat gacatcagct gaaggtacag
    43741 aactaaaaat ctcagcacaa aactgctact gggaaaactc aggtgcgttc actggtgaaa
    43801 cttcaccagc agctcttgct gatctaggtg ttcattatgt gatcatcgga cattcagaac
    43861 gtcgtgaata tttccatgaa acagacgaag acatcaataa aaaagcaaaa gctatctttg
    43921 caaatggtat gacaccaatc ctatgctgcg gcgaatcttt agaaacctat gaagcaggca
    43981 aaactgctga atggatcgaa ggacaaatta cagctggttt agctgatctt tcagctgaac
    44041 aagtgtcaaa catggttatt gcttatgaac caatctgggc aatcggtact ggtaaatcag
    44101 ctgacgcaca aatcgctgac gaaatttgtg gagttgtacg tcaaacagtt gaaaaacttt
    44161 atggaaaaga agtatcagaa gcagttcgta tccaatacgg cggttctgta aaaccagaaa
    44221 atattgctga atacatggca aaagaaaatg ttgacggtgc tttagtcggt ggcgcaagct
    44281 tgcaagcaga ttcatttttg tcattgttag acgctgtaaa ataattgttg aaaatttgcg
    44341 aaaaatgcaa gaaatgtttg cactaatgag caaaattaac taaaatcgta tatgagggaa
    44401 atcccttagg aaaaaacaaa ctcaaaggag agacaaaaca tgtcaattat tactgatgtt
    44461 tacgctcgcg agatcttaga ctcacgtggt aacccaacaa tcgaagtaga agtatacaca
    44521 gaaagcggag cttttggccg cggaatggtt ccttcaggtg cttcaactgg tgaatacgaa
    44581 gcagttgaat tacgtgacgg agacaaagct cgttatggcg gtaaaggtgt aactaaagct
    44641 gtcgacaacg taaacaacat cattgctgaa gcaatcattg gctacgatgt tcgtgaccaa
    44701 atggctatcg acaaagctat gatcgcttta gacggaactc ctaacaaagg taaattaggt
    44761 gcgaacgcta ttttaggtgt ttctatcgct gttgcacgtg cagctgctga ctacctagaa
    44821 gtacctttat accactacct aggcggattc aacacaaaag tattgccaac accaatgatg
    44881 aacatcatca acggcggatc acatgctgat aactcaatcg acttccaaga attcatgatc
    44941 atgcctgttg gcgctccaac attcaaagaa gctctacgta tgggtgctga agtattccac
    45001 gcattagctt caatcttgaa agctcgcgga ttagcaactt ctgttggtga cgaaggtgga
    45061 tttgctccaa accttggttc aaacgaagaa ggttttgaag taatcatcga agcaatcgaa
    45121 aaagctggct atgtacctgg taaagacgtt gttcttgcta tggatgctgc ttcttcagaa
    45181 ttctacgaca aagaaaaagg tgtttacgta ctagctgact caggcgaagg cgaaaaaaca
    45241 actgacgaaa tgatcaaatt ctacgaagaa ttagtttcta aatatccaat catctcaatc
    45301 gaagatggat tagacgaaaa cgactgggac ggattcaaga aattaactga cgtattaggc
    45361 gacaaagttc aattagttgg tgacgacttg ttcgtaacaa acactcaaaa attatctgaa
    45421 ggtatcgaaa aaggaattgc taactcaatc cttatcaaag tgaaccaaat cggtacatta
    45481 acagaaactt tcgaagctat cgaaatggct aaagaagctg gctacacagc agttgtatct
    45541 caccgttctg gtgaaacaga agattcaaca atctctgata tcgctgttgc aacaaatgct
    45601 ggccaaatca aaactggttc tctatcacgt actgaccgta ttgctaaata caaccaatta
    45661 ctacgtatcg aagaccaact tggtgaagtt gcagaataca aaggtttgaa atctttctac
    45721 aacttaaaaa acaaataatt cttttctaat gaagatacat gatcgactat tgaaagatag
    45781 tcgagtatga tcacttagtt aagtaatcga actctctact tgctgaaata tcagcaggta
    45841 gagagt
//
LOCUS       DAZKOK010000009        45670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000009 DAZKOK010000000
VERSION     DAZKOK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.009"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..245
                     /locus_tag="ACG90W_000460"
     CDS             6..245
                     /locus_tag="ACG90W_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476854.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            312..521
                     /locus_tag="ACG90W_000461"
     CDS             312..521
                     /locus_tag="ACG90W_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476855.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            581..1252
                     /locus_tag="ACG90W_000462"
     CDS             581..1252
                     /locus_tag="ACG90W_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0476856.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1305..3281
                     /locus_tag="ACG90W_000463"
     CDS             1305..3281
                     /locus_tag="ACG90W_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0476857.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3294..4049
                     /locus_tag="ACG90W_000464"
     CDS             3294..4049
                     /locus_tag="ACG90W_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0476858.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4046..4870
                     /locus_tag="ACG90W_000465"
     CDS             4046..4870
                     /locus_tag="ACG90W_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0476859.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4880..5128
                     /locus_tag="ACG90W_000466"
     CDS             4880..5128
                     /locus_tag="ACG90W_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476860.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5171..7270
                     /locus_tag="ACG90W_000467"
     CDS             5171..7270
                     /locus_tag="ACG90W_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0476861.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7308..9365)
                     /locus_tag="ACG90W_000468"
     CDS             complement(7308..9365)
                     /locus_tag="ACG90W_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0476862.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9479..9691)
                     /locus_tag="ACG90W_000469"
     CDS             complement(9479..9691)
                     /locus_tag="ACG90W_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476863.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9878..10345
                     /locus_tag="ACG90W_000470"
     CDS             9878..10345
                     /locus_tag="ACG90W_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476864.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10342..10584
                     /locus_tag="ACG90W_000471"
     CDS             10342..10584
                     /locus_tag="ACG90W_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0476865.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10602..10904
                     /locus_tag="ACG90W_000472"
     CDS             10602..10904
                     /locus_tag="ACG90W_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476866.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10976..13030
                     /locus_tag="ACG90W_000473"
     CDS             10976..13030
                     /locus_tag="ACG90W_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476867.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13030..15642
                     /locus_tag="ACG90W_000474"
     CDS             13030..15642
                     /locus_tag="ACG90W_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0476868.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15639..18170
                     /locus_tag="ACG90W_000475"
     CDS             15639..18170
                     /locus_tag="ACG90W_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0476869.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18222..18554
                     /locus_tag="ACG90W_000476"
     CDS             18222..18554
                     /locus_tag="ACG90W_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476870.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18555..19148
                     /locus_tag="ACG90W_000477"
     CDS             18555..19148
                     /locus_tag="ACG90W_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476871.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19311..21005
                     /locus_tag="ACG90W_000478"
     CDS             19311..21005
                     /locus_tag="ACG90W_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0476872.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG90W_000479"
     CDS             21693..23570
                     /gene="ltrA"
                     /locus_tag="ACG90W_000479"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0476873.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23701..24078
                     /locus_tag="ACG90W_000480"
                     /pseudo
     CDS             23701..24078
                     /locus_tag="ACG90W_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24078..25283
                     /locus_tag="ACG90W_000481"
     CDS             24078..25283
                     /locus_tag="ACG90W_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0476874.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25299..25943
                     /locus_tag="ACG90W_000482"
     CDS             25299..25943
                     /locus_tag="ACG90W_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476875.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25954..26760
                     /locus_tag="ACG90W_000483"
     CDS             25954..26760
                     /locus_tag="ACG90W_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476876.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26782..27012
                     /locus_tag="ACG90W_000484"
     CDS             26782..27012
                     /locus_tag="ACG90W_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476877.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27558..27833
                     /locus_tag="ACG90W_000485"
     CDS             27558..27833
                     /locus_tag="ACG90W_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476878.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27875..28297
                     /locus_tag="ACG90W_000486"
     CDS             27875..28297
                     /locus_tag="ACG90W_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476879.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG90W_000487"
     CDS             28316..29908
                     /gene="mobP2"
                     /locus_tag="ACG90W_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0476880.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29920..30255
                     /locus_tag="ACG90W_000488"
     CDS             29920..30255
                     /locus_tag="ACG90W_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476881.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30402..30596
                     /locus_tag="ACG90W_000489"
     CDS             30402..30596
                     /locus_tag="ACG90W_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476882.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30628..33000
                     /locus_tag="ACG90W_000490"
     CDS             30628..33000
                     /locus_tag="ACG90W_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0476883.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33061..34521
                     /locus_tag="ACG90W_000491"
     CDS             33061..34521
                     /locus_tag="ACG90W_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0476884.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34532..36736
                     /gene="topB"
                     /locus_tag="ACG90W_000492"
     CDS             34532..36736
                     /gene="topB"
                     /locus_tag="ACG90W_000492"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0476885.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36752..36901
                     /locus_tag="ACG90W_000493"
     CDS             36752..36901
                     /locus_tag="ACG90W_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476886.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36975..37568
                     /locus_tag="ACG90W_000494"
     CDS             36975..37568
                     /locus_tag="ACG90W_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0476887.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37584..38483
                     /locus_tag="ACG90W_000495"
     CDS             37584..38483
                     /locus_tag="ACG90W_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0476888.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38486..38971
                     /gene="ssb"
                     /locus_tag="ACG90W_000496"
     CDS             38486..38971
                     /gene="ssb"
                     /locus_tag="ACG90W_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0476889.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39337..39597
                     /locus_tag="ACG90W_000497"
     CDS             39337..39597
                     /locus_tag="ACG90W_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476890.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40038..40244
                     /locus_tag="ACG90W_000498"
     CDS             40038..40244
                     /locus_tag="ACG90W_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0476891.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40244..40495
                     /locus_tag="ACG90W_000499"
     CDS             40244..40495
                     /locus_tag="ACG90W_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476892.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40510..40947
                     /locus_tag="ACG90W_000500"
     CDS             40510..40947
                     /locus_tag="ACG90W_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476893.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40940..41647
                     /locus_tag="ACG90W_000501"
     CDS             40940..41647
                     /locus_tag="ACG90W_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476894.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41812..42012
                     /locus_tag="ACG90W_000502"
     CDS             41812..42012
                     /locus_tag="ACG90W_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476895.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42028..42207
                     /locus_tag="ACG90W_000503"
     CDS             42028..42207
                     /locus_tag="ACG90W_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476896.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42548..42811
                     /locus_tag="ACG90W_000504"
     CDS             42548..42811
                     /locus_tag="ACG90W_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0476897.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42805..43155
                     /locus_tag="ACG90W_000505"
     CDS             42805..43155
                     /locus_tag="ACG90W_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0476898.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43179..43793)
                     /locus_tag="ACG90W_000506"
     CDS             complement(43179..43793)
                     /locus_tag="ACG90W_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0476899.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44107..44529
                     /locus_tag="ACG90W_000507"
     CDS             44107..44529
                     /locus_tag="ACG90W_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476900.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44539..44742
                     /locus_tag="ACG90W_000508"
     CDS             44539..44742
                     /locus_tag="ACG90W_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476901.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44953..45537
                     /locus_tag="ACG90W_000509"
     CDS             44953..45537
                     /locus_tag="ACG90W_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0476902.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16743 a         7205 c         9261 g        12461 t
ORIGIN      
        1 agtagatggc gcgaccagtt ttagaggcga tggatatgga tactttgaca aaaggaaggt
       61 atgtacagct gttagctcgt ggttattcac ctaaagaagt attcaaagcc ttatctaaag
      121 gaacagatat ggaaaaagaa agattgcgaa aagaattcga ttattggaga acccataatg
      181 gaatcaagga tttgaaacca gcacggccag taattaaacg aaagaaaaag ataacccaag
      241 actgaagtgt agagcgtatg tgatcatagg cttttttatt tttgcaaaaa acaaggaagg
      301 taggaaaact catgaattat gaagaaatcg aaaacagaaa aaaagtgtcg aaagaaatgg
      361 aagaaaagtt actaaaaacg atgaagcaaa aacaccttaa aagattatct gtcgcgcaat
      421 atatcaatga tatgcagtta acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa
      481 actttgaaca gttgagacga acttatagaa ttcattttta aaactttttc tatggaaatg
      541 agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg atgaaaaaat ggttatttgg
      601 ttttttagga gtcactttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa
      661 acatgaagga gaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa
      721 tgcacatgca aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg
      781 gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc gtcaataaac aagtatttgc
      841 acaaattcag attcctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata
      901 cactttgagt cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa
      961 ctatgtccta gcaggtcata acatggaaat gccaggtgtt ttattttctg atattcaaaa
     1021 gttgtcatta ggggaggtga tggacttagt tagtaacgat ggcgtctatc gttacaaggt
     1081 aacgcgtaaa tttatcgtac ctgggtactt taaattaatc gatggcgttc ctgaagagaa
     1141 ctctttcttg tctttgccaa agaaaggaga gaaaccctta ttaacgttgt ttacttgtgt
     1201 ttatacgtca caaggaaaag aaagatacgt ggtacaggga gagctacaat aacaaggttt
     1261 taatcaacgc gagtcgttga aatatgaaat gaggagagta aaaaatggca ataaatcggt
     1321 atgtaaaaaa agctggaatg cttgttggaa tgttaggaat cattgcaggc tgctttcagg
     1381 ggtatcaagt acatgcagaa gatgtcacgc aaaaaacacc accagagaag gtcaatatca
     1441 cggttcataa attaatgtac gatcaaggaa cgcaattgaa tgttgatatt gacgggatca
     1501 aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt aacgaaatac aacaaggcag
     1561 attatggtga tgttgagttt acgttaggaa atattacgga tcaagtatta ccgagagaag
     1621 attctgatct aaccaacgcg aaagtggacg agattgtaca agatgtagaa gacaagggat
     1681 ctaacagtga gtacgtcaag aatgcaacca acaaaattac ttcagctgta gatgaaaatg
     1741 gtgagatcac ttttactgat caaccagcct atgtcaattc caaagggaat gtatatgtgg
     1801 tttatgaaag taaatccgct gctggattag tcacacaaaa agcaaaacca atggttgtga
     1861 ttgcgccaat gacagataaa acaggatcag gatttttaaa aaatattcat ttgtatccta
     1921 agaacattgt ttctaagttg agttttgaat taacgaaatt tggtgacgat ggcgcagacc
     1981 aaagcaaaca aaccctttta aaaggggcaa aatttgagtt atacaagggc gaacctggaa
     2041 aaggaacaaa attgggtgat cttgtatcgg atgatcaagg aaagctgact gcgactgatt
     2101 tgacgttagg aaaatactat tttgtggaag ttccttcgga agttgtagtg ggatcagatc
     2161 aagaaccaac agcagatcaa tatttattag gcgcagacgc tcgcaacgat gcgcacaata
     2221 aattaacctt tgaaattacg aatgatggcg tgacaagtga cttaaaagct tcatacgtaa
     2281 actataaagc accagtgatc gataaaacag taacaaatgg aacaggacaa gaacattctt
     2341 tccaaattgg tgacgcagta aattatcaag gaacgattca tattccaaca gacattgcag
     2401 gtggcgcaga cggtatcaca gtaaacggtg tgaaaagcga aacttctcct tacagtgtat
     2461 ttaaatgggg agatacagca ggtaaaggac tttcttatgt tgctgaaaaa gcagatatta
     2521 aagtaacaaa taatgatggt tcagtcgtat taaaagaaaa tactgactat aaaatcgaaa
     2581 attctgatag tggttttgtg atcgatttta tcgtaaataa tggacaagta agtgatacag
     2641 ttgcgaattt acatggtcaa gatctaaaaa tgacttataa catgtttgtg aatgatagtg
     2701 ctgaaatcgc aaatccatta acaaatagcg tggatttcgt ttataacaat aatccattta
     2761 atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt gacttatggc gcaaaattct
     2821 taaaagtcga tagcggccta tttggaacag gtatcaaagc aacaccgctt gaaggcgcag
     2881 aatttgcagc taagaatgca gaaggtaagt actacggtgg acttactgat acagataaag
     2941 atggtgtcaa agaagcagtt tgggtagacg atgtagcaaa tgcagcaatc ctaaaatcag
     3001 ataaagaggg tcattttgaa atcactggtt taactgaagg agattactca ctggaagaaa
     3061 caaaagctcc tgaaaactat caaaaattaa caaaagagat ctctttcaaa gtagacaaag
     3121 attcatacaa agaagaaaac cgaatcacga tcaaaaataa tcaaaaagct tccgtaccaa
     3181 tgactggtag caacggtttc caatcttacg tattgatctc ttgcttgttg ctaggagcag
     3241 ggggactttc tgcagttgtt tactttaaga agaaagccta gaaggatcag ctaatgatta
     3301 ttcgtaagcc cccaaaaaga agagtcatgc acaaactgat gatatttttt tggttgctgt
     3361 cggcagtagc agtctattta attccagttg tggaaacaaa tgtagcgaaa tttcaacaac
     3421 agcaaagaat tttagccatt gaaaatcttg aaaaaacaga ttcttctttg gatgttgcga
     3481 aaatagagtt aggcgatcct gttggtgttc ttacaattcc aagtatttct ttaaaactac
     3541 caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt acggaaggaa
     3601 ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcggggcac agtggacttt
     3661 ataaagataa tcttttcgat gatttgcctt cagtaaaaaa agggcaaaaa ttctttatta
     3721 aggtaaatga agaacaacat gcctatcaaa tttatcgaat tgaagaagtt aaagcagaag
     3781 aattacaaaa aaattattta gattatctta tgcctgaaaa agaccaagat aggatcacgt
     3841 taatgacatg cacaccaaaa ggtgtcaact cacaccgctt tttagtgtat ggtgaacgag
     3901 tttccttcag taaagaagat ttaaaactac agaaaaaagc aaaaaatagg aaatcaattt
     3961 cattggattg gcgattagca ctgctattcg cttttttttg cgttcctatt atttttattt
     4021 ataaaaaaat aaggaggaga tcggaatgaa taagagaaaa tggctgtttg tttgtgtagc
     4081 agctctcttt tctactgtag cttattcgca actgtctttc gctgacagca gtgatagtag
     4141 taccactcaa acaatagata attcaattac tgcaccaagc gaaacggaca gttcggagaa
     4201 tactacagaa gaaacggcag attcaaatat aagctttact gaatcaacga atccaacgtc
     4261 agaaagttct tcagagactt cagaaaagcc gcaagacgta gaagatacgc aagtgatcaa
     4321 gcaagccatt catattcaaa aaattatttc ttcgaatcaa atcaacaatc aaggcagtca
     4381 agtagcccag caagaaggta tcaatggcgc gaagtttatt gtctacgata tcacgaatat
     4441 cttagaagaa atgtcacaag atgataatga gaaaaatcaa tctgtagaaa taattgagtc
     4501 aaaattgaaa gatcgagcaa aaaaactttc atacgaccag ttgaaaaaag tggctgaagg
     4561 agaaaccaaa acgattgatg atcaggaagg cgtgattgaa ttttccattg aagtacctgc
     4621 aaacaaaaaa caagcatact atatcgttaa tgaatcaagc cctgaaaaca tttcaaacag
     4681 tgaagatatt gttttattga cacctgtttc aaatgaaaaa ggtgaatttt tgacggatgt
     4741 ttggttatac cctaaatcag agaaatcaga gccgaaagaa gaaattaaaa aagttgtgtc
     4801 aactggtgta aagaagaact tttttgaaaa ttgttgggaa ttttttgctc aattattttt
     4861 tgataattag gatgaaatga tgaaaataat aagaatatta gtttttaatc tatccttttt
     4921 agccgcaaca atattgtgtt gcggtttaat tttttctcaa ttatcaattt atccaagata
     4981 tcaacgattg ttcattcatt ttctaaacat tcaatgtgct tccatgccat tagaggtgat
     5041 taaaaagctc ttgatcaatc caatttttat ggttagtttg gttcttctta caggattggt
     5101 aacgtgggta ttaggaaaaa aagaatgaaa agcttacgat cgtttaaaaa aatattagat
     5161 ggagggcatt atggaaaata aaattcattg cactacggga agagtaatta ctttagtgtt
     5221 gtttttgttc cttcttcttt ttgcgagggg aactgcagtc caggccttag aaaaagttga
     5281 tccgagtcaa atcaaagaaa ataataatct ggtgctttat catgatagtg attcattgag
     5341 aaatatcgaa gtaactggga tcaatggatt taagatacta gatgagtatt caactaaaaa
     5401 acaaaaagta cttgcttggc caaaagtcaa agatttgaaa agtacgtcaa aaatcgttgc
     5461 ccattatcaa aaagtaggta cttatctagg aaaagaaatt gattgtgacg tgactttttc
     5521 taattttgta gcgcacgctg gggttaacca agacggatta gctaaaacaa ttaatggcaa
     5581 aaagtattat aattatttgc tctttgataa aaatatttat cgaggatttt tcatgcagtc
     5641 aatcaatgtc atgaatgcaa aactagaatt taagtatgca ggaacaaaaa caaaagtagc
     5701 tttgagtgaa ccacagccag aaattcaatc aaaaatgaga tcgatgagag cagctggtat
     5761 tgatttaaac gatacatttc tatccattta cagtttaaat ggacagtatc agcaacgtgt
     5821 gtcttggtat aatgacaata atgaatctgt cggtgaacgt aatattgata tgagagaatt
     5881 tgttgggtat gaaaaaatgg gtagcttacc ttattttgtc acaacagata cagcatgtgc
     5941 agaatacaaa gctcctgcgt tatcaacaaa caatttaact tcaaaagtag tgggaggacg
     6001 tgcagaaaag gcttatagct cgaatgatca tttcaccgat gttgtaggag ctgatactta
     6061 tcacagaagt ggtgtaacgt atacgcttca aggcgcttcc ccaacattca tgattggcgc
     6121 aaatacgaat agtatgatgt ttagctttga tactgcattg ctatggacac cacaaccatc
     6181 gaagcctaca aaagaagtgt ttaacaaagc taatactgaa gaggcagcac acaatattga
     6241 caaaaaagtg attccacaag gatcagatgt ttactatcat attcaccaaa agtttgatgc
     6301 attaacagtc aacacaatga acaaatacaa atcatttaaa atcactgata cctttgacag
     6361 caaaaatttt gatatggtat cggatgggaa aaactatgat ggcgcattgt ggtatgttga
     6421 agcgaatggt acttggaaaa aagctgatac aggaggaaag ttaactgtat caggtaatac
     6481 agtcacttat actgcttctg acagcttttt agctaacgct gcaaattacg gtagggatta
     6541 tattttgcag ctccatatga gagcaaaaaa tgatgcaaaa ggaattgcac caaatgtggc
     6601 tactgtaacg tttaacaatg atccgaacaa aggaacacaa aaaacaaata cagtagaaaa
     6661 ctatttctct cttaaaccaa ataaaaaagt ggaacaagat gggaaagatg ttgatggtcg
     6721 aaatgatggt aaaaaaggaa cgcctacagc tcctttaaat gcaggatctg aagtacaata
     6781 tctagtcaca caaaaatggc atacaaaagg tgtagacatt gcctcagatc attacaagca
     6841 atttagtatt caagatccag tggaagatcg tttgacgtat aaagaaggtt ctgcgcgagt
     6901 gatagatatg agtacaggaa aagatattac ttcagaaggt acactaacct atgatagcaa
     6961 ttcaagaacg ctgaagtggg aagcttccgc tgatttctta agtaaaaatc ttttagatgg
     7021 acgagaaatt cagctgatat ttacagctaa aactcctttg aatgaagaat tggatatcga
     7081 caaccaagca aaagtttcag tagaaagtat tgagaaagca actaatgtag taacgattgg
     7141 ggtaaaacct aacttacctc agatcattgt gccaaaaact ggatcgaatc atttagtgct
     7201 aactgttatt ggaagtatat tatttcttac tttagcatca ttgggctatg tagttttaaa
     7261 actcaaataa atataaaaac aaggctgaga atcagccttg ttttctttta tttgaagatt
     7321 ggattcgcac taataaatac attatctacg ccaattctac cagcagagtc aactacaaaa
     7381 ttatgaccta atccgagaat actttggtta ttataaagta aatttgcatt atgggcaggg
     7441 ctatttttcc atcctaccat ggcatcgata gcaccatcgt ctgataaaag gtcatcaata
     7501 tacagcccag tttgacttat gttttctccc ttagcaccgt agcagcctaa gctttccaat
     7561 aaatcttgga aagtaccacc accagggcga tagtgactga agctgacttg taattcttta
     7621 gctcgaatat ctgcagcctg ttgaacaata tcattgaatt gcattggggc aacaccattt
     7681 tgttggcgat aatcatttaa ccaagcaagt tctttttgac gcataaagtt acgtaattcg
     7741 tctaatgaaa tctttttata tttgaatgtg attgttttat tttgatcgac gttattaatg
     7801 gatcgttggc cgtttttatc cacggtaacg gtaggatcgt catttctgca caagaaataa
     7861 ttttgatcag tcatgttttt ccaaggagta gcaaagctat ccccttcgta tactgttttt
     7921 gattcactag agagaacagc gttggtatca gcatttacat gattaacagt cacattaaat
     7981 ttacgagtgt agttgaaaat ataattttga ctttcagtca cattgtttaa ggttactgaa
     8041 tttgttggtt ctacccgatc gtctaaaaca tatccttgaa tagaaggagc agttaaagtg
     8101 acactgtcac cgtgagtgac attgactatc tgttctttca aaatattaac tcgtccttcg
     8161 tttttgtagt tgttattgca caacaaattg atttgatgca cttgattata attgaatgtg
     8221 attgttgtat tacctgtgat tgttttgctt tgagtatcat taccaatcac tttgtagcta
     8281 aaattatctt tagcgccttc gctatattta aatgcttttg ccttagcagt gtaagtagaa
     8341 ccagaagtta cttttgcttg atctgtagca atcacttgac ctgtttgatt gtcaatatgg
     8401 ttaatagtaa ccgtatattc ttttggtgtt tcaggtttgt gttgcaaccc tttaaatgca
     8461 gcatcaagac tatttttagc attgtttact tgtgtttgag aaaccttaga atcgtctagt
     8521 acttttttag cattggataa agcagtctga aaattattcc aagattcttc tgtaaaatct
     8581 cctttagcag ttgattttac tcggttgtat aaagcttgta attcagcttt attcacagta
     8641 ggaactggtt ttttctttaa tcctttgtaa gcagaatcta atttattttt ggcactatcc
     8701 acttgctttt gagtcgcttt tgagtcattt aacactttct tagcattgtt taaagcggtt
     8761 tggaatgcgt tccaggtatt gtcggtatag tcaccttttt tcgttccctt aacgctcgta
     8821 taaagagctt ctagagccga tttattcact ttgggtttag aggtgtcagc gccataccaa
     8881 ccgatacctt cgtatttcca gccatagtct ttggttaacc attgtacttc tttccaactt
     8941 gcagtgtagt gatgtgagcc agattttaca ccaggaagga aaaggcggta gatcggttta
     9001 ttattttttt cggcagaacg ccaggcaggc ccttcataag tccagccgta atttttagtc
     9061 aaccatttca cttcatccca actagctgtg taatggtgat cacgaagacc tttattgtac
     9121 aaacgataga caagatggcc accgctagtc ggtgcttccc aggcggggcc ttcataattt
     9181 ccccatttta tctttactag atggtcacgt tcttttttgc tagaggtgta aaaatgttca
     9241 ttattatttg gattgtaaag gcgatacatg acgttagttg aagcagcgtc aacagatcca
     9301 gcaagactaa gtgaggcagc aaatgttcca atagctaaaa cagtttttgg attaaataat
     9361 ttcatttttt caactcctaa atttaattaa aaatgtttac atgattatta tatatcggct
     9421 aaccgatata gtcaataaaa aataagaaaa gccttatata attttggctt ttcttatttt
     9481 atttgttagt tgaagtatta atatcaacga tttcttcagc caatttattt aatttcatag
     9541 catttatgaa tctcatgttg tctatactag agcgtttgtt ggcaaggtca cttactgtag
     9601 ttaagggtac gccagattct atggatatag aacgaactga tttaccacta tcaaacagca
     9661 accattcgat tttcttttta tcagcgtaca tttcaatgct ccttcttgtt ttttctttat
     9721 tatagctctt ttttgtaatg aatcacaata tcaagagaaa aattttctat agaaaggtgg
     9781 atttttatga gtattcgttc tccgtgtatc caaatgaggt ttgttgctac tttcgttatt
     9841 tgatatacta aaaataaaca acgaaagaag gaataaaatg aagaaaaagc aactatggtt
     9901 tgtctctatg tttgcaatat tttttattat tttaagtgga tgttcaagta ataagaaatc
     9961 ggaagctaaa gaagataata gtttattgca accaggaact gtttggaaag aagaagtagg
    10021 tggtcttatt cataatttaa aaattataga tgatactacc tgggaatatt ctgaaagtgt
    10081 atggcatcca gatcctgtac aaataacagt taagaggcaa aaagactatg aaggtttaga
    10141 aagatacaaa atagtagatt ctgcaggcat tagggaattt ataaacaaac ctgattcatt
    10201 atttattgtt gttccctatg agaaggatgg cgtaaaaaaa ataatttttc ttggatctag
    10261 caaggatgag aagcgaacga aagagaaatt aattcatgat ggatcagagt taaataaata
    10321 caaattacaa aagactagtg agtgattgct agtcttttta tttatcctga aggaggaatc
    10381 acagtggcaa aagttaaaac accattcgaa aaagccgcgt ctaaaatctt agaacgccgt
    10441 ggtgtttctg aaatacagtg gaaaaaagaa gttcttgaaa aagcacaatt tgacctatta
    10501 cgaggaaaag ataaggactt agaagaatat atctatcaac gtgaacgtga aaaacttgta
    10561 ttcgatgaaa ttattaaaca ttaaaaggag cgaataatct tatgtttgaa tcaattacaa
    10621 tgaatttttt aactcaaccg aaagtagtag ctgcagttga tatttttaga gcattacaaa
    10681 ctgccgcaac tagtttgaaa aataatctga tgatttttgc ggctgtagct gccgttgcct
    10741 ccattgctct tggaggtatt ttcttcttct ttgggcgaag aggagctgaa aacgggaaga
    10801 cacacgtagg ttatgtattg ttgggggtag gagttatttt agctgccgct tctattgtag
    10861 tttcaatgtt ctcaatgttt ggacaaaccc cacaaggact ataaaaaaaa caagtaggag
    10921 gagtaaaaag tacgtggcta ctttttactc ctttttttat gaaaggagaa tattcatgtt
    10981 ttttacgatt tcaggaaatg caaaattgga taaagagtta ataagaaaag caacgaagaa
    11041 agtgggaaag cgtacttcct ttttaccgaa aacaattaaa tattctgtca ttgaaacgga
    11101 agaacagcta catgtgttag gacaagtgtt agaacaagag aatcaatctt ctgttaaatt
    11161 gactctcacg ttacatgata aagaaggaaa aatagaaaag gctggcacag aatacgtttt
    11221 tggcccactg gattatcaaa tttcagaagg tttttatcct acattgatag atttaatccg
    11281 ccttgatgtg tttaaagata cggatgaaac tatgagtgtg gaagagtctt atcgaaacga
    11341 aaaactgcta caagttttag aagaagagtt agagagcgac caaagaaaag tgaatcaagt
    11401 gagtgaagtc gaagtaaatg aagaagtacc atgggaacga ccaatctttc caaagaagga
    11461 gtcaaaagaa aaagagacag tctatgttga cgaacaagct gatattccga ctcagaaatc
    11521 gatatttgag gaagagaacg atgtgatggg tcaattggag gtagatccct ttgaagaaac
    11581 aatggaggat gaagagacgg acgaagtaga agttgatcca gttgaagcta atgaagagtc
    11641 aacatggact gaaggggatt ttcaggaata tattgaacca cttatcagtg aagaagtaga
    11701 aaaaaacgaa acagagatac aaaaggcaac tgtatttgat tatattttgg atcaatatcc
    11761 gccagagcat gaatggatga aagaaaaaaa caaattattt ttaaatgagc tttattcgaa
    11821 atatcaatta ccccaaacct atcaagcatt tttagagagt aaagaagaat taatctcaca
    11881 gggtaaaggc acacttttag aacagttaga gtctgtccaa tcaacaaatt ggcatgagct
    11941 tgcacaagaa gatttattag ccacctttga agaacgtcaa caagaaacag atcaagaaat
    12001 taatgagtat gcgacggaac agtcgtctaa ttgggagaaa gtaaaagagg aactaaataa
    12061 agaagaacag cgagtgattg aagaagaagt acaaaaagtc aaagagcggt acgaaaagaa
    12121 acgcgacaat gcttattcct tgtgtgtaga aaagatcaac aattttacta agacgaatca
    12181 agaagcgtta tcgaaagaaa aagaaaactt gttaaaagaa cgtattaaag aacgaaaaga
    12241 agagttctac cgcggtttac gtacagataa gctaaagatt agcaaagaat taaatgaaca
    12301 actgaataag ctatatcaaa caacgactga agttcttatg gaaaaacacg aacagatcca
    12361 gcaagaatta caagtacaaa ttccaaaatg gaaaaaagag tatgaaattc aacaacaaga
    12421 attgaaagaa caagaagaaa aacaacggca aaaagaaaaa gagcgtgaac aattagaaat
    12481 aagaaaacgg gaacaggatc ttgcggaaca aaaaatggcg atagaaaaac aacggttaga
    12541 gcaagaaaag gaaaaagaag cagctgctcg taaggaacgt caagaacaaa tggatacatt
    12601 gcgtcaagca cagttgaatt atctagcaca acctcaacca gtagctgctg ttcaaacgcc
    12661 agtgaacaac caagcaaatt taactccttc accccagcgt tcctttaacg gatggatggt
    12721 gggatgtatt gcaagtttaa gcttgcttct aggtggagga acagtatttg cttttaatca
    12781 tatcaatcaa tcttcacagg ctgattctgt agccacaacg gcgtcaatca aggaagaagc
    12841 ccaggaacaa gcaagaaaag agtatgaaca acaattagaa gaattattga atcgtaccaa
    12901 tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag agtgatccta gtaaggctaa
    12961 agaaggatca agcgattcaa ctgtacactc aaacacttcc gaaagttcag aatcaaatga
    13021 ggtgaaataa tgcgtattag agaaacaacg agacgaaaag acaaacgatt caatttagaa
    13081 aagaataggt atctttctca tttctatcgc tatactcctt caggagaaga gctaccagaa
    13141 aaacatgaca aacgtatttc tcaaaagatt ttttatagtg gcttagctta tcttccttta
    13201 aactatgttg ttacaagaat accagaagca tttcatcgat ttgagattat tccaggaagt
    13261 ttacaaggag tgaatatttt tcagttacaa gaaatgccgc agttattcca ttttgattca
    13321 actggttttt ggaatccttt gaccccaaca acaagtttag gcacacgttt agttgtttct
    13381 acgatttgtg ttgttccagc tgtacttatg ggattgaaaa tggacatgtg gtttcgtccg
    13441 ctagcagatg gacaaaaagg ggataatcga ttaatgacct taaaagaaat ccaacagatt
    13501 tatccgcaaa tacctgaaac aaaaactact tttccaggtt atggaggtat tccaatcaca
    13561 cattacaaaa aatattggta cattcaaaca gatactgtta atacgtgtat tgttggtacc
    13621 tctcgatcag ggaaaggtca gattgaagtg ttagccacca ttgacaattt atctcgtgca
    13681 gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag aattatatgt tggtagtaaa
    13741 gatgaactag aagctagagg gtatgatgta tatgccttta atatcaccga tccactgcaa
    13801 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaaacgagg agacatagat
    13861 actgcaacac agttaaccaa tactttcaca aatacgattt attatgatgc caacgcttca
    13921 gataacaaat actttaacga aaacgcgcag aaagctgtca atgcgcttat ttttgcgttg
    13981 ttagaacaag ctgatcaaac aggagattac tccaaattga caccgaataa tctcgttgag
    14041 cttctttctg agattggcgg ttttaattat tatccagatc cagacaatga attcaaacag
    14101 ttgaatgcct tagatgaatt tatgaatcag ttgccacctg gaaacattgc aaaaaaacaa
    14161 tatggggcaa cgaaaattgg atctgataaa gcgaagggaa atattttatc aacggcaatc
    14221 acgggattga atccatttac tctaacgaaa attgcaaaga tgacttctca atcaacgatt
    14281 gattataaat cggttgggtt tccaaaatac ttagatttga aattacatga aagtctttta
    14341 aatcaacgag tcgtagtaga attttatcat aagggaaaga aatttcatga agaacgtgtc
    14401 aaagtaggct ataaaggatt tagtgaaatc aattttgatt gtcatttagt tgcaggtgat
    14461 caagtttgtt taaaatacga tcatcaagga aaagaatatg tggcttggta tgaattttct
    14521 caaagagtat tgaaagacga agagggtaat atcgtttatc ggaaaaaacg aggagaaacc
    14581 catttgccag aattagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
    14641 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgcttaca
    14701 cctgattatg atccatctaa ccatatcatt gttagtattt ttcttagtca gttatataaa
    14761 gaattatcta cgcaatgtgt taagactaaa ggagataaat gtcaccgcag gattcatttt
    14821 attttagatg aatttgggaa tatgccacca atggataata tggaaggaat catgacggtc
    14881 acagctggcc gaaacatgtt gtgggatctg tttattcaat cttatcagca actttatgcg
    14941 aagtatggag aacaaaacgc agcgattatc aaagaaaatt gtcagacaca tatttatatt
    15001 atgtcgacag atgataatac catcgaagaa ttttctaaaa aagtggggaa taagacggtt
    15061 gaacaagaga acagtaccat caattcatta ggtatgaata ctcatatcaa tcgcaatgtt
    15121 gattcagatc gtatcttaac tcctgaaaga atttctactt ttttagaagg agaaacgatt
    15181 gtactcgctc ctttaaaacg acaagatttg aaaggtcata aaatacggcc gtatcctatt
    15241 tttaatcatg gggaaacgaa tcttccttat gcttatcaat atattgaaga tttcaatcca
    15301 tcgtgggata tcaatgatat agacatacat tgtacacatg cggacttgga tcttaaggct
    15361 ttacaaattg attatgatta tttcctacca gatgaatatg ctagaaatct ctatcatgga
    15421 ctgcatgatc aattagaaga aaaaaacgaa gaaaatatgt tttataaaat cgttcaatct
    15481 tttgagaatg agacggctaa aaaattgtta attcaatttt acgaagaaca agcagcagat
    15541 cgacttcaag aatatgttga aaagttgatc aaaaataaag aaatttctga aatgattggg
    15601 aaacggttgt tattggaatc tgaaaaggtg gtgaaagaat gatcattgga ggaatttttt
    15661 ctggcatagc cgactggtgg tcaggcttat tcggagaagg ggatgcaaac cccaaaaccg
    15721 taacgaaagt cttacaagat tatgggaatt atctgcagta tcaagatgta tttagttgga
    15781 tttggaacac ttttctgtgg ggattgatta aaggattata taatctcaat actttactag
    15841 aaaaaaccat ttatcaaagc tttgatttaa aagacttatt gaacgccgct ggggtaaatg
    15901 cactatttca aagtttgatt agtaaggtat tggctctttt ttgtgtcgtc acattaattt
    15961 atttaggctt gaaatttttt atgagtaaac acccaccaaa agtcaaaaat atcttggtta
    16021 atttatttat ggccatgatc ttgatttttg gtggtagttc tttaatcgat gaagggttaa
    16081 atatcagtaa aagcttttac ggagacgtca ctgcagcaaa taaagcagat aaaacgggat
    16141 cacctgcttt tcaaattatt caaaataatg tgatcgatgt cagtacgtta ctagcaactg
    16201 acccgaataa agtagaatcc ctaccaacag ataaaagaaa tgctttaaca gcgaaaaatt
    16261 ttaaaacagc gaatatcaat gggattatta ctcctgaaca agctaaaggt atggcaggta
    16321 atgataaaag tgggttatca gatatacaaa aggatcggtt aaaggcatta caataccgat
    16381 tggaactaga tgataatggg aaagaaattc ctgttcaaat tagtgacgaa ggcttagcaa
    16441 aatatgtcta tacttcaggg tatcgtcgat acagtagtaa accaggtatt attttagctg
    16501 gtgaatcttc tttggcagta gcctatttat ttatcctatt tacgatcgtt acttgcatta
    16561 ttgagctggt atttaaaaaa ttctatttag tgattgccgc gtcaacggac tttgagacag
    16621 gtcaacggat gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta
    16681 cgattttaga attacgaatc tataccttaa tgctttcagg tatcggtgat ttgcatgcgg
    16741 caggaaagat caatggattc ttgtatgtcg ttgctttgat tgtactaacc attgcgctat
    16801 tcaaaggctc tcaatcagtt acgaaaattt tcggcgttga tacaagcctg aaaaacggca
    16861 gtaattctct tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag
    16921 ctaaaaacct tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaaa
    16981 atggatttgg ccatcgaaaa gatgttaccc ctgataacgg ggaagaacaa tcagacgata
    17041 aagctacaac tgtacctaat agtaagaaaa cagtttccga aatgttcggg aaagcgaaaa
    17101 atggtgtttc taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa
    17161 cagctgaaga tcttacagaa aaagccaaag acaccttaga agataaatta gacggcacga
    17221 gagctaaaga attggctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag
    17281 aaaaagcttc agaagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg
    17341 cagcagtgaa agctaataca acaaccgaaa aagaaaatca agaaggtata gtgattcctt
    17401 cagtcaaaga tcaccaagaa gcgttgcact ctaatgaaaa ttcgattgaa gagaaaaatt
    17461 ccatagaaga aagaaattca gaagaaggaa aaaatggaca gttaccaaaa actgatccag
    17521 aacaatatcg ctataaacct aacagtgacc ttgaaaatga gaagcgaatt tcagatttat
    17581 caaaagtaaa tcaagaagac aaagcagaaa atgagggaca gttaccaaaa actgatccag
    17641 aacaatatcg gtatagatct gttaactccg aaaatgaaaa acgcatttca gatttaccaa
    17701 aacgaaatca agaagtacaa acggatgagg attccatcgt tctgccaaat gataatggaa
    17761 tcgaagttgg aactgaaaaa tcaacgaaaa ttggtttacc taaggcacaa aatttaccaa
    17821 tgaaagatag tcgtttgaat caattcaagg ataatgatca aagttttgtt catcaatcac
    17881 ctgcaggtac tgttcaaatg aaaccaagtc gtttgcctga aaatggaaat ctgacaactg
    17941 tttcttacaa tttatctgaa agtggtgaga cagggaatgc gtccgttcaa aatgttgtga
    18001 caagagaagc tgcaccaaaa cctattcaaa acgttcatac aggttacaag ccagcaaatg
    18061 tggcatttac tgcaccaaca aaaataaatg tgacaagaga aacaaaaaac aggaacaatg
    18121 cattgaaaga acaactattg agggatatca aacaggatat caaaccataa aaggcctaca
    18181 agttaggcct tttttctatg tatgaaaagg agaaatcatt tatggataaa tatagtaaca
    18241 tgaaaaaccc tccaagattt agtggagaat ggaagttttt cgcttttttc tttttagatc
    18301 tattaacgct tggtggctgc tttttttggg cgaaaatggt caataatctt gtccacttac
    18361 cactcttttt tgcactcttg aattatggct tatggattgc gatagcgtta gtgattgttt
    18421 ggcgacccta taataaccca ggtcaacgac aaatttttgc gattatcgag tctatttttt
    18481 ttgcggacaa gaaccattat catgcgttag atcctaaccg aaattatcat ggtacgtata
    18541 aggaggatga ataaatgcga gtcaaaggac aaaattcagt tgaaaggaaa tccattttgc
    18601 agtttggaaa accagaagag cgctctatct tggacttatt tccatataaa tcctgtacag
    18661 aagagggagt cttaattact agagaagatc attttcaacg ttattatcgt gtagtttcta
    18721 cagacgtgga aggattaaat gaacaagaga aactagaacg aatgaatcag ttgaccaccg
    18781 tcatgcgtac gtatgtgcca acaattaagt tagttagttt gaccacagag acggatctaa
    18841 cggaacaaat cgtgcaaaag cggcaattat tagataaaaa tcgtatggaa cagtccttag
    18901 gaagaaatct acaacgatta aaaaaatatg aaaagaaatt agtcgaagaa atccaggaat
    18961 tgaaacgagc agaacaagaa cgtcctgact taagcttctt tttcgtcatt gaaggaaaga
    19021 caatcaagga tttacaaacc agaaaccgtc agttaatgcg atctagtggc attttagggt
    19081 taaaaccact ctacaaaaaa gaattgatca agattctgta tcgaatgaac aatatgaatg
    19141 atgagtagga atggagaaga gagaatgaaa tatccgttga tatggttaga aaaagatgtt
    19201 ccgaaggaag agaccactag aaaatttcct cacactttaa gcctgggtaa gttgggaaaa
    19261 gggcaatctt ttaaccaagt aaagaatcaa acaagaagag gtgactgtac atgttagaag
    19321 caattaaaac agctttttca ccacaaaatg atcaaaaagt acttgatcca ttaaagcaac
    19381 taggaaatcg ggaaatagaa attgtaaaat ccaaagggta tgatttagat tttttaaaac
    19441 aagtgatgcc acaaggtggg ttaaattttg atgaagaata tgtgacgtat ggtaatggtt
    19501 attcacgatg tgtgacagtt tatcactaca caaaaaatcc tacgcttttt tggttggcca
    19561 atctcatgaa caatcctcat acagtggcca cactcgatgt tagcacagat gaaaagcaaa
    19621 aagtgattga tcaaatcaat cgtgccttag atgaattaaa tgatcgatca gataatgaac
    19681 gaaaagcgac tgaccgaaat aaaagcgcgg aagaatacct ggaactagcg gaatatgcta
    19741 gaaaattgac ttctaatggg gaaatttcta agcaagtcaa agtgagagta catgtttacg
    19801 gaaacacgat tcaagaagta gatgaacgag cgcaggaact gatcgaaaat ctcaaaggga
    19861 tggattatcg tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta
    19921 ccatgttcga tgaacaagct gaatggttcg gaaatatcaa agggcaatca ctaccatcgt
    19981 taaacattgg cggaggtttc ccgttccacc accaaacgct aaaagatcca ggagggacac
    20041 aatatgggga aaccatgaca ggcggcccat ttgtctggaa tgcgacgtat aaaaatagtg
    20101 tacggttgtc tactaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc
    20161 ttaaaatgat cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg
    20221 gaaaagactt tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat
    20281 ctgacggaat gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca
    20341 gtttatatga cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa
    20401 cacacttaga caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga
    20461 tgaaagcaga atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac
    20521 gcggctttac ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc
    20581 cagaagctta tccgacgttc aaagaatttt tagacttttt aggacaactg gaattaccag
    20641 gtgcttccca agagaaaaag aatcgaaaag aagaaatgga atccatcgta gaatccttat
    20701 gtgagacgta tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac
    20761 agttggtctt ttttgacatt gactctatta caggcttaga tcgaggggta caacaatgtc
    20821 aaatgtacat ggctatgacg ttgatttgga atcaggcatt gattcaagga agaagacaac
    20881 gacgtttaat taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga
    20941 ttgatgagtg ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag
    21001 tctaaaaact attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa
    21061 agggcttcca ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga
    21121 agctcggtga agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa
    21181 gttctctaga ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt
    21241 gagtatgtac aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta
    21301 gaaatgggag agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa
    21361 gtcgttgtta tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc
    21421 tagcatctaa gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg
    21481 agagtgcata ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta
    21541 gtgatggtga aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg
    21601 ctcgattcgt ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa
    21661 aaggtcacga gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata
    21721 ggaagttgcg gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca
    21781 aaagtttgaa gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca
    21841 ttgtcctagc atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg
    21901 gagtaacgat taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga
    21961 agcgattcgc acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg
    22021 gtaaattaag accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct
    22081 tacaagtatt agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc
    22141 caaatagatc agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca
    22201 tggaatatgt ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac
    22261 tcatgagaca aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga
    22321 aaatgttaag agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa
    22381 caccacaagg cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact
    22441 ggtggatagc aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga
    22501 aaaatggagc ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa
    22561 aagaaatata cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg
    22621 aagcgaaaaa agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa
    22681 tatctgaaga aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct
    22741 ttacgatgaa attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa
    22801 aagcacaaag catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc
    22861 ctaatagcaa agataccttt atctatattg gagtctataa cagtatggtt attgggattc
    22921 ataattactt caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac
    22981 tcagaccaag ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg
    23041 gaaagtacga aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt
    23101 ttccaatatt accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata
    23161 ttaataaata caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag
    23221 aatggaagat atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat
    23281 ttgcggacaa tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg
    23341 aggagcttat cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa
    23401 gtgatagcta tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta
    23461 cacaaagtga aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag
    23521 aaaaactcaa tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg
    23581 taggacggga aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg
    23641 cacgtacggt gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
    23701 tcgattaatc cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg
    23761 cgtaaagtag aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa
    23821 aatatgtctt ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag
    23881 atctttatgc gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca
    23941 atgacgaact cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat
    24001 tttggctcaa aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg
    24061 tttgatggag gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc
    24121 gtcgtgcaaa gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta
    24181 ttttgatcac tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt
    24241 cggattcaga ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
    24301 cgatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg
    24361 taggtgcagc agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg
    24421 aatcagcgtt tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt
    24481 ggtttgatgg ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg
    24541 gctggtacat gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag
    24601 ccacacaatc caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga
    24661 tcttaggacg aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag
    24721 gttgtatgtc aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac
    24781 attggggtga aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc
    24841 ctggcagcag tttagataaa agttccttca gcggtggtca attatttgga acgaatccag
    24901 gcggtgaatt ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc
    24961 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
    25021 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
    25081 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
    25141 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
    25201 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
    25261 ttttgaatag cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa
    25321 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
    25381 tgaaagagga acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg
    25441 agaagctcaa aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca
    25501 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
    25561 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
    25621 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
    25681 tcgacaaatt aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct
    25741 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
    25801 ttgtcgtatt tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa
    25861 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag
    25921 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa
    25981 cgtttcaaaa aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt
    26041 ttgattcttg ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc
    26101 acgaacgcct ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac
    26161 cagcaattag tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg
    26221 gctaatctta attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga
    26281 gccaaagtga ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa
    26341 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
    26401 caagaaccac aagatctgat tttttatatt cacgaagata aggtcaaaga tcacgtgaaa
    26461 gacgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa gaaagaacaa
    26521 aaagcagctc aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta
    26581 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
    26641 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa
    26701 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
    26761 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
    26821 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
    26881 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat
    26941 accttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
    27001 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
    27061 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
    27121 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
    27181 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
    27241 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
    27301 acaaataacc ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg
    27361 tgttaggtag gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga
    27421 tttgtgtgta gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt
    27481 gtgtagcaaa atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa
    27541 taaatttgga ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata
    27601 aaattgacaa acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc
    27661 aatacctaaa agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag
    27721 accaatttga tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt
    27781 tagaagaata tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat
    27841 agtattgtga atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt
    27901 atatctttac gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat
    27961 atctaattat agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga
    28021 aaaaataaat ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac
    28081 cgaaatggtt tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac
    28141 aaattcaact gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca
    28201 aaccaaaaaa tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt
    28261 agatccattt gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa
    28321 taaatcgcct gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc
    28381 aacatacatt gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc
    28441 cgaagaagaa caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga
    28501 aggagaaact ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc
    28561 tcaatcgttg atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa
    28621 atatctttct tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag
    28681 ggaacaaaaa gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca
    28741 taaaaaagct tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg
    28801 aagtgatctg aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat
    28861 ttatcaagat gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc
    28921 caaaacagat accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac
    28981 attattcgag aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa
    29041 cactaagcat atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta
    29101 tactgagatt gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac
    29161 attagacgca atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga
    29221 tcgaatgatc ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac
    29281 cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa
    29341 tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg
    29401 aaccaagctt tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac
    29461 aaaagaacct ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa
    29521 aagcctttat ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga
    29581 tttaaaaaag cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat
    29641 aaaaccatta atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg
    29701 aacactcaaa cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg
    29761 taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac
    29821 aattaatgca catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag
    29881 aatccaaatg gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa
    29941 tggaaagtca tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta
    30001 gaaaaagagc atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat
    30061 caatttttag ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt
    30121 gaaaaatatt ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg
    30181 gaaataaatg aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct
    30241 ctcacggaat gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact
    30301 ctttttcaat gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat
    30361 caaaagaaag tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg
    30421 gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta
    30481 gagcttttgt agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt
    30541 taaatctgac tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa
    30601 acgatatttt acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt
    30661 cggttgggaa agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt
    30721 caaaagctat ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat
    30781 ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa
    30841 gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa
    30901 atgggctata acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa
    30961 gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa
    31021 tatcaacgga agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa
    31081 aatatcctcc ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa
    31141 aatccaacca tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac
    31201 gggggatttg aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt
    31261 cgtgaattac ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat
    31321 ttctatcgag tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg
    31381 gagatcgtaa cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca
    31441 gaagcaggtt attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag
    31501 atccgaatgg atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca
    31561 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
    31621 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
    31681 acggcagaag agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
    31741 aaaaagcata cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
    31801 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
    31861 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
    31921 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
    31981 cgagaaacag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
    32041 atcaaaaaac cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
    32101 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
    32161 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
    32221 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
    32281 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
    32341 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
    32401 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
    32461 tttggaatgg acacttcgga gaaagctatt ccttatattg cagcatggac gaagaatggt
    32521 cagacgttgg atgataagga agcggcggaa cgtgggaaaa tcatgaatga tgtcagccgt
    32581 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
    32641 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagag
    32701 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
    32761 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
    32821 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
    32881 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
    32941 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
    33001 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
    33061 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
    33121 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
    33181 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
    33241 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
    33301 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
    33361 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
    33421 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
    33481 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
    33541 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
    33601 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
    33661 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
    33721 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
    33781 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
    33841 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
    33901 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
    33961 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
    34021 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
    34081 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
    34141 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
    34201 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
    34261 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
    34321 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
    34381 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
    34441 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
    34501 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
    34561 gtcacaagcg aaagcttatg ccgatagctt ttctaaagct acccgaaaag atgggtattt
    34621 tgagatccaa gatcgattgt ttacaggaga aactgtgatc acgtatggtt ttggtcattt
    34681 agtcgagtta gattcgcccg atatgtacga tgaaaaatgg aaacaatggg cattagaaca
    34741 tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga aaaagcagtt
    34801 tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga ctgatagcga
    34861 tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca atcaaggaaa
    34921 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
    34981 acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag cacgtcagat
    35041 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
    35101 aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct atatgattta
    35161 tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag gaaaagccca
    35221 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa cccaagcgac
    35281 acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa aacagccagg
    35341 tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc ttttttcttt
    35401 atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag atactttgga
    35461 agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg atacccctta
    35521 tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca agcatttttt
    35581 aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg tgaataataa
    35641 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
    35701 tgaacaacta agtccactgc agcaggctat ttatgaacaa gtgttaaaaa caacggtggc
    35761 catgtttgcg gaaaagtata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct
    35821 tcagttaaag gcaatcgaaa aggtgccgct agatctaggt tggaagaagt tatttggaaa
    35881 agagagcgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
    35941 aatggtgacg gtggatctac aagttcttga aaaagaaacc aagccaccac agccttatac
    36001 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
    36061 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
    36121 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
    36181 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
    36241 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
    36301 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
    36361 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
    36421 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
    36481 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
    36541 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
    36601 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
    36661 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
    36721 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
    36781 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
    36841 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
    36901 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
    36961 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
    37021 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
    37081 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
    37141 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
    37201 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
    37261 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
    37321 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
    37381 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
    37441 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
    37501 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
    37561 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
    37621 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
    37681 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
    37741 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
    37801 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
    37861 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
    37921 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
    37981 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
    38041 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
    38101 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
    38161 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
    38221 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
    38281 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
    38341 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
    38401 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
    38461 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
    38521 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
    38581 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
    38641 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
    38701 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
    38761 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
    38821 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
    38881 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
    38941 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
    39001 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
    39061 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
    39121 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
    39181 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
    39241 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
    39301 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
    39361 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
    39421 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
    39481 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
    39541 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
    39601 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
    39661 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
    39721 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
    39781 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
    39841 cgttatctct ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg
    39901 acaaggacaa gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattt
    39961 attctggcca acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag
    40021 aggaggcaga atcaaacatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat
    40081 tattcccaaa ctatcatgca ccacatactg ttgaatgcga ttgtggagaa agagcgaaat
    40141 gtatcaaaag ctatcgtttg tatcaatgtc caacatgcgg gaaaaaatat accttgtctt
    40201 atggcgatta cattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cacaacacaa
    40261 attattgact gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa
    40321 agtagcagaa gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga
    40381 tgcaccgact atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca
    40441 agaaaacatg gaaacttatg aaatcgaata ttggttaacc aaattagaga actaaaaagg
    40501 agaaaaaata tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg
    40561 gcacaaaaaa tggaggatat gacgttccta taccatgaaa cacgtgtacc caaaaaacat
    40621 tacaaggaaa aattgtcagt cgcagtggaa gaaatgattg aatcaggtgt cgaaatgaat
    40681 ctcattgcga cgtactatcg aacactggaa gaactgaaaa aacaaaatgg aaaatggttt
    40741 ttccaagcct tactatgctt ggaagtaggt gtcaaaccaa gcactatcaa accttccgaa
    40801 tatcaagcct tagaactgac ttatgcaaaa ttcgcacaga ttaagaaagc taaagcaatg
    40861 gctagcgact ggctagatac ttttgaaagt atcgaaaaaa atggtgcatt ttatacgatt
    40921 gaatcggagg agaaagataa tgaataaggt ggtttatcag ctaacaacag aagatgtttc
    40981 gcaattttta acaagcaaag aacacttgaa tgaagtaatc agacggattc aaaaagactt
    41041 tagttgtatg gaagaaatga agtactttat tcaaagagtg gaagaacagt tgtatatgga
    41101 aagcgaaaca agtcaaacga aggtcacagt cagccataat catttagacg tgtataaacg
    41161 tcgtcctgaa acggtaaaaa aaggaacaga gcttctacga ctagaaacag aaaaatacat
    41221 catgaatgtg tacctagaaa ggagtgagct ggtcgaaaac tttgtcagct acacgatttg
    41281 taggaaacac tattactcag aatcagaata ttattcaatt ttcgagagca tggaattaat
    41341 ggaagaagaa atggaagagc taaacagcaa aaatgcgacg gtagacgatt attaccttgt
    41401 atttactagg tttttgacgt gtgggacgga atctatcgtg ttaatgggaa tggattatga
    41461 actcgctgta cccgaagcat ttttgaggga atatgtagaa gaagtagata acccaggcga
    41521 cgactggtac acgactttaa ctgatttttt agacacctac acttacgacg atacagaaga
    41581 aatccaaaat gaacttgacc gtaaacgaat gccatatcaa cgatttgaaa ggaaaaaagg
    41641 taaataacca tttataccga aaagtgagga tgtgtgaaat aagaacaaaa aaccttgaaa
    41701 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
    41761 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
    41821 atcattcctc tattgttcgt actgtttgtt ggcgagatac ttatgcgtca tgctgcagct
    41881 gtacatttaa acttcgtagt attcttagga atactggtat tttgtttgat cttttggcga
    41941 tttgtattag cccttgtgag tctactattc aagatgattg cagccatatt tttcataggg
    42001 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
    42061 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
    42121 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
    42181 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
    42241 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
    42301 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
    42361 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
    42421 atataaatgt taacatagtt tatggattca tggaatgtta atctaattta atagaaaagg
    42481 ataattaagt atatacgttt tttcttatgt atgttatact atgtatatac agaaagaggt
    42541 gtctactatg gaaatgttac ctataaaaaa atggggaaat agtaatggcc ttagattgcc
    42601 aaagtatatt atggaatatc ttggaattca tacagaagac aaagtgaaga taatacaaga
    42661 agaagtaaac ggtcagaagc ggctaattat cgaagcggct aatatagaaa atgaattaac
    42721 aattgaacag ttatttgaaa attacactga tgaaagaaat catgtgacga ttcaaaattt
    42781 aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta gacaaggcga tattctatta
    42841 ttgaatacag cacctcgatc tggacacgaa cagacaggta aaagacctta tattgtttta
    42901 agtcatgata ttattgcgga ttatagtaac gttgttacag ttgctccaat ttctagtaca
    42961 actagaaact atccgctata tgtaaatata aactcagaat atgaaatgaa gacaacagga
    43021 aaagtattat tagatcaact aacgacaatt gattatgaag ccagagagtg tatttttttg
    43081 gaaaaagcgc atgatagttt agttgaggag ttattgatga aagttagaac agtttttcaa
    43141 aaagtaagca aataaaatag aaacagcagc atgatcaact attcgtgatt gaccatgctg
    43201 tcttttttta ttctatatac ggtttgtctc gtaacattgt agtcttttgc aatcttagcg
    43261 atcgcaacac ctttttttag atcctcaacg atgtttttat aaacaagacg acgttgggga
    43321 tcttttgcat tggcactgta tagcttagga cgtcctttat aaacaccgtt cgcttttgca
    43381 atcttaatgc cttgtgcctg acgtcgttta gattcggtac gttcttgttc agcgatcatg
    43441 gccaaaattt gaatgatcaa atctttaatg aatttatcca ggagaggatt gccaattgct
    43501 tcggccatga tcggtagact tgtgatgatc agctgcacat ttttcttctt caagtaattg
    43561 actgaagcga tgatctcgtc gtaatttctt ccaagtcgat caatagcttc cacaataaaa
    43621 atatcttgat ctcggacgaa atccagtgct tcttgaaaaa caagtcgatg agtaatcgtt
    43681 gcgccagatt gtttctcaat gaaaattctc tctgcaccga attttttcat ttcttcaatc
    43741 tgccgttgtt cattttgatc gatggatgag acacgtatgt atgcaactct caaaattttt
    43801 acctactttc tgcaactatt taaaaaacac cctaatcata cacgagaaaa ccagattttt
    43861 caatgtcgcg gtcgtgtcta gttagggtat accttatata gacaaaaact aatgatggag
    43921 gaaatagtat gtgtcacttt tctttttaaa tggcaatatt ttttgtaaat aaatgtcaat
    43981 caagaaaatt taaacgatta tgacattaaa aatatatttt atgaattttc tagtatataa
    44041 cttgatttat ctgttaagat ttattaagaa aacgttttct tatttttgat ttcgaggtga
    44101 tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg aattagtgta
    44161 actggaacta caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat
    44221 aataaccaag aattaactcc tttagatagg aacggaatag ccattggtaa cccaactaac
    44281 gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctcg agcagcttct
    44341 aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct
    44401 cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat
    44461 ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact
    44521 agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc
    44581 gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact
    44641 ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa
    44701 agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt
    44761 gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac
    44821 actttctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata
    44881 acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa
    44941 ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat
    45001 ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt
    45061 ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat
    45121 acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc
    45181 gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg
    45241 gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt
    45301 gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga
    45361 cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta
    45421 gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc
    45481 atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt
    45541 ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa
    45601 ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg
    45661 ttaatattaa
//
LOCUS       DAZKOK010000010        45496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000010 DAZKOK010000000
VERSION     DAZKOK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.010"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..1730)
                     /locus_tag="ACG90W_000510"
                     /pseudo
     CDS             complement(95..1730)
                     /locus_tag="ACG90W_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2053..2532)
                     /gene="rlmH"
                     /locus_tag="ACG90W_000511"
     CDS             complement(2053..2532)
                     /gene="rlmH"
                     /locus_tag="ACG90W_000511"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0476903.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2960..4249
                     /locus_tag="ACG90W_000512"
     CDS             2960..4249
                     /locus_tag="ACG90W_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0476904.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4288..4893)
                     /gene="ytpR"
                     /locus_tag="ACG90W_000513"
     CDS             complement(4288..4893)
                     /gene="ytpR"
                     /locus_tag="ACG90W_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0476905.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5098..5562)
                     /locus_tag="ACG90W_000514"
     CDS             complement(5098..5562)
                     /locus_tag="ACG90W_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0476906.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5584..5904)
                     /locus_tag="ACG90W_000515"
     CDS             complement(5584..5904)
                     /locus_tag="ACG90W_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0476907.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6048..7127)
                     /gene="pepA"
                     /locus_tag="ACG90W_000516"
     CDS             complement(6048..7127)
                     /gene="pepA"
                     /locus_tag="ACG90W_000516"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0476908.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7327..7677
                     /locus_tag="ACG90W_000517"
     CDS             7327..7677
                     /locus_tag="ACG90W_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0476909.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7900..8847
                     /locus_tag="ACG90W_000518"
     CDS             7900..8847
                     /locus_tag="ACG90W_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0476910.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8904..10322)
                     /gene="pepV"
                     /locus_tag="ACG90W_000519"
     CDS             complement(8904..10322)
                     /gene="pepV"
                     /locus_tag="ACG90W_000519"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0476911.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10471..11316
                     /locus_tag="ACG90W_000520"
     CDS             10471..11316
                     /locus_tag="ACG90W_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0476912.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11320..11760
                     /locus_tag="ACG90W_000521"
     CDS             11320..11760
                     /locus_tag="ACG90W_000521"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0476913.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11810..12004
                     /locus_tag="ACG90W_000522"
     CDS             11810..12004
                     /locus_tag="ACG90W_000522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476914.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12140..12826
                     /locus_tag="ACG90W_000523"
     CDS             12140..12826
                     /locus_tag="ACG90W_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0476915.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13234..13923)
                     /locus_tag="ACG90W_000524"
     CDS             complement(13234..13923)
                     /locus_tag="ACG90W_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476916.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13898..16027)
                     /locus_tag="ACG90W_000525"
     CDS             complement(13898..16027)
                     /locus_tag="ACG90W_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0476917.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16075..17412)
                     /locus_tag="ACG90W_000526"
     CDS             complement(16075..17412)
                     /locus_tag="ACG90W_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0476918.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17664..18023)
                     /gene="rplT"
                     /locus_tag="ACG90W_000527"
     CDS             complement(17664..18023)
                     /gene="rplT"
                     /locus_tag="ACG90W_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0476919.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18076..18276)
                     /gene="rpmI"
                     /locus_tag="ACG90W_000528"
     CDS             complement(18076..18276)
                     /gene="rpmI"
                     /locus_tag="ACG90W_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0476920.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18348..18872)
                     /gene="infC"
                     /locus_tag="ACG90W_000529"
     CDS             complement(18348..18872)
                     /gene="infC"
                     /locus_tag="ACG90W_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0476921.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18916..19041)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19192..19824
                     /locus_tag="ACG90W_000530"
     CDS             19192..19824
                     /locus_tag="ACG90W_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0476922.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19826..20731)
                     /locus_tag="ACG90W_000531"
     CDS             complement(19826..20731)
                     /locus_tag="ACG90W_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0476923.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20816..21475)
                     /locus_tag="ACG90W_000532"
     CDS             complement(20816..21475)
                     /locus_tag="ACG90W_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0476924.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21791..22735
                     /gene="mvk"
                     /locus_tag="ACG90W_000533"
     CDS             21791..22735
                     /gene="mvk"
                     /locus_tag="ACG90W_000533"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0476925.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22728..23705
                     /gene="mvaD"
                     /locus_tag="ACG90W_000534"
     CDS             22728..23705
                     /gene="mvaD"
                     /locus_tag="ACG90W_000534"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0476926.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23718..24803
                     /locus_tag="ACG90W_000535"
     CDS             23718..24803
                     /locus_tag="ACG90W_000535"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0476927.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24800..25864
                     /gene="fni"
                     /locus_tag="ACG90W_000536"
     CDS             24800..25864
                     /gene="fni"
                     /locus_tag="ACG90W_000536"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0476928.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26113..26916)
                     /locus_tag="ACG90W_000537"
     CDS             complement(26113..26916)
                     /locus_tag="ACG90W_000537"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0476929.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26936..27778)
                     /gene="kduI"
                     /locus_tag="ACG90W_000538"
     CDS             complement(26936..27778)
                     /gene="kduI"
                     /locus_tag="ACG90W_000538"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0476930.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27794..28801)
                     /locus_tag="ACG90W_000539"
     CDS             complement(27794..28801)
                     /locus_tag="ACG90W_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0476931.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28808..29452)
                     /locus_tag="ACG90W_000540"
     CDS             complement(28808..29452)
                     /locus_tag="ACG90W_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0476932.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29552..30310)
                     /locus_tag="ACG90W_000541"
     CDS             complement(29552..30310)
                     /locus_tag="ACG90W_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0476933.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30437..31588)
                     /gene="fucO"
                     /locus_tag="ACG90W_000542"
     CDS             complement(30437..31588)
                     /gene="fucO"
                     /locus_tag="ACG90W_000542"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0476934.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31670..31981)
                     /gene="rhaM"
                     /locus_tag="ACG90W_000543"
     CDS             complement(31670..31981)
                     /gene="rhaM"
                     /locus_tag="ACG90W_000543"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0476935.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31983..32828)
                     /gene="rhaD"
                     /locus_tag="ACG90W_000544"
     CDS             complement(31983..32828)
                     /gene="rhaD"
                     /locus_tag="ACG90W_000544"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0476936.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32842..34098)
                     /gene="rhaA"
                     /locus_tag="ACG90W_000545"
     CDS             complement(32842..34098)
                     /gene="rhaA"
                     /locus_tag="ACG90W_000545"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0476937.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34095..35555)
                     /gene="rhaB"
                     /locus_tag="ACG90W_000546"
     CDS             complement(34095..35555)
                     /gene="rhaB"
                     /locus_tag="ACG90W_000546"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0476938.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35708..36577
                     /locus_tag="ACG90W_000547"
     CDS             35708..36577
                     /locus_tag="ACG90W_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0476939.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36703..37602)
                     /locus_tag="ACG90W_000548"
     CDS             complement(36703..37602)
                     /locus_tag="ACG90W_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0476940.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37767..38591)
                     /locus_tag="ACG90W_000549"
     CDS             complement(37767..38591)
                     /locus_tag="ACG90W_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0476941.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38602..39897)
                     /locus_tag="ACG90W_000550"
     CDS             complement(38602..39897)
                     /locus_tag="ACG90W_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0476942.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39913..40392)
                     /locus_tag="ACG90W_000551"
     CDS             complement(39913..40392)
                     /locus_tag="ACG90W_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0476943.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40398..41426)
                     /locus_tag="ACG90W_000552"
     CDS             complement(40398..41426)
                     /locus_tag="ACG90W_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0476944.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41443..42549)
                     /locus_tag="ACG90W_000553"
     CDS             complement(41443..42549)
                     /locus_tag="ACG90W_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0476945.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42778..45375)
                     /gene="adhE"
                     /locus_tag="ACG90W_000554"
                     /gene_synonym="adhC"
     CDS             complement(42778..45375)
                     /gene="adhE"
                     /locus_tag="ACG90W_000554"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0476946.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13172 a         9671 c         8252 g        14401 t
ORIGIN      
        1 agattaagct tcattattat aatataaagt ttaaatttgg tttattactt actttttctt
       61 attctctagt aagctaatat tgaaaaaaat gtaatcagat cattattttt atatgttgta
      121 acttcctgat aagaaaacta attaaaagaa agaggtaact acataaacta attaaaaaag
      181 ctaacttcat agttggagga taaatggaag aaacttcaat gattgtttct ccttgtgttc
      241 cttcaatcga cactagtcct attatcaatc tctctttttt gtccatttac ttttgcgact
      301 gttccccaat aatcaaatac aggaagcgtt attttttgtc ctttagattc atgtactgta
      361 tagactattt tcgaatccat cacttgataa ggcatagtat gccattcctt gccaattctt
      421 acttcatgat tctgcaaatg ttctttgtat tgtaatgctt ccgtgggtgc atagtcataa
      481 agtcctccgg gaaggagtgt cgtgtaacga ataagatttt cttgattcat taccaagtga
      541 tctctctgag tgacaacttt atgttcatag tttgcttttg ttgcagcaga tacagccaac
      601 agtaataaaa caattgccgt tatattaact acttttcttt gcttagctga tgtgctcttc
      661 atgaaaacga acgataaaat caacgagcca aacagtactt gaaagcctaa gattcgccaa
      721 ggaaattgaa tcatttgaat gggtgtattt tgtaataaag accatggaaa aaatttggtg
      781 gataagagcc aagagactac cgcactaagg taaatcactt tttctttgcg attaaatcct
      841 ttaaaaaaga agagtggaca gatcaaagaa ataataccaa tgattcctac cgtaaaggtt
      901 gtcaggtcat tattcatcgc accaatcaaa ctatccgaga catttaatgc ttggtgttgt
      961 aaggtcgttt tatctggctc attaatcgat tgaaccatga tttgtcggaa catcggatac
     1021 caaaagtatg ctgttaaaag aatcgtcatt cctactgcct ttgacaactc cacaatcaac
     1081 cggatgcttt ctttttttct tgtttcactt actacaaagc cgcctaagac tagtaagcca
     1141 acggctatta cactcataaa agtggaaaga acatgtgtat aaatgagtaa gctcatactg
     1201 actgctaatg gaattgcaca tggctttttc tcataaatca gtgcgtaagc ataatacata
     1261 actatcggta taaccgtaag tgcaatcgct tcagcaatag cagaacgatg ataaatatca
     1321 atcgtacggt acccataaaa tgtatataaa cagctaaata taaaagccgc atcgattctt
     1381 tttaaaaatt ttagtccata atgaaacatc agcatgatag tacaaacatt caatagccaa
     1441 acatataaga tataactaac gattaaatta ttacttatcc aataaaaaat gactgccgga
     1501 aaaaaggtca agtagggata aaaataattc actccgctcc cataatgatt aaacgtcgta
     1561 aaattgattg gaccagaaaa aattgaagat aaccctttga ctctagcaaa atggaaggca
     1621 atatcccccg atgtagaaat agtatgaaca tttctaaaat aattgattga ataaagtcca
     1681 gcaataattg ctaggagaat ggacaatatt atttttttct ttacttgcat tacttaatta
     1741 tatactcctt tcatggattg ctttatctag tataacttaa attttaaata aatcgttatt
     1801 tatttttact ctcacttaaa aaagcactaa aattataaga gagggaggta atggtctgaa
     1861 caccaagaat tgttccaaca tcgaattgct taactaatga gttcaaagct taattatgtg
     1921 gctcttatat ttaccgagcg tggattgcga tctctttagc gaaacaaaca tctttcataa
     1981 aaaaaacagc catatccgct ttcttggata tgactattta ctactacttt actatctttc
     2041 attcttcttg tctcacttat gataaggcgc ccccgcattg atccggaagg cacggtagat
     2101 ttgctcgaca aggatcagac gcatcaattg atgtggatac gtcatttttc caaaagagat
     2161 ctgagcattg cttcttttca tgacggcttc gctgagtcct aaagagccgc cgataacaaa
     2221 aacaaggtgg ctttttcctt ggatacctaa ttggtcgatt tgtttagcaa aagattcact
     2281 tgtaggattt ttcccttcaa ttgctaaggc atagacgtat tcttgttctt tgattttcgc
     2341 taagatccgt tccccttctt tttcttttac ttgtctcatt tgcgcttcgc ttaaattctc
     2401 cggggctttt tcgtcaggaa cttcgatcaa ttcgattttt gcataggcat tcagccgttt
     2461 gacatactca ttgatacctt ggatcaggta tttttctttt aattttccta ctgagataat
     2521 tttgatattc atgttttctc tcctttggtc ttcatgctca ctttgtttat tataatcaat
     2581 tttttcgtct atatgtttat tttggctatt tttgttaaaa agatttcccc aacagttatg
     2641 cacaacatcc acaggtttat ccacagaaaa cagtgttatt ttaccttatt tcatcacatt
     2701 cattaggcaa acatacgttt tataagaatt tatccacttt atacacaggc tgtggatatg
     2761 ttttatccct tatttttcca atcctatccg cgtagtttta cacagtatct gtggaaaaag
     2821 aaaatcaaat attttcatct gaaaacaatg atttttccta aagtttttca taaaaagttc
     2881 acagtctcaa agtagactaa agtcaacaaa taagactata ctaacagtag ataaatgaat
     2941 gtaattggag gaggaacaca tggatagaaa aaatgtgaca ccaaagatga aaaaaagtaa
     3001 aaataatagc ctctggcgta agttaggtct ggggcttgtt ggagggatcg ttggaggact
     3061 actgacagct ggcatttttt atgcagcgat gggcactggc aacaatagtt ctactgctac
     3121 aagcggcaat cagaattctg ccggtgaaac agttgtggaa aatgtcaaag tcaatgtaga
     3181 ttctgatatc accaaagcgg tagataaagt tcaagacgct gtcgtttctg ttatcaactt
     3241 acaaagtcaa aaccaatcaa gcggttttgg cggactattc gggcagcaag aagaaagtag
     3301 cagctcagat gatagtaatt tggaagctta tagtgaagga agcggcgtga tctacaaaaa
     3361 agacgggaat actgcttatg tagtcacgaa taatcacgta gtagatggcc agcaaggtct
     3421 tgaagtcttg atgaaagacg gtacgaaggt aaaagctgaa ttagttggaa cagatgctta
     3481 ttcagattta gcggtgttga agatcaattc agacaaagtc gaaacagttg cctcttttgg
     3541 tgactcaagt gctctgaagg ttggtgagcc tgcgattgcg attggttccc cactaggatc
     3601 tgaatatgca aattctgtca cttcaggaat catttcttcc ttgaaccgac aagtgacaag
     3661 tacgaatgaa tcgaacgaaa cagtcaatat caatgcgatc caaacagatg cagcaatcaa
     3721 ccctggaaat tctggtggtc ctttagttaa tatcgaagga caagttatcg gtatcaactc
     3781 aagtaagatc gcaagtactt ctgaatctac ttctaatgta agcgtagaag gaatgggatt
     3841 tgctattcca agtaacgatg tagtgaatat catcaatcaa ctggaaaaag acggaaaagt
     3901 gacacgtcca gctctaggta tcacaatggt cgacttatct gccgtttcta ctcagcaaca
     3961 agaacaaatc ttgaagattc cttcatctgt aactaacggt gtcatcgtga caaatgtcca
     4021 aacagctaca ccggctgaaa aagctggatt gaaacaatac gacgtcatta ctaagatcga
     4081 tgacaaagaa gtttcttctg gtgtagaact tcaatctgct ttgtataaga aaaaagtggg
     4141 agattctgtc aaaatcactt actatgaagg ctcgaaagaa aaaacaacta ctgttgaatt
     4201 gacgatcgat caatcggcat taaaacaaaa tcaaaatagt gaaaattaat aaaaaagagg
     4261 ttgtgacaaa gacttgtcac aacctcttta ttttggaaat gcttctccga ctacggcatc
     4321 ctcaggcaat actaagattc ctttttttgc tggagcattt ggcaaatgca attcacgagc
     4381 ggaacagatc attccatagc tatctacccc tcgcaaagtt cctggccaga tcatcattcc
     4441 atctggcatc atagctcctg gttttgcaac gacaactttt tgtccagctt cgatattggg
     4501 tgcaccgcaa acgatttgca atactgtacc attgtctact tctgtctcag taatcgataa
     4561 atggtcagaa tcaggatgtg ggacgcatgt ttttacgtac ccgacaacga tcttcggttc
     4621 atgatcagct tcaagctgtt cttcgaatcc tgcttctttg atcaatcgat tcaatttcgc
     4681 taattcctca tctgttaagc ttacttggcc gttcccttcg attgttagat gatcggagat
     4741 atggaaaaag ttccaagcaa caacttgacc ttgttcatct ttgatccgag caacattcca
     4801 tttgcgttca acagtgtttt ctgttccttt atcgtcggct ataataacca tcaaggtgtc
     4861 tccgacgtgt gctttgttat aagcaaaaat catgttcttt cctccattat cctttgctga
     4921 gatttatctt aacaaaaaat tgcattcact gcatgaaatt tttttcatgc tttatttccg
     4981 aagaagcctc ttctggaaca ccatttattc gtaacaaggg actgtgacaa gacttttgtc
     5041 acagtccctt tactgtttta gttaattgat taaaaaggtc gtacaagttc cacttgctta
     5101 attacttggc tgttcttctt cgtcagaaac gatcaagaca tcacaaggtg aatgacgtat
     5161 cacgtagctg gctacacttc ctgtcataaa gcgttcgact gcatttagac ctgactgccc
     5221 aaccatgatt agatcgatct catatttttt tggcaatttg actgccatgg attcacgaac
     5281 agaaccatat gcaataattc cttctatatt tgtgtaatca acagatttcc catattcttt
     5341 acattcttcg atcatttcct tggctgtttc agtttgctga tcgactagat tttgatcaat
     5401 aggtgcatag cccataaagt tataaaattg ctgatcgatt acgtttgcta catatacttt
     5461 cccattatta cggcgagcca cttctaccgc ttttttgaaa gcagcaagtg cttgttcact
     5521 accgtcgata cctactaaaa tattcttata ttcttgattc atcttgaccc ctcctaacag
     5581 atgtcagcct tttgttacac tttctaggaa cgattcgatt tcctgttttg ttttacgatc
     5641 tttattcact agacgaccta attcttttcc gtcttggatc acgacaaaac ttgggatacc
     5701 aaaaatgttc cattcagcgg ctacatctat gtacttgtca cggtctactt caataaattg
     5761 ccaatcagaa aagtcatttt caatttcagg catttgcggt ttgataaaac ggcagtctcc
     5821 gcaccagccg gcagtaaaga aaaatacact ctttccagtt tctacgtagc ctgctaattc
     5881 ttctaaacta tttgggataa tcattctttg tcctctactt tctgtttatg tatttttcaa
     5941 ttaaattata cacctttctt tcgaactaac aaacattgat cctcttttta taaaaaaaat
     6001 ctggcaaaaa gcaatctttt gccagatttt atagtttcac tatcgcatta ttttccgtat
     6061 acgatcgtat ttactgttga tttatccaaa cctttcagtg tttgaatcaa catttctcgt
     6121 gcagcttcaa aatcgcgaat actgaacatc gtttgatgcg tatggatata acgtcccact
     6181 acgccaatca ctgtacttgg gattccttcg ttttgtgtat gagcagcacc tgcatctgta
     6241 ccgccttttg aaacaaagta ttgataaggg atattgtttg tttcagcgat atccagcaag
     6301 tactcgcgca aacgaggaag catgatcaaa cctggatctt ggatacgcat caatgtacct
     6361 tctcctaaat gtccgaatgt gccatttttc gtcactgtat catctgctgc agagcagtct
     6421 actgcaaaga acagatctgg cttgaattta ttgacagaag ctttagaacc acgcaagcct
     6481 acttcttcct ggacattcgc gcctgcaatc aatgtatgac ctaatgtttc attttgcaac
     6541 gcttctaaag cttctaatac cattgtacag ccatagcggt tgtcccatga tttactgata
     6601 atattctttc cgttggctgt tttgatcgtt tcagcgtaag gaacgattgt gtctcctgga
     6661 aggacaccgt aactcattgc ctcttctctt gattcaaacc cagcatcgaa caaaatatcg
     6721 gttacttcta ctgatttttg tccgcttgtt cctcgcaata aatgtggggg aacggaagaa
     6781 gaaatacatg gataattctt accagtagag gttttaagtg taaaacgctg agcagaaaca
     6841 acgtaaggat tccagccgcc tagaggtacg actttaaaaa gcccgttttc ttggatttgt
     6901 gtcagcatga atccgacttc atccatatgt gctgcgacca ttacacgggg tgcatccgct
     6961 tcttcatgat ggcgcagacc aaaaatgccg cccaaaccat ctaactgtaa ctcatctacc
     7021 agcggttcca tatgactttc catataagcg cgaacatctt gttcaaaacc gcttgttcct
     7081 tgtaattctg ttaactcttt gattcgttga aatgtttttt cttccatatg ctattcgtct
     7141 aaatttcaga ctaaactcct ctcagactta tattgatctg cggataaaat atgatttttt
     7201 ctgcagaccg atcgtattta ttatagctta ttttttctgg tagcggttat ttatttcaaa
     7261 gaaaatatga gagtgtggta aaataagcac aaggagtaac aagtgagaag aaggaggtat
     7321 gtccacatgt atgaagaaag cgaactaagc tattttaaag gcgggttagc agttggaatc
     7381 ggtctaggat tattgagcgg ggtagcttca acgatttggt atcatcgcaa aaaagtattg
     7441 tcagcagacc aagtacttga aaatatcaag tcatcttttc taaaagaagg tgcaatcgaa
     7501 ggttcttgga tctcttttga aaaggaacct attaggaaat tcgccgttcg gtcgagtgct
     7561 tatcggggag gtctttctcg aatagaagat ggcgaaactg tttattatga atttttagca
     7621 gatgctcata caggaaccgt attagaaatt tcaagaaaaa aaggtgcaac agattaatac
     7681 gtcaaaaaag tgagagaacg aatacgcacc cactattcaa aagagaggta tcatgtcctt
     7741 cttttgtagt gggtggtttt ttagatagag ctttccaaac atatattcca gtattttctt
     7801 tttaaataaa tgatatttag cgggaaaaac tagcatttcc tttgattatg aggtattttt
     7861 aatttagctt ttgttttcta tagttaagga gagtgaaaaa tggaacaaca agaaaaaatt
     7921 gctgttctga ttccttgtta caatgaggaa gcaacaattt caacagtcat tgcagacttt
     7981 aaacgagaat taccagaagc agatatttat gtctatgata ataattcgac cgatcgaaca
     8041 tacgaattgg ccgttcaagg tggagcaatc gtaaaaaaag aacctcgtca aggaaaagga
     8101 aatgtgatcc gccaaatgtt ctttgatatc gatgccgact attatttaat ggtagatggc
     8161 gatgatactt atcctgcaga agctgtacat ggtttgctcg agaaattacg ttctggtgaa
     8221 gcagatatgg tcattggtga tcgcctatca aatggaacct actttgatga gaataagaga
     8281 cctttccacg attttggaaa taaccttgtt agaaatacaa ttactagaat gtataaaaca
     8341 aaaattcttg atgtcatgac cggctatcga ggattcaatc gtatctttgt caaaagtttt
     8401 cctattatga gttctggttt tcaaatcgaa acggaattga cgatccatgc tttagataaa
     8461 aaattcaagt tcgttgaaat cccaatcgat tatcgtgatc gtcctgaagg cagcgaatca
     8521 aaattgaata cattctctga cggattcaaa gtgatcatga tgattattaa aatgtttaaa
     8581 gattataagc ctttgctttt ctttgggatt tggaccatct tcttcttttt atttggattg
     8641 gtcacgggga ttcctgtgat tcgcgaatat atgctgacaa gctttattac acgcatccct
     8701 tctgcgattt taagtactgg attgatgact ttagcacttc tttcattagt aacagggttg
     8761 attttggata cagtcgtcac gaacgctaag aaagaatatg aattgaatct atatcatgtt
     8821 tatcatgaat accacagtag aaagtaaaaa aaggatcaag actgcaaaaa taatttgcaa
     8881 ttcttgatcc tctttttaat taattatttc gccaaacgat agatggcatc tgcatagatt
     8941 gctgttgctc tgaacaagtc atcaagactc ataaattcgt ttgcttgatg cattgtatca
     9001 gtatatcctg ggaacatcgc tccataagca acgccacgtt tcaacaaacg tccgtaagtt
     9061 ccgccaccga tgatttgttc gtagcctttt tctccagtgt ggtcttcata gacttgcaat
     9121 aatgtagcca ccaatggatc atccattggt acgtaatgtg gttccattac gcgagcacct
     9181 cgagtaactg ttgcgccttc ttgccccact gtttgttcga tgtcactttg caatccgtct
     9241 actgttacac cttttggaaa acggaaattc atattgatga agttcgcttc ctctgtacca
     9301 ttttcttcaa aagcaaatag tccagcattc attgtcaatt tgcccatttt ttcatcttca
     9361 taagcgactc ctagtttttc tccataaaaa tcttcatgga cataagcagc tgctacatgg
     9421 ataaattgtt tagcagatcc taggaattca tagttatcta agaacaatgc caagaaagaa
     9481 cctgcattga taccagattg tgggcttgca ccatgtgcgc ctttaccaac taattcgatt
     9541 tttacatacg tattgtccgc ttcaatcgtt ccgcttactg gatttgcttc gatgaattgg
     9601 taaaatgctt cttccatcgc gatagctgca tctgcacttg gtacttgaag tgctgcagtt
     9661 gctgttcctg gcaccatatt ttcgcgaagt ccggaaacaa aagatttcaa gacataatct
     9721 cctgcattgt cgcctttgaa gcgtaaagct aaagaaacat ttcctttttc accattgatg
     9781 attgggaatt cagcatctgg tgagaaacca aaatctggtt cttcttcatg ttcgaaataa
     9841 taagccatat cgccccagcc gctttcttca tcgctgccga ctacgaagcg aacttttttt
     9901 gatagtttca agtcaagttc tttaatgatc ttcatcgcat agtaagctgc catacttgga
     9961 cctttgtcat cgcttgatcc gcgtgcaaag atctttccat ccttgattac tggttcataa
    10021 ggatctgtgt cccagccatc tcctgcagga acgacatcca tgtgtccaaa tatccctagc
    10081 gtttcgtctc cttcgccaag atcaatatgc cctgcatagt tatcgacatt ttttacttta
    10141 aatccatcac gttcgccata agctaacata tgtttcaatg cgtcgcgagg tccaggtcca
    10201 aatggtgcat ctggtgttac ttttgagtca tcgcgctcgc tgttgacacg taaaagattt
    10261 ttcaagtctt caagcaagtc ttctttacgt gcttctactt ctttttgcca atcaattgtc
    10321 ataaagttaa tcctccactt atttaagtct tcgtgttcat gataccacaa attacagaaa
    10381 aaaatagttc aaaaaatctg acaattcctt ttcttatctg tttttctcta aaaaaaatat
    10441 acgctataat gagtaaaagg aggtttttag atgctgataa acgaacaact agtgaaaaga
    10501 gtcatcacac ctcgtccaaa aatttcacat aaaggaacat ttggacgtat cgttttgatt
    10561 ggcggcaatg aacaatatgg cggagcaatc attatgagtg ctttagctgc cgtacacagc
    10621 ggagcgggtc tcacgactgt agtaactgat gaaaaaaatc ggagtgctct tcacagtcat
    10681 ctgccagaag caatgattgt agtttggacg gatactgacc agtttctttc cgtaataaag
    10741 caagcagatg tactcttgat tggtcccgga ttgggtactt cttctaagag tttggaacgt
    10801 ttcaaacaag tactagaaaa ccaatcaaaa gaacaatggc tggtgattga tggctctgct
    10861 ttaagtttac ttgctgctaa tcatcttttg ctaccctatc ccgaacaaac tgtacttaca
    10921 cctcatcaaa tggagtggca acggttgagt tctctaccga tacaagaaca acgggataaa
    10981 gaaaacaaaa agaagcaaca ggaaatcggt gctgtgctcg ttttgaaaag tcatgagaca
    11041 aaaatttatg gtcaccagca cttctacacc aatcctttag gttctgctgc tatggcaacg
    11101 ggcggcatgg gtgacacttt agctgggatg atcacaggat ttttagccca gtttcctcaa
    11161 aatgaccaaa cgattgcagc agctatttat cttcatagtt tcattggaga ccagctggca
    11221 aaagaacaat atattgtttt gccgacacaa atcagcgaag cacttccgaa atggatgaat
    11281 tatttttgta aaaaatcaat ggaaaacgag gcatgagtca tggagaaatt tacaggttat
    11341 gtgcgagaac ctttttatta tgaaacagat caaatgggga ttattcatca cagcaattat
    11401 atccgctggt ttgaagaggc tcgggtagcc ttattggtac atctagggtt tccctataaa
    11461 aaaatcgaag aatctgggat catcatcccc gttctggaag taaattgcag ctataaagaa
    11521 atgatccatt acggagaaaa agtgagcatc catccaacta tccaaaagta tacggggaca
    11581 cggttggatt ttagctacga aattgtggga actgaagaca aaaaattaaa aactactggc
    11641 agtagtaaac actgtttttt actttctgat tcgcaacgat tagtgaagct gagcaaagtc
    11701 aatccggagt tagatcagtt attccaagcg tacgcttctt tggatgatgt aaattcttaa
    11761 taaagaccgt ctaagcattt ttcttgtatt gtgtcgagta gttcgtataa tgaaaagaaa
    11821 aaagcaggtg atccccatgt atgtcgttaa agtaatgcac ggttacatcg ataaaactgg
    11881 ctgtcgcact cgggaaaaaa acttagacaa tctgctcatt ttcaaagaca aaaaagaatc
    11941 cgaagctttt gctaaaagaa tcggcggtcg tgtcaaaccg atccaagagg tacgaccaga
    12001 ttgattcaat atttttcttt tcgaggctct aataggcatg cctatcaggg ctttttttgc
    12061 agactgtgtt tttatgccca cacgatttgt aagcgattta tgttataata gattatatct
    12121 gcatagaaag gaaggttaaa tggttatcaa taatttgcaa cagcaagctt atgaatctat
    12181 acgtaaacaa atcatttatt ccgagttagc acctggaagt aaaatctccg ataaagtgtt
    12241 agaagaacaa ctagctatcg ggcgtacacc gattcaagaa gcactgatcc aactgcgcaa
    12301 tcaagagctt gtaaaaacca ttccacagtc cggcacttat gtttcaaaaa tcgatatccg
    12361 atctgcttca ttagcccgtt atacgcggga aaagttggaa aaaccgatcc tgcaagaatg
    12421 cagtgcgaaa atgaccaagc aagatcaagc taaactagaa aaaatactag aacaaaccga
    12481 tcaggcagta atcgctcaaa ataaaaaagc tttttttgat ttggatactg attttcaccg
    12541 aacttgttat gaaattgctg gaaaacgaga aatttgggat tggctagaaa gttatagtac
    12601 gcacttgaac cgtttcagat ggttacgttt gacgatttca gaattagact ggggtcgagt
    12661 attagatgaa catcagacaa tgcttcaatc aatgattgat cataactttg atgaagtcgg
    12721 atttctttgc acgatgcatt tacatatgat tatcgaggag caagaatacg tgattcataa
    12781 ttaccccgat tattttgaag atattcagga cagacccatc aaataagagg ttttggaatc
    12841 agcgttttga cctgagtatt agagaaagaa acggcaaaat agttcctcat attttgccgt
    12901 tttttttgag ctaataccga gggtcagctg attgcacacc gtttattgag aggttgtgaa
    12961 aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aatagcttct tatgttttta
    13021 acttggctgg acttattacc gcagatgcaa gcttttgaac accgttttcc aagaggtcgt
    13081 gagcctgccg ttcatctccg agtcacaagg aacaatttca aaaaacagct tctcatgttt
    13141 gttgaatatt ttgacttgtg accgaggagt tgacaggaga acatcgttta ttcggaaaaa
    13201 aaggactgtg acacgacttt tgtcacagtc tctttatttg taatctaaat catactccgt
    13261 acatcttttt tctatttttt ttatttcttc taagatccct tggctacgaa tttcattata
    13321 tggccaaagt aagactgcct ctaatacata aggcgaaaaa tcgctttgat tcatctcttt
    13381 tgaaaaactc atcgctgcta tctcgctcaa tgcacgaatg tttgattgat gtgtataaaa
    13441 aggcaaccgc cacagcacaa ttttttcgct ttgcgtataa attcccttaa cagaagcttc
    13501 gatgtggtga aatagttcat gtccaataat caaagcttgc gctgtttctt tagagatcga
    13561 agctagtcct gaacatttta atttttctaa cggctccgtc atcaatcgaa tcccttcatc
    13621 ttctgcataa gttgcaaaag tgattcgttc gtttatttgc tctgattcac aggaaacgat
    13681 tggaacagcc atttgttcac aaatttctcc tacatcggca aaagggtact tttttttgat
    13741 gctacatgcc gtttcgatac cacaatcgat tgattgttgt ataagaattt tctgcttgga
    13801 caaaggaatc tttttccaaa ataaatctcg ccgaaactgg tagaatcccc aaatttcttc
    13861 ggggatctgt tgtaattcgt ttagtaactc ttctaactta tgtttgtttg atatttccgg
    13921 caacgacgat cacttcttcc tccggttcca tagtagatac ttccagatcc atcaaaagct
    13981 gtagttgttc cagatccgtc gctgtaaaat cagagactct tgctcctaag cagagataaa
    14041 attctggtcg ggcaatcaat tcagtttgat aaactgccat atcttcccaa agttgttcga
    14101 cagctgtcat ataattggct gcagttgttt tcatacatga agcatgtcca cgttggacct
    14161 tgataaatcc tttttgatcg atgatccgaa gacttccagc attttttgaa cgattctgtt
    14221 cgccgcatac ataaaagaaa ggtgtatttt ccaataagca aacttcattt ttatttaatc
    14281 gcatatcctc tgcagcaagc tctagtgctt cttctgttgt gatctccttg gttagatcgg
    14341 ttgctttcac ttccgtagaa ccagtagcga tagccgttac ttttgaagtc tgtggatcga
    14401 tttctacgtg gacttcgatc gattctggtg tagcgcctga ttcaatcgct ttattcatcg
    14461 cttcattttt caacgatcgt atatcttctt tacttggcga agggatgata cgttctacaa
    14521 catctcggac cattgcaagt gctacaccaa tagaagaaat cacttcggcg ttttcaggaa
    14581 tcgaatattt cacacccatt ttattactaa agtaagtaat caatgaggca gcgccgcccc
    14641 ctacacctac aagagaaatc tgatctttct ccaagtgata cttgtctgcc aattctaaaa
    14701 tcaccggttc aatcttagca taggattttt ccatgatctg ttctgcgatg tcttcaaccg
    14761 tcgtatgaca ataatcagct aatgcttgga tggcttttcg tgcagatggg acatttccat
    14821 aagaaaaatg ttcttcttgc actaatccaa ggacattggc tgcacatgta tttgtaatag
    14881 tcacttcttc tccgtcttcc atcactacct tgacgtaatc agcagggtct ccttctttag
    14941 gagagaaaaa ttcaacctta ggacctttga ttttttctgt ctccgtaaat actgcataat
    15001 ctagaccggc aatatgtgca gaacgtggac cgacatcgat gattcctgac tgattagccc
    15061 gtaccatcga accgcctgcc acacctagaa cccgaacatc taatgaagag atatatgttg
    15121 ggtggccacc tacaattgaa taatcgattg ctggacgtcc atttttaatg accccgatat
    15181 tggttgttgt tccgcctact tcaaaataaa caccattaga cgctctcaag tacatcaaag
    15241 agcccatgac tgatgctgct ggaccggaaa gcatggttaa aacaggtcgt tttttcattt
    15301 cattgatttc cattactccg ccgtctccgc gcataatcat caaagaaaca ttgacccctg
    15361 cttctcgtac ggaatcttct gtagaggttg ccgtatctag catttttggt aaaatagatg
    15421 cattgattgc tgctgtccga gttcttcttg tcaatccata taattttgtg atgtcagaag
    15481 ccatcgttgt agggatcgat tttaccgatg ctgcttcata aacaactcgt tctggttccc
    15541 cattatctac tccaaatgcc atagaagaaa ccaagacctc cgctctttct ctttctaaag
    15601 aagaaatcgt ttctgaaaca cggtctacat ttaagtgttt gacaggtaag aatgcattga
    15661 caatctctat ttttttctta ttccctaaat cgatatcgtt caatctagtt tgtcgttttg
    15721 ctaaaaaacc ttctaatcca ccttttgcca tcccgattac gccgactttt gctacgtctc
    15781 cttcaatcag tgcattagtc gcttgtgtgg tagaatgtgc aacaaacaca acatcttctg
    15841 gactgatatt attttccagc aaacaatttt gaaaagactg aacgacacct gcggctactc
    15901 cacggacatc atcgtgggtt gttttcactg aagattttcc gatgatttcg tgcgtcgcat
    15961 tatcgattgc gactgctttc gtatgtgtac cgccaacatc aattcccatt cgcacaaaac
    16021 gtttcatttt cttttcctcc attcatctaa tcgtttttat cgacgttttg tttactaggc
    16081 gttcccgtac aataagaacg tgatcaggca taagatcgct ccgatgatcc aaccagttgg
    16141 gatggatttc ttcatatatt ctcgagaagt tacttttgta taacctaatc cccaagcaac
    16201 ccaagattgt gtaatatcta aatgctgagg agcaatcgtt gtgattgcaa ataacgggaa
    16261 caaaaaggct ggcgacatcg ttggattaac agctaatacg acagctaaaa tagctgagcc
    16321 tgaaccgact agattcattg gaccgcggaa aaatcctagt ggtgtcaata tagcaaatac
    16381 gattgccaag actaatggtg attgcggtat ccaatctcca aaaatagctg aaaaataagg
    16441 agaagcatac gcagctgaat tattgaacat cgctaaggtc atcaagaaac caaccattgg
    16501 cgcaacatct actgcgccat ctgtaaataa tttcgcaaac tgacgacaga tttctttata
    16561 acctccgcgc aattttccag taaacagcaa tgcccagata cctgctagaa taaaacctaa
    16621 gatgattgga agatctaaaa gaacgacccc taaaactggt aataatacgg ctaaccatga
    16681 gatcattggt gcgtcgccac cttcagctgg tacgtttgct gccattgcat agcgtttctt
    16741 tttgttcatt gatatattag cgaccgttag taccacaact aagcttacaa tcatcccaat
    16801 cataccaatt tggaaatagt cattataagt atagctttca gccgctggat tgaaaccagc
    16861 ataaatcgtt tgatattgtt tgaaattgac gatattagca aagattccag ccataatcga
    16921 gcccataaat gagaacagtg caaccggtac tgaaatacct aatgacatca agattggcaa
    16981 cacaataact gcaatagaaa tcactggtcc gattcctgtc atcgacatga atataacagc
    17041 ggttacaata catagtaagc tcatcgtgat tcttggcttg tcccctccaa gttcaactac
    17101 ctttcgaatc agggtagctg cgattccgct atctactaga acacgcccga aaaaggcacc
    17161 aaagaagaca tttaccaaga tcgatttggc gtactcagct ggccccgtcg tatagatatg
    17221 cgtcaacaca tcaattactc cttgcccttc catcgccggg tttggtgaaa acgtgtttcc
    17281 gactagcgct aatcccatcc atacagtagc catgatggca aagccgacca tcaagttgta
    17341 gccttttaca cagtaccaga ccatgaaaaa gaatgagata actaacaaaa taccaataat
    17401 aatatcactc atgttattcc tccgtccatt ttgtttttct gataagcaaa ctaatatatc
    17461 agtcaaactc attctaactt atgtagttag cgctttcatt aaagggtgac cataaatgtt
    17521 cagttttcaa ttaaaaaacc ttaattatgc tattttagac ttatacgtta ttgtcctgtt
    17581 tcatttgatg gaggattgta aataaaaaaa gaggaaagaa cataactttt cgttatcttc
    17641 tttcctcttt ataattgttt agcttattta gccaaagctt cttttgcttg gtcagccaat
    17701 gcagtaaatg ctgctgcatc gttaacagcc aagtcagcta acattttacg gttgatgtcg
    17761 atttcagcaa ctttcaaacc gtgcattaat tttgagtagc tcaagccatt catacgagct
    17821 gctgcgttga tacgagcaat ccacaattta cggaagtcgc gtttcttttg acgacgatcg
    17881 cgatatgcgt agttgtatga attcattact tgttcttttg ctgttttaaa taaagtgtgt
    17941 tttgaaccgt agtaaccttt tgctaatttt agcactcttt tacgacgttt acgagttact
    18001 gttccacctt taacacgtgc catgtataat tcctcctaat aaaaataaat cttcagattc
    18061 tgttatttgt acttcttatt tcatttttgc tagttgttgg cgaatacgtt tgaagtcgcc
    18121 actagaaacc atgcttgctt tacgcaattg acggcgttgt ttttttgttt tgccgtggaa
    18181 acggtgactt gtaaacgcgc ggaagcgttt caatccgcct ttaccagtac gtttgaaacg
    18241 ttttgctgat ccgcggtgtg ttttttgttt tggcatgact atatttcctc ctcaaaatct
    18301 ttcgttatcc gactatcggc tgtccaacag ccagttagtc attcaaatta cttactgtcg
    18361 tttttcggtg ccagcgtcaa gaacatgctg cgtccgtcca ttttcgcttt ttgttccact
    18421 gttgcaatat ccgcagtttc ttcagctaag cgattcaaga ctttttgacc aatttcttta
    18481 tgggtaatgg cacggccttt gaaacggata gaagctttca ctttatcgcc cttttccaag
    18541 aacttacgtg cattacgaag ttttgtattg aagtcattca agtcgatcgt aggacttaag
    18601 cgaacttctt ttacattgat cactttttgt tttttacgcg cttcgcggtc tttcttttgt
    18661 tgttcgaaac ggtatttacc gtagtccatg attcgcgcaa ctggtggttt cgcaccaggg
    18721 gcaactaaca caagatccaa gtttgcttgt tctgcaatat tcaatgcttc tgcttttgtc
    18781 ttaactccta attgctcacc atctgaaccg atcaatcgta actctcgtgc acgaatgccg
    18841 tcgttaacca tcatatcctt tgctatggtc attcacctcc aaatattatg agagaaagaa
    18901 aagcttaccg aataaacggc aataaaaaaa cgagttctct tacagaaccc gcgcgtactt
    18961 cacctaatag aaccagtgaa aactatctag cccagtgaca ttatcaacta aggcgagaag
    19021 cgggtgcttc tgcttttatt tctcaaccat catagtatag caaaaactaa tctttttgtc
    19081 aatgtttttc atacagtttg tcttcctttt ctgaaaatga ttagattagc agactttctt
    19141 gcagtgattc ccgcttcttt gctatactaa agaaaaacga ggtgaaccca catgaaaaaa
    19201 atcgaaccta ttccaccttc tcttcctctt cttacaaagg gagagtttta tttagaagat
    19261 gaggagatct tctctggtct tctgatcaac gggggtgacc aaagctatct cgatgccaaa
    19321 aatatggtat taagagagag tcatatccaa aaacttacga tgcaaaaaac agttttacgt
    19381 cattttgaat gcagtaatgt catttttgaa aaatgtgatt tttccaatct ggagtggatt
    19441 ggtggcagtt tccaccaagt cgtgtttcat caatgtaaat taacaggtac gaattttgcc
    19501 gaaagttatc ttcgagactg tactttcact gattgtattg cttcgatggc ttcctttagt
    19561 agtacaaact tgaaagctgt ctccttcgat cattgtcaat tagaagacag cgaattttat
    19621 gaggtcactt ggaaaaacct ttttttgtct gacaatcagt tgaccggttc gaactggttt
    19681 cgaacttctc tgaagggatt agactttacg actaatttct tcacagcaat cgctttatct
    19741 caagattctt tggcgggact aatcgtcaac caagagcaag cacttgtgat cgcccaagcc
    19801 cttggtttgc agatcaaaga ctagatcaac gaatccgccg ccacagtgca ataagcatcc
    19861 aaatagataa gaccaaactg atgatcaagg cgattcccca tccgaaaacg gaatgggtaa
    19921 agggtaactg gagattcatt ccgaagaatc ctgtcacaat agttggaacg gtcaagatca
    19981 gcgaccagac cgtcaaaaat ttcatcgtgt cattcaagtt gttattcagc aaattattat
    20041 aagtgccaga gagctggtct aaaatctgcg aagctaagtt cgtcatttca actgcttgtc
    20101 gtgcttcgat cagcgcatcg tccaactctt ctttttcctt ttctgacaac ttgcgataaa
    20161 tagccaatgc cttgatttgt tctaaaagta ctacattttg ttttgttcct gtaactaagt
    20221 aaaccaaacc gatctctaaa tctgaaagtt gcagcagatt cttgttcgtt gttttttctc
    20281 ttaatttcag attaagccgc tgacgttcac tattcacttc ctcgattaaa ggaaaaaagg
    20341 aatcagaaat caaaaacaat gtacgaaaca ataaactgta tacactcatg tctggcattc
    20401 gttccaaaag cgatcgaatt agtcgaatca cgtactcggt attcggcgtg gtaaatgtaa
    20461 acaaaccgtc ttcttttaaa ataaaagcga taggactagt ttcataatga ttatcgactt
    20521 tttgctggtg cggaacattt gatacaagca gtaacgcttc atcaaaagaa tcgtactcta
    20581 cgcgcgcccg ttcattttta tctagagaat aagtcagcat ctcgtcacta atgccatatt
    20641 cttcttttaa gtttttaaca gctagcacat cgttttgctg tacattgatc caaaaattac
    20701 ggtcttgatt aaaatagtgt ttttcccgca tactctatcc tcctgacatt tgtttccagt
    20761 ataaagaaaa aagtcgctca aataaaggtt taccctttat ttgagcaact gtaaatcaaa
    20821 tggctacttt ggtttggcga atctctgcta atttttcttc gatcattttc aagacttctt
    20881 cttttgcttg aggatcttcc acaaaattat agcggtctcc atcgatttgg atttttggac
    20941 tttctttgta ttcttcatac cattgatcgt aacgtctgtt caattctttg taataatcgt
    21001 atagacttgg atcaaaggac aattgttcat acgaacgacc acgtttttcg atacgctcca
    21061 acatcgtatc aaaagataca cgaatatgaa ctaataaatc cggatgtttt ttatgggcag
    21121 catagggaag ttcttccatc atgttttcca gcagttcatc atacacacga acttctgttt
    21181 ctgttgctct tcctagatct gcatttagat ggaaaagcag tgaatcttca taaatcgaac
    21241 ggtccaatac attattgtca tctttcattg cttgtttgat gcttgcaaaa cgtttattca
    21301 aaaaataaat ttgcagcaaa aaagcatact gttcagggtt tgcataaaat aagggcaaga
    21361 cttcattatc atcaatcgat tcataaaatg cctgactatc aaagtgttca gccatcattt
    21421 ctgttaaact tgattttccg gcaccaattg tacctgctaa aacgatcacg ctcatttacc
    21481 tccagctcac ttcatcacta aatattgtgt ttaactccaa cctttttaaa tataccccca
    21541 atatctagta gtgtcaattg tatttttttc tagtcaaaga agaaaaaaag tcaaaaaatt
    21601 cttaagaaaa aagtgttttt tctttagaaa acgtattctt agttttactt ctgagatggt
    21661 tttagtaaaa taaaggagga atgttttttg agacccttag tggacaaaaa aattcgtgaa
    21721 gcagcgcaac tcgttttggg cgctttattt tttgcttaaa aatcactttt acgtgagaag
    21781 gagttagatt atggcaaact atggccaagg agaatcaagc ggaaagatca tattgatggg
    21841 cgagcacgcg gttgtttatg gagaaccagc gattgccttt cctttctatg caacaaaagt
    21901 caccgcattc cttgaagagc tggatgcaat ggacgatcaa ctggtttctt cctactattc
    21961 aggaaattta gccgaagctc ctcatgcatt aaaaaatatc aaaaaattat tcattcactt
    22021 aaaaaaacag catgacatcc aaaaaaactt gcaactgacc attgaaagca cgattcctgc
    22081 tgaacgtgga atgggatcaa gcgctgcagt cgccacagca gtcactcgtg ctttttatga
    22141 ttacttagca tttcctttgt ctcgtgaaat actattagaa aatgtccagc tttcggaaaa
    22201 aatcgcccat ggtaatccta gtggaatcga tgcagccgct actagcagct tgcagccgat
    22261 ttattttaca aaagggcatc ctttcgacta cttttctttg aacatcgatg cttttttgat
    22321 tgtcgctgat acaggaatca aaggacaaac aagagaagcc gtcaaagatg tcgctcacct
    22381 ctttgaaacc cagcctcatg aaactggaca aatgatccaa aaattaggat acttgacgaa
    22441 gcaagcaaaa caagcaatca ttgaaaattc accagaaacg ttggcacaga caatggatga
    22501 atcacaatca cttctggaaa agctgacaat cagcaatgat tttcttaatc tattgatcca
    22561 aacagcaaaa gataccgggg cattgggtgc caaattaact ggcggtggac gcggtggctg
    22621 tatgattgca ttagcacaaa caaaaacaaa agcccaagaa atcagtcaag cacttgaaga
    22681 tgcgggtgct gctgaaactt ggatacaagg attaggagta catacctatg tttaaaggca
    22741 aagcacgcgc atataccaac attgctctaa tcaaatattg gggtaagaaa aatgaagaac
    22801 tcatccttcc aatgaacaac agcctttcgt taacattgga tgcattttat acagagacag
    22861 aagtcatctt ttctgatagc tatatggtag atgaatttta tctagatggc accttgcaag
    22921 acgaaaaagc aacaaaaaaa gtcagtcagt ttcttgacct ttttcgtaaa gaagccggcc
    22981 tctcactaaa agcttcagta atcagtcaga atttcgttcc taccgcagct ggattagcct
    23041 cctctgccag cgggctagct gctttagcag gcgcttgcaa tactgctctt aagcttggat
    23101 tagatgatct ctctctttca agatttgctc gacgcgggtc tggttcagct tgccgaagta
    23161 ttttcggtgg tttcgtcgaa tgggaaaaag gccatgacga cttaagttct tacgctaagc
    23221 cagtcccttc cgattctttc gaagacgatt tagcaatggt tttcgttttg atcaacgacc
    23281 agaaaaaaga agtgtccagc agaaatggga tgcgtcggac agtcgaaaca tccagttttt
    23341 atcaaggctg gttagattcc gttgaagggg atctatatca attgaaacaa gcaatcaaaa
    23401 caaaagattt ccaacttctc ggagaaacga tggaaagaaa cggactaaaa atgcatggaa
    23461 cgactttagc tgcccagcct ccattcactt actggtctcc tgacagctta aaagcgatgg
    23521 atgcggtgcg acaattgaga aagcaaggca ttccatgcta ctttacgatg gatgctggcc
    23581 ctaatgtcaa agttttagtg gaaaacagcc atttatctga ggtacaagaa acatttacca
    23641 aattatttag caaagaacaa gtaattactg cacatgctgg tccaggaatt gcgattattg
    23701 aataaaggga gatttttatg attgaagtat ctgcaccagg caaactctat attgccggag
    23761 aatatgcagt tgttgaaaca ggccatccag cagttatcgc tgcagtcgat caattcgtga
    23821 cagtcactgt agaatctgca cgaaaagtcg gaagtatcca atctgcacaa tatagtggga
    23881 tgcctgtacg ttggacaaga cgcaacggag aattagtttt agatattcgg gaaaatcctt
    23941 ttcattatat ccttgctgct attcgcttga ctgaaaagta tgcacaagaa aaaaacattc
    24001 ttttatcctt ttatgatctg aaagtaacaa gtgagttaga cagttcaaat ggccggaaat
    24061 atggattggg ttctagcggt gccgtgaccg ttgcaacggt caaagccttg aatgtttttt
    24121 acgcgttgaa tttatctcag ttggagattt tcaagattgc agcactagcc aatttagcag
    24181 ttcaagataa tggttcctgc ggcgacatcg ctgctagctg ttatggtggc tggatcgctt
    24241 tctcaacctt cgaccatcct tggctccaag aacaagaaac tcagcattct atcagtgagt
    24301 tacttgccct ggattggcca ggtctatcca ttgagccatt gattgctcct gaagatttac
    24361 gtttattgat tggttggacg ggtagccctg cctctacttc tgatttggtc gatcaagttc
    24421 accgttcgag agaagataaa atggtggctt atcagctttt cttaaaaaat agtacagaat
    24481 gtgtcaacga aatgatcaaa gggtttaaag aaaataatgt aacgttgatc caacagatga
    24541 ttcgaaaaaa ccgacaatta ctgcatgatt tatctgcaat cactggggtc gtcatcgaaa
    24601 cgcctgcttt gaacaaattg tgtaatttag ctgaacagta tgaaggagcc gcaaaatctt
    24661 ctggtgcagg tgggggcgat tgcggaatcg taattgttga ccagaaatct ggcattcttc
    24721 ctttaatgag tgcatgggaa aaagcagaaa tcactccact gccgttacat gtctatagcg
    24781 atcaaagaaa ggaaaaccga tgaatcgaaa agacgaacat gtttcactag ctaaagcctt
    24841 tcatgataaa caaaaaaacg aatttgactt cgtacggatc atccataacc ctttgccgca
    24901 aatagcagtt tctgatgtcg atctaagtac acaggcagtt gggtttacac taagcagccc
    24961 attttatatc aatgcgatga ctggcggaag cgaaaaaaca aaaaaaatca accaagatct
    25021 tgcaatcgtc gcaagagaag cagatttgat gattgccact ggttcagtca gcgctgcctt
    25081 gaaagtccct tctttagccg atacttatac gattatgcga caagaatatc ctcacgggaa
    25141 aatcattgcc aacatcggtg cagggacttc tgtcgaaaga gccaaagaag ctatccgatt
    25201 gtttcacgca gatgccttac agattcattt gaatgcacct caagagttgg ttatgcctga
    25261 gggagaccgt gattttacca attggaaagt acttattcaa gaaactcaaa cagccattga
    25321 cgtccccctc atcgtaaaag aagtcggttt tggcatgaca agagaaacgc tcaatgatct
    25381 agctgcttta ggagttcata cagttgatat cagcggtcga agcggaacaa gcttcaccca
    25441 gatcgaaaac gcccgtcgct ccaagcggga actgagttat ttagctgact gggggcaatc
    25501 gacagtctct tcattgcttg aagcaaatga agcagacact tctatggaaa tcctagcctc
    25561 cggaggtatc cgcaatgcct atgacatatt taaagccctt tgtctcggcg caaatgccgt
    25621 gggtacttca ggaaccgtac tgacgcatct gatgaatcac ggcgtagaag aaactattat
    25681 actgatgaag caatggcaag aagagcttcg tctgctttat actatggtcg gcgcaacgaa
    25741 taccgcggcc ctacaccaac aatctctgat cttttccgga ccagtgaagg attggtgcga
    25801 agcaagaggg atcgacttag tgaagtatgg aagacgcaca gaaaagagcg tgggacaaaa
    25861 gtaaagaaca cttttgtctc acgctctaaa cacgtacaaa cggcggaagt aaaagcaact
    25921 ccttcggaaa taagctaaaa ttcacaaaaa tttgaaaagc aattttcgtg aattccttct
    25981 tatttcttgg agttaaatgc ttctgtccca gcctcttttt tcgaataaat gatgtttaat
    26041 cagctgtcct tcggtattcg ttcaatccgc tgatttcaca acctctccac taaacagtgt
    26101 actaaagctc ttttaaggtt gttttccgat ataggcaagt atgcctccgt ccacatacaa
    26161 gacatggccg ttaacgaaat cagatgcgct agaagccaag aagacagatg gtccagccaa
    26221 atcgattggc tcgccccagc gtgctgctgg cgtacgtcca acgataaatt gatcgaatgg
    26281 atgacgttca ccgttttctt gtgtttcgcg taatggagca gtttgcggtg tagcaatata
    26341 acctggtcca attccattac attggatatt gtattgaccg tattcagaag cgatgttttt
    26401 cgtcagcatt tttaatccgc cttttgccgc agcataagca ctgaccgttt cgcgtcctaa
    26461 ttcactcatc atggagcaga tattgatgat tttaccgccg cctttttcga tcatatccgg
    26521 aataactgct tttgaaacga tgaatggggc atttaaatcc acgtcgatta cttggcggaa
    26581 gtctgccgca tccatttcga tcatcggtgt ccgtttgatg atccctgcat tgttgactag
    26641 aatatcaact gaacctacat cttctttgat ctgttgaacc atcgcttgaa ccgcttcttc
    26701 atcggtcaca tcgcagacat aaccttttgc atcgatgcct gcttctttat aatgcgctat
    26761 accttcatca actgattctt gattcaagtt attaaatacg atttttgctc ctgctgtagc
    26821 taaagagcgt gcaatctcaa atccaatacc atagacagca ccggtgacta atgctacttt
    26881 tccatctaaa cgaaacatat ccatactaaa ttccatcatt ttctctccct tatgcttatt
    26941 ttaattgatc catttgcacc atatccatgt ctgtataggt gatattttct ccgcacattg
    27001 cccagatgaa tgagtaattg cttgtaccta cacctgaatg gatcgaccag cttggcgaaa
    27061 taatggcttg ttcattgtct acaactaaat gttttgtttc atctggtttg cccatcatgt
    27121 ggaacacacg tgtatcttca ttggcataat caaagtagac ataagcttcc atacgacgtt
    27181 catgtgtatg acaaggcatc gtattccaag cgcttcctgg ttctaaaatc gtataaccca
    27241 tttgtaattg acagctttcg catacatttg gatggatgta ttggtagatt ttacgttcat
    27301 tcagtgtcag gccttcacca gtctccattg gttttacttg gtcgatactg atttttacat
    27361 ttggatattt atgatgagct ggaacagaac taatatagaa tttagctggg ttttcagcat
    27421 cttgagaaga aaatacaaca tgtcgtgttt cttttccgat atagtatccg tcttgttttt
    27481 tcatttcttc tttttgccca tcgatctcga tgaatcctgg tccaccgata ttgatcacac
    27541 ctagttcacg acgctctaag aagtaatcaa cacctagttc tttgtctaat gtgatttcta
    27601 attctttttc agtaggtgtc actccaccaa agatcattcg atcgttgtgc gtataagtca
    27661 aactgatttt ccctggttca aatacctttt ctactaaaaa ttcttttctc aattgttccg
    27721 ttgaataatg acggatatct tctggactgt gtgtataacg tgtttccatt tcttgcattt
    27781 tcttctactc cttctatcga atgatttttc cagagccgca tgctaaaaat tgtgcgactt
    27841 cttcttgact aaattggttg cagtccccat ggatcgtatg cttcaaggca gaagctgccg
    27901 tagcaaaatc aatgatttct tgcggagaca tattggataa aatcccatgg agcacgccac
    27961 ctgcaaaagc atctccgcca cctactcgat ccacgattgg ggtgattcta tggacttggg
    28021 attccacata ttcatggttc atccacaatg tccccttcaa ttcattttca cttgctgaat
    28081 gaacggtacg cttggtcgaa taaaacacct gaatattcgg gaacttttcc tgcatttttt
    28141 gataataata aattagaggt tgttcttctg cttctggcgc tttctcgata cctaacaaat
    28201 aaagagcatc catttttcca gctgagcaga tatctactaa aggtaaaagc tgatggatta
    28261 cttcgctggc ttcttgctgt gtccataatt ttccacgata gttgatatcg aagctgatca
    28321 ggcagccagc tttttttgca gcttctatca cctgtttcgt cattttttgc cattttttag
    28381 acaaagctgg ggtgattcct gaaacatgaa atatatctat tccttggaat aactcctcta
    28441 gcgaccattc caaatgatcc atttcagcaa aacttgatcc ggcacgatca tagatcactg
    28501 aggcggcgcg ttcgccacta ccagtttcca tataataagt acctagtctt gatccaccag
    28561 ataatacctg tttcgtatct actccataac attgcaagtg ctttttgact gcttttccca
    28621 atgcgttatc aggtactttt gtagcaaaaa tcgcttcatg tccgtaatta gctaaagaaa
    28681 ttgctacgtt tgcttcccct ccgccataat gggcagcgaa tcgttcactt gaatcgatac
    28741 gggttccctg ctctgtggac aatcgcaaca tgatttctcc gagtgtcact acttttccca
    28801 taagcagcta cctcttgatt tctttcattt ttgctgcata ctgttgcgcg acttccgtta
    28861 ctttgtcaaa atctcctgta gctgcgggtg ctaaaaggtt cccacctact ccgactgtta
    28921 cgacaccagc atcgaaccac tctttcatat tgtccagact cacaccgcca gttggcatga
    28981 tattgacttg aggcatcggc gccttgaagg cagaaatgat acttggtcca taagcgcttc
    29041 caggaaatag tttgacgata tctaccccgc ttttcagcgc tgttttgatc tcggtaatag
    29101 tcatacatcc tggtaagtaa ggaatttgat acagattgca catctctgcc gtttcttgat
    29161 caaaggaagg gctaacaata tactctgcgc cagctaagat agccaagcga gctgtgacag
    29221 cgtctaatac tgtgcctgcg ccaatcacta tttctggttg gtctttgtag aatgacaaaa
    29281 gttctttgat tacttgatct gcttgaggga ctgtaaaagt caactcaata ccagttagac
    29341 cacctttgat gattgcgtgg caagcattca aagcttcttc tttgcttttt ccgcgtacca
    29401 ccgcaatcac tccggcgttt ttcaatcgag ataaaatatc aatttttttc atcttctcca
    29461 cctatttcct atttagaaat tttattttat aacttggaaa tctataccca tattatacgg
    29521 cagaaaagaa aaattgcaaa cgcttttttc attatttttg aattctttgc tcgatttcgc
    29581 tttttgtttg cttgagggca ctgattattt gttcttttgt ctcatctgtt aaacgatatt
    29641 tcggcatact tacactaaat gcaccaatga tttgttcgtt tttcacgatt gaggctccta
    29701 tacaaaaaat atcacgttcg atttcttcat cgtcaaatgc gatgtgggtt tcttttaccg
    29761 ttttgatttc ctttttcaac cgtaatgcat tggtgatcgt atattctgtg taagggatca
    29821 acggatgctt ttcaagataa tcagtgtact gctcttcact gaattctgct aatacggctt
    29881 tgcccatcgc agaattgtat aatggtcggg tgattccgat ttttgaagac atgcggatcg
    29941 tttgatgttt tggatctagt ttgttcacgt acaagatctc attgttgtcc aataccccta
    30001 agtggatagt ctcatcgatc ttttgctgca gctgttctaa aaacggtaaa gtcaactcta
    30061 ccaaatcgat ttgttcaatg tttttattcg cgtagcgaat aagctttgca cctaaacgat
    30121 agtttttctc tgaattttta tttacgtagc cgatcatcaa caaagtatct agtattttca
    30181 agactgtaga agaggtcatt cccacaccct ttgcgatcgt ctgcaaagag acatcttggt
    30241 tttccgctaa atagtctagt attttagagg ctttgatcaa tacagttcca taaggctttt
    30301 tattttccat ataatattct cctatttctt tataagaaaa attatagcgg aaataccatc
    30361 aatttaaaag agatatccag atataaacta ccatttctct ttacaggcaa tttaatgttg
    30421 aagatccgct tcgtttttac atcaaacttt gatataaagc aatgatttct tctactgtcg
    30481 tttctcttgg atttcctggt gtgcatacat ctcgcaaagc atcttctgca attgctggaa
    30541 tatctgcttc ttttacacct aattcagaaa tggtttctgg gattccaacc gctttgctca
    30601 accgatcgat ctcaccacaa gccgcttctc ttacatcttc taaagacatt tcagctgctc
    30661 ctttaatccc tagaacaaga gcgatctcac ggtacttttc tccagtatat tctttgttat
    30721 atttcatgac agttggcaaa agtgctgcac acgctacgcc atgaggaatg ttgtaccatg
    30781 cacttaacgg gtgagccatc ccgtgtacca gtcctaaacc gacatttgag aagcccattc
    30841 ccgcaatata ctgtcctaat gccatttttt ctcgcccatt ttgatcgcca tctacagaag
    30901 cttccaaaga atggccgatg atttcgattg cttttaaatg cagcatatca gtcatttccc
    30961 aagcgccttt tgtgataaac ccttcaatag catgcgtcat agcatccata cctgttgatg
    31021 cagccaattt tttcggcatt cccatcatca tatcgctgtc gatgaacgcg acgattggga
    31081 tatcatgagg atcaacacaa acaaattttc ggtgatttac ttcatctgtg atgacgtagt
    31141 tgatcgttac ttctgctgct gttccagaag tcgttggtat agctaatata ggtaaacatg
    31201 gatttttcgt atcagcaaca ccttctaaac taatgacgtc tgaaaactct ggattcgtca
    31261 aaataatccc aatggctttt gccgtatcga ttggcgaacc tccaccgatt gcgatcaaac
    31321 agtctgcctc tgcctcttta acaaaagcca ctccatcttt tacattttgg atcgttggat
    31381 taggtacgat cccgtcgtat aaagcataag cgattgctgc tttatctaga agttctgtta
    31441 cttttgttgc tataccgtgc tcgatcaatc ctttgtcagt aatgacagct gctttgccga
    31501 atcctcgctg ctgaaattca gggacgatcg actcaatcgc tccttttcca aaatacgatg
    31561 tttcatttaa aatcattcga ttactcattt tctattcctc ctaatttgta ttatttttca
    31621 caataaaaac gaaagaaccc caagcaaccg gttgcccagt tgcttgttgc tacagttcaa
    31681 agactggcat caattcccaa ctgactggag aggcatctgg atttgtctcc atgatatctt
    31741 ccatgtactt ccaccatttt tgattgatag atgtatttgc cattttttcc cataactttt
    31801 catcttcgat cactaagtaa ccaaataaca aacttgtact cggatcaaga tagatcgaat
    31861 agtcgatgac cccgtgttca cgaagtgctg tacgcatttc tggccaaagt tcatcatgtc
    31921 tttttttgta ttcttcttgt ttttctgggt aaactttcat gcaaaaagct tttttcacca
    31981 tgtcagacac ctgctttcgt atgaacttcc tccaaataac ctgcttttgg agtgacacca
    32041 aacgcatctg ctaaatgcca caggttttcg tccgtgattg tttgcaagat tggtccttgc
    32101 gctttgacgt acgtatagac ttctgctgcc ttttctgctg tctcgatcaa accaaagact
    32161 tcatccatgt cttttcctga cccgtagatt ccatgttgtg gccataaaac caatcgagtt
    32221 tctttcattt tttcagctgt tgcttcccca atctcatttg ttcccggaac catccacgga
    32281 atgatggaaa ttccttctgg aaaaacaact aagcattccg tacacatttg ccatagcgta
    32341 cgactaaagg aacgttcgtc taattcatgc gtaaaactca tcgctaatag atgactagca
    32401 tggcaatgca taacgatccg attatctggg tctacttcta aacgggcgat atgactcatg
    32461 aaatgacttg gcaactcgct tgtaggaagg cattcgtttt ccagccccca caaaagttcc
    32521 agtgttttgc catcttcagc aactcgaacg attccaagat tctcggcagg ttcgctcgct
    32581 acatttttaa aatatcttcc tgaaccagtt acaataaaat agcatcctgc taaacggctg
    32641 gcatcaaaaa tcatcgagat gcggcggatc actttttcag gatccaagaa cggtgtgatc
    32701 tcttcttctt ttagtaaata agaaatattc ccgccatttc tttcatccca tcccaagcga
    32761 taaagattcg acgtcgtttc gatcatttct ttcacataag gcgcttgtaa aatatctatt
    32821 tttttcaata tagccacact cctattttct taattccaac acgtcttttt gatattcctg
    32881 gacttcttcc agccaatcca aaccaactgg aacttgattt ttgtaacaga aataattcca
    32941 tacatcggca tacgggaaat ctttcaattc ttccgtataa gccattcttg aagtaaagtc
    33001 aaaagccagc tccatttctt tcaatcgctc aactggttcc agcatagctt tcagcagtgc
    33061 tttttgtgta tttctcgtac cgatgaccca agcagcgata cggttgatcg ttgcatcaaa
    33121 gaaatccaga ccgatatttg ttttttccaa caattcgtta cggactaatt ctttagcgat
    33181 ttcctgcaat tcatcatcca tcaaaaccac atggtctgaa tcccaacgca ctggacggga
    33241 aacatgcaac atgattcctt tgctgaataa agctaatgaa gataatttat tggaaatcac
    33301 ttctgttgga tgaaaatgtc cagcatctag acaaacaagt ttgtcacgag tcaacccgta
    33361 acccatataa aattcatggg aaccgactgt ataagcttca gcgcctaaac caaatagttt
    33421 gctttctact gcatcttgta cataccgttc atctaactct tctgtaaaaa tttcatcgag
    33481 tgattccatt aaacgttccc gtggggtaaa acgatcaatc ggattatctt tcatcccatc
    33541 aggtacccaa aagttattga tacttgtttg cccagtttct ttcccaaagt actccgcgat
    33601 tttgcggctt cttttcccgt gttcgatcca gtattcacga acttctttat ctggatgagc
    33661 caatgtaaag ccgtctttca tcattggatg cgaaaagaaa gtcggattga agtcaagacc
    33721 gatttcttct ttttttgccc actctaccca tttttcaaaa tgtttcggct caatttcatt
    33781 tagatcgaca gattcttctg tatccaaata gatagcatga aggttcaatt tgtgtttgcc
    33841 cggtatcaac gaataagcct tctccagatc ttgtctaagc tcttcaggtg ttcgtgcggc
    33901 tcctggaaaa ttccctgtcg ccatgattcc accggtcaat tcgccatccg gtgtcaagaa
    33961 acctttgaca tcatctcctt gccagcaatg catagaaatt ttgatgtcct gtatcttttc
    34021 tagtacagca tctgtatcta caccgatagt tgcatatttt tcttttgctt gttcgtaatt
    34081 tctttcaatc gtgttcatac gattactcct ttgattttat tcacgtacca ttccatcgtg
    34141 ttttgcttat tttcatctgg gtaaaaagtt tcacatggaa acgactgttt gattacattt
    34201 cttgcttgtt ctatattcga aaactgacct gtactgagca tttgcatggc aacattcccg
    34261 attgctgtgg cttcactagg tcctgcttct acaatgattc cggcataatc tgctgtcaat
    34321 tgattcagta gacgattatt cgatccgccc cctacgatgt gcagcttttc caatttcgtt
    34381 ccagttaagt gttctacttt gatcagctct tgtgcgtaac aaagagcaag attatcatac
    34441 acacaacgag cgatagctcc cgatgagaca gggactggct gctctgtttc aaaacagtat
    34501 tgctgaatct cttctatcat atttggggga ttcaaaaatc gagggtcatt gatttccatc
    34561 acttgctgca gcggtttttc ttttgcagca agctctgcca attccgcata actgtactcg
    34621 tactcttgca ttcgggcaac ttcctggatc aaccacatcc ccatgatgtt tttcaaaaaa
    34681 cgcaccgtgt tatgggcacc ccattcattc gtataatttt cagaaaaagc attgcgagaa
    34741 acattggcga tctttgtttc aatccctaaa agcgaccatg tcccactgct gatataaccc
    34801 caacgttctc cttgacctgg cgttcctaat acagctgacg ccgtatcatg acttgctact
    34861 gtgataaagg tcacatctgg caaatcatat tttgaaaatt gttctttttg cagatctcct
    34921 agtatcgttc ccggctctgt caatggtgga aataattcct tcgagacacc gattttttca
    34981 agaagttcta tttcccattc ttttgtccct gcattcagca gctgcatagt agatgcatta
    35041 gttttctccg tcaccatgtt accagtaaag acatacccta aataatcagg tatcagtaac
    35101 aatttatgtg cttttgccaa attttctttt tcttcagcaa acaattggaa caaggtattg
    35161 aaaggctgga tctgaatccc tgtctgctca tacaacttat ctagagaata gatttcagaa
    35221 aactgttcta ctgctccatt ggtacgcttg tcccgataag caatcggatc atttatccga
    35281 tccccttgtt catcaatcag acagtaatcg actgcccatg tatcgatccc cgcaaaaagg
    35341 cgatcaatcc ctgtctgctt taatttttca aatccaatca aaatctcttt gataagataa
    35401 tcgatcttcc agcgatcgtg accgtctttg ttccgaaagc cgtttttgaa ccggtgtact
    35461 tcttccaaga tcaaagaacc ttttgcatcc ctcgtacttt tcattagtcg tccgcttgat
    35521 gcaccgatat cgatggcaac aaatgttgga ttcatttctc cacctctttt actttattgt
    35581 tgattagcac tttattaata aagcgttatc atttattgac aatgctatca taatacagaa
    35641 tcttatattt tgcttcacac tattttggta tataattgca ttatcttgac aagttgggag
    35701 tgtttacatg gattactggg aaaaagccaa ccactcatgg tcggaagact caaaaagagt
    35761 gatcttgacg cctacacaaa gaagcaggaa tttatttttt tatatccaag aaatcggtta
    35821 cttcaaagca ccaatccctt actatactga acgagcacat ttaccttctt acttgataaa
    35881 gtttactttg tctggcaaag gttcgcttac atataatgat aatcagcaac agctaaaagc
    35941 tggagactta ttttttattg actgccaaga atatcagcgt tatcaaacga tcagcgaaga
    36001 accatgggaa atggattgga tccatttgaa tggtcagaac gtccaatcat tttatcagga
    36061 gttcacaaaa gacggccata acaccttcca ttcaacagga gatccatcag aaaacaagat
    36121 ccaccatttg atcacagaat tgatccacat gcaacaaggc acgaatgccc gtaccgattt
    36181 ccagtcctct gtagctatcc acgagttgct gaatgaactg attttgcaaa agtatcagct
    36241 agatttcgaa gaagaggata ttcccgccta cgtttacaaa ctgaagcaat acttagacga
    36301 acatttcaaa gaaacgatta cacttgatca attagaacaa caattcttat tgaataaata
    36361 tcagctgagc aaagattttt ccaaatttat cggttctcca ccaatcgatt atttgatcag
    36421 caaaaaactt tcttacgcta aagaccttct tcggtataca gaatttacgg ttcgaatgat
    36481 ttccgaagaa atagggattg agaattttgc ttactttagc cgcctgttca aagcaaaaac
    36541 aggactatcg ccgagagact ttcgtaaatt cagctgattc atataatatg tacgtcattt
    36601 tttcaaaata ttttgaaata tatagataaa aataaaataa ccgcagtttc tgcttctatg
    36661 taaacaacag cagctactgc ggttattttt tagtgatcat tctcattgtt catcttcaaa
    36721 atgttcataa tgaaccaact gtttctgtgt atcacgtttc aaaaattctt taggcgttat
    36781 gccgaactct ctcttgaatg ctttcagaaa atgcggataa gaagaaaaac cacttttact
    36841 atatacagca ctagagctgc ggtattctcg caacaagtgc ttggcataga tcagcttttt
    36901 cttcattaca tactgatgaa acgtaaatcc agtgtacttt ttaaattctc gtgtgatatg
    36961 gtatttgctg acaaaaaact gcttttccat ttcctctaaa gaaagcggat gagataaatt
    37021 ttttgagata tattgtaaca tttgttccac acgctcatta ggaactacag cattctgttc
    37081 ttgcgcactc tcttcttttt gtacaacttg attgagaaaa atcaagtatt ctaccagagc
    37141 ttgttcatat tttatgtctt caccgtaaat ttttttatca ggaggatggt cgataaatga
    37201 taatttttgc ttcaatactt cctgttctat ttgcaataca cgactcctct tctctcccat
    37261 tggttgaaaa caagccgata gattagacca tggcgtggaa cgactttcta gaaaagaagg
    37321 ggtgacaaag atatagacac gctcgaatac atctgaagat tggcggacga tacgatgcaa
    37381 atcattggaa tggatgagca gcacacttcc tgctttgatc gtaaattgtc gtccgttcaa
    37441 ataaaaagtc gcttctccct ttaatgtagc atggatctca tagaaatcat gatgatgata
    37501 gatcgtttta ttttggctga cgacatcttt gacatgatag atttcatact gatcagaaac
    37561 catcgtattg acttgttcac cgctgatttt cttttggagc atgtgccgca cctccttttg
    37621 ttcacttatg cacaatttta ttataccacg ttttttgtaa tcaaaaagag ctgcttttat
    37681 atactcttat aaaaaagaag agacgtggca tcttctagcc ccgccccctc cattctaaat
    37741 attagttaac caacgtcttc tttacattat tcaagaagac ttcttccata acgaatccca
    37801 tcatcttttg tttcttcaag aaccacatac aggtacggac gattcttcat gatgatttct
    37861 ttgatttttg tgtactcgat cacaccgtct cccaaattta ccggcacgat tttttcctgt
    37921 tcgacacgaa aatctttcaa atgaaccgca catatctttt caccaaaacg ttcaaaagct
    37981 tcttctacca attggacttg ttcttgataa tcactacttg tgatcaaatt ggtcggatct
    38041 aaaatgattc ctagatagtc cgaatctact gcttggatca gctgctctac ccttgctaaa
    38101 gaatacaccg gatgattcaa acctggttct atccccacca tcatttggta tttttctcca
    38161 gcttttacca agcgacggat aacggacaca gcttcagcaa atgcttcatc tgtgaaattt
    38221 tcttccgtat attggatttc cggcaaaaca catccagttt ccgaagcaac catcgacgca
    38281 ccaaaggaag aagcatagcg aagatactgc tcgaactttg tcagcaactc ttgtcgtatc
    38341 gttaaatctg gatgaatcat attgatatag caacttaaaa ttcctacggt cacttcttgc
    38401 ttttctaaaa ctcgtttgac ataatttccc atcccagaat tgatctcatt tgcgccagaa
    38461 ggtatttcag gaaacgaaag acctaaggcg agctgcagtg tatgaatccc ttgttctttt
    38521 gttttttctg caagatcttc tatcgtttgg acttcggaaa gatcatgtcc tctaattgta
    38581 atgttcaaca taactattct cctaactcag catattgggt agccacatga cgatttgcgg
    38641 gaagtaagta atcaaaaaca agacgactaa gataacccca taaaaaggaa gaagcggttt
    38701 cagcattgcc tctgctttta cgcctcctac acttgcaccg acatatagtc ctgtacctac
    38761 tggcggcgtg atggagccta cacacagatt catgattgaa aacaacccgt aatgtacagg
    38821 atcaacccct acacttgtga cgattggtaa aaatattggt gtaaaaatca agatagcagg
    38881 cgccacatcc ataaacatac ccaccagtaa taacacaaga ttgactaata acagaataat
    38941 aaatttatta tccgttacgc ccataatcaa cgaactaatt gcctgaggaa taccggtaaa
    39001 cgccatggcg aaagacatca tggaagaagt agccaataag aacatgatcg tccctgacat
    39061 tgctactgat tgaaacaaca tattaggtat atcttttagt tttaccgttt tatagtaaaa
    39121 catagcaatc aacagggagt aaatgacagc tatcgctgaa gcttctactg ctgtaaatat
    39181 ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa tcgcctcttt
    39241 tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt tattcttttt
    39301 agcaacgatg aatgctacta gcatgattcc tagcgcccac aaagcgccta cgagataacc
    39361 tcccataaac aaagcagaga tcgaagcacc accagcaaca agtgaataca tgataaaagc
    39421 agtacttgga ggaatgacca tacctgtcgg agctgacgcg atattgaccg ctgtcgcaaa
    39481 cttccggtca tatccttctt ccacttgctg agggatcaac actccgccaa tcgcggtaga
    39541 agcggcaatg gccgagctag agatggaacc aaataaagca ttccctaaca cattcgtatg
    39601 agccaaagcc cccggcacgc gcccgacgaa caacatcgca aaatccacta gtttcttagc
    39661 tatccctcca ttattcatga taatgcccga gagaacgaaa aacggaatag cgatcagcga
    39721 aaaactattg atacttccca ccattttttg agctgaacta aaagcagaaa catcaaacgg
    39781 tataactaac aataaagtca tcatcgatga gaatgcaata cttatagcaa tcggcatccc
    39841 taatacaagc aacacaacaa atacaaaaaa taacactaat ccggcttcta ttgccatttt
    39901 attcctccat ttttatattt tttcttgaat tgctggcttg ttgcttaatg tatcgattag
    39961 attgatcagg ctatagataa tgatgagtac ccccgataca ggtactgaca gataaacata
    40021 tcccatagga atacttaaag aaggcgagat ctgccccatt gtcaatgtga cagcattcca
    40081 tccgccatac accatgatca tcgcagcaaa taccagaaat aaactttgaa caacaacttc
    40141 taaaatttta ctttttttgc cagttaattt ttcacttaaa accgttacgg ctaaatgtgc
    40201 tttttgtccc acaacatacg cggaagacaa catggcaaac cagacaagac cgaatcgaac
    40261 aagctcttcc gtgaccgtat ttggatttcc taaaaccgtt ctggaaaata cttgccataa
    40321 aacaacagcg accatcccaa caagcaaaac agctcccagg atctctaata ccttgtttaa
    40381 aatcattttc atcacagcta cgctccttgc tcttggatct tttgataaag gtctttgtag
    40441 gtaggatcgt ttttgaatat atcatgaagt ggggcaactg cctctcgaaa cggtgtatta
    40501 tctacatcat tgaattggac tccatgttct tcttgtgcta aagctttttc ttcttcaatt
    40561 gcttttgcaa aagcttcaat ttcaaaaacc gttgattctt tcgctgcttc ttcaatggct
    40621 ttttcctgct catcagttag ctgttctttg atggcatcat tcatgatgat gatatctggc
    40681 acacgtgtat gctcatcata ggaatagtat ttagccacac caccatgtcc agctgtatac
    40741 aagacaaatt cattgttttc tgatccgttg atcaaattgg attgaagaga cgtgtacact
    40801 tcatcacttc ccataggtac gggcgaaccg cctaaaagct ctaccatttt gatagcggtt
    40861 tcgctttgca tcacacggat cttctttccc ttcaaatctt ccggcgtatg gatcggaccg
    40921 tctaccatat aaaagctgcg ctgaccagaa tcataatagg tcaatccggt aaatcctaaa
    40981 tttttggtcg attggtagat tggctccatt atttcttcat tatccatcac cttaaagaaa
    41041 tgttcttcat cattgaatag ataaggaact gaaaaaatcg aataatcttt agagaaactt
    41101 tccaacgctg aggcacttac ttttgtgaaa tctactgctc ctgactgtgt caattcgatc
    41161 agttcacgtt ctccacccag ttgtccatct gggaaatact ggatctgtac ggaaccatct
    41221 gttttttctt ttactaaacg cccaaattca ttcattgcat caattactgg ctggctgttg
    41281 cttgcataag caaagcgcaa ggtaactatc tcatttacat ctgcatctgc gacagttgcc
    41341 acactagaat tatttccgca gccgcttaga cctagcagac ctaggctaag cgaacttgct
    41401 gcaaacagca gtgatgtttt tttcatatgg gtttcctttc ttttaacaat aagagacgag
    41461 caattctgtc aaacaaagca ccgttaacga ctgtccataa ggcattgctg tgattccgat
    41521 attccgataa aaatctaaac tgtcccccat tcctgtcccg attgaaacgt tttgaacttc
    41581 tcctttttca tcgatttgtt caagcaatcc ttggatagca cgataagcga cttctttata
    41641 ttcttgtggt aaataacgtt tatggaccgc tttcaaaata ccgtaagcaa atcctgcagt
    41701 tgcagaagat tctacgtaag aagaaggatc atccaacaac gtatgccata aaccgctttc
    41761 agactgataa gtttttaatg cacgcacttg tgcttcaagt gtttcgatca ataatttgcg
    41821 caatgcatct tctttcgata attctaagat ttcaatgatc tctggaatag caatcgtcaa
    41881 ccagcagttt ccacgagccc aaaatgcttc ggcatagtta tgatttcctt caaacgtcca
    41941 gccgtgatac cacaatcctg ttcgtttatc catcaagtat ttgatatgga tcataaactg
    42001 gtaacgagct tcttctaaat attctgggcg attcaacagt ttcccgattt ttgccaaagg
    42061 aagcactgtc atcatcaatg tatcgtccca caactgattc ttattttccg gtccatatgt
    42121 ggcatgctgt aacccgtctt catttgtccg aggcatgtca ttcatgaccc attctgccca
    42181 ttgttctaaa taaggcaaat agcgtgaatc tttcgtgtct tcatacaagt aagccatagt
    42241 caatagcggt gccattgtat tgacgttttt gggcggagcg ccttcttcca tacgatcctc
    42301 aaaccattca ttgaccactt gataagcgcg aggatttttt gtcatctggt aatttttata
    42361 aataccatat agaccgacac cttgtggcca attccacact gtccagcttt tgtcatctac
    42421 tttcagtcca tcaaagtcca acaaaaattc accattatcg tctttgatcg ttgtcaggtt
    42481 ttcaacgagc aggtcaattt tttctttcac taattcttta ggtacaaaaa tctcttcttt
    42541 aataatcatc tttacgccac ctttttaaac gctttcattt tatatcctta atatagcagt
    42601 aataccgtcc actataaata tcgctttttg aacaaaaact gtctaaaatt gacctaaatg
    42661 ttaaataaca aaagtaactt tcagttactt ggaaattaaa aaaagaactt ggagaaacaa
    42721 cgttttccaa gttctttttc atctattttc ttatcttttt tctacgcttt cttttgctta
    42781 tccgttgtaa gcatcaatga tgatctgttt caattcagag atcaatggtt ctttcggatt
    42841 agctgtcgta cattggtctt cgtaagctaa ttcagccata cggtctacgg ttgtatctag
    42901 tgtttcttgt gtgactcctt gagcttttag actcatgttg atgccgacag attttcctaa
    42961 ttcataaaca gccgttgcta aagcttcgac taattcctct gttgtttcgc ctttcagacc
    43021 taagaatttt gcgatatctg cgtaatctgt atctgcacgg aaatagtcat atttagggaa
    43081 catcgcatgt ttttgtggat cttttgcatt atagcggata atatgcggta aaagaatcgc
    43141 atttgtccgt ccatgaggga tcccgtattc cccgccgatt ttatgtgcga ctgagtgaca
    43201 aatacctagg aatgcgttag caaacgccat accagccatt gtagaagcgt tgtgcatttt
    43261 ttcacgtgat tccatatcag gtgtttttac tgatttttct aaatagtcaa agactaactt
    43321 gattgcttgt aggctcaatc cacgtgtata atctgaagcc atgactgaaa cataggattc
    43381 gattgcatgt gttaacacat ccatcccggt atctgctgtg acagatgctg gaactgacat
    43441 gacgaattga ggatcgacga ttgcgacatc tggcgtcaat gcataatctg ctaacggata
    43501 tttcacatgt gtttcactgt cagtgatgac cgcaaatggc gttacttcag aaccagtccc
    43561 tgatgttgtc gggatacata cgaattgtgt tttttctggt ttgtcgatct tatatgtccg
    43621 tttacggata tccaagaatt tttgttttgc accaaagaaa gatgtgtctg ggtgttcata
    43681 gaacatccac attcccttag ctgcgtccat tgcagaaccg cctccaagag cgattactgt
    43741 atctggcttg aagtctgcca tcagtttcgt tcctgcgtat acggtatttg tcgatgggtt
    43801 cggttcaaca tctgagaaga cttcgatctt cacatcattt ttacgtcgtg ccaatacttc
    43861 acgaactgta tcgcagtaac cgaattggac cataccaggg tcacaaacga tcatgacacg
    43921 ttcaacgttt tccattttct ctaagtacaa tagagagttc ttttcaaaga agatttttgg
    43981 cggtaatttg aaccattgca tgttatttct ccgtttcgct acagttttga cattgattaa
    44041 gtttacagca gagacgttac gagaaacaga gttcttacca taagaaccgc atcccaaagt
    44101 caatgacgga atcatttcgt tatagatatc accgatgccg ccttctgcag atggtgtatt
    44161 cactaaaata cgacaagctt tcattcgaag accgaatttg acatgcaact cttcgttttc
    44221 tgaatggata actgctgtat gtcctaatcc tccaagattc aacatacctt cacacaaatc
    44281 aaacgcatgg tccgtgttgt tagctttgat catggcaaga actggagaaa gtttttctct
    44341 tgatagcgga taatctgggc ctacgccttc caattcagca atcagcattt tcgttccttt
    44401 tggtacactg attcctgcca attttgcgat ttccatcgca gaatgtccaa cgattgatgg
    44461 attgaccgca tattttcctt cgttcatcac tgcatcttct aatttttgca attcatctgg
    44521 ttttacgata taaacttggt gtgcttggaa ttctgctttt actgctgcat agatttcttt
    44581 atccacgatc acagcttgtt cagaagcaca aatcattcca ttatcaaatg ttttcgaaac
    44641 gatcaaatcg ttcactgcac gtttaatttt tgctgttttt tcaatataag ctggtacgtt
    44701 acctggacct acacctaaag ctggttttcc tgtagagtaa gcagacttga ccattccagc
    44761 tccacctgtc gctaagacga tcgcgatacc tggatggttc attagcatag aagtcgcttc
    44821 gatcgaagga tgttcgatcc attggataca attttcagga gctccggcag cgatagcagc
    44881 atcacgtacc actcgtgctg cttctgcaga agatttttgg gcacttggat ggaaagcaaa
    44941 aacgattgga tttcttgttt tcaacgcgat cattgatttg aaaatcgttg tggaagtcgg
    45001 attcgttgtt ggagttactc cgcaaaccac accgactggt tcagcaattt caatcaaccc
    45061 tttttggaca tcttcattga tcacaccaac tgttttatca tgtttgatat tatgccaaat
    45121 atattcggac gcatacatat ttttgattgc tttatcttcg taaacaccgc ggcctgtttc
    45181 ctctacagcc atcttcgcta atggcatatg ttgatccaaa gctgccattg ccatttcatg
    45241 cacgatatgg tctacttttt cttgatcaaa gctttccatt tctttcaatg ctgcatttgc
    45301 tttttctgcc aaatcatcaa tcattgcttg tacgtcaatt gtcttcgttt tttctttttc
    45361 tattacttta gccatcatta tcctcctaat gggtcatggt tatttgttaa ataattcaca
    45421 tgtatatcat acaatattta tttttctatg taaacacttt tttgtgattt ttttcacagt
    45481 tatttttaaa tataaa
//
LOCUS       DAZKOK010000011        44854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000011 DAZKOK010000000
VERSION     DAZKOK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.011"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..555
                     /locus_tag="ACG90W_000555"
     CDS             163..555
                     /locus_tag="ACG90W_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0476947.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            583..1923
                     /gene="glnA"
                     /locus_tag="ACG90W_000556"
     CDS             583..1923
                     /gene="glnA"
                     /locus_tag="ACG90W_000556"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0476948.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2649..2894
                     /locus_tag="ACG90W_000557"
     CDS             2649..2894
                     /locus_tag="ACG90W_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476949.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3116..4726
                     /locus_tag="ACG90W_000558"
     CDS             3116..4726
                     /locus_tag="ACG90W_000558"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0476950.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4703..4915)
                     /locus_tag="ACG90W_000559"
     CDS             complement(4703..4915)
                     /locus_tag="ACG90W_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476951.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5018..5887
                     /locus_tag="ACG90W_000560"
     CDS             5018..5887
                     /locus_tag="ACG90W_000560"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0476952.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6052..7323
                     /locus_tag="ACG90W_000561"
     CDS             6052..7323
                     /locus_tag="ACG90W_000561"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0476953.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7320..8615
                     /gene="rho"
                     /locus_tag="ACG90W_000562"
     CDS             7320..8615
                     /gene="rho"
                     /locus_tag="ACG90W_000562"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0476954.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8714..8977
                     /locus_tag="ACG90W_000563"
     CDS             8714..8977
                     /locus_tag="ACG90W_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0476955.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9422..9871
                     /locus_tag="ACG90W_000564"
     CDS             9422..9871
                     /locus_tag="ACG90W_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0476956.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9928..11346
                     /locus_tag="ACG90W_000565"
     CDS             9928..11346
                     /locus_tag="ACG90W_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0476957.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11346..12206
                     /locus_tag="ACG90W_000566"
     CDS             11346..12206
                     /locus_tag="ACG90W_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0476958.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12380..13153
                     /locus_tag="ACG90W_000567"
     CDS             12380..13153
                     /locus_tag="ACG90W_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0476959.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13300..13665
                     /locus_tag="ACG90W_000568"
     CDS             13300..13665
                     /locus_tag="ACG90W_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0476960.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13813..15021)
                     /locus_tag="ACG90W_000569"
     CDS             complement(13813..15021)
                     /locus_tag="ACG90W_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0476961.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15040..15318)
                     /locus_tag="ACG90W_000570"
     CDS             complement(15040..15318)
                     /locus_tag="ACG90W_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476962.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15511..16167
                     /locus_tag="ACG90W_000571"
     CDS             15511..16167
                     /locus_tag="ACG90W_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0476963.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16609..17406)
                     /locus_tag="ACG90W_000572"
     CDS             complement(16609..17406)
                     /locus_tag="ACG90W_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0476964.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17403..18587)
                     /locus_tag="ACG90W_000573"
     CDS             complement(17403..18587)
                     /locus_tag="ACG90W_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0476965.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18612..19859)
                     /locus_tag="ACG90W_000574"
     CDS             complement(18612..19859)
                     /locus_tag="ACG90W_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0476966.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20074..21819
                     /gene="dnaX"
                     /locus_tag="ACG90W_000575"
     CDS             20074..21819
                     /gene="dnaX"
                     /locus_tag="ACG90W_000575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0476967.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21839..22153
                     /locus_tag="ACG90W_000576"
     CDS             21839..22153
                     /locus_tag="ACG90W_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0476968.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22178..22774
                     /gene="recR"
                     /locus_tag="ACG90W_000577"
     CDS             22178..22774
                     /gene="recR"
                     /locus_tag="ACG90W_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0476969.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22838..23482
                     /gene="tmk"
                     /locus_tag="ACG90W_000578"
     CDS             22838..23482
                     /gene="tmk"
                     /locus_tag="ACG90W_000578"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0476970.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23497..23826
                     /locus_tag="ACG90W_000579"
     CDS             23497..23826
                     /locus_tag="ACG90W_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0476971.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23840..24778
                     /gene="holB"
                     /locus_tag="ACG90W_000580"
     CDS             23840..24778
                     /gene="holB"
                     /locus_tag="ACG90W_000580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0476972.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24814..25638
                     /locus_tag="ACG90W_000581"
     CDS             24814..25638
                     /locus_tag="ACG90W_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0476973.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25631..25978
                     /locus_tag="ACG90W_000582"
     CDS             25631..25978
                     /locus_tag="ACG90W_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0476974.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26047..26919
                     /gene="rsmI"
                     /locus_tag="ACG90W_000583"
     CDS             26047..26919
                     /gene="rsmI"
                     /locus_tag="ACG90W_000583"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0476975.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27028..28149
                     /gene="dinB"
                     /locus_tag="ACG90W_000584"
     CDS             27028..28149
                     /gene="dinB"
                     /locus_tag="ACG90W_000584"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0476976.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28203..28805)
                     /gene="nrdG"
                     /locus_tag="ACG90W_000585"
     CDS             complement(28203..28805)
                     /gene="nrdG"
                     /locus_tag="ACG90W_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0476977.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29120..31309)
                     /gene="nrdD"
                     /locus_tag="ACG90W_000586"
     CDS             complement(29120..31309)
                     /gene="nrdD"
                     /locus_tag="ACG90W_000586"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0476978.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31635..32402
                     /locus_tag="ACG90W_000587"
     CDS             31635..32402
                     /locus_tag="ACG90W_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0476979.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32392..34434
                     /locus_tag="ACG90W_000588"
     CDS             32392..34434
                     /locus_tag="ACG90W_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0476980.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34715..34864
                     /locus_tag="ACG90W_000589"
     CDS             34715..34864
                     /locus_tag="ACG90W_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0476981.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34875..36389
                     /gene="dltA"
                     /locus_tag="ACG90W_000590"
     CDS             34875..36389
                     /gene="dltA"
                     /locus_tag="ACG90W_000590"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0476982.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36386..37591
                     /gene="dltB"
                     /locus_tag="ACG90W_000591"
     CDS             36386..37591
                     /gene="dltB"
                     /locus_tag="ACG90W_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0476983.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37636..37869
                     /gene="dltC"
                     /locus_tag="ACG90W_000592"
     CDS             37636..37869
                     /gene="dltC"
                     /locus_tag="ACG90W_000592"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0476984.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37872..39140
                     /gene="dltD"
                     /locus_tag="ACG90W_000593"
     CDS             37872..39140
                     /gene="dltD"
                     /locus_tag="ACG90W_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0476985.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39281..40363
                     /locus_tag="ACG90W_000594"
     CDS             39281..40363
                     /locus_tag="ACG90W_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0476986.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40363..41166
                     /locus_tag="ACG90W_000595"
     CDS             40363..41166
                     /locus_tag="ACG90W_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0476987.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41236..41976
                     /locus_tag="ACG90W_000596"
     CDS             41236..41976
                     /locus_tag="ACG90W_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0476988.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            41987..42991
                     /locus_tag="ACG90W_000597"
     CDS             41987..42991
                     /locus_tag="ACG90W_000597"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0476989.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43036..>44854)
                     /locus_tag="ACG90W_000598"
     CDS             complement(43036..>44854)
                     /locus_tag="ACG90W_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0476990.1"
                     /translation="KKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEI
                     KDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWIT
                     VYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKFYL
                     GTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALA
                     LGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQ
                     LLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWI
                     VLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDF
                     LKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEA
                     KNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHAL
                     EHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERF
                     DLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14407 a         7829 c         9339 g        13279 t
ORIGIN      
        1 atctggtacg aaaatcaact agataccttt tttgtgttct tatttttttg ttattttata
       61 aaaaatagag atctcatgtt agaaaaccta acatatgcag ttgacaatat tttttttaat
      121 tggtacactt tagccaactt ataaaacgaa aggggacgtc tgatgggaga aaaggaattg
      181 cgccgatcta tgtccgtatt tccaattggt acagttatga agctgactga tttgtctgct
      241 cggcaaattc gctattatga agaacaagat ttagttcatc cggaacgaag cgaaggaaac
      301 cgacgtatgt attcgctgaa tgatattgat atgttgcttg agattaaaga ctatctttca
      361 gagggattga atatggctgg gatcaaacgt gtatacgaaa tggcacaggc gaaaaaacaa
      421 gaagcaaaaa acaagaagcc gttgacagat catgacgttc gacaaatttt tcgtgatgag
      481 attttggcgc aaggtgggtt aagcaaaaca ggtcattatc agtcgcaagg attgcaaaga
      541 caattctttg actgaataaa ttaaaaaagg aagagggata ccatggtaaa gaaacagata
      601 acaggtgaag aaatcaaacg aatcattgag gaagaaaacg tacgattttt acgattgatg
      661 ttcacagaca tcttaggtac gatcaaaaat gtagaagttc cagtgagcca gatcgacaaa
      721 gtattagaaa ataaaatgat gtttgacggt tcttcgattg aaggatttgt tcgaattgaa
      781 gaaagcgata tgtatttgta tccagatctc tccacttgga tgatcttccc gtgggaaagt
      841 gctcatggaa aagttgctcg tttaatttgt gatatctata atccggatgg gacaccattt
      901 gccggcgatc ctcgcgggaa tttgaaacga gcattgaaag atatgcgcga ccttggtttt
      961 acctctttca atttaggacc tgaaccagaa ttcttcttat ttaaattaga cgaagacggt
     1021 gggatcacaa cgaccttgaa tgataaaggt ggttattttg attttgcacc aacagattta
     1081 ggtgaaaatt gccgccgtga catcgtgttg gaattagaaa gtctaggctt tgaagtggaa
     1141 gcttcacatc atgaagtagc gccaggacag catgaaattg actttaaata tgcagatgtg
     1201 gtagatgctt gtgataatat ccaaaccttc aagctcgttg tcaaaacgat tgcacgcaag
     1261 catggacttc acgcgacttt catgccgaaa ccgttatttg ggatcaatgg ttctggtatg
     1321 cattgcaata tgtctttgtt caatgatgaa ggaaatgttt tctatgataa agacggtgaa
     1381 ttggaactaa gtgaaacagc ttatcatttc ttaggtggtt tgctgaaaca tgctcgtgcc
     1441 tacacagccg tttgcaatcc aacagtcaat tcttataaac gtctcgttcc aggttatgaa
     1501 gcgcctgttt atgtcgcatg gagtggccgt aatcgttcgc cattagtacg tgtaccagaa
     1561 tctcgcggtt tatccacacg tttggaattg cggtcagttg atccatctgc aaacccatac
     1621 ttggcaatgg cagtattgtt gcaagcagga ctagacggta tccgtaatca gatcacaccg
     1681 ccagcagctg ttgaccgtaa catttatgta atggatgagg aagaaagaga agctgcacat
     1741 atccaggact taccttcaac tatccataat gctatcaaag aattgcgtaa ggataatgta
     1801 atgattgacg cattaggaca acatatcttc tcaaattttg ttgaagcaaa acgtctcgaa
     1861 tgggcggcat tcagacaaac tgtttccgag tgggaaagag aacaatatct agaactttat
     1921 taaaacagga ataccgtgaa ttagaggctg tgaaatcagc gttttgacct gagtattaga
     1981 gaaactaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     2041 agctgattga acaccgttta gtggagaggt tgtgaaaaag ctgccctgca acaagtaata
     2101 agaatagcca agcaaaaaca gcttttcttg tttttgaatt ggctgaactt attaccgcag
     2161 atgcaagctt ttgaacaccg tttaccaaga ggttttgaaa aagacgttta gtttcgagta
     2221 ttaagaaaaa ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg
     2281 agaaactggc ttttgaatac cgtttatcca gaggttgtga gcctaccatt taactccgaa
     2341 tcacaagaaa taatttcaaa aacagcttct catgtttgat ggatattgtg acttgtgacc
     2401 gaggagttgg caggagaaca ccgtttattt agaaataagg aggcacgact tttgtcgcat
     2461 ctccttattt tttacttatg tgattcgtta ccttcaatga atccctacaa ggcacttttg
     2521 ttgaaaaaaa ggtcatcttt gaaaaaatta gtggaattgt tttgtttttg atattattta
     2581 atagaaaaga tatgccaaaa atccgagaac tgtggactac gttttttacg taaaggaggt
     2641 aaataagggt gaaagaaaaa gattgtttct acgataagct atataaactg ttgctgcaag
     2701 taggaaaaag tagttctttg ttaggtggga tattatctat cataatgttt gttttatttt
     2761 tttatctatt atttcactta aaatttttca gtaacgattt tctgatttgc ttaggtttaa
     2821 cgttagctat gtttatatca agcttattat gtgggttaaa ttttttttgc actcataaag
     2881 gacttaagaa ataatttctc aaaggatatc gaaaaagaaa aaattgtaag cggatagtat
     2941 tcatttgagt acttgacttt cccttagtca gcttgtatta tacaccttgg gcaccctttt
     3001 agagggtcag aagtttacag aaatttcggc tccctattct tattgaagaa tagggagctt
     3061 tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg aacttatgac
     3121 aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga ttgttgcagc
     3181 ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa aatttgatcc
     3241 atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag tattcgtaac
     3301 agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta ttgacatcaa
     3361 tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt tgcgtaaaga
     3421 acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta caaatgagat
     3481 caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca ttacagaagt
     3541 agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc gtcaaatgaa
     3601 agcagatgtc ggtacagata atgttatgta tatccataca acattgattc cttacttgaa
     3661 agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc tacgtggttt
     3721 aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc aaagcgtgaa
     3781 aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt cgccagatgt
     3841 agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc aaattgtttg
     3901 cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag aattagaaga
     3961 acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat atgtcgagtt
     4021 accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg cctataattc
     4081 agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg tagaagaacg
     4141 tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg gagtagaagg
     4201 gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag gtatttgtct
     4261 tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag aagatgcgtc
     4321 atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg ctgatcagga
     4381 aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta aaatcaaaaa
     4441 aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac gtcaccgtca
     4501 ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc tagtcttttc
     4561 aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga aaaaattctt
     4621 tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc aaaaattaat
     4681 caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt tgaacttgat
     4741 agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt ttctcttttt
     4801 ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt tcgattaaag
     4861 cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt ttcattgatt
     4921 ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga attgtcttaa
     4981 caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat caggagctga
     5041 atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca caaacaactt
     5101 ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag tacttatcca
     5161 aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag atttaatcac
     5221 tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag accatggtga
     5281 ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt tcgatggttc
     5341 acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa aagcacatgc
     5401 aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag acggaatcat
     5461 cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta caggtatcga
     5521 ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact ggaaaggatt
     5581 agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc ctttagtatt
     5641 acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa tgggtatctc
     5701 aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc gtaaatatat
     5761 cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat tagctccagg
     5821 taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt cagctaacaa
     5881 agcgtaagca tccacataac agatacgata tataggaatg gagaacgact tttgaagtcg
     5941 ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt ttatctatga
     6001 taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca gatgaaaaaa
     6061 atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg tgcaaaaaat
     6121 agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat cttggaaggt
     6181 gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat gggcgcaact
     6241 gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc gattccgatg
     6301 ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac actgcttggt
     6361 aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc tagaccgatt
     6421 gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga acatggtgcc
     6481 atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat ggacgtggtt
     6541 tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg acagacagta
     6601 attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact gaataatatg
     6661 ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt cgaaacatta
     6721 catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta tctagcatta
     6781 gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca tttggaaagc
     6841 tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga tgaaatcgaa
     6901 gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta tccaggattt
     6961 gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg cacttcagaa
     7021 atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc acgaatgggc
     7081 gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa acttcacggc
     7141 acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc tggactattg
     7201 gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta tgatcatatt
     7261 atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt gggagctgaa
     7321 tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa gtttatagtt
     7381 atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa ttatctctag
     7441 ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga atattggata
     7501 tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca agtgcagaag
     7561 atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg gacaaagtgg
     7621 caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg cacgtggaaa
     7681 gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca gccttaacac
     7741 ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta tctacccgaa
     7801 tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc gcaccgccaa
     7861 aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa aactatccgg
     7921 aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg gatctagaac
     7981 gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa aaccatactc
     8041 gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa cgagatgtcg
     8101 ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc gttccaccaa
     8161 gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct aagaaatttt
     8221 tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg accgctttag
     8281 tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga actggaaatc
     8341 tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc atcgatatca
     8401 aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta gaagaaattt
     8461 ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa ctgatccgct
     8521 ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat gtctcctttg
     8581 gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg aaacatgtta
     8641 agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag cagggcaaag
     8701 gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag tattcatgga
     8761 ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag aaacagttga
     8821 atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg actcacatcc
     8881 attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc gtttcaacaa
     8941 aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat aactcaacgt
     9001 ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa tttaaaactt
     9061 cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa actagtatat
     9121 agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga ggggttattt
     9181 cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa ggaaggcttt
     9241 ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta atgacttata
     9301 cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt acagctagcg
     9361 atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg agaggttttt
     9421 tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat cgatagattc
     9481 aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata aggtaagaaa
     9541 tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca caggaataga
     9601 agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag cactgtgttt
     9661 ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa ttgaatatat
     9721 ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc tcagcacact
     9781 ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca ctacgccagc
     9841 agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc cgagtatcga
     9901 tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc ttgcccgact
     9961 ggaattgctc atacttatat gtcggcagaa aaattagaag taacagcaag aaatctaaac
    10021 catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc tgaacaagaa
    10081 atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt ggaccgattt
    10141 gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc gcaagtagta
    10201 atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa agaaccagct
    10261 gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa ttacatgctt
    10321 ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg gattacagcg
    10381 gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag gatcggagga
    10441 gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac atcgattgct
    10501 ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac aggtggttca
    10561 ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga ttttctggca
    10621 aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt cgtccccttt
    10681 ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc gatcacatgg
    10741 gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg gctattgttc
    10801 ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa taaatcgact
    10861 tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc tgcttgtatg
    10921 gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt caaacataaa
    10981 tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc ttcgtatatt
    11041 acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc tagtttgatg
    11101 acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc aatggcacct
    11161 catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc tatgtatgtc
    11221 ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt gaaaaaaaca
    11281 gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa tgaggtggaa
    11341 gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat caatttgcag
    11401 taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa gaagcagaga
    11461 gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat ttaagtgatg
    11521 agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc gttatccact
    11581 tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc tattcttccg
    11641 tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg aaaaaagtgg
    11701 ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg attgggaata
    11761 atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat caagcgaaga
    11821 tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca gcgcatggct
    11881 tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa ttgaaccaac
    11941 aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca gaagtcagcg
    12001 aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa agcgtctttt
    12061 tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg aatgcaattt
    12121 atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa attttgaaca
    12181 cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg gtatgacaat
    12241 agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt cgaaaaattt
    12301 cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg ttactatata
    12361 aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg agattgtgaa
    12421 ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag taaattatag
    12481 cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga tccgtagagt
    12541 tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc tgatgaagat
    12601 caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt cttctttgat
    12661 tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact tagtcagaaa
    12721 cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag ctgctatgtt
    12781 gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc cgaaacgagc
    12841 aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg atttagcgat
    12901 aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg aaggagaggc
    12961 aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag ttacacagca
    13021 aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg attatctggt
    13081 gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc atatcgaatg
    13141 tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt tagataggac
    13201 cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta tcttgttaga
    13261 taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt gaaggcgttg
    13321 gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg tttatacggt
    13381 gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat ctatgtgcta
    13441 ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc agatgctggt
    13501 tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt cgatatttgg
    13561 atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt ccacaagtgg
    13621 ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa cagttaaact
    13681 attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag aataatcata
    13741 aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt tttttttatc
    13801 tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct ctgcttcatg
    13861 actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa tgactccggc
    13921 aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca aataaggacc
    13981 aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac tgattcgatg
    14041 ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac cataacctag
    14101 gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac tcagtaaaaa
    14161 taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc ctttgacatc
    14221 gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata cgacgaagaa
    14281 aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa atgataaaac
    14341 acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg ctttcttttc
    14401 aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag ataatggaat
    14461 attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta caattgaaaa
    14521 ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa atggcccaac
    14581 agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt ttttagatgc
    14641 agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt agccgaatcc
    14701 attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat atgctaatgt
    14761 tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa atagttctaa
    14821 ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc cagaagcaaa
    14881 acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca tgatgaccat
    14941 caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa taaaatattt
    15001 attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat atgcttttaa
    15061 tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa caagttggcg
    15121 aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt cgtttgttgg
    15181 attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat ttaaaaagac
    15241 taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat taatgattgt
    15301 gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact tctgcagtat
    15361 aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact taacaaatgt
    15421 taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg tatgatcttt
    15481 ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa ggaatatttg
    15541 gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac attcgaaggg
    15601 atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag tgttatttca
    15661 aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt ctctctttta
    15721 aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga atcagaatat
    15781 gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca agacccaaag
    15841 ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat gaaaaaaatg
    15901 cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta cgaattatat
    15961 gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat cacgaatgaa
    16021 gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat tttaaaacaa
    16081 ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt gaatatggac
    16141 ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca gcgttttgac
    16201 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta
    16261 ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac
    16321 tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact
    16381 tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa aagctgttct
    16441 gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa cttggctgga
    16501 cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag gggctgtgac
    16561 acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt agaaacttct
    16621 gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc caccgccgct
    16681 tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg tcagacgatc
    16741 ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt agatgcctga
    16801 tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga taagagccaa
    16861 aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt
    16921 ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg ttgctgattt
    16981 agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat cataaacatc
    17041 atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac cagaagtcga
    17101 aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt gatcatttcc
    17161 gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat tagatgtagt
    17221 cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct gttgttccaa
    17281 agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag ctgtatctgc
    17341 atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa aaaatttttt
    17401 catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa aatcccaaga
    17461 gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt tcctgtttgt
    17521 ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata agtaacaagc
    17581 cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga agttcgcaca
    17641 ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga atagtctttt
    17701 aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt ttctgcttgg
    17761 aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa aggttgcacg
    17821 ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag ttctttaaaa
    17881 gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct
    17941 atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac ttcagcatct
    18001 gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt attgaaaaaa
    18061 gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat tgcttcttct
    18121 ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa acgtcccgat
    18181 agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg
    18241 atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt atctgcagat
    18301 tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc
    18361 atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct
    18421 ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg gctgacttct
    18481 tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt gggatcaaaa
    18541 gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag ctgcacacct
    18601 ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt tggacagaat
    18661 ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg catttgtagc
    18721 ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa ttttccggaa
    18781 ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc agtttgttga
    18841 ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc ttgagaaaga
    18901 taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat tgctcctgct
    18961 tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta gccttcacgt
    19021 acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt cacagaatca
    19081 tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg taattcacgc
    19141 caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt agatggtaca
    19201 taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag cgctgttagg
    19261 aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt gtttttaggg
    19321 attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt gccatgtgcc
    19381 cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg
    19441 aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt ctgcggtgtg
    19501 acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag tgttccattg
    19561 aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc ggtataatcg
    19621 atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg gacgactgtt
    19681 ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc atctttgcgg
    19741 actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc gatgacttct
    19801 agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat aagacccata
    19861 aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta ttataccata
    19921 ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg cgtattgttt
    19981 aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac gtataataag
    20041 agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact ttatcgagtc
    20101 tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa
    20161 aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc cagaggaacc
    20221 ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa cagtaaagac
    20281 ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat
    20341 gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt tatccgtgat
    20401 cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga tgaggtacat
    20461 atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc aaaagaaagt
    20521 gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat catttcacgg
    20581 actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca tcttgaattt
    20641 atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat tgcccgttct
    20701 gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc gtttagtgaa
    20761 ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga cgaaatgatg
    20821 gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga aacattgaac
    20881 caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt acaatactgc
    20941 cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc gagtacgatc
    21001 agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt ttcaatgata
    21061 aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc tatttatttg
    21121 gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc agcagatgta
    21181 gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga actaagaaac
    21241 gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca gccggtacag
    21301 caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg agtatataaa
    21361 gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg ggaagacttg
    21421 ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc agttgcagct
    21481 ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag agcagcaaat
    21541 gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa agattatgcg
    21601 cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa ttatttgacg
    21661 caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga aggagaaatc
    21721 gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa agcagaggaa
    21781 ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt agataattat
    21841 gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga
    21901 aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag ccaccaatga
    21961 attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga
    22021 agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga
    22081 tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg caaaaggttt
    22141 gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag aaccaattgc
    22201 gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag cgacaagact
    22261 cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta aatctttgtt
    22321 aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag aagaagatcc
    22381 ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc
    22441 gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc atgttctcca
    22501 tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt cttctctgtt
    22561 gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg caacaacaga
    22621 gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta tcaaagtgac
    22681 tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg aagttacttt
    22741 attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt tttttcagat
    22801 acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat ttattacaat
    22861 cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc ctcttttgaa
    22921 agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc cgattgctga
    22981 agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac gtacggaagc
    23041 actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc ctgctctggc
    23101 aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc
    23161 aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta cagaaggtac
    23221 tacgccggat tttacccttt atcttgacgt agattcagac accggattaa gaagaatcaa
    23281 gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat tccaccagcg
    23341 tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc acaaagttga
    23401 tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta ttgaacagta
    23461 tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt agcgattatc
    23521 caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat ccgtgccaca
    23581 aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat cgtgggtatt
    23641 gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga atcaagaaag
    23701 caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt cccttatcca
    23761 gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg attccatcaa
    23821 ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt
    23881 gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt atctttttga
    23941 aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc acctgttctg
    24001 tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa
    24061 gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca aagtcgatca
    24121 gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa aaaaagtttt
    24181 tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt tgaaattctt
    24241 ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag gaagaattct
    24301 ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga aagaagcact
    24361 gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt tggctttctt
    24421 aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta atgatgctaa
    24481 ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag cctttatcta
    24541 tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta caggattatc
    24601 tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg
    24661 caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc agaaactacg
    24721 cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga ttttttagct
    24781 tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg tgtccgatat
    24841 agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt ttatagtgac
    24901 aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct tcctaaaaaa
    24961 gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa agcgtcacaa
    25021 gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca
    25081 aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt
    25141 gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg
    25201 gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc
    25261 gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct
    25321 tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg
    25381 aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc
    25441 gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct
    25501 gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg
    25561 actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta
    25621 ggtgagatga atggataaga gatcattata cgacggattg aatcaattag aatcagagtt
    25681 gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa
    25741 aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg aattgaacca
    25801 agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct
    25861 tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga
    25921 caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc
    25981 aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg
    26041 tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt
    26101 gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa
    26161 gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt
    26221 gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa
    26281 ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct
    26341 tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg
    26401 atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct
    26461 caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg
    26521 aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta tcggatcact
    26581 gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa
    26641 ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc
    26701 gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg
    26761 ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc
    26821 gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa
    26881 aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa
    26941 tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg
    27001 ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat
    27061 acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga
    27121 gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt
    27181 gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca
    27241 atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat
    27301 cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt
    27361 gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc
    27421 aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta
    27481 acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa
    27541 aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg
    27601 ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg
    27661 atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga
    27721 aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt
    27781 acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa
    27841 gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga
    27901 gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg
    27961 accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca
    28021 agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact
    28081 ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa
    28141 gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt
    28201 ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt
    28261 tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg
    28321 ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca
    28381 aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca
    28441 aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt
    28501 cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat
    28561 ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat
    28621 ggtttgccat atcgaaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag
    28681 cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg
    28741 tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt
    28801 ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta
    28861 ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt
    28921 attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact
    28981 tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc
    29041 ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca
    29101 ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc
    29161 ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta
    29221 catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg
    29281 gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa
    29341 gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt
    29401 ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt ggataatcct
    29461 tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt
    29521 ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac
    29581 ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat
    29641 attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag
    29701 cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca
    29761 atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat
    29821 cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc gcatttgctg
    29881 gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc
    29941 ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg
    30001 gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt
    30061 cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta
    30121 attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca
    30181 atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca
    30241 aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc
    30301 gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc
    30361 ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca
    30421 aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc
    30481 gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg
    30541 caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg
    30601 ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt
    30661 cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc
    30721 agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt
    30781 tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat tttccaacga
    30841 tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt
    30901 tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt
    30961 tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt
    31021 cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt
    31081 cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat
    31141 aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga
    31201 tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg
    31261 tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact
    31321 cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg
    31381 gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat
    31441 tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttaatt
    31501 ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac
    31561 ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa
    31621 gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca
    31681 gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt
    31741 ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca
    31801 cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga
    31861 aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca
    31921 atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga
    31981 tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag
    32041 aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg
    32101 ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg
    32161 actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag
    32221 caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat
    32281 ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct
    32341 ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat
    32401 aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt
    32461 ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta
    32521 gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc
    32581 attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg
    32641 caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata
    32701 tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt
    32761 attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact
    32821 agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc
    32881 cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt
    32941 tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta
    33001 ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg
    33061 gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt cttttttcct
    33121 tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta tacatttcgg
    33181 ttatttttga tcatattgag taaatggaag atttattact atggtatcaa ttttttgata
    33241 atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct gattacagtc
    33301 attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac tattttatcc
    33361 taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac agagaaaaaa
    33421 atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac cttgcattat
    33481 aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca aactgctaat
    33541 ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt gccgaacatt
    33601 tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct ttttcggaat
    33661 atttcattat atggatctag tattggactg ccagataatc aagaattgaa gattcaacaa
    33721 atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt actgattgta
    33781 gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa tgtagatgta
    33841 tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc gataaagtgg
    33901 ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg agaaattagc
    33961 acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga aagctctcgt
    34021 ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat
    34081 atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag tattttgaca
    34141 gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt gactaagctg
    34201 gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact taccttcttt
    34261 ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt cactcagtat
    34321 ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat ccttatctat
    34381 cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga atgatatacg
    34441 gaataagaca agataataaa tagaactaaa ttttgaatac catttatttg gaataagagg
    34501 ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa gtacgagaaa
    34561 ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg tgacaaaact
    34621 gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca aaattagtta
    34681 cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa
    34741 attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag tactgctgtt
    34801 cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct ataacgaatt
    34861 ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat tggggaataa
    34921 atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt gaattgaaac
    34981 aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat ggaccaattg
    35041 tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg
    35101 gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta tcgatctttc
    35161 gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat attccttcgg
    35221 ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta ggattttatc
    35281 ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct ggtactactg
    35341 gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt caatggacga
    35401 tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca ttttcttttg
    35461 atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg aagccgttaa
    35521 ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc ttgaagttga
    35581 atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct acattcaatg
    35641 gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg acgaaaaaaa
    35701 ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact tacggtccaa
    35761 cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta gatagttatg
    35821 accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc caagaaggta
    35881 aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc gtatcaaaag
    35941 gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa
    36001 gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt tatgaaggac
    36061 gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat atcgaacatt
    36121 atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa
    36181 aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag ccggattttc
    36241 agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat atgattcctc
    36301 agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa
    36361 aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt atgcagagcc
    36421 gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt tattgatatg
    36481 ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat ttgtcagctt
    36541 tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt ggcagacgat
    36601 acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat ggttttttgc
    36661 cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt tttttgaaca
    36721 tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta aatctgtaca
    36781 aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc gttatatcca
    36841 gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata ggagatttga
    36901 aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt
    36961 ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt ttggtcaagt
    37021 actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat gggcattagt
    37081 aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct atagcttatt
    37141 tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca ataaaccttt
    37201 tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt ccttttggtt
    37261 ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat tttttaaaag
    37321 caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg gtatatggca
    37381 tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga tctgtctgac
    37441 agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta acaaatttac
    37501 gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc tgattttttc
    37561 aggattttta gataaacttt tttttagcta acgaatagtg acatattaat ttatataaca
    37621 aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat aacaggaaca
    37681 gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct tgattcttta
    37741 gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt ccctgtatct
    37801 gaattcgagc gagaggattg gtgcacaccg aataaaatca ttcaacaagc aactgcattg
    37861 aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt ttagctgttg
    37921 gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat ccagaaacga
    37981 ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt
    38041 atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta agcagaatca
    38101 gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt ccatttttgc
    38161 tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct gcaggagatg
    38221 ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt aaaaatggtg
    38281 taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac tggttgtttt
    38341 caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta actttctcaa
    38401 aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac
    38461 ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag agagaggatg
    38521 aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac
    38581 gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa attggagaac
    38641 gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat cgtatccgag
    38701 cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt tcgccagaat
    38761 tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa gtattgttca
    38821 tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa gaaatgttac
    38881 aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat cgaatagctg
    38941 attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat ctaggctgga
    39001 aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat catattactg
    39061 ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat caaataccag
    39121 ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt tctttatttt
    39181 tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat cataagcaat
    39241 ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa aagaagaaca
    39301 gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag aggtaagaga
    39361 agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg gtttaggcag
    39421 tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg gacatatgga
    39481 cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat tccaaactct
    39541 tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg
    39601 gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac ctcaagtaag
    39661 aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa gccaagaaga
    39721 agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg aatacaaacg
    39781 aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg ggacagctgt
    39841 ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt tattcgcagg
    39901 aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac accttgtaaa
    39961 tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag gaatgacacc
    40021 aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg atgcatctat
    40081 gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa tggatattcc
    40141 attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc atttgacaag
    40201 agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt cccatacatc
    40261 agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat tagtgaaacg
    40321 gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa tagaatacct
    40381 atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg aatcaatact
    40441 tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt tgacggtttc
    40501 agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt cgataattta
    40561 cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt acggggtggc
    40621 agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg ttcgatactt
    40681 ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat ctttctgccc
    40741 atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc aaatgcggta
    40801 cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc gatgataggg
    40861 atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca atttgctgca
    40921 tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata tggctggttt
    40981 ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat cggctggtat
    41041 ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta tgtgaatgtg
    41101 tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga cgcctatttt
    41161 acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg ataaatcttt
    41221 gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta tccttataaa
    41281 aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca
    41341 ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct agatgctgtg
    41401 atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca gtattactgg
    41461 caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca cacaaaagat
    41521 tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc ttattttgaa
    41581 gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat ccatccagta
    41641 gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt atttacaggt
    41701 gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt gtttctagca
    41761 gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc aaaagaagct
    41821 ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct acctcagttt
    41881 ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg gattgatata
    41941 gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga tttttgtacc
    42001 aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc tgcaagaaat
    42061 gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca tcatcggcag
    42121 aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg gcattcatgt
    42181 tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc tgaactttag
    42241 ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa cacagccaat
    42301 catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct
    42361 agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt
    42421 tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg cattaaaagt
    42481 aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg taacgaatat
    42541 caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat
    42601 cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg aattgactga
    42661 tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt gggattggaa
    42721 ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat
    42781 tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt
    42841 cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga aatatgataa
    42901 agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg gtgcgataga
    42961 accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat ccacatatat
    43021 ggattccgct attttttata caggattcgg gtaatttcga gagagttgaa cagtgatccg
    43081 atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg taacgatccg ctacgtcttt
    43141 catcatcatt aagtcaaaac gttcctgatc atctaattct tctgtgatgc ctcgactaaa
    43201 taggtttgta tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt cctcttgttc
    43261 gtgtatcgac aaataaattt ttcgatacag tggattttcg atggcatgag tttctctaaa
    43321 ggaatgctct aaagcgtgga tcaaaagttg agccagatcg tcatcgttga taggaagttc
    43381 ttcgggaaga tctgcttgga aggttatctg aatattccgt ttggcagcta acttctggta
    43441 acgcgctaag ataagatttg tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt
    43501 ttttgcttct tctaatatct tttccacata gtttttagct tttgtttggt ccgtatgcat
    43561 taaatcttgc agattctcca tctcttggac aaattccagg cgaagtgtat ttacttcttg
    43621 gatatgctgt cgaagctgct cataatgttg ctccaacaaa tcatttttga tttttaagaa
    43681 atctacttgt ctccgatctt tgtcggtatt taagatgtga tcgattagat tgtagctgat
    43741 aataacgatt agaatgataa tatataaaat cgtagaccaa ttgatcgttg tttgatagat
    43801 acctaatgcc gtttggatgt aaatcaagca atggataatg gctaccaaga cgatccaagg
    43861 tgtacaagct acataaaaac ggttattctt atatgcttcg ccaatgcatg caagtgaagt
    43921 gactaaagtg cttataacac tagcaatatt agcaatcggc attaattcag gaagttctgc
    43981 aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa
    44041 aattccgtac cattttttat agtaaaacat tcggctccaa tagtagcaga tgatgaaagc
    44101 tgacgttaag ataataaata aatgagataa aaaagaatga acgaatggtt caaaaagaat
    44161 accacttaaa atatcttctg atacagcttc aaatcctaat gccaaagcaa agcaacttaa
    44221 gagaataagt gagtattcaa gacgtttttg tttatacatc aagtagatcg tatagaaaaa
    44281 ggtccctata gcgataaaaa cagcgattat catcgtcaat acttgcggca ctgattgtga
    44341 ataaataaaa ctaatcaatg gtccagttgt tcctagataa aatttcggtg gcagtccagc
    44401 atagttctga taaggagatg agactgtaag ggtcaatttt tttccgatat aatttgctgg
    44461 taaatctatc acagccaatg agagtccggg attgctttct tcctgataat ctttccgttg
    44521 gtataaaatc ttttcgccta gatatacagt aatccattga tggtttcctt tgattagtaa
    44581 atttgcttcg tccaacggtt tttcaagtgt cgtttccata atgaatgttt cgtctttatc
    44641 cacggtagga atcgtacgag tgtacttgct gtcaaactta aaatcaggat catcttttgt
    44701 atagaaaacc cacgaatctt ttatttcttt catttgattg ctgtccgcag gagaaaaaat
    44761 aaaataaaag aaataagcaa ttcctgttaa aaataacaat gataatataa tgattatgct
    44821 ccactttata cgtaaataaa aagattcttt ttta
//
LOCUS       DAZKOK010000012        38649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000012 DAZKOK010000000
VERSION     DAZKOK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.012"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..1204)
                     /locus_tag="ACG90W_000599"
     CDS             complement(65..1204)
                     /locus_tag="ACG90W_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0476991.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1513..2226)
                     /locus_tag="ACG90W_000600"
     CDS             complement(1513..2226)
                     /locus_tag="ACG90W_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0476992.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2480..3181)
                     /gene="nagB"
                     /locus_tag="ACG90W_000601"
     CDS             complement(2480..3181)
                     /gene="nagB"
                     /locus_tag="ACG90W_000601"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0476993.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3415..4947
                     /locus_tag="ACG90W_000602"
     CDS             3415..4947
                     /locus_tag="ACG90W_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0476994.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(5138..6340)
                     /locus_tag="ACG90W_000603"
     CDS             complement(5138..6340)
                     /locus_tag="ACG90W_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0476995.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(6422..7111)
                     /locus_tag="ACG90W_000604"
     CDS             complement(6422..7111)
                     /locus_tag="ACG90W_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0476996.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(7217..7585)
                     /gene="rplL"
                     /locus_tag="ACG90W_000605"
     CDS             complement(7217..7585)
                     /gene="rplL"
                     /locus_tag="ACG90W_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0476997.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(7638..8141)
                     /gene="rplJ"
                     /locus_tag="ACG90W_000606"
     CDS             complement(7638..8141)
                     /gene="rplJ"
                     /locus_tag="ACG90W_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0476998.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(8345..8472)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(8551..9240)
                     /gene="rplA"
                     /locus_tag="ACG90W_000607"
     CDS             complement(8551..9240)
                     /gene="rplA"
                     /locus_tag="ACG90W_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0476999.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(9344..9766)
                     /gene="rplK"
                     /locus_tag="ACG90W_000608"
     CDS             complement(9344..9766)
                     /gene="rplK"
                     /locus_tag="ACG90W_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0477000.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            10109..10777
                     /gene="sdaAB"
                     /locus_tag="ACG90W_000609"
     CDS             10109..10777
                     /gene="sdaAB"
                     /locus_tag="ACG90W_000609"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0477001.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            10790..11662
                     /gene="sdaAA"
                     /locus_tag="ACG90W_000610"
     CDS             10790..11662
                     /gene="sdaAA"
                     /locus_tag="ACG90W_000610"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0477002.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(11753..12853)
                     /locus_tag="ACG90W_000611"
                     /pseudo
     CDS             complement(11753..12853)
                     /locus_tag="ACG90W_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(12945..13832)
                     /locus_tag="ACG90W_000612"
     CDS             complement(12945..13832)
                     /locus_tag="ACG90W_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0477003.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(13909..14454)
                     /gene="nusG"
                     /locus_tag="ACG90W_000613"
     CDS             complement(13909..14454)
                     /gene="nusG"
                     /locus_tag="ACG90W_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0477004.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(14555..14725)
                     /gene="secE"
                     /locus_tag="ACG90W_000614"
     CDS             complement(14555..14725)
                     /gene="secE"
                     /locus_tag="ACG90W_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0477005.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(14749..14901)
                     /gene="rpmG"
                     /locus_tag="ACG90W_000615"
     CDS             complement(14749..14901)
                     /gene="rpmG"
                     /locus_tag="ACG90W_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0477006.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            15063..15707
                     /gene="cbpA"
                     /locus_tag="ACG90W_000616"
     CDS             15063..15707
                     /gene="cbpA"
                     /locus_tag="ACG90W_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0477007.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(15763..16266)
                     /locus_tag="ACG90W_000617"
     CDS             complement(15763..16266)
                     /locus_tag="ACG90W_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0477008.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(16279..16325)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            16863..17828
                     /locus_tag="ACG90W_000618"
     CDS             16863..17828
                     /locus_tag="ACG90W_000618"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0477009.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(17927..19099)
                     /locus_tag="ACG90W_000619"
     CDS             complement(17927..19099)
                     /locus_tag="ACG90W_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477010.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(19086..20231)
                     /locus_tag="ACG90W_000620"
     CDS             complement(19086..20231)
                     /locus_tag="ACG90W_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477011.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(20245..21169)
                     /locus_tag="ACG90W_000621"
                     /pseudo
     CDS             complement(20245..21169)
                     /locus_tag="ACG90W_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            21286..22365
                     /locus_tag="ACG90W_000622"
     CDS             21286..22365
                     /locus_tag="ACG90W_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0477012.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            22362..22990
                     /locus_tag="ACG90W_000623"
                     /pseudo
     CDS             22362..22990
                     /locus_tag="ACG90W_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(23100..23327)
                     /locus_tag="ACG90W_000624"
     CDS             complement(23100..23327)
                     /locus_tag="ACG90W_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0477013.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(23430..26018)
                     /locus_tag="ACG90W_000625"
     CDS             complement(23430..26018)
                     /locus_tag="ACG90W_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0477014.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(26038..26697)
                     /locus_tag="ACG90W_000626"
     CDS             complement(26038..26697)
                     /locus_tag="ACG90W_000626"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0477015.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(26746..27249)
                     /gene="trmL"
                     /locus_tag="ACG90W_000627"
     CDS             complement(26746..27249)
                     /gene="trmL"
                     /locus_tag="ACG90W_000627"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0477016.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(27470..28336)
                     /locus_tag="ACG90W_000628"
     CDS             complement(27470..28336)
                     /locus_tag="ACG90W_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0477017.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            28445..29086
                     /locus_tag="ACG90W_000629"
     CDS             28445..29086
                     /locus_tag="ACG90W_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0477018.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(29148..30509)
                     /gene="mgtE"
                     /locus_tag="ACG90W_000630"
     CDS             complement(29148..30509)
                     /gene="mgtE"
                     /locus_tag="ACG90W_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0477019.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(30527..31426)
                     /locus_tag="ACG90W_000631"
     CDS             complement(30527..31426)
                     /locus_tag="ACG90W_000631"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0477020.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(31428..32225)
                     /locus_tag="ACG90W_000632"
     CDS             complement(31428..32225)
                     /locus_tag="ACG90W_000632"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0477021.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(32203..32907)
                     /locus_tag="ACG90W_000633"
     CDS             complement(32203..32907)
                     /locus_tag="ACG90W_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0477022.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            33038..33614
                     /locus_tag="ACG90W_000634"
                     /pseudo
     CDS             33038..33614
                     /locus_tag="ACG90W_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            33732..34409
                     /locus_tag="ACG90W_000635"
     CDS             33732..34409
                     /locus_tag="ACG90W_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0477023.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(34488..36296)
                     /gene="pepF"
                     /locus_tag="ACG90W_000636"
     CDS             complement(34488..36296)
                     /gene="pepF"
                     /locus_tag="ACG90W_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0477024.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(36472..37470)
                     /locus_tag="ACG90W_000637"
     CDS             complement(36472..37470)
                     /locus_tag="ACG90W_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0477025.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(37696..38349)
                     /locus_tag="ACG90W_000638"
     CDS             complement(37696..38349)
                     /locus_tag="ACG90W_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0477026.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        11547 a         8141 c         6785 g        12176 t
ORIGIN      
        1 ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt
       61 atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt
      121 ccattcttct caataaatag attttccgga tatttaatat tatcgtctta atcactgcat
      181 agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata
      241 ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca
      301 aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg
      361 gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg
      421 ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata
      481 ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga
      541 tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg
      601 ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata
      661 gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt
      721 ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat
      781 ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt
      841 catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg
      901 tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa atgattacga
      961 ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa
     1021 cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa aaaactgccg
     1081 aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga
     1141 tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt
     1201 gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg
     1261 cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga
     1321 cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt
     1381 ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa
     1441 tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca
     1501 acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac
     1561 ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat
     1621 atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac
     1681 gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa
     1741 ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc
     1801 aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat
     1861 ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt
     1921 cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag
     1981 ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa
     2041 tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa
     2101 aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc
     2161 gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc
     2221 attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt
     2281 gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta
     2341 tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg
     2401 ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
     2461 tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa
     2521 cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca
     2581 tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt
     2641 tcataattga accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga
     2701 agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt
     2761 caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt
     2821 ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat
     2881 cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga
     2941 catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta
     3001 agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag
     3061 taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt
     3121 tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca
     3181 tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt tttcttttta
     3241 caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc
     3301 gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag
     3361 tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt
     3421 atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc
     3481 gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa
     3541 agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg
     3601 caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca
     3661 atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct
     3721 tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa
     3781 ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg
     3841 agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt
     3901 ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc
     3961 ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt
     4021 caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc
     4081 accacccata tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa
     4141 caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa
     4201 caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca
     4261 acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct
     4321 tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg
     4381 gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc
     4441 atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta
     4501 ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac
     4561 acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga aacaccacta
     4621 caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct
     4681 aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa
     4741 caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat
     4801 gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa
     4861 tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa
     4921 cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg
     4981 tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt
     5041 cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt
     5101 acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc
     5161 tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc
     5221 taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag
     5281 ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg cttgctgctt
     5341 cacctagccc ctgtactcgt gtttgattgg cgacttgacc aaattgattc ccgacgtcta
     5401 ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat aaagcaataa
     5461 agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa taagaatctg
     5521 ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc gctaatatcc
     5581 ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc
     5641 aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct cttaatagac
     5701 gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa acagctgtac
     5761 tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga attccgactg
     5821 cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca ataccagata
     5881 ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc atgaccttcc
     5941 ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg
     6001 aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag
     6061 cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt ggacgatcca
     6121 ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat tgggtcaaca
     6181 tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact tttgttccaa
     6241 tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat aatagtaaaa
     6301 taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc catttcctat
     6361 cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt cgccacactt
     6421 attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg aagacaacta
     6481 accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta tatccgtgaa
     6541 acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca atcacgatcg
     6601 acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg accatagaag
     6661 atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt ttcatttgtc
     6721 ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca
     6781 taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg atcgcacctg
     6841 tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga atacctggaa
     6901 cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc ccatgcgaac
     6961 gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat tggatgtctg
     7021 tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt cccaacgaga
     7081 tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat ctatattcat
     7141 taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc
     7201 ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca agtttagctt
     7261 tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga gcgccatcaa
     7321 ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct ttgataactt
     7381 taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag
     7441 cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg ccaaattctt
     7501 cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt aactcagcaa
     7561 cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc ttttttattt
     7621 aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac tgctttgaca
     7681 gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga tagaagtcct
     7741 tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac tttaccttcg
     7801 atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc catgattttc
     7861 gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt gaatacttcg
     7921 tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt tttgataact
     7981 ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc atcaactgtt
     8041 aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc gcttacttca
     8101 tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt tcacctcctg
     8161 aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag ttactttgct
     8221 caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc gctttgctcg
     8281 aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc acttcgttca
     8341 tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta ttgaatatat
     8401 tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct tcggtacaag
     8461 ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga attatttttc
     8521 agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa catggatacc
     8581 aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag ctgctgatgg
     8641 tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt tttcatcgtc
     8701 aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac ggtaagttac
     8761 tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg taccagtttt
     8821 agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta cagtagccat
     8881 catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac
     8941 catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg ctttgtctcc
     9001 tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata caacagcacc
     9061 acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt
     9121 tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg caacaggata
     9181 aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct ttttagccat
     9241 tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc tatatctttc
     9301 aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct acagtgatcc
     9361 ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta
     9421 ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact ttcgcaactt
     9481 tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc aatagaacag
     9541 cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca gaaataacaa
     9601 ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt gtgaatccca
     9661 tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga gttgctttac
     9721 ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga catacctcct
     9781 taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt tatctgatct
     9841 gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat agcaaattta
     9901 tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa acgctaaatt
     9961 tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac acttgcgcta
    10021 gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt gtacaaagat
    10081 tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa gtgtctttga
    10141 tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag ctgctagaat
    10201 cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata tttatctata
    10261 tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag ttggcggcct
    10321 gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg catatgaagc
    10381 cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa
    10441 gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga tcggcggtgg
    10501 caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg gtactcctac
    10561 cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta gtatactttc
    10621 tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc
    10681 cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa gactcaaaca
    10741 gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa tgtttttatc
    10801 gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat tgatgattgc
    10861 agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca tggaaaaaaa
    10921 cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct ccgtcacagg
    10981 aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag actttttaag
    11041 tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa
    11101 aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct
    11161 gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat tcttatttac
    11221 cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg cagaaggggg
    11281 atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa
    11341 aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa atatgatggg
    11401 actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt
    11461 aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg aaagtgtcat
    11521 tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt
    11581 caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac ttgcggaaga
    11641 gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga gattagttat
    11701 cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt tattaatcca
    11761 tcaatttcgt atattttact tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg
    11821 acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca cctagattca
    11881 acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc agagctttga
    11941 agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt ttgttgacta
    12001 cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt tgacgtccac
    12061 catgattgga tacccagata ccatctgccc ctgcagagat agcgattact gcatcttctg
    12121 gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt gttttgatgt
    12181 catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct tttccttcgc
    12241 cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt aatggaaatt
    12301 ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct gccgttaaaa
    12361 tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg aatctgtcgt
    12421 ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt
    12481 tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca gctactcctt
    12541 tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga
    12601 taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct gagtcgattc
    12661 cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt tctttcatcg
    12721 tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt
    12781 ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct tcattacttg
    12841 tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa tcatactaaa
    12901 cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc tttccaaata
    12961 aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg agaggcattt
    13021 tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa cgcatagacc
    13081 gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc tagttgacca
    13141 gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg gtaatcttga
    13201 tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga ctgcgcacta
    13261 tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac ttgtggcaat
    13321 gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc catggaatgc
    13381 cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag acaagttttg
    13441 atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac ttggaccatc
    13501 ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc tgttcctact
    13561 gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa catcccttta
    13621 aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct
    13681 atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt atttatcttc
    13741 gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca
    13801 cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat atagaaaaaa
    13861 gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt aatcgatttt
    13921 atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat cgatgttgac
    13981 tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc
    14041 gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt gtcgcaggct
    14101 cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg ctggtttgct
    14161 tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga caacatacca
    14221 tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt tatgaacgat
    14281 ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa cgacacggaa
    14341 aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt ttactttgtt
    14401 ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt ccattgaagg
    14461 ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc
    14521 attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag ccaaataagc
    14581 tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa gtttcgatga
    14641 ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt ttgatttcat
    14701 cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt atttcgtttc
    14761 tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca aacgttcgcc
    14821 gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg aacaagcgag
    14881 tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga cttatacttc
    14941 ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc tattgttttg
    15001 gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt ggaggtatag
    15061 cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt acagagacta
    15121 gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt gtgccgatct
    15181 tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc tatcgtcata
    15241 aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac gctacaaagt
    15301 tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag cttccttata
    15361 ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt acgttgctta
    15421 atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca attgcttcca
    15481 caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac agcagcattg
    15541 ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc
    15601 taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta gagaaaaaga
    15661 attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa
    15721 aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac gcttttcaaa
    15781 atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga cgataaactc
    15841 acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg gaactaacat
    15901 ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt
    15961 tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca atgtttggcc
    16021 gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga cgatgacccc
    16081 gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca aactttctga
    16141 aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta acgctagata
    16201 aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc ttttcgtttt
    16261 ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg atgaaccgcg
    16321 gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc cgtcttatct
    16381 gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg
    16441 tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag taggatctgc
    16501 aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat ttgagcaacc
    16561 tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc cgaaccttgg
    16621 aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt gtttttcggt
    16681 cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt aattttgtaa
    16741 cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc ttgctaatta
    16801 ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag caggaggaaa
    16861 tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga aatggctata
    16921 aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat acacttcatt
    16981 atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa gaaacattga
    17041 ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg atcatcgtag
    17101 gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa gtagaattcc
    17161 cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca ggccttccaa
    17221 aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa aaagtccaca
    17281 taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat aatttcggta
    17341 taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa aagcgattga
    17401 acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct ttattcactc
    17461 aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt tcaaacgcat
    17521 ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt gttccaactg
    17581 ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat ttctttaaaa
    17641 tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa tatttccatc
    17701 actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt caagaagatg
    17761 gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa cagttgtttt
    17821 ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta ttcggaaaaa
    17881 aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa ttctttgccc
    17941 aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc attgaaaaca
    18001 ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca aatgtttgat
    18061 tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat gttggtgtga
    18121 atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac accccaccga
    18181 taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct gagtttttta
    18241 ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag aaagtattta
    18301 aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga accattagaa
    18361 gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt actttgcgat
    18421 tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct ttgcgattaa
    18481 atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca taagacaaga
    18541 aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc gcacttagct
    18601 caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca tcgattgttt
    18661 tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa
    18721 ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc tttcctactt
    18781 cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga ttaaaataaa
    18841 gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt tccttgtcat
    18901 tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc aaggcgattt
    18961 tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag aatgtggcaa
    19021 caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc aatgtccaaa
    19081 tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc taacgcaaaa
    19141 aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa atcagtgtag
    19201 aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg accaacaatc
    19261 ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc agcaaaaaag
    19321 ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt catttttggt
    19381 atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag agcggtaaca
    19441 atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg aaagacagct
    19501 aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt agcgattgaa
    19561 actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga ttgatttgct
    19621 tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc cattccaatc
    19681 agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac ttcttgaaaa
    19741 gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc cacttctgcc
    19801 actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt tttcatcaac
    19861 gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc aatgagataa
    19921 aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga tagttcctgt
    19981 acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt aaaattttcc
    20041 gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc gagattgcca
    20101 ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat tggaaaggcc
    20161 aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata tttataaaaa
    20221 tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa
    20281 gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct tttcttcttg
    20341 taagattgta agaatcggca acaatactgt atttagttca gtagttgtta cttggagctg
    20401 acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg ttttctggga
    20461 gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt agagattctt
    20521 tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg caaagaattt
    20581 ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga ttcagctcct
    20641 tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc ggctcatcta
    20701 aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc
    20761 caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact aattgaatca
    20821 cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg caaaaataat
    20881 cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga acgaccccaa
    20941 tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat ttaatggttc
    21001 ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc ccactgccgt
    21061 ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa tgattcaatg
    21121 ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc atgaaaaact
    21181 ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa ctagtgacga
    21241 ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca taaatctcgg
    21301 ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt aagtcaagga
    21361 ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg tgtcagttat
    21421 ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt atgtgccttt
    21481 ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc
    21541 caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg taatcatccc
    21601 cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt acgaaaaaaa
    21661 gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac tcattgggaa
    21721 aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga aacacaatta
    21781 gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga caatgtcggt
    21841 catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca
    21901 aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat ggatcggatt
    21961 cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc cttagagttt
    22021 ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga aggattgaat
    22081 gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa
    22141 catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt ttatcgcttg
    22201 caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat tggtttgaaa
    22261 acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca agaaaaccag
    22321 caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca gtagtcatta
    22381 ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat
    22441 ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg aaacccatcg
    22501 tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg atgctgccaa
    22561 agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat tcaaagatga
    22621 agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca aacagaactt
    22681 gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat ttggtaatga
    22741 aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata gtcatgctta
    22801 tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa
    22861 taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt acatcagcca
    22921 attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt tttatcagca
    22981 tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg catcattcag
    23041 gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat
    23101 caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg cggaaaaata
    23161 ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg ttctacatca
    23221 ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa gtcattgcca
    23281 gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa gtaacgcccc
    23341 ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc taaccaacga
    23401 tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat aaaatcgtag
    23461 ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat cacttctgct
    23521 gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc ttctccaaca
    23581 aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt ttcattcgtc
    23641 ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt tgtatagttc
    23701 ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag tttggtccgg
    23761 agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt catccggctt
    23821 gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa aatcaacagt
    23881 tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg ctgatattga
    23941 tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg gattgagcaa
    24001 caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc atttgcatca
    24061 aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat gatacccgtg
    24121 atttctccca tatctccatt gaatacattc aattcgggag tattgactaa ttggaggact
    24181 ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc aggtttccct
    24241 ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg tccccgatac
    24301 attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc tttttccaca
    24361 attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga acgatctttt
    24421 tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc aagaggaata
    24481 atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg aatttgttcg
    24541 atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg atctttgtct
    24601 cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt agccagccat
    24661 gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc tttcgtactg
    24721 actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt actggcaggc
    24781 agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc aagtagaata
    24841 ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa
    24901 agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc tgttaaaata
    24961 aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc atactgtatg
    25021 ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg ataatggatt
    25081 tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc agaaaagtac
    25141 aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc aacaagttgg
    25201 ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt ttctaaaata
    25261 cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca
    25321 tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc tgctgcaata
    25381 ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat gtcttcgact
    25441 agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc ctggtagata
    25501 ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac ttgatccatc
    25561 ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt caaaccggag
    25621 atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc tcccagcaat
    25681 tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct ggaaagatac
    25741 gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag tttaagctgc
    25801 cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc attttcttga
    25861 atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc tgtggtttca
    25921 ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca
    25981 actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct ccttcttcta
    26041 tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc atactttcag
    26101 ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac tactattgaa
    26161 caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt gaatggctgc
    26221 agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt gggcaaccgc
    26281 atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg
    26341 agaaggatca tacagatcta aatgattacg caaattcggc agattatccg gatatttctg
    26401 gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc ttagcttatc
    26461 tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg ctgtatcacg
    26521 tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta cctttttccc
    26581 aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat tgctcccttg
    26641 aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata gttccatacg
    26701 cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat ccagcttgtc
    26761 attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa gtgcttcata
    26821 cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat cattcatagg
    26881 gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac cggttgtttc
    26941 ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac tatatacttt
    27001 atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta aaaaagcttc
    27061 taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg cccgttttag
    27121 atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag tattcgtggc
    27181 tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa ataaaacgat
    27241 atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct gacaatttga
    27301 tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt
    27361 gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca
    27421 acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat taaaaactgc
    27481 tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta atcttcttca
    27541 aaggattctt atataagtct gctttgattc gctcttctac ctgccattct aaaggtgaca
    27601 tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt acacgctgac
    27661 gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg gaataactct
    27721 gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa ttttcttgtg
    27781 gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct tggtaatgag
    27841 aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc aaattggtca
    27901 aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag actccttctt
    27961 ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt tcattcaaga
    28021 agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa
    28081 tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt ggaacgaaca
    28141 ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg
    28201 aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt tttacttctt
    28261 cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg cgtcctcggt
    28321 caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta gatgctcact
    28381 ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata aaggagaaga
    28441 ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag caattgctag
    28501 aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac
    28561 tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg taatgacgat
    28621 tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa tcatgcatac
    28681 tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt gtcttactcc
    28741 ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag aagggttgca
    28801 gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg aagcaatcga
    28861 ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac
    28921 tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg gccgcttaat
    28981 cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta aagaattgaa
    29041 cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa
    29101 aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta cataaaatag
    29161 cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc gctcaaagtc
    29221 gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat tagcatcggg
    29281 attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat ccctatgaca
    29341 aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt gatccccgta
    29401 acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac aaatccatga
    29461 tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg ggttccagca
    29521 ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc ttcacttacc
    29581 aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc
    29641 agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc ttccacattg
    29701 accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc gatgatatca
    29761 tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag agctaagaag
    29821 tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg aactgaaagc
    29881 actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc
    29941 agagaaataa cccctactag ctgaccttct tgattaacga catagatgta atagattgtt
    30001 tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt ttgattggct
    30061 acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc gtaatggaga
    30121 agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt agcaatttgc
    30181 tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc agaaagcata
    30241 tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc gttgtcttct
    30301 tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata gatcagctgc
    30361 ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg catctctaaa
    30421 aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa ctgggcaaat
    30481 ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc
    30541 gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc aactcttctg
    30601 taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt attcccagat
    30661 ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa
    30721 ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg atttctgtta
    30781 gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag accattcgct
    30841 tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt gccgataaat
    30901 ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt tcacgaagtt
    30961 gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa ccagatgtat
    31021 cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt tttttataat
    31081 aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga atagacgaaa
    31141 cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata tcgatcggtg
    31201 tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg gttaccttat
    31261 catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg atttgaccgc
    31321 cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt aaaaaatatt
    31381 ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca atcctcacta
    31441 ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc aaaatggatc
    31501 cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac attcaattga
    31561 tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca ttcatcatgg
    31621 gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga agcaatctct
    31681 gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact tttattatat
    31741 gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact ttcaaacagc
    31801 tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat cgttgattca
    31861 ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg aacatccagc
    31921 aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag ttcttccagc
    31981 tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc
    32041 acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg
    32101 actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt
    32161 gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac gatcgctact
    32221 ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat agtagacatc
    32281 atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt cttcacggat
    32341 actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa gtcgttcact
    32401 catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa tcgttgccca
    32461 gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct caccgctgat
    32521 cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg cttttttatt
    32581 tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga tgatctcaac
    32641 aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa tatcagacat
    32701 ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta caatactatc
    32761 gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt gttcacggaa
    32821 ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt catatggtgc
    32881 taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa cattcattgt
    32941 ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg accaaaaaaa
    33001 gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga
    33061 atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact atcaattaac
    33121 agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg aacagctgaa
    33181 gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat atacattaaa
    33241 aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag aagaagcaga
    33301 agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa aactttctga
    33361 tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa aacgggctga
    33421 attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca atctacacga
    33481 ttttgaactg gaactagagg taaggggatg catctgtggg aaaaattgct tttataaatt
    33541 ttttaaaacg tttttcttta ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg
    33601 aagctaaaaa ttagaaacct ttcattttca caaggcactt gagttcattt ttttactttt
    33661 ttctgttaag ttataggatg tgaaagaatc tagtcactag ttgtatagaa tctattttgg
    33721 gggagattca tatgatcgaa atctatttat ttgttaatcc tttaggggaa tactgtttcg
    33781 aaatggaaca acagttatta caatttatcg aagaagagta cgggacacca tcaaaagaaa
    33841 agatgcaatt tcgctttctt cctttggtca atttgcagac aattggggat gtcatgcaac
    33901 aaaaaggaat ttctcagaac aatcttgagg aacgtaatca cttattctca atgacttatt
    33961 ctgcagcatt ggactgcaaa gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt
    34021 tgatcaaatt gcaagaagca gtcggctgta atgacatacc atattctcaa gaacttgctg
    34081 aatccatatt tgaagaaatc ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg
    34141 acttcgttaa agaagtattc ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa
    34201 aacacccttc tgcagtcgtt tacaattatg cctgcgaccg tgattttggt gttttagtag
    34261 aagtaaaagg tacaagtagc ctttggagaa tcacagagct gtgtaaaaca acagataact
    34321 catatcaagt ttttaatggt gaacatttac atacaaatga ttctcagttc cattcaagaa
    34381 ctagccattt acacttgctt tctaattaaa aaaatacgtc gactccctta caagagagcc
    34441 gacgtatttt tttcgtttct attcgagaaa atcaaaaata gaaagcctta taaacgatcg
    34501 atcaattctt ctaattcatt tagacgttgt tcaaacatcg acattgcatc ttcaatatat
    34561 gcagcctgtg tcatatcaac tcctgctttt ttcattactt ccacaggata atcgctgtta
    34621 cctgctttta ggtaagccaa gtagttctct agtgcctctg gttcctggtt tagtattttc
    34681 ttcgccagtg ctgaagcagc agaaaagcca gtcgagtatt ggaaaacata gtaattataa
    34741 taaaaatgcg gtatccgtga ccattcgaat ttaatttctg ggtcttcttc gactgctgga
    34801 ccataatatt ttgcattcaa tttaccataa ctatcactta ggtattcact agtcaatggc
    34861 acaccttttt catcttccgt atgcatgaaa tgctcgaatt cagcgaattg tgtctggcgg
    34921 aaaactgttc ctttaaaccc atccaagtaa tggttgagta cataagcccg tacacgaggg
    34981 tctttttctg tttccaataa atactccgtt aggatatttt cattcgttgt cgaagcaatt
    35041 tctgctaaaa agatggagta gtcgccgtac acataaggct ggttcgaacg agtgaaataa
    35101 ctatgaacac tatgtcccat ttcgtggaca agcgtaaata gctgatccag cgtatcatgc
    35161 caattcaata aaatatatgg atttgtgtca tagcttcccg aagaataagc accgcttcgt
    35221 ttccctttat tttcgacaac atcgatccaa cgttcagaga atgctttttc tacgatggcc
    35281 atgtattctt cacccattgg tttcagtgct tctaaagctt tttcttttgc ttcttcgtac
    35341 gtaaaggtaa ttggagcttc ccctaagacc ggtgtataaa gatcatacat gtgcagtttt
    35401 tccacttcta ataaacgttt ccgtaattcc atgtatcgat gtaacaaagg caaatgcttg
    35461 tttaccacgt ctaccaaagt atcgtataca ctttcaggaa tatgattatt gctcaaagac
    35521 gcttctctgg cagagctgta attacggact ttcgctttaa aattatgtcc ttttatatgt
    35581 gtgcctaaag tagaagcaaa tgtatttcta aattgttcgt aaacactgta caatccctta
    35641 aatgctgctt cgcgcaccct tcgatccgtg ctttctagca actgaccata cacgccatga
    35701 gataattgga ctatttcacc attttctcct tcaatcgttg gaaaaactag atctgcatta
    35761 ttcaaaacag caaatgtatc acttgaagca tcaaagattt cacctgctcc agcaagaaga
    35821 gattcctgct cagcagataa gacatgggct cgattatcta ctatttgctg gatataatgg
    35881 cgatagactt ccaattttgg ttcttctttg aaatattgcc aaatctggtc atctgacaat
    35941 tgcaatattt ctggttcaaa ccaggaaacg gcttcactaa ctttcgaaaa cagactgctt
    36001 gctcttgcat aaagcgcttg gtaatctgta tttccagtgt cttgatcgtt tttaaggtgc
    36061 gcatatacat aaatgacttc agtttggcga taaaccctca atacgaattc aatcgcattt
    36121 aaaaattgag aagcgccttg atcaagtgtt cctttgtgtt tttcagattg ttttaactct
    36181 tctgataatt ccaaatattt ctcatcaaac tcttggtcgc ttgaaaagat cttggtcaag
    36241 tcccaagtta aattttcagg caattcttct cgttttggca actgctttac ttccatgaaa
    36301 tccactcctt ttcttccctt agctctatct atcaaaatag atcatagtct aacatccaca
    36361 tgtcacagca tctaactttt ctactcattc atgtgtctgc ttccatcaat tttagcataa
    36421 ctaaatcttg ttttacatag aaaaccgatt cctagcctat ttttatactt actatttttt
    36481 caaagaagac aagtgtatgc attcgttaaa aaactgctga tagaccatct cttcatcaat
    36541 cataggaaaa agccattccc ttttatgttc ttttatttgg caaagccatg agttgaaatc
    36601 aggaactttc tggttttcca aggcttcttc gtacaacatt cgataaagaa aaacctgttc
    36661 ttgtaaataa aagaaaaacc gacttttttg atagatccac gatgatagat gcaaaagatg
    36721 tttatgacgc aagtagcatt gttcttgtat ggatactgta ttcttttgct tgtgaataag
    36781 ttggcgtgtc agccatttct tcccatcttc actcactttt tctatttttt tcatttggtt
    36841 cattttaatt tcgttactag tgaatatttc tttaaggtcc ctactagaaa aagtccagca
    36901 gctactgcaa aaggaaatat gccctgatac agtctctgta atatgatgat acaaataaag
    36961 cttttgtttc ttgagatcag ctttccagca atgcactcct ctttttctat tatagtagca
    37021 gcaacttttt tctatcaatg agaattgcga ttgtccaaga aaagaattac caagtatcca
    37081 ccatacttgg taactgttat tcaaataatt ttgggttctt tcaataaatc tctggtgaga
    37141 cagtctagaa cattggattt ctattgctag attcgccaac aaaatatccg gcctttgccg
    37201 caacctcggc aaataggctt ccaaaaatac tggttctact gactgtccca gccaatccat
    37261 gaagcattcc tttaactgta agtgctcttt tgattctctt tcactaaaga ccgaacagtt
    37321 atctgatttt tgatgggcaa aatgagcaca ttttttcttt ccctttttca aaatcactct
    37381 ttttcgacag ccacgacaat aatatttctt tcctttttct gcatgtttag caaaacagca
    37441 cttattatat tcattcattg caagcagcat ttttctccct ctgaacttat ttacgtctaa
    37501 gaatttattt acacgaaaaa agagggcgtg aaaaagctat tctgcatcaa gtaataagaa
    37561 cagctaaaca aaaatagttc ccaatatttt tcgtttggtt gggcttgtta ccgcagatgc
    37621 aagcttttga acacatttat acgaaaaaaa gactgcgaca caatttttgt cacagtctct
    37681 tactataatg ctgatttata caaaataaaa tttcgttcgc tcgatagcat tacgcgccat
    37741 cagacattgt ccatattcat tcaatgtttc tggtgtacga tcgctctttt cagcaaactc
    37801 caaaattcga gcaatctgat tctctacatc cgcttggttg agattttcca tccagaagtg
    37861 aacgactagg taatagcgct cttcgaataa atacaaatca gtccaagcag actctagttc
    37921 gacatctgtt gcaagttgga tcacttgttc aaaatcttca aaagcaaaag ttgcttgtct
    37981 ttcctgttct tctagggcgc ttccagataa ttcttcctcc ggatcgctgc caataatctg
    38041 ctgacgaaga aagtcactca catcttctga atcaacagta gaatcttctg attgagaaat
    38101 ttcttcatcc ggaagatttt tactgataaa cagttctaat ccgtctcctt ttggcaaaac
    38161 ttggaaagtg acagcttcgc ttcctcggaa ctcctcttcg atatcaactt cttccaaaat
    38221 actataaaag aaattttcga tttctttgtg attgccaagt aaatccaaaa atgaaacgcc
    38281 ccgttcctct aaatcttcat gaccaattag tacacggatc gtattttcat taatatgttc
    38341 catttccatt ttgctacacc tcactttgcc acaatgttta atatagattc attgtagcga
    38401 aatcagctta aatgtaaaga aaaaaaatct cgctgttaag cgaatttaat aaaaccagcg
    38461 gatttttcaa cctttcaagc tatcttattt aactataaca tttttggcag acgaatacta
    38521 tatagccgac ttttcagcta attttccctc tctatttttc gtgcaatcat catcttctac
    38581 taccttttta tacatctaat caagaatact ttttgtaaaa acaaaaaaac ttggaaaaac
    38641 tatctttgt
//
LOCUS       DAZKOK010000013        35388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000013 DAZKOK010000000
VERSION     DAZKOK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.013"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..288
                     /locus_tag="ACG90W_000639"
     CDS             <1..288
                     /locus_tag="ACG90W_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477027.1"
                     /translation="LKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            347..787
                     /locus_tag="ACG90W_000640"
     CDS             347..787
                     /locus_tag="ACG90W_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477028.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            799..1467
                     /locus_tag="ACG90W_000641"
     CDS             799..1467
                     /locus_tag="ACG90W_000641"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0477029.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1531..2154
                     /locus_tag="ACG90W_000642"
     CDS             1531..2154
                     /locus_tag="ACG90W_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0477030.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2210..2596
                     /locus_tag="ACG90W_000643"
     CDS             2210..2596
                     /locus_tag="ACG90W_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0477031.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2809..4200
                     /locus_tag="ACG90W_000644"
     CDS             2809..4200
                     /locus_tag="ACG90W_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0477032.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4811..5179
                     /locus_tag="ACG90W_000645"
     CDS             4811..5179
                     /locus_tag="ACG90W_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0477033.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5359..6300
                     /locus_tag="ACG90W_000646"
     CDS             5359..6300
                     /locus_tag="ACG90W_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0477034.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6369..7100
                     /locus_tag="ACG90W_000647"
     CDS             6369..7100
                     /locus_tag="ACG90W_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0477035.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7244..9424
                     /locus_tag="ACG90W_000648"
     CDS             7244..9424
                     /locus_tag="ACG90W_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0477036.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9444..9926
                     /locus_tag="ACG90W_000649"
     CDS             9444..9926
                     /locus_tag="ACG90W_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0477037.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            9942..10703
                     /locus_tag="ACG90W_000650"
     CDS             9942..10703
                     /locus_tag="ACG90W_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0477038.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            10834..12168
                     /gene="murC"
                     /locus_tag="ACG90W_000651"
     CDS             10834..12168
                     /gene="murC"
                     /locus_tag="ACG90W_000651"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0477039.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12475..12960
                     /locus_tag="ACG90W_000652"
     CDS             12475..12960
                     /locus_tag="ACG90W_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0477040.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            12953..13636
                     /locus_tag="ACG90W_000653"
     CDS             12953..13636
                     /locus_tag="ACG90W_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0477041.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            13762..16407
                     /gene="polA"
                     /locus_tag="ACG90W_000654"
     CDS             13762..16407
                     /gene="polA"
                     /locus_tag="ACG90W_000654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0477042.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16452..17288
                     /gene="mutM"
                     /locus_tag="ACG90W_000655"
     CDS             16452..17288
                     /gene="mutM"
                     /locus_tag="ACG90W_000655"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0477043.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17252..17881
                     /gene="coaE"
                     /locus_tag="ACG90W_000656"
     CDS             17252..17881
                     /gene="coaE"
                     /locus_tag="ACG90W_000656"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0477044.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            17933..18265
                     /locus_tag="ACG90W_000657"
     CDS             17933..18265
                     /locus_tag="ACG90W_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0477045.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG90W_000658"
     CDS             18577..19068
                     /gene="nrdR"
                     /locus_tag="ACG90W_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0477046.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19091..20464
                     /locus_tag="ACG90W_000659"
     CDS             19091..20464
                     /locus_tag="ACG90W_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0477047.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG90W_000660"
     CDS             20464..21390
                     /gene="dnaI"
                     /locus_tag="ACG90W_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0477048.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21519..21959
                     /locus_tag="ACG90W_000661"
     CDS             21519..21959
                     /locus_tag="ACG90W_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0477049.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22103..22804
                     /locus_tag="ACG90W_000662"
     CDS             22103..22804
                     /locus_tag="ACG90W_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0477050.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            22801..23922
                     /locus_tag="ACG90W_000663"
     CDS             22801..23922
                     /locus_tag="ACG90W_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0477051.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            23937..25169
                     /gene="pepT"
                     /locus_tag="ACG90W_000664"
     CDS             23937..25169
                     /gene="pepT"
                     /locus_tag="ACG90W_000664"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0477052.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25301..26209
                     /locus_tag="ACG90W_000665"
     CDS             25301..26209
                     /locus_tag="ACG90W_000665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0477053.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26244..26516
                     /locus_tag="ACG90W_000666"
     CDS             26244..26516
                     /locus_tag="ACG90W_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0477054.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26527..27573
                     /locus_tag="ACG90W_000667"
     CDS             26527..27573
                     /locus_tag="ACG90W_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0477055.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            27824..30433
                     /gene="clpB"
                     /locus_tag="ACG90W_000668"
     CDS             27824..30433
                     /gene="clpB"
                     /locus_tag="ACG90W_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0477056.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30584..31270)
                     /locus_tag="ACG90W_000669"
     CDS             complement(30584..31270)
                     /locus_tag="ACG90W_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477057.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31248..31742)
                     /locus_tag="ACG90W_000670"
     CDS             complement(31248..31742)
                     /locus_tag="ACG90W_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0477058.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(31762..32982)
                     /locus_tag="ACG90W_000671"
     CDS             complement(31762..32982)
                     /locus_tag="ACG90W_000671"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477059.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33129..34964
                     /gene="lepA"
                     /locus_tag="ACG90W_000672"
     CDS             33129..34964
                     /gene="lepA"
                     /locus_tag="ACG90W_000672"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0477060.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35160..>35388
                     /locus_tag="ACG90W_000673"
     CDS             35160..>35388
                     /locus_tag="ACG90W_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477061.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGK"
BASE COUNT        11877 a         6229 c         7592 g         9690 t
ORIGIN      
        1 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
       61 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      121 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      181 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      241 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      301 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      361 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      421 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      481 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      541 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      601 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
      661 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
      721 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
      781 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
      841 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
      901 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
      961 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1021 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1081 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1141 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1201 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1261 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1321 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1381 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1441 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1501 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1561 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1621 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     1681 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     1741 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     1801 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     1861 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     1921 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     1981 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2041 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2101 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2161 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2221 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2281 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2341 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2401 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2461 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2521 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2581 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     2641 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     2701 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     2761 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     2821 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     2881 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     2941 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3001 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3061 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3121 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3181 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3241 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3301 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3361 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3421 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3481 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3541 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3601 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     3661 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     3721 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     3781 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     3841 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     3901 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     3961 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4021 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4081 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4141 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4201 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4261 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4321 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4381 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4441 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4501 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4561 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4621 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     4681 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     4741 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gatcgtttag
     4801 gaggaaacag atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa
     4861 aaatgcaacg ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg
     4921 gacgcttata attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg
     4981 agcatttgtt gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta
     5041 cagtaaagat atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga
     5101 tatggtacag cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt
     5161 tgcggttaca ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc
     5221 gaagtctttt taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta
     5281 tacacaagca aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa
     5341 gaaggaagga agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc
     5401 gaagaaccag aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa
     5461 ggtcgtattc tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac
     5521 acgactgcct atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag
     5581 atcgatccaa gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt
     5641 gtaagtttaa agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca
     5701 ccgcaagaat ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat
     5761 gattatgaat atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt
     5821 cgagagcttc cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg
     5881 acctactgta ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga
     5941 ttcaaagatg tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc
     6001 caaggagaat tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc
     6061 aaccaagtag ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga
     6121 tatatcaatt gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa
     6181 gaaaaacatt taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt
     6241 caagaccatg gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag
     6301 aaaacaatag ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg
     6361 tgatccacat gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt
     6421 ttgaaaaact tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc
     6481 tcaatgatcg aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag
     6541 aaacaggagc catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat
     6601 caccggagga ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc
     6661 tgattcaccc aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat
     6721 cgagatattc agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa
     6781 attatctttt tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc
     6841 taagagactt tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga
     6901 caattgtatt tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc
     6961 tctggattca ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc
     7021 atggggttgt ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag
     7081 gaccttggca tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct
     7141 ctttttattt ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat
     7201 gatagaataa agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa
     7261 ccagacgatc attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac
     7321 tactgtactt aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa
     7381 agaaatgact ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta
     7441 tcttgaaaac ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa
     7501 actgactccc gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga
     7561 tttgtatcgt ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg
     7621 acttgaacca ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc
     7681 agaaaaatat attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc
     7741 tcatgagatt attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc
     7801 ttatacgtat aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa
     7861 aggcgtatat gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg
     7921 aatattagct actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga
     7981 tgaagcagca attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc
     8041 ttcttcattc gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat
     8101 cgagcgagaa ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt
     8161 cggcgaaaac ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg
     8221 atttgatccg gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt
     8281 tttagcaatc gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc
     8341 tggaccagct tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa
     8401 tggtacagcc agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca
     8461 taaaacctat tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat
     8521 tgcacgaaaa gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg
     8581 tcgcctgcaa gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg
     8641 ccagtaccaa catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga
     8701 aacagcggtt aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca
     8761 tatatcagga ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg
     8821 agaattcacc gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga
     8881 acaagcaatc ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat
     8941 ccatcccgaa agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa
     9001 agagcttggt acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc
     9061 tgaaagccta tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc
     9121 tggacgagat atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat
     9181 ggaagacttg aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg
     9241 cgcatttgtt gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa
     9301 aaaattcgtc aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat
     9361 agaacaagtc gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga
     9421 gtaacaatga ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga
     9481 tatcattaaa aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac
     9541 gtacaactgg cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat
     9601 ttgaagagtc agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc
     9661 atcttcatct agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga
     9721 aaaaaacagg agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt
     9781 attatcgatg ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca
     9841 aatatcgttg tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact
     9901 ttaatgaagc acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga
     9961 ggaatctaaa agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt
    10021 ttgaggaact gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg
    10081 ataaagcagc acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg
    10141 tggggcatgc gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag
    10201 atggcatgag tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg
    10261 ataaggcttt tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac
    10321 tggagattct tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga
    10381 cactgcaacg caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt
    10441 tcgcgaatgc tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta
    10501 cagcaaaaga actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag
    10561 aaatggaacg tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc
    10621 ctcacattga tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa
    10681 aaacggattt ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt
    10741 tggttgaaat tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa
    10801 agaatggacg ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat
    10861 tttgttggta tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt
    10921 ctaaatgtcc aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa
    10981 gcaaatatca cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt
    11041 gcaggaaatg catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg
    11101 gaagtgattc gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct
    11161 gtaacaggct ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt
    11221 gtccgtccaa caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa
    11281 ttcttttcat ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat
    11341 gcaatcatga ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta
    11401 tttacagctt tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac
    11461 gatgcttatt tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa
    11521 aacgatgata tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc
    11581 tatcatgggg atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc
    11641 ttaaatgctt taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt
    11701 gcagaagaaa tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca
    11761 gacatgaccg tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat
    11821 ggcgcacgcc aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt
    11881 acgcgtacga ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt
    11941 tacttatgcg atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac
    12001 cttggagcga aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg
    12061 ttagattatc atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa
    12121 caagcgtatg aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc
    12181 agtgtcctta taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt
    12241 tgcaacttat gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat
    12301 caaaaacatg agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt
    12361 ttcacgacct ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta
    12421 cgctactttt ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac
    12481 ataggtaagc cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta
    12541 tccttaacag aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat
    12601 gcaaatcctt tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc
    12661 gttccatcag tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga
    12721 ccaggatcac atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa
    12781 acactgactt ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt
    12841 tctgtagaag cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa
    12901 ccgccacaag ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa
    12961 tccaagtaca tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg
    13021 tatgggactt gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca
    13081 attagcaact tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact
    13141 gattttagta atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg
    13201 atttattgcc tactctcttt tgaatggcat aacattagcg gttactcttg catactacga
    13261 tattggaaca gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt
    13321 tgtcggagta acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt
    13381 aatcggagtg atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga
    13441 gctgttcatc tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca
    13501 aagcatccgt aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt
    13561 catggcattg aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt
    13621 tggacggaac aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg
    13681 tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact
    13741 ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt
    13801 gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac
    13861 ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa
    13921 gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa
    13981 atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg
    14041 ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat
    14101 gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc
    14161 gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat
    14221 atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa
    14281 tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct
    14341 gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa
    14401 tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa
    14461 gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat
    14521 acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat
    14581 aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc
    14641 gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag
    14701 gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca
    14761 gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta
    14821 tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa
    14881 gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga
    14941 atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat
    15001 atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac
    15061 ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg
    15121 cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa
    15181 aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg
    15241 gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct
    15301 gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg
    15361 aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa
    15421 aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca
    15481 ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc
    15541 gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca
    15601 ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga
    15661 ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt
    15721 tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa
    15781 catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta
    15841 tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg
    15901 aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc
    15961 cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa
    16021 tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac
    16081 cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa
    16141 cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg
    16201 atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt
    16261 catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg
    16321 aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg
    16381 ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt
    16441 gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt
    16501 tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac
    16561 cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta
    16621 gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg
    16681 aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt
    16741 tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga
    16801 caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc
    16861 ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag
    16921 caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag
    16981 atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca
    17041 aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg
    17101 gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag
    17161 cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga
    17221 aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg
    17281 caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc
    17341 caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac
    17401 tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt
    17461 gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga
    17521 aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca
    17581 aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga
    17641 aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca
    17701 gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag
    17761 tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg
    17821 aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg
    17881 agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc
    17941 attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct
    18001 caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga
    18061 aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa
    18121 gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct
    18181 cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta
    18241 tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat
    18301 gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta
    18361 agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg
    18421 ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg
    18481 acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat
    18541 cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat
    18601 aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt
    18661 cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga agctgctccg
    18721 ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga
    18781 ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc
    18841 aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt
    18901 ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca
    18961 agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg
    19021 gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg
    19081 aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag
    19141 aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact
    19201 aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc
    19261 tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc
    19321 gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa
    19381 agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa
    19441 agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg
    19501 attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa
    19561 aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca
    19621 agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga
    19681 agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc
    19741 aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga
    19801 tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc
    19861 tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa
    19921 agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa
    19981 cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc
    20041 aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct
    20101 tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt
    20161 gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc
    20221 acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca
    20281 agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg
    20341 acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga
    20401 gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat
    20461 ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa
    20521 aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc
    20581 gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa
    20641 ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat
    20701 gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca
    20761 ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca
    20821 aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg
    20881 agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa
    20941 ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg
    21001 agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt
    21061 gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt
    21121 tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac
    21181 gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca
    21241 tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa
    21301 gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa
    21361 atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc
    21421 tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc
    21481 gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta
    21541 tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag
    21601 gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga
    21661 gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa
    21721 cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt
    21781 cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa
    21841 atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga
    21901 tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac
    21961 caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt
    22021 taaggagata tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata
    22081 attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt
    22141 gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt
    22201 acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca
    22261 aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac
    22321 cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac
    22381 gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca
    22441 acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg
    22501 atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa
    22561 aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat
    22621 ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg
    22681 gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca
    22741 agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc
    22801 atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta
    22861 gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga
    22921 gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc
    22981 gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat
    23041 cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca
    23101 cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt
    23161 attgaagtgg agagttattt agtaaagact catgagattc attataaaaa actggccgtt
    23221 tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt
    23281 acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg
    23341 gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca
    23401 aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca
    23461 gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg
    23521 tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa
    23581 caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa
    23641 tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca
    23701 atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat
    23761 atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag
    23821 caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt
    23881 ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt
    23941 acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa
    24001 caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag
    24061 aattagaaga gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg
    24121 gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg
    24181 atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg
    24241 agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa
    24301 atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag
    24361 cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc
    24421 cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg
    24481 gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg
    24541 gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc
    24601 aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag
    24661 caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg
    24721 aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata
    24781 tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc
    24841 aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat
    24901 attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag
    24961 caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt
    25021 caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc
    25081 acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa
    25141 aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt
    25201 tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt
    25261 gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt
    25321 ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta
    25381 tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc catcgattga
    25441 tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg
    25501 tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca
    25561 tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct
    25621 caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg
    25681 tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc
    25741 agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt
    25801 ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca
    25861 ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac
    25921 aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc
    25981 cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac
    26041 gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc
    26101 tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt
    26161 atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt
    26221 aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc
    26281 ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa
    26341 ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt
    26401 tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta
    26461 taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag
    26521 caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt
    26581 gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa
    26641 ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa
    26701 atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg
    26761 aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca
    26821 ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga
    26881 gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta
    26941 gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt
    27001 atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc
    27061 agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt
    27121 ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa
    27181 accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt
    27241 gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt
    27301 ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct
    27361 gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca
    27421 gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc
    27481 tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa
    27541 gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat
    27601 caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat
    27661 atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa aagtcaaaaa
    27721 actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca
    27781 aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat
    27841 gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca
    27901 tcaagatatc gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg
    27961 aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct
    28021 actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca
    28081 gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt
    28141 tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt
    28201 ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag
    28261 aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata
    28321 cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga
    28381 tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct
    28441 gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt
    28501 acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc
    28561 attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa
    28621 agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt
    28681 aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc
    28741 tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga
    28801 aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga
    28861 agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa
    28921 catccatgac aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg
    28981 gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga
    29041 aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat
    29101 cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt
    29161 gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac
    29221 agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca
    29281 cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg
    29341 tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga
    29401 gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt
    29461 aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa
    29521 tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc
    29581 ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt
    29641 cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt
    29701 tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt
    29761 gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac
    29821 ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca
    29881 tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa
    29941 agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca
    30001 gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt
    30061 caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat
    30121 tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca
    30181 attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac
    30241 gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat
    30301 cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa
    30361 atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga
    30421 agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt
    30481 gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc
    30541 cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt
    30601 tttctaattt ataactttca aagtaacgct catctttttt actttgagac catgcaaaca
    30661 atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg
    30721 ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta
    30781 agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg
    30841 caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca
    30901 agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga
    30961 ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga
    31021 tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt
    31081 tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg
    31141 tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa
    31201 cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa
    31261 gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact
    31321 tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca
    31381 gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca
    31441 gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc
    31501 ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt
    31561 tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct
    31621 tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact
    31681 aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc
    31741 atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat
    31801 gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa
    31861 ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac
    31921 ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc
    31981 gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc
    32041 tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa
    32101 atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt
    32161 tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca
    32221 taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc
    32281 agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc
    32341 tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa
    32401 taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc
    32461 aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct
    32521 gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat
    32581 actgtaagga tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac
    32641 gataattaga tcttgcatca atccatggtt agcatattct ggataagttc caactccagt
    32701 cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat
    32761 ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg
    32821 ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc
    32881 agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac
    32941 cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat
    33001 tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa
    33061 aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag
    33121 gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga
    33181 tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc
    33241 acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg
    33301 aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag
    33361 aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct
    33421 ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg
    33481 aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag
    33541 tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag
    33601 acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga
    33661 ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg
    33721 caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa
    33781 atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg
    33841 gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact
    33901 ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac
    33961 gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc
    34021 gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg
    34081 atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag
    34141 aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg
    34201 atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat
    34261 ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag
    34321 attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca
    34381 tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat
    34441 tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt
    34501 ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat
    34561 tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga
    34621 atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg
    34681 gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa
    34741 tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa
    34801 acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac
    34861 aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct
    34921 tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa
    34981 aaacctatta aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga
    35041 ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt
    35101 cttgaagatt tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca
    35161 tggttgataa ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc
    35221 aaaaaaagtt agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag
    35281 ggtcaagaaa aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa
    35341 ctctaataaa agatgaggaa gataagattg agattctcat cggtaaac
//
LOCUS       DAZKOK010000014        34465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000014 DAZKOK010000000
VERSION     DAZKOK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.014"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..1462)
                     /locus_tag="ACG90W_000674"
     CDS             complement(176..1462)
                     /locus_tag="ACG90W_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0477062.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="ACG90W_000675"
     CDS             complement(1455..2723)
                     /locus_tag="ACG90W_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0477063.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="ACG90W_000676"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="ACG90W_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0477064.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="ACG90W_000677"
     CDS             complement(3495..3941)
                     /locus_tag="ACG90W_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477065.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="ACG90W_000678"
     CDS             complement(4034..4279)
                     /locus_tag="ACG90W_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0477066.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="ACG90W_000679"
     CDS             complement(4394..4972)
                     /locus_tag="ACG90W_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0477067.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="ACG90W_000680"
     CDS             complement(5049..5579)
                     /locus_tag="ACG90W_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0477068.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="ACG90W_000681"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="ACG90W_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0477069.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="ACG90W_000682"
     CDS             complement(6436..6831)
                     /locus_tag="ACG90W_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0477070.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /gene="cysS"
                     /locus_tag="ACG90W_000683"
     CDS             complement(6828..8240)
                     /gene="cysS"
                     /locus_tag="ACG90W_000683"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0477071.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="epsC"
                     /locus_tag="ACG90W_000684"
     CDS             complement(8237..8773)
                     /gene="epsC"
                     /locus_tag="ACG90W_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0477072.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8859..9098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9095..10555)
                     /gene="gltX"
                     /locus_tag="ACG90W_000685"
     CDS             complement(9095..10555)
                     /gene="gltX"
                     /locus_tag="ACG90W_000685"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0477073.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="ACG90W_000686"
     CDS             complement(10725..11882)
                     /locus_tag="ACG90W_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0477074.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="ACG90W_000687"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="ACG90W_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0477075.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="ACG90W_000688"
     CDS             complement(13349..13876)
                     /locus_tag="ACG90W_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0477076.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="ACG90W_000689"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="ACG90W_000689"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0477077.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="ACG90W_000690"
     CDS             14909..16156
                     /locus_tag="ACG90W_000690"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0477078.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="ACG90W_000691"
     CDS             16232..16576
                     /locus_tag="ACG90W_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477079.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="ACG90W_000692"
     CDS             complement(16693..17019)
                     /locus_tag="ACG90W_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477080.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17634..18836)
                     /locus_tag="ACG90W_000693"
     CDS             complement(17634..18836)
                     /locus_tag="ACG90W_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0477081.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18953..19468
                     /locus_tag="ACG90W_000694"
     CDS             18953..19468
                     /locus_tag="ACG90W_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0477082.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19667..20113)
                     /locus_tag="ACG90W_000695"
     CDS             complement(19667..20113)
                     /locus_tag="ACG90W_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0477083.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="ACG90W_000696"
     CDS             complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="ACG90W_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0477084.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21992..22621)
                     /locus_tag="ACG90W_000697"
     CDS             complement(21992..22621)
                     /locus_tag="ACG90W_000697"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0477085.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22605..23069)
                     /locus_tag="ACG90W_000698"
     CDS             complement(22605..23069)
                     /locus_tag="ACG90W_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0477086.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23556..24824)
                     /locus_tag="ACG90W_000699"
     CDS             complement(23556..24824)
                     /locus_tag="ACG90W_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0477087.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25055..25792)
                     /locus_tag="ACG90W_000700"
     CDS             complement(25055..25792)
                     /locus_tag="ACG90W_000700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0477088.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25859..26428)
                     /locus_tag="ACG90W_000701"
     CDS             complement(25859..26428)
                     /locus_tag="ACG90W_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0477089.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26556..27428
                     /locus_tag="ACG90W_000702"
     CDS             26556..27428
                     /locus_tag="ACG90W_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0477090.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27770..28642
                     /locus_tag="ACG90W_000703"
     CDS             27770..28642
                     /locus_tag="ACG90W_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0477091.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28639..30597
                     /gene="nagE"
                     /locus_tag="ACG90W_000704"
     CDS             28639..30597
                     /gene="nagE"
                     /locus_tag="ACG90W_000704"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0477092.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30929..31780)
                     /locus_tag="ACG90W_000705"
     CDS             complement(30929..31780)
                     /locus_tag="ACG90W_000705"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0477093.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31903..32370
                     /locus_tag="ACG90W_000706"
     CDS             31903..32370
                     /locus_tag="ACG90W_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0477094.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32633..33115)
                     /locus_tag="ACG90W_000707"
     CDS             complement(32633..33115)
                     /locus_tag="ACG90W_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0477095.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(33215..34078)
                     /locus_tag="ACG90W_000708"
     CDS             complement(33215..34078)
                     /locus_tag="ACG90W_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0477096.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9839 a         7260 c         6212 g        11154 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    17281 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    17341 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    17401 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    17461 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    17521 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    17581 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    17641 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    17701 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    17761 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    17821 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    17881 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    17941 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    18001 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    18061 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    18121 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    18181 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    18241 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    18301 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    18361 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    18421 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    18481 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    18541 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    18601 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    18661 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    18721 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    18781 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    18841 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    18901 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    18961 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    19021 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    19081 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    19141 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    19201 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    19261 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    19321 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    19381 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    19441 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    19501 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    19561 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    19621 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    19681 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    19741 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    19801 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    19861 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    19921 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    19981 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    20041 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    20101 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    20161 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    20221 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    20281 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    20341 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    20401 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    20461 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    20521 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    20581 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    20641 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    20701 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    20761 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    20821 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    20881 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    20941 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    21001 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    21061 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    21121 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    21181 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    21241 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    21301 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    21361 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    21421 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    21481 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    21541 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    21601 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    21661 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    21721 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    21781 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    21841 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    21901 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    21961 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    22021 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    22081 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    22141 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    22201 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    22261 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    22321 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    22381 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    22441 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    22501 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    22561 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    22621 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    22681 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    22741 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    22801 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    22861 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    22921 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    22981 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    23041 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    23101 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    23161 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    23221 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    23281 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    23341 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    23401 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    23461 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    23521 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    23581 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    23641 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    23701 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    23761 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    23821 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    23881 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    23941 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    24001 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    24061 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    24121 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    24181 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    24241 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    24301 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    24361 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    24421 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    24481 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    24541 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    24601 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    24661 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    24721 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    24781 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    24841 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    24901 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    24961 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    25021 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    25081 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    25141 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    25201 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    25261 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    25321 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    25381 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    25441 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    25501 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    25561 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    25621 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    25681 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    25741 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    25801 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    25861 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    25921 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    25981 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    26041 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    26101 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    26161 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    26221 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    26281 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    26341 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    26401 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    26461 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    26521 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    26581 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    26641 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    26701 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    26761 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    26821 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    26881 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    26941 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    27001 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    27061 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    27121 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    27181 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    27241 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    27301 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    27361 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    27421 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    27481 aaagatccaa tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat
    27541 aattccagta aattactata attataaaaa aagaattgac aaaacataac tgtctatata
    27601 aaattgatta cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa
    27661 tctctgagga acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat
    27721 caactggtct agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa
    27781 aaagatcttt aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat
    27841 tggaaaaggg attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga
    27901 acggatattt gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat
    27961 tgatgaacgc tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat
    28021 ggaaaagttg aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga
    28081 gaacattaat aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta
    28141 cagtgatgaa ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat
    28201 tccatatact tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg
    28261 tcggaacaat aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt
    28321 agtggaatca gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag
    28381 actggtgaat catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt
    28441 agacacggaa atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa
    28501 aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg
    28561 ttacttggcc atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa
    28621 tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt
    28681 ccagttgcgg tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct
    28741 tatagtaacg acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa
    28801 ttacctttgc tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct
    28861 gctgccttgg caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca
    28921 gtagctactt tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt
    28981 aacgtattta ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac
    29041 gaagcaaaat taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg
    29101 gctgcctttg tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac
    29161 tctgctttag tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt
    29221 tatggcttct tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg
    29281 ttctggtttg acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca
    29341 aaaggcgtaa caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca
    29401 gcaggagcat atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc
    29461 ttgatgttag cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc
    29521 tcatttatgt tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct
    29581 ttagcagtga gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc
    29641 gattacttcc tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa
    29701 ggattagtaa cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat
    29761 ctgatgactc ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat
    29821 tcagacaata aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac
    29881 aatgtaactt cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc
    29941 ctgatcgatc aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga
    30001 aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa
    30061 ctacatggtg gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca
    30121 tttgccggtg gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca
    30181 atcactgaag tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta
    30241 ttaccagaaa atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca
    30301 acgaaacatg ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc
    30361 atcaacactg ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa
    30421 gtcgcaagag gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa
    30481 aacacggata tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa
    30541 cccgcacgtg atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc
    30601 atcgtcttcc ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc
    30661 tcagctcctt tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca
    30721 gccaaacgta acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga
    30781 acagcttttt tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc
    30841 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttcata ctcaaaacta
    30901 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    30961 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    31021 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    31081 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    31141 ttttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    31201 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    31261 attcacgttt caatacccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    31321 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    31381 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    31441 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    31501 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    31561 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    31621 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    31681 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    31741 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    31801 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    31861 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    31921 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    31981 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    32041 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    32101 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    32161 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    32221 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    32281 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    32341 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    32401 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    32461 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    32521 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    32581 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    32641 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    32701 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    32761 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgattacaa
    32821 caagatacgt tccttttggg aatgggatga ttcgggtagt gtttggaaca tcctgactcg
    32881 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    32941 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    33001 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    33061 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    33121 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    33181 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    33241 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    33301 cttcgactaa ctcataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    33361 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    33421 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    33481 gcatcaaact gcccttttct tccaagcggg ctccatcaaa tccataagaa acgttgatta
    33541 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    33601 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    33661 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    33721 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctgctga
    33781 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    33841 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctaggaga tcgcgattag
    33901 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    33961 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    34021 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    34081 ttatcttcct ccttgcttgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    34141 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    34201 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    34261 ataaattttg gggaagacac attctaagta aacaaagcgg taaaaatatc ttttattccc
    34321 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    34381 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    34441 tttatttcta acaaaatcac ttcat
//
LOCUS       DAZKOK010000015        31124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000015 DAZKOK010000000
VERSION     DAZKOK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.015"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..164
                     /locus_tag="ACG90W_000709"
     CDS             <1..164
                     /locus_tag="ACG90W_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFQ0477097.1"
                     /translation="QEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILK
                     AVTGLNIET"
     gene            276..1679
                     /locus_tag="ACG90W_000710"
     CDS             276..1679
                     /locus_tag="ACG90W_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0477098.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            2028..2660
                     /locus_tag="ACG90W_000711"
     CDS             2028..2660
                     /locus_tag="ACG90W_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0477099.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3248..4687
                     /locus_tag="ACG90W_000712"
     CDS             3248..4687
                     /locus_tag="ACG90W_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0477100.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4982..5200
                     /locus_tag="ACG90W_000713"
                     /pseudo
     CDS             4982..5200
                     /locus_tag="ACG90W_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5449..5664
                     /locus_tag="ACG90W_000714"
     CDS             5449..5664
                     /locus_tag="ACG90W_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477101.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5881..6137)
                     /locus_tag="ACG90W_000715"
                     /pseudo
     CDS             complement(5881..6137)
                     /locus_tag="ACG90W_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6324..7235)
                     /locus_tag="ACG90W_000716"
     CDS             complement(6324..7235)
                     /locus_tag="ACG90W_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0477102.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7254..8258)
                     /locus_tag="ACG90W_000717"
     CDS             complement(7254..8258)
                     /locus_tag="ACG90W_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0477103.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8255..10378)
                     /locus_tag="ACG90W_000718"
     CDS             complement(8255..10378)
                     /locus_tag="ACG90W_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0477104.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10383..12830)
                     /locus_tag="ACG90W_000719"
     CDS             complement(10383..12830)
                     /locus_tag="ACG90W_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0477105.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12817..13206)
                     /locus_tag="ACG90W_000720"
     CDS             complement(12817..13206)
                     /locus_tag="ACG90W_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0477106.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13266..13769)
                     /locus_tag="ACG90W_000721"
     CDS             complement(13266..13769)
                     /locus_tag="ACG90W_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0477107.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13782..14006)
                     /locus_tag="ACG90W_000722"
     CDS             complement(13782..14006)
                     /locus_tag="ACG90W_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477108.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14110..16020)
                     /gene="ltrA"
                     /locus_tag="ACG90W_000723"
     CDS             complement(14110..16020)
                     /gene="ltrA"
                     /locus_tag="ACG90W_000723"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0477109.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16766..16903)
                     /locus_tag="ACG90W_000724"
     CDS             complement(16766..16903)
                     /locus_tag="ACG90W_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0477110.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16900..18084)
                     /gene="mobT"
                     /locus_tag="ACG90W_000725"
     CDS             complement(16900..18084)
                     /gene="mobT"
                     /locus_tag="ACG90W_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0477111.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18343..18633)
                     /locus_tag="ACG90W_000726"
     CDS             complement(18343..18633)
                     /locus_tag="ACG90W_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477112.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18623..19765)
                     /gene="dcm"
                     /locus_tag="ACG90W_000727"
                     /pseudo
     CDS             complement(18623..19765)
                     /gene="dcm"
                     /locus_tag="ACG90W_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19841..20005)
                     /locus_tag="ACG90W_000728"
     CDS             complement(19841..20005)
                     /locus_tag="ACG90W_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477113.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19998..20672)
                     /locus_tag="ACG90W_000729"
     CDS             complement(19998..20672)
                     /locus_tag="ACG90W_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0477114.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20750..22090)
                     /locus_tag="ACG90W_000730"
     CDS             complement(20750..22090)
                     /locus_tag="ACG90W_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0477115.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22173..22508)
                     /locus_tag="ACG90W_000731"
     CDS             complement(22173..22508)
                     /locus_tag="ACG90W_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0477116.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22518..22892)
                     /locus_tag="ACG90W_000732"
     CDS             complement(22518..22892)
                     /locus_tag="ACG90W_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0477117.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22905..23219)
                     /locus_tag="ACG90W_000733"
     CDS             complement(22905..23219)
                     /locus_tag="ACG90W_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0477118.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23332..26559)
                     /gene="fss3"
                     /locus_tag="ACG90W_000734"
     CDS             complement(23332..26559)
                     /gene="fss3"
                     /locus_tag="ACG90W_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0477119.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27416..27670
                     /locus_tag="ACG90W_000735"
     CDS             27416..27670
                     /locus_tag="ACG90W_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477120.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            27834..28751
                     /locus_tag="ACG90W_000736"
     CDS             27834..28751
                     /locus_tag="ACG90W_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0477121.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28744..29526
                     /locus_tag="ACG90W_000737"
     CDS             28744..29526
                     /locus_tag="ACG90W_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0477122.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29527..30207
                     /locus_tag="ACG90W_000738"
     CDS             29527..30207
                     /locus_tag="ACG90W_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0477123.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30200..31096
                     /locus_tag="ACG90W_000739"
     CDS             30200..31096
                     /locus_tag="ACG90W_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0477124.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
BASE COUNT         9155 a         6318 c         5602 g        10049 t
ORIGIN      
        1 gtcaagaaga tttatctgga ctattatcaa tgttttctct tgctatagaa gaacaaatta
       61 cattagacaa attgaaaatg ttagattatt tatttcatcc tagttttagc caaccctata
      121 attttatttt aaaggcagta acaggattaa acattgaaac ataatcgtta ctaaatgaaa
      181 tcaagaaata tactgtatta ctcaattcat tgaaaatatt tatcataatt tttaatagaa
      241 aacactatta aagagaaaaa agagaggtaa atcaaatgaa aaaatcttta aaacttgcat
      301 cagcagttat attaatgtcc caactcctta tagcaacacc tttccatgtg ttggcaagcg
      361 aaaattcaac atacaaaatt gcaaaagaaa gaaccgagac gactgaaatg agtaattccg
      421 atgtaacagc agagagtagt gaacttttga caacttcatc aagtgaaagt agtacgactt
      481 caaatgccga gtcttcaaca acttcatcaa gtgaaagtag tacgacttca aatgccgagt
      541 cttcaacaac ttcgtcaagt gaaagtagta cgatttcaag taccgaatct tcaacaactt
      601 caagtagtac aagtgaaata acttcttcga aagtgacaaa accaaaccat atagaatctt
      661 caaaaaagga agcgccaaaa acggaaggac aaacaaaaac tttttcgaca ccacagcaaa
      721 tagaatcaac agaatctttc caatcacaac caatactatc tggtaatgaa gaaattcatt
      781 ttgacaaaaa tgaatctgta gaaaatttta ttatgaaaat tggtgagtct gcgagaaaaa
      841 ttggtcagaa atatgattta tatgcttctg ttatgattgc acaagcgatc ttagaatctg
      901 ccagtggtca aagccagtta gctcaggcac cgaattacaa tttatttggg ataaaaggaa
      961 cttataatgg aaactttgtc attatggtaa caaacgaaga tttgggtaat ggaacgcttt
     1021 atacgaccca atcaaaattt agagtgtatg aaaactatga ggaaagcttt gaagattatg
     1081 ccaaattatt aactaaggga attagtggaa ataaagattt ttatgcagga gcattaaaag
     1141 ctaatagtaa aacatatcga gaagcaacta aattccttac aggacggtat gcaacagata
     1201 cacaatacta tttgaagcta aatgaattaa ttaaaaccta cgatttaacc aactatgata
     1261 aagaagttaa aggtccacaa atgagtaaga ctggatacca agtgcctatt cataattatt
     1321 caataaccag tttgtttgga aatcgtggaa atgaatttca ccgaggactt gatatggcag
     1381 ctacacaggg tgagccgatt catgcagtta aagctggtaa ggttgttaaa gcagagtttc
     1441 actactcttg gggaaattac gtggtgatac aacacgaaga tggatcaacc gcgttatatg
     1501 ctcaccagca acaataccta gtaaaaactg gagataatgt tttgcaagga caaataattg
     1561 gctatgtagg aagtactggg aatagtactg gcccacacct tcatttggaa atttgcaaag
     1621 atagtagtct ttctcagagc atgttagttg atccgaaaac cttattattc ggagactaaa
     1681 aaaggtaatc aatctgacaa agtaacaaga aaatggttgg attcttagac ctttgatcag
     1741 gatattgaga ctggaattga tatctcgatt acgatgggtt tgacaaataa aacaagtcca
     1801 ttatcgaata tcgagtgtat tattgccgtc tttatgctca tattttgagc aggtttcact
     1861 agatgaaaac aatttatcta tcttgaggat ttcgtgtacg tatcgttcag tcttgtattg
     1921 taaattagtc ataaaatcag actaagaaaa attggaaata tttttttctg atttattaaa
     1981 agttaattga aattatatgg tacttctttc ttgggagggt aggtgcagtg aaggaaagat
     2041 atggtcacct tactgttgtt gaggagatta ctcataatgg atataaaaaa tatcgttgcc
     2101 tatgtgactg tgggaatgaa acgattgtat atcgatcaaa cttactatca ggtagaacca
     2161 aaagttgtgg atgtggaaat tataaaaata ggcataaata taaaaatttg acaaataaaa
     2221 cttttggtaa gttagttgca atcaagccaa ctaaactgcg aagtgaagga actgttgttt
     2281 gggaatgtca atgtctgtgt gggagaaaaa agttagtatc ggcaaagcga cttctaaaag
     2341 gtgatgtaaa aagctgtgga tgtttgaaat caaaaaagca tcacattgaa aataaaaaat
     2401 tcggattact tactgcaaag tatcctttgg acaataactg ttcatggaaa acaaaatggg
     2461 tttgtcattg tgagtgtggc agtatgtgtg agataagtta tagtaactta ttttttggac
     2521 atactcaaag ttgtggatgt ttaaaaaggc aagaaacaag gacatgtgtt gaagggacag
     2581 taatagagtg tctaacgtca aaattaccga aaaataatac aagtggtgtt aagggagttt
     2641 atcgttctaa agagaaatag attgcgtata taactttaaa aaaaaagagg tactatttag
     2701 gatcgtatga ttctattaag agtgcagctc aagctagaaa agatgctgaa aataaattgt
     2761 ttcaaccttt gattgacaaa tatcgaggta atcattaatt attgtggata ataattaaca
     2821 gtgatatata aacatctata atatattatt catactattg atatttagta taatcctatg
     2881 gaattgaata tcatagcaat gtatttgaaa agcaaattgg aaaacaaata ggtgcaacac
     2941 cataaatatg aaaaaattgg gaaaaagtag aatggaaatt tgcttacgtc tcacgagata
     3001 gctcacacta ttaatctgaa gaacaatgaa gtcgactttt agaaattgct agaagaaagt
     3061 aaagcgaatt atcactagtt actatagagt aagttcaaat aaagcatctc caacttacat
     3121 ggttacatga ggatatcaaa tcgaaattac tcaagcttcc tatggctaga gtcgcgagaa
     3181 gttgttgggc agtagacata ggttatattg ttcttagcta cacgatcatt agaaaggaaa
     3241 tcctattatg tatagttatc aagaagatgc aaaagtccaa aaaaatcgct ggtggatttt
     3301 gattgctata tctatgttta ccttcatgtc cacattggat ggcagtattg tcaatattgc
     3361 actacctacc atctcagatg atatgggcat accaatgaat caagcggaat gggtagtgtc
     3421 cgtttatctg atgattgtct gttcttgttt gttgctgttt ggtaaaattg gcgatagccg
     3481 tgggaaaatc aaagtatttc ggattggtac gctgatcttt gtcttgggat cgctgttgtg
     3541 tgggttcaat cattcgctag gtattttatt gttcgcacgg gtggtacaag ccattggtgc
     3601 tagtatgact atggctacaa atgccggaat catcacggaa gtctttccca tacatgaacg
     3661 tggtcgggca ttgggagcaa taggtgcgtt tgtttcatta ggctcgattg ctggaccagg
     3721 tcttggcggc ttgattttgt cccaattttc ttgggcgtac attttttgga tcaatgtacc
     3781 ggtgggaatc gtaacgatgt tgattgctga aaaattctta ccaaaagata tcaccatgag
     3841 cggccataca atcgatggag ggggctttgc tgccattgcg gctacgatta tcagcttttt
     3901 tggagcaatc tttttggggc aagaaagtgg ctttttccat ccagtccccc ttgtactgtt
     3961 cgtaatttca gtgattgcct ttgtcagctt tttaaaagtt gaaaagaaag cagcctctcc
     4021 attgatttct ttcggtattt ttaagaacaa gatttttacc atgagcttga ttacagcctt
     4081 gttgattttt tcttccaact tctttgtcaa tgtagtgatt cctttttatc tgcaaaatgc
     4141 tcgcgggttg agtcctagct atgctgggct gttgatgatg gtctttcctt tgatgatggt
     4201 cgtcggggcg cctttaagcg gctacctgac cgaccgggtt ggcccggaga ttttggtatt
     4261 agtaggcttg ggcttattgt cggtaaccca ggtcatgtat atgtttttgt cagtggactc
     4321 tcctttgtgg ctatacattg tcgctactgc catcatgggt gccggcaatg ccttgttcca
     4381 gtcccccaat aatacgatgg ttatgagctc ggtcactatc gaaaacttgg gactggcagg
     4441 ctcgatgaat tcctttgccc gaaatttggg gatggtcagc gggatttcgc tttctacgac
     4501 ggtcttatat caagcgatgt ctgctaagat gggccagcaa gtaactaact atatcgcaaa
     4561 tcggccggat gtctttttat atggaatgaa gattaccttt ttaggcagct ttgttttgtg
     4621 ttttgttgcc tttttcctga ctatctggcg tatcaaaaaa caaggaggta ttcagaaaaa
     4681 tgtctgaaaa aaccactgtt gaagaatttt tggaggcctg cgtatgggtg agtgggattt
     4741 ccatggatga tggaagatga agtgaacttc taaaagccga aaaactcaag tattatcttc
     4801 aaaatactag tgctcaatga gatgcttgat ttgatcacaa gaagactgcg aaagccgctg
     4861 gcaaccgcaa gctaggtatc ccaccgatgg actacgtttt agaggctact ggttatgaat
     4921 acggagccaa cgctctcatc tgcataagaa tacatcaccc agattatgta aagattttcg
     4981 atacttctgt caaaaaatca ttaagataga taaatgattt ccgtctagtc aaattggttc
     5041 agaatgtaga cacacagatg gtaagaagtc actcacaatt cgagaatgga cttgtcctat
     5101 ttgccgtata catcataatc gaaacgtcaa cgatagtatc aatattttga ccaagggcta
     5161 tgaatacaag cattgcctta gaaaacatca agaaccttag gaacaacggg gatagcttgg
     5221 ttaataagag ataccgctgt ttaggcttgc aagtatactc ttttatcaag aattccgtgg
     5281 ctttagtcat gagaggctta atactatgaa aattttcttt agcatttttg atttaggttc
     5341 cttttctaat tataataaga tgaccactac agtaacctac tgaaatatat aaacagccac
     5401 tgtagtacca ttttcaaagt agtctatgag aggagaatct tcattcttat gaacgctgga
     5461 attgcggata tgaatttaat aaaaaaaacc cttaatgatt ttacttccaa ttcaatatca
     5521 aaaggtactg gtatcaattt aagcacaata aaaaagctga aaagtggtga aagatcagtg
     5581 gaaaagctga atcttttaga tgctataaaa ataacggaat ttgctatgaa aaatggaaaa
     5641 gctgagattg aaatatggag ataattatcg tctagtgaat tacttgccac ttagctgaat
     5701 ttaacgatcc ttggtgcttg aagggggaga ttcttgggaa tagagcatac ttgcaagcct
     5761 atgtctttac taacaacgat atctcttatt aaccaagcta tccccgtagt tctaaggctt
     5821 ttacttttat ctaagcaata atcgtattca aaggaaattt ttgaccctta gaaagtctca
     5881 ttttatcaga cgtgagctta ggcattgctg aattttgtta attcggtttt aggttgtgct
     5941 ttgagcataa tatgaaagta ttctttatca cgatttcatt catctaaggt aatgtgatgt
     6001 gattctgaaa ttctttcaaa ggcagttttt gcataagctg acattacatc atcaatgaca
     6061 tgtttacaat gtttcactac taaaataagg gggtaaccaa gaagaaaaac tgaatgatta
     6121 tttgtatcta attccatggt atatcaacca tttcctatat agactgatta tgacatggat
     6181 taaaataaaa aggcactatg tcttaattgt cggttttcga gttaccgaat ggcattcatc
     6241 acccacctat agaggatggg cgacttctgc ctgaaataag ttacactact tataacttct
     6301 aataaaatta gattgagtca ttattattta acatcaacga ttttccagtt tccatttttt
     6361 tgtaatacta atttgtactg aaagtagcta gtggtttttg atacattatc aaggtatttt
     6421 atctttgtac tcactagaaa attttttccg ttttgtagat aaactggatt ctctaactcc
     6481 gaaaacttta agtcaatatt gattggtcgc atcgcaccat tttcaacata atactccaat
     6541 tctttttcag aagctgatgg ataaagttta aaaaatgttt ctaaaaaatc ggtgatttct
     6601 tccgttgttt tggcatcaat ttctgaatcg ttttctactt gttttgcttc atattttgcc
     6661 ttgtctggct ttccaatcat tgttggcaaa ctagttacta aatgattcct gttttcatct
     6721 tcaaagattg tcactcgata tgcagaagaa attgattgcg tcttttttcc ctctgtaata
     6781 tgttgctcca ctaaaaatga aacgacaaat tcttccgagt gtttttcaac atctagaatt
     6841 tttacagatt gaacttcgga actcgtggga atatctgctc gaaccatatc ttggattaaa
     6901 gcgagaccgt tttctgtaag gtactgttcg agattactca ttctcttttc aattgcttct
     6961 ttgttatttt tccatgaaaa atattctttc gcaaaatcct ctgtaaaact ttcaacgcca
     7021 gaagtattca ctaccttctc ttttataacc actttttcat gagttgtatg ttgatcaatg
     7081 gcggtgaaat ttttataaac gccaaaagca agactacaag acaataaaag ccaaagggca
     7141 agcaccattt ttcgatgctt acccacggat acaactcgtt cttttttctt ttttgggctc
     7201 ttttcctttt gattccgttc aatttttatc ttcattcttt ttcctctttt ctcttatttg
     7261 tatcggccag ctccgataag atgctgccgc caatagtttt ccgttagatt cgtccacccc
     7321 actggatctc ctgcatgaaa catgcgattc tcccctgcgt aaatgcccac atgggtcaca
     7381 taagtaccgg catcataggt tgaatgaaag aaaactaaat cacctggttt ggcatctttt
     7441 agatcaatat gagtcatggc atcatattga tcctgagcca cccgaggaag tttcaggtca
     7501 actttggcat aacaccattg ggtcaagcca ctacaatcaa accccgtttc tggacttgag
     7561 ccaccaaaca cataggccgt tccttcatac ttaaatgcct catcaaaaat ttcttggact
     7621 attttgtcat caaactgagt tgtagttagg tattgcttca ctaataaaca gtaaaacata
     7681 ttgccataat tataccgcca cccaccattt tccttaatcg cgattgggtt ggggtaatcg
     7741 actttttgac cacctgattt ttcctttgaa aaagattcag ctaaagcaaa actatgcttc
     7801 ttcccgtttt tcccgacatg atctagatac ccgccaccat agttataggc ctgaataata
     7861 ctatcttgat cacaccctag ttctttggaa cgcttaacca aagacgcaaa atagagacag
     7921 ccttgtttga tggatgcctc ggtacttaat gaattaggtg gtaatccggc tgactcagaa
     7981 ctttgcataa cgtcttccat tgtgccacca gattccactt gaataatcgc caaaagaatt
     8041 ggaatatact ctgttatatc cgattctttg gcatactttt ccaccattgc acggtgtttc
     8101 aatacttctt ctgagacact agttccgcca ggagaagtgc cagaactacc atttccctca
     8161 ctgtcttcac caaacaacaa acccacacac atcaaaatac ccataaaaga gatggtcaga
     8221 aaagttaatg aaataacacc cactttcttc aagttcattt tttctcacct gaccttgatt
     8281 ttggacgatg gttcattctt ttttgtactt tttgattggt ctgctttggc agagtggacc
     8341 tctgtttcac ttgttggaaa gaatttttct tacttgctga ttggcttttc ccttttgaaa
     8401 tgatttcttg actagttagt tttcgtttga tttcgggatt cagatttgct ctcacaatcc
     8461 gctttggtgc ttcttttttc agcatcatat ctttctgagg ttgagctgct ttatctattg
     8521 gaatattcgc ccttcttttt ccctgacgat ttgttgcttc actcagtatc ttgcgttttt
     8581 cgtccatttt cgcatttctt cgttgtcgtt gtttttctcg caaatccact cgatccgctt
     8641 tttcttggaa cagtccgcgc ttgaattctt ctggtgcttt tttcgacttt tcgattcctt
     8701 tatggagatt gtatttcaaa ttcgttggcg tattccttac ctgatccttt acttgtttag
     8761 tcttatcttt caccaaagct tgggtatcca aaactttgcc gatttttctc ccagccaaat
     8821 tcattcggga ttgtttctta ttatttgaaa ctggattctc cttagttaag tcagatagta
     8881 tttcgtgatc gttcggtaat cgtgaattct ttcgtagaga agaaccggat ggcttaaatt
     8941 tggattttga ttgttccttt gccaacttat ttccgactgc tgcaccacca ccaaaagcca
     9001 aagctcgtcc cacatttcga ttgagttgcc gcaattttcg attcattaac atttgcggtt
     9061 ttcgcatgac gcgtcttccc attgactggc tatcattact ctggaggttg aacatactca
     9121 taatgtcgcc caatttgaaa tagataccgg caaaggtcac aatttgcaga aaagcaacca
     9181 tgaaaaatgg ataggttgcc gaaatattga aaaacatcgt ggagatgcta aatgcagtcg
     9241 taatcaccaa agtaacaccc gctcgcatca taatcgtatt gaataaacga ataatcgcct
     9301 tctttcctaa actttcataa gtcggcaaca tggacaataa gaaactaatt ggcagaaaca
     9361 tcgcaaagat aatgaagaga atttgcgaga acaacataat ccctgttaga agaaaaacaa
     9421 agaaggaaat gccaatattg aacaatacca aaaagaccac ggtccctaaa cggttcatgg
     9481 tcttggtaat actgagattc gcattgtcat tgtcttcaat ctctgccttg accgcttctt
     9541 cccgatcttt gcctttgttt tcatctggac tcacagatag aatcttgttg acccggtctt
     9601 caccaatttc ttctatgttg gaatcatcaa attgcagtag taaccaaggt tgttggactt
     9661 gaatcgaaaa caaactatcc cgaatcaaat ccacactatc ttttccttgg ctttctgaat
     9721 tgggtacaac gatctcggtt ccaagggtaa gggccgcttc actaacatct gagctaaagt
     9781 cattgatttt tgtgatatag gtaggcgcat aagcaatgaa cgaacccgat agtagaaaaa
     9841 tcatcacaaa attaatcacg gctcgaactg ctttcgtggt ttctcgtttt aataatccgg
     9901 tatacgccac atacaccccc agagctaaaa tcatcaataa tagaaaccca aaataaaatc
     9961 cggaggtctg gagtccattt tcagtaatac cagccaatgt ttggatattc tttccaatac
    10021 tttcagccgt atccgaaatg aaatctaatt tgtaagcctc ttgaaccaca taacccgtgg
    10081 cgttcgacaa atacaaactc accgtccaaa tgaaattagt aatggcataa agaccataca
    10141 tcacgctttt ccctaagcca tcaccccaat tccaaggcaa ccaatcccaa gagctatcca
    10201 caaagaaatc tagttgatag ttgttcagtg aatatttgga atatagattt cctgcttcaa
    10261 ctgtatcatc aactagccct gtcgcctcgg ctaccgtacc aactaaactc aatagaacta
    10321 tcagagtgag tatgctaata aaaacgattc ccacaatccg aaaaagtttc tttttcatcc
    10381 cctcactcca ttctctcttg aacaggtgga cgggtatcaa aggcatggaa gagttcttca
    10441 aagactggat gtacctgaac cacgcctacc cgaccataca aatcctgcat caagcattgg
    10501 ccattttcca aatctcgtag gcgtttttga ttgctttcat cttctcgatc caagccaaag
    10561 aattccaagg ttttcttgat ttcatgaata tcggtactgc gaaacgcaaa cttcaacccg
    10621 atattattct tcaagcgttc atccagtaaa tcatcactat tttgcgtgac aaagtaaacc
    10681 ccggcattca tagcccgccc agcacgaacg agcttattcg ataatgtttt cccttgcgct
    10741 acttgaagaa aactccacgc ctcatctaag tctacaattt tgaagatttc tcgattacta
    10801 tggataaagt ccaaggcaaa agtggaaatg acgattagca tggaaacact aagcaattcc
    10861 atggtggtat attcttcaaa agtggtttct gcatctggta aaaccaaatc cgctacttga
    10921 atgatattta actgcctatc taaactaatc gactgggtaa tcgaaccatc tgagaaaagc
    10981 aaatgggcaa aatcataatc ggtaaaggac tcgatgtgat ccgcgatatt ttccgcaact
    11041 ggtgagcctt cttttcgaag ctcattaatt acttgtaata atccccgttg attgctttgg
    11101 gtaaccgtcc gaatggcctt acgcaacact ggaaacttct ccccatctcg actagaaatt
    11161 cctgttaaaa aggtcagaat atcaattgct agactttcag aatccttctt tttctccata
    11221 atcacataag gatcaagtaa tccttgattt tttggcttac tagttaagtt gagaatattg
    11281 atttcatccg caatttctgg caaggtttct ttccagccac cccgttcccc ttttggatcg
    11341 acaatcacag cttgtccacc aaaaaggacg gcgtaataaa ctaagagatt gttactaaag
    11401 gactttccgc caccgagtga accgaggaat gccgctgcta gcgcattggt aactgaatcc
    11461 ttgacccctt gtgaggccaa tgccggtttc aggtaaacat tgcgccccgt atctacgtta
    11521 taaccaaagt aaatgccttc caactcccct aacatctgag tggcgccaaa accaagtccg
    11581 gcgagaaaat cagacgtaac atactggata taatcattca tgtatcgctt actagaaggt
    11641 aagaattctt catgtagccc catcatatcg ccaaaaggcc ggactagttt tacattggta
    11701 tcatcataaa aatctagcac ttcatcacac cggcgcttta gttcatctac tgattcggca
    11761 cttactcgaa ccacatagct cagtttgtac atggattctt tggactgatc caaggtagtt
    11821 tccaattcat ctacggaatc caatgcatct aagacattgg aatttgtttc attgtcagat
    11881 tgataggcat ggttgtccaa gtcttttaat tcctttttct tattgcgaac ggtagcaagt
    11941 gcctttttgt tcgtcacaat ctccacattc atactagtgt caattgggaa tgtaaattgt
    12001 tgttgctggt aatagaaaag ttctgaggag ggaaactcca tttcccccac aattgtatta
    12061 attgttaagt acgctacata tgattcatga ttctcatgtt ccatgcataa gtaccggggc
    12121 ttttcttcaa tcaaacaacg actgggacgt aataagtcgt aacgtttcac caaagtttcc
    12181 gacttcagct ttttctttgg tagctgaaat tcatattctt caaatggtgt acccttttcg
    12241 ccatagatat gttcaatcaa atacatcaga tcactcggtg tgactcgtct gactttaaat
    12301 cttcgagcca atttactttc cattagtttt tcaagcttgg tatatcggcg aatttcttca
    12361 ttggataagg aaacaaaatc gcccatcaaa tgatgattca ctccatagac aaattcttga
    12421 aagccggaga agaaagattt cttgagattc tttagattga cctcttcatc tgtcgcaatc
    12481 agtttgaagc caataaaaaa gcgataatca atctgactat cgccaatcat ggatactaat
    12541 gcctctgtct gtaaatcaat tcgttgtttc gctacttctg ttaatcgccc ggtaatttcc
    12601 ttttttgatc gttcttgcgt ggcacgaata ctactttctg tggcaatttg taaggcatga
    12661 atcttgcctt cacgattttg cgcaatcaat tgacgaaagt tatcgtggac ttgatatttc
    12721 tgttcaggtg acaaaaagga gtaattgtaa ggtattaact catagtaggc aaaacattct
    12781 ccctcttgat taaagacaag attattttca atatacttaa tcgggtacat agttcacact
    12841 ccttaccaga gtaatgggtt cgtcccatcg tttcttctgt tctttgactt tctttcctgc
    12901 gaaagtgacc ttaaatcgta agtgatagct aataaaagat cgtaaaaatc ccaatggttt
    12961 cttaccgtca aacgtttttt gcgacatgaa ccaagtaact gccaccggta tacctaagta
    13021 ttttaaaaag gccccctcta tcatagatag tggaggcaca tgggcaaata cgatgacaaa
    13081 cagcaaggat aaaacaaacc aagccatctg gttaaaagtc acaggaaaag gcagttgaaa
    13141 gtcattaatt gcataaatga ccttttcaac tgcccaaata ctggtgtagc tttttatttt
    13201 cttcatcaaa tcattccttt aaaaagaagg atagtttaga aatcaactac ccttcaataa
    13261 ttttgttaat atggatactc aaaaacacct cgactcgtta ccaaataatt gcctccaatc
    13321 tccaagtctc taccacacgc atcaaaatca atatacgtct tcaagtgttc tggtacttct
    13381 ccaaaatcac cagcttctac aatcaagaaa tatgctacat cactcatatc atcgcaatct
    13441 ggataataga taatatcatc cacatgctct accatctcct caaaagaatt gaagaaagca
    13501 tgttgaatct cagaagcaac ttcaccaact ggcgttcctt caagttcctc cgctaagcca
    13561 cacaaccgat taatttcctc aattggcgta tactcatcaa tctcaaaggg aagttcataa
    13621 tcatgaattg catattcctc gtattcgtca tttaacccaa ttcgttcttt cacttcatca
    13681 aaatcgacag gtggggtaaa ccacgccccc accaattcac cttcgttata tttcccgagg
    13741 ttcgcaatat aaacacgcat ctgttccatc gtgcttcccc cttacgcacc gacaattcgg
    13801 ttaaacaaac tgagtaagac atccttcacc ccagaagcat taaagaccaa tccgaccgca
    13861 atcaaagcaa tcaccaaaaa gccaatcagt ttggaaaact cccgcttaaa tcctaaaaac
    13921 aaaccaatga ccacaattgc cagtaagact aaggattggg cattggttaa aaaccattga
    13981 tatatggata ggtaagttcc tgatattata aatatctcaa acttttcccc ccattcacac
    14041 ggagcgtgcg actttcaccg cactccgcgt tccatcgtgt ttatattgaa aagtatttat
    14101 gtagtagacc tatatcaagt ttaattcttg attgccagct aatactctat atttatttag
    14161 tcttatgagc tggactttat ttagcttcaa tagagctata tatttttgta ttgtttcttc
    14221 tgttttagcg tgtattagac ggtgtaccgc ctcttttacc aaaataagat ttttataatc
    14281 gtcatttcca ccagctccag ttggtacaat atgatgacaa tgtatttcat tagcttgttg
    14341 aaattcttcc agagttatag cacattttcc atgttgagca gaaaatagcg aaattcgatt
    14401 gtctgcgtat tctattgtgc gattttccaa agataatttc attaactggt acatcagtaa
    14461 tttatcaaca cttagattgg tgtggataaa ttttcttccc tctagagtgt agcaattcgc
    14521 taaatacacc tttgccattg gttttctgtg ttggatatat ccaacaggga aaataggttc
    14581 gccctcgcca gcaacatacc gaagcatttt agatttacca tatcttgttg cctcctttgt
    14641 cgtaagttgt ctgcctgttt ttctcaatct gccccttttg tttgtaccta atctgttagt
    14701 gaagatagcc attacagctc tattcagttt catacaatcc agatttacat tcgttgcaat
    14761 ctgataataa ttttgcatac ctaataccat tgaattataa agtcttgttt cttctaattc
    14821 cgttttccct tttgagggag aagcaatttt ctttgcctgt gataccagct tgtctttttg
    14881 gtttttgaat tgtttatcac ttatatgtga tttaacaact aacttctttc ttctcggtat
    14941 cattttaatc ttgaaaccaa gaaagtcgga gtatcttttt ctaacattta caattcttgt
    15001 tttttcttgt gatacttcca attttaatcg ttgttcaatc cattgagtga ttgctatttt
    15061 ggctttttca gcactttcct tgtagcggca gaaaactcta aaatcatctg cataacgaac
    15121 catatacatt tcctttaact ttgttttctt catttctttg taagcgttgc ttttacatgg
    15181 gctaccgctt ttattgatat gaaccacata gttttttgta acaggatttt cctgccattg
    15241 gctttctacc caatggtcta actcattaag aacaatgttt gctagtagtg gggatattat
    15301 tcccccttgc ggagttcctt tctctggata tgttattgta ccgttagtca ttttgattgg
    15361 agcttttaat atccttttta aaatgaatat caagtgcttg tcccgtattc ccatagccca
    15421 tatctgtttt atcagcttgg aatggttcac gttgtcaaag aaacccttaa tatcaaattc
    15481 aatcacataa tgtagctggc tgatttgtag gcggttatag gttgctttta ttgcattttc
    15541 aactgaccta ttaggtctaa aaccatagct gttttcacta aatttagctt cgcagattgg
    15601 ttctaatact tgttttatac attgttgtat aagcctgtcc cacatacaag gaatacctag
    15661 aggtctggtt ttaccatttg gttttggtat ttcttttcgc ctcactggct taggtcgata
    15721 cccatgtgga ctgcctgcaa caatgtatct tacttttttg acaactgttt ctgctggcaa
    15781 tttgccaata tctttaatta ttgtaccgtc tgttcctgct gttttactgc ctgcatttgc
    15841 tttgatattt cgatatgcca gtaatatatt ttctctcgat aggataatat ccataagatg
    15901 agtgaaaata tctccattct tacttttggc atataaatca tcaaaggtgt tttgcatttc
    15961 gtaatattca gcgtgtctta aatcttctac acacaatgtt tcatttttat ttttaggcat
    16021 aaggcttcac tcctttactt gaaattgagt gagctttttt atcttactcg taatccttat
    16081 tgaaacaaca taatactttt ctaatatata cacgacttga gggcattcct ccattctcat
    16141 tacaagaatt tcttaggttc gccctctgct tttcagcatt agttcaactg cttattgttc
    16201 ttcgtcagtt tattctcttg atgagaaccc aatacctttc ctcgttccga taatcttatc
    16261 tatacatata tatccttagg tcactagcta taagcctgct ggcttgatac cgcctgtaac
    16321 gttatatggc atttcataac ctatattctt actttgccac accagctacg atttctcgta
    16381 cctttgcatt tctacaaagt tcaaatttag acccgtacat tcgctgtttc gtcagttcct
    16441 tatagaacat tctaaccata gatattttat agacccccga cctgttggat acgctaccca
    16501 cctctggata gttttcgata gaagatatat gttcatcaac tattacggta tctttcagcc
    16561 gtctttaccg agcttcaaac attctgagtt tcctcataca atgcttgtcg gaggctcagg
    16621 gcaggcgttt cagagcgtta cttcatcatt ccacctgtca gattatcagt tctcctaata
    16681 accaaacttt ttcgttcttt tattagtgcc ttagcttttc actaaggaac gagtcgcaca
    16741 taaattttgt ccgaaattca tcttgtcact ttcctttctt ttccattgct cgatccgctg
    16801 ctgcacttgc attcatcaag cacatcacag taacaccaag catactgcca ccaaatacca
    16861 aaaaaatatc ttttacaatt cccatgacga ttcctctttt catataatca aattttcaat
    16921 tgccatactt tgttgctcca aaatcttttt atgtcggtct gataagcgtg cattctgtac
    16981 catatcctgg atataggttg tccgattcaa tcgatccaac tccttagcca ttttccacgt
    17041 aggggcgact tgtcggccta gccaattcaa ggttcgttca atggtatacg gttttggctc
    17101 ggaagttaat tgaatctctt tccgattccg tccaataaaa cgcccccatt gttcattcaa
    17161 gggccagttg gttcgccttt taccttctac tttatccgca aatcgcaagt aacgattgat
    17221 aatggaaaac gtggtgctct caatgctacg accttttaac aaatcttgaa tcgcatgaaa
    17281 agcacggtca tttttcagtc gaatctcaaa acgatttttc gtctcagttt ggtctaatgg
    17341 aatccctaac ttgatatatt gttcatagtc tttttcatac aaacagaaat aaacctcact
    17401 cttgagactg ccgatatata aggtattgcc catgccgtcc ttttcatcag cctttaaaag
    17461 ttccccggaa cgataacttt taaaggtacg aaacaaggag atacattctt ccttctgaca
    17521 ttttttcgtt agctcaggta tatccaatag ccccactcga tcattgattg ccaagtccaa
    17581 gcgtttcatc acgccaccgg cctttagaca atcctcgaaa aagtcgtacc agctacgctt
    17641 ttgagcgagt aaaaaagttt caaattgtcg gcaacctcgc ccctttaatt ctaaaagcac
    17701 acctttatct tcttcatgag acaacatcac cacaatatca cccatgacat actgttcttg
    17761 ataggagtaa aaggcataat cttcatggag catatactca aatttcaaat ggagaatttc
    17821 ttgaataatt tttcgtgcat ccgttgtggg aaagcgaatc cttacatagt caaacagtaa
    17881 cgtcaaaggc aaatcgggat taaagctttc cagttggtca aaaattttcc tcatggtttt
    17941 tatagatgcg gttacgccac cattttctat ctgatttaag tacggccgac taagtccaca
    18001 agccgtagcc aatttatttt gtgaaacgcc gtattcgttg cgtcggtctt ttaatagccg
    18061 ataatctaaa ttccgttgtg ccaagttctc acctccaaat tcaaggtgtt aactttttca
    18121 aatgagttaa cacctataca aattacttac tatctttgat acaacaagca ttcttaatgg
    18181 ttgcttactt attttttgtg tttttgtacc cccctgttag atacgggggt gagacatcat
    18241 ggtcggctaa cgccgtccat gaacaggcac agcaccacgg aggatttctg cttcgcgaat
    18301 cctccgcggt cagcgcctaa cagtcagttg tgttggttta ggttactcat ctgtaatacc
    18361 atttacttcc aactctgctt tcatttctaa caagtattga cgaatatctg gttcttttgc
    18421 ttggtccgca atccgctcaa attctgcaat tacttgattt atttcaattc gagcatccgt
    18481 ctcctcattc attaaatatg gatcgccagc gactttgaca ccattcttgc gaatatcaaa
    18541 ttttaaccgt cgcaattgtt cttctaattc tgggtctgac tgaatctttc caatcaccca
    18601 ataaatttct ccaatcaact catcataaac catgtacatc caactctttt aatcttctgg
    18661 caatatcaaa aatgaccgga actgttacag agtttccggc ttgcttatac aactgactat
    18721 ctgaattgac ttgagaatct cgatcaaaag cccaatcggg aaagccctga agtcgccaac
    18781 actctctagg tgttagcttc cgaatttgat actgatccgt gacgatcgct tgttggtctc
    18841 ctgttagtaa ggtatgaaca ctctgttttc ccagacgacc tcggcgactt gtggaattag
    18901 gaaaagccag attcacactg tcttctggct tcacctcttg atattctgtt tcattttttc
    18961 gaatcatgat ttttgtacca ccttgtttcg ttgacaaggt gggggcggtt ccagtaactt
    19021 cataaactcg attaccttgt tcaaactttc ccggtaatag tcctgctaca gagattccat
    19081 cccttgctgg cagtgcgatt ttgattcctt catcacctgt taaggttggc gctacgccat
    19141 ctgaaccata gacttgtcca cccattcctc gattggaagg attgatattt accaaatttc
    19201 tgatgttttg atcagatgtt gaagacggtc caatgatagg aaatacttgt tcggtacatg
    19261 cgtctcgaga atgtccgata aggaatatcc gctcccggct ttggggaatg taggcctttg
    19321 agttaagcac ctaccactcc acgtcatacc ccaatccatc cagcgttcgg aggatcgtct
    19381 cgaacgtagc ccctccttcg tgattgagca atcctcggac gttctcaagg aataaaagct
    19441 taggtcggag aagagtggtg aaccgagcga tttcaaagaa taaagtacct cgagtatcgg
    19501 aaaatctttg ccgctttccc gcaattgaaa aagcttggca cggaaatccg ccgcaaagga
    19561 tgtccactgg tccgagggat tgaataaact catctgatac acttgtgatg tcatgcattt
    19621 ccacctcctt acttgtatca tgaattgctt tataactacg tctagcaaat ttatcaattt
    19681 cacaaaaacc gatacaatga tggctcgcct gttccatacc taaacgaaag ccaccaatac
    19741 cggcgaataa atctaaaaat ctcatacatt acctcattca ttgcaaagaa aaaagccaag
    19801 aacattttgc tcttggctca tacattatta atttgtccta ctatttttta tttaatccaa
    19861 ctaatttccg ttgtttttca ttggcctgat cggctacata tttaatcacc gttttgaact
    19921 cttttgcagt agcattttcc gataaatccg caatgctctt attgtatttc tttacaattt
    19981 tcgttacttg ttctttctca gtcatgtaat tgaacctcca aatctttcct actataccct
    20041 agtggtaaaa ataattctag attatccgta gcataatgaa cttcggaaat attattcgta
    20101 taatctttta ttatgatatg aagcaataca tctggatgtt tactctttac ttttgcaatg
    20161 ttctctcttg atttgaaaaa agcgttaata aatgcctttt tcggcacatg acgtccctct
    20221 gcaatttcac gttcttgtgt aaatttccaa gcgattttag ggtcttgata aacatagaaa
    20281 atcgtcactc gaaaatgttt tttcaacgcc cgcgagatat ttttttcagc acttaatatt
    20341 gcaaaagttg catccagaat aaaagaatag ccatgatcta agacatatct aaatgcctgc
    20401 tctaccaacc aagaagatgc tttttgaaag agactagagt tttttccatt ataatcagga
    20461 aactgggtgc gaaagtaatc cgcatcaata atcacatggt tatcataatt ctcagctaat
    20521 ccaatggcaa cctctgattt tccagcccca ggactccctg ccataaaaac tgcgtctcgt
    20581 ccaacttcgg gggatttccc tttgataata taagaaataa acttatcctt atttgctttt
    20641 gcatactcta aataagaatc attactttcc atattaatta tcccctttct tcctactata
    20701 taaaagtatt atacgttact tgaagtataa agtcagtatt gaagatcact caatcattca
    20761 tcttttctaa tttactaatt gaatctaaga aatcataccc ttttggtaca agtggcgtat
    20821 aaaactcact aatcacactg ccacctgtat ccacataacc tcgacctttg atgtgcttca
    20881 taaagaagtt tttttcgacc tccccaaaca tcatggaata tccgagttca ctcatacgac
    20941 ccaaagccac gcgaaagttg aattgatcgc ggattccgtc tcctaaatat ttcgcatccg
    21001 gcctttggca agccaaaatc agaaagaaac cagattgacg acccaacatc acgatttgtt
    21061 tgagcttatt caaaatcacc gcactttctt tcgtcgttaa catctccata taagccacgt
    21121 attcatcaaa gattaaaaag ttgggtggaa gtccaagata ggcataattc tctcctgttt
    21181 tgtagttaga catttctttc attgccttgc tacgagccat catacgttca tagaaatctt
    21241 ccacacaagc agaaatttct tccttttgtg aataaacatg aggcatcacc gtacctaaat
    21301 cagctaaatc tgcatttttg ggatcgagga taaacaattc tgcatctgac ttcaatagtg
    21361 cttcgataat ggtgaggagg aaataggtct tccctccacc cgtgccacca gcgattaaca
    21421 tatggggtaa agaatcataa gcccatactt gatttttcat caatcgcaga gtcccatttt
    21481 ctgccactac ttcgtctatc ccaattcgat tggcgatcat atcatacagt aaagtatact
    21541 ccacatagga gtctttgagt tccttttcta ctaactcaca atacagtccg ctttctaact
    21601 tcttttccaa ttttaaaagc tgatcttggt atttcccaag tgaaatttga acgcgaatag
    21661 acagtaatcc atctttcatt cgataacaga ttttggggaa gtaactgata gtctctctgg
    21721 ttcggctact attcaaatct ttgaaaaagc cttcactttg agtttgtttc acttcatacc
    21781 aatggttctc tagaatcatc cgagctagct tttgtcgatg ccacaagctt cgccaatgat
    21841 caagaaaaaa gtgaatataa agcaatatca tagctcctga aaaagcgaga cttccaagca
    21901 gacatactaa atcttttata gaccaaacga tttcaatagt agaaagtgat agcttagaaa
    21961 tatcaaattg tctaaactct tgaaaataaa agacgctcat ccaaccgatg aacagagcga
    22021 gtagccagcc aagaacaaag tgataaacca agtgctgatc tcctgcgcgt atgcgatgtc
    22081 ctttgtacat tttgatggtt tccacctcca atcgaaaaag cccaatagga tttcattagg
    22141 caatttcaaa aagttatgct agtcatttac tgctacgagg aggggagttg tttcgtttca
    22201 accaattgat ttaaaaagcc ttcaatagca tccggcatct ccacaccaat ttcttccaaa
    22261 atggattctg cctgccgaca taagttttca tttacttcga caactataaa gaaagactct
    22321 tcttctccta accagtctag tacatcagag tcttcttcac tgtcgctatt ttctccaagt
    22381 aaaccttgat gtataatcag aaacattttt tcatcagagg ttaatgcttt aacgagttgt
    22441 tcctcagtca gctcgccttt tccatattga tcgagacaat ctaaaatatg ttcaacatta
    22501 atttccataa tcttcaccta tttctttctt tcattttggt tcgtaggatt tccttgattc
    22561 ggctgccgct tcaagactaa atcatccgcc ttaatgaacc aattaacggt agttccttct
    22621 cgaaaaacat aattggcaac ggtatccacg attggattga ccaactctac ctctgcatca
    22681 taagcaaact cgcgtaaggg tacattagct gggatactaa cttgaatcat tcgcccttgt
    22741 gcagaggatt ttaaattata ggttcgttct tttacctcat taccaattgt tccatcctca
    22801 ttttgcttcc gtacttctcg gcgtaatgcg gaaaaacgca acttcccaaa tgttaaacct
    22861 gaatctacga caataccttc tgttaatctc atcttctttt cctcctatgc tttcactaaa
    22921 tcatcaacat acaacacgta atcagtaaac ccacgattct caatctcata gcctttggca
    22981 accaaatggg gattcacggc ttttagcggt tggtcttgat caaaatcctt ttcaccggct
    23041 tccgcagcta ctaccacctc aatatcatcg gcccgttgct tactggaata caaatgataa
    23101 ctgcgaccaa tcaccgtagt attccgtcca tatccttctg ttataatttc tccttcaccg
    23161 gcataactta actttccaaa ggtttcagcc atatctggca cgacaaattt caattccatc
    23221 ttcttttcct cttttcattg ttagttttgg atacaaaaat agcgcgacta cttttttagg
    23281 aggcggtcgc gcacgttggt tcttaggatt tacttgtatc atctcatcac cttagttccg
    23341 gcgattccag aaataatagc ccgccgtcgc aaaaatcaaa gtaaaaccaa tgaacagtaa
    23401 aacattggaa tttttctccc cggtttgagg gaaggtcttc actgtgggac tagattcttg
    23461 agagccactt ggaacagttg gtgtttccgg ttgtttcggc gtacttagta tggttggaac
    23521 ttcttttggt gttaaggttt gagatttttc tttcaaatct tcattgtgtt ttccattgat
    23581 attcccatct ttatcgtaat tgatttccgc aaaagtaaac ttggttcctt ctggatattt
    23641 tccggtatct acttttttcg caagcacagt tttggtgaat tctttatcat ttacttcata
    23701 atcaatcttg ccagatttcc aaatttcttt gtttgtacca tccggtaata aagcatacag
    23761 aattgtttcg aaagcttctt ttgagccatc cagtacatca tgagtaatcg acacatcatc
    23821 aaacatatcc accacatcac catgagtaaa ggtctgagaa ccgttttcta ggtgggcctt
    23881 tgtttggata gaaacatggt gttccactgt acaactgaca gtttgggctt ggttattcaa
    23941 gctaacatcc ttcgctactg gcttatcgcc tgcttcaaaa gcttccttat tttcatacac
    24001 atagttgaat aagacaatag ttttatttaa aacttgttcg gcggttaatt tatgctgaat
    24061 tttccaagtt cctgttttct ctttgttcgc caacgttgcg cttgtttctg caatcaccac
    24121 tggtttggct ttttcatctt tttcttgggc agcttttgtc gcatccacat caatggcttg
    24181 cgctaccaca taccagtctt ctttgatttc atgtagattg taactcaatt tatcaactag
    24241 ctcggtagaa tccagagaag ttaattcttt gtttccgtct ttccaagtcg caagagaagt
    24301 taaactatct tcttcctcag gcaaatcata gagcattaaa cggttcaaac taacagaaaa
    24361 tgctttgttc gtcagcttct cgtaaggaac ggtcaccgtt ttaattggtt gtttttggtc
    24421 ttgttcggta atggtaaaga catactcact cttcacaaag tcttctttgt tttccttaaa
    24481 agtagaacgg atttctaatg gcgtaatctt ttgaaaacct tctggggctt ctacttcttc
    24541 aagtaaataa tccccataag gaagattttc aaacttgcca taaccatcaa accctaattg
    24601 ctcgttataa gctgtggtcg cttcatcttc ggcacctgtg atttcattgg tcccttccaa
    24661 tggcgacact ttaaaggtca gatcattgaa gccagtttcg gtagtgcccg cagctgatcc
    24721 agcaaattta aagaaatcaa agccaaagcg aatcacttgt tcttttgccg taacatttcg
    24781 ggtaatcacg gcattttttt cgctatcatc cacctttttg atggatacag gatactttgt
    24841 ttcatcaagg ctatatcctt cgggcgcttt tgtttcttgc caataatact cgttgatagc
    24901 taaatgttta acggcaactt ggttcttttc gtctaaagtc aaagtgaccg tttcatcaga
    24961 acctttcgtt cccttcgcta actctggttt aaaagcctca ctccatttaa cagcttttcc
    25021 atccttagca gtaaacaagg tgtattcagc accctcaaag actgccttac cttgtgcttt
    25081 atccccagta tctttgtctt ctttcgtcaa agtggtttcg ccagtgactt cttgattttg
    25141 cccttttacg ttactggtaa caggggctac ggtttgattg gcatacttca attcgacttt
    25201 tacaggtttg aacgtattca cgaacccatg actagcttta gtttcggtca cataataggt
    25261 tcccaattcc aacgcattag caatctcttt tggtgtttcc gcatgaccgt tttcatccgt
    25321 ggtcatttct tgaacgattt caccggttgg actgtctttg cgaatggcaa acgtattacc
    25381 agctaaggaa taattgtcat tccaaagatc actccctgtt tcaactccgc ttttatccag
    25441 aatgatttgc cctttttccc gtgcgttttt tgaagtcacg taaatggttt cgcctgcttt
    25501 aatggtggta gtcatggggg tggtatcaat cgtataaggc gccggtactg atttttcggt
    25561 aatggttact tttgtaccat gaggaatctc atccaatgta gcaatgcctt ctttgtcagt
    25621 agtcacgtct tttgcaggta aagtttttcc aaagtctaaa tggaaaaccg ttcctggtac
    25681 aacatcaccc gattctttat cgactttttt gatctttaaa gaaccctccg tttccacatc
    25741 aattttgatt gtgtaggtat taggtttatc aatcgccccg gccattaaag tctgttggcc
    25801 agccttttta taggcgacag gtgttccggt accagcagat ttcttaagcg tcagcacacc
    25861 actttctttt gaattggcat ttggggtgat tatcaactga ttaccattca cacgataatc
    25921 aatgtttgcg gcattttcca caacctcatc gaactctgac aaattcagat tattcgtatc
    25981 cgtcacagta gtcgattgcc ctaatactgt tttagccgta ctattatgga aggttggttt
    26041 cttctgataa tcgtcaatgg cttgatttat ttttgcctca atggctgcga tatttacggc
    26101 actaccatct gatcgtttta ttgagtgaag ggtataccca ttaacttctt gccagatcat
    26161 cttctgtgcc acaatatgag tatctgcatc tgtacctact tttttccata aaacggaaac
    26221 taatttggct ttatcggaca tatctggtaa cgggtttttc tcatagccag gagtgaactc
    26281 attgtaaatc gggactcctg gttcaataca gaaaatatct tgtttaaagc cgttgtacac
    26341 tgcataatgc ggaacagtga cttctgacat tggacgacca tcactaaaag ttccttcaac
    26401 agcatattga tgtgctaaat ctagttctac ggttaccgtt tgtggatggg taatttcatc
    26461 agcaaaagta atcgtaggag caattgctcc aacaattgct tgaacagcca aactcaaaat
    26521 agttaataca cataatttct ttttttgttt tttatacatg aaatgttctc ctttcaaatt
    26581 aagcacaaaa aaataagatg ctcgcttgaa catctttgtt tataaagtct ttttaaaatt
    26641 tttattgact gaatagaatg gatttgagaa tgaatgagcc taaaaattta atctaaaata
    26701 cgaaatatac tgcctttaaa ttagaacaca ttttagaaca caaacatttc aaatttaatc
    26761 aaatataata aaatatattt acgggaaaac ctttataaat caaggttttt caaacttatt
    26821 caatgatacg aagaaaaaac caggtcttaa gaaagcccgt aaagcttcac agttctcaaa
    26881 acgttaattg cgattatcgt tcgagttcac gagttacgag aatcgtactg ggcgaagcaa
    26941 tgctgagcgc agttacctta atcctaactt ttctggcact ttccacaatt tggaaagtgc
    27001 cttttttgtg tttttagcta tgttaatagg taggattctt ggtctataat atcattcgat
    27061 ctataaacaa acgggaaaaa gataaagtat catcaaacaa tattatttat ccgcttctga
    27121 ggttgtgaca gaagtggtca gcttcaagaa ataagaagga atttacaaaa attgcttctc
    27181 atgtttgata aatattgtga cttgtgaccg aggagttggc aggagaacac cgtttattcg
    27241 gaagaaggga ttgtgaaaag tcttttgtca taacctctta tttggtagct aattgtaaat
    27301 cccaaacggc atgtattatt tttcataagt ccgtttttaa ggattaatgt tttcttttgc
    27361 tttttatatg gtctggcttc agcgagaagt tccaacgcat atacaaggac gacttatgtc
    27421 aacgataaca acgattacca tggtggccag tccacttgga ttagcactgt ttggtgcgct
    27481 ctttgattgg aagacaacgc aaccacgtct gcgagacagt ctaatttttc tgggagggag
    27541 tcttgctata gttatcctta taaccggtct ggtaaaatta ctcaagcttg atctgaaaga
    27601 agcaaaaatt ttcagtgaag aggaatacag agaaacgact ttggtcgaaa agaaagaatc
    27661 aggaaactga tacaagcgta taacaaactg cctgataata ggaaagacac gtattgtccg
    27721 attgtttttt ttgacattca attggaaaat ttacactttc tttatacatt tctggctgct
    27781 ttctttatac ccgtcaacta tacttcactc atcaataaga aagaaggata agtatggatt
    27841 ttggaaatga aacggtcttg aaagcaactg gtataaccaa aaaatacggt gctgccaaag
    27901 cattagataa agtatcaatc gaaatcaaac gtggaatgat ctatggattg attggggaaa
    27961 atggagcggg taagtctacc tttatgagaa ctattatggg tttgatctcg attgatgaag
    28021 gttcgatcga attatttgga acgacggatt tgcaggcagc aagacgacgt atgggtcaat
    28081 cgattgaaac ccctgctctt tatccagaat taactgcaag agataaccta cgtatccaag
    28141 cagcgaacgg cggtgtgtcg gatagagaaa tcgaagacct gctgaaaatg atgcgattgg
    28201 aaaatacagg gaaaaagaaa gcaaagaatt tctctttagg tatgcgccag cgtttagcta
    28261 ttgcaaatgc actgatcacg aatccagaat ttttgatttt ggatgaaccg acgaatggga
    28321 tggatccagc agggatggca gaaatgcgag aaatcatcca acgtttggta aaagaacggg
    28381 gcatcaccgt cttgctttct agccacttgc tagatgaatt gtcacagatc gccactcact
    28441 atggtatttt gcatgaagga cacttgatca aagaattatc gaaagaagaa ttggcacaag
    28501 aatctcgtca atttataaaa attgacactt cggcgacgga acaagcagta acggttctcg
    28561 actctcttgg ttatagggat tattttgtac aatctagccg tgttatccag ctttttgaag
    28621 gaattgacca agtagcagcc atcaatcaag cattggtaga agcaaaagtt cctgtggatg
    28681 gtattcacct agttgggcaa aaactggaag attatttctt acaattaact ggaggcaatc
    28741 cacatgttta atctattaac tgctgaaaaa ataaaagtat ggaagagccg taaaatgtgg
    28801 gtctcactag gactgatggt cgtacttccg cttttttatt cttggaacga atggtatatg
    28861 catacaaaat acggcaatga attaaaccaa gcaacggata cagtcatcaa cggtgcaaca
    28921 gggattttaa tggtagaaaa aatggctgca tggattcttc tggctttcgc agcatttgcg
    28981 tgcttttata ttggggaaga atttcaaaac ggtacgattc gcaatacgtt atctttagga
    29041 cggaatcgaa tgacttatta tgtgtcgaaa cttctcgtta cgcttttgat cacaacactt
    29101 ggcgtggttt tgatcacggg actagccatg cttgcttaca cacttgcttt cggatttggt
    29161 gaagttgagg gaatcaaaaa ctatggaaat tatgttctaa aagtatttcc tgtattgctt
    29221 ctgctgacat tagggacatt atctgttcca gtggctttga cattcatcac tagaagtacc
    29281 agtgtcagtt tgcttctttc ttttctttat atcatgggaa cggccttcat tcccggtgtt
    29341 ttcgcaaaaa tcaaaggctt ggaatttcta acggagtggt ttacggaaac gtggctcatg
    29401 tatacggact ttgcgcaaca agcaacttat tcgcaagcac cgaaaatgat tctggtcagt
    29461 ctagtaacaa tcgtggtatc atcagctcta ggaatgttta ttttccaaaa atctgacatt
    29521 aagtaggtgg tcctgctgga aactattttg attatcgaag acgatgaagc tgttcattcc
    29581 ttactggagg aggttcttga acagagatac aaacttcttg atgcttattc aggtacggaa
    29641 ggaaaattat tgttaagcac atatcctgtc gatttgatcc ttctagatct catgcttcca
    29701 gggctttcag gtgaagcatt gttagcagaa atccgacaga catcgaatgt tccgatcatc
    29761 gttctttcag caaaaagcga tcaaagagat aaagtttcct tgttggcagc aggagcggat
    29821 gactatgtga cgaaaccatt tgatatcgaa gaacttttgt tacgaatcgg catccaactt
    29881 cgtcatcaga cttctgtcca agtaaaagat ctgtcgcaac gcatctatta caaggaaatc
    29941 ttactggaca aagagtcgag ggatgtaaat gtgaatggtg tggctgtcca tttgacagga
    30001 agagaattcg atctgctgaa gcttttttta gaacatccga aaaaagtatt ttcgcgagca
    30061 aatctttatg aaacagtttg gcaagaacca tattttgata gtgagaaaac agtgaatatg
    30121 catatttcca atcttcgagg gaagctagca aaggcacatg ctgtgtatat tcatacggtg
    30181 tggggagtgg ggtttagatt tgattaaatg gctgttgctt ctcgttgtta tctttatggg
    30241 aagttttatt ttcttcctgc aaagggaaat caagagatta actaagaaaa tcgccactct
    30301 ttctgaacga caagaatatg gtggtcgtct ttcagtcgaa tttcgagaaa agaatctaat
    30361 ggacctagta gatgcactga atacatggtt ggatgaaaat gaacaaaaag gacaagcatt
    30421 tcaagaaatg gaagaaaaca tcagactatc gatcgtcggc ctttctcatg atctcagaac
    30481 tcctttgact gctattaacg gttatgtaca attactggaa caggtagagg atacggagaa
    30541 acgggagaag tatttaacga tcatcaaaca atctgttgca agattactca gtatgacgga
    30601 tcagttttat gatcttgctc gaatagagac gaagcaaaaa gaaatggatc tccatacgct
    30661 ttctttttct aaagaggtgg aagaagtatt tttctctttt tatgaacaat tttctgagaa
    30721 aaatatcgag gtagcgttcg ctgaacactc gataaataca actgtattag cagatccgct
    30781 catgttgctg cgagtgctgc aaaatatcat acagaattta ttgcgttatg cagatggaca
    30841 agcaaaggtg acattttcaa aagaggatcc attcattcga ttaacagtgg ccaatcctat
    30901 caaaccagga acgcaaacct ctgttgaaaa agtcttccag cgtttttata ccgaaaatac
    30961 cagccgcaca aatacggaat ctagcggaat cgggctttac ctgtcaaaaa aattggtgga
    31021 aggaatggga ggagagatga ccgctagatt agacgggggc atcttctcca tctccgtcaa
    31081 gttgcggagg gtttaattat acaagattag tgagaactag taga
//
LOCUS       DAZKOK010000016        30470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000016 DAZKOK010000000
VERSION     DAZKOK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.016"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..774)
                     /gene="pyrE"
                     /locus_tag="ACG90W_000740"
     CDS             complement(145..774)
                     /gene="pyrE"
                     /locus_tag="ACG90W_000740"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0477125.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(781..1497)
                     /gene="pyrF"
                     /locus_tag="ACG90W_000741"
     CDS             complement(781..1497)
                     /gene="pyrF"
                     /locus_tag="ACG90W_000741"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0477126.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1494..2426)
                     /locus_tag="ACG90W_000742"
     CDS             complement(1494..2426)
                     /locus_tag="ACG90W_000742"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0477127.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2423..3199)
                     /locus_tag="ACG90W_000743"
     CDS             complement(2423..3199)
                     /locus_tag="ACG90W_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0477128.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3253..6438)
                     /gene="carB"
                     /locus_tag="ACG90W_000744"
     CDS             complement(3253..6438)
                     /gene="carB"
                     /locus_tag="ACG90W_000744"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0477129.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6443..7519)
                     /locus_tag="ACG90W_000745"
     CDS             complement(6443..7519)
                     /locus_tag="ACG90W_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0477130.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7524..8804)
                     /locus_tag="ACG90W_000746"
     CDS             complement(7524..8804)
                     /locus_tag="ACG90W_000746"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0477131.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8823..9749)
                     /locus_tag="ACG90W_000747"
     CDS             complement(8823..9749)
                     /locus_tag="ACG90W_000747"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0477132.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9762..11045)
                     /locus_tag="ACG90W_000748"
     CDS             complement(9762..11045)
                     /locus_tag="ACG90W_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0477133.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11059..11598)
                     /gene="pyrR"
                     /locus_tag="ACG90W_000749"
     CDS             complement(11059..11598)
                     /gene="pyrR"
                     /locus_tag="ACG90W_000749"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0477134.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11833..12744)
                     /locus_tag="ACG90W_000750"
     CDS             complement(11833..12744)
                     /locus_tag="ACG90W_000750"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0477135.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12741..13205)
                     /gene="lspA"
                     /locus_tag="ACG90W_000751"
     CDS             complement(12741..13205)
                     /gene="lspA"
                     /locus_tag="ACG90W_000751"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0477136.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13210..13908)
                     /locus_tag="ACG90W_000752"
     CDS             complement(13210..13908)
                     /locus_tag="ACG90W_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0477137.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13895..14281)
                     /locus_tag="ACG90W_000753"
     CDS             complement(13895..14281)
                     /locus_tag="ACG90W_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477138.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14428..16098)
                     /locus_tag="ACG90W_000754"
     CDS             complement(14428..16098)
                     /locus_tag="ACG90W_000754"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0477139.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16281..16514
                     /locus_tag="ACG90W_000755"
     CDS             16281..16514
                     /locus_tag="ACG90W_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0477140.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16613..17044)
                     /locus_tag="ACG90W_000756"
     CDS             complement(16613..17044)
                     /locus_tag="ACG90W_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0477141.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17123..17536
                     /locus_tag="ACG90W_000757"
     CDS             17123..17536
                     /locus_tag="ACG90W_000757"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0477142.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17574..18722)
                     /locus_tag="ACG90W_000758"
     CDS             complement(17574..18722)
                     /locus_tag="ACG90W_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0477143.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18844..19440)
                     /locus_tag="ACG90W_000759"
     CDS             complement(18844..19440)
                     /locus_tag="ACG90W_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0477144.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19640..22282)
                     /gene="alaS"
                     /locus_tag="ACG90W_000760"
     CDS             complement(19640..22282)
                     /gene="alaS"
                     /locus_tag="ACG90W_000760"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0477145.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22670..23101
                     /locus_tag="ACG90W_000761"
     CDS             22670..23101
                     /locus_tag="ACG90W_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477146.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23146..24495)
                     /locus_tag="ACG90W_000762"
     CDS             complement(23146..24495)
                     /locus_tag="ACG90W_000762"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0477147.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24565..25728)
                     /locus_tag="ACG90W_000763"
     CDS             complement(24565..25728)
                     /locus_tag="ACG90W_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0477148.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25857..26180
                     /locus_tag="ACG90W_000764"
     CDS             25857..26180
                     /locus_tag="ACG90W_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477149.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26363..27712)
                     /locus_tag="ACG90W_000765"
     CDS             complement(26363..27712)
                     /locus_tag="ACG90W_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0477150.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27781..28734)
                     /locus_tag="ACG90W_000766"
     CDS             complement(27781..28734)
                     /locus_tag="ACG90W_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0477151.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28836..30026)
                     /locus_tag="ACG90W_000767"
     CDS             complement(28836..30026)
                     /locus_tag="ACG90W_000767"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0477152.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30226..>30470)
                     /locus_tag="ACG90W_000768"
     CDS             complement(30226..>30470)
                     /locus_tag="ACG90W_000768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477153.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         8561 a         6686 c         5348 g         9875 t
ORIGIN      
        1 aaaaaatcaa aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc
       61 tcgcaatcag tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga
      121 tttctctatc ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc
      181 ttttagcaga gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa
      241 tacagaataa ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc
      301 ttttggcaac tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt
      361 tgctgcttca gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac
      421 catcttctga ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc
      481 cttacttcga atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg
      541 gattcctgct gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt
      601 gcttgctaat ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt
      661 atcacagtaa attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa
      721 aaatactgct tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg
      781 ttagctccat tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat
      841 tggtcgacct ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac
      901 tcttgtctga tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa
      961 ttcgtctcct gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc
     1021 taatcctgct tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc
     1081 gattagttga tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag
     1141 aagtagcgga cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc
     1201 catcatacga ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa
     1261 tccccgcatt gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac
     1321 attatgacca gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc
     1381 cattccgatt ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac
     1441 ctcttgccta gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt
     1501 tcacgttcct ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc
     1561 attcgcacag ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg
     1621 gtccctatag ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc
     1681 tgcacgccgc ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa
     1741 cgaatagcga caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc
     1801 ggttttcttg tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca
     1861 tctgcccctc cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac
     1921 aatttgacat agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca
     1981 acatcagaat ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc
     2041 agctcgattg ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct
     2101 tcttcacaag caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct
     2161 ggaagaagtt tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac
     2221 atgccagatg gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc
     2281 gttgccttga ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg
     2341 ccaaaaccga aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt
     2401 ttgaccgcta aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt
     2461 cgtcacaaac tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag
     2521 catagcaagc ccctatccca catgccatgc gggattctaa tgataactgg acattttgat
     2581 gcgaagaaaa cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag
     2641 ctgtaggttc cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg
     2701 ttccatcgtc agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa
     2761 cgacttcatg tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact
     2821 gttttgataa ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt
     2881 ctcctgtatg aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat
     2941 ggtctccggc agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga
     3001 tacggcaagt atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca
     3061 agtctgcacg cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta
     3121 gatcaccttt caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt
     3181 ccatcatttc ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac
     3241 atgtgctcgc cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat
     3301 cgcatctgct gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg
     3361 gatgatgaaa ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac
     3421 ttgtgtttta ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat
     3481 tttcgctact gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt
     3541 tcctatcaga ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt
     3601 tgtttcgtcc gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc
     3661 ttcgaatgcc ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt
     3721 cgatttcatt tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac
     3781 aggtgcttta acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt
     3841 ctttagtttc tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac
     3901 cttacttaag aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc
     3961 attttcatgg atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa
     4021 gcggatcgtg taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata
     4081 cacagccatt gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg
     4141 gatgagtact gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta
     4201 acggtccact agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc
     4261 taaatctcgc tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg
     4321 aacaagaact ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc
     4381 agtatcccct aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg
     4441 attttccgca cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt
     4501 caaaggttcg gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg
     4561 ctgttctagc tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt
     4621 atccgaaata gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc
     4681 gtatcctgcc tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc
     4741 ttgtccgata cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga
     4801 actttcattt tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc
     4861 tgcacaagtg tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg
     4921 aacggcttct tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg
     4981 tttcgctgtt ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc
     5041 tacgatatgc aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc
     5101 ttctatggtg taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc
     5161 tcgaaccact ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat
     5221 atggtgtgta ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg
     5281 accgatagcc atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt
     5341 ttcaaactta tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc
     5401 aaattctgca aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag
     5461 cgctatttta gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga
     5521 tgaacgagaa acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag
     5581 agcgagttgg acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc
     5641 atcacgaagc atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga
     5701 atcacctgta tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt
     5761 atcagccgaa tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc
     5821 aatcaaacat tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc
     5881 ttcttcattt tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat
     5941 gacaggatag ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc
     6001 agactcgggg attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc
     6061 tgcttgatcg atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat
     6121 acctgatgct gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag
     6181 gagagcatct ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc
     6241 gatatatact tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac
     6301 taaaaccact tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc
     6361 aaattctgcg gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat
     6421 atctgtacgt ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt
     6481 catcaaataa atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac
     6541 tgaaagcggg ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat
     6601 gagttacgat gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt
     6661 gagaagtaaa atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat
     6721 gtccgaattt cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga
     6781 gacaaatacc aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg
     6841 gcacatcttt tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta
     6901 aaatcgtctc ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca
     6961 attctcgtaa aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc
     7021 ctggacttgg ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg
     7081 ttgctttcag ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt
     7141 tcatcgttcc gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc
     7201 caggaattcc tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag
     7261 ctacacgtgc atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat
     7321 catctctatt taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt
     7381 taaaactttg atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt
     7441 ctccgaagac actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta
     7501 atataagcaa acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca
     7561 aaagtcattt gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt
     7621 gataaaaagt cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca
     7681 gcgggggcac caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact
     7741 gccaaccaat tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag
     7801 atcagttgaa acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg
     7861 cttttttctt ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca
     7921 agcaagcctt cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc
     7981 caaaagccga aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact
     8041 tctgctgtta cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta
     8101 gtagaaacat ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta
     8161 gcaatttgag aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc
     8221 ttgccagcgt gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg
     8281 gctttattgt tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca
     8341 cctacaccgt catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact
     8401 aatttctcac ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca
     8461 tcttttctaa tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt
     8521 aaattcggca tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct
     8581 tcgatcgtct ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa
     8641 ccaggtgtga tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat
     8701 cctgtaccga ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata
     8761 ttttttcgtg tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat
     8821 aattacgctt ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg
     8881 ctcatttgct gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca
     8941 tcacggttaa ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc
     9001 tcaactgtta acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca
     9061 tggcgttcgt gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc
     9121 aatggcatat aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag
     9181 aagaatactt gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag
     9241 tgagtgatat cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa
     9301 atggtcatca aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg
     9361 ataatcgaac actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca
     9421 tgacggataa ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt
     9481 gtttcccctt tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt
     9541 tttttctccg ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt
     9601 gtagcaaagt actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct
     9661 ctgcggatca agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt
     9721 aagctgatac gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt
     9781 gaagtttctg gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc
     9841 gacaatgtaa atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact
     9901 gaagcaatca acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc
     9961 attccacttg cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca
    10021 gggatactta aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact
    10081 gcagctccac cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt
    10141 tcaccgtaac ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca
    10201 tcacctgtca acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta
    10261 ttcaatacca taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt
    10321 gttgtgattg ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta
    10381 ggaacagcaa accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag
    10441 gctacgatgt atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt
    10501 agccacatgt tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg
    10561 aaatcatacg tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac
    10621 ccgataacca tgatgactgg accgacgacg attggaggca agattttatc cagccatgct
    10681 gaaccgatct ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct
    10741 ccttgtgcaa tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa
    10801 gcgaagctgc ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat
    10861 gtacctagtc ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact
    10921 aacacggttg ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct
    10981 ggggttgggc gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc
    11041 gacatgtttc tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc
    11101 aagctcttgc atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac
    11161 ataatctgca cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat
    11221 ttttcttggt ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt
    11281 atacaaaaca tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga
    11341 agaatgaagt tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc
    11401 cagttctcct acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga
    11461 tgcaatgtag attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt
    11521 tctctcgata atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac
    11581 tacttctttt gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct
    11641 aagaggacag gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg
    11701 tcaatgagca atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct
    11761 cacgggacta aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata
    11821 ccgttatcta aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac
    11881 ttctggaaga ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa
    11941 catttgcgca tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg
    12001 atctccagct aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc
    12061 ggtttctagt tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg
    12121 tgtgacagcc gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg
    12181 gccgattggc gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac
    12241 atatttgcgc aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc
    12301 atttttggca accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg
    12361 acgaataacg ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat
    12421 tgtgccatgc gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac
    12481 cgctacatct tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa
    12541 tgtttctggt tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc
    12601 tttgactggc tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga
    12661 acgactgtaa tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg
    12721 aatcgtcgtt ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa
    12781 attaacatac aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag
    12841 ctcataaaat ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa
    12901 ttgccaatcg caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt
    12961 tcacgatttt taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta
    13021 aagaaccaca tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc
    13081 agtgacaaaa tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc
    13141 caccatttga ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt
    13201 aacaaaaaat caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag
    13261 cagattacca tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc
    13321 acgaaacatc gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt
    13381 gtactttccg ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc
    13441 tttttggctt tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa
    13501 ttttccttta ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag
    13561 ttcaagtaac ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac
    13621 ggtttcaggc tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt
    13681 tggctctgca atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg
    13741 agcaagctgt aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag
    13801 gcgtctgaca agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca
    13861 tttttcgatt cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct
    13921 tgatcaaata tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa
    13981 aaaaactgaa aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa
    14041 acggatagat ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt
    14101 tcactgtata aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt
    14161 ccagatcaag cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc
    14221 ttagctgaat cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca
    14281 tcataccctc ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc
    14341 tagtgtaaac aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc
    14401 tccttcgatg gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt
    14461 cacgtccata ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc
    14521 agtcaaggct actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt
    14581 gaaatcttcc ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc
    14641 catacaaatc ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc
    14701 ttttttgctg aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt
    14761 ttcttgtatc gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat
    14821 caatcgtact acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact
    14881 cgctctcttg gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt
    14941 gtctgtatct gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat
    15001 attacgtata tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt
    15061 tttaagttca tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat
    15121 caccacagca tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc
    15181 gccaagatct gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt
    15241 cttggtagct aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac
    15301 aaaagctggt gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag
    15361 taatgccaga gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt
    15421 atagccgaca acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca
    15481 caagatagcc atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc
    15541 ttggattggc ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac
    15601 tacccgtttc caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg
    15661 attatctaaa agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt
    15721 aaaatgcaag ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc
    15781 acctttgatt cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt
    15841 attgatttta ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc
    15901 tggtgttgga ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg
    15961 attgattcca gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc
    16021 cttaagatct aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat
    16081 ttgcagatca ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt
    16141 ctttgttaag atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag
    16201 gtttttaatt tatttgacgt acttataaat actctttata atgaaggcta tgatcagact
    16261 ttagaaaggt cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag
    16321 gatacggctt tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg
    16381 aagaagaggg attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag
    16441 ggaatcgagg aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta
    16501 gtatgcatga atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc
    16561 actcttttct tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt
    16621 ttcttttttt cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat
    16681 gatcaaaaag gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat
    16741 cttgcaacat catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag
    16801 atatttctcg gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa
    16861 taaaagaacg ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc
    16921 ttttccaata aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca
    16981 aggtcaatga ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt
    17041 tcattctcta acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga
    17101 acaaaaagga gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa
    17161 ccctggtcct agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc
    17221 tatcccgctt tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt
    17281 agaatttcta aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa
    17341 aacactagtt tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct
    17401 tcaaagaatc caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga
    17461 aaacaaaaat aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga
    17521 aaaaaagact ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat
    17581 aaaagataat tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt
    17641 tgactacgta agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact
    17701 acatcaaatt gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct
    17761 gtacctgttt caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta
    17821 tcaacaggca ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct
    17881 ttttctatca gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg
    17941 atgtggactg atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag
    18001 ccaagtatat catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt
    18061 gggcgacttc ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg
    18121 atatataatg ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg
    18181 attgctgctg aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct
    18241 gtagctctca atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt
    18301 gcaaatttca aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca
    18361 ttttctggat ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta
    18421 atgatgatcg aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt
    18481 tcctgatctg ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca
    18541 gtatctgata gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg
    18601 atcattagct tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt
    18661 actccttttt ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc
    18721 aagtttttta ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg
    18781 ttttcctctc ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg
    18841 tcattattct ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat
    18901 gattcccgca cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt
    18961 aaataaaaga gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc
    19021 catgaatcct aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga
    19081 gctgatcgtt attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa
    19141 gccaacaagt actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa
    19201 tggcgttgtt ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat
    19261 caccatgcca tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat
    19321 tgttaaacta atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat
    19381 gatgattgct aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat
    19441 tttttcaccc ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa
    19501 gaccatccga atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc
    19561 aagacttgtt tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt
    19621 tctgtcaaac gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat
    19681 ctggaatacc agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc
    19741 caccgcctac tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat
    19801 ttgattcttt cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa
    19861 caagaatatc tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca
    19921 tatctttcac ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt
    19981 tgaatacatc accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt
    20041 gtagctctct tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag
    20101 gtgatttaac aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt
    20161 acgcttcttt acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag
    20221 aaacgatttt gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt
    20281 cgattgacca gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca
    20341 aagccattgc acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga
    20401 tagcttccca gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga
    20461 tttgaccaaa gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt
    20521 gatttgcatg ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat
    20581 ggtttttgat gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt
    20641 cactcattgg tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa
    20701 catctgtaac aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta
    20761 cttgcccacc catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt
    20821 ctgcgacttc attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt
    20881 cttgttctgt gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt
    20941 gtacccccat tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca
    21001 tttctttttc aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg
    21061 ttaattctac cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag
    21121 tcgtcccgtt ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat
    21181 tgattgtttc attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt
    21241 gttgaagtac ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat
    21301 ataagaatgc ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac
    21361 ggcgtaagac ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg
    21421 aaagggcgcg aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac
    21481 catattttac ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg
    21541 tctcaaagtt cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg
    21601 tatcaatgtt tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg
    21661 aaaatactaa gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag
    21721 gatcgtcttc tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg
    21781 gaccacatgg gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta
    21841 gtggcaggcc aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg
    21901 ttacataaag tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg
    21961 cccagtggat ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa
    22021 acatcgtatg gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt
    22081 tttgggcatt tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca
    22141 atgttgcaac accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg
    22201 cactaggttc aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt
    22261 gtgcactact taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac
    22321 accatcacag ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca
    22381 ttacctctta aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg
    22441 gagcaatttg attagtgact attctttttt cagccaagaa agaattttct gtgagtctta
    22501 aaaaacaaat catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca
    22561 atatctcaat ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa
    22621 gaataaagga atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat
    22681 agttggagcg gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt
    22741 attagaacaa gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac
    22801 ctattatcta tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac
    22861 acctacttgc ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt
    22921 aggcggtcag ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag
    22981 ttatgtagta gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg
    23041 cacagaacct tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta
    23101 aaaacaagag attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt
    23161 tttcttattt ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt
    23221 atttttttct gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt
    23281 tttcttcttc acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt
    23341 ggcacgtcta ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc
    23401 aaatgagacg cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa
    23461 cgtaatggcc gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa
    23521 gtctagatct tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc
    23581 tctagcagct aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat
    23641 cactcgtttt ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg
    23701 gtttttcaag taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc
    23761 taaataagga tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt
    23821 tgttgaaatc agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc
    23881 aatgattgga ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc
    23941 agaaaaaact aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt
    24001 taaaaaaccc atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg
    24061 aatcttcagc gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac
    24121 ctgtggctgt tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa
    24181 attcgagaca cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat
    24241 ctgtgcagct agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc
    24301 attgattttt tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga
    24361 ttgcccaatg atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt
    24421 gggctgtttg aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat
    24481 atcaaaatta ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa
    24541 cacagacaaa gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat
    24601 cgtgctaaca gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa
    24661 aaatgatagc aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat
    24721 gaatactgca ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa
    24781 ctgccccaac cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa
    24841 tgcttttaaa gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt
    24901 gccaagtgtc catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa
    24961 taaaagccac cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat
    25021 cgccgctacc aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg
    25081 tcgagtcaat gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt
    25141 aggaagcata aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt
    25201 tgtcactaaa tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc
    25261 acttagctgc gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat
    25321 aaaagaaagc cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt
    25381 aaaagctggt tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga
    25441 caagaccaat accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat
    25501 ctttggccct gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa
    25561 atacgcacac aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa
    25621 tgttccatac tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa
    25681 tggaaaaaca atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct
    25741 tgattctttg tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc
    25801 tattatttct catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc
    25861 tgaatatgta ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg
    25921 cttatgtaag aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg
    25981 gtttcatgct agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa
    26041 tgactccaca tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca
    26101 ttttcatttc tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc
    26161 agttacgcaa atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt
    26221 tgctatttca tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac
    26281 atttcttcca cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc
    26341 taataaaatt cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag
    26401 caagtgtcaa tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata
    26461 cgataaaagt ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt
    26521 atggtcgaaa caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg
    26581 tataaaaaat ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca
    26641 tcatcatgcc aatagcgatg actagctcta agatacctgc ggctacagga actttagttt
    26701 tatcatgaag tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat
    26761 aagggagacg cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga
    26821 taccaactgt gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg
    26881 ccgcattgct gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta
    26941 ctaataaata aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt
    27001 tcgccggctt tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga
    27061 acatcgttgc tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt
    27121 ggatcggaaa cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca
    27181 caaacaaagg aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag
    27241 aacctaaaaa attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca
    27301 ttgactggga aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg
    27361 ctacatttgc tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat
    27421 ttccgtaaat ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca
    27481 gttcagctcc tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca
    27541 tatgcaaact ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg
    27601 caccgatcac tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta
    27661 attcattttc tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc
    27721 ccctttcata ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt
    27781 ttataccgtc tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc
    27841 taagtcgtat ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt
    27901 tggccaagat tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc
    27961 tggtatgatt ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat
    28021 attgaaggca tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc
    28081 atcgatcgca tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt
    28141 gtactttcct tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc
    28201 tcctggcaag gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc
    28261 atatttcaca taataataat cacatagtag ttctccagct accttcgtta ccccatacat
    28321 ggttgtagga cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa
    28381 cgcgccgatt gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc
    28441 attgaccagc ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc
    28501 tgacaaaaga gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg
    28561 cattttttcg taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc
    28621 aggcatgcgt atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt
    28681 gatcaactct gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat
    28741 cctccctgat cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt
    28801 ctctaatttt aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct
    28861 attttttgat aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc
    28921 atatttctga tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa
    28981 atcccatgtt cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg
    29041 actggagtaa taggtgtttc agacataccg atgttatagc ctactttttt caattcttct
    29101 ttaaaataat tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa
    29161 gtaatcgaag ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat
    29221 ggccgacttc tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca
    29281 acaacaccta tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca
    29341 aaatgcttga cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg
    29401 taggtgatca gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct
    29461 ccatccatgg agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct
    29521 atcgcttcct ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt
    29581 tttgctccgg ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag
    29641 atagcatctt cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat
    29701 tgaaaggcga ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga
    29761 tggattttca gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg
    29821 gttgcttcaa tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg
    29881 gatgctagat tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct
    29941 agtatgtcga tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa
    30001 tacattgtta gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa
    30061 cataccaaac atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac
    30121 gcttacactg attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt
    30181 tcaaatgact tatatagtat gaacaattac cttttagata atgatctatt ttactctacc
    30241 tgggcagata aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt
    30301 ctgaatattt aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg
    30361 tttatcagca atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat
    30421 tcgaaaaaag gactgtgaca aaagtcttgt cacagtccta ttcttccgaa
//
LOCUS       DAZKOK010000017        29079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000017 DAZKOK010000000
VERSION     DAZKOK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.017"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..808
                     /locus_tag="ACG90W_000769"
     CDS             <1..808
                     /locus_tag="ACG90W_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0477154.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1161..2009)
                     /locus_tag="ACG90W_000770"
                     /pseudo
     CDS             complement(1161..2009)
                     /locus_tag="ACG90W_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2162..3274
                     /locus_tag="ACG90W_000771"
     CDS             2162..3274
                     /locus_tag="ACG90W_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0477155.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="ACG90W_000772"
     CDS             complement(3352..3645)
                     /locus_tag="ACG90W_000772"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0477156.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="ACG90W_000773"
     CDS             3813..4127
                     /locus_tag="ACG90W_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0477157.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="ACG90W_000774"
     CDS             4148..6127
                     /locus_tag="ACG90W_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0477158.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="ACG90W_000775"
     CDS             6140..6466
                     /locus_tag="ACG90W_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0477159.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="ACG90W_000776"
     CDS             6482..7000
                     /locus_tag="ACG90W_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477160.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="ACG90W_000777"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="ACG90W_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0477161.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="ACG90W_000778"
     CDS             complement(8436..9941)
                     /locus_tag="ACG90W_000778"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0477162.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="ACG90W_000779"
     CDS             complement(10033..10623)
                     /locus_tag="ACG90W_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0477163.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="ACG90W_000780"
     CDS             10802..12448
                     /locus_tag="ACG90W_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0477164.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="ACG90W_000781"
     CDS             12497..13216
                     /locus_tag="ACG90W_000781"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0477165.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="ACG90W_000782"
     CDS             13695..14642
                     /locus_tag="ACG90W_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477166.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="ACG90W_000783"
     CDS             14654..15574
                     /locus_tag="ACG90W_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0477167.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="ACG90W_000784"
     CDS             15615..17072
                     /locus_tag="ACG90W_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0477168.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="ACG90W_000785"
     CDS             17417..18031
                     /locus_tag="ACG90W_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0477169.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="ACG90W_000786"
     CDS             18024..19748
                     /locus_tag="ACG90W_000786"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0477170.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="ACG90W_000787"
     CDS             19745..21196
                     /locus_tag="ACG90W_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0477171.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="ACG90W_000788"
     CDS             complement(21669..23822)
                     /locus_tag="ACG90W_000788"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0477172.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="ACG90W_000789"
     CDS             complement(23824..24870)
                     /locus_tag="ACG90W_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0477173.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="ACG90W_000790"
     CDS             25020..26309
                     /locus_tag="ACG90W_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0477174.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="ACG90W_000791"
     CDS             26322..29024
                     /locus_tag="ACG90W_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0477175.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         9454 a         5025 c         5898 g         8702 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tagaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggcttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atacttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttaga
//
LOCUS       DAZKOK010000018        27569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000018 DAZKOK010000000
VERSION     DAZKOK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.018"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..1905
                     /locus_tag="ACG90W_000792"
     CDS             139..1905
                     /locus_tag="ACG90W_000792"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0477176.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2007..3266
                     /locus_tag="ACG90W_000793"
     CDS             2007..3266
                     /locus_tag="ACG90W_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0477177.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3413..4714
                     /locus_tag="ACG90W_000794"
     CDS             3413..4714
                     /locus_tag="ACG90W_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0477178.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4717..5568
                     /locus_tag="ACG90W_000795"
     CDS             4717..5568
                     /locus_tag="ACG90W_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0477179.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5581..6396
                     /locus_tag="ACG90W_000796"
     CDS             5581..6396
                     /locus_tag="ACG90W_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0477180.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6547..7713
                     /locus_tag="ACG90W_000797"
     CDS             6547..7713
                     /locus_tag="ACG90W_000797"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0477181.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            7943..8590
                     /locus_tag="ACG90W_000798"
     CDS             7943..8590
                     /locus_tag="ACG90W_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0477182.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8674..9618
                     /locus_tag="ACG90W_000799"
     CDS             8674..9618
                     /locus_tag="ACG90W_000799"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0477183.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9753..11204
                     /locus_tag="ACG90W_000800"
     CDS             9753..11204
                     /locus_tag="ACG90W_000800"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0477184.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11225..12061
                     /locus_tag="ACG90W_000801"
     CDS             11225..12061
                     /locus_tag="ACG90W_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0477185.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12061..12720
                     /locus_tag="ACG90W_000802"
     CDS             12061..12720
                     /locus_tag="ACG90W_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0477186.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12846..13535
                     /locus_tag="ACG90W_000803"
     CDS             12846..13535
                     /locus_tag="ACG90W_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477187.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13541..14497
                     /locus_tag="ACG90W_000804"
     CDS             13541..14497
                     /locus_tag="ACG90W_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0477188.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14541..15053
                     /locus_tag="ACG90W_000805"
     CDS             14541..15053
                     /locus_tag="ACG90W_000805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477189.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15309..15857)
                     /locus_tag="ACG90W_000806"
     CDS             complement(15309..15857)
                     /locus_tag="ACG90W_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0477190.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(15946..17127)
                     /locus_tag="ACG90W_000807"
     CDS             complement(15946..17127)
                     /locus_tag="ACG90W_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0477191.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17363..18871)
                     /locus_tag="ACG90W_000808"
     CDS             complement(17363..18871)
                     /locus_tag="ACG90W_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0477192.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19030..19221
                     /locus_tag="ACG90W_000809"
     CDS             19030..19221
                     /locus_tag="ACG90W_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477193.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19424..20341
                     /locus_tag="ACG90W_000810"
     CDS             19424..20341
                     /locus_tag="ACG90W_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0477194.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20334..21407
                     /locus_tag="ACG90W_000811"
     CDS             20334..21407
                     /locus_tag="ACG90W_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477195.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21555..22073
                     /locus_tag="ACG90W_000812"
     CDS             21555..22073
                     /locus_tag="ACG90W_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477196.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22054..22446
                     /locus_tag="ACG90W_000813"
     CDS             22054..22446
                     /locus_tag="ACG90W_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0477197.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22522..23457
                     /locus_tag="ACG90W_000814"
     CDS             22522..23457
                     /locus_tag="ACG90W_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0477198.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23590..24369
                     /locus_tag="ACG90W_000815"
     CDS             23590..24369
                     /locus_tag="ACG90W_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0477199.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24362..24679
                     /locus_tag="ACG90W_000816"
     CDS             24362..24679
                     /locus_tag="ACG90W_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0477200.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            24923..26533
                     /locus_tag="ACG90W_000817"
     CDS             24923..26533
                     /locus_tag="ACG90W_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0477201.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26690..27538
                     /locus_tag="ACG90W_000818"
     CDS             26690..27538
                     /locus_tag="ACG90W_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0477202.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8916 a         4882 c         5538 g         8233 t
ORIGIN      
        1 gtctatctat ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata
       61 acatttttga aagcgttgtc ttttgtttct ctatcactta tagtgatgac gtcatattaa
      121 aacacaagga ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat
      181 gcatttctat atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc
      241 aaaaaagtat atgtgctttg tggagatcca tataccataa caactgaaaa atggtatcaa
      301 aaacaacgac caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa
      361 gtgacgagcg aaacaaggcg cttacagtat gcgttccatg ttgttgggga agataacacg
      421 gactgttttt acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca
      481 aatttttatt ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg
      541 gtaaaagaaa cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca
      601 aatgaccccg aacacacttt accttgggga agtaaagatc cagaccgaga agacttcttc
      661 gggggagatt tgcaaggtgt gagggatcat ttggattact tgacggacct tggcgtcaat
      721 ggcatctatt tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat
      781 tattatgagg tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa
      841 gcacataaaa gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat
      901 tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc
      961 cacatccgtt ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa
     1021 aatatcccat atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat
     1081 ccagaagtgc aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt
     1141 gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga
     1201 gaagcagtta cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct
     1261 caagcttggc tgcaagggga tgaattccat gcagtgatga attatgcatt cacagattcg
     1321 attaaagatt attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat
     1381 catcagcaaa tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct
     1441 catgatactg ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg
     1501 atggccttta tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg
     1561 atgactggtg aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa
     1621 gatctagagc tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag
     1681 attatttctg aaggttctac tcagtggcta atagtatatg atcgtgaaga taagctctat
     1741 ttcacacggg aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct
     1801 tgggtagtag aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac
     1861 gggaaagcag agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta
     1921 acaacaagaa agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca
     1981 cgttgaatac acaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag
     2041 ggttaatctc tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag
     2101 cagaagggga agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag
     2161 attatataaa cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag
     2221 tagaaaagga catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg
     2281 gacctgacgt tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc
     2341 tggcggaagt aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga
     2401 cttatgatgg aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata
     2461 acaaagattt agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag
     2521 acagtcgttt tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga
     2581 ctgactttta ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg
     2641 atggcacaaa tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt
     2701 atgcaacaga ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag
     2761 gagacttcgc cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat
     2821 ggcaagcgca aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc
     2881 taaataatgg acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt
     2941 atgctaaaaa caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc
     3001 aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag
     3061 caaaggctaa aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag
     3121 cgatgccaaa catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg
     3181 atgcagcatc aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca
     3241 gtgaagccat agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg
     3301 gaacaggtgc ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg
     3361 ccattatctt aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca
     3421 agataaaaca agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat
     3481 ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct
     3541 attcattggt cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg
     3601 aagtatacca atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat
     3661 cattacaatc attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc
     3721 gaagatgcgt caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat
     3781 aggtgatgaa ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac
     3841 gatcattttc ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca
     3901 tattccacca gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt
     3961 tttaagctca tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt
     4021 atgtgcaaca actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa
     4081 atttattaaa ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc
     4141 ttttattact atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac
     4201 tcaagtgatt cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa
     4261 aacggacccg aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc
     4321 ctatatctat gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc
     4381 agcaaaaatc gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt
     4441 cttcctagtg gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc
     4501 catgatctat ctgtttaaca atggtggtcc cggaagcgta ggcggaggag ctggttctac
     4561 tgatattctt atttcctgga tctataaact tacgactgga acttctccgc agtattctat
     4621 ggcttcagca atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt
     4681 taaaaagaca aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct
     4741 cgattccaaa aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc
     4801 attatcatct atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca
     4861 gcttttagtt tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt
     4921 gaaacacttt acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc
     4981 gtacaagtga ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc
     5041 cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg
     5101 actgcctttt atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca
     5161 atgatctata taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac
     5221 actgtaccaa tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt
     5281 tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct
     5341 tttatgggac cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat
     5401 cagactgttg ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct
     5461 ctttttgctg ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta
     5521 caaaagaact ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc
     5581 atgcaaacat ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg
     5641 ggacgcaaac tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg
     5701 atggtcatgc ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa
     5761 tttctatccg ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa
     5821 ttatctgaaa ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg
     5881 gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag
     5941 aaggaacaat gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat
     6001 tatagcccat ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta
     6061 gaaagagaat tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt
     6121 ggtcttgtcc tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg
     6181 atgacaagaa aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg
     6241 ctgaatgtta tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac
     6301 tttatcttga tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt
     6361 gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca
     6421 atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac
     6481 ttcttagttg attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg
     6541 aaaatcatgt tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct
     6601 gtcgaagtaa attattggga cggtacaacg aaacaatacg gagatacgga cactcctccc
     6661 caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcttct
     6721 ttggcactag gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca
     6781 ttgatcaacg atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa
     6841 tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat
     6901 tatgacttag gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt
     6961 gcttatttca atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat
     7021 atcttagaca aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg
     7081 ggaacattga tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta
     7141 agtgaagaac aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa
     7201 tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca
     7261 agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta
     7321 aaagatttat tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc
     7381 ggtgcgacaa atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt
     7441 actgaattag ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta
     7501 cgtcgtgact accaattgac attagaacat tggacaaaga attttcatca agtccaagat
     7561 caagtcacga aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct
     7621 tgtgcagctt catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct
     7681 ggaaacaatg acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact
     7741 atttaaaaag aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta
     7801 ggattgtctc taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct
     7861 ttaattttta aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt
     7921 tataacaaag gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc
     7981 tttgttttta gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc
     8041 cagtagcgaa cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc
     8101 gtctagccag acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc
     8161 agctcgtgcg atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga
     8221 attagatagt gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga
     8281 atacgaagca agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct
     8341 agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt
     8401 gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac
     8461 agattgggat ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg
     8521 caatcaaagt acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca
     8581 agatgattaa gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact
     8641 agacaaaata acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat
     8701 catatctttg ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa
     8761 ttagatgaac tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat
     8821 gaacgaaaag gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct
     8881 atgggacttc caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa
     8941 gtgcaggaaa accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa
     9001 aacttggaaa tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat
     9061 ttatcttgtc ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat
     9121 gaaacactga aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct
     9181 tattttgatt ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc
     9241 tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca
     9301 gtagtcatca aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact
     9361 gctttggcga atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt
     9421 acaggtggga ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg
     9481 ttacagattg gtacagagct tcataaagaa gggccggaaa ttttttctag aatcataaaa
     9541 gaattaacgc agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa
     9601 ttacgcacca tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata
     9661 aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt
     9721 gggaaaataa cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag
     9781 agcttaaaga aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa
     9841 agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc
     9901 acgagctatt gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa
     9961 aaatgctcgg tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa
    10021 cacaccgagc ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc
    10081 cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct
    10141 atggcccagc aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag
    10201 ctgctgctgc agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt
    10261 ctattagaat acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga
    10321 tgccagttgg tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga
    10381 cagtttctat gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg
    10441 cgccatatca agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga
    10501 tagctttttg tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg
    10561 taaaaaaagc tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc
    10621 cactagatgg tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag
    10681 caatgatccg ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac
    10741 ccatttcttg gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt
    10801 ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc
    10861 tattcctttc accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg
    10921 atgcgataag agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg
    10981 ttgtttcttc catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc
    11041 tgactggaca accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag
    11101 gaatccaatt tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat
    11161 ttgaagcaaa tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag
    11221 aagaatgaag aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc
    11281 ttgcggtaca gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa
    11341 agaattagta gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa
    11401 tgaattagtt ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa
    11461 aatctccttt aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc
    11521 agattttgct gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc
    11581 agagactttc aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat
    11641 ccactcactt gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat
    11701 gcgtttagcg gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca
    11761 gtatttaagc gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca
    11821 gaaaatggcc gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt
    11881 caatgaaaac gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat
    11941 cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata
    12001 ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg
    12061 atgtactttc ccctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa
    12121 ggcttggtat acacattagg gattggcttc gtgtcgtttc ttttaggaaa tttggtaggg
    12181 ttgcttttga cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt
    12241 tatttatcgt tctttagagg cattcccgca ctagtcctgc tgtttctgtt atatttcggg
    12301 ttaccttatc aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt
    12361 gcatttattg gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac
    12421 gctggtttag cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa
    12481 gcttttcgaa tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact
    12541 tcgttagcag caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt
    12601 cggacattcg attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt
    12661 atcgggatcg aaaaagtgca acagtattta gaaaaacgtt ctatccatca gtcaatctga
    12721 aaaatagaaa tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc
    12781 tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag
    12841 aaaacatgaa gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg
    12901 agcaaggcgg cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata
    12961 cgcttagctg cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag
    13021 tgacaaaagc agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt
    13081 ttgttttacc aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg
    13141 ttttagtgta tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga
    13201 ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct
    13261 tcatggcttc tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat
    13321 attttccaaa agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat
    13381 tttctaacga acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg
    13441 agttgcaaga agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc
    13501 agatccgtcg gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag
    13561 aggaaaacaa tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa
    13621 taaaatccaa ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga
    13681 attatttcaa aaaaagcacc atccaatcgc tgtgatccaa aatccccaag taggacaatt
    13741 caaattattc gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc
    13801 tgcacagatc ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct
    13861 tgttttcgat ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt
    13921 tgattcgaaa tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa
    13981 aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac
    14041 agatgacttt tttagtgatg actatctttt tttccacgca gacggatata ttggattttc
    14101 tgatttcacg atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc
    14161 tatccatcta acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga
    14221 cagtaacgac tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa
    14281 aaaatggatc atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt
    14341 ggctctttac cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat
    14401 gcatcatttg gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta
    14461 ttcaaaagtt caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg
    14521 aatagaaaag aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag
    14581 tggtagaaat cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc
    14641 aaaacgggca gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct
    14701 atgtattaat agtggaagat caaattgctg gaacagctgc attagtctca ggcgtggatc
    14761 ccgtttacac aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc
    14821 atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac
    14881 atttgttgac gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag
    14941 ctaatgaacc gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg
    15001 aatttccttt tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa
    15061 atcatctgag caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca
    15121 agaggttgta acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc
    15181 tcaaattttt gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc
    15241 atcgagaggt cgtgacatag cttttgtcac gatctctcgg cttatcctac gagtattatt
    15301 cgatacattt atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca
    15361 tcgtttgaca cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct
    15421 tctacataaa ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg
    15481 cgcgattgca atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa
    15541 tactcatata aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc
    15601 agtttctttt taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac
    15661 tctgcaaact catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa
    15721 tcctcttgat ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt
    15781 tccggctgaa aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat
    15841 acatagcaat aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg
    15901 aaaatatttc caaagctttt aaaagtaact ttgctttttt gtattttata ccagcaagcc
    15961 agtcacaata gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg
    16021 ttttgcgaca agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc
    16081 gatagaagag aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc
    16141 agtaaaatgg aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag
    16201 tttagtcgcc atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa
    16261 ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg
    16321 gaagatagca ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt
    16381 gatcgccaca tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc
    16441 tttgttttct tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat
    16501 tgaagccaga atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga
    16561 atcgatcagt gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac
    16621 actcatggtg aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa
    16681 aacttccgat tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat
    16741 cccgttgatt ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat
    16801 atagcgtaga ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa
    16861 gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc
    16921 aaaagcaaga agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt
    16981 tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt
    17041 caatttgatt gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa
    17101 accaataata cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt
    17161 tacctgtttt gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg
    17221 aaacgcattc attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta
    17281 acgtcttagt aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc
    17341 aacatttata agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg
    17401 cgcaggtaat atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca
    17461 cagtagagtg ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga
    17521 ttggattcgt tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat
    17581 ccaaagagga atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg
    17641 acaataaagg aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg
    17701 ccgctgacgc atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt
    17761 cgattgcaat cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat
    17821 agtcatgggc agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa
    17881 tcagtttgat tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg
    17941 tttggaaata acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca
    18001 cgatcccaaa tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt
    18061 taccagtaat tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat
    18121 agttcacagc tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt
    18181 ttagatcgac tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc
    18241 atactgggaa agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat
    18301 acagttggtt caagcaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa
    18361 cgattggttt agttgtttca atatactcat tttttccaaa accatcttct gagacgattt
    18421 tttctacgtt gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc
    18481 tttctagttt attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga
    18541 cactgttgat taataagcca taaccacgta attcatcgat taaccgcaag atatccatat
    18601 cataagtaat cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc
    18661 aaatcagtat ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt
    18721 caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac taacttccca ccaaattcta
    18781 aatataattt gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat
    18841 atttttctga atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt
    18901 ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa
    18961 aatggtttaa accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga
    19021 aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg
    19081 atttttaccg gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact
    19141 tatctaaacg aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca
    19201 agagatctgc ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat
    19261 cagatggaaa gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt
    19321 tatggagcat tgcccgctct tttttataac aattttttac agaaaatggt actattgaag
    19381 tattaataag tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac
    19441 aaatgcaaat tatgagccag acgaactaga ggagacgaac gcggatcagg atacaggaca
    19501 agaatcgtct tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt
    19561 agaagactta caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg
    19621 tttatacaat ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat
    19681 cgatcaattg ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga
    19741 acaagtagaa aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga
    19801 tcggcccacg gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga
    19861 tgaagagtgg gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga
    19921 gctagacgca aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa
    19981 agaaatcaat cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa
    20041 aatcgaggaa gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt
    20101 aggctggctt ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa
    20161 gcaagaagca agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat
    20221 cagacgctat ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa
    20281 ttatcttggc aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg
    20341 aacacgatga agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga
    20401 acacttcatt ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc
    20461 tagaggaaac aaaaatacat gaaaaagaaa aagtaactcc gacgaatacg aatactgcag
    20521 aaaacaccca tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag
    20581 atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta
    20641 tgagaaaaga tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta
    20701 tattgaatga agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt
    20761 atgaattact gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca
    20821 catggaaaac aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct
    20881 tttcctttgt attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa
    20941 acgtatcacc agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc
    21001 gacactggtc agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat
    21061 tgaatcaatt attagcccat gcaagaacac ttggttttac cgttaatcag acattactag
    21121 acaatacgaa gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat
    21181 tggatgatat ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg
    21241 aaactactta tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa
    21301 aagatcaaac agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct
    21361 ttatcaatta cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg
    21421 attaccagat gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata
    21481 attgtcacaa gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag
    21541 ggatttcatt aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt
    21601 gtgtagcagg taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt
    21661 ttttatttat agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta
    21721 ttgcgatcag cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct
    21781 atactgttgt tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc
    21841 tatggttaaa gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg
    21901 tttttgatac gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt
    21961 cgtttgattt gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg
    22021 tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt
    22081 gcaatgagtg tcagtatact cattttgaca agttgcagta caacctatac acaatcggct
    22141 tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat
    22201 ggtcaaaact tttcggaaaa agaagtggtc ttcaaaaaag gcgatgattt actgagtatt
    22261 ttgaaacgaa attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac
    22321 tcacaggatg agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact
    22381 actggtgcca aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa
    22441 ctataagttt cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt
    22501 tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt
    22561 gctgctctag gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt
    22621 ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt
    22681 ttgctgttat tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt
    22741 gaaggacgag caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt
    22801 ggggcacttg gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt
    22861 aatccgatca ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta
    22921 cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt
    22981 ttgcgcgaaa gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta
    23041 atcggtttgg tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca
    23101 tttgatgcga tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta
    23161 gcaaaagaat tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa
    23221 atcaagttag cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat
    23281 ttaagtccaa cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat
    23341 gaagcttact cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag
    23401 aagatgtata tatacattga aacagataaa tatgacccaa attattctcg taaataaaac
    23461 gtttattcat gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg
    23521 ctacaattta gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga
    23581 gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg
    23641 ccactttgta taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt
    23701 ggatttgatc ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag
    23761 tttttagttg catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt
    23821 ttctttttag aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa
    23881 gaaaagcgga cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg
    23941 aactacttaa agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat
    24001 tggttctcaa tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg
    24061 attcgagtag atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt
    24121 atcatgcaga tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg
    24181 gtcgtattca tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct
    24241 tcatttgccg gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga
    24301 gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac
    24361 catgactaaa ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc
    24421 gcgcttgttc ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg
    24481 gatgaaatat gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac
    24541 tcatgaacat ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg
    24601 cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt
    24661 tttgctgtct atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt
    24721 gttcaaactg atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc
    24781 gtagggataa aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt
    24841 agagagggat ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga
    24901 aagaatagtt gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac
    24961 ctttgctaat cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat
    25021 gatggggaac aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc
    25081 ttatggattg ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt
    25141 agctgtagcc acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat
    25201 caatatcatg tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt
    25261 actagccgga atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt
    25321 atgcccatct ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa
    25381 aaatgatctg cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt
    25441 tatcttggct ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt
    25501 attgatcagt acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat
    25561 cgtcggacgc agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt
    25621 cgaaagagaa aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt
    25681 tgtcggctca gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct
    25741 tcactttttc cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc
    25801 tgtcaatcca aataaattga cattgatcct gatctccatg gtaggaacag tagctataag
    25861 cagcttgcca atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt
    25921 aggagatagt ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt
    25981 attagctgga ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt
    26041 ccaaatggct ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct
    26101 tcaatctttg aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa
    26161 agtcatcttc caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa
    26221 tggcttagca ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg
    26281 aatagcacct ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat
    26341 tatgcttgca gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc
    26401 aaaaagctat gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt
    26461 attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag
    26521 aagcaaagaa taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat
    26581 gattacgctt gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga
    26641 tatagttata aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc
    26701 agtagttaca gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt
    26761 cgttattcca atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga
    26821 agcggatgaa tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc
    26881 tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat
    26941 tttaagtatc catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa
    27001 agatgacatc agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat
    27061 gggacacatg gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat
    27121 acttgcacat atcgatcgaa tccgagataa tacctatgct tatctgattg tagatgattt
    27181 gaacaattta gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt
    27241 aaaaatcaaa ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg
    27301 ttcaagtaaa aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat
    27361 acaaccgcca gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga
    27421 gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc
    27481 agtcattggt acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt
    27541 ccttttattc aaaaaacata ccaaaaata
//
LOCUS       DAZKOK010000019        27279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000019 DAZKOK010000000
VERSION     DAZKOK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.019"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..1455
                     /locus_tag="ACG90W_000819"
     CDS             286..1455
                     /locus_tag="ACG90W_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0477203.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1928..2482
                     /locus_tag="ACG90W_000820"
     CDS             1928..2482
                     /locus_tag="ACG90W_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0477204.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2655..3092)
                     /locus_tag="ACG90W_000821"
     CDS             complement(2655..3092)
                     /locus_tag="ACG90W_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0477205.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3140..4087
                     /locus_tag="ACG90W_000822"
                     /pseudo
     CDS             3140..4087
                     /locus_tag="ACG90W_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4358..4793
                     /locus_tag="ACG90W_000823"
                     /pseudo
     CDS             4358..4793
                     /locus_tag="ACG90W_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4809..5642
                     /locus_tag="ACG90W_000824"
     CDS             4809..5642
                     /locus_tag="ACG90W_000824"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0477206.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5887..6912
                     /locus_tag="ACG90W_000825"
     CDS             5887..6912
                     /locus_tag="ACG90W_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0477207.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7050..7175
                     /locus_tag="ACG90W_000826"
     CDS             7050..7175
                     /locus_tag="ACG90W_000826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477208.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7200..8315
                     /locus_tag="ACG90W_000827"
                     /pseudo
     CDS             7200..8315
                     /locus_tag="ACG90W_000827"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8398..9294
                     /locus_tag="ACG90W_000828"
                     /pseudo
     CDS             8398..9294
                     /locus_tag="ACG90W_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9310..10134
                     /locus_tag="ACG90W_000829"
     CDS             9310..10134
                     /locus_tag="ACG90W_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0477209.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10166..11476
                     /locus_tag="ACG90W_000830"
     CDS             10166..11476
                     /locus_tag="ACG90W_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0477210.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11624..12883
                     /locus_tag="ACG90W_000831"
     CDS             11624..12883
                     /locus_tag="ACG90W_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0477211.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12893..13765
                     /locus_tag="ACG90W_000832"
     CDS             12893..13765
                     /locus_tag="ACG90W_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0477212.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13777..14607
                     /locus_tag="ACG90W_000833"
     CDS             13777..14607
                     /locus_tag="ACG90W_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0477213.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14647..16830
                     /locus_tag="ACG90W_000834"
     CDS             14647..16830
                     /locus_tag="ACG90W_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0477214.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16891..18111
                     /locus_tag="ACG90W_000835"
     CDS             16891..18111
                     /locus_tag="ACG90W_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0477215.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18108..19568
                     /gene="gtfA"
                     /locus_tag="ACG90W_000836"
     CDS             18108..19568
                     /gene="gtfA"
                     /locus_tag="ACG90W_000836"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0477216.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19645..20751
                     /locus_tag="ACG90W_000837"
                     /pseudo
     CDS             19645..20751
                     /locus_tag="ACG90W_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21073..21282
                     /locus_tag="ACG90W_000838"
     CDS             21073..21282
                     /locus_tag="ACG90W_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0477217.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21275..21424
                     /locus_tag="ACG90W_000839"
     CDS             21275..21424
                     /locus_tag="ACG90W_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0477218.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21578..22243
                     /locus_tag="ACG90W_000840"
     CDS             21578..22243
                     /locus_tag="ACG90W_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0477219.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22527..23612)
                     /gene="xerS"
                     /locus_tag="ACG90W_000841"
     CDS             complement(22527..23612)
                     /gene="xerS"
                     /locus_tag="ACG90W_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0477220.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23946..24179
                     /locus_tag="ACG90W_000842"
     CDS             23946..24179
                     /locus_tag="ACG90W_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0477221.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24176..24583
                     /locus_tag="ACG90W_000843"
     CDS             24176..24583
                     /locus_tag="ACG90W_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0477222.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25720..26238)
                     /locus_tag="ACG90W_000844"
     CDS             complement(25720..26238)
                     /locus_tag="ACG90W_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0477223.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26605..26853
                     /locus_tag="ACG90W_000845"
     CDS             26605..26853
                     /locus_tag="ACG90W_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477224.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9329 a         4143 c         5037 g         8770 t
ORIGIN      
        1 tatactacca tttgttggta gtataattta aaggtaataa gttattcttt tattactatg
       61 tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac aatattaatg
      121 aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag acaaaatgat
      181 agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag tccattctgt
      241 aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc caattttact
      301 acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt tcgatgtcat
      361 ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct tgattatgaa
      421 aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta ttcacgttca
      481 ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa tggtaagttt
      541 tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt
      601 cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga aaaaatgtac
      661 gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc tgaagatatt
      721 attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat ggatgctact
      781 cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt aattggcatt
      841 cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga atctgcttat
      901 gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt tttattagtc
      961 gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc aaatgctcaa
     1021 tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat
     1081 cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa agaagaagca
     1141 aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg agtggttaaa
     1201 ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat cagaagaact
     1261 atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata taccaaaaga
     1321 aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca attcaacaac
     1381 tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca tgatacatta
     1441 gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa aattattgac
     1501 gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa atattttatt
     1561 aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt atagtaaggg
     1621 gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt aaattagcta
     1681 ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct tagaatcttc
     1741 atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt tactgaaatg
     1801 atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt atatatataa
     1861 gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt taagaaggga
     1921 aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac aagattttca
     1981 gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt tgtttggaat
     2041 caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata tacgaggcaa
     2101 aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt ttatagataa
     2161 tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt ggttatttcc
     2221 gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat atgcagatat
     2281 agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa cttttgggta
     2341 tcactactac aagaaaacta gagacattgc taccttaatg tttattttta atcatagctc
     2401 gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag catcacttcg
     2461 tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac aaagattaaa
     2521 agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa gctggggtta
     2581 ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca tttttaggtt
     2641 ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca aacgctgcgg
     2701 aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga tagtctgctt
     2761 ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc ctctgtagaa
     2821 ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc gatatgggtg
     2881 tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata cacttctttg
     2941 acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc atgaagtgtg
     3001 atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac atagtctaaa
     3061 taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt tataggtttg
     3121 attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg tgaatcgctt
     3181 tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac taatgaaaac
     3241 aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc accaccgtgt
     3301 gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt ttggaaccaa
     3361 tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt tttgggaaat
     3421 atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg gacgacctat
     3481 cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac gcgaatttga
     3541 agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc atgccatttt
     3601 aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac gagagttatt
     3661 gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc aacgagtgac
     3721 aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc aagatcgtga
     3781 gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg gcgtagaaaa
     3841 taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta taaattcagg
     3901 aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc aattattgat
     3961 tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc aaagtccacg
     4021 ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg aacaagtgga
     4081 gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat aagtcaaaaa
     4141 ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg ttcctatttt
     4201 tataaactat cattttcgac taataaaact acttatgagt cagcctctct ttttttcttg
     4261 atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg tctcattgaa
     4321 acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata ttgaaattat
     4381 ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat aaaattagta
     4441 tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact attacccaag
     4501 aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt ttacctaaat
     4561 acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg gtcaatcaaa
     4621 agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc aatgcgttat
     4681 ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat gcttattcat
     4741 ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg attgcatttt
     4801 taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt tctgatccat
     4861 ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt tattttgtat
     4921 tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat tggcgttttg
     4981 aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat aacgtttttg
     5041 atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga gaacttgaac
     5101 aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca ttggcagtgg
     5161 aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac tacattgtag
     5221 gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta aagaaatatc
     5281 atttaacctt taaatcgcat tggatatata acaactctga atttgagcga atttatcaag
     5341 aattagctga atttactgca attgttgtta ctggagatta tttagcggct aaattagtaa
     5401 aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt ggatttgatg
     5461 atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta aatccaggta
     5521 acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa gaaataaaaa
     5581 atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac aaaatcaaat
     5641 aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat agagagaatg
     5701 aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat ttactctaaa
     5761 ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt taacgaaatt
     5821 tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat gaaaagagga
     5881 gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat tgcaacagta
     5941 tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa acgaaagatt
     6001 gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa aaatttaaca
     6061 aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc ctattttcga
     6121 acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt gaagaaaatt
     6181 attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca aggtgtgttg
     6241 attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa ttcgaatgtc
     6301 gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac tgatttaaca
     6361 aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa cattgcgttt
     6421 atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc agatgatgat
     6481 tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa atatagtcaa
     6541 atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt acttttgaaa
     6601 aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc tattgctgtg
     6661 ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat ttcgattgtg
     6721 agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac ggtgaatgtg
     6781 aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat aaaagaagtt
     6841 agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga aagtgaaaaa
     6901 aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt ttactaatat
     6961 ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt taacaaaaag
     7021 aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata gctttaacag
     7081 ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat agtaattcta
     7141 aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct ccaggactaa
     7201 gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa atggagacaa
     7261 cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt aatatgccag
     7321 atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat gctttgatgg
     7381 gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact gaaggaattg
     7441 ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct tctgtagtgg
     7501 ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca gatgaaacat
     7561 ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca aaaggcatct
     7621 atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt tatcaaaatg
     7681 ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca gaagcacaag
     7741 aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca acaattacag
     7801 aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca atgatgattg
     7861 aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa aatgttgctt
     7921 ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt ggttgggctg
     7981 ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt ttagctagtg
     8041 aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta aaaggctttg
     8101 aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga aaaatgttgg
     8161 agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa gcagaagttc
     8221 aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc gctcaaattg
     8281 aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac aatatacagg
     8341 cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg ggatattatg
     8401 aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta tatttttatt
     8461 tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca aacagcaatt
     8521 ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt aagaaattac
     8581 caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt aatttatgca
     8641 gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt aatgaataga
     8701 aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt tgctgcacca
     8761 tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact gattaataac
     8821 atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc gattgcgatt
     8881 tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt gttattacta
     8941 gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga tggtgctagt
     9001 ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt attttttgta
     9061 gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat gatgattact
     9121 cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta taaacattca
     9181 tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt agtgattata
     9241 ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc
     9301 tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat gcttatttta
     9361 ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg gaaaacgttg
     9421 gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct aagtaactat
     9481 caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt tttaatggtt
     9541 gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt
     9601 ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat gatgattccc
     9661 ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat tttaaataca
     9721 attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt attaagacaa
     9781 tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg tgccaatatt
     9841 tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt tgcacttgga
     9901 atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt caatacttca
     9961 attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata tgccacgaac
    10021 ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt gatattcatc
    10081 gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt ataaaactat
    10141 taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg caggaagtac
    10201 tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac aagatgcaca
    10261 tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa
    10321 tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga
    10381 agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc
    10441 aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta ttggtgacac
    10501 catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt tagactttgc
    10561 taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa atccgatggc
    10621 gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt tatgtcatag
    10681 cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc aatatgactt
    10741 aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt tggaaattac
    10801 aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc
    10861 acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata ttacagaatc
    10921 aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc cagaattgat
    10981 tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac aaattaatga
    11041 ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata cgagaagccg
    11101 tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag tgattgcagg
    11161 aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg tggaagtacc
    11221 ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt
    11281 agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag ccgctttaac
    11341 actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat catctgaatt
    11401 gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg gggactattt
    11461 acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc ttactctttt
    11521 cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac attagtgtta
    11581 acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt ggtctaaaaa
    11641 aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt gtggaaaaaa
    11701 tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa aagaaatgac
    11761 tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata ttcatgtaaa
    11821 agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg caggcgatgt
    11881 accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg cgaaagctgg
    11941 ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg gatatgcaca
    12001 acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg tctatggttt
    12061 ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa cttgggcaga
    12121 atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg cgattgcagg
    12181 agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg ctgctggtgg
    12241 tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta atgctgaaag
    12301 caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa aagctttgca
    12361 aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa aaggtgatgc
    12421 gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa atccagattt
    12481 tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa cgattggcgc
    12541 aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag ccaaacgttt
    12601 tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag atggtgcacc
    12661 gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg gtttaactga
    12721 acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata gtgaaaatga
    12781 tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca tgattaacgc
    12841 attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata aaatgaaaat
    12901 aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa ttattttgca
    12961 gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa ctaattggac
    13021 tggtctgact tataattttg attttgtggg tttgaacaat tataagattc ttttttctga
    13081 tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca tgattgctgg
    13141 acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta aagggaaaac
    13201 attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg tttctttagt
    13261 atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag gcattgaatt
    13321 tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa tttttgtttt
    13381 actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc aaagtattcc
    13441 acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa ttttttggaa
    13501 tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg cattaaaagg
    13561 tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa gcgatgccac
    13621 aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt ttggttatgc
    13681 gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg cacaattaaa
    13741 attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa agaaaaaaat
    13801 atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc cttagtttca
    13861 acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt tcgttttcct
    13921 aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg tcattacttt
    13981 tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat tattcctatg
    14041 gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt gatgtattta
    14101 tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat taccgttatg
    14161 atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct cacttatgcc
    14221 gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc tatcagtctg
    14281 gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa aattgttttc
    14341 ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg gttttggaat
    14401 gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac actaccattg
    14461 tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt tgcttcctat
    14521 acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag tattatagct
    14581 ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa aggagttcat
    14641 aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa taaaaaaatt
    14701 agctatctat taggaatcga agaaaataat tatttaacgc atctttattt tggaaaaaag
    14761 attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt ctcacccaac
    14821 aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga atttccagga
    14881 tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa cggtagtcgt
    14941 gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag cttagcggga
    15001 ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat tcaattgaaa
    15061 gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa tcacagtgta
    15121 attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat taaccgctta
    15181 gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt aaatggtacc
    15241 tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa agtattagat
    15301 agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt agatgccaaa
    15361 acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg taatcacgaa
    15421 acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat taattcattt
    15481 aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt cgtgatggtt
    15541 tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta taatcaccgt
    15601 ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa ctgggaagca
    15661 acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc gaagaacttg
    15721 ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa tgacttcact
    15781 tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga aaatttagcc
    15841 aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga aatgatttct
    15901 gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc aggtcgtgac
    15961 cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt tcgtgataat
    16021 atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt gaagtgggat
    16081 atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat gcaaggtgaa
    16141 gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac aacgaattat
    16201 ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc aggtttcttg
    16261 tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg tctgaaaatt
    16321 caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt ttcagcagtc
    16381 ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc tgcgatggct
    16441 ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa agcagaaatt
    16501 aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg aactttttat
    16561 cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc aaaagaccaa
    16621 acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc attagtcaca
    16681 ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt aacatttgct
    16741 ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg tgattatgcg
    16801 acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata agtgcaacac
    16861 caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt tactaaaaag
    16921 cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc aatgattaac
    16981 tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa catcaaaaaa
    17041 aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt cattgggccc
    17101 tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga gcggacattt
    17161 aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg tttagatgaa
    17221 tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac ggatattgat
    17281 gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga tatgcgtacc
    17341 aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt atatttagaa
    17401 aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat caatgattat
    17461 agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt tatttatcaa
    17521 ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt tgatgatgta
    17581 gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga gcaagcattg
    17641 aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg gaatacgcaa
    17701 aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc taattatcca
    17761 gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta ttatcatcaa
    17821 ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg acaatttcta
    17881 ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga agataaaatc
    17941 gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga tgaacagtac
    18001 caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg aaaattgcgt
    18061 ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg aaaattaaaa
    18121 acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc gaattaaaac
    18181 aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg cctttttttc
    18241 catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat ccagctcttg
    18301 gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt gattttatga
    18361 ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac catgagcagt
    18421 caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat cgaccaacac
    18481 aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa acggtagaat
    18541 ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa attgatttag
    18601 atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat atggcgaacc
    18661 atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa ttagatacca
    18721 atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt caagaagcag
    18781 cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt caaatgaaaa
    18841 ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact ctgtattcac
    18901 tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg atgaagcagt
    18961 ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat ttattaacag
    19021 atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat gtgaagaagg
    19081 tctattcaag tgccaattac aataatttag atatttatca aattaacagc acctattata
    19141 gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa gtatttgcgc
    19201 cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat attgaactgt
    19261 tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta gccgaaattg
    19321 aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa tttagaaatg
    19381 aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca gaatcagaaa
    19441 tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg gctaacttgg
    19501 atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt gttgcattaa
    19561 aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga tgacttatcc
    19621 aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc atttaataaa
    19681 tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg tatttttgat
    19741 tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt tctctacgca
    19801 gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga ccgatcgctc
    19861 caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc atttaatgca
    19921 cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt tcaacatcaa
    19981 atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt ttcgcttagt
    20041 ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa gttacactta
    20101 aactattgtt atatggacga tactacgatg tatccaactg attttacctt aacaaatgcc
    20161 catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc aacttacgtg
    20221 tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga tggctatttc
    20281 ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct ggaaacatcg
    20341 ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac ctatctaacc
    20401 gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca atttatcacc
    20461 aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg ttggcaaatc
    20521 gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt ttctcaaagt
    20581 gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct cacgtttctc
    20641 atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt ctttcgttat
    20701 ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg agaaaaaaga
    20761 aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt tctggaaaaa
    20821 gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat agttttcaga
    20881 atattctaaa atgttttatg caacactagt gatatttttt agaattagat ttctaatgtg
    20941 cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt aaatttgaat
    21001 aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt gaataaaaaa
    21061 tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg ttatgggaag
    21121 tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa aagctatacc
    21181 tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga agttttaggt
    21241 atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa aagataaaaa
    21301 tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga aaagagtaca
    21361 aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc gaacttgttt
    21421 ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag gagttttttt
    21481 ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat aaaaatcggt
    21541 tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag aaatacgtcc
    21601 aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt cttatgtaag
    21661 aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg gactacttga
    21721 aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag ttgatttttg
    21781 gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt tgtaccaaca
    21841 ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg gagaagccac
    21901 tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga ataaaacact
    21961 ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt ctagtattcg
    22021 tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg agcgtcaagt
    22081 ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc agaaatatac
    22141 aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa tccgcataca
    22201 cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg cacttcgaat
    22261 taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg gtcatttgta
    22321 tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa ctaatttttc
    22381 taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca aaagtaggat
    22441 cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg tgttgtttct
    22501 tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat cgtagccgca
    22561 ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa ttgttgggca
    22621 gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg acgcagtttg
    22681 tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg ttcgattgca
    22741 ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc tttttcgttt
    22801 tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata ttccattgcg
    22861 aatgctgcta tattgacagc atctctttta tttccttttc tagttactac cacattattc
    22921 ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct caatccagat
    22981 cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata atagatcaaa
    23041 gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata attttgtgta
    23101 tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa cgtagtttta
    23161 tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt tcctagagga
    23221 ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat atttctattg
    23281 atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt tgttgtctct
    23341 ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt taggtcatag
    23401 gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc actcagaagc
    23461 cattcataaa agagcttata attttttaga tattctaaag tggttcgagg tgattgatct
    23521 ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag tatgattgta
    23581 tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat aagtagggga
    23641 gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa ttggtatgct
    23701 ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt taataattta
    23761 aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa attttttgaa
    23821 ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca tgagagggac
    23881 tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa aggagggaga
    23941 agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta gtattaacta
    24001 tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt tttttagatg
    24061 aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa attgatttag
    24121 caatatctca agcattaact gaatatgata ctacttttag agagcttgtc gataaatgag
    24181 tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa taaaaaaata
    24241 ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt gtgtagggca
    24301 acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa aagcagctat
    24361 cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac gtactgcttt
    24421 cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg atcaaaaaga
    24481 tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc aactaaaata
    24541 tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta ctgtatgtgt
    24601 ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa atagctataa
    24661 ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa gataacgact
    24721 atttatttta tcatattttt actataccct atacttttac ttggtatatt tatgaataat
    24781 cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata ctaaagttat
    24841 ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga taacacgaaa
    24901 gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat cgaaagtgag
    24961 ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt acatcttgca
    25021 gaaatcgtta ctgggattaa actaagcaat aaaaactaat gttcccctca ttctttgaac
    25081 ttctactatt tgatttattt attctgataa atgacgattt caccttcagg atcttcaaat
    25141 cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc gataggccac
    25201 ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg gctttagcat
    25261 attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct taacaatttc
    25321 aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac ccttcatgtg
    25381 ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc tgaaaaacat
    25441 cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta gcggatatgg
    25501 caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa ccaaaattga
    25561 tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca ttcacccaat
    25621 aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt aacatacaag
    25681 aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta atctcttcca
    25741 ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat gaataaattt
    25801 atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt tgtgtgcagt
    25861 gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa tattacgatt
    25921 gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa accacttata
    25981 taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag ataatccatt
    26041 tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat atgctaaatt
    26101 ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt caggaatagt
    26161 ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta attcttcttt
    26221 atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc tttaattcat
    26281 ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat atgcattgac
    26341 tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta aagaaatgtt
    26401 gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa atcggactgg
    26461 tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca attactaata
    26521 aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga tagttcacaa
    26581 aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat aggattacag
    26641 catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa aattgacgat
    26701 gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga tcaactcgaa
    26761 gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg aactattaaa
    26821 ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta tgttgcacaa
    26881 tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg cggtgtaatg
    26941 aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat gaataattat
    27001 gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta tagattgttt
    27061 aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata ctctgttaag
    27121 ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac taacggacag
    27181 tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa atgctgctca
    27241 tttcaactta tatgttttag tgaactaccc ccacttagc
//
LOCUS       DAZKOK010000020        27239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000020 DAZKOK010000000
VERSION     DAZKOK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.020"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACG90W_000846"
     CDS             complement(56..1444)
                     /gene="ltrA"
                     /locus_tag="ACG90W_000846"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0477225.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(2013..2390)
                     /locus_tag="ACG90W_000847"
     CDS             complement(2013..2390)
                     /locus_tag="ACG90W_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477226.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            2646..3374
                     /gene="purC"
                     /locus_tag="ACG90W_000848"
     CDS             2646..3374
                     /gene="purC"
                     /locus_tag="ACG90W_000848"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0477227.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            3374..3628
                     /gene="purS"
                     /locus_tag="ACG90W_000849"
     CDS             3374..3628
                     /gene="purS"
                     /locus_tag="ACG90W_000849"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0477228.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            3630..4301
                     /gene="purQ"
                     /locus_tag="ACG90W_000850"
     CDS             3630..4301
                     /gene="purQ"
                     /locus_tag="ACG90W_000850"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0477229.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            4302..6524
                     /gene="purL"
                     /locus_tag="ACG90W_000851"
     CDS             4302..6524
                     /gene="purL"
                     /locus_tag="ACG90W_000851"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0477230.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            6509..7948
                     /gene="purF"
                     /locus_tag="ACG90W_000852"
     CDS             6509..7948
                     /gene="purF"
                     /locus_tag="ACG90W_000852"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0477231.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            7971..9023
                     /gene="purM"
                     /locus_tag="ACG90W_000853"
     CDS             7971..9023
                     /gene="purM"
                     /locus_tag="ACG90W_000853"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0477232.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            9020..9598
                     /gene="purN"
                     /locus_tag="ACG90W_000854"
     CDS             9020..9598
                     /gene="purN"
                     /locus_tag="ACG90W_000854"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0477233.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            9595..11130
                     /gene="purH"
                     /locus_tag="ACG90W_000855"
     CDS             9595..11130
                     /gene="purH"
                     /locus_tag="ACG90W_000855"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0477234.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            11146..12420
                     /gene="purD"
                     /locus_tag="ACG90W_000856"
     CDS             11146..12420
                     /gene="purD"
                     /locus_tag="ACG90W_000856"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0477235.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            12542..13456
                     /locus_tag="ACG90W_000857"
     CDS             12542..13456
                     /locus_tag="ACG90W_000857"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0477236.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(13589..15154)
                     /locus_tag="ACG90W_000858"
     CDS             complement(13589..15154)
                     /locus_tag="ACG90W_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477237.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(15147..16598)
                     /locus_tag="ACG90W_000859"
     CDS             complement(15147..16598)
                     /locus_tag="ACG90W_000859"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0477238.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(16612..17628)
                     /locus_tag="ACG90W_000860"
     CDS             complement(16612..17628)
                     /locus_tag="ACG90W_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0477239.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            17848..18936
                     /gene="chvE"
                     /locus_tag="ACG90W_000861"
     CDS             17848..18936
                     /gene="chvE"
                     /locus_tag="ACG90W_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0477240.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            18990..20516
                     /gene="mmsA"
                     /locus_tag="ACG90W_000862"
     CDS             18990..20516
                     /gene="mmsA"
                     /locus_tag="ACG90W_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0477241.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            20518..21723
                     /gene="mmsB"
                     /locus_tag="ACG90W_000863"
     CDS             20518..21723
                     /gene="mmsB"
                     /locus_tag="ACG90W_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0477242.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            21980..22957
                     /gene="guaC"
                     /locus_tag="ACG90W_000864"
     CDS             21980..22957
                     /gene="guaC"
                     /locus_tag="ACG90W_000864"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0477243.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            23221..24645
                     /locus_tag="ACG90W_000865"
     CDS             23221..24645
                     /locus_tag="ACG90W_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0477244.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(24687..24944)
                     /locus_tag="ACG90W_000866"
     CDS             complement(24687..24944)
                     /locus_tag="ACG90W_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477245.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            25106..26215
                     /gene="pdhA"
                     /locus_tag="ACG90W_000867"
     CDS             25106..26215
                     /gene="pdhA"
                     /locus_tag="ACG90W_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0477246.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            26218..27195
                     /locus_tag="ACG90W_000868"
     CDS             26218..27195
                     /locus_tag="ACG90W_000868"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0477247.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8625 a         4770 c         6043 g         7801 t
ORIGIN      
        1 aactgacctt ccgcccctct cacaccaccg tacgtacgga cccgtatacg gcggttcaat
       61 aagttgagtg tacgaactgg tacttcaagg agatgtcctc atatccttgg cgggccagtt
      121 ctttgtctgt taatgatcga tgaagaatat gacttttgga gactgaccaa ataccttttc
      181 ttgtatttga ccattcatac gctttcgctt tatcgatccc taaacgaatc agattttttc
      241 ttttggttct tggattcttc cattgtttcc aaatatattg tctgattctt cgcttcaacc
      301 aaccgtttaa ctccctcatg aattctttca tctcaccaat tccatagtag ttgatccaac
      361 cagtcattag ttgcttgatt tcttttaaaa taagttggat acttcttcca cggtttcttt
      421 tagtgagttg tttgagcttg cttttcacac gctgttttgc ttttgcgtga gggcgaattt
      481 taataccttt ctttgttggc agaatgcaga atccaagaaa ctttcgtttc aatggacttc
      541 ctaccgcact cttttctcga tttaccgtca actttagttg ttcttcgaga aattgaatgg
      601 atttcttcat cacacgctgt cctgctcgtt tgcttctaac atagatattg cagtcatcgg
      661 cgtatcgaac aaatttatgt cctagatttt ctagcacttg atcaaattcg ttcaggtaga
      721 tgttacttaa caggggagag agattgcctc cttggggcgt tccttcttca gttgttgtga
      781 tgagtccatt ttccattaca ccactcatta agtattgacg gatcagtttt aggacttgtt
      841 tgtcctttac tttccgctca atgaaataca tcagcttatc gtgatttacg gtgtcaaagt
      901 aggctttcat atcaatgtcc accacatatc ggtaaccttg ttcataataa gattttgctt
      961 tcttcactgc gtcatgtgcg ctgcgatttg ggcgaaaccc ataactatta tctgagaatt
     1021 ctggttcaaa tagcggttgt aacacttgat tgattgcctg ttgaagcata cgatcaataa
     1081 ccgtgggaat tcctagtttt cttacaccgc cgtttggttt cggtatttct actcgcaaaa
     1141 caggctgagg tttatagttc ccttggcgta aattggatag taattcctcg cggtgctctt
     1201 tcaagtaagg aagcagttga tcgacggtca tcccatctgc accagccgcc cctttatttc
     1261 tgactacttg aagatacgct tgattgagat tattccgatc tagaattctc ccaaacagat
     1321 tttctacacc atcttgtttc ctctgttcac cgcaagaagt gctgcgcact tcatcatacc
     1381 cttcgtgttc cacactagct ctttgatgat agccaagttt tcttgttttc tgcggtcgtc
     1441 gcatcttctc acacctccta aattattcaa gattcttatt gttcagtcct tcgtaacggt
     1501 cagtgtcgtt actactatga cctcggctga cttctcatga ttcaccatca tgtcgccatg
     1561 atcgttgtgc tggtttgttt tcattcaacg cccacaacac cctcatgaga tctcccccgg
     1621 taagagtgaa aactttcctc ccatgtaact gctagattta ctgtataggt ttcgggcagt
     1681 attggacttt gtcttgtgat gaagacttat ccgacctaat tcagccttgt atctagtttc
     1741 tgttcgtcag ttcaggagtt tgcgtccgac ttccttcagc cacaacctca cgattgccgc
     1801 cttgtctttc gctaacagtt cctactgcca agcctgtagt ggactttcac caccaagttt
     1861 tcacccatgc agggcgcaca ccaaaaaaga agcagatccg ttagcggttc tgcttctttt
     1921 agtttattat attttcttat caataatagg aacctgattt taaaagcggc tacctcttta
     1981 ccgtgcctct ttacatacaa aaaatagagg aattaagcgt aaatggcttg gctattataa
     2041 ctaaaatgct ggttatggag atgttgcagc aagttttcga gaaaagcgta ttccatctct
     2101 tgtccattga ataagatctg gatacgataa tgatttttga aacgcttctg ttcatgaatc
     2161 tgactgattg aaagttcgcc tttgtgttct gtctgataaa gaaaagtcct tttacggcat
     2221 tcagcacagc gcttggttgc tcggcggatt tttcggaatg tataaaaact agtatcttta
     2281 ttgcataaat aaacgtcttt gatcgacagc aaatcattat atagacagga acgttcttca
     2341 gctgttaatt ctgataaaaa aagctgacct tgttttattg catcttccat cggcaagtgc
     2401 ctcctcgaac ccattatcca aatttttagt taattctaat ataccatcat taagtataaa
     2461 tgtaaacgct tgttgatggt ataagatttt cgtttctgat aaaacacgaa cattaaaaga
     2521 atctaatgcc ttaaatgtac atatttttgt tgatttataa aaagatgatt gctaaaattt
     2581 aaagtgaaaa aacgaacaaa aaacgatgta tttgctttta atgtaaataa aaaggagaga
     2641 cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc caaaaaaata tatcttactt
     2701 ctgatgagaa cttgatttgg atagaatatt tagatcaagc aactgcgttg aatggcgcga
     2761 ggaaagacca gataagtgga aaaggagaat tgaataatca aatcacaagt gcaattttcc
     2821 gttatctgac tcaaagagga ataaaaaatc attttattga acaattgtct aaaaccgaac
     2881 aattagcaaa aaaagtacaa attatccctc tggaagtggt tatccgaaat attgcagcag
     2941 gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg
     3001 aattctacta taaggatgac cagttggatg atccatttat caatgaagag catatctatt
     3061 tcttacaact tgcaaagccg gaagaaatca gtgagctgaa agcccaagcg ttggctatta
     3121 atgaaatttt gaccgaatta tttgctaagg tcggtttggt tttagtagat ttcaagctag
     3181 aatttggccg gtcgaatgga caaattttgc ttgcggatga agtaacaccg gatacttgcc
     3241 gattatggga tgaacgtacg aatgaacata tggacaaaga cgtttaccgc agagatctag
     3301 gtgatttgat cccagtctat caagaagtat ataaccgtct atcggaacta actgaagaaa
     3361 aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc tataaagatt cagtgttaga
     3421 tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga
     3481 tatccgaata ggaaaatact ttgagataca acttgctgat gcagaagaac caatcgatac
     3541 agtgattgaa acgatttgtg acaagctgct tgcaaatgta aatatggaaa cataccgata
     3601 tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt
     3661 caaattgtga cgcagatatg ttatgggcag ttcgcgagat catgggcgct gatgccgaat
     3721 ttgtacggca tgatgagtcc tctttagaag gatttgatgg ggttttactg ccgggaggat
     3781 tctcctatgg tgactatttg agatgcggtg cgattgctcg gttttctaca atcatggaag
     3841 aagtgattcg ttttgctgaa gaagggaaac cagtattcgg tacatgcaat ggattccaga
     3901 tattgacaga agcaggactt ttgccaggtg tactgagacg aaatgagtct ttgcatttta
     3961 tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc ctttacttca gagtatgaag
     4021 ccgacgaaat aatctatctt ccagttgctc atggggaagg aaattatttc tgtgacgaag
     4081 aaacacttca agaactaaaa aaaaataatc agattgcctt tacttataat gacaacatca
     4141 acggcagcgt ggaaaacatt gcagggatta cgaacaaagc agggaatgtg ttaggaatga
     4201 tgcctcatcc agaacgtgca gtagaagact tgctaggatc agaagatggg aaacggttct
     4261 ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac
     4321 cgacagctga agagatcaag aaaaaaagaa tctatgccga atggggattg actgatgaag
     4381 aatatcgaat gatcgaagaa gatatcttag gaagaatgcc taattatacg gaaactggac
     4441 tgttttctgt tatgtggagc gaacattgtt cctataaaaa ttctaaaccg gttctacgca
     4501 aatttcctac tagtggacct catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg
     4561 acatcggtga tggactagca gttgtcttca aagcagaaag tcacaatcat ccttcagccg
     4621 tggagcctta tgaaggagct gcgacaggag ttggcggaat catcagagat attttcagca
     4681 tgggggctcg tccaattgcg ttgctagatt cattgaggtt cggcgaatta gataatccaa
     4741 gaacgaaata tttactagaa gaagtagttg caggtatcgg cggatacggc aattgtatcg
     4801 gcataccgac tgtaggcggc gaaattgcat ttgatccttg ttatgaagga aacccattag
     4861 tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat ccagaaaggg caagccgctg
     4921 gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg tcgcgacggt atccacggag
     4981 caacttttgc ctcagaagaa tttaatcaag aagaagaaca gcaacgttct gctgtacaag
     5041 taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg tttggaatta atcttgcatc
     5101 attcggattc tctaataggt atacaggata tgggagcagc aggacttgtt tcatctagtg
     5161 cggaaatggc ttcaaaagct ggctcaggac ttatattgac attagatgaa gtcccacaaa
     5221 gagaaactgg gatgacacct tatgaaatga tgttatcgga atcacaagaa cggatgttga
     5281 tttgtgttaa atcaggagag gaagaaaaaa tccaagaact atttcagaag tatgatctag
     5341 atgcagtcac tatcggtaaa gtaacagacg acgggcaata tcggctatat catcaaggag
     5401 aagaagtagc aaatctgcca gtcgatgcat tagctgaaga tgcaccagtt tatcataaag
     5461 aaatgaaaga accagctcga atcgtagaat ttcagcagtt agctccttat cagccagtta
     5521 tcgaagaacc tggacaagta ttgcttgatc tgctgcagca accgacgatt gcttcaaaac
     5581 gttcaatcta cgaaacttat gattcacaag tacaaaccaa tacagttgtc cgtccaggaa
     5641 gcgatgctgc tgtgatgagg gttagaggga cgaataaagc gttagctatg accacagact
     5701 gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg acaaatagca gtggcagaag
     5761 ctgctagaaa tattattgct agcggtggta agcccttggc gatcaccgac tgcctgaact
     5821 atggttctcc tgataaacca gaagtttttt gggaattatc gacttcagct gacggaattg
     5881 ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg aaatgtctct ttatataatg
     5941 aaacggatgg tcaagcaatc tatccaacac caatgattgg tatggtcgga ttgattgagg
     6001 atcataaaca tatcactact caggaattca aaaaatcagg ggatctaatc tatatattag
     6061 gtaagacgtt tgcagatttt gatggcagtg aattacaaaa gatgcagctt ggaagaatcg
     6121 aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag aaaccaagag ttagtattga
     6181 cagccatcca aaacggattg attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa
     6241 ttgcactggc agaatcagct ttcaaacatc agctagggat atctgtacag tttgaattgt
     6301 cctctgctca attatttgca gaaactcaat caagatttgt gttgacagtc gcaccggaaa
     6361 ataagacacg attcgaagaa atgatgggag atgctgctgt tttagcaggt aaggtgacgg
     6421 atgaagcgat aatcgaaatt tcagcaactg acggtcagat caaaatagaa actgcagtag
     6481 caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga
     6541 atgcgggatc ttcggtgttt ggggtcatcc agacgccgct agtgtggcat acttcgggct
     6601 ccatagtctt cagcatcgag gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa
     6661 gcttaagggg catcgagatt tggggctgct ttctgaggta ttcaaagatc aaagaaaatt
     6721 gcagcagtta acaggagaag ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg
     6781 aagtgtggac aatattcagc cttttctatt caaatttcat gatcagtcga tcggcttagc
     6841 gcataatggt aacttgatca atgctcgttc ccttagaaaa gagttagagg aaaatggggc
     6901 gatcttccat tctaactccg atacggagat attgatgcat ttgatccgac gaagtgaaga
     6961 aacagatttt ttaaataaat taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta
     7021 tttattaatg acagaaacgg caatgattgc cgctttggac ccaaatggtt ttcgtccatt
     7081 agcaatcgga caaatgaaaa acggagcata cgtgattgcc tcggaaactt gtgcattaga
     7141 agttattggg gcacgtttta taagagatgt ccagcctggt gagatagtga tcatcaatga
     7201 cgaaggaatc cagattgatt gctttacgaa ggacacgcag ctttctattt gttcgatgga
     7261 atatatctat tttgccagac cagattcaaa catcgcaggg atcaatgtgc atactgcacg
     7321 gaaaaatatg ggacggaatt tagcgaaaga atcgccagta aaagcagata tggtcgttgg
     7381 cgtacctaat tcttctttgt cagccgcaag cggttatgcg gaagctagtg gaatccctta
     7441 tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca tttatccagc caacgcctga
     7501 gctaagagag caaggtgttc gaatgaaatt atctgctgta cgaggagtag tggaaggtaa
     7561 aaaggtgatt atggtcgatg attcaattgt tcggggtacg acaagcagac ggatcgtcca
     7621 tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg attgcttctc ctcctttgaa
     7681 atatccatgt ttttacggga ttgatattca aacgcgtaaa gaactcattg cggcaaatca
     7741 cacgattgat gaaatcaaag aatgtatcgg tgcagattct ttaggttttt taagtgaaga
     7801 gggtctgatc gaatcgatcg gtctggaaaa agatgctccg tattcaggat tgtgtatggc
     7861 ttatttcaac ggagattatc cgacgccgtt atatgactat gaagaaaaat atcaggaatc
     7921 actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag aagggagtat atgaattcaa
     7981 tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc tggatatgaa gtagtagaac
     8041 ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat gatgggcgct ataggaggat
     8101 tcggcggttg ttttgattta tcggtctttc aattaaaaga gccagtcctt gtctcgggga
     8161 ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc agacaaacac gatacaatcg
     8221 ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc acaaggcgca gagccgttat
     8281 atttcttaga ctacttagca acggggaaaa atgatccgtt acgtttggaa aatgtagtaa
     8341 aaggtgtagc agatggatgt gttcaaggaa atatggcgct gattggcgga gaaacagctg
     8401 aaatgcctgg tatgtactca ggtaatgatt atgatctagc cggatttgct gtagggatag
     8461 ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa
     8521 tagcatctag cgggattcat tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc
     8581 agcagcagtt ttctttatca tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat
     8641 tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa
     8701 taaaaggagc tgcacatatc actggtggag ggtttgtaga aaatatcccg cgaatgttac
     8761 cagaatctat ggctgcacaa atcaaacttg gctcatggcc gattcttcca atattcggct
     8821 gtttacaaaa atatggtgaa attcctgaga tggagatgta tgaaatcttc aatatgggca
     8881 tcggtatggt cctggcagtc caagaatcga aagtaaaaga aatagtacac gaaattgagc
     8941 aagctggcga aaaagtttat gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg
     9001 tgtttatagg agaagaacga tgagaatcgc tgtttttgct tctggaaacg gaagcaactt
     9061 tcaagcgcta gccgattatc tatcgaaaaa aggattggaa tcttcgattg actggttgtt
     9121 ttgtgatcag cctgaagcat atgttttgaa acgtgcaact gcgttgagtg taccggcaga
     9181 ttgtttttca cctaaagaat ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa
     9241 attgaaagaa aaaaagatcg atttgatcgt attagctgga tatatgcgaa tcattggacc
     9301 ggtattgctg aaaaattatg ataaaagaat catcaatatc catccgtctc ttttacctgc
     9361 cttccctggt ttgcatggca tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt
     9421 cacaattcat tatatcgacc aaggtgtaga tactggacca atcatccggc aggaaaaagt
     9481 gagaattgaa caggaggaca cgttcgattc tttagaagaa aaaatccata gagtagagca
     9541 tcgaatctat ccagaagtga ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca
     9601 agagcattga tcagtgtatc agataaaact gggattgtag cattcgccca aggtttgatt
     9661 gaacaaggag tagaaatcat ttcaacagga ggaacaaagc atgcgttgga taaagctggg
     9721 atcaaaacgc tttcgatcga agaagtgaca gggtttcctg aaatgatgga tggccgtgtc
     9781 aaaacacttc atcctaaaat ccatggtggt cttttaggaa gacgcgatct ccctagccat
     9841 atggaggcaa tggaaacgga aaatatccaa ccaatcgact ttgtttgtgt caatttatat
     9901 cctttcaaag aaacgattat gaaaccagat gtcgaaatag cagaagctat tgaaaacatt
     9961 gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa accatgaatt tgtcacctct
    10021 gttgttgatc catctgacta tgaagaagta ctttcagaat tacgtcagta tgggcatact
    10081 tcactttctt tcagacaaaa actagcggca aaagtctttc gtcatactgc ggcatatgat
    10141 gcactgatcg ctcaatactt aacggattgg gtggaagaaa aagaaccgga aaaattgaca
    10201 cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa atagccatca agccgctgcc
    10261 ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag cagcaaaaca actccatggg
    10321 aaagaacttt cttataataa tatccgagat gcagatgctg cgctacggat catgaaggaa
    10381 tatgacgagc caacggtagt cgccttgaaa cacatgaatc cttgtggtat tgggacagca
    10441 tctacgattt tagaagcgtg ggaagcagcg tatgaagcag atcctgtttc aatctttggt
    10501 gggatcatcg ttttgaatcg agaagtagat ctgccaacag cagaacagat gcataagctg
    10561 tttctagaaa tcatcattgc cccttcttat acacaagaag ctttggaatt actgcaaaaa
    10621 aagaaaaaca tccgattact gacagtagac tttgcgcagg aagaaaatca aaatcgagaa
    10681 gaaacggtct cagtcttagg tggacttctt gtacaagatc aagatctggc aatagaaaat
    10741 gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc cagcagaagc aaaagcatta
    10801 gcgtttgctt ggagagctgt caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa
    10861 cgtcagacag taggaatcgg agccgggcag atgaatcgag tcggttcagt aaaaattgcg
    10921 attgaacaag cgcaagagaa aatggaaggt gcagttttag ctagcgatgc ttattttcca
    10981 atggatgatt cggttgaata tgcagccaaa catggtataa aagcaatcat tcagccagga
    11041 ggaagcatca aagaccaaga ttctattgat atggcgaata aatacgatct agcaatggtc
    11101 tttactgatg ttcggcattt tagacattag agaggagtcg gataaatgaa cattttggtg
    11161 atcggtgcag gtggtcgtga acatgcaata agtaaaaaac ttttagccag tccgaaggtg
    11221 gatacggtgt attgtgcgcc aggaaatccg gggatgacaa aagatggtat caattgtgtc
    11281 gctatcagtg agcgagatca ttcagcgtta atccattttg ttaagaaaga ggctattgat
    11341 tggagtatcg ttggtcctga agttcctttg ttaaatggga tcgtcgatga ttttatggcg
    11401 gaaggattga aaatcttcgg tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt
    11461 tttgctaaac aattaatgga tacttatcaa atcccgacag ctaaatccag aagttttgaa
    11521 tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg
    11581 gacggcttag cagctgggaa aggtgtagtc gtggctacca cagtgaagga agcgctggat
    11641 gcggcagaac aaatgttgaa ccagcatcga tttggggata gtagcaaaaa aatcgtcgta
    11701 gaagagtttt tggcaggaga agaattctcc cttttggcgt ttgtccgtaa taccaaggtg
    11761 tatccgatgg tcatttcgca agatcataag cgggcatttg ataacgataa agggccaaat
    11821 acaggcggaa tgggtgctta ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa
    11881 gctgtggaag aagttttaaa accagcggct ttgggaatga aagaactagg acgctctttc
    11941 acgggtgttt tatatgcggg tttgattgct acgaaggaag gaccaaaagt gatcgaattc
    12001 aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt
    12061 gcgcaagtaa tcgatgattt attagagaat cgtacagtag agttaaagtg gcagcaagaa
    12121 ggatacaatg taggcgttgt ggttgcagct gccggttacc ccgaagaata tgagacagga
    12181 atggttctac cagattttct ggaagaagag ctttctgttt actatgctgg agtttcctca
    12241 aataacggca aactagttgg agcaggagga cgtctttatt tagtagaagc atacggtgag
    12301 acgatcaaag aagcacagaa aaaaatttac accgcgttaa tggataaaac gacagatgga
    12361 acattttatc gtacagatat tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa
    12421 atcagctgct tccatttata aaaaatacaa caaacataat cggaagcgcc gattttagtg
    12481 aagccttttc ataaagagtg tggtacactt ttgttgtgta tataaataag gaggagttca
    12541 gatgaaaaaa attcaattag gtacttctga tcagaaagca gctgcagtga tcttaggctg
    12601 tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt gagacagctt atgaaaacgg
    12661 cattgatttt tttgaccatg cagatattta tggaaacggt gaatgtgaag aaatctttgc
    12721 agatgctcta gcaaaaacat caatcaaacg agaagatctt tttattcaaa caaaatgtgg
    12781 aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat atcattgaat ctgtagaaaa
    12841 aagtttgacc cgtctgaaaa tggattatgt ggatgcttta cttcttcatc gtccagatac
    12901 attggtagaa ccagaggaag tagctgaagc gtttgatgaa ttggaacggt caggaaaagt
    12961 tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt
    13021 tcgtcaacca ttagtagcta atcagctgca atttggcttg aaacatactg gaatgatcga
    13081 tcaagggatt catgtcaata tgacagatga agcaagtatc gatcatgacg gtagtgtact
    13141 tgattattcc cgattgcatg atatgacgat ccaagcatgg tcaccatacc agtatggatt
    13201 ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga
    13261 actagcagct aaatatcaaa cgacaccaac tggcttggct tctgcttgga ttttacgcca
    13321 tccagccaat atgcaagtga tcgccggtac gatgaacgta gggcgaatca aggaaatcgc
    13381 tcaagcttca gaaattaata tgtctcggga agattggtac gaattgtacc gtgctgctgg
    13441 aaacatttta ccttaaagat tgtcataagc aggtcaaaag ggctataaat taaaaagaca
    13501 gaataacttg aaagctgttt tcagcttcga gatattctgt ctttttttat tgcaaatgga
    13561 aagagtatca ttgtacggat ttcgtttatt agttattttt gtttttaagg aaaagctgtg
    13621 tccgatattc ttttggagaa atcccttgtt ttttcttaaa taaataactg aaatagttag
    13681 ggtcattata accaatttca aaagcaatat ctgaaatacg atagtctgat tgctgcaata
    13741 atttttttgc taattcgatt ctttgttctg tcagataatc gatgaatgtt tggttaaacg
    13801 attgagaaaa aatcgtactg aaatgagagg gacttaatgc aacttgttga gcgacccaag
    13861 ttaaggacat atctggatcg gtaaaatttt ctttgataaa atccaatgca tggttcaaaa
    13921 ttggctgata tttgttcctt gaaggctgtg agggcagagc agttaagaaa gaaatgaaca
    13981 tataaactgt cgaacggtaa agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg
    14041 atgtgcaatg aaccatttct tgcgggattt tcttttcttt caggcagaca tgctggagta
    14101 gcttagtcaa gacaaataaa cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg
    14161 tcaagtgttc gatgtactta gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga
    14221 ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc
    14281 tgataatttg ttctttaagg gaactggcgg tttcaagtat catctgcgtg atctcaaaag
    14341 atgcaggtaa ttcgctgatt ctttctgcga caggaccaat ggcgacagaa aaattatttt
    14401 tttgtccctt ttctaattca tggaccaacg tttgggctac ctgataactg gtctcgatca
    14461 cattttcttc aagagaatca actattaaaa atttgatgaa tcgagaggaa acagcagaga
    14521 agatcacttg ttgattctcc ctaaataaaa acttcaaata atcactgaga tgagcgtaat
    14581 cttcaaagtc tgtactgact tggtttgctg ctaataatac ggtcattttt ttccctgcta
    14641 aagagcgatt gaaagattca gcaagtttca atgtttcact gatcgtaagt gagcctttga
    14701 acaaaccgtt caagaaatga ttttttttca attcgaaaac aagatcagaa gaagtgtgcg
    14761 tttctttttg ctgcaaatct aaagcagtta ctgctttttc tattgcttgt ttcaattcgc
    14821 tacttttaat cggtttaagc agataatcat ctgcctgtac ctggattgct cctttcatat
    14881 aatcgaaatc gtcgaatcca ctaataaata tgagacgagt ccaaggaagt agtttccttg
    14941 cttcttttga aaaagtcaac ccgtccatga aaggcatttg gatatcagtc agaatgatgt
    15001 cgggctgtaa gtcgataatt gaagacagtg ccatctcacc atctccagct tcgccgacat
    15061 aagtgattgg ataataagaa ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga
    15121 ggtattcgtc ttcgatgata aaaactttat acatgtttgt ttcctccaat tgttttggga
    15181 gatagaggga tcatcagtgt gacagttgtt ccttctcggt atttgctgtc aatcgtgata
    15241 ggtttttgat catttccgta ataaagaagg agacgcttgt ttacgttgta aagtccgtag
    15301 ccgatactga aatctgaatc gatccctttg gctaactctt tacgtatttc tgccagtttt
    15361 tctgcaggga tgccaatccc gttgtctata actttgaatt ggatcatctc atcaattttg
    15421 catgtggtaa tacgaataaa tcctttgtga cggacgtact tcgttccatg gtatacagca
    15481 ttttcaatca atggctgcaa gatcattttt aaaatagaaa caggaaaaag tgtttctggt
    15541 atatcgatct tatagtcaag catatggccg tatcttattt ttaagatgac tagataatca
    15601 ttgatatggc taatttcttt ttcaagagga atccagtctt gtcctttgct taaagacgta
    15661 cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt cttcaaagca gtcttgttcg
    15721 atcaaagaaa taatggcatc caaactgtta taaataaaat ggggagtaat ctgagcttgc
    15781 aatacacgaa cttcactttg tgctaaatgg tattgtttca aagcgttctc ccgtaaagac
    15841 tggtttagat cttccgccat tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt
    15901 tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa gttgttctga catctcgatc
    15961 agctgattga tcggttcttg gacctttctc tctaaaaagc gacgattctt tttcgcatag
    16021 aaaaaaatgg tactaagaac tccaagttcc actattgtta atagaatgag cgagcggaca
    16081 agtgtcgtat tttttttgct tgctaagtcg atctccacac gaacaaaatc ttgtaagata
    16141 tctgacagca gttgagtgac agaatctacc tgattgatga tctcttcatt ggtatccata
    16201 aaatcttttg ccaagatatt attgatcagc tcgttttgat aatcttccag tgtatcgatc
    16261 gtacgtagcg atacatctaa cgtactaatt tctttaagtg tatttgtatt atttttgatc
    16321 tctcgaatat cattcctaag ttcttctagg atattttcct cattgaactc atctacggtt
    16381 acttggccag aaatcaagtc ccacatactt tccaaaactt tttgatcaac cttcaaagca
    16441 attgcatctg ctttttggat atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa
    16501 tataacagta tgctcagtag caaagggata acagcaaata gaagcatggc tttgtttgct
    16561 tgggcaaaaa tctgttttat gcttaaattt tttgtcatga tacttctcca gtcaataatt
    16621 tctggttggg atgttagcta tattctgctg ggaaaagaca gtctcggaaa taaaaatatc
    16681 tttaggtaat gtttttcctg tttgaatatt tccaaaataa cgagtaacgg tattggctac
    16741 ataccagccg acatcaggtt tacactcgac tacacaattg acgattcctg agcgtagctt
    16801 ctcgatcata tcttcttgtc catcaatagt tacaatgatc aaatcttctc caggtttgat
    16861 tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt tcatcactat ggctgtataa
    16921 tacatcgatt tcggataaat catttgtttc taaatatcgt ttcatctggt cttttccttt
    16981 catccgaata aaatcaccga caattcggtg aacaatagtc atcggaggtt tatggtcgat
    17041 ggttccatct ttttttaggg ttggtttacg attgattgat tccaaaaatc cttcggagcg
    17101 tagcttggtc ggtgtggagt tctctgaacc agcaagttct aggattctga tttcctgttt
    17161 ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa
    17221 actggggcca atgtgggtga cgaaaagctc gggatcattc gtttgaatat gtctgtcgac
    17281 aagtatgaca gggatccctg ctcgctttgc ttcttttaat acagagtccc acccggattc
    17341 ttgcaaagga gtaaaaatga tcatatccac tttataggca atgaacgtcc gtatatcttg
    17401 gatttgccgt tcctgattca tgtagccatt tcggtagaga acttggtaat cttctttttc
    17461 aagcgcagtg ataattgatt cagtatgctt tttccgccag ctactctccg ttcctgattg
    17521 cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta ttaggtgtac accccatcat
    17581 ttggtaagcg attacaacaa gggctaaaaa aaataaaaca cgtttcactt taaatcactc
    17641 catctggatt tgttagtact atcttatcag ataaaaggga aaatcaaagt caaaaggaat
    17701 caatcgaaaa aaatccaaga aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa
    17761 atttaaagta aatcccataa aaaaatcaat gttaacgctt tctttattag ctatactcgg
    17821 tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat ttagaaagat tgttgggact
    17881 atcgctattt tgaccgcaag cattctatta agtgcgtgtg gcaattcagg atctgcagat
    17941 gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat cagcagaaag atggatcgct
    18001 gatgggaaca acatggtctc ggaattagaa aaactgggtt ataaaacaga tcttcaatat
    18061 ggagaagata aagtagaaaa tcaagtagca caaatagaaa atatgattac aaaaggggta
    18121 gatacacttg tgattgcttc tatcgatggt tcggctttaa ctgatgtgtt agctaaagca
    18181 aaagaagcag atatcaaggt gatcgcctat gatcgtttac tgatgaattc agaaaatgtc
    18241 gattattacg cgacttttga taattttgga gtaggtgtat cgcaagcagc ttatattgaa
    18301 gagcacttag gattgaaaga aggaaaagga ccattcacga ttgaattatt tggtggctcg
    18361 cctgacgaca acaacgcact aatcaattac aacggagtga tgtctgtttt acaaccatat
    18421 atggacaacg gtcagttggt cgtgccatct ggacagacga gtttcagtca aatcgctacg
    18481 cttcgctggg atggttctac ggcgcaagca aggatggata atctgttaag cgcaaattat
    18541 actgaccaaa cattggatgc tgttttgtcg ccatatgatc caataagttt aggaatcatt
    18601 tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac ctttacctgt gattactgga
    18661 caagatgcga cagttgctgg cgttaaatcg atcattgccg gggaacaaac acaaacgatc
    18721 tttaaagaca cacgtatctt agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat
    18781 ggagaagagg ttccggtcaa tgatactgaa acttatgata atggggtgaa aactgtccct
    18841 acgtatttag caaatacagt atctgtcgat aaagataatt atcaagcaga gctgatcgac
    18901 accgattatt ataaagaatc cgatctgaaa aattaagagg aaccaaacaa ggaactaaag
    18961 agatctaagt cagagagagg ataagaatta tggcggattg tattttaaaa atggcagata
    19021 tcaccaaaaa attttctgga atccgagcgt tagattgcgt gaatctgatg attgaacgtg
    19081 gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa atcaacattg atgaatgtct
    19141 tgagcggctt gtatccatat ggctcctatg acggggaaat cttttatgat ggaaagctgt
    19201 gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat cgtcatcatt catcaagaat
    19261 tagcactaag tccttatctt tcaataaaag agaatatttt tctcggaaat gaacaagcag
    19321 aaagaggcgt gattgattgg gaactgacag aaaagaaaac atatgaactg ctgaataaag
    19381 tcggtttgtc tatcgacccg aatcttctag tatcccagat tggcgtaggg caacagcaac
    19441 tagttgaaat agctaaagca ttatcaaaat ccgtccgtct gttgattttg gatgagccta
    19501 ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag attgatccga gagttgaaag
    19561 agcaaggtgt aacctccatc attatttcac ataagctgaa tgaaatcgtg gatatcgcag
    19621 atcgaatcac cattttacgg gatggacaga cgattgagac actagaaaat gaaatgatca
    19681 gcgaagagca tattattcgc ggaatggtgg gcagagattt agttgatcgt tatcctgaaa
    19741 gaaaaccgga tattggagaa gtttatttta aagtagaaga ctggacggtc tatcaccctc
    19801 ttgatcacga gcgaatcata aacgaccaca tcaacttgca tttgaaaaaa ggagagattg
    19861 taggaatagc tggactgatg ggagcgggca gaactgaatt cgctatgagt ctatttggac
    19921 attcttatgg aagcaagata tccggacgta tattaaagga aggggcagag atatcagtta
    19981 agaccgttcc ttcagccatt gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt
    20041 taggactgaa tctgctgatg gatattcggg aaaacgtgac gctgagcagt ttggataaaa
    20101 taagtcaaaa aggagtatta gataaagaaa aagaagtaac tgaggcagaa aatttccgga
    20161 aaaagatgcg tataaaaaca aactccatcc atcaaattgt cagtagtctt agtggtggga
    20221 atcaacaaaa agtcgtttta tccaagtggc tgatgacaca acccgatatt ctttttttgg
    20281 atgagccaac ccgtgggata gatgttgggg caaaatatga gatctacacg attatcgaag
    20341 aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc tgaacttcct gaaatattag
    20401 ggatgtgtga tcgaatctat acgatgaacg aaggacgtat tacaggagaa gtctcgcgag
    20461 aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa agaagaggtg ggttaagttg
    20521 gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac taaatttgaa aagtagagca
    20581 gctgatcttt ttggtcgcta cagcatggtg atcattttag cgggactact tattgcgttt
    20641 caattgatga cggatggtat tttctggcgt ccattgaata taactaacct tgttctgcaa
    20701 aatagccata tattagtact agctgcaggg atgttattag ttgtgctctt aggtcatgtg
    20761 gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc
    20821 aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc
    20881 ggcgcatggc aaggtttttg ggtagcttat gcagggatac cggcgtttat cgtgacttta
    20941 gcaggattgc tgatgtttcg tggactgaca caagttgttc taggaggcca atctctagca
    21001 ccttttccag ctgttttcca aaaaatgtct acgggttatt taccagactt aagcgaagga
    21061 agcagccttc acttcttgac attgatttta ggaatgatca tcacgatcgt gttagttgtt
    21121 ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat ttgaagtgga atctccaaca
    21181 gctttcttgg taaaggcggt atttacagca gtcgttgtac taggaataac ttatatcttt
    21241 gcctcttatc aagggtatcc gattattttg atcattcttg gtttgatcgt tgcagcatat
    21301 gggtttttga caaataaaac agtagcaggt cgacagattt atgcgacggg cggaaatcga
    21361 aaagcggctg aactatcagg gatcaaaaca aagcgaatca cgttctgggt attcgtcaac
    21421 atgggagtca tggcagcttt agctggatta gttttagctg ctcgattgaa tgcggcaaca
    21481 cctcaagcag gtacatcgat ggaactggat gcaatggcgg cagtatattt tggtggtgct
    21541 tcgacttccg gcggcattgg aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt
    21601 gtattgaaca atggcatgtc gattttaggc gtcggggtag actggcagca ggcaatcaaa
    21661 ggattgatct tgcttttagc cgttgtgcta gacttgtata ataagaaaaa acgaacaacg
    21721 taggagaacg ccggttattc agaaaaaaga ctgtgacaag acatttgtca cagtcttttt
    21781 ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac taaaaataat atatgttttt
    21841 ttaccattcg tataaattct ctttttgctc ttgatttttt tgcttaattg agttaaatta
    21901 catagggcgc taaaaaaagc cttgattaaa ttaataatgt tcgtgttttg ctggatgtga
    21961 acatcaggag gataatgtaa tgaaagtatt tgattacgaa gatattcaat taattcctaa
    22021 taaatgtatc gtaaatagcc gttctgaatg tgatacaacg gtcacattag gtaaacatac
    22081 attcaaaatg ccagtagtgc ctgccaacat gcagacgatt attgatgaaa caatcgcaga
    22141 attcttagcg gaaaatggtt atttctatat catgcatcgg tttgatgaag cagctcgaat
    22201 tccgtttatc aaaaaaatga aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa
    22261 gaaagaggaa tattccttta tagagaagtt ggcagaagaa tcgctaaatc ctgattatat
    22321 cactatcgac atcgcgcatg gacatgctaa ctcagttatt gatatgattc agcatattaa
    22381 aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg
    22441 tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg attggtccag gaaaagtatg
    22501 tattacaaaa atcaagacag gctttgggac tggaggctgg caactggcag ctcttagatg
    22561 gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt gggatcagaa cacatggcga
    22621 tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg attggttcat tgtttgccgg
    22681 tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga atcgtgtata aagaatactt
    22741 tggttctgct tcagaattcc aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg
    22801 gatccaacac aaaggatctt tgaaagatac actagtggaa atgcaacaag accttcaatc
    22861 atctatttct tatgctggag gaagagatct ggaagccatt cgaaaagttg actatgtcat
    22921 cgtgaaaaat tcgattttta atggagacac aatctaaaaa ataaataaaa tattaggatg
    22981 ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc aaacaaaaat agttctttat
    23041 atgttttgtt tggctggaat tattatcgca gatacaagct tttgaacatc attattcaga
    23101 ataagggact gtgataagat ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc
    23161 ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt
    23221 atgtgggaaa aagtagagaa aaaacaagga ccgatttatc gtcaaattat ggaacagatt
    23281 atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc aacttgaatc agaacgaaaa
    23341 ctagggcaac tttttggtgt gaatcgttcc accgttgtcc atgcattgga agagcttagg
    23401 gagcttgggg tactaacgag caaacgtggc agcggatggt ttgttaatca gactgaatgg
    23461 gggaaattcg ctgttccaag aatcgattgg cggcaaatga tctctcctcg ttatgagcag
    23521 acggactggt atgagcaaaa aataagagaa gcaaagcaga tccaaaaaac atcgtttctt
    23581 gatttatacg ctagcgagat gccagatgag cttttgccga attttgagtt tccttcttat
    23641 tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc ttttaggcta tactccgtta
    23701 cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat tggcattttc ccccaaccag
    23761 cttcttgtga ctggtggcgg tcagcaagca atctttttga ttttacaaac gattctttca
    23821 gctggagagg ggatagctgt agagtcacct tctttttttt accgattacc tcttttcaaa
    23881 gcttcaggaa cgcgtttatt tgggataccg atggatgaag aagggatcga cttagaaaaa
    23941 ctgaaagaat cgatccataa aaataaattg aaagcagtgc ttatcaaccc gaattttcaa
    24001 aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga
    24061 cagtatcggc ttccaattat tgaagacgat gtatttacgg atctttcatt tctaaaccaa
    24121 gaagaagtcc ttcctatacg aacgattgat ccggataatg tgttatacgt cggttcgtta
    24181 tctagggttt taggaaaaac aacgaaaatc ggttgggtga taggacctga gacttttatc
    24241 tcgcaactag ctcaagcgca agaaatgatg gagttttcga tgaatatcct tacacaaatc
    24301 acagtggcta atgtatttaa tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag
    24361 ttgaaagaaa aaagccgact gataagcgct tggggagatt cacaatcgtt tttcaagctt
    24421 cattcaattt taggtggtta ctatgcctgg attacttggg atgggaaaaa actagacccc
    24481 gaactagcag aaaaagtagt agatatgggt cttggaattg caccaagtct actttttggg
    24541 caagatataa atggaatcag aatcaattac agtaggctgg atgaaaacca aataccgttg
    24601 ttttattcaa agatgaaaat tctagaaaag tggttaacag aataatttca caaagtaaat
    24661 agattaaaga atataacagc ttggatttaa atttcaagct gtttcttttt tttcggaaaa
    24721 agaatacgat ataaagaaac taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt
    24781 aaataaaata aatgtttgca acaaaactgt ataacaccaa cgtttttaaa tagaaacaga
    24841 tggttgtaag tttgattaga aaatagaaaa aatgcagatg aaatataaaa cgctttcctt
    24901 attgtgattt tattcactaa tgctgttaat atgcggattg gcataatttc ctttcatcgt
    24961 gaaaatttgt gcttttagct tgtatttctc ataaatccat gataatttat taatgaagac
    25021 ggttaaatag tgatacagtt ttattcactg tttcaatcgc agaatgaaga gattttctta
    25081 tagatgagag aggaatgtgt gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc
    25141 actgctagat gaagtcaatg cagatttccc agtatatcaa gcacttgatc aagatggaaa
    25201 cgtagttaac aaagatttag tacctgattt atccgatgat gaactcgttg aattaatgac
    25261 tcatatggta tggtctcgtg tattagacca acgttcaaca gcgctgaacc gccaaggacg
    25321 cctaggcttt tttgcaccaa ctgctggaca agaggcaagt cagctggcta gtgcttacgc
    25381 atttgataaa gaagatgttc ttcttccagg ttatcgggat gtacctcaac tagtaaaaca
    25441 tggtttacct ttatctcaag cttttttatg gtctcgagga catgctgcag gtaattctta
    25501 tccggaagaa ttgaaagctc taccaccaca aattatcatc ggtgcacaat atgtacaagc
    25561 agccggtgtt gcattaggtt tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac
    25621 tggtgatggc ggttcatcac aaggtgattt ttacgaagca atcaatttcg ctggcgctta
    25681 ccatgcaaat gccgtatttt atatccaaaa caacgggtat gcgatctcta cacctcgtga
    25741 aaaacaaact gcagcaaaaa cacttgcaca aaaagctgtc gcagctggga ttcctggtat
    25801 tcaagtcgat ggaatggatc cgcttgcagt atatactgta tctaaaatgg cacgggaatg
    25861 ggcgatcagt ggaaatggac cagtcctaat cgaaacactg acttatcgtt atggaccaca
    25921 tacactatct ggtgacgatc caacccgcta ccgtacaaaa gacacagatg acgattggca
    25981 gaaaaaagat ccattgatcc gcttccgtaa atatttgacg gaaaaaggac tttggtctga
    26041 agaaaaagaa gaacaagtca tcgaagcaac taaagaagaa atcaaagcag ctattgctga
    26101 agccgacaaa gtaccaaaac aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc
    26161 tccacaatca atcaaagaac aaattgcaat ttatgaagcg aaggagtcga aataatcatg
    26221 gcacaaaaaa caatgatcca agcaatcaca gatgccttag cacttgaact agaaaatgac
    26281 gagaatgtcg ttgtttttgg tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact
    26341 gaaggattgc aggaaaagtt cggagaagac cgagtattcg atactccttt agctgaatct
    26401 ggaatcgcag gattgagttt cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc
    26461 caattctttg gttttatttt cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca
    26521 agatatcgaa tgagcggcac tagaaatcta cccatcacaa ttcgttcgcc atttggtggc
    26581 ggtgttcata caccagaatt gcattcggat aatttagaag gtttgatcgc tcagtctcct
    26641 ggtattcgag tagtgattcc ttccaaccct tatgatgcaa aaggactatt gatttcttct
    26701 attagaagta acgatccggt tgttttccta gaacacatga aactatatcg ctcattccga
    26761 gaagaagtgc cggatgaagc ttacgaagtt cctttggata aagcagcagt tactcgtgag
    26821 ggaacagatg tatctattat cacatacgga gcaatggtgc gtgaagcaat caaagcagca
    26881 gacaatcttg caaaagaaaa tatatctgtt gaaatcattg atcttcgtac agttgcgcca
    26941 ttagatgtag aaacgatcat tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa
    27001 gaagcacaac gacaagcggg cgttgccgcg caagtagttt ctgagatctc agaacgcgct
    27061 atcctttcat tagaagcccc aatcggacga gtttctgcac ctgatacagt cttcccattt
    27121 ggacaagcag aaaatgtctg gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa
    27181 atcgcggaat tttaagaaaa gaagggacga aacaaagtca tggcttatca atttaaatt
//
LOCUS       DAZKOK010000021        27173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000021 DAZKOK010000000
VERSION     DAZKOK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.021"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..1615)
                     /locus_tag="ACG90W_000869"
     CDS             complement(47..1615)
                     /locus_tag="ACG90W_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0477248.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1619..2029)
                     /locus_tag="ACG90W_000870"
     CDS             complement(1619..2029)
                     /locus_tag="ACG90W_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0477249.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2026..2949)
                     /locus_tag="ACG90W_000871"
     CDS             complement(2026..2949)
                     /locus_tag="ACG90W_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0477250.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2946..3824)
                     /locus_tag="ACG90W_000872"
     CDS             complement(2946..3824)
                     /locus_tag="ACG90W_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0477251.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3811..5148)
                     /locus_tag="ACG90W_000873"
     CDS             complement(3811..5148)
                     /locus_tag="ACG90W_000873"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0477252.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5151..6254)
                     /locus_tag="ACG90W_000874"
     CDS             complement(5151..6254)
                     /locus_tag="ACG90W_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0477253.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6328..7836)
                     /locus_tag="ACG90W_000875"
     CDS             complement(6328..7836)
                     /locus_tag="ACG90W_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0477254.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7802..8494)
                     /locus_tag="ACG90W_000876"
     CDS             complement(7802..8494)
                     /locus_tag="ACG90W_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0477255.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8516..9490)
                     /locus_tag="ACG90W_000877"
     CDS             complement(8516..9490)
                     /locus_tag="ACG90W_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477256.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9790..10662
                     /locus_tag="ACG90W_000878"
     CDS             9790..10662
                     /locus_tag="ACG90W_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0477257.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11120..13318)
                     /locus_tag="ACG90W_000879"
     CDS             complement(11120..13318)
                     /locus_tag="ACG90W_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477258.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13550..14542
                     /locus_tag="ACG90W_000880"
     CDS             13550..14542
                     /locus_tag="ACG90W_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0477259.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14602..16023
                     /locus_tag="ACG90W_000881"
     CDS             14602..16023
                     /locus_tag="ACG90W_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0477260.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16016..17515
                     /locus_tag="ACG90W_000882"
     CDS             16016..17515
                     /locus_tag="ACG90W_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0477261.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17554..18948
                     /gene="uxaC"
                     /locus_tag="ACG90W_000883"
     CDS             17554..18948
                     /gene="uxaC"
                     /locus_tag="ACG90W_000883"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0477262.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19107..19790
                     /locus_tag="ACG90W_000884"
     CDS             19107..19790
                     /locus_tag="ACG90W_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0477263.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19792..20628
                     /locus_tag="ACG90W_000885"
     CDS             19792..20628
                     /locus_tag="ACG90W_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0477264.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20639..22393
                     /locus_tag="ACG90W_000886"
     CDS             20639..22393
                     /locus_tag="ACG90W_000886"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477265.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22669..23010)
                     /locus_tag="ACG90W_000887"
     CDS             complement(22669..23010)
                     /locus_tag="ACG90W_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0477266.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23078..25249)
                     /locus_tag="ACG90W_000888"
     CDS             complement(23078..25249)
                     /locus_tag="ACG90W_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0477267.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25460..26317
                     /locus_tag="ACG90W_000889"
     CDS             25460..26317
                     /locus_tag="ACG90W_000889"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0477268.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26388..>27173)
                     /locus_tag="ACG90W_000890"
     CDS             complement(26388..>27173)
                     /locus_tag="ACG90W_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0477269.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         8067 a         5608 c         4886 g         8612 t
ORIGIN      
        1 aactagtgaa aataagttag tgaacctcat ttattaaaga attaaattac agatgatctt
       61 ttccatcata cttgaaaaac tcaaaatcag cataacttcc tcttttttta tttagatcat
      121 gcactgctat cccgataaaa tttccagtaa accctcctga gagtatacac atgttttttt
      181 gcggacctac tttcttccat tcttcagttg atacgtcttt caaatgaaac tgtgctgtct
      241 ctttctttac agtgactttc aaaaaagtct tcttttctgt gagtggaatc gtccaatctt
      301 ctatttgtgc tactccgttt tctgattcta tcatacgtag gcattttccc ttgtcctcat
      361 tccaagtaac ataacaataa atataattgg attcatttaa gtacagtacc agtccagcca
      421 tctgattaaa attattaggc atgtattcaa atgctgtctc cgcatcaaaa gtaaaatccg
      481 tttgccgaat agctaacaaa tgatgttcga aggtagattg aagagattca ccggaaatca
      541 gacgtaaaaa acctggtcta cattttgtat cacaccaatc agcatttggc aaaatgcgtc
      601 tggcattcca gttcttcttt agctctcctt caaatgtatc cataaactga tgatcttttt
      661 cttgttgtac ctcttcattt gtctcaatca ttatttcttc catcggagtt gtctggctat
      721 ctttaagtct taaccaatcg tcggacgtcc aatacacttc ttgaatagcg gtttctcttc
      781 ccaaaatagc cgcaccgttt aatggtctgg tacaaagata tgtcatgtac catcttcctg
      841 acggggtttg tactaagctc ccatgtcctg tacattgcaa tgtagaagca ggtttgtcac
      901 tagctgtcag cataggaaaa ccaggatcaa gctcgtaagg accagtaata ttctttgctc
      961 ggcaaactgt aactgaatgt cctcttcctg taccgccttc tgccgtcatc aaataataat
     1021 acccgttacg aaaatacaaa tgtggtgcct ccgttttcgc taattctgta cctgcaaata
     1081 ttttatagat gggaccatca agcttcttct tctctgcatc atattcttgt aatacgatac
     1141 cgctggattt attcggtgtg tccagacgat aatcccatag ttcattgaca agccattttt
     1201 ttcccgtttc aagatcatgg aacaaagaag gatcaaagcc gttactattt aaataaattg
     1261 gttcggacca aggacctgtg atctcagatg cagtgatcaa ataattgtgg acatctttaa
     1321 acggacggcc tgcacttttg acatctgtgt aaacaagata aaacaatcca tcagcataac
     1381 tgatctgtgg cgcccatata cttccatttg taggatttcc ttgcaaagat acttgagtgg
     1441 tcaaaatatc cgttgtatgt gaccagttga ctaaattttt cgactcatat actcggattc
     1501 ctggaagcca ttcaaaagag gagacaataa tgtaataccg ctctttcact cttaatatat
     1561 taggatctgg attgaatcct cttaaaatag gatttttcac tttgatagtc tgcatgattc
     1621 aggctccttt tctcattctt actcgatata aactgaatgc cccagctgcg aaataaaaaa
     1681 atgcaaaagg aaacgcaata aactgagtgc caatcaacaa tatgaatcct gttataaaat
     1741 acaaaccagt ggcagttgtt aagtattttc ctttagcagt caaaaacaat gctactgcta
     1801 aaaaagcaag tgaaataaac aacacaacgg caaacagacc agtcatgtcc tgtaataatc
     1861 caaatgtatt ttctaaagac aaagttgaat gatctacagc aagcgactga taactttttt
     1921 taaactctga cgcagacata ttacttaaag taactgctaa cccgccaaag aaaatcaaag
     1981 atagactgcc accgaccatt cctagcaata gttcccattt tcttttcatt tctgaacccc
     2041 ctgcttttct aaatgtatcc attcaggtct tttgatcgat gtttcataat tgttattacg
     2101 ttcagagaat ttgacagtct gttcattttt cttgtcatcc caattatccc aaccagttgg
     2161 gtgaacatgc ccacctatct tacacgcttc aaataagaca tttgcatacg gacgccacgg
     2221 tcttcctaaa tagacgtttc tagtatcagg ttctgcagtc aaggtacagc tttcaaaacg
     2281 ataccctgtt ttttgatttt tcggcgtaga tgctgctgtg atgtatccag gatttttact
     2341 ggctaagcta tggatatgac actgatgaaa aacggcatct gccccaccaa atatgaaatc
     2401 caccgttcct tcaatccagc aatattcata ggtctgacga caatactcag gattggcaga
     2461 agccggtgta caaaaaggag taccatcttt ttgcaaatca ggcaacggac ccgtgcataa
     2521 ggtatcctga taaccagata gtcgacagtt ttttaaagat acgcgatctc ctgaattgaa
     2581 taaagcaaca gcctgtccta ccttttctcc tggacctgct gtattttcga tgtgaagatt
     2641 ttctaatacg acattcgatc cttctaagaa aaaagtagca cttcggaaag tccctaactg
     2701 gctaccgtca agatactcct gttttgcaaa acgattaccg gagataacta ctgttcctgc
     2761 tcccacaagt ttaaaattgg ataatctgct ttctatatat tcatcatatc ttccttcttt
     2821 tactatcaag acctttggat ggctatccgg caggcctgcc aaataatcga ttccttcttg
     2881 gatagtttca aaatccgtcg gggtatctat agcattcttt ccaattatca gtttgtcttt
     2941 catcctcata aatgctccct tatgaattgt ttccaaatat ttctcatttc ttgtgtttcc
     3001 atatgaccgc ctgttaaaga aaaagaagaa aagttttctg gactacccat tgattgatac
     3061 tttttgtcca gcccttttct cagcttcagt acgccttcgt ccaagcacat cgtatcgttt
     3121 tttccaacta agctaagacg agggcgcgga gcgatttttt cttggattgc taacgttgta
     3181 taagctgtca atagatttgg cacatagtaa taaaacccat gatgatctag gcggcgatgt
     3241 agcaaaagta tttcgatgtc cacctgtgcc gctagatcaa tacaaacttt gacacgagaa
     3301 tccaatgctg tcacccacca gcttaataag cctcccatag aaagaccaat cgttgcgagc
     3361 cgttgagtat ctgtgtcttc ccttgtctcc aaataatcga tcaagctgat cacatcataa
     3421 atacgcattc cccataacgt ttttcctgtt aataaaaatt gtttgaacaa ctcgctttca
     3481 gaaatacctc cgcgttcttc aaatccccaa gcatcaatcg accaaacagc atatcccatt
     3541 cctgtcagta cttctgcaaa agatggagaa gctaagtaag gagcaccatg gatcaattca
     3601 cttttcccga ctgaaaaatc tccgccgtga gaatgcaggt aaaggaccgg cggaaacggt
     3661 ccttcttttt ctggataagc atagagggct ggtactgttt ccaattcatt taagtacaat
     3721 tgatagtatt caagcataaa accgttttgt ttttctcggt ataatcgttt tccactaggg
     3781 gtatgtctgc tagagggagc acctaaaaaa tcattcaact gcataagcaa agtttttacg
     3841 aacaaccagt tcatccatat tttctgattg atttgattgg aatttctcaa tactgatttc
     3901 tttagtattt tctaatagaa atgctggtcc ctcgtgattt tcaatcgaca tctggttaaa
     3961 ggaaacctta cccgcaaagc caatataaaa tccttgtctg ctcatttcct ctattcctgt
     4021 catcatagca ggaacaccgg gaactgcatc ttgggccatc gagacatcga tattgtcaaa
     4081 tgtgatttct tgtatgaatt gttccgctaa tccataaata aaacctgcag aagcatggac
     4141 attccttgcc gtgatattcg aaaaatggat ccttctgaat gctggtgtcc gttcatctat
     4201 cggataagcc tttttttccc atacatacgg ttctttccct cgcggaccac agaaataata
     4261 aagattcagt ataaatggac acatcacatt atccatgact atattggata ctcgaatatc
     4321 ttcaacgatc ccgcctcgac ctcgtctaga tttcaatcgt attcctcgat cagtttcttg
     4381 gaagatacaa tttgaaatgg taatattacg aatactacca ctcatttcac ttcctaatac
     4441 gacaccgcca tgtccatgga ccatcgtgca atttgtgatc gtgatatttt cgcaagcgat
     4501 cctttcgtac gtatcttcag tccctgcctt gattgcaata cagtcatctc ccacgtcaat
     4561 atggcaatta ctaatacgca catttttgca tgattctgga tcaattccgt ctgtatttgg
     4621 tgaatccgct gggttcaaga tcgtcaagtt atcaaacgtt gcattactgc aaagaatcgg
     4681 gttgatcgtc caacttggtg actggatcaa tttaatatct ttcacagtaa ttcgatgaca
     4741 gttatggaaa ctcatcaatt taggacgagg ataagcgaga ttatctggtt catttctaaa
     4801 agtatgccac catttttttc catttccatc taatgtaccg aaaccagtga cagagatgtt
     4861 ctctacattt tgagcataga tacaggaagc ataaactttt cgatggactc cttcccaacg
     4921 tgaatgaaca acaggataat cttttggatc gtcagagaat ttcagaaccg ctcctgcgct
     4981 taaatgaagt tcgacattgc tttttaaaaa taaagctcct gttagaaatt cacctgctgg
     5041 aacaactacg gtcccgcctc cgtcacttgc tgcagcatca atggcttgtt gtattgcttc
     5101 agtattcaat tcatcaattg atgcgccaaa tgtcaaaata tcatacatat ttataatgcc
     5161 tccacttcaa tcaatgcttg caagaatgca ccaacgcctt tttgatcatt acaaataatt
     5221 ggttcactga tgtagtaagc atatgaacca tctcgctgat ctgctccgcc taaccctgct
     5281 acttgacagt ttttattcaa gttcagccag ccttcttttg tttccagaac aaattcatca
     5341 agtaatcctt gatgtgattt cttcacttgt ggaagccatt cttctttgtt cagcagtccc
     5401 aaacgtattc ctttagccat tgcgtaaaca aacatactac tgccggaagc ttccagataa
     5461 tttcctttcc gttttgtttc tgttgttact tgataccatg tcccgctgtt ttcttcttga
     5521 tatggaagta atgcagccag tgtcttttct agaatttctt tcagcaaaga acaatctgtt
     5581 tcttcatgaa gtaattcata ggtatctgct gcagccatca aataccagcc aatcgatcgg
     5641 ctccaaaaat gatgggatag acctgttttt ggatcagccc atggttggac cttcttttca
     5701 tcccatgcat gaatcagcaa atttgttttt ggatcgaata gatgtttata actgatttca
     5761 aactgtctca aaacatcttg gtaatctttt tcttcactaa aattctggat aaattcagca
     5821 taaaaaggag aacccatata taagccatcc aaccaaattt gataaggata aatttcttta
     5881 tgccaaaaag ctccttcgga tgtgcgtgga tggtttgcca actgctggga aaggagttct
     5941 gctgcttttt tatatttttc ttcgcctgtt tctttgtaca ataaaaagaa aagctttcct
     6001 gtatttacat ggtcgatatt gtattcttca acgctgtagc cacggatcgt tccatcttct
     6061 tgcacgaaat agtcgatatt gtcttttatg aaatcaaaat aaattttttc ttgggttcgt
     6121 tcccaaagta attggaaccc tttaagtacg acgccatagt cataagacca ttttccttta
     6181 taccactttt cttcatataa gcgaggtgtt ctttccatga tactttttgc taatttcttt
     6241 gaccagtttt ccattatttg ctctccttta aaaattaaaa taggatttgg gacaaatacc
     6301 aagaagtatt tgtcccaatc gtctatatta ctgtgccgct gctttgtatg cttcctcgta
     6361 ctcttgtgtg attcgatcgc ctccttgtgc tttccagtct tctacagctt ttttgaatcc
     6421 atcttcgtca agctgtccca tgatgtattg caccgtagca tcaatgacga ttttttcaat
     6481 ttctgatcct acttcattgt tcgtcggtga atccaatggc acagaaggat ccattacagc
     6541 aaattcttca ttttctttga tcaattgatt agagaattct ttttcagggt ctgtatcttt
     6601 gaaagagaac gtcacttcac taggacgaga gcttgataac ggttgcacct cttgttgcca
     6661 aagatccata tcgataaatt cgatagaacc atcatcattc aattcataat gcgttccttc
     6721 gataccgcca gtcatcaatt tatacacatc ttcttctaaa agatcatcaa caaattgaag
     6781 taatttcttc aattgtgctt cgtcttttac ttgagatttg ggaaatgcta gcaagccgcc
     6841 aataccattt ccttctgacc aaacatgata accgccgcct tcaccatctg agatcttgtt
     6901 tacaggcact aattccatat catcttgaat accttgaccc atcgtcttca gattcttgat
     6961 atccaccatt ccagtgtaga ttccagttct tccttgtgca aattgttctt gctgatcagt
     7021 cttttgggta actgcaaagt cttgtgccaa atagccattc tcaaatagat ctcttgaaaa
     7081 attcaaagct ttcatatact cttctgtatc aaattctgga gtgaatttgc cattatcgtc
     7141 tacagcccag ccatttggtg ctccaaaata agaagtgaaa agtttaaagc tggtataacg
     7201 aatatcacta ggcgcacgat cgccaaaacc aactgtgtcg tccacaccat ttccatcagg
     7261 atcgttttct gtaaattgtt tagctacttc ataaagctca tccatggtag ttggaacatc
     7321 tagtcctaga ttatccaacc agtcttttcg aatgactaag ccagcacgag cgtagtcttt
     7381 ttgtatcggt acgccgtaca aggcaccgtc gatactcgca gcttcgattc tatcttcaga
     7441 aatttgactt aaatttttat agtcttttaa atattctgat acatcccaga atacgcctga
     7501 ttttaacgca ttgcggacag aagagttttt caacatcgca atttgaagtg taacgatatc
     7561 tcctagttca tcagaagcca atgcagttgt taatctttct tcttttgaag catcaggaat
     7621 ccagttgaat gtcagttccg tatcggtttt ttcttcgatc aagttcacaa tcgtgtctgt
     7681 aggcggtgaa gcatgatgca aaatatttaa ccaagtgatt ttagcttttc catcttctgt
     7741 ctctgaagaa ctgttacttg aactgctgcc gcatgcggca agtgctagaa ttcctgcaaa
     7801 actaagacct aattttacga gcgttgcttt tttcataaat tttctccttc actgttttaa
     7861 taatattttt gttttcttct gttttttctg tatatgagtt cttccattcc aaatggcaaa
     7921 atatagaatt tgctgtccac gtgactgcgg aaaagaagag actgccaccg ataaaggtta
     7981 aaacacctgg cattgtttgg atgatcatca gataaatccc taccatactt aatatcaaca
     8041 ctaatgagta atgaagatgt gagaatccga ataaaaatgc cattttcatt ttgaaaaaat
     8101 atcctttcca atcataatga gccatgattg ggaaaatata gagtgctgag agcacatata
     8161 tgaacgcagc aatatacaaa gcgatcctaa gataccaatt gtgtatgaaa gaaagatcca
     8221 caagtaaaac aatcccgatg gcgccatata tccagctcat cagcaaggtt tcacgatagt
     8281 tttctttaaa atatgtgaag tacgttttcg tcaaaggaaa cgtttctttt gagcgcagcc
     8341 attttctaat cactgcaacc atcgcatacg atgcaggacc gaaagtaaaa actcccaaac
     8401 ctgcgatgac taaaccgccc cacagaaaat tcaaataaac caactgtaaa atacgattta
     8461 gccttccgtt tattttatct aatgactgca gcatatcttt ccctctcttt ctatcttagt
     8521 aaacgccgtc ttctcctagc tttttggcaa tcttattagc taaaattacc ataaacaatc
     8581 ctacgacccc tttaaacaat cccattgctg tactgaaact taactgtcca ttcttcaatc
     8641 cagctgtata aatataggtg tcgaaaattt ctgcaacgct tcgattcaat gaattcaata
     8701 acaagtacat atgctcgaac cctaagtcta atgtctgacc gatttttaga atcagcaagg
     8761 taatgattac tggacgaatt gccggcaatg taacatgcca tgttttgcgt aagcgtcctg
     8821 ctccatccat ttctgcagct tcatacaact gtgtatcgac tgcagttatc gctgaaaggt
     8881 agatgatcgt agaccagccc aactctttcc agatcacttg tccgatatat actgtgcgtg
     8941 tccattcagg ggatgttaga aaactgattt tttcaaatcc cattgttgca aggaattcat
     9001 tcaatacccc tccgtcaaca ttcaaaaata cataagtaat cgaaacaatg attacccaag
     9061 acatgaagtg agggatgtaa atgatcgttt gaatcccgtt tttcaatcgt ttgttcgtta
     9121 cttcatttaa tagtaaagac aaaatgattg gcatcggaaa aaacacgaag atatttaatc
     9181 cgaacaagat caacgtattt cttaataatg taaagaatgt cggttctgta aacaaacgaa
     9241 tgaaatgttt gaaaccgacc caaggacttc ccattattcc taaaaatggt tgataatctt
     9301 gaaaagcaat aacgagtccc cccataggaa gatacctaaa aatcaagaaa taaaggactc
     9361 ccggcagaat catcacatat aaaaatttat tcgttttgat tcctgcaatt attttttttc
     9421 gtcttctgag cttttcttct tttttcgata gcgtcagttg aaatggttct tgtttcttta
     9481 cactttccat aagcccctcc ttagttttgt gaatgtttgc tgcttatgac ttcatgatag
     9541 ataactatct gaattacgta tataatcatt tttaccgtaa gtaaaaacgc ttacatagca
     9601 atgatttttg tgctatacaa aacgataaag tgtatttttt cacaaataaa ctacgcatag
     9661 caaaccttct atttttttct atctgacact tagcatagga aatgattata tacgtaatct
     9721 ttcaccgatg ataaattgaa catatagaaa cagaaacatt taatttattt tgaggaggag
     9781 cctatctata tgaaaaagaa agtctctata ggagaaagaa gttttacact tttcaacaat
     9841 acgtttctaa ttttgctagc tttgatctgt atcgttcctt ttttaaatat catcgctaca
     9901 tcttttgctt ctacacaaga agtcgtcgcg aaaaagttta tcctttttcc tactaccttt
     9961 tctttagacg cgtatcgcta cattctttct acgccgacca tttttagagc acttgctgtt
    10021 tcgattggtg ttacaggtgt cggaacgatc gtcagtatgt gtgcaacttc actgatggcc
    10081 tacggtcttt ccagaaagta tttgtttggt cgaggtttcg tcaactttct tgttgtattt
    10141 tctatgttgt tcagcggggg gatgattcca acgttcttag ttgttcgttc tctagggctg
    10201 gtcaattctt actgggccat gatcctgcct gtggctgtta atgcaatgaa tatgatcatt
    10261 atgagaaact ttttccaagc cttgccggat agtttggaag aatcagcaaa aatggatggt
    10321 tgcacagatt ttggtgtatt tttcaaaatt atgctgccat tagcgttacc gtctattgct
    10381 actatctcgc ttttttatgc tgttacttac tggaacactt atatgactgc tattttgtat
    10441 atcaacgatt catctaaatg gccgatacag attcttttac gccaaatcgt cattgtatca
    10501 agtgggatgc aagcagaaag tagtgccgtt gatgtgattc caccagcaca aacagttaaa
    10561 atggctgtta tcgttattgc aacagtgcct atgctgattg tttatccttt tgttcagaaa
    10621 tattttgtca aaggagcact cgtaggttct gtcaaagggt gattatgtac tgaacgcaaa
    10681 atggtagttg tgtcagaagt ggtcagcttc aagaaataag aaggcattta cgaaaattgc
    10741 ttcttaaatt tttgtgaaat ttcggcttat ttttcgaaga atctactcct ggaacaccgt
    10801 gtatcagagg ttgtgaaaga gacgttcagc cttgagcgat gataaaaact ctcacaaaca
    10861 ttgcgacgag taacccacag gagcaataag aagcgatttt gaaaaatagc ttcccatatt
    10921 tcacaaaatt atgccttatt gtcgatagcc tgtctcttga acgccattta ttcgtaaaaa
    10981 agaactgtga caagactttt gtcacagttc tttttgcgtg cttagccttg aaatttaact
    11041 atccataaat acaaatacta gggttattct aacttctttt attgcttgaa tcagtccaca
    11101 agaaaaacag cctcttgatt cacaaatatt tttttcgata ttctcccggc gtaatcgatt
    11161 ccttcttttt aaagaaacga ataaaatttt gcggatttcg atattgcaat ctttctgcaa
    11221 tctcttttac ggagaggtta gtctctttca accatttttt tgctacctct aaacgatgat
    11281 tttgaacaaa atcaccaaat ttttctcctg tttccttttt aaatacatta cttaaatagt
    11341 tagggttata atgcaaccta tcagcgatca catctaaaga cagatcacga tcatattctg
    11401 tgtagacgat atgcataata ttctccgaca aatttttaaa ttcatgatca gtgattgctt
    11461 gagagcattc tgtgattggc agaactaatt gttctaaaac aagtttttct agttctttgg
    11521 gatgataatt gttcagtgct tttacataaa tttgtttcat gttatcaaag ttttttgaat
    11581 ctactcccaa taactgtcct aattgaacga gttcattgat caagcggacc gaaactactt
    11641 cccgactcaa aggattttta ttttgtgcaa acaatgcatc aatcaattca tgtactaaat
    11701 tcttcacttc ctgatttcca gaacgaatgg cttcaaacaa tcgattttgt tcttctatcg
    11761 gatatctaat aagtgttttt tcatgagcag caggaacgat ttcctgataa aaaataattg
    11821 aacttggacc agtatttaca cgataataca gggcttcttt tgcccgatca accgattgct
    11881 tgctttcatt cagcgtttcg aaccggttac tgatgcctat actgatagtt aaattcaagt
    11941 attcttttac acgtttttga atgagtcggc aatactccat gacttttttg cttgcttctg
    12001 catcatcttt atccattcga taaatagtgg cttgcatttc ttcattcaaa acgatcggca
    12061 gcattcgatt ttcctttggc acaatttccg tgatcatgtt gttgatcgct aaaagtagca
    12121 aatcacgctc cccaccgtga ctatcttcta acagatcgat ttgaaccaaa gctgtataat
    12181 agcagtcatt ttgagaacta taaccaaatt gctgcaaccg ctgatctagt tctcgccttt
    12241 ctactcgatt tctaaataaa ctaagtacaa atagcgtttc cagctgtggc ttttgggtaa
    12301 ctaagtgtgc acttaacgct tctttttctc ctatgatccg actgactgat tgagcgacca
    12361 aatctagttc tttcccactg aatccttttg atttaagatt cagacgttcc tgtatttgag
    12421 aaatcggacg ggcaaatctg tccgaaaata agtaggaaag aagaccaatg agtcctacca
    12481 gtgttaaaga cactgcaact agcccgattc tcagattgcg gatcattgcg ccaattgcac
    12541 tagagtcaac ttctaatctg taaatccaat tattataatc tgatttcagt actatatacg
    12601 ttttatcttt ttctcttatc actttcagct gattgtaatc tagatccacg tccgttaata
    12661 cagttggttg ctctttattt tttactgtct gagtagtaaa aaggttgcca acttcgtttt
    12721 caatagaaag tatgtcttct ccactttcac caacaacttg ttctattgta tgtttctcta
    12781 tcgtagcaat tcccaatgca tatttttctt tttcaaacat tggcaaagaa atcaccattt
    12841 cgattccttt gtttttcttt tgccaaaata aattattttt gttatttatg tacttctgtt
    12901 gatacttttc aatttcctct tcggtcagtt gactcaacga tccattgatt actccccatt
    12961 taccagccaa actgactaaa tcataattat tatcatccat aacaatcgtt tctataaaat
    13021 ttagttcttt cctgatttca gaataagtgg agtaatctgt atatgtcagc ggatgcttga
    13081 atatgtccga gaaatttttg gtcgaaccat acgtagaaaa cgaatattca atcgttctaa
    13141 ttttccattc aacgttagtt ttaacttgcc tgagcaaatt ctttttgtct gttaaataac
    13201 tcgattcgat cgattcatag gtattctggt aaatgaataa actaaatcct atgacaagta
    13261 cggctcctaa taaaaacatc ggtaaaaata ttttataaaa tactgtactt ttcttcattt
    13321 tcttcctctc ctccttttaa aataaaaggc tttcattttt ctgtaaaatt gatactcatc
    13381 taattaaatg agcattcccc gctttcaaga atagcataat taaaaataaa aatccaagta
    13441 atttttaacg tgtgcaatct tttcatatca aagatctatt gacttatcgt agttatcgta
    13501 atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa aatgatggaa tggtaacgat
    13561 caaagatgtc gctaaagcag ctggcgtttc tcatacgaca gtttctcgtg ctttgaataa
    13621 cagcagttta ataaaaaatg aaacaaaaca aaaaatacga caactagcta aggaaatgaa
    13681 ttattcgcca aactatagtg caaaaggttt agtgaatcaa aaaaaatatc taatcggact
    13741 atttttctca agtttgcata aaggaacttc atcaaatttc ctagcagatt ccattgcagg
    13801 catccattca gtattagaca caaattacag tttatctgtt gaaagtatcg atgttgttga
    13861 tttaacagaa atcaacctgc aacgctacga tggactgatc gttatgagtc aaagtgatga
    13921 tgatcagcct ttcatcgact atctcaaaga aaaagaattt cctttcgttg ttgtcaatcg
    13981 tcacatcaat gatccagcgg ttatcaacgt cactgccgat gatgcggccg gtgttatgca
    14041 ggcagtagat tacggtattg gatgcggaca tacgaagatc gcttatattg gcggaaagaa
    14101 aaattttcgt tcgacaaaag aacgggaaaa aggggtattg aaaagtctaa aaaaagcgag
    14161 catccctgtc aatccagcct attttttaac aggagattac agcttagaaa gcggcctcca
    14221 aaacatggaa tacctattat cactttctga cctgcctact ctggtttttt gcggcaacga
    14281 cgacatagcg attggtgcat tgagagctgt acatttagct ggcctccgtg ttccccagaa
    14341 catttctctg atcgggttcg atgattctcc agtcgtctcc tatctcaatc cgccgcttac
    14401 caccgtacgt aagcctttga aagaaatgtg tcaagaagga acaaagcttt tactagattt
    14461 gatcgaaggc aggacgatcg aacaatctaa aattgagctc tccaccttgt tgatccttcg
    14521 tgagtcgatc aattacttgt aaactacaag taattttata taaaaatgtt aacgtgtgca
    14581 taacacagaa aggaagttta tatgaaaaaa ctgacaaaag aattagtgaa aaaaaacgaa
    14641 gcacctgtaa aagttcttca gtttggtgaa ggcaatttta tgcgtggatt tattgattgg
    14701 cagattcagc agctgaataa ccaacaattg tttaaaggta atgttgcaat cattcaacct
    14761 ttagaacaag gtcttggaaa tatgatgaac cagcaagata atttatatac ggtcatttta
    14821 caaggactca gcaatggcga aatcgttgat acttccgaga ttatcaccgt agtagaacaa
    14881 atgatcaatc cttatgatga atgggatgct tatctatctc tagcagaaaa tgacgacttg
    14941 gcctatatcg tctccaatac aacagaggca ggaatccaat tcgtggagac tgataattta
    15001 acagatactc ccccacagag cctcccgggc aaactgacag cctttttata ccgacgtttc
    15061 aaattaaaca aagctggctt taccatcatt ccttgcgaac tgattgacag aaacggcgaa
    15121 cgtctaaaag aaatcatttt acgctacgca gaaaaatggg atttagaacc tgcttttgtt
    15181 gagtggttag aaaaagaaaa tgttttctgt tgtagtttag ttgatcgcat cgtcccaggt
    15241 tatcctcgcg aagatgcagg tgttttcaat caacggcata actatgaaga tcaattgatg
    15301 gtcaaagcag aaccattcat gctttgggtg atcgaagggc ctaaagaatt ggagaatcaa
    15361 ctaccattca aacaggctgg cttgaatgtc atcatcacag atgatatgac tccttatcgt
    15421 gaacgtaagg tgcatttgct taatggaccg catacagcga tggtccctct tgctttgttg
    15481 gcggaattgg aaactgtaga agaagtaatg aaagatcctt tattctccgc atatgttgat
    15541 cagttattca atctagaatt gattcctatg ctttctttac ctaaagatga attagccatt
    15601 tatgctgacc aaatcaaaga acgtttcttg aatccttttg cccatcacaa attagaagcg
    15661 atttcattaa atagtgtttc aaagttcagc acccgtctgc ttccagtgtt caagaagtat
    15721 atcgaagaac aaaatcaggt accaccgtta atcactgttt cactagctgc gctacttctt
    15781 atgtatcgag gcgatcaagt aaagccacat gatgatgaaa agacgataag cgaatttact
    15841 gatgcttggt ctgataatgg aacagctatt cctcgattat tgcaaaacgc tgcgctttgg
    15901 ggagaagatt tgtctcaaat accgaatgtg acagatacag ttcaagaaat agctgcaaag
    15961 atcgatcaaa aaggtgtccg gtcagtatta caagaaatga aaggaacgat ggtccatgct
    16021 tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac agtgtggtcg tcgcattaac
    16081 ccctattcca aaacaaacac aactgacgat tgatcagcaa acgatcacca ctcttgaaga
    16141 tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa gctggagaca acgtgatcaa
    16201 atatggctat ccaatcggtc atgtgacgac agacgtaact gccggtcaat ggctgcacac
    16261 tcataatgta aaaaccaatc tttctggtga attggattat cgatatgaaa aagatgtcca
    16321 tccaagtcat tacctttttg agaaccgaac atttcaaggt tacttgcgaa aaaatggaaa
    16381 agtagggatt cgtaatgatt tgtttttagt acctaccgtt gggtgtgtga atggcattgc
    16441 tgaactgatc gtcaaacagt tcaaggaaaa acatccagat ctgggacagt ttgaccatat
    16501 tacgatttta aagcatcctt atggctgttc gcagcttgga aaagatcatc aaaatactcg
    16561 agaaatttta gcggacgcag taaatcatcc caatgccgga ggagttttag tatttggatt
    16621 aggttgtgag aacaatacag ttcctgaatt caaaaaaatt ttaggtgaat atgatgaaga
    16681 acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag atcgaacaag gtgtggctct
    16741 attagaagag cttttaacag cggcggaaaa tgaccagcgc atatcagtcc cgctctctaa
    16801 gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta tcgggaataa ctgctaatcc
    16861 tttacttggt gcattttctg actacttgat tgctcaagga ggcagcacga tcttaacaga
    16921 agtgccagaa atgtttggcg cagaacaagt attgatggca cgagcggaaa atgaagaagt
    16981 ttttgactca atcgttgact tgatcaatga cttcaaacag tatttcttgt cttacgggga
    17041 accagtttac gaaaacccat ctcctggtaa taaagcaggt gggatcacta cattagaaga
    17101 taagtcttta ggctgcacgc aaaaatcagg aagctctcct gtcgttgatg tgctacaata
    17161 tggcgaaaaa atcagaaaac cagggcttag tttattgcaa gctcccggga atgatcttgt
    17221 agctgcctct gctcttgctt catccgattg ccagttggtc ttgtttacca caggaagagg
    17281 gactccgttt ggctcatatg tcccaacggt caaggtatct acgaatacaa ctctttttga
    17341 acgcaaaggt cattggatgg attttaatgc aggcgtctta ttagaacaac caatggaagt
    17401 aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct agtggagaag aaacgaaaaa
    17461 tgaacaaaat gacgttcgag aaattgccat ttttaaaaac ggagtaacac tttgattttc
    17521 ataaaacagt ataaaaagaa agaaggaaga tttatgtttt tatctgatga ttttttatta
    17581 aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa aaaacatgcc cattattgat
    17641 tatcactgtc acttagatcc aaaagaaatt tacgaaaaca acaatttcag caatctgaca
    17701 gaagcttggt taggaggcga tcattacaaa tggcgcttga tgcgagcttg cggcgtgcct
    17761 gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat ttttagcttg gtgtcaaacc
    17821 gtaccaaaaa ttatcggaaa tccgctttac agttggtcac atcttgaatt gaagcgtttc
    17881 ttcggtattg acttgttgat aaatgaagaa aatgcagaga aaatttggac acaagcaaat
    17941 gaaattttgg cagctcctga ttttcgccgt cgggaaatcg caaaaaaatc gaatgtagaa
    18001 gtgatttgta caacagatga tcccgcagac gacttgcact atcatcagtt gcttgctcat
    18061 gaagaaccag atttgaaggt tctaccttca ttccgtccag ataaagcact caatattgaa
    18121 aaagaaggat ttaatgcatg ggtcacacta ctggaagaag cagctgacca aacgatcacc
    18181 acctatcaag aactgataga agtcttaggg cagcggatag aatacttcca tcaacatgga
    18241 tgtcgtgttt ccgaccacgc tttagacatc ttacgctata aagaagcgga tgaaacagta
    18301 ttagaagaga tttttcaaaa agcacgagct aacaaactat tatcttcaga tgaaatcgat
    18361 gcttatcgta cagaaacact gatacatttg acccacttct atcattctca taattggacg
    18421 atgcagctgc acatccatgc ttatcgtaac tgtaatacac aaatgttgga aaaattaggg
    18481 cccgataccg gctatgatgg aatgaatgat ctttctttga ctattcctct gcaaaaatta
    18541 ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga tcctttattc attgaatccg
    18601 aatgactatc ctgcgatttt agctttgatg ggctgtttcc aaaaagaaca gaatggaaaa
    18661 ctgcagttag gttctggatg gtggtacaac gatactcgag ctggtatgcg cgatcaattg
    18721 acacaatttg ctgatggtag tgctttgggg aattttgtcg gcatgctgac agattcaaga
    18781 agtttcctgt cctacactag acatgaatat ttccgtcgtg ttctttgtga atttttaggc
    18841 gaaatggtca tgcgaggaga agcacctgaa gatgaacaac ttttaggaag tctagttcaa
    18901 gcgattagtt atacaaatgc taaaaattac tttggctttt ttgcctaatt gattaatagt
    18961 aacagtaacg aatcaaaaac aggatagtga aataacggaa tccgtttatc cacgttttag
    19021 agaagaagac ggaccaaatt tgctccgtct tcttctcttg ctgttctgct tcattgccta
    19081 taaaccttta tgaaagaaga gataacatgc ctactatcca tattgccggt gattctactg
    19141 cggcagaaaa agatgaaaac aagcgtcccg agacaggttg gggagaaaaa atctctgctt
    19201 atttttcacc cgagatccag atcaataacc aagcaaagaa tggacgcagc accaaatcat
    19261 ttatccagga aggaaggtta gcccgtctag cttcacagtt tcagccgaat gatttcttat
    19321 ttgtacaatt tggacataac gaccagaaaa tccaagaaga aagaggtacg cttcccttcg
    19381 gtagctattt aaaaaatctg cagatatttt ttgaatgcgc aaaaaaagcg aacgtccaaa
    19441 tggtcttact gacatctgtg acgcgacgag attacttaga aaacggaaca ttgaatccag
    19501 atatacttgg agactatcca aaagcgatgc gtgcatttgc tgagaaacac catattcctc
    19561 ttttagatgt gttctcaaga tcacaagaat tgttccagac cttctcaaaa gaagagacaa
    19621 aaaaattcta cctgcatctt atacctgata cttgtaaaaa ttacccagaa ggactaaaag
    19681 acaataccca ctttagtcca gaaggcgctg ataaagttgc ccgaataatt gttgaattga
    19741 taaaagaatc ccgtctgcct cttgctaatt atttacaaaa aggagtttga gatgtttcca
    19801 ttaaatttag gcatccgtgc ccacgattta gatgttcgaa atcgtgaaga aatcgttcaa
    19861 aaagtatcac agcttcactt gtcccatatc caatttgcac cacaaaaagc atttcctgaa
    19921 caacttaaat taactgacat gacacttggg agtgctgcct attttggtga atacttccaa
    19981 aaaaacaata tcgaactttc cattttagga tgctatatca acctatccag caaaaatcaa
    20041 gaagttcgtg cacaagcatt ggcaactttc aaacagcaaa ttgctctgtg ctctgcctat
    20101 caagccaaga tgattgctac ggaaaccggt agtgtctctg tcggttacac aaaagaaaat
    20161 tttactgaag aagcttatca gatcgccaga aattctatca ttgaattagt cgctcatgca
    20221 gaaaattttg gcgtaactgt tgctgttgaa gcagggatca atcatccaat ctataactat
    20281 cagttagcta aacgtttaat cgatgaagta gcttcaccaa atttaaaaat cattttagat
    20341 tgtgcgaatt tgatgcataa agaaaatcat gctgatcagg aaaaagtgat ccgaggtgcc
    20401 ttggacaatt tacatggtta tattacagcg cttcatctca aagactatat catggaaaat
    20461 ggaaagatcc gcatggtacc tgttggcaag ggctgtttag atttccgccc ggtactgcat
    20521 tatatcaaat atgagcgtcc tctaatgtac gccactcttg aagctactcc tgagatttat
    20581 gttcctgaag caatagaaca cttgcagttt atttaccaag attgttaagg agaagattat
    20641 ggaggaacga atacagaaaa gttatcaaaa atatcatgca aatgcttttc agacattgct
    20701 gcatctttat cagggaaatt attggcgttt tgccatgtca ggactctttt ttattatcaa
    20761 acatcttcca tcttggttaa tgccgatcgc tattgccaat gtaatcaatg cagcttcatc
    20821 gaaagattct tctcagttgc aggccattta tttcaacgct tttttgatgg cttgtctgat
    20881 tggtcaaaat atattgacga attattttca tgtaaaatac catagcactt cgatccgtca
    20941 agtagaagct ggactaagag cgacccttat caagaaaatc caagaactat ctattcctta
    21001 tcaaaaagaa ttgcaatctg gacgaatcca gtcaaagatc atccgtgatg tagaagctat
    21061 ccaaacgttg tcctctcaag tatttgtcag cggattaaat atcgctgtca atttattcgt
    21121 agctttaggt atcacgcttt ataaaagtcc tgttgttttc atgtttttct tattgactgt
    21181 tcctctcgcc gcattcgtta ctctcttttt ccgtaaaagg atcagacagt ccaactcaga
    21241 cttcaggcaa gaaatggaag agacttctgc aaaagtgatc gaagtgatcg aattagcacc
    21301 gattgctcga gctcatgggt tagaaaaaca cgaaacaact cgcttaaata agcattttac
    21361 tcaaatctat gaaaaaggat tccacttaga tatcctgcaa tctgttttcg gatcagtcag
    21421 ctgggctagt tttcagatat ttcagctgat ttgtttgatt ttcactggga tattagcttt
    21481 acaagggcgc atccaacccg gcgatgtcgt gatgtatcag acgtatttca cgacgatcgt
    21541 caattccgtt tctggaatga tcacgctgat tcctacgatt tcaaaaggca tggaatctat
    21601 cacttctatt ggcgaactgt tgattgctgg cgatgtagaa acttatcacg gtaagaaaaa
    21661 aatccaacat ttggatggaa attttcgttt tgaggatgtt tcttttcttt atccggactc
    21721 actcaaaccg attatcgatc atttttctct agaggtgaaa gctggagaaa caattgcttt
    21781 tgttgggcca tctggctctg gaaaatctac actccttaac ttattgattg gatttatcca
    21841 gccgacttca ggaaaaattt ttctagatga tcagcctttg gatgaactgg atatgcgaag
    21901 tgtgcgaaat tatttagccg ttgttcctca aaccactttg ttgttctccg cttcgatcaa
    21961 agagaacatc acttatggct tgaaaaacgt atcaaaagaa cgtttagatg aagtcatcga
    22021 agctgcgcaa ctttcttcac tgattgacca gcttcctgat ggtctggata ccttagtagg
    22081 cgaacatggc aacaagctgt ctggtggcca aaagcaacgt atctccattg cacgagcgtt
    22141 gatacgtcag ccaaaaatca ttttgctaga tgaagcaacc tcagctttag ataaccagtc
    22201 agagaaaaaa atccagcaag ccctgaatta cctgacagaa acacccacta cttttattgt
    22261 ggctcatcgg ctttcaacga tcaaagaggc agataaaatc gttgtgatcg atgaaggaaa
    22321 aatcgttgaa ataggtactt acgaaaaact gttagctcaa aaaggctatt tctaccgctt
    22381 gaaaaatgaa tgacaaaaaa taggagctat gacaagactt ttgtcgtagc tcctatttct
    22441 gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatagtc atcaaacatg
    22501 agaagctgtt ttttgaaatt gttccttgta actcagagtt aaacggtagg ctcacgacct
    22561 cttggtaaat ggtgttcaag agacagccta tcggcaataa gaagcaattt tgtcatcgct
    22621 caaggccgaa cgtctctttc acaccctctg ttgtttcata aaatagtttt attcgctgta
    22681 aaggtcgcga actggtgtca tgatgatacg gttcaagtca ttgatgattg tactgaagcc
    22741 ttgctctttt tgcatcaagt cattgattac ttcttctgat tggattttca cagccatttc
    22801 ttgtgctttc attgcatctt cgtcgctgaa ctcttgtcca ctcatttgtt tttcttgtaa
    22861 ttcgatttgc atcgcttgga agtctttgaa cagtttgtgt gcttcttcgt ttgcttgtac
    22921 tttctcgtac gcttctttca acgctttgaa ttctggtaat tcacggatct cacgttcgat
    22981 ttgattcgcg ctatcgtaga tgtttgacat gttatgccct cttttctttt agtagttact
    23041 ctctattgta acatggggtt attcgttttc aaaggggcta ctctccttga tatttccgcc
    23101 agaggtcttt agccgcttca ccaattccct gaacgccttc tttcaccata tcgccgaagt
    23161 ttttcagccc ttcttttgct tgttcaccat attgattcaa gttatcttgc caatttccag
    23221 actctccatt agaatttcct gtgtttccaa cttgatctgc agctaccact tgtcctcctg
    23281 tggcataggc atctgctaca ctaaatcctg tttttgaagt atacggaagg atgccttctg
    23341 ccgctgattt gaagactttc ccgacaacat ttccacttgt cccttccaaa tagtgcagct
    23401 tgctggattc ttcgtaaccc atccatgtcg aaatcactac gtctggcgtg taaccgatca
    23461 tccattggtc atttgctttt gtggcatcga aattagtttc tgtggttcct gttttccctg
    23521 ctatcgtata acctgcagga ttcgcagcag ccgctgttcc atttgaaaat gtaccaagaa
    23581 gcatgctggt catttcatcg gctgtttcct ttgatatgac ctgttttggt tttttgtttg
    23641 tgttatcgac gatcaccgct ccagtcgaat caacgatttt cgtgataaga tgcggcgtat
    23701 acatctgacc atcattggca aatgcactgt aagcacccgc catgatcaat ggagatactc
    23761 ctttttccag tccaccaagg gctaagccat aatacttatc agattttgct aaaggcaggc
    23821 cgaattcttc tgctttatca tatccttttt gtaaaccgat ctcatgcaaa agccatacag
    23881 taggcaaatt caaactttgt gcaacagctt gatacatcgg tacttctcct tggtatgtcc
    23941 cgtcaaaatt atgcgcatcg taataacttt gcggttcgtc tttcaagata ctggaaggct
    24001 tgtatccagc ttccagtgct ggtgtatata cgcttaatgg tttgatcgtt gatcctggtg
    24061 aacgacgcat ctgtgtagca aagctgaatc ctctaaagac atgatctcct cttcgtccta
    24121 ctagtgcttg tacaccacct gtattcggat ctaaagctac tgaagcagat tggaccatgg
    24181 caccatctga agcatttgct gggaataatg cactgttttg ataagtagct tctaattgct
    24241 tttgataatc ctgatctaaa gctgtataga ttttatagcc tttattcatg acatcttctt
    24301 catcaagacc atactcgtta actgcttcat cgatcactgc atcaaaataa taaggataac
    24361 gataaccaga atctgaatta tcgtatgtgt cattaagata cgcgctgata tctgtctggc
    24421 tttctgcttg ttcttgttct tttgtgattt ttccgttttc ttccattaca cttagtactg
    24481 tgtttttccg atcattcgca ttttttgggt gatcgatcgg attataaata ccaggacctt
    24541 tcagcattcc tgccaaagta gcagcctcag acagcgtaac attcgttgca tctactccaa
    24601 aatactttct ggaagcgtct tggacacccc ataccccgtt gccaaaatag gcattgttaa
    24661 gatacatggt caagatttct tctttgctgt acttcttttc gatctcgatt gccaaaaata
    24721 attccttcgc ttttcgatca aacgtttggt ctaatgtcag atacgcattt ttggcaagct
    24781 gctgagtgat cgtacttcct ccgccccctg atgttccgcc agacaagacc attcggactg
    24841 ctgcacgggc aatccctttg atatcaaacc cgtgatgctc gtaaaaattc ctatcttctg
    24901 tcgagattac tgcatcttgg atatagggag aaatcgcgtt taattctaca tatgttcctt
    24961 tttgggcgta cagtgtgcca gcttcttcgt ttttcttatc ataaatgact gtcgattggc
    25021 ttaatcctga ttttaacgtt tcgacatttg cttgttttgc taaaataaag aggtagatac
    25081 ttgtcactaa gacacagatc aaaccaatca gcaaaatcaa tttgttcaca tgatattttt
    25141 tccagatgcg ctttcgccat cgatggaatt gtccgagata tggtctgatc caacgccaaa
    25201 aactgatcaa agcagctttt atcttttcta aaatgctttg aaaatccatt tgcttcttct
    25261 cctgtctatt tgattcaatc gtcttatttt accatagtga aagagtgttt tcttcctgct
    25321 taagttgtag ttcaatatgt ttttttacgt tagtgcaagt agagtaacat aagaatgaac
    25381 agaaaaatgt aagttttttc ttaaatatgt tttaaaaatg gtacaattag tctgcgattg
    25441 cactgaaagg agtacacttg tgaaaatcac gatcacttta cctatgaacc atcctgaaac
    25501 aacaattcgc gacttgcttg aaaaggaatg gttagttcct agaaaggttc gacacttttt
    25561 gcgtatacgt aaaaacgttt ggatcaatca acaacctgct ttgttccatc atgaagtaaa
    25621 gggcaatgac cagatcacat tgttattcga agaaacagat tataattatc aagaagttca
    25681 attaggagat gcgaagcaca tccgtgtttt atatgaagat gagcatttga ttatagtgga
    25741 taagcctgca ggaatgaaga ctcatccaaa tgaaccgaca gaaaacagta ccttactcaa
    25801 tcatttagct gcttatctcg acactaaaca gcaacgacct tacgttgtcc accgcttgga
    25861 caaagaaaca agcggtgccg ttttatttgc aaaaaatcca tttgtcttgc cgatacttgg
    25921 aagaatgttg gaacaaaaaa tgatctatcg gagatatcaa gccatcgtat ggggagaaat
    25981 tgccaaagat atgaccatca ccaataaaat tggacgagat cgccatgatc ggagaaaaag
    26041 ggtcgtagat gataaaaatg gtcagagcgc cttgacacat gtcactgtca acaaagtact
    26101 ttctgaacaa acacaagtct attgtgtttt ggaaaccgga cgtacacacc agatcagagt
    26161 tcatttagct cataagggac atccgatcgt tggagaccct ctctaccaaa atagaacagc
    26221 tgaacgactg atgctccatg cattagaact gcacatgatc catccattta ctcaagaaaa
    26281 aatagtcggt aaagctttac ctggattatg ggaatagaaa taaaaaagaa cagagtctta
    26341 tcagcagata atgataagac tctgttcttt ttgtgtaatt tgtctgctta cttttgatgt
    26401 tcgtgttctg ctaacagcaa actaccgata atcccacttt cattcggcag tccccaacga
    26461 acgatatatt cacttacttg aggtgtttcc atataaccac ccatcaattc aacaaattgc
    26521 tgacggattt tttgaaggag atgatcctga ttcattaccc caccgcctaa aataatcttt
    26581 tcaggtgcta acgtcaacgt atagttgatc agtgcttgag caatataata agcctgaata
    26641 gcccaaacag gatgatcttg cggcagatct tgccctttga tccctgtgcg tgcctcaata
    26701 gacggtcctg ctgctaatcc ttctaaacaa tctttatgat aaggacaagt accttcataa
    26761 tggtcatctg gatggcgttt cacccaaata tgtcccattt caggatgtgc gatccctgaa
    26821 aaaatttctt cattgatcac gacacctgca ccgattcctg ttcccacggt aaggtaaata
    26881 caactatttt ttccttgtgc tgctccttgc ttcaattccc catatgcagc agcattgaca
    26941 tccgttgtcc aagcagcagg aatattgtag cgttccttga ttgtgccaag aaaatcgaat
    27001 tgtgcccacc cttttttggg agttgccaaa acgtatccgt aattatcttt tgttttgtcg
    27061 ataccaatcg ggccgaatga gccaatcccg attcccttca attcataacg atcaaaaaat
    27121 tcaaatacct gatccaatgt ttcttctggt gttgtggtag ggatacttaa ttt
//
LOCUS       DAZKOK010000022        26840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000022 DAZKOK010000000
VERSION     DAZKOK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.022"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG90W_000891"
     CDS             complement(104..1336)
                     /locus_tag="ACG90W_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0477270.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG90W_000892"
     CDS             1591..2160
                     /locus_tag="ACG90W_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0477271.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG90W_000893"
     CDS             complement(2338..2778)
                     /locus_tag="ACG90W_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0477272.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG90W_000894"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG90W_000894"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0477273.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG90W_000895"
     CDS             3732..4655
                     /locus_tag="ACG90W_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0477274.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG90W_000896"
     CDS             4731..5870
                     /locus_tag="ACG90W_000896"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0477275.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASNIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG90W_000897"
     CDS             5863..6663
                     /locus_tag="ACG90W_000897"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0477276.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG90W_000898"
     CDS             6663..7490
                     /locus_tag="ACG90W_000898"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0477277.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG90W_000899"
     CDS             7495..8202
                     /locus_tag="ACG90W_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0477278.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG90W_000900"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG90W_000900"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0477279.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG90W_000901"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG90W_000901"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0477280.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG90W_000902"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG90W_000902"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0477281.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG90W_000903"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG90W_000903"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0477282.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG90W_000904"
     CDS             11787..13820
                     /locus_tag="ACG90W_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477283.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG90W_000905"
     CDS             13864..15144
                     /locus_tag="ACG90W_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0477284.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG90W_000906"
     CDS             15354..16160
                     /locus_tag="ACG90W_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477285.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG90W_000907"
     CDS             16172..17392
                     /locus_tag="ACG90W_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477286.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG90W_000908"
     CDS             17382..18968
                     /locus_tag="ACG90W_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0477287.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG90W_000909"
     CDS             19007..21145
                     /locus_tag="ACG90W_000909"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0477288.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG90W_000910"
     CDS             complement(21513..22496)
                     /locus_tag="ACG90W_000910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0477289.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG90W_000911"
     CDS             22874..24265
                     /locus_tag="ACG90W_000911"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0477290.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG90W_000912"
     CDS             24281..25321
                     /locus_tag="ACG90W_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0477291.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..26425
                     /locus_tag="ACG90W_000913"
     CDS             25340..26425
                     /locus_tag="ACG90W_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0477292.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26458..>26840
                     /locus_tag="ACG90W_000914"
     CDS             26458..>26840
                     /locus_tag="ACG90W_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0477293.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQI"
BASE COUNT         8613 a         4454 c         5449 g         8324 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtaatatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    25561 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    25621 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    25681 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    25741 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    25801 tgaaagtctg tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct
    25861 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    25921 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    25981 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    26041 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    26101 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    26161 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    26221 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    26281 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    26341 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    26401 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    26461 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    26521 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    26581 tttattgaag catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt
    26641 aaaatttatt ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca
    26701 ttacaaaatg agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa
    26761 gataatgagt atatttataa ttattttaaa aagaatagtt cagtggagtt tattcgtgga
    26821 atagatctag atcaaattaa
//
LOCUS       DAZKOK010000023        26576 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000023 DAZKOK010000000
VERSION     DAZKOK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.023"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG90W_000915"
     CDS             <1..1152
                     /locus_tag="ACG90W_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0477294.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG90W_000916"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG90W_000916"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0477295.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG90W_000917"
     CDS             2728..3003
                     /locus_tag="ACG90W_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0477296.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG90W_000918"
     CDS             3009..3776
                     /locus_tag="ACG90W_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0477297.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG90W_000919"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG90W_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0477298.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG90W_000920"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG90W_000920"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0477299.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG90W_000921"
     CDS             7920..8222
                     /locus_tag="ACG90W_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0477300.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG90W_000922"
     CDS             8225..9388
                     /locus_tag="ACG90W_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0477301.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG90W_000923"
     CDS             9414..12842
                     /locus_tag="ACG90W_000923"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0477302.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG90W_000924"
     CDS             12863..13987
                     /locus_tag="ACG90W_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0477303.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG90W_000925"
     CDS             13968..14414
                     /locus_tag="ACG90W_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0477304.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG90W_000926"
     CDS             14398..14736
                     /locus_tag="ACG90W_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0477305.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG90W_000927"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG90W_000927"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0477306.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG90W_000928"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG90W_000928"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0477307.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG90W_000929"
     CDS             15893..16936
                     /locus_tag="ACG90W_000929"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0477308.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG90W_000930"
     CDS             17095..17823
                     /locus_tag="ACG90W_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0477309.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG90W_000931"
     CDS             17939..18184
                     /locus_tag="ACG90W_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0477310.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG90W_000932"
     CDS             18181..18399
                     /locus_tag="ACG90W_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0477311.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG90W_000933"
     CDS             18535..19074
                     /locus_tag="ACG90W_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0477312.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG90W_000934"
     CDS             19067..21340
                     /locus_tag="ACG90W_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0477313.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG90W_000935"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG90W_000935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0477314.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG90W_000936"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG90W_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0477315.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG90W_000937"
     CDS             complement(23167..23436)
                     /locus_tag="ACG90W_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477316.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG90W_000938"
     CDS             complement(23698..23826)
                     /locus_tag="ACG90W_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477317.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG90W_000939"
     CDS             complement(23906..24361)
                     /locus_tag="ACG90W_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0477318.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG90W_000940"
     CDS             complement(24499..26427)
                     /locus_tag="ACG90W_000940"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0477319.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8844 a         4606 c         5612 g         7514 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaa
//
LOCUS       DAZKOK010000024        25841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000024 DAZKOK010000000
VERSION     DAZKOK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.024"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..2178
                     /locus_tag="ACG90W_000941"
     CDS             118..2178
                     /locus_tag="ACG90W_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0477320.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2261..2830
                     /locus_tag="ACG90W_000942"
     CDS             2261..2830
                     /locus_tag="ACG90W_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477321.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3005..3811)
                     /locus_tag="ACG90W_000943"
     CDS             complement(3005..3811)
                     /locus_tag="ACG90W_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477322.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3949..5463
                     /locus_tag="ACG90W_000944"
     CDS             3949..5463
                     /locus_tag="ACG90W_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0477323.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5866..6849)
                     /locus_tag="ACG90W_000945"
     CDS             complement(5866..6849)
                     /locus_tag="ACG90W_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0477324.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6854..7501)
                     /locus_tag="ACG90W_000946"
     CDS             complement(6854..7501)
                     /locus_tag="ACG90W_000946"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0477325.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7586..8218)
                     /locus_tag="ACG90W_000947"
     CDS             complement(7586..8218)
                     /locus_tag="ACG90W_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0477326.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8234..8785)
                     /gene="coaC"
                     /locus_tag="ACG90W_000948"
     CDS             complement(8234..8785)
                     /gene="coaC"
                     /locus_tag="ACG90W_000948"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0477327.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8782..9519)
                     /locus_tag="ACG90W_000949"
     CDS             complement(8782..9519)
                     /locus_tag="ACG90W_000949"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0477328.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9516..10505)
                     /locus_tag="ACG90W_000950"
     CDS             complement(9516..10505)
                     /locus_tag="ACG90W_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0477329.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10763..11305
                     /locus_tag="ACG90W_000951"
     CDS             10763..11305
                     /locus_tag="ACG90W_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0477330.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11322..12407
                     /locus_tag="ACG90W_000952"
     CDS             11322..12407
                     /locus_tag="ACG90W_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477331.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12419..13225
                     /locus_tag="ACG90W_000953"
     CDS             12419..13225
                     /locus_tag="ACG90W_000953"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477332.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13226..14053
                     /locus_tag="ACG90W_000954"
     CDS             13226..14053
                     /locus_tag="ACG90W_000954"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477333.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14050..15123
                     /locus_tag="ACG90W_000955"
     CDS             14050..15123
                     /locus_tag="ACG90W_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0477334.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15551..18247
                     /locus_tag="ACG90W_000956"
     CDS             15551..18247
                     /locus_tag="ACG90W_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0477335.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18312..18812
                     /locus_tag="ACG90W_000957"
     CDS             18312..18812
                     /locus_tag="ACG90W_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0477336.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18824..19318
                     /locus_tag="ACG90W_000958"
     CDS             18824..19318
                     /locus_tag="ACG90W_000958"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0477337.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19339..20148
                     /locus_tag="ACG90W_000959"
     CDS             19339..20148
                     /locus_tag="ACG90W_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0477338.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20145..20966
                     /locus_tag="ACG90W_000960"
     CDS             20145..20966
                     /locus_tag="ACG90W_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0477339.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21017..23116
                     /locus_tag="ACG90W_000961"
     CDS             21017..23116
                     /locus_tag="ACG90W_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0477340.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23113..24231
                     /locus_tag="ACG90W_000962"
     CDS             23113..24231
                     /locus_tag="ACG90W_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0477341.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24270..25148
                     /locus_tag="ACG90W_000963"
     CDS             24270..25148
                     /locus_tag="ACG90W_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0477342.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25294..25491
                     /locus_tag="ACG90W_000964"
     CDS             25294..25491
                     /locus_tag="ACG90W_000964"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0477343.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            25475..25705
                     /locus_tag="ACG90W_000965"
     CDS             25475..25705
                     /locus_tag="ACG90W_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477344.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT         8173 a         4480 c         5446 g         7742 t
ORIGIN      
        1 atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat
       61 catcttatga aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg
      121 tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg
      181 tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct
      241 gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt
      301 acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca
      361 aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg
      421 ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt
      481 gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag
      541 atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt
      601 tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca
      661 gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa
      721 ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga
      781 acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct
      841 gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga
      901 acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact
      961 tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa
     1021 tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct
     1081 gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt
     1141 caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat
     1201 ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa
     1261 caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa
     1321 agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa
     1381 tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt
     1441 catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca
     1501 acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta
     1561 ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat
     1621 catgacccgt attcactcca agaactccat gctcagtcac tattacatgt tttaccatta
     1681 aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa
     1741 caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag
     1801 ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat
     1861 ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa
     1921 tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag
     1981 gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa
     2041 gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta
     2101 ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac
     2161 gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca
     2221 tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc
     2281 ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc
     2341 ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga
     2401 gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt
     2461 tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga
     2521 aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt
     2581 tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc
     2641 cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca
     2701 gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga
     2761 aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt
     2821 agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca
     2881 ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca tgccatacat
     2941 tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca
     3001 tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat
     3061 cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa
     3121 ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg atgattgttt
     3181 tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat
     3241 gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat
     3301 ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa
     3361 caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca
     3421 tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa
     3481 atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta
     3541 actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg
     3601 gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca
     3661 gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa
     3721 cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac
     3781 tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat
     3841 tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat
     3901 tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta
     3961 gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag
     4021 aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct
     4081 tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt
     4141 ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca
     4201 gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg
     4261 aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc
     4321 tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt
     4381 gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac
     4441 ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct
     4501 attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt
     4561 tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact
     4621 gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca
     4681 ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat
     4741 attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt
     4801 ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg
     4861 gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa
     4921 agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct
     4981 gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa
     5041 ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa
     5101 atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg
     5161 gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg
     5221 aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc
     5281 aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct
     5341 ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag
     5401 atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa
     5461 tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg
     5521 tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa
     5581 taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc
     5641 agatgcaagc ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac
     5701 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa
     5761 ataagctgaa attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg
     5821 gagttaaacg cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt
     5881 caaaatgatt tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc
     5941 gctgaattgg aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc
     6001 agctttttgc ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa
     6061 taatgtacca agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac
     6121 gatggtccca cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa
     6181 attcttttcc cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc
     6241 agtgattttt cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc
     6301 accaagttct ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc
     6361 gtgagaaagc tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg
     6421 gatcaagaca gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt
     6481 caaaaaaact tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat
     6541 tgcacgatcg gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta
     6601 tacttttgtc ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc
     6661 tacttcgact cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat
     6721 atctgttcga ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt
     6781 aggaacaggc acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat
     6841 ggcacgcatg gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc
     6901 taccttcaga aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa
     6961 gtacagcagc aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta
     7021 attgaatgga agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat
     7081 ctaagttagt tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca
     7141 tggactcagg ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag
     7201 agacatgcgc attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca
     7261 gaagattctt tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt
     7321 cggtcatttt ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt
     7381 ttactaatgt ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag
     7441 ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca
     7501 tttggatcat cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa
     7561 aatcagagat cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta
     7621 acaagacatt gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac
     7681 ttgtaaaaat aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt
     7741 tggcataatc ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca
     7801 atgaaccgga aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt
     7861 tgcgcagacc tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg
     7921 cgatcagcaa ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa
     7981 agacaaaaga caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg
     8041 taccgccaag aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa
     8101 tatgcatggt tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta
     8161 ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa
     8221 gtactccttt tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg
     8281 tctgaatatc agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg
     8341 gttcaatttc ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt
     8401 tttgatacat attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg
     8461 ccattgcgac tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt
     8521 ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga
     8581 ttacctgcgg atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt
     8641 gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg
     8701 cacctaattt gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag
     8761 taacgcctag taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa
     8821 tgcagcaatc tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata
     8881 gccgtggtgt gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt
     8941 gttatttatc agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa
     9001 tagtttgaaa ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt
     9061 tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt
     9121 ggtatctgtc tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt
     9181 gggtaggttt tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac
     9241 tgccatactg tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa
     9301 aaataagtcc agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt
     9361 cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc
     9421 agctaatgct tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat
     9481 tttttcagtc gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact
     9541 agtgttcttc cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc
     9601 agagtaatct cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat
     9661 aaagaaagac gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt
     9721 cgcagaataa aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa
     9781 gctactgcac cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact
     9841 gtgtcgatga catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat
     9901 tcatcaggtc gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct
     9961 tttctggaaa aggtaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta
    10021 ctgcctacac cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga
    10081 cggattaaag cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct
    10141 tgacgcagcg tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat
    10201 tggctataac cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct
    10261 tcttttgaaa aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga
    10321 gtcatcggat atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa
    10381 ttgacatcag aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc
    10441 agatcactga ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg
    10501 ttcattcgtg agacactcct tttaaaattt tcttttatca taccataaag cctactcttt
    10561 tattcttgtt cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac
    10621 tagaatttat tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc
    10681 tttttcaata aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga
    10741 aaacttcgat tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca
    10801 aaagaatttg acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc
    10861 ccagttggaa cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt
    10921 cttaggtgtg actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta
    10981 ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct
    11041 cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg
    11101 agaatataag acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga
    11161 attagagtta agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta
    11221 tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat
    11281 tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt
    11341 catttgatca agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg
    11401 agatcgaaca aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga
    11461 tcctacgtat tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga
    11521 aacggttgaa tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg
    11581 cactttttcc tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa
    11641 tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg
    11701 gatacgaaaa acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg
    11761 cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg
    11821 atttgaaact acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa
    11881 tcacgtttat ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt
    11941 ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc
    12001 caatcaatca tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga
    12061 tcgaagataa tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc
    12121 gtctaaatga accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag
    12181 ataaaggaaa actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa
    12241 tcatctgtta tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag
    12301 aaggaagcaa agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg
    12361 agtctgaaga agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat
    12421 gaaaacaatg cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat
    12481 cgcgcctgta ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct
    12541 gaataattat ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt
    12601 atggtatgca tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt
    12661 tttaacaaaa ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt
    12721 gaatctgctg cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa
    12781 tcatttcttt gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt
    12841 tctgttcgtg gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt
    12901 ggaagagatg aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga
    12961 gaccttccga cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc
    13021 agtatttatt ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt
    13081 gatcacttta ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg
    13141 ttctacaatc ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga
    13201 gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg
    13261 gatcgtcttt ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga
    13321 aggaggaacg atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga
    13381 agatacacgt ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt
    13441 gatcgccacg atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg
    13501 tgcacggaca gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat
    13561 cggtgctagt ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt
    13621 attgctttcc catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt
    13681 gcaggaaatg aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca
    13741 agtagtcaaa aatatcattt tgccttatct ttctccagga atcatagctg gatattttat
    13801 ggcatttacc tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt
    13861 tacgacgctc tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa
    13921 tgcgctaagt gcgttagttt tcttgttctc catgatattg gtcattggat actactttat
    13981 tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc
    14041 acggctgcga tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg
    14101 ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt
    14161 accatctata actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa
    14221 tatggataca aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc
    14281 cagcaaggtg ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg
    14341 atcaaagaaa aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt
    14401 gatccacgat ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt
    14461 tggggaactt tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat
    14521 tgggatgatt tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga
    14581 gaagttatgg ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca
    14641 cagctgacag aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg
    14701 gcagatgaga tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct
    14761 ggcgaagctg ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa
    14821 ggatccaatc tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt
    14881 gcctatgatt tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat
    14941 attggttatt ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg
    15001 gacgaacaat tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta
    15061 ggatcgaaat acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga
    15121 taatggcaaa actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc
    15181 ctttttctat ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg
    15241 tgttatttta ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta
    15301 aaatagggcc cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt
    15361 aattggaaac tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat
    15421 gaatccagtt caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta
    15481 aattggtaga taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc
    15541 aaagtaaaaa ttggaataca aatatagaat agacatattg cagccgatag tttattatgc
    15601 ttttagacac attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga
    15661 tgagctgata cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa
    15721 tctaggtatc tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa
    15781 ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt
    15841 agataatacg gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct
    15901 gagagaggtc attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt
    15961 attaataaat ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata
    16021 cgctatccat gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga
    16081 ttatgcggac aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt
    16141 tacgggggct gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt
    16201 tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga
    16261 tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt
    16321 tattttcgcc acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat
    16381 tcaaattagt gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct
    16441 gattcaatta ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag
    16501 agaagccttg tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa
    16561 tatcattaaa atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat
    16621 tgggaaaaat gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc
    16681 gattgctagt ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt
    16741 tgattatgat tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga
    16801 aaatttttct tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc
    16861 ttttttatca atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt
    16921 tttaggaaaa acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc
    16981 ctatgtcatt tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc
    17041 aaatctacct cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc
    17101 agaaaaggac gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga
    17161 tgaatccatc gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat
    17221 tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt
    17281 agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa
    17341 tacagatagt ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat
    17401 caagaacgat ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact
    17461 tgatattggc ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc
    17521 agacaagtat tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat
    17581 catttcctgt atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca
    17641 tttatcggat gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat
    17701 cgaccaaaat gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct
    17761 tccacgttcc ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc
    17821 agccaatttg gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat
    17881 tcaggagcta gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa
    17941 cccaacgatc gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca
    18001 tttcacttta aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga
    18061 acgtttattc ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga
    18121 tgaagatgaa ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa
    18181 ttttaaagtc aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca
    18241 aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca ctttttttaa
    18301 tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatataa
    18361 aagtgaacag aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc
    18421 acttagcttc aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta
    18481 cggtgattaa tgcttatgtg gatgagtgtg attttgaaca ggagttgaat gtatttgtaa
    18541 accaggtgaa cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca
    18601 atcagaagat taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct
    18661 ttaatctgcc aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata
    18721 ttcaggaact ggtaaataaa tgccaacagg aattaaagat gaccattctt gatgaggtac
    18781 atgtagtaaa tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg
    18841 aattgatgaa cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt
    18901 aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa agatgacatt
    18961 gaagatggct gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca
    19021 tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa
    19081 agatgcggta aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa
    19141 tgctgggaaa atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt
    19201 aacgacgtca gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt
    19261 tcagcctaca ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa
    19321 aaaggaagga gaaaaattat gtatgatgca tttgtagtcg ctttggccgt ttttattgga
    19381 gttgctggac atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt tgttgctccg
    19441 ttagcaggag cgttgatggg ggatttgcag acggggttga ttgtcggggc gtcactagaa
    19501 gccatcttta tgggtgtcgt caatatcgga atctctagta cggcggagcc ttctttggct
    19561 gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc gattattccc
    19621 attgtctttc cactagcagt attaggatta cagttaatga atatgatttt ttcttttgta
    19681 tgtggaccga tggctgctaa atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc
    19741 atcaatctgc acttctttat gtggtttgtc cactatgcgc tttatgctct tattccattc
    19801 tttgccgttt tatttggagc agatgttgtc aaaagcgtct tggataatat tccagaggtc
    19861 atcatgaacg gattaacagt cgctggaaat ctgctgccgg cagttgggat ggcgatgctg
    19921 ttacggatgc tttggagtaa aaatattgct gtgtggtttt tctttggggc cgttgttatg
    19981 gcgtatctcg agttaccatt gattgcaatt gccgcagttg gaacgattat tgcaattgtt
    20041 atagcccaaa gagatctaca actaaaaaat atatctgttc atgcagttca aagcgatgct
    20101 gctgtaaatg caacgcttga ccaagaagag gaggatttct ttcaatgaaa ttagctgatg
    20161 gaatttctaa agatgagaaa aaattggttc ggaagatgtt ttggcgctcg atgactatgt
    20221 atgcttcggt gaatccggtg acgatgggcg gcggtggttt cgcctattct atgatgccct
    20281 tcattaatca tttctacaaa agtgaagaag atcgagctgc ggcgctggaa cgtcatacac
    20341 agtatttttc gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg
    20401 agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg atcaaaacga
    20461 gtttgatggg accattggct ggaattggcg attcattgtt ctggggagtt tggcgtgtcg
    20521 ttaccgcggg actagcgatc aacttagcca atgccggaaa tattttggca ccaattatct
    20581 ttttagttat gttcaatata cctaattact tgatgcgata ctatggcggc gttttaggct
    20641 attcattagg ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt gaaattctga
    20701 caaaagctgc tagtatcttg ggtctgatca tggtaggtgc aatgacgatc agtatggtca
    20761 aattcaatac cgttttgagc ttttcaatga agggcgaaga actaatgaat gtccaagaaa
    20821 tcctcgatag tatttttaaa ggaattgtac cgttattggt aacattgagc tgtttcaaat
    20881 tactagataa aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt
    20941 tattgtcatt acttggaatc gcatagtagg aagctgcagc tactaggctg cagttttttt
    21001 agcaaggagg gaaaagatga ctattaatga agtaatttat tcacccaaac acccattttt
    21061 taatgctgag ttaaacgacg gaaatgtgac tacttcaaac gaatacagct actatcggaa
    21121 tattcctgat gtaaagggat tgaccttcga tgtaagtaat caaaagacat tagcaaacct
    21181 tgatgtttgc gggacgattc aaacgttgac cttttatcga gaaaatttac taactgagga
    21241 aaaaccaggt gtttgggtca ataaacagct ctctcaaacc aattcgttat ccttaaaggt
    21301 tgaagtaaac gggattgtct accatttgtc agaatcggat catcgtattg aagtcgatgt
    21361 gattcaagac tgtttgccta gatatattca tcattattcg acgtttaagg tcattgtagt
    21421 atcctttgct cccatactgg ctaataagcg tttaagtatg cttgttcaac aggtttacat
    21481 tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga
    21541 gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc
    21601 acaacaggaa gtggcaagct tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt
    21661 aatgactttt caggaatctg atatcgaaaa atggctgaag gaaacattga actattttgc
    21721 tcagctattt gggcagctgt caatgccaaa cgatagagtt gtccatctct atcgtcgtgc
    21781 actttatcaa agcttttcta gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa
    21841 ttggggcagc tatcctgcta ctaatcgaat ttggaataag gatatgtatt acgcatcatt
    21901 gccattcacc atgtttgaac cggagttatg tcaaaaaacg gttctatggt ttgatcaata
    21961 tggcgttaag tttcctggtt caaaatttac aggaggtatc aatcattctc tgagtaattc
    22021 gttatccagt gcgttactgg ctagtcttta ttttgaacat actggggata caaaattctt
    22081 tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt attgaggaca tattagctca
    22141 acgtcggtca ggggaaccaa tgctttttcc atctgtttgg ttatcagacg cctttgcact
    22201 tggaaagtat cataccggca gcaatctttg tctttggaag gcctgtattg gacttgcaga
    22261 aatttttgaa gccctagaac aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa
    22321 actaaaggaa tcaatttgta aacaaatgac tatcgatggt ccttttggtg agcaattttt
    22381 ggagggaatc ggtgatgagg agaaaacaac ctattcagtg aagcactatc aaaaaccgat
    22441 tcttgagcaa gggttaatct ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt
    22501 gatgcatgat ggagaagaat cagacactac gttgatgcct ttttatcaat ttttagataa
    22561 taaagatcat ttatatcaga acaccatggc cttttctgct agttctttca atccaaccta
    22621 tagcgaggaa atcaaaggca tcacttgggg attagaatcc ggcgccactt ttcctggctt
    22681 tatcactgtt ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc gattagaaga
    22741 actaatttgt ttggcagatt tagacggttc gtggtggtgg tggccgtatc gcttagaggc
    22801 tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg catcgggtct
    22861 ttttgtcagc ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag gagtattgca
    22921 aattgatcct gttgataatt taacgttttc ttggaagaat ttgaagttcg gtcaagcctt
    22981 catttcgatt gaatgtacgc aatccgagtt gagtatttca aatttgtcag aaaagccatt
    23041 gaaaataagt gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga ttcacattca
    23101 aaggagaaaa agatgagtat actaaaaaat attacccaat ttaaaaaaat ccagagtcga
    23161 gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag
    23221 ctaggaccaa gccgcaaagg tcgtggcgca attccgctga cttctggtga agaaactgtg
    23281 attgcggata ttactggaac gggaataatt cgtcatatgt ggatgacgat tcgtgatcga
    23341 acagaatttg gcagttttgt tttacgtgat ttaatcttaa gaatttattg ggatgggtcg
    23401 gatagccctg ctgttgaaag tcctttaggg gatttctttt gtaatgggtt tggagagcgt
    23461 tgcgaggtga actcgttgcc gatagtggtt aatcctacag ggggctttaa ttcctatttc
    23521 gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga atttacatcc gaaggatatg
    23581 tcctcatttt tttatacgat aaattacgct ttggtggacg aattggaaga aaatagccta
    23641 tattttcacg caaattggca tcgggaacgc attaccaaat tacaggagga ttataagtta
    23701 attgatcgat taaagggtca aggctattac gtgggtactt accttgcgct tacctgccta
    23761 gaacgttatt ggtggggcga aggcgagttt aaattctaca ttgatgggga tagtgagtat
    23821 ccgaccgtaa cctcaacagg ttcagaagat tattttggcg gggcgtgggc ctttcatgag
    23881 tacgatgagt tcggacgtcc acatgcgaaa aactattcaa cagccttttt agggtatcct
    23941 taccaatcaa atagagatgc cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt
    24001 catgcctttg gtgacgacgc aatgccacga catggtcttt atcgttggca tcttcaagac
    24061 cccatctgtt ttaccgaaga tttatcggtc actttacaac aaatcggcaa cgatgatgtt
    24121 cgcttatttg agcggcaaga cgatgttgct agtgtcgctt actggtattc aacagaagct
    24181 tcaggaaata ctaaacgtca tttcactgat cgagaacgct tgccgcgtta atgaagtgtt
    24241 tttcacatat tcgaagggag gaacctatga tgatatatgg ggcaattgaa gctggaggga
    24301 ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat tttggaaaga gtcagttttc
    24361 ccacaacaac tccagttgaa acaatgaagc aggtcattga tttttttaaa ccttttaagg
    24421 aacaattggc aggcatcggg gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa
    24481 cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc aattttgtag
    24541 gaacgctaaa gcaatatttc aatattccga ttgaatggac aacagatgtc aatgcggctt
    24601 gctatggtga atatgttgct ggttctggca aagggttagc aggtgttgca tacttcactg
    24661 tgggaacagg tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc
    24721 atccggaaat ggggcatatg ttgatcaagc gacatccaaa ggacaatttt tcaggaaact
    24781 gtcccttcca ccaagattgt ttagagggga tggcagcagg accagcgatt gaaaaacggt
    24841 tgggtgtaaa aggccagaat ttattggctg acgattcgtt ttggcagatt gaagcctttt
    24901 accttgccca atgtgcatac aatacaacac tgatgttttc gccagataga attatttttg
    24961 gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt tcaggataaa tttgttgagc
    25021 ttataaatgg atatgttgaa ataccaccaa ttgatagcta cattattacg cccgaattag
    25081 gtgataatgc cggaataatc ggcggattag cattggccag aaaagcggta agaaataagc
    25141 aaccataatt aatgaatgtt tcattgatac tcatgtcaaa agtatttaac cgagtaatca
    25201 aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa
    25261 atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct tttttctttt
    25321 tgttgtgcgc tgtatacctt gaggatattc actggtggcg tacacttaaa tagtaatata
    25381 ttgtgttact ttatgtctgt ggtaatagtt atagttgctt accaatgtat gttcattttt
    25441 cgaaatgttg acttcataat ccttggctta atgtttgaat ttttttggtg atagattttt
    25501 tcagccgtat aaaataaatc gtaattctat tatagagagt tgtgattata aaaagatagc
    25561 aagaaaaaaa aagaaagttt ctatacattt taaatatttt attcctattt ctattgctaa
    25621 aaaagtagaa attctatatt tacctttaac tctcgcttct cttgcaaatg tagttagcat
    25681 tttaatcgat aataaactca ggtagttata ttaccttgaa atgattgata attttgtcac
    25741 ttctattttt tttgcaataa attttttatt ttagtgaaaa ataagaaact atttctttga
    25801 aggcgataag ttagaaaaat attattgtag tcttttcttt a
//
LOCUS       DAZKOK010000025        25690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000025 DAZKOK010000000
VERSION     DAZKOK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.025"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..3649
                     /locus_tag="ACG90W_000966"
     CDS             122..3649
                     /locus_tag="ACG90W_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0477345.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3646..7368
                     /gene="addA"
                     /locus_tag="ACG90W_000967"
     CDS             3646..7368
                     /gene="addA"
                     /locus_tag="ACG90W_000967"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0477346.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7437..7778
                     /locus_tag="ACG90W_000968"
     CDS             7437..7778
                     /locus_tag="ACG90W_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0477347.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8087..9130
                     /gene="pheS"
                     /locus_tag="ACG90W_000969"
     CDS             8087..9130
                     /gene="pheS"
                     /locus_tag="ACG90W_000969"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0477348.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9135..11555
                     /gene="pheT"
                     /locus_tag="ACG90W_000970"
     CDS             9135..11555
                     /gene="pheT"
                     /locus_tag="ACG90W_000970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0477349.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11725..12735)
                     /gene="trpS"
                     /locus_tag="ACG90W_000971"
     CDS             complement(11725..12735)
                     /gene="trpS"
                     /locus_tag="ACG90W_000971"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0477350.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12989..13726
                     /locus_tag="ACG90W_000972"
     CDS             12989..13726
                     /locus_tag="ACG90W_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477351.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13740..14555
                     /locus_tag="ACG90W_000973"
     CDS             13740..14555
                     /locus_tag="ACG90W_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0477352.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14568..15215
                     /locus_tag="ACG90W_000974"
     CDS             14568..15215
                     /locus_tag="ACG90W_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0477353.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15231..15887
                     /locus_tag="ACG90W_000975"
     CDS             15231..15887
                     /locus_tag="ACG90W_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0477354.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16049..16870
                     /gene="racE"
                     /locus_tag="ACG90W_000976"
     CDS             16049..16870
                     /gene="racE"
                     /locus_tag="ACG90W_000976"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0477355.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16873..18228
                     /gene="rph"
                     /locus_tag="ACG90W_000977"
     CDS             16873..18228
                     /gene="rph"
                     /locus_tag="ACG90W_000977"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0477356.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18229..18747
                     /locus_tag="ACG90W_000978"
     CDS             18229..18747
                     /locus_tag="ACG90W_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0477357.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18854..19354
                     /gene="cbpB"
                     /locus_tag="ACG90W_000979"
     CDS             18854..19354
                     /gene="cbpB"
                     /locus_tag="ACG90W_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0477358.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19634..20554)
                     /locus_tag="ACG90W_000980"
     CDS             complement(19634..20554)
                     /locus_tag="ACG90W_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0477359.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20680..21732
                     /locus_tag="ACG90W_000981"
     CDS             20680..21732
                     /locus_tag="ACG90W_000981"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0477360.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21842..22597)
                     /gene="fabI"
                     /locus_tag="ACG90W_000982"
     CDS             complement(21842..22597)
                     /gene="fabI"
                     /locus_tag="ACG90W_000982"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0477361.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22817..23257
                     /gene="fabZ"
                     /locus_tag="ACG90W_000983"
     CDS             22817..23257
                     /gene="fabZ"
                     /locus_tag="ACG90W_000983"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0477362.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23372..24133)
                     /locus_tag="ACG90W_000984"
     CDS             complement(23372..24133)
                     /locus_tag="ACG90W_000984"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0477363.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24322..25416
                     /locus_tag="ACG90W_000985"
     CDS             24322..25416
                     /locus_tag="ACG90W_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0477364.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25502..>25690)
                     /locus_tag="ACG90W_000986"
     CDS             complement(25502..>25690)
                     /locus_tag="ACG90W_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0477365.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8508 a         4454 c         5415 g         7313 t
ORIGIN      
        1 acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa tatagaacaa
       61 atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa ggaggaaaag
      121 catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag tcttgatcga
      181 cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc ttgttcccaa
      241 ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa gaagcggaaa
      301 aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg cttggtattt
      361 gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat ctgcaatgat
      421 cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg gagaaatcaa
      481 taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta caggaaatat
      541 tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag accagcaact
      601 taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga ctcaacgtgc
      661 actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac aagatttcaa
      721 caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt atcaattact
      781 gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc gtccttatac
      841 agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt atttccgtct
      901 ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg caccagagca
      961 gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat ctagccaaaa
     1021 gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg aagctgaaaa
     1081 tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca gtgaagaaaa
     1141 ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg gaaatatcct
     1201 tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac aaatgatgga
     1261 aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct attcttatcg
     1321 gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg aagcagaaac
     1381 cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg acatcactga
     1441 aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa aagactggca
     1501 gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt tggaagaaca
     1561 gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga aaaaaataaa
     1621 gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga tccaaagtgg
     1681 cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa acttagaagc
     1741 ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg aatatgtagt
     1801 aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta caagtggtct
     1861 agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa tcaatcgttt
     1921 ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga atgatcaagt
     1981 gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag aagtattaaa
     2041 tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta tctcttttga
     2101 gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc ttagttatgc
     2161 acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta gaatcacaga
     2221 atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg accctaattg
     2281 ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc gattagcttt
     2341 agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag tactgaattc
     2401 gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa atgttcctgt
     2461 ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata cttcaatttc
     2521 acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg gtttgaaatt
     2581 gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt atcatgaatc
     2641 attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga atatgtccga
     2701 tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag aacttcggtt
     2761 tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag gacagacgat
     2821 tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc atcctcttca
     2881 gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc tggaattacc
     2941 gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg atgtagcttc
     3001 tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac gttcattcga
     3061 tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg acgtagcatt
     3121 gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat catattattt
     3181 acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata cattgaaaaa
     3241 atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac tagatcattc
     3301 actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga aagatgaata
     3361 ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc aactattcat
     3421 ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg gagaaatcaa
     3481 actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc cattcagaag
     3541 catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg agaaaataga
     3601 taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa tcaaattcct
     3661 ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt tgatcaagga
     3721 gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct tgttcgaaga
     3781 gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt gacatttact
     3841 gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga atccgtcaat
     3901 agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt attgcccaca
     3961 gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta ttattactta
     4021 attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat cttgatgaag
     4081 gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga acgatttttc
     4141 caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa tctggtgttt
     4201 tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga acagttaagc
     4261 gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa acaaatccgt
     4321 cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat gactcagtta
     4381 gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca acagcagatc
     4441 aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc tggtttggaa
     4501 caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga aatttccaat
     4561 gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag taaaagttat
     4621 tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc gttagtcgaa
     4681 gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa acgggaaaaa
     4741 ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac agaaaaaacg
     4801 aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt caaagaagta
     4861 atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata ctggctaaga
     4921 gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca atccatctat
     4981 tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt ctcaaagaaa
     5041 gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga agtcctttct
     5101 tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat caattatgac
     5161 gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca gtttgagccg
     5221 gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc gacggatgat
     5281 atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat tcgtcaatta
     5341 attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc gatagagtac
     5401 aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt agagattttc
     5461 aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca agcgacagaa
     5521 attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga tattccgctt
     5581 gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc cagcattcga
     5641 ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc atcaaacgaa
     5701 gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg gcgagaactt
     5761 gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac gggatatcta
     5821 gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta tgcattggtt
     5881 gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt cgtgcgtttc
     5941 attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc aatagaagat
     6001 aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc agttgtattt
     6061 ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta tgcttttgaa
     6121 gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt atatgatacg
     6181 ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga agaaatgcgt
     6241 aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg ttcctataaa
     6301 aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga gttagttttt
     6361 gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg ttacggattg
     6421 atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc tcttcttcaa
     6481 cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat tgaacaaaga
     6541 cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga agatgaaact
     6601 ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca agcttcttca
     6661 caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga tccagatgat
     6721 acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc ccgtcaattt
     6781 cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg gcaagtgtca
     6841 gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt agagatgcca
     6901 actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt agtagacgaa
     6961 aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac agaacttggt
     7021 caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc catgctgctt
     7081 cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt aatccacgga
     7141 atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt taaaacagat
     7201 tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa atatcaagga
     7261 cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt agaaaatgtt
     7321 ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt ataattgagc
     7381 aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg agtatgatgg
     7441 agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc tctattttag
     7501 gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg caacgctttg
     7561 ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa cgcttgaagg
     7621 aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag agccgttcag
     7681 aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa atacaagttt
     7741 ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa aatcttgtaa
     7801 actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt ggctaaacca
     7861 gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat gaagttcact
     7921 ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta agagtgcggt
     7981 gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct taacaggaat
     8041 gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt ctctacaagc
     8101 acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac aagatttaaa
     8161 tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca cagaagtatt
     8221 acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt tcgcaaatga
     8281 gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag aagaagaagc
     8341 gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta gaaaagtaaa
     8401 acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata tttttgtggg
     8461 tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca atttcgaacg
     8521 gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct atatttcaga
     8581 cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg aaaaacatga
     8641 tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca gagatacaga
     8701 tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg ataaaaacat
     8761 cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt ttggggaaga
     8821 tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag tagaagtaga
     8881 tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca ctggatggat
     8941 cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag gaatcgatcc
     9001 agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta tgttgcggta
     9061 tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa gtcagttcaa
     9121 gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta gatttatcga
     9181 atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa gtagagggag
     9241 taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag gaatgcgtac
     9301 cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa gacgaactgt
     9361 ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt gttgcacttc
     9421 ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt ggccaagtat
     9481 caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt ataccgaaag
     9541 aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat ccagtttttc
     9601 cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat cgtgcggatg
     9661 cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag actcctacat
     9721 tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt caagtggaag
     9781 ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga gtaagaatcc
     9841 agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt ccaatcaaca
     9901 atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta catgcatttg
     9961 actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa aatgaaacat
    10021 tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc attactaacg
    10081 gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag atcacgaatg
    10141 aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt cgtcgtacag
    10201 caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga atcaaccatg
    10261 cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt gcgggtgggg
    10321 aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta tcagtgaaga
    10381 tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa gaagtaaatg
    10441 agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat tccgtagtag
    10501 taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa gttgctcgta
    10561 tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt gctggaagct
    10621 tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga agcggattga
    10681 gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt gttataaatg
    10741 aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta ttacgaatga
    10801 atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa aatacagaga
    10861 ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt acacatctgc
    10921 cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa aaagattggc
    10981 agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa aacttatttg
    11041 aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg aaggaaatgc
    11101 atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt gtcggtcaag
    11161 tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct gagtttgatt
    11221 tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg acaaaattcc
    11281 cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat caggaattaa
    11341 cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta tttgatgtct
    11401 atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg acattcgtca
    11461 acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa gtcgaaaaag
    11521 cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt aagtaacgat
    11581 caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt cgtcacatct
    11641 ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca ggcctttaca
    11701 gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg ctgattttac
    11761 ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac gtaagatttc
    11821 cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg gtgcaaattc
    11881 agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc cgccgcggcg
    11941 gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat ccaagtaggt
    12001 gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt ttggatcagt
    12061 atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat aaataccgtt
    12121 tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac ccattccttt
    12181 tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat tgaagctttg
    12241 tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag gtacaagatt
    12301 cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga atcctgtagg
    12361 cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat tgcgccgaac
    12421 gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt ccggaatttg
    12481 tgattggata aataaagtag attttgcagg atctaatccc acggctaaat agtctaaagc
    12541 aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg tcagtgcttg
    12601 catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag ccactcgggt
    12661 ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat ctcccgttaa
    12721 aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca cttaatatct
    12781 taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa agaggataaa
    12841 ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt ctaagaaact
    12901 ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta aaagaaatct
    12961 tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc acgtggataa
    13021 atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga aaggagaagt
    13081 tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct gtatcaatgg
    13141 attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc atgataaaaa
    13201 gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt tcaatttgta
    13261 cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt tgaagcaaga
    13321 ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata tgttagataa
    13381 gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg caatcgccag
    13441 aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg ctttggatcc
    13501 ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg gtatgtcaat
    13561 gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg ttatcttcat
    13621 ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg aaaatccaaa
    13681 tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg aggaaaaaga
    13741 tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt cttgcactag
    13801 ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc aaatctacca
    13861 atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg gatattgaga
    13921 gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa aagatccttg
    13981 gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt ccattattga
    14041 aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa cgaaaaaaac
    14101 aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta aagaaaggaa
    14161 gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca gtcaaaggtt
    14221 ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta gaattagaaa
    14281 actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg actacggata
    14341 atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt ggtggaacgt
    14401 ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc ttgaaagctg
    14461 ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc tatcaaaaat
    14521 ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg agcgaattga
    14581 tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt tcgagtgtct
    14641 tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa ttatcaaaga
    14701 ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac attccattat
    14761 taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat ctagatggat
    14821 tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct gaaacagtac
    14881 gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct tctggtttta
    14941 cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc gttatcccgc
    15001 cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca atcgtagctg
    15061 gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat accttcgata
    15121 cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct tacttaatgg
    15181 tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac atggatttta
    15241 gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag gttactgttg
    15301 aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca ggtgtgattc
    15361 gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat attatacgga
    15421 atcttccatt attattgatc attttcttca cttactttgc cttacctcaa atcggcattc
    15481 agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca gctatgttgt
    15541 cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa gcaggattat
    15601 caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa gcatttaaat
    15661 caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact tcattagcag
    15721 taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga cagaacacca
    15781 attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta tgttacgcgc
    15841 tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag atgattcttt
    15901 tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg aaaaaaagag
    15961 atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa gtacacaata
    16021 gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc gccctatcgg
    16081 atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga aacaattacc
    16141 gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc ctagacccgc
    16201 ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc aaggaatcaa
    16261 gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa tcaaagctgc
    16321 tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag taaaaaaaac
    16381 acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc aagcttatga
    16441 aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt gtcccaaatt
    16501 tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa ttgtggcaga
    16561 aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat gcacccatta
    16621 tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac tgatcgattc
    16681 tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt tgagcaattc
    16741 accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca aacttttcga
    16801 ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata tcgaattggg
    16861 aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa actggagctt
    16921 caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg tgatacaaca
    16981 gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga aacaggaaca
    17041 ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag aaatcgtaga
    17101 gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct tattggtcgt
    17161 tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat cgtagactgc
    17221 gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc atttgtggca
    17281 ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga tccaataaaa
    17341 gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt cacagatttg
    17401 gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac agaatctgga
    17461 aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga acagttgaat
    17521 gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca aaaacatgct
    17581 ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac aatcatcatt
    17641 gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga agcgggatat
    17701 catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac tggcagcaca
    17761 tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga tcagccagtc
    17821 ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg aatctattct
    17881 gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt attatatgaa
    17941 ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt agtattcgcg
    18001 gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt agcccgtata
    18061 ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg taagaaacaa
    18121 acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc tcaagcaatg
    18181 aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat gcggtattta
    18241 gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga tcgttatcgt
    18301 tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc tgacccatta
    18361 tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt cgaacagaaa
    18421 aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt atcgaatgtc
    18481 cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga tatggtgttt
    18541 tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt tttgaatcct
    18601 ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct tatcgaaagt
    18661 ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt tgaagagttg
    18721 gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat aaaaataagc
    18781 tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat gaaatagaaa
    18841 tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa atcaggaaac
    18901 gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat tgtcccatgg
    18961 tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa aagatgatca
    19021 ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact tgcaaacaat
    19081 cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag atgttccgac
    19141 aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt cgcctttttt
    19201 accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag agctgcttaa
    19261 agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc cgaaaatcga
    19321 agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa agataatcaa
    19381 aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag aggctgggac
    19441 aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt caaatttttg
    19501 tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata cgtgtttaga
    19561 gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag attcgttgca
    19621 tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg aaaccagaag
    19681 ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat tgttttccgt
    19741 tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc actaatccaa
    19801 agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta cgatttactc
    19861 gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga atatctaaaa
    19921 gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga ttcgggatga
    19981 aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca gatacgacgg
    20041 ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc tgttgttcca
    20101 tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca agaccgattg
    20161 ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg atagcagata
    20221 aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt aaaagactat
    20281 gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga ttaaaagact
    20341 tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc aaaaaaatca
    20401 gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc ccgtcccaaa
    20461 accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg ccagaagagt
    20521 ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta aaatttgatt
    20581 ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa ctataatgaa
    20641 ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg tggagaaaat
    20701 aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc ttatatcaaa
    20761 actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg agaattgaca
    20821 gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg tcagcaatgt
    20881 tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt tcgacctgaa
    20941 caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat ggtccctgaa
    21001 aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt tgtgccgccc
    21061 tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg tgaaaacatt
    21121 ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt aggagcgtat
    21181 ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg tgctgctcct
    21241 aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact tccaggaaca
    21301 gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac acatacgaaa
    21361 atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa attcatcact
    21421 ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca gttagctgaa
    21481 gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct agatcaattt
    21541 gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg gatttatcac
    21601 aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac aaaacggttg
    21661 tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg ctggattgaa
    21721 gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa aatcaggaag
    21781 agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat gcaacaaaag
    21841 tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt ccgctggaaa
    21901 gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca ccttgctgat
    21961 caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca cctgtgacag
    22021 ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta ggcgagaact
    22081 cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc atcatgttat
    22141 aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg ctgtttggtg
    22201 caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg atatcttgag
    22261 ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct tctttgttgg
    22321 cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg atggcattca
    22381 ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat gcttcttcgc
    22441 cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag ataatttctg
    22501 ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg ttagcgacgc
    22561 ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt tgtttctgat
    22621 aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc ttgatttctt
    22681 aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa aaaaagattg
    22741 ctaaatattt aggaatatga tactttgata gtcaaagtaa tgaaaatggc aattgcctga
    22801 aagaaggagt ttaattatgt cttacgtatt atctgctact gaagtaatgg aattgattcc
    22861 taatcgttat cctatttgtt atattgatta tgtagatact ttagaaccag gaaaaaagat
    22921 cgttgcgaca aaaaatgtaa caatcaatga agattttttc caaggtcact ttcctgacaa
    22981 tccagtgatg ccgggtgcgt tgattctaga aacactggca caagcaggtt cgatacttat
    23041 cctaaaatca gaagagtttc taggaaaaag agcttatatc ggtgggatca ataaggccaa
    23101 attccgtcaa aaagttgttc caggagatgt gctgaaatgt cattttgaaa tcaccaaaat
    23161 gaagggatca gtcggtacag cgttatctgc agcatatgta gatgataaaa aagtctgtga
    23221 gtgcgagttc acgttcatcg tcaaccaaaa tgcttgacta taaaacctga tatagcaaaa
    23281 caaaaggaag cttacttgct ttttatgtaa aaagaagacg cctgaatcct agcatacgtt
    23341 gtcatctatg caggattcag acgttatttt tttatttttg ttttgcttct tgcagtatat
    23401 tgattttatc gatttttact tcttcttttg gtttgtctga actatctgtt tcgatatttg
    23461 caatagcatc cacgacatcc attccttcaa tcacttggcc gaagacagta taatggccat
    23521 ctagtgaagg ataaccgccg tttttataag cgtcgatgat tgcttgagga tagtcgtctt
    23581 ttaaaagacc atcatgcata tcatctgtat tttgaacgat aaagaactgg ctgccgttac
    23641 tgttaggatc ttgtgagcga gccatagata acgcacctct tatattataa aggtggttgt
    23701 tgatctctgt ttcaaatcct tctccccaaa tgctttcccc tccagtgcca tcgcctttag
    23761 gatcgccacc ttggatcatg aaatctttga tgacgcgatg gaaagtcagt ccgttgtagt
    23821 aaccgtcttt tgcatgtgtc ataaagtttt caaccgcttt tggtgcgtgt tcgggaaata
    23881 atttgatttt gattgtgcct ttattcgtca ccatttcgac cagatcttca ttttcggcga
    23941 catctttcgt caattgtggt aattccaatg cgttcaaatc gacgctactg gcagtcgtgc
    24001 tttccgcagt agatgtactt tttgaacttt ctgtagacga actagaagaa gtcgtgttgt
    24061 ttgcagaaca accagcagca aacagcaatg ctgctgctaa actaatactt aaaataactt
    24121 tatttttctt cataactgtt cctttccaat ttactatttt gttatcttta atgataacaa
    24181 aatagaggga aaatacctac ttaaattatt ggaatttatc atttatttta aaagaaaaag
    24241 aaaaggtgta gaccatttac tttgtgtttg ctataatgta agtggataca aaaacgaaaa
    24301 gatgcaggag gaaatggtaa tatgggaaaa ttaggaatct cgatttatcc agaacggtca
    24361 acatttgaaa aggacaaagc gtacttggat ttagctcata agtatgggta taaacgggtt
    24421 tttaccagtt tgctgcagat caatgatgat aaagaaaaag ttctttcaga atttaaagaa
    24481 gtagttgatt acgcgaatag cctaggcatg gaagtaatgg ttgatatcaa tccagcgtta
    24541 tttgaacagt tggagatttc ttacgatgac ttatcttttt tccataagat gggtgcctac
    24601 ggggtccgct tagatatcgg attcacagga gcagaagaag caaaaatgac gcgcaatcct
    24661 tttggtatca aaatcgagat caatatgagt tcgggcacga attacgttga caacatcatg
    24721 agttattcgc caaacacgga taatttatta ggttctcata atttttatcc tcatcgatac
    24781 tctggtttag gatacgaaca ctttgttttt tgttcagaaa aattcagaaa atataatttg
    24841 aatacgatgg cattcgtcaa ttcgcaatct gctgagtttg gcccttggcc tacgcaagat
    24901 ggcttgtgca cgctggaaga ccaccgagat cttgaaattg cgacacaagt gaagcattta
    24961 atccttacag gattgattga tgatatttct attggaaatg cgtacgcaag tgaagaagaa
    25021 ttgaaagaaa tggcagaagc atttaacgcg gattatccaa cattgaaagt agatacggaa
    25081 gaaggtatta cagaaaacga acgaatctgc ttgtttgata accttcatag ttatcgaggg
    25141 gatcgctcag aatatatcct tcgttcaacg atgactcgtg tatactacaa agataaagat
    25201 ttcccgcctc ataatacgcg agatatgcat catggggatg tactgattga caatgaaggg
    25261 tacggtcaat acaaaggaga aacacaaatc gccttgaagg atatgaaaaa cgacggacgt
    25321 gtcaatgttg taggaagaat ctccgatgac gaattattcc tccttgactt tttgaaacca
    25381 tggtcaagct ttaaattaat tgaaaataaa aaatagataa ttagttattg cagaaataat
    25441 ccgctagaaa tagccagtta tttctgcttt tttattttgt ttttttatag gttatgattt
    25501 gtcaaattaa gctagaataa aatgcttctt gtacatagct caaaaatatt tcgattggtg
    25561 ttctgtaatt caatgatttt cttggaatat gattacgttg attgctgaca ctggaaataa
    25621 atgtctgatt cacttttcta aaatccattg atttcggcag tccattacga cgcagaatcc
    25681 cgttagaatt
//
LOCUS       DAZKOK010000026        25242 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000026 DAZKOK010000000
VERSION     DAZKOK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.026"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG90W_000987"
     CDS             complement(164..388)
                     /locus_tag="ACG90W_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0477366.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG90W_000988"
     CDS             complement(586..2166)
                     /locus_tag="ACG90W_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0477367.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG90W_000989"
     CDS             complement(2567..3943)
                     /locus_tag="ACG90W_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0477368.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG90W_000990"
     CDS             complement(4071..5159)
                     /locus_tag="ACG90W_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0477369.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG90W_000991"
     CDS             5331..5765
                     /locus_tag="ACG90W_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477370.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG90W_000992"
     CDS             complement(5843..6376)
                     /locus_tag="ACG90W_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0477371.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG90W_000993"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG90W_000993"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0477372.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG90W_000994"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG90W_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0477373.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG90W_000995"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG90W_000995"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0477374.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG90W_000996"
     CDS             complement(10101..10679)
                     /locus_tag="ACG90W_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0477375.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG90W_000997"
     CDS             11001..11471
                     /locus_tag="ACG90W_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0477376.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG90W_000998"
     CDS             complement(11579..12805)
                     /locus_tag="ACG90W_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0477377.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG90W_000999"
     CDS             complement(13559..13759)
                     /locus_tag="ACG90W_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0477378.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG90W_001000"
     CDS             complement(13893..14501)
                     /locus_tag="ACG90W_001000"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0477379.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG90W_001001"
     CDS             complement(14586..15056)
                     /locus_tag="ACG90W_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0477380.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG90W_001002"
     CDS             complement(15104..16018)
                     /locus_tag="ACG90W_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0477381.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG90W_001003"
     CDS             16164..16967
                     /locus_tag="ACG90W_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0477382.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG90W_001004"
     CDS             17145..18092
                     /locus_tag="ACG90W_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0477383.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG90W_001005"
     CDS             complement(18160..18453)
                     /locus_tag="ACG90W_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0477384.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG90W_001006"
     CDS             complement(18481..19146)
                     /locus_tag="ACG90W_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0477385.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG90W_001007"
     CDS             complement(19286..19918)
                     /locus_tag="ACG90W_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0477386.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG90W_001008"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG90W_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0477387.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG90W_001009"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG90W_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0477388.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG90W_001010"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG90W_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0477389.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG90W_001011"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG90W_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0477390.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACG90W_001012"
     CDS             23883..25052
                     /locus_tag="ACG90W_001012"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0477391.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7582 a         5092 c         4062 g         8506 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc caaattattc taagatttct ct
//
LOCUS       DAZKOK010000027        24419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000027 DAZKOK010000000
VERSION     DAZKOK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.027"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..911)
                     /locus_tag="ACG90W_001013"
     CDS             complement(63..911)
                     /locus_tag="ACG90W_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0477392.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="ACG90W_001014"
     CDS             complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="ACG90W_001014"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0477393.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3412..3591)
                     /locus_tag="ACG90W_001015"
     CDS             complement(3412..3591)
                     /locus_tag="ACG90W_001015"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0477394.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3638..5461)
                     /locus_tag="ACG90W_001016"
     CDS             complement(3638..5461)
                     /locus_tag="ACG90W_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0477395.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5739..8018
                     /gene="helD"
                     /locus_tag="ACG90W_001017"
     CDS             5739..8018
                     /gene="helD"
                     /locus_tag="ACG90W_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0477396.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8111..8758)
                     /locus_tag="ACG90W_001018"
     CDS             complement(8111..8758)
                     /locus_tag="ACG90W_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477397.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8937..9656)
                     /locus_tag="ACG90W_001019"
     CDS             complement(8937..9656)
                     /locus_tag="ACG90W_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0477398.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9787..10938)
                     /locus_tag="ACG90W_001020"
     CDS             complement(9787..10938)
                     /locus_tag="ACG90W_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0477399.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11155..11895)
                     /locus_tag="ACG90W_001021"
     CDS             complement(11155..11895)
                     /locus_tag="ACG90W_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0477400.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="ACG90W_001022"
     CDS             complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="ACG90W_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0477401.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="ACG90W_001023"
     CDS             complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="ACG90W_001023"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0477402.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12856..13506)
                     /locus_tag="ACG90W_001024"
     CDS             complement(12856..13506)
                     /locus_tag="ACG90W_001024"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0477403.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="ACG90W_001025"
     CDS             complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="ACG90W_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0477404.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="ACG90W_001026"
     CDS             complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="ACG90W_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0477405.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14949..15476)
                     /locus_tag="ACG90W_001027"
     CDS             complement(14949..15476)
                     /locus_tag="ACG90W_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0477406.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15656..16441)
                     /locus_tag="ACG90W_001028"
     CDS             complement(15656..16441)
                     /locus_tag="ACG90W_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0477407.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16434..17303)
                     /gene="accD"
                     /locus_tag="ACG90W_001029"
     CDS             complement(16434..17303)
                     /gene="accD"
                     /locus_tag="ACG90W_001029"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0477408.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17304..18683)
                     /locus_tag="ACG90W_001030"
     CDS             complement(17304..18683)
                     /locus_tag="ACG90W_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0477409.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="ACG90W_001031"
     CDS             complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="ACG90W_001031"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0477410.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19135..19611)
                     /gene="accB"
                     /locus_tag="ACG90W_001032"
     CDS             complement(19135..19611)
                     /gene="accB"
                     /locus_tag="ACG90W_001032"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0477411.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="ACG90W_001033"
     CDS             complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="ACG90W_001033"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0477412.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="ACG90W_001034"
     CDS             complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="ACG90W_001034"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0477413.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="ACG90W_001035"
     CDS             complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="ACG90W_001035"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0477414.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22641..22865)
                     /locus_tag="ACG90W_001036"
     CDS             complement(22641..22865)
                     /locus_tag="ACG90W_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0477415.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22918..23880)
                     /locus_tag="ACG90W_001037"
     CDS             complement(22918..23880)
                     /locus_tag="ACG90W_001037"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0477416.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23890..24330)
                     /locus_tag="ACG90W_001038"
     CDS             complement(23890..24330)
                     /locus_tag="ACG90W_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0477417.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6684 a         5433 c         4252 g         8050 t
ORIGIN      
        1 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
       61 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
      121 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
      181 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
      241 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
      301 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
      361 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
      421 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
      481 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
      541 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
      601 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
      661 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
      721 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
      781 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
      841 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
      901 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
      961 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     1021 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     1081 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     1141 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     1201 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     1261 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     1321 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     1381 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     1441 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     1501 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     1561 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     1621 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     1681 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     1741 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     1801 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     1861 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     1921 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     1981 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     2041 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     2101 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     2161 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     2221 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     2281 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     2341 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     2401 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     2461 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     2521 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     2581 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     2641 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     2701 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     2761 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     2821 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     2881 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     2941 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     3001 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     3061 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     3121 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     3181 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     3241 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     3301 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     3361 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     3421 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     3481 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     3541 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     3601 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     3661 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     3721 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     3781 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     3841 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     3901 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     3961 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     4021 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     4081 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     4141 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     4201 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     4261 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     4321 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     4381 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     4441 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     4501 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     4561 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     4621 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     4681 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     4741 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     4801 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     4861 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     4921 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     4981 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     5041 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     5101 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     5161 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     5221 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     5281 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     5341 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     5401 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     5461 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     5521 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     5581 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     5641 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     5701 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     5761 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     5821 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     5881 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     5941 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     6001 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     6061 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     6121 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     6181 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     6241 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     6301 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     6361 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     6421 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     6481 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     6541 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     6601 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     6661 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     6721 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     6781 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     6841 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     6901 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     6961 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     7021 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     7081 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     7141 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     7201 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     7261 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     7321 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     7381 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     7441 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
     7501 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
     7561 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
     7621 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
     7681 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
     7741 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
     7801 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
     7861 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
     7921 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
     7981 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
     8041 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
     8101 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
     8161 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
     8221 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
     8281 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
     8341 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
     8401 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
     8461 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
     8521 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
     8581 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
     8641 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
     8701 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
     8761 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
     8821 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
     8881 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
     8941 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
     9001 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
     9061 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
     9121 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
     9181 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
     9241 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
     9301 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
     9361 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
     9421 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
     9481 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
     9541 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
     9601 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
     9661 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
     9721 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
     9781 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
     9841 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
     9901 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
     9961 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    10021 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    10081 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    10141 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    10201 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    10261 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    10321 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    10381 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    10441 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    10501 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    10561 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    10621 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    10681 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    10741 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    10801 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    10861 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    10921 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    10981 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    11041 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    11101 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    11161 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    11221 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    11281 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    11341 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    11401 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    11461 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    11521 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    11581 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    11641 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    11701 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    11761 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    11821 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    11881 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    11941 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    12001 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    12061 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    12121 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    12181 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    12241 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    12301 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    12361 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    12421 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    12481 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    12541 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    12601 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    12661 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    12721 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    12781 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    12841 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    12901 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    12961 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    13021 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    13081 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    13141 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    13201 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    13261 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    13321 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    13381 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    13441 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    13501 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    13561 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    13621 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    13681 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    13741 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    13801 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    13861 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    13921 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    13981 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    14041 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    14101 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    14161 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    14221 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    14281 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    14341 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    14401 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    14461 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    14521 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    14581 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    14641 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    14701 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    14761 caaaaagtcg tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa
    14821 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    14881 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    14941 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    15001 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    15061 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    15121 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    15181 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    15241 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    15301 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    15361 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    15421 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    15481 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    15541 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    15601 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    15661 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    15721 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    15781 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    15841 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    15901 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    15961 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    16021 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    16081 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    16141 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    16201 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    16261 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    16321 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    16381 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    16441 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    16501 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    16561 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    16621 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    16681 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    16741 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    16801 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    16861 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    16921 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    16981 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    17041 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    17101 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    17161 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    17221 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    17281 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    17341 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    17401 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    17461 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    17521 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    17581 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    17641 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    17701 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    17761 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    17821 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    17881 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    17941 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    18001 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    18061 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    18121 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    18181 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    18241 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    18301 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    18361 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    18421 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    18481 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    18541 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    18601 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    18661 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    18721 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    18781 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    18841 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    18901 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    18961 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    19021 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    19081 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    19141 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    19201 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    19261 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    19321 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    19381 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    19441 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    19501 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    19561 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    19621 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    19681 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    19741 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    19801 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    19861 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    19921 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    19981 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    20041 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    20101 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    20161 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    20221 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    20281 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    20341 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    20401 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    20461 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    20521 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    20581 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    20641 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    20701 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    20761 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    20821 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    20881 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    20941 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    21001 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    21061 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    21121 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    21181 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    21241 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    21301 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    21361 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    21421 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    21481 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    21541 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    21601 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    21661 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    21721 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    21781 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    21841 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    21901 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    21961 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    22021 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    22081 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    22141 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    22201 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    22261 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    22321 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    22381 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    22441 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    22501 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    22561 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    22621 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    22681 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    22741 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    22801 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    22861 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    22921 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    22981 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    23041 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    23101 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    23161 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    23221 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    23281 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    23341 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    23401 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    23461 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    23521 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    23581 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    23641 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    23701 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    23761 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    23821 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    23881 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    23941 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    24001 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    24061 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    24121 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    24181 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    24241 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    24301 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    24361 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagat
//
LOCUS       DAZKOK010000028        24331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000028 DAZKOK010000000
VERSION     DAZKOK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.028"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..2471)
                     /locus_tag="ACG90W_001039"
     CDS             complement(126..2471)
                     /locus_tag="ACG90W_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0477418.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2750..3652
                     /locus_tag="ACG90W_001040"
     CDS             2750..3652
                     /locus_tag="ACG90W_001040"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0477419.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3863..5638
                     /locus_tag="ACG90W_001041"
     CDS             3863..5638
                     /locus_tag="ACG90W_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0477420.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5704..7290)
                     /locus_tag="ACG90W_001042"
     CDS             complement(5704..7290)
                     /locus_tag="ACG90W_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0477421.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7541..7765
                     /locus_tag="ACG90W_001043"
     CDS             7541..7765
                     /locus_tag="ACG90W_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0477422.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7907..9658
                     /locus_tag="ACG90W_001044"
     CDS             7907..9658
                     /locus_tag="ACG90W_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0477423.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9658..11442
                     /locus_tag="ACG90W_001045"
     CDS             9658..11442
                     /locus_tag="ACG90W_001045"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477424.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11777..12629
                     /gene="licT"
                     /locus_tag="ACG90W_001046"
                     /pseudo
     CDS             11777..12629
                     /gene="licT"
                     /locus_tag="ACG90W_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12726..14612
                     /locus_tag="ACG90W_001047"
     CDS             12726..14612
                     /locus_tag="ACG90W_001047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0477425.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14653..15555)
                     /locus_tag="ACG90W_001048"
     CDS             complement(14653..15555)
                     /locus_tag="ACG90W_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0477426.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15785..17188
                     /locus_tag="ACG90W_001049"
     CDS             15785..17188
                     /locus_tag="ACG90W_001049"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0477427.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17188..18621
                     /locus_tag="ACG90W_001050"
     CDS             17188..18621
                     /locus_tag="ACG90W_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0477428.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18766..19692
                     /gene="trxB"
                     /locus_tag="ACG90W_001051"
     CDS             18766..19692
                     /gene="trxB"
                     /locus_tag="ACG90W_001051"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0477429.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19905..21632
                     /locus_tag="ACG90W_001052"
     CDS             19905..21632
                     /locus_tag="ACG90W_001052"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0477430.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21648..21998
                     /locus_tag="ACG90W_001053"
     CDS             21648..21998
                     /locus_tag="ACG90W_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0477431.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22092..22952)
                     /locus_tag="ACG90W_001054"
     CDS             complement(22092..22952)
                     /locus_tag="ACG90W_001054"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0477432.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23044..23472
                     /locus_tag="ACG90W_001055"
     CDS             23044..23472
                     /locus_tag="ACG90W_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0477433.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23634..23810
                     /gene="rpsU"
                     /locus_tag="ACG90W_001056"
     CDS             23634..23810
                     /gene="rpsU"
                     /locus_tag="ACG90W_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0477434.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23837..24283
                     /locus_tag="ACG90W_001057"
     CDS             23837..24283
                     /locus_tag="ACG90W_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0477435.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5185 g         7085 t
ORIGIN      
        1 cacattttta cataaatttg tcttaatatc gtagatgtaa gcttttgaac accatttatt
       61 cggaaaaagg gagctgtgac aagatttttg tcgcagctcc ctttaattct gattaatcta
      121 ttagatcaat catctttatc gtctgattct ttggtctctg gattcgctct agcataagct
      181 gaagcggtgt tccagtaatc tgtatagttt tcatcagtac cgcctacgcc gaatttaaag
      241 gcgcttttct tcggaccatt ttttgcccaa agtgactcca ctgtttttcc tggcgtttgg
      301 atggattttt tattgacagt aacttttcca gctgtttgtc ctgtgaaccc agatactttt
      361 ttcttcatca catcggatga taagtcaaat ttctcatcag tgccgaaaat ctcagggttt
      421 gcttggtaag cttgattgat tagattggca agataattag ccgaacgtat tcttgcttgg
      481 ctgtccattc cagtattgtc atcgtgtcct gcccaactac tgatagtgat agatggagtt
      541 gagacgatca gccatgaatc tcggtattca ttggtagaac cagtttttcc gacccaatct
      601 gcatttccta gatttccgtt taagcttgag atgacatctt tgaatggtgt agtgattttt
      661 tcattgatca cactacgcat catgtcattc atgatcgatg ccgttgcttc ggaataaata
      721 cggacagaat tactttcatg tttataaatc acattacctg cattatctgt gatggaatca
      781 atgatatagc cttcttcgta gactccacca tttgctaagg cctggaatcc atttgtctgt
      841 tgaagcgtcg ttacatctac tgtccctaaa ggagccgatt caacgcccca attatcattt
      901 gctgggtagt tcatcttcgc caagtagtgc tcgtaagcgt attgtttcga tccaccggaa
      961 ttcaacacat cttgatataa gtgataagct gggatattgt tggaccattc cagtgactca
     1021 cggacggttt ggaaggtgtt cgacccttca ttggtcgcat tgacgatttt ctcaccagca
     1081 ttttttcctt cttgccaagt cgttgcataa tctgatacac gggattctga tccgacaagt
     1141 cccatatcga ttgcaggacc gtaaacaaga accggtttga tggatgaacc agcttgacgt
     1201 tctgtatcaa aggcgtggtt attttgattc tggctgtaat tccgaccacc cacgaagcca
     1261 tagatacgtc cggttctgtt atccatcaat acatttccag tctccacttg gctaccagca
     1321 ccatcatcta acatgtagcc ataattttct acgccggttt gtaatgcagc atagatatct
     1381 ttatcgatcg tcgaatggat cgtatatcct ttgttttgga tcgtttgctg tgctttttga
     1441 taataagctt cactgaccga tgaatctgac atatcagcat tatctttttc tgccaattgt
     1501 tgtgcaataa tccttgttgc ttcgttcatc acggtataat acaagaattc tctgtcagtc
     1561 tgttccgcga tttgttgtgg gagaaagtct tttgtaagat cataggcttt cgcttcttcg
     1621 tattgttctt tcgtaatttg tccttcacgg tacatactga ataaaacaaa atcttttctg
     1681 gcaagaccgg cagaaagatc ttcttttagc gcgcctgtat tcgtgtatgg actatacgtg
     1741 atagggcttt gaggcaaacc agcgatataa gcagcttgtg gcagagtcac atcctttgca
     1801 tctacaccaa agattcctct agcagcttcc tggactccag caatattttg ccccttatta
     1861 ttccgaccaa atggtgagac attcaaatac gtagtaatga tctcattttt tgaaaagtgt
     1921 ttttctactt gtgcagaaag aacgatctcg tttgctttac gtttgaatgt tgtttcatcg
     1981 gttaggatct gttgtttgac taattgctgt gtcaaagtgg atcctccaga cgagccgatg
     2041 ccagtcgctt ctgaaactag tgctcgtaaa acagctttag ggacataacc ttgatgttta
     2101 tcgaagtatt catcttctgt actgataatc gcggttttca ataaaggcga aatcttatca
     2161 gatgaaatag tcgtccgcat cagatctgtc tggatttttg atatctctgt attatctgca
     2221 taaacaatct ttgacgtttc cgtaatgttt cctagttctg tctgcagttc ttttttagtc
     2281 ggcaattgtg tgtcttcgac aagataggca aagtagccag cacctattcc gatacctaga
     2341 gctgcgaaca acaaaagaag aacagtaata aaaacagtca atgattgaaa cactctaaaa
     2401 ataatcttag gaacaaacca tttttctttt tttcttctat tctgtttttt tcgagtattt
     2461 ttgcgtggca aaagggtcac ctcaactaat ggttttattt cgctaattat agcaaataat
     2521 aggacaaaaa aacagacaag gctgtttttg acaggaattt ctttttcgaa tgagataaaa
     2581 cgtgaggaaa tgatcttttt tatgaaagag ttcttaagct tttctttagg gaaagtttca
     2641 gttactatgg agaacagggc aaagaaaaac aaaccatttt ctttgtacat catcttatca
     2701 atatacagga attgttcgaa acaattgcct ttcaaggagg aatccatcca tgttattagg
     2761 ctcacatgtc agcatgagcg gaaagaaaat gctgttaggc gcggcagaag aagcagccag
     2821 ttacggatct tcgaccttta tgatctatac aggcgctcct caaaatacta gaagaaaagc
     2881 tatcgaagag atgaatattg atgccggaaa agcatttatg gcagaaaacg gactaacaaa
     2941 tatcgtcgtt catgcgcctt atatcattaa tttgggaaat acgatcaaac cagaaatgtt
     3001 tccatttgct gtacaatttc tgagagaaga gattgtccgt gcggaagctt taggtgctac
     3061 tcaaatcacc cttcacccag gcgctcatgt aggagcaggg gcagaagctg ggattgctag
     3121 aattattgag gggttgaatg aagttttgac aaaagaacag acggcacaga tcgctttgga
     3181 gacgatggca gggaaaggta cggaaatcgg tcgaacattt gaggaattag cgcaaatcat
     3241 cgatggagta aaattgaatg aaaaattatc cgttactttt gatacatgtc atacgaatga
     3301 tgccggatat aatataaaag aagatttttc aggtgtaatg gaagagtttg ataaggtcat
     3361 cggattagat cggcttaaag tactgcatat caatgattct aaaaatccac aaggttctca
     3421 taaagatcgg catgccaata tcggttttgg aacaattgga tttgacacat tgaacaaaat
     3481 cgtccatgat ccaatgtttg acaacatttc gaaaatatta gaaaccccat atgtcggtaa
     3541 agataaaaag aaagcttacg caccatatgg aaaagaaatt tccatgttca aagcgcaagc
     3601 attcgatccc gaagtgttcc ctgagttatt agcagaagaa gtcacacttt aagttttcta
     3661 cctgacagca acaaaaaagg attgggcagt ctgccttgtc cttttttgtt taagaaacaa
     3721 aggagtgctt tttgcattcg ttttcgccat gctccttttg ccccctcttt ttaaattcag
     3781 acaaacaggg tatactgata gaaaagtaaa aaacgaaaag tttcacaaac aagtagcaaa
     3841 acgtgattga aaggacgaaa agatgctgaa cattttaaac cccttgatcg accaactaaa
     3901 aaagaaacat acatacttta ttcttggcat agccgtatta tttacaatta ttcggttgta
     3961 tttatatagt aaagcaatct atggaacaga tatgtcggcg acctatgatg atcagctgct
     4021 gattcactat gctcaaaata ttttggatgg taaatggcta ggggagtata cgactacaac
     4081 actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc
     4141 ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt
     4201 taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt ttttgttttc
     4261 tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc tcgtgattcc
     4321 ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat acggttctag
     4381 cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat
     4441 tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt
     4501 ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact
     4561 ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg
     4621 cttttccatc gtctatcagt ggatcgaata taaaaatgag acaacatacg gtattgcaac
     4681 catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga
     4741 tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa
     4801 taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata
     4861 tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt
     4921 ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac
     4981 caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaaccat
     5041 caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct
     5101 gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt
     5161 ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt
     5221 attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt
     5281 ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc
     5341 tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt
     5401 tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact
     5461 gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc cagaaaaaaa
     5521 ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat tcatcgtagt
     5581 aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa
     5641 agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag
     5701 tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt tgtttccttt
     5761 atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga ttacataagc
     5821 gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat
     5881 ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga accagccaag
     5941 aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat
     6001 catcagtaaa ggaatcatcg aaaatggcaa cgcaaaagcc aagaacacct gggattcatt
     6061 cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg taaacaaaac
     6121 acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg
     6181 gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt
     6241 agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt
     6301 tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag cttcatacag
     6361 accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac ccatgatcaa
     6421 tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc aagtagaaaa
     6481 acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag tttgtgaaac
     6541 tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc ctaaagctcc
     6601 agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac tgttagggat
     6661 aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac
     6721 gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa acccgacttt
     6781 tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg
     6841 aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcga tgtctgttgc
     6901 cataatggct aattcagtca agatccataa aatgacaccc aatgtcttac ttgtccgtgc
     6961 tcggattgct tgggccaaat ccatttgggt cacgattcct aattttgctg ccatatactg
     7021 taaaagcata gcaattagac tagacatcaa aataactgac atcaataaat attggaaatt
     7081 ttgtccacca gtgatcgaag tggaccagtt acctggatcc atataaccaa ctgctactaa
     7141 tgcacctggt ccagagtaag caagaagtgt tttaaaaaaa ctttttccct ttggtacatc
     7201 cactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt
     7261 atgaaattgt ttgttggatt gattattcaa gaaaaaacca cctttcctat ttatctacga
     7321 ttacattcta cacctcagaa agaaaaaatt aaagaaaaag attcggtatg cctaattttt
     7381 tttcgtggtg tgtcttgctg gttaatgaaa gaaattttgt tcttctctaa aaaaaccact
     7441 attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa
     7501 atcattgaga gcaattcgga aatagaaagg agagatttat atggtatcaa caggtattgt
     7561 cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg
     7621 taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc
     7681 aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca
     7741 aatgaagaac caagggaaaa aataggttct gacccaaaga ctttttgcgg gctgattttt
     7801 tcagtccgtt ttttatttcg taatttgtta gactattcag actattcagc aaaaaagaca
     7861 agcacagaca atcctatgct cttgataaag acaaaggagg agtaaaatgt cgatttttag
     7921 aaaattaggt tggttttttc gtcaggaaaa gaaacattat ttaattggga ttttctccct
     7981 attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca tcattgatga
     8041 gatcgccgat aagaatttat caataaagat catttcctgg tggatcttat tattgatcct
     8101 tgcagctttc ttacaatatc tttttcgtta tatctggcgg acgaatattt ggggaagtgc
     8161 agctcggttg gagagagaac ttcggcagca gctattcaaa cattttacag aaatggacaa
     8221 tctcttttac caaaaatatc ggactggaga tttgatggct catgcaacga atgatttgaa
     8281 tgcaatccag aatgtagcag gagctggaat cttgaccttt gcagattcag tgattacggg
     8341 cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac tgattgcgct
     8401 gattcctctg cctttgctag ccgttacttc acgagtgctg gggtcaaaac tccacgatgc
     8461 ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag aaagtatcac
     8521 tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctcgcgg attttgaaga
     8581 aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact ccttatttga
     8641 tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt taggtggaaa
     8701 gtatgtcttg gaaggcacga tctcaatcgg gcagatggtc tcatttatta gctatatcgg
     8761 tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg aaagaggaaa
     8821 tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg tggaacaact
     8881 gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct cttttaacta
     8941 tccgaaagaa gaatcaccag cattagaaca aatcaaattt gcgatccaag aaggagaaac
     9001 attaggtatt gttgggaaaa ctggtgcggg aaaaacgaca atcatcaaat tactgatgcg
     9061 tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca aagaatattc
     9121 attgaatgct ttattaggat caatcggtta tgtgcctcag gatcacttcc tgttttcgat
     9181 gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag aagtagagca
     9241 tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttgccaaaag gttatgatac
     9301 cttagttgga gaacgtggag tctctttatc tggcggacag aaacagagga tttcgattgc
     9361 tcgagcattg atcaaaaatc ctgaattgct gatcttagat gatgcacttt ctgcagtaga
     9421 tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag agaaaacaac
     9481 catcattgca gctcaccgtt taagcagcgt gatgcatgca aaagaaatca ttgttgtcga
     9541 tcaagggaaa atcattgaac gaggaaccca ccacgagctg cttgcattag gtggctggta
     9601 tgaacgaatg tgggaaaaac agcaattaga agcgaagata gaaggaggcg aggcctgatg
     9661 gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca gaagaagatc
     9721 atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt tgctatctta
     9781 tttgcgtttg ccctgtctgt gatcaatgtc ctattgccaa ggatcatcca aacttttatg
     9841 gatgatcatc tggctaaaca gtcagctacg acacaagtta ttttgttttt tgctggtgtg
     9901 tacttgtttg gagtcatagt caaaagtatc atttggtttt tccaatggta tttgtattcc
     9961 atggcttcat tgaagaccta tcagtatgtt cgggtaagat tatttgaaaa gctgcataca
    10021 ctaggtatgc gttattttga ccagacaccg gcaggatcga ccgtttctcg agtgacgaat
    10081 gacacagaaa cgctatttga attttggtat gtctttttga tggtattgac tgggatcttt
    10141 gcagtcgttt cttctttcgt tgccatgttt caaatcaatg gagaaattgc cttgtggact
    10201 ttggtgttct tgccgatttt aggaatcgtc atctggtatt accagaaatt cagttctcgt
    10261 atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa tgaatcgatt
    10321 tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa agaatttgaa
    10381 caagtgaaca acgagtattt agacacgcgc tatgcgatga tcaaaacgaa ttcgattctg
    10441 ttaagtccaa tcatcaattt tctctatgca ttggcgattg cgttggcgtt gactttgttt
    10501 gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt tacgacctat
    10561 gtccaggaat tttttaatcc aatgtctcaa atgatggact ttttgagcac ctttacagat
    10621 gggatggttg ccggtagtcg tattttgaaa atcatggata cagaagaata tgcaccgcaa
    10681 caaaatcctg gagcagatca agcgattact tcaggtaaga tcgagtttcg caatgtaagc
    10741 ttttcttatg atggagagca caatgtattg aacaatatct cttttgtcgc taatccgggt
    10801 gagaccgttg cattagtggg gcacaccgga agcggaaaaa gctcgatcat caacgtattg
    10861 atgcggtttt acgaatttta tgaaggccaa atcttgattg atgatcatga cattcgagaa
    10921 tatccaatgg aagagttacg taagaaaatg ggattggtct tgcaagatgc cttcttgttt
    10981 tatggagata ttgcaggaaa tatccggatg atgaatccag ctatcacaga cgaacagatt
    11041 cgccatgcag ctgagtttgt tcaagcaaac aagttcatcg atgaattacc aaaaggatat
    11101 cactcaaaag tgatcgaaca cggtgccagc tattctagtg gacaacgaca gctgatcact
    11161 tttgcgcgaa caatcgtgac agatcctaaa atactagtat tggatgaagc aacagcaaat
    11221 atcgatactg aaaccgaagg attgatccaa gaaggattgg caaaaatgcg acaaggacgg
    11281 acaacgatag cgattgccca tcggctgtcc acgattcggg atgcagaatt gattttagtt
    11341 ttagacaaag gtcatatagt agagcgtggg aatcatgaaa cacttctagc gcaaaatggt
    11401 ttgtatgcag atatgtacaa actgcaaagc agtggtgtgt aaaaagaggt tgtgacagta
    11461 gcatagtagc attttgccct aagcgctaga gtgaaaataa aacagagctt cgcagattct
    11521 tcatctgtga gcgttagtgg cgtagtcacc cgatttaaaa aagacgacga cagctagtca
    11581 aaagcggcta ataaccacta agaaagcgtt gacaaaagcg tttgctttcg ataatataaa
    11641 gaaaacgata caacttggat agtgattgcc tgatgcaagc taaacctgat gagtctccca
    11701 tggttccatg gacggctttt tgggtttttc tttttgtttt ggatacagcc ctatatatca
    11761 tggaaaggag caaaacatgc tgattgagaa agtactgaat aacaacgtcg taataaccag
    11821 ggatgaaaga aatcaggaaa tgatcgtgat gggaaaaggt ttggcattca aaaagaaagt
    11881 gggtgacccg atttttgact catgcatcga caaaaaattt cagttgacaa atgacgggct
    11941 ttcacaacag ttccaagaat tggcacaagc gattcctctt gagtacttag aaatttcttg
    12001 tgaaatcatt gaatatacaa aagaggctct acaagtccag ttgaatgatt ctgtgtatat
    12061 tgcattgacg gatcatttgt atacggctat cgaacgtcac aaaatgggaa tatttgtacc
    12121 aaatgtattg ctgtatgata tcaaacaact atttcctaaa gagtatgcca tcgggaaaaa
    12181 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    12241 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccaacaagac aatacctacc
    12301 tgttcactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    12361 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    12421 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    12481 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    12541 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    12601 gtttagtgca tgagaatcgt tgtaaataaa taaaaaaagg aggataaatt aacaagaacg
    12661 tatgaaataa gggtagtgac attcagttgg ctaaaccttc cactaataaa ttggagggaa
    12721 tagaaatggg aaaataccaa gaattagcga gaaaaatcgt agaaaatgtc ggcgggaagg
    12781 aaaatatcaa tggtttgaca cattgtatca caaggcttcg tttcaaatta aaaaacgaag
    12841 aaaaagcaaa tgatgagatc ttgaaaaata tggacggtat tgtaacggta atgcgagcgg
    12901 gagggcagta tcaagttgtc atcgggaatc atgtgccagt cgtgtttgaa gaggttataa
    12961 aagcaggaaa ccttactttt gatgaggctg tttcaacaga aaaaatgaga ccgtttgata
    13021 tgcttattga tggaatatcg ggctgtttcc aaccattcct tgctatccta gctgctgacg
    13081 ggatgattcg tggactgaca gcattccttg tcttcttagg cgcgtttgac cgcggttctg
    13141 gcacatttgt tatgtttgat aatatcggtg attctgtatt ccaatttatg cctgttatta
    13201 tcggtttaac agctgcccgt aaatttaaag taaatgaatt cgtagggatg ttgcttggcg
    13261 cagcattaat gaatcctagc ttgtcgctgg aagctttgag tggtgcagca gaagcaccac
    13321 taacgaccat attttctggc actatttttg aagcacctat ttatcaaaca gtattcggca
    13381 ttccctggat tgcgcgtaac tacgcttctt ctgtcattcc cattatcttt attgtgttgt
    13441 tagctagcca aattcagaaa ccaattaaaa agttagtacc tgagatgatc gcgaatttct
    13501 tcgttccatt cttcaccgtt cttattacaa tgcctttagg ttttttactc gttggaccag
    13561 tattcacatt cgcaactgat attttaatgg ctggatttga aacactttta gcactttcgc
    13621 cagttattta tggagcaatc gtaggcttct tctggcaaat tctggtgatg tttggtctcc
    13681 attgggcaat cgtaccaatg ggattgatgc aattttctgt gaatggttgg caaaatatta
    13741 tgacacctgt tgcagtagtg tcttttggac aaactgcagc acttaccgca ctctacttta
    13801 agcttagaaa tccaaaagac aaagcaattg caataccagc cattgtttct ggtattgtgg
    13861 ggattacaga gccagctatt tatgggttta cattgccacg taaaaaaata tttattttta
    13921 cctgtgtcgg tggtgcgtta gggggcgcgt atagtgggtt aatgaattta acttcttgga
    13981 atcaaggtgg actaggcatc tttacaattc ccaactatat tagaccagat ggtgatttaa
    14041 cagatgtgat taatgtattg attgggatcg ccattgcgat ggtggtatcc tttgcgctga
    14101 cttttttctt ttggaaggag gaagcagatg aaacagatga tagacagaaa agatcaggta
    14161 aagaaattgt aaaaacaccg atacaaggac aaatcgctcc actgaatgca gcaaaggatg
    14221 cagcttttgc acaaggaaca ctaggtcgag gaatcttgat ctaccctgaa aaaggagaag
    14281 tacgtgcccc ttttgacgga acagtcatgt ccctgtttcc gacgaaacat gcaatcggga
    14341 tggtttctga agcaggtctg gaattgctga ttcatgtcgg attggatact gttcagttgg
    14401 aagggaaata ttttgaaagc ttggtgcagc aaggagaaag agtgaaacaa ggcgatctgt
    14461 tgcttcgctt cgacaaaaag aaaatcgaag aggaaggata cagtttagaa acacctgtga
    14521 ttgtcatcaa ttacatggat tatttggata ttttggaaaa tcaaggggaa acggtcgggc
    14581 cagacgatgc acttatcacc gcactgacct gaacggttag gtgaaagaat ggactaggct
    14641 taactattct ttttagtcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc
    14701 tgagctgata gggcgtttat caaatttttg tcctacagaa attagatgga ctaaattctg
    14761 ttgataattt tctgcaaaaa cacaactata taacacgtct agaagataac aaatcgatgt
    14821 attgatcgta aaattaccaa ttttcgaata cagtcgttcg cgtgtcgtaa gtcgcaaagc
    14881 ggctgtactc attgaagata gcgttgaatc accgatactg gtcaaagcta aaattggcac
    14941 atgattttgt ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga
    15001 aatcagaatc ccacacatat cacttgtgca attgtacgct tcatagaacg attcaccaaa
    15061 agtggtgaca atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga
    15121 aatcaaagta ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg
    15181 tttggctttt tgcagttggt catgatgaat gagagaaagt gtatcttgaa tcgtcatctg
    15241 ttctaaagaa gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag
    15301 attcgcatct acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt
    15361 acgaaaactg acccagccat caaaacccat tttttttgct acacggatga gtgtggacgg
    15421 gtggacataa catcgctcag caatctcttg gatggtcatt tcgccaagtg ctggagaatc
    15481 gcttaagaga aactgaatca cttcattttc tgaaggagaa aaggagattt ctttgatttt
    15541 ttcacgtaat aacatgtaga taccttcttc ttgttttttt acgtaaatca taactttttt
    15601 gaaaatgatt tgtaaacgga tttatgttat atagcttaat aaagacattt tttgacaaga
    15661 tatttacaaa aatccagaag aaaacactaa cttttgtaaa tgttactgga aagaaacgaa
    15721 tttctattgt ttgttttgtt tacaaaaaag ataataaaag tgtaaaaagt aaaggagtga
    15781 gaacatggca aaaggaatca aaatcgctac gattggtgga ggcagcagtt acacccccga
    15841 gttgatggaa ggatttatca aaagatatga ggaattaccg atacgagaaa tctggctcgt
    15901 agatgtagaa gaaggaaaag aaaagcttga gatcgtcagt cagatgtctc agcgtatgtg
    15961 ggatgcttct ccgtatgaag tcaaaatcta tgcaactctg gatcgggaag aagcattgaa
    16021 agatgcggat tttgttacga ctcagtttcg tgtcggtcaa ttagatgcac gaatcaaaga
    16081 cgagcggatc cctagttatt atgggatgat tgggcaagaa acaaatggtg ctggcgggat
    16141 attcaaagct tttcgtaccg ttccagtggt tttggcaatc gttgaagaca tgaagcgttt
    16201 atgtccaaat gcttggctga ttaatttcgc gaacccaagc gggatggtga cagaagcagt
    16261 tatcagatat ggcaaatggg ataaagtgat cggattatgc aatgtgccag taatggcgat
    16321 gatgacagaa ccaacaatgc ttggcaagaa aaaagaagag ctgatctaca agtttgccgg
    16381 tctaaatcac ttccattggc acaaagtagc agataataaa ggaaatgacc gcacggcaga
    16441 attgattgat cgtttatacg atgaagacaa tggattgccg aaaaatatct ttgacatccc
    16501 gtttttcaaa gaacaattaa aacaaatgaa catgatccct tgtggctacc accgctatta
    16561 ctatcgtgaa gaagaaatgc tggaacatgc tttagaagaa tatcaaacga ttggtacaag
    16621 agcgcagcaa gtgaaagaaa cagaagcaga gctgtttgaa ttatacaaag atccagaatt
    16681 ggatcataaa cctgaacaat tgcaaaaacg aggtggtgct tattattcag atgctgcctg
    16741 tgaaacgatt gcttcgatct atggaaataa agaaacacag atcgtggtat ctacaaagaa
    16801 tgatggagct gttcctgatc tgccggcaga ttgtgtagta gaagtcactg cctatgttgg
    16861 cgcacaaggc gcacgaaatg tagcgtttgg ttcccttcct gctgctgaaa gaggctggtt
    16921 gcaagtaatg aagaatatgg aattgctgac gatcgaagca gccgtgactg gtgattatgg
    16981 aacagctttg caagcgttta cgatcaatcc gcttatacga tcaggaaaga cagcaaaacg
    17041 gatcatggat gagttgttta ttgcacatca gcagtattta ccgcaattca aggaaacaat
    17101 cagccggtta aaagcagaag gaatcgaagt ccaagatgaa ctggcaagag aattatcaag
    17161 caatacacat gtaatggagg aagtatgatg ggattcagaa aagatttttt atggggcgga
    17221 gctacagcag ccaatcaatg cgagggcggt tatgatgaag gcggacgtgg gttagcgaac
    17281 gtagatgttg tgccaatcgg agaagaccgt tttcctgtca ttactggaaa aaagaaaatg
    17341 tttgaatttg atgaaaatca tttttatcca gcaaaaattg cgatcgatat gtatcatcat
    17401 tataaggaag atattgcttt atttgcagaa atggggttca agacttatcg gatgtccatt
    17461 gcttggagtc gtatttttcc aatgggcgat gaaaaagagc caaatgaatc tgggttgaaa
    17521 ttttatgagg atgtctttaa agaatgtcaa aagtacggga tcgagccgct tgtgacgatt
    17581 acacattttg attgtccgat gcatttgatc aaagaatatg gtgcgtggcg ttccagagaa
    17641 ctcgttggtt tttatgaaaa tctgtgccga gtcatcttca atcgctataa aggacttgtc
    17701 aaatattggt tgacattcaa tgagatcaat atgatccttc atgcaccatt catgggcgca
    17761 gggctgtatt ttgaagaagg agaaaatgaa gaacaaatca aatatcaagc ggcacatcat
    17821 gagttggttg caagtgccat cgcaacgaag atcgctcatg aagtagatcc ggagaacaaa
    17881 gtaggctgta tgctggcagc aggagcatat tatccataca cttgcaatcc tgaagatgtt
    17941 tgggcttcta gaaagtcaga tcgagaaaat tatttcttca ttgatgtcca gtcacgagga
    18001 gaatatcccg cttatgcatt gaaagaaatg gaacgaaatg gcattcagat caaaatggaa
    18061 cctggggatg ccgcattgct gaaagaacat actgttgatt ttatctcctt ttcttattat
    18121 tcttctcgag tttcgactac tgacaacgaa ctcttggaac agactgcagg aaatatcttt
    18181 gcttctgtca aaaatccata tctggaagca agtgaatggg gttggcaaat tgatccatta
    18241 ggcttgagaa taacgatgaa tgatatttat gatcgttatc aaaaaccact atttattgta
    18301 gaaaacggct taggtgcagt cgatacgccc gatgagaatg gttatgtgga agatgattat
    18361 cgaatcgact atctggcagc acatatccag gcaatgaaag atgcagtaga attagatgga
    18421 gtcgatctat taggctatac gacttgggga tgtattgatt tagtatcagc tggtacaggt
    18481 gagatgaaga aaaggtacgg attcatttat gtcgatcgtg acaacgaagg aaatggcaca
    18541 ttagcccgtt cgaagaagaa atcattcaac tggtataaaa aagtaattga ttcaaatgga
    18601 gaaaatcttt cgaacgattg atcaatgatt tataaaagtc tattgttttt cagcggattt
    18661 ttcgagtata ataaagctat tcgagaaacg caagcagaac aatagacttt ttttcgcatt
    18721 ggctgtaaag tatgcttact tgattaagta aaggagaatc tacatatgta tgatgtaatc
    18781 gttattggcg cagggcctgc tgggatgact gctgctttat atgcttcacg ttcaaactta
    18841 tccgtattga tgatcgaaca aggcgctcct ggcggccaaa tgaacaatac ggcagaaatc
    18901 gaaaactatc ctggtttcga ttcaatcatg ggtcctgatc tagcttataa gatgtatgaa
    18961 ggtgtttcaa gatttgggac agaaaatgct tatggaatcg tgcaagatat caaggatcac
    19021 ggcagccaca aagaagttat ttgtgaagat aaaacttacg aaggaaaaac agtcattatc
    19081 gcaacaggct gtatccaccg taaattaggc gtacctggcg aggaagaatt tgctggacgt
    19141 ggcgtttctt actgtgcagt ttgtgatggt gcatttttcc gtaacaaacg tctagtagtg
    19201 atcggaggag gagactcagc agtagaagaa gccatctatt tgacacaatt tgcttctgaa
    19261 gtagtcatcg tccaccgtag agatgaatta cgtgcacaaa agatcatcca agaccgtgca
    19321 tttgcgaatg aaaaaatttc ctttatgtgg gatacagtcg ttgacgaaat cgttggaaat
    19381 gaaatggtag tgacaggtgt gaaagcacgt aatgtcaaaa ctggcaatga gtttgatttg
    19441 gcagcagacg gagtattcat ctatgtagga ttggatccat taactgcacc atttagagaa
    19501 gctgggttga cgaacgaagc cggctggatt ccaacagaca acgaaatgaa gacgaatata
    19561 ccaggcgttt ttgccatcgg agatgttcgt gaaaaagatt tgcgacaaat cactactgct
    19621 gttggtgatg gtgggattgc tggacaacaa gtgtataaat acttggaatc tttagaagaa
    19681 acaactgcat aagtttaaaa agagtgaaag aacggtttca attgttcttt tactcttttt
    19741 tttgcgtgaa atctactgaa tccgatttat gcgaatactt gtaaccgtgc ttgataaaag
    19801 aatcttttct tgatttaagc gttttttggt cctcatactt tcatatctaa taagtgaatg
    19861 ctatacttat taagaaccat atttcagaaa gaggtgttat tttcatgtct tgggaacaag
    19921 tgtatgaaca atgggtacat gaagaaaatt tagaagaaaa cttaaaaaaa caattaaatg
    19981 atctgggtga agatcctgaa aaacttgaag atgcttttta tgcaccgttg gaattcggta
    20041 ctgcaggcat gcggggaatc ttaggacctg ggatcaatcg aatgaacatc tatacggtaa
    20101 gacaagcaac agaaggactt gcacgtttca tggatacaca aggtgaagaa acaaaacgcc
    20161 gaggtgtcgc tatcgcttat gactcacgcc atcaatcacc agaatttgcc atggaagcag
    20221 ccaaaacatt agcacatcac aatatccctt cttatgtatt cgaaagttta cgtccaacac
    20281 cagagctttc ttttgcagtc cgtcatttga agacgtttgc gggaatcatg gtaactgcat
    20341 cccacaatcc agcagcttac aacggatata aagtctacgg agaagatggg ggacaaatgc
    20401 ctcctgcaga cgcagatgca ttgacgaaat atgtccgtga agtcagcaat ccgttaaaag
    20461 ttgaagtatt atctgacgac gaagctgaac atagcggatt gatcacgatc atcggagaag
    20521 aagtggatgc cgcttatcta gaagaaatca aagcagtaac gattgatcat gaattggtgg
    20581 aaacaatggg taaagatttg aagcttgtgt acacaccact tcacggaaca ggaaaaatgt
    20641 tgggcgaacg tgcgttgaaa caagctggat tcgaacaatt cgtactagtt ccagaacaag
    20701 ctgtcgcaga cccggatttc actacagtca aatcaccgaa tccagaagaa cactctgctt
    20761 tcgagtatgc gatgcgactt ggcgagaaag aaggcgctga cttactgatt gcaacggatc
    20821 ctgatgcaga ccgtttaggc gcagccgttc gtttgccaaa tggctcctat gaagtattga
    20881 caggaaacca aatcggagcc ctgtttgctc aatatatttt ggaagcacac caatcgcgag
    20941 gcactttacc tgaaaatgca gcggtattga aatcaatcgt ttctagcgaa ttgcctacag
    21001 caattgcgga acactatggg gctaccatgt tcaatgtatt gacaggattc aaattcattg
    21061 ctgaaaaaat tcaacaattt gaagaagatc attcccatac ttttatgttc ggattcgaag
    21121 aaagttacgg ttatttagtt aaacctttcg ttcgcgacaa agacgcgatt caagcattgc
    21181 tcttattagc agaagtagca gcttactata aaaaacaagg caaaacattg tatgacggct
    21241 tgcaaacgat ttttgaaaca tatggctact acgcagaaaa aacgatttct gtgacaatga
    21301 gcggacaaga aggctctgca aaaatctctg ctttgatgga aaaattccgt gaagaagcac
    21361 cgacagaatt tgccggagca aaagtcatcc aaacagaaga cttcaaagag ttgacgagaa
    21421 cagaccgtga aggaaacgta gaaaaactaa cgacaccgcc atctgacgta ttgaagtata
    21481 cgctggaaga aaatggctgg atcgctatcc gtccttcagg aactgagccg aaaatcaaat
    21541 tttatatcgg tgtaaaagga aactcttcag aagacgcagc tgaaaaagta gccgcttatg
    21601 aagcgattat caaaaaaatg acagaagaat aaaaaaactt gagactgatg gaaaaagtgg
    21661 taaactttag aagtcagtct tttttcaagt tggaggtgaa tggattgcca gacacgcaaa
    21721 aaacaaaagt agagaaagtc agccaactat tcaaaatgtt gagtgacccc actcgtctca
    21781 aaattctgct gtatctgaaa gacggggaac aaaatgtgac agcaatcacc caagcagtag
    21841 aaatggaaca atctgctgta tcgcatcaac tgcgtttatt aagagaaaac catgtcgtca
    21901 agtcccatcg tgagggaaaa gcgattttgt atagtcttga tgattctcac gtactggata
    21961 ttttgaacca gacgctcaaa catgtggaac atcaataacg ataggtgcaa atagagacaa
    22021 actggactgt atcaacgaat cttcttcgct gacacagtcc agttttttta agaaaaagac
    22081 aagaaaaact gttagtctgt ttcttcggtg gtttcagttc cataataact atgatcttct
    22141 aggtctaaag cattcaaaat aagtgaagct gtttcttcga tagataaaga agcgacattg
    22201 attactacgc agcccaattt ttcataaaga tcattcgcaa aatcgagttc agctcgtatt
    22261 ttttcgatat cggaataagc agtatcagga tttaatccgt aagagcgcat ccgttcttta
    22321 cggatattgt ttaaaatatc tgggttattc gtcagtccca cgattttttt aggatcgatc
    22381 tcccaaagct gtttgggaat atgtgcttgt gggatcaaag gtagattggc aacctttaaa
    22441 tttttgttag ctaaaaacaa gctcaaaggc gttttggaag ttctggatac gcctagcaaa
    22501 acgacatctg cttctagaaa cccacgagga tctttcccgt catcgtattt tacagcaaat
    22561 tccattgctt tgatccgttt gaaatagttc tcattcaata aatgaagggc gccaggttgc
    22621 cgaatgggct tgataccagt taagcgctcg atttctgata tcggcggagt catcacatca
    22681 aaactaaata attgatgttc ttcacaaaaa gaacgcgtca tctcgatcaa ttcttgattg
    22741 atcaatgtgt gtaggacaag ggctttttct tttagtgcat cttgcagtgc ctgcatcaat
    22801 gttttctcat ctgtcacaaa tgttctgcgg aataaattga actcaacggt aggatattgg
    22861 gccatagtgg cttgcgctaa ttttgtcgca gtttctccag cagagtcaga gatgacaaaa
    22921 aaagtaatga tttctgtaga atcggaagac atggataaaa cctcacttgc atttttttta
    22981 tgtttattat accatactct agaatgtgta cttcgtaatc attttgattt ggaggaataa
    23041 gtcatgaaaa aacagacatt gattgaacaa tcactagaaa cattgaaaaa taacggtttt
    23101 aaatatacta aaaaaagaga agcattgtta acttatttag tgaagcgcaa tcgctacgta
    23161 tccgctaaag aagtttttga ctatatgaat caggaattca aaggagtaag ttacgatacg
    23221 atttatcgaa atctccatga ttttgcagaa atcgatttac tggaagaaac cgatttaaat
    23281 ggagagatga agtttcgttt tcattgctgt caaggggaaa tcggccatca ccatcacttt
    23341 atctgtacgg tgtgcggaac aacgaaagaa atccatatgt gtccaatgaa ttatttcgaa
    23401 gaacagctcg gtggatgtac gatcgaaggg catagattcg agattttcgg aagatgtgaa
    23461 aattgtcaat aaggatttaa ataaaataac atttgataat tttaaaaaaa agcaagattt
    23521 ccttgacggt caaaacagca ttagatataa tatttacagg acagtcttgt tttttgagat
    23581 atatttcaat aacaattacg gttacttttt aagctcggag ggagggaatt aacatgtcaa
    23641 aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct tcgtcgcttc aaacgttccg
    23701 tgtcaaaagc gggtacttta caagaatctc gtaaacgtga attctacgaa aaaccaagtg
    23761 taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa aaaattctag ttctgtgatg
    23821 aattggagtt ggtagtgtgt cactactaag tagattaaac gatgacatga aaacagcgat
    23881 gaaagcgaaa gataaggaat ctttacaagt catccgaatg atcaagtcat ctatacaaaa
    23941 cgagcagatt aaagaaggtc acgacttgac agaagaagaa gaattaacgg tgttgtctcg
    24001 cgaaatgaaa cagcgacgtg attctttgca tgagtttgaa gaagcaggac gtgacgattt
    24061 agctgaaaaa gtaaaaagtg aaattgtgat agtcgaaaaa tacatgccgg aacaactttc
    24121 cgacgaagaa attcgacaac ttgttcaaga agcaatcaca caaactggtg cttcatcaat
    24181 gaaagaattt ggcaaagtca tgggtgcgat catgcctaaa gtcaaaggaa aagcagatgg
    24241 aaaccaagta aatgccattg tgaaagaact attacaatct taattttatc atttaaaggc
    24301 tcttcgtcaa tgagtgttgg tgaagaaatt c
//
LOCUS       DAZKOK010000029        24273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000029 DAZKOK010000000
VERSION     DAZKOK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.029"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG90W_001058"
     CDS             complement(54..788)
                     /locus_tag="ACG90W_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0477436.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ACG90W_001059"
     CDS             1115..1525
                     /locus_tag="ACG90W_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0477437.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ACG90W_001060"
     CDS             1518..2219
                     /locus_tag="ACG90W_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0477438.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ACG90W_001061"
     CDS             2219..3805
                     /locus_tag="ACG90W_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477439.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ACG90W_001062"
     CDS             3824..4045
                     /locus_tag="ACG90W_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0477440.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ACG90W_001063"
     CDS             4042..4803
                     /locus_tag="ACG90W_001063"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0477441.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ACG90W_001064"
     CDS             5183..5692
                     /locus_tag="ACG90W_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0477442.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ACG90W_001065"
     CDS             complement(5767..6306)
                     /locus_tag="ACG90W_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0477443.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG90W_001066"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ACG90W_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0477444.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ACG90W_001067"
     CDS             7319..8170
                     /locus_tag="ACG90W_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0477445.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ACG90W_001068"
     CDS             8198..8833
                     /locus_tag="ACG90W_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0477446.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ACG90W_001069"
     CDS             8853..9626
                     /locus_tag="ACG90W_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0477447.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ACG90W_001070"
     CDS             10034..10831
                     /locus_tag="ACG90W_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0477448.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ACG90W_001071"
     CDS             10809..11222
                     /locus_tag="ACG90W_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0477449.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ACG90W_001072"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ACG90W_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0477450.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ACG90W_001073"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ACG90W_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0477451.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="ACG90W_001074"
     CDS             15999..16559
                     /locus_tag="ACG90W_001074"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0477452.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG90W_001075"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ACG90W_001075"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0477453.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ACG90W_001076"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ACG90W_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0477454.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ACG90W_001077"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ACG90W_001077"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0477455.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG90W_001078"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ACG90W_001078"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0477456.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG90W_001079"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ACG90W_001079"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0477457.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ACG90W_001080"
     CDS             21906..22484
                     /locus_tag="ACG90W_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0477458.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ACG90W_001081"
     CDS             22536..23018
                     /locus_tag="ACG90W_001081"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0477459.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ACG90W_001082"
     CDS             23135..23509
                     /locus_tag="ACG90W_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477460.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23884..>24273)
                     /locus_tag="ACG90W_001083"
     CDS             complement(23884..>24273)
                     /locus_tag="ACG90W_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0477461.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7906 a         4353 c         5197 g         6817 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tatttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcacagcat gacagcattg
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc actagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgggagcgtc aataattttg tgtaaataaa ttgtcctcct gcaaaataat
    23881 tagttactca gtaaacattg aaactaatgt atcggttacc tgttgaaaac ctttatggct
    23941 tctgtttaga aatttttgat tgtatgtatc aaaaatgctg actagaaagc gttctagtga
    24001 ttcttcattt tgaaactgct cttttctacg gctgtatctt ttaatttgct tattgaaaga
    24061 ctcgattaga ttggttgagt aaatggttct acgaatgcta ggtggaaaat cataaaaagt
    24121 taataagtct tggttttcta tgagtgactg cgtcacttta ggatagtttt tcttccattt
    24181 ctcaatcatg ccggataaga aggtattcgc ttcttctttt gagttagctt gataaacagc
    24241 cttaaagtca tcacagattt cttttcggtc ttt
//
LOCUS       DAZKOK010000030        24258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000030 DAZKOK010000000
VERSION     DAZKOK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.030"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG90W_001084"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG90W_001084"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0477462.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG90W_001085"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG90W_001085"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0477463.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG90W_001086"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG90W_001086"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0477464.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACG90W_001087"
     CDS             complement(3374..4681)
                     /locus_tag="ACG90W_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0477465.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACG90W_001088"
     CDS             complement(4684..5265)
                     /locus_tag="ACG90W_001088"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0477466.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACG90W_001089"
     CDS             complement(5837..7129)
                     /locus_tag="ACG90W_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0477467.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACG90W_001090"
     CDS             complement(7126..7749)
                     /locus_tag="ACG90W_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477468.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACG90W_001091"
     CDS             complement(7752..8588)
                     /locus_tag="ACG90W_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0477469.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACG90W_001092"
     CDS             complement(8605..9492)
                     /locus_tag="ACG90W_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0477470.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACG90W_001093"
     CDS             complement(9508..10791)
                     /locus_tag="ACG90W_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0477471.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACG90W_001094"
     CDS             complement(11474..12157)
                     /locus_tag="ACG90W_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0477472.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACG90W_001095"
     CDS             complement(12175..12792)
                     /locus_tag="ACG90W_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0477473.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACG90W_001096"
     CDS             complement(12802..13626)
                     /locus_tag="ACG90W_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0477474.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACG90W_001097"
     CDS             complement(13619..14179)
                     /locus_tag="ACG90W_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477475.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACG90W_001098"
     CDS             14353..15309
                     /locus_tag="ACG90W_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0477476.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACG90W_001099"
     CDS             complement(15614..16570)
                     /locus_tag="ACG90W_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0477477.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACG90W_001100"
     CDS             16674..17276
                     /locus_tag="ACG90W_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0477478.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACG90W_001101"
     CDS             complement(17364..18863)
                     /locus_tag="ACG90W_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0477479.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACG90W_001102"
     CDS             complement(18879..19403)
                     /locus_tag="ACG90W_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0477480.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACG90W_001103"
     CDS             19701..20450
                     /locus_tag="ACG90W_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0477481.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACG90W_001104"
     CDS             20441..21226
                     /locus_tag="ACG90W_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0477482.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACG90W_001105"
     CDS             21195..21911
                     /locus_tag="ACG90W_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0477483.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACG90W_001106"
     CDS             complement(22013..23032)
                     /locus_tag="ACG90W_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0477484.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24052)
                     /locus_tag="ACG90W_001107"
     CDS             complement(23051..24052)
                     /locus_tag="ACG90W_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0477485.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7189 a         5222 c         4224 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcgactga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAZKOK010000031        24122 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000031 DAZKOK010000000
VERSION     DAZKOK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.031"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            432..596
                     /locus_tag="ACG90W_001108"
     CDS             432..596
                     /locus_tag="ACG90W_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477486.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            593..823
                     /locus_tag="ACG90W_001109"
     CDS             593..823
                     /locus_tag="ACG90W_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477487.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            1008..1553
                     /locus_tag="ACG90W_001110"
     CDS             1008..1553
                     /locus_tag="ACG90W_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0477488.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1650..1871
                     /locus_tag="ACG90W_001111"
     CDS             1650..1871
                     /locus_tag="ACG90W_001111"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0477489.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(2028..2192)
                     /locus_tag="ACG90W_001112"
     CDS             complement(2028..2192)
                     /locus_tag="ACG90W_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0477490.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2294..2815
                     /locus_tag="ACG90W_001113"
     CDS             2294..2815
                     /locus_tag="ACG90W_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0477491.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2802..3242
                     /locus_tag="ACG90W_001114"
                     /pseudo
     CDS             2802..3242
                     /locus_tag="ACG90W_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3282..3440)
                     /locus_tag="ACG90W_001115"
     CDS             complement(3282..3440)
                     /locus_tag="ACG90W_001115"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0477492.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3533..4294
                     /locus_tag="ACG90W_001116"
     CDS             3533..4294
                     /locus_tag="ACG90W_001116"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0477493.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4481..5833
                     /locus_tag="ACG90W_001117"
                     /pseudo
     CDS             4481..5833
                     /locus_tag="ACG90W_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5849..7069
                     /locus_tag="ACG90W_001118"
     CDS             5849..7069
                     /locus_tag="ACG90W_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0477494.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            7104..7781
                     /locus_tag="ACG90W_001119"
     CDS             7104..7781
                     /locus_tag="ACG90W_001119"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0477495.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7778..8971
                     /locus_tag="ACG90W_001120"
     CDS             7778..8971
                     /locus_tag="ACG90W_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0477496.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8986..9312
                     /locus_tag="ACG90W_001121"
     CDS             8986..9312
                     /locus_tag="ACG90W_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0477497.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9316..9651
                     /locus_tag="ACG90W_001122"
     CDS             9316..9651
                     /locus_tag="ACG90W_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0477498.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9648..10043
                     /locus_tag="ACG90W_001123"
     CDS             9648..10043
                     /locus_tag="ACG90W_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0477499.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10040..10411
                     /locus_tag="ACG90W_001124"
     CDS             10040..10411
                     /locus_tag="ACG90W_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477500.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10427..10987
                     /locus_tag="ACG90W_001125"
     CDS             10427..10987
                     /locus_tag="ACG90W_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0477501.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            11112..11423
                     /locus_tag="ACG90W_001126"
     CDS             11112..11423
                     /locus_tag="ACG90W_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477502.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11447..11638
                     /locus_tag="ACG90W_001127"
     CDS             11447..11638
                     /locus_tag="ACG90W_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477503.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11638..15486
                     /locus_tag="ACG90W_001128"
     CDS             11638..15486
                     /locus_tag="ACG90W_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0477504.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15487..16359
                     /locus_tag="ACG90W_001129"
     CDS             15487..16359
                     /locus_tag="ACG90W_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0477505.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16356..17405
                     /locus_tag="ACG90W_001130"
     CDS             16356..17405
                     /locus_tag="ACG90W_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477506.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17409..18233
                     /locus_tag="ACG90W_001131"
     CDS             17409..18233
                     /locus_tag="ACG90W_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477507.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18251..20311
                     /locus_tag="ACG90W_001132"
     CDS             18251..20311
                     /locus_tag="ACG90W_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0477508.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20328..20477
                     /locus_tag="ACG90W_001133"
     CDS             20328..20477
                     /locus_tag="ACG90W_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477509.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20474..20920
                     /locus_tag="ACG90W_001134"
     CDS             20474..20920
                     /locus_tag="ACG90W_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477510.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20922..21059
                     /locus_tag="ACG90W_001135"
     CDS             20922..21059
                     /locus_tag="ACG90W_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0477511.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            21094..21336
                     /locus_tag="ACG90W_001136"
     CDS             21094..21336
                     /locus_tag="ACG90W_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0477512.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21352..21549
                     /locus_tag="ACG90W_001137"
     CDS             21352..21549
                     /locus_tag="ACG90W_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0477513.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21560..22576
                     /locus_tag="ACG90W_001138"
     CDS             21560..22576
                     /locus_tag="ACG90W_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0477514.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22691..23899
                     /locus_tag="ACG90W_001139"
     CDS             22691..23899
                     /locus_tag="ACG90W_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477515.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            23883..24053
                     /locus_tag="ACG90W_001140"
     CDS             23883..24053
                     /locus_tag="ACG90W_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477516.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT         8406 a         4056 c         5278 g         6382 t
ORIGIN      
        1 ctccttctaa attgttctta ttatctatca tccgttgctg tctattgttt ttttaattat
       61 tcacacgata actaaaggtg agcgaagaga aatgattcca ttaataattt caatttttgc
      121 gctttgtctt aatgtctata tgattggatt taaaaatggg caaaataaaa gatagtagta
      181 actaagaaag aaatttttat attgtcactg tggcggaaag ggtagacgct aagcatgtgt
      241 gctaggtcaa tgcttcggca actatgcaat gttcgattca ttgccagtga ctttggcaac
      301 cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg
      361 tgtaggttgc tattacatat tagatcactc gttgagtggt ctttttattt tacataaagg
      421 aggtaacaac gatgtataga ccacaatact tagaacagaa gtatgaagta atcactgtgc
      481 aaaacggtaa cggtgagata gtacgaaagt atagaagacc aataaagagc gatacatata
      541 aacgaaagga aaacaatgaa gttatttctt ttcgcagaag gagaaaagcc aaatgagaaa
      601 ctactggtat gtatcgctaa ctaatgaata tcctcgaacc attgatgatt gttcagtgcg
      661 tgttgtgcgt tctgtacaaa tcaaagggaa gtactctatt gtcgaaatgc taagagaagc
      721 tacaccaaac gaagtggata aatgcaagct gatatattgc ggtcatggct attggaaaga
      781 cgagtatatc caacaaaaca ttgagaggtg gatagataga tgagttacct cgaacatttg
      841 aaacgttgct acatgcattc taagaataaa cttcctgaca gctacactgt agatgatgtt
      901 gctatacatg tattgaagac tgagagccat agcagtcctg atggtagcag taaggagcag
      961 acgctcgcgt ggttcaagtt ctttaagtgg attaaggagg aagagtaatg cacatgcaca
     1021 taggagaaaa gatatataaa ttgagacggc ttaataacga aacaatgaac gacttagtta
     1081 aacaaactgg agtgtcaaaa tcatggttat ctgatattga gtctggaaaa aagcaacgcg
     1141 ttgattttaa taagatatgt gcaatagcca aacattataa agttagctta gaatactttc
     1201 gggatgatta tttggatagt gatgaacacg aggatattat tcctttgaca ggcgaaacaa
     1261 gagttaaatc agtgaaatcg cttgaacaaa gcctaaagta tctgaataac aaacaagaac
     1321 ctaaccttct agagatcaaa ctaaaagata ctgactcagt accagaggtt tggtacaagg
     1381 gtgagaggtt ggatgaattg cctaaaggat tagtagatgt ctcgtatcat tggaagactg
     1441 atgattttac taatgatgat agaggagcga acgacatcac gattcaatac ttttctagct
     1501 ttaatgataa gtatccagat aagaaaacaa tcggacacaa gagagatatg taaatgaaag
     1561 aagttagacc tagagacgaa atagacaaac tatacaagac caaacgatgg cgagacctaa
     1621 ggcaagtagt aatagctagg gacttcggca tgtgccaaga gtgcaagcgt cgagggcgga
     1681 acacaagggg cacgatcatc catcacatag tcgaggcgag ggaagacctg tcactgttct
     1741 ggtccgtaga taaccttgaa tgtatctgtg tagcttgtca caacagagag catccagaga
     1801 gatcaggcgg gaagaagaaa ccaaaaccta aatcacatat cgttaaaatg tattcaactc
     1861 ctgaaagata agtttgcagc gaaatgaagg tagcccccct actctaaaag attaaagagt
     1921 aaggcttgag aagaacggtg ctgtccttcc ttcgtaaaaa gaccgctttt caagtttttt
     1981 ggagaaaaag gaaaaagccg accaatttaa gccggctttg gacgaagcta tttcttagtc
     2041 catttgtttc ctttttgaga agtagggggt aatcggtcgc ctggatcaat ttttacttct
     2101 tgtccgcctt ggacatttcc accacgagga cccacttctt tgtaggttcc ttttggtttg
     2161 ttgtcttcgc cgggtttata gagttctccc ataggaatcc ctccttaaca aatttcggca
     2221 cagcactgcc gataacctaa ttataaggat tgtgataacg atttcaatct atcttttgaa
     2281 agaaggtgat attatgccgc aaccagcgaa gagtgcaaaa ttacaattat taaacggaaa
     2341 cccaaataag aagaataccg aagaactccg caagcgagcg gccgcagaag acaaattaaa
     2401 aatggctact gacaaaatca aaccgccgtt atggctagat tcgctaggaa aggatacctt
     2461 tgagtttatc gccgatgaat tgctgtctgt ggatttaatc agtaatccgg acgtccatac
     2521 aatggctcta tactccaatt ggtattcgca atacgtttct ttagaaaaac agcttcgaaa
     2581 actacaacga gagtacaagt tgaactatgc gcttgcgaaa aaggaggcag aggcgagagg
     2641 tgagccgttt aatgaaccta atgaattaat tggtaatccg ctctctcggc agatggatac
     2701 agcgtctcgg aatctccgtt cttttggcgc tgatttagga ctatcaccag cagccagagc
     2761 taagttagct attaagatgg ctgatgatgg tggtgatgac gatgacgact tctaatattt
     2821 tggatatgtc ttacacagaa cgtgtggact attggcaaag ctatcttgag gagcaagctt
     2881 cttggggtgg ctttttaaaa tgtccatatc cggaattgtt aactacttgg tatgcggaac
     2941 gattaatcga tggaagcata ccagccagca aagaaaatat tcaagctgct aaacggcata
     3001 tgcgtgattt gcagcgccaa ggaacagatg attttccttg gatctttgac gaagaaaaag
     3061 gtcaccggcc tattagatat atcgaaaaaa aatgtaaacc aactgaaggc gactttggtt
     3121 cgtttgtttt gcaaccttgg cagcatttca taattggatc catgtacgga tgggtacatc
     3181 gtgatacagg agagcgtcgc ttccgcgagg ctcttatttt tgttggacgt aaaaacggta
     3241 agttttgccg tttaaaaatc gggcaaaatc ggtgaaaacc tttatttttg gctttctttt
     3301 agtaataatt cgatagcttt atcaagcaat ttagacatag gaaccatagt ttcttccgac
     3361 atttttttta atccttcgta taaatcttta tcaattgcgt tagaaattct aattctgttt
     3421 tttagtccgt atttgtccat gtgtgacact cctttttatt ttactataac atccgattcc
     3481 accgcttgca actaccatca attgatggta gtatataaaa gaggtgagta gattgaaaaa
     3541 atacattatt tacaaacact taaataaaat aaatggcaaa atttatatcg gtgtcacaaa
     3601 tgatattggc agaaggtgga ggagcggtgg gattgaatat aaaccaccaa agaacgaaac
     3661 ccaacaccac agaagttttt ggaatgcaat acaaaaatac gggtgggaaa attttgatca
     3721 tttgattatt gaagaagact taacgatgaa agaagctttt gagaaagaaa agttttatat
     3781 tgaactttat gactcaacta ataagaaaaa aggttataac atcgcaaaag gcggaaatgg
     3841 cggaataata tataaagttc accccaaagg aatgctaggg aaaaaacaat ctaaagaatt
     3901 ttcttcaaat cacagtaaat gggcaaagaa ccataagaat aattgtatga caaacggtga
     3961 tgttgtatgg ggtgttactc acgaacaccc aaaaggcatg ttgggaaagc accacagcaa
     4021 agaatcaatt atgaaaaaga aagcatatag tggtgaaaat gctgtaacct caaaaccaat
     4081 tgtcgcaatt gaacgggatg gaactaaaag agaatttcac agcgctaagc tttgcatggc
     4141 ttactattcg attagtacat gtgtttttta ccgactttta aaagatggcg ccccttatgt
     4201 gataaatcct aaagcgaact acagaaataa agaaaaaata ttagctatcg aaggaattat
     4261 gttcaaacat aaagaagata ccgaggtaag ttagtagatt gcgaaaggtt actaactacc
     4321 gtaacgcgta ggagttgaat aaatataatg ctcccaagag tgtccgacag cagataattt
     4381 gctgaaaatg tacgctaaac tgggccagaa ctgactgacc gatgaaaatg agggtgacct
     4441 ccagagtgcg agataaaaag ctcgcagata ataacaatcg aaaacatcac taatttcagg
     4501 cctttccaca tacatggtcg cttatgatga tgaacaaggc gccaacgttt acgtattggc
     4561 aaatgctcgt gatcaagcaa gcttgttgtt tgataaggcc gcagaaatgg tcaaacaatc
     4621 gccggcgctc tttaagaaat ttggtaagcc taaacgatca agtattaatt atgctcccgc
     4681 cttttctaaa atggaaccac gcgcctcaga tagccggaaa ttggatgggc taaacactca
     4741 ctttggtatt tttgacgaga tccacgagtt tacgaattac aatctgatca acgttatcaa
     4801 gaaatcaaga ggaaccagaa aacagcctct gatagtttat atcacaactg ctggatatgt
     4861 attagatggt ccgttgatgt cttattttga gcaaggtgtg gactgtttgg aacatttgga
     4921 agatgacatc gatgaacgga ctttctatta tctggcaaaa cttgacagtg cggaagaggc
     4981 tgatgaccca agattatgga tcaaagccaa tccgaatatt tgtctaatga attttgttgg
     5041 catgctagat gactatgtta aggataaaaa agatccaaaa gaatatgctg actggattac
     5101 caagcaattt aacttgtttt ccgatatcga tgagctgtca tttgtcgata tgcctaccat
     5161 taaacgaaac aataaaacca tcgatattga aacgctcaaa ggtaagaagt gtgtcggtgg
     5221 ttttgacttg tccgaaacgg aagactttac cgcagccgtt ttagaatttc cgcttgaaac
     5281 aggcgaggta ttcattttgc aacacacatg gatcccacaa gctagatttg atcgagataa
     5341 caatcaagag cgtatcaaag cgtgggagaa ggtgggagat ctaacgatta ttcctggtga
     5401 ttacgtcaat tatgaatacg tcttaaattg gtttgtagaa aattcgaaaa tctatgacat
     5461 tgtaaaaatc aattatgaca aggccaaggc gctacgatta aataaagaac tagaaaatgc
     5521 aggatttgaa accaacgaga ttcggcaagg gtttctatca ttaggtgggc caatgcaaaa
     5581 cttcaaggaa atgctattgg acggtaaggt gattttcaac aattccaagc tttaccgatg
     5641 gtatctatcc aacgtcaagc tggtgatgga tcgcaactca aactggatgc cgtctaagca
     5701 gtccaagagt agaaaaatag atggttttgc agcaagtttg aacagccacg ccgaagtgtt
     5761 gaatatgttg gttaatcctg tcggaaccgg gaaagtaacc tattactcga tttccgattt
     5821 aatgaatatg taagaaaggt gtggaggaat gagtatttta gatcgtttgc gttcttttgg
     5881 ccgagcgaag ccgaaagcga gcaaacaaga gtattttttg aatgacccgg gattgatacc
     5941 gtatttagtc ggaaaagatg aaatatcaga agggattttt tccgtaatta gccgtgtatc
     6001 gaacgttttt gcgtctctcc ctctcaaaat gatagatgtg gagtttggcc aaccggacga
     6061 ctgtcctgca tacaacttgt tgagcgaagg ccctcgatat tttacaaagt ttgatttttt
     6121 ccgggacgtg gaagttttga gaaactacca agggaatgcg tatgtgcaga ttttccgaaa
     6181 tatcaatgga gaagtagcag atatggcgtt agtaaaacct ggtgcttgcc atccagtgat
     6241 tgatatggat agcggggagc tttactacca agtaactgcg actgacaaag gcagttacaa
     6301 gcaagttatc tatgtacatt acatggaaat gcttcacttt aaacaaccga ggtttggcgg
     6361 cttggaaggt acagacccca caaaagtatt aacgaatacc ctcggatatg atcgagaagt
     6421 ccgaaaaatc tctttaagtc agcttaaagg aagtaatgaa gggctaaaag ttaagtttgc
     6481 tagcaatatg gatgaagaag ctaaaaaagc tacagttaaa aacattgctg atttttatcg
     6541 acaaaacggt ggactacttg tggaagaaaa cggtgtagaa atcgaacgtt tacaacgaga
     6601 gctggtagac agcaagcttt tagatactga taaagtatct cgctccagaa tcgctatggt
     6661 ctacaatgtg ccggagcatt tcatcgggaa taaccagtcg agttactcct ctcaggaaca
     6721 gctcaatatg gaatttttga cgtacaatct agtaccgacc gttaatcaat atgaagcgga
     6781 actaaataag aaaacactat cgagaaccga aaaagcaaaa ggttatcgat ataagtttaa
     6841 tgtcgcaagt ttgctaagag ctgacactca ggcaagggga caattctatc aaatcatgcg
     6901 acgaggcgga gcatactcag cgaatgatgt ccgaagattt gaggacttgc aaccgataaa
     6961 taaaaccggt atggatgatt accatatttc cggagaccta tatccaatcg atatggatcc
     7021 aacattaaga aaaacaacct cgtctaaaag cgtagccgaa aacggttagg tttttttagt
     7081 ttgcaccgaa gggaggtgga aaaatgaaaa aagtgacgtt aagcggcgat gtcgtggata
     7141 acgataccgc gtggctttat gactggtttg ggatcaattg tatctcacca gggaaaattt
     7201 ctgccgctct tacagaagca gcgggggatg aagtagaact tgatatctca tcgaacggtg
     7261 gggatgtcct agcggcaagc gaaatatata ccgctatccg tgcctatccg ggaaaggtat
     7321 ccggaaatgt tgtgagtatt gcagcaagcg ctgcaagtgt aatcgcttgc gcttgcgaac
     7381 cgcttagaat ctcacctacg gcacacatca tgattcataa cgcatgggtg accactagtg
     7441 gcaacgctga ggaattaaaa gccaatgcag aaatgttaag cagtgtggat gagtctattg
     7501 ttaatgctta cgagatcaaa acaggactcg atcggaaaaa acttgctgat ttaatggcga
     7561 aagatacttg gttaaatgct caaacagcag tagcagaagg gtttgcagat gaaattatgt
     7621 ttgcagaagc accagtaacg gtactcaatg cctctcaacc ggttattcca aaaaacgcag
     7681 taactaagtt gaaaaattta atactcaaag cggaagcacc gcaagaagaa acactcttac
     7741 agaaaaaact aaaagcctta aatggaggga aaaacgaatg aatttagaac aattaaaaaa
     7801 tgcgtgggtc gaggcgggaa gtaaagtctc tgacttaaat gcacaactca atgcagcatt
     7861 ggttgacgat gaaaaaacag aagaagatgt agtaagtttg caagcacaag taaaagcagc
     7921 acgggctaaa cgggacggat tgaaagagca agtggcaaat atggaagccg aacaagtctt
     7981 aaacgtcaaa aaagaaccat tagataaaaa agatgaaaac ttgaaaaaca agtttatcaa
     8041 agactttaaa gcgatggtca atggtgatcc tgctattatg gctactttga catctaatac
     8101 ggatgaatct ggtaatgcta tcggattgac tattcctgta gatgtgcaaa cgactattca
     8161 tactttggtt cgtcggtttg actctttgca agaatacgta aacgttgaaa aagtgaccac
     8221 taccagcggt tctcgcgttt atgaaaaatg gtctgatatt acaccgttga ctgctttgga
     8281 tactgaagac ggtgaaatcc cagcaaatga tgatcctgca ctttacttga tcaaatactt
     8341 gatcaaacgc tatgcaggta tttccacagt aactaacagc ttgctaaaag atactgccga
     8401 aaacattttg gcatggttgt ctaaatggat cgcgaaaaaa gtagttgtta ctcgcaatac
     8461 aaaaatcttg gcagctattg atggaatcaa agcggcacaa aagaaagatg ttacagatgt
     8521 tgatggaatt aaagatatcg taaacgtcca acttgatcca gctatcgaag ctacatctat
     8581 gtttattaca aaccaagatg gcttcaatgt tttagataaa gtgaaacgtg ctgatggatc
     8641 ttacttgtta caaaaagacg tagcttctgc aactggatat accttcttag gtaaaccaat
     8701 caagaaaatt gcttctcgtt tcttgccaaa caaaggtacg caagctactc ctaaatatcc
     8761 actgtacatt ggtgatctga aagaagccgt tacattgtat gatcgcgaaa acatgagctt
     8821 gctgacaacg aatattggtg gtggagcttt tgaaacagac accactaaag tacgcgtcat
     8881 cgatcgcttc gatgtgcaac tagttgatga tgaagcggtt gttttggcta cttttacaac
     8941 tattgcgaac gagacaccgg cgaaagttta aggagctgat tccttatgat tcttgatcct
     9001 aaaatggatt taggcgaaat caaaaacgca ctaaagattg ataccgatga tgacgatgtg
     9061 gaagtaagcc gtgcggcaca agctgcaatt gcatacatta gaggggctat cgggaatgat
     9121 aagccctctt tttataagca agaaaatgaa acggttgatc tgattaattt agctatcctg
     9181 caattagcag atcattatta caacgctcga tcagcaaccg tgagtgggaa cttgcgagag
     9241 tacgatttag gttttacaag cctaatcttg caactcaaag caagttattt gctttttgtg
     9301 gaggaggagt agcgtatgcc ccttatccaa acaggaaatt taaatcaacg catcaagttt
     9361 gtccgagata cgacggttaa ggatgaggac gggcaagttg tcccgacttc tacaaccatt
     9421 cttacttgct gggcaagtgt gcagacacaa cgcctgaacg atattaagac gtcgattggt
     9481 acggctttgg aaggaacact gacgttcatt atccgctacc aacaaaaatc agagctgacc
     9541 aatgatatga aagtgcgttg gaatggaaaa acgtttgaaa tcattacgat tacgaaaggc
     9601 gagtttgcga aggacttcac gacaatcatt gcaaaagagg tttcaaaatg agtgtagaag
     9661 tcgatgcaac cgaagtgtac aaagcgctta gggaagtaaa agcaaacgtt caacgagtgg
     9721 aaagcccagc acttagaaaa gctggggagt acgctcaaga agagttacga caaaacacac
     9781 cttactggga tggaacgaag tcaaacggta aacgtggttc gtatatgcaa gaacatgcta
     9841 agaaccatgt ggttacaagc tcgataaaaa acggattggt agaagtcggc tatgacaaag
     9901 atgtttcttg gcggatgcac tttatcgagt tcggaacaat caaacaacgt ccaaaaggtt
     9961 tcgtacaaaa aacacaaaag caaatcgaaa aacaagtaac acaaatcatt gctgacgaag
    10021 taaaaaggag gctaggactt tgaaaacggc agtatcacaa gtctattcaa ttctgaatag
    10081 caatgaaaaa acaaagaaca ttgattttta caccaatagt gttccggaat cagctcaaac
    10141 agtacctagc cttccagttg gcagaattac agagatatcc ggcaactatg aagattttgc
    10201 aagcaacaat cctttgacca ttcaatttaa cgtacaggta gatgtatggg tgtcaaccat
    10261 gaaagaggtt gatgcctttt attttgccct tgatgaggtt atgaggggga atggttggca
    10321 atgcgcatac acggaacaaa cagatgacga ggacttggaa ggtgcaaagc ggattatcaa
    10381 acgatatgta gcaaatattt cactaaatta aaaggagaga aaatagatgg caacagtagg
    10441 attcgagagc gtcatttttg gcgtaaaaac aggtgcaggc ggcactctaa aagaattagt
    10501 agcagataag tcgaaaggcg gagcgatcga agctaaaatt actggattag gcgcaacttc
    10561 taacacaaca tacgcttcaa acgtaccgtt cttcattgca agtaaagggg tttcgtcgcc
    10621 aaaagttacg cttgacgtgg cagacttaat ggataacggc atttacagcg aaatcattgg
    10681 tgctaaaacc gtggatggtg caaatgtaat tggttcagaa actgaagcgc cttacgtgtc
    10741 ggtagtcatg gttacagcga acaaagaagg aaaacgcttg ttcatgggat tgacaaaagg
    10801 aaaattcagt catccagata tcgacatgaa aacagctgaa gacaaagggg tagaattgca
    10861 aaccgattcc atcgaagggg aattcatttc tgatgaacgt ggctatgtat acttaacagc
    10921 cgtagaatca gaaggaatga ccttacaaaa attcaaggac ttggtaaata acaaagaggg
    10981 ggagtagtta accctgcatc tacaccaacg acagatacag ggacaccaaa agaaccagaa
    11041 ccaaaaattg atacacaagg ttagccattt ttggctagcc ttattttttg taaaaacaag
    11101 gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa acattcaaac
    11161 aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag aaagcggaaa
    11221 aagcagacct aactgattta gaagcgattg attcaatgat tgcaattaca gcagatattt
    11281 tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg agcgagttag
    11341 taccggcatt agaaagtgtt tttgaacaaa taaatgaact gggaaacaat gaaaaaaagc
    11401 agacggcgag caaaaagaaa taagtttttc tgaagctagg aaagcaatgg atcaaatcta
    11461 caaagattta atcgaagcag gttggacgat gagagatgtg gacgaagccg actatcatta
    11521 tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg gtgctgattt
    11581 catcaaacaa tttttatcgg ctgaagactt agtaaaactt gaggaaggag gtaaataatg
    11641 gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga tagcactagt
    11701 tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc gaatatgcgt
    11761 gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag tcaaaagacg
    11821 aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt aacaaagcgc
    11881 cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa ccttaacaca
    11941 cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa cgacacaaaa
    12001 aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaatgaaat caaagaaaat
    12061 gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga atctggtgct
    12121 tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa agctatcgaa
    12181 gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc aaatgaaacc
    12241 gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc tagcaggcaa
    12301 ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaag aaatagaaga ttttggcgac
    12361 aagtccacaa ggtcagctag gaaactggac ggactaaaag acaaattaag ctcgctaaaa
    12421 agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag cagtgtagta
    12481 agtggcgtgc aaggtttggt tggcgaagca gtaaacgcat cggattcatt gatgaagttt
    12541 tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc gaaaaaagaa
    12601 atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa cacaaccgca
    12661 caattggcat ctaatgggat tcctaactat acagaactaa ccaaggcggc aggtaacttg
    12721 aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat gctaacgcag
    12781 acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga tgcgataccg
    12841 ggtgcttcag gattgttaca agacgctatg ttgaaaaacg gagcttatac aggaaacttc
    12901 cgtgatgcaa tggcgcaagg tcaaatcact tccgacgagt tcaaccaagc aattgtacag
    12961 ttaggtatga atgacggagc agttaaggca gccacttcta cagacacatt gagcggttct
    13021 tgggagcaga tgaaatccac tgtaataaat gggctacaaa gtattataga aaaaataggc
    13081 gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa ttctattcct
    13141 aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt tgaaaataga
    13201 gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt aaaagcattg
    13261 aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc ttttgtggcg
    13321 attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac aaaatctgaa
    13381 acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt tatgagcgtt
    13441 atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg gctttgggaa
    13501 aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa gaacagcgtt
    13561 gtgaatacgg ttcagaacct atggaatggc gtgaaaaact tcttcagcaa tggcgttgga
    13621 gatacgtgga ataaggtagt cggttgggta aaaaacattt tcaacaaagc aactgaattg
    13681 aagaacaacg tttctgatgt aatcggtaac ctgtggaatg gtatcaaaga cacattccgt
    13741 agaggtatcg atacggtatt caattggttt tcagaactac caacgaagat gaagaatgcc
    13801 attattggcg gtaaaaacgc cattgttgat gcgttcaaaa gtattttcaa cgcagcactt
    13861 aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt actagaaaaa
    13921 ttgggcgcag aacctctaca agaatgggat gtaccacaat acgctacagg tacaccagca
    13981 ggtgggcacc caatcaatgg tccaatgatg gttaatgatg gacgtggagc agaaacagtt
    14041 atcacaccag atggtagagc cttcattcct aaaggacgca atgttgttct aaacgcacca
    14101 aaaggaacgc atgtcttgac agcagaagag accgcgcaac ttcaaggttc taaagctcct
    14161 aagtatcgtt acaaaaaagg tactaacttc tttggtaaca tgtgggatag tgtgaaaaag
    14221 gttgctggta atgtaggtca cacaattaaa aacgtagtag gtgacgtgtg ggactttatt
    14281 tcagacccgg gagcgttagc tagaaaagta cttggggggt tagatgtatt aggtggattg
    14341 acaaagtatc cattagaagt aggtaaaggc atcctatcta aagcaacaag tgcactgact
    14401 gaaaaaatca ctgggttgtt ctcatctggt aacttagata cctccgtagg aacaaatggc
    14461 gtctataaat atttggctga tgttgctaag tctgtgatga agaaattccc aggatttgtg
    14521 gcaactagtg ggtatagacc aggtgaccct tattcacatg gtaaacgtaa tgccattgat
    14581 attgcattac caggcgtcac aggaggctca cctagataca cagaagcagc aaactacgct
    14641 tttgacaaat tcgcttccaa aattggttac gtaatcacta atgggaaagt tcgtgaccgt
    14701 tcaggacaat caggtcaacc agcaactggt gcatgggagc catggcccga tggagatcac
    14761 tatgatcatg tgcatttaaa cggtgtgaaa gacccacaaa acactcaaat ttcaggagat
    14821 agcgtgggag gcagtggggt agaaagatgg cgcaatgtag caattagagc gttgaaaatg
    14881 accggtcaat acagtactgc aaacttaaat gcattactaa atcaaatgcg tacagagtca
    14941 aatggtaatc ctaatgcagt taacaattgg gatattaacg ccaaaaatgg aacaccatca
    15001 aaagggttgc tccaagtgat tgacccaaca ttcagacagt atgcaatgcc aggattcaac
    15061 agcaatattt ttgacccact atctaacatc ttagcttcaa tcagatacgc actatcaaga
    15121 tatggctcac taacaaatgc ctatcgtgga gttggttacg caaacggtgg aattgtaaac
    15181 caacatcaaa ttgcggaaat cgcagaagga aacaagccag aaattattat tccgttagat
    15241 aaggctaaac gatcaagagc gatgcaatta ttgtctatcg caatggacaa actaggagtt
    15301 tctcctaaaa cttatggaaa tgcgacaact gtttccagtg attacgaaat cctagaagcc
    15361 attgaaaaac aggcggcaac aacgaatcag ctgttatcgt tattgctggc atttttcaaa
    15421 ggaaatagcc gaactgatag agatttagct ttagatattc agaagatctt ggttaggagg
    15481 atgtagatgc gaactgtatt actgagaaat agaataaacg aagaaattga tttgtccaca
    15541 gaagactatt ttgctactgg gttgagtaat atggggtttg aagtaaaaaa agaacatgtg
    15601 ggacagtggg ggaatttcag agaaagcagt gagagcgttg aaatatctga atttcagtca
    15661 tctgtaatca tttctgtgca tgggtttcga gaaaaagaac tgtacaattc acttgtgcag
    15721 ttcctatcgg aaggtccgtt tgaactggaa tttgcttttg acggcgaaac aatggtaaga
    15781 agatgcagtc taaaatcttt aagtaagact gaaatcgatc cgaaaacatc gttactaaca
    15841 gacactttag aactatattt cacatcaaac tggtattccg taaaacgcga aaaattaatt
    15901 cagcgaccga atgtagtgaa aacacgtggt aaggtttttc cgtataaaag atcttatatc
    15961 tacactcaaa acttgtggga gaaaaaaggt gtattcaaat tcaataataa ttctgtatac
    16021 ctaacgaata gcaaggaacg gatgtccccg ctaaaaattc gtgtgattgg gaagtgttca
    16081 aatccgtatt gggaagtaat ccaaaactca caaatcatcg caacggatgg atacttcata
    16141 gatatgactg aaacacagac tctagaagta tcaagccttt ttgaagatac aacggcgatt
    16201 ttaaaagata ttgcaggggt agaatcttct gtctaccaac aacaggatta tacaaaaact
    16261 aattttgttc aggccccaac tggagaattc agcattgtgt ttcatgttgg gggggcagac
    16321 gtagagattg agttatacga ggagcgtgat ctgttttgat tttagcagtc acgctttttc
    16381 atcgtgattt aatgctttat aacgaacatt tattctctaa cagttttgaa tttggtgtgg
    16441 acgagatcaa cgaagaggct agtagcttta cgatggataa gtacgttcct gtaaaaacag
    16501 gcgattttct actagcaaaa tatattccta gtggaaaatt tgcatatttt ggtgtaatta
    16561 cgtcgcagga agacgaaaaa atcagttgta aaagcttact tagtttagct gatagtgaga
    16621 taccgactgc acgtgtgtca ggagataact acgaagagca tatccggcgg ttgattgaat
    16681 actatttgct gaatgatcca acgaaacaac taaaagatat tttagacgtc aaagcagaaa
    16741 gtgcgacctc tcattcgtat caagctaccg atacgaataa acacaagtta agtgcgtata
    16801 ttctcaatgg tttcaaaaaa tacaacgtaa aatggtattt caagggaatt caaaacagaa
    16861 aaatttatac aggcatacgt gctgtaaatg aatcaattta cattaaagac aattcttctg
    16921 aatttagcga ttgggatgtg tttgttcagg cgccgggcgc tggaaacgag aacaagctat
    16981 taatcgttga taaagcaatg aaagatatag agaaaccgat aatactgtca acatggtatt
    17041 tggacgaaga aaacaatttg acacaagatg gatcgaaaga aaatatcacg aaaccaactg
    17101 tcaatttggt taatatctac gatcaaaccg cagaagataa agcatcctac gaagatgtgg
    17161 caaaatcaga gttgaaaggc aatacgtatt cgcatgaaat caaagtgaac gttgtaagaa
    17221 atgcaaaaaa tttgaatgtc gaaacgattg aaacagggat gtttgccacg atttcttata
    17281 aaggaaagat atacaagtcg gttttgacag cttggcgaat atcaagtgat aaggagtttg
    17341 tggaattgac tttcggaaac ataagaagtc gttttatgga ttattttgaa gataatgggg
    17401 gataaaaaat ggttagcaat gtggatggat atcaatttga aaacgtgaaa gtaagcgcag
    17461 aaaatgatgc tagactttat cacgttttat ataatcggaa aaatcaggtt attgatggtt
    17521 acgatcagtc tatgaattta tcttcaagcg gattaacagt aaaagttgct gcaggagcag
    17581 cgattattca aggtcgtatg gtcgttgttc gacaagaaga aagtataaca gttccagcaa
    17641 actcaagtgg ttatatagca ttaacagtgg atttgacaca agaagttata cctggatcta
    17701 ttcttccaga atcggaagaa tatgaatgga ctaataatca agtcaagcta gaatttataa
    17761 caaaagttat aaaaggtaat ttaaacaatg gtgataaggt ctataattta ccactatgct
    17821 cagttaattc tactggatca actgtttcaa tctcaaagat atcggatagt tacgagctga
    17881 ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat actcaaactg
    17941 tccaaccttc aaaaaaaatt tggcagacag tttcaggttt tttgttagta tggctcccat
    18001 acgaaaacgg gcaagcaatt gaggatagat atgtaactac gcctttttat aaggagcgtg
    18061 taacttttac taatgtttta ggagaaattg tttcaggatt tgatgactat cacaaaaagt
    18121 ggtttagtaa acgaataaac tataattcga atactaatat atttacaggt gcagcaagca
    18181 atgcaagtgg agataacgca aatatggtgc ttaggtatat agtttctttt tagttaggag
    18241 gtatggaaga gtggcaaatt tagaaattaa attatctgca aataagagac agccttatct
    18301 acgtcaccgt gttgttggta gagttggtga tggggggctc acaacaatca atgtacaact
    18361 tcttgaggaa gatgaaatta caccttttgt aattaatcta aacggtactt tgaaatttgt
    18421 gggcgaagtt tcaaacggta actataccga gggagaacca gaaataatcg attcgactaa
    18481 tgggttaatt agttacacgt tcactaagtc aaatttcagc acgagtcatc aattcaaaca
    18541 agcatatttt gaatatgtag atcctaacgg caaaaaagta acttttcaga acttcatcat
    18601 agacgtgtta gaacgagtgg atattaattc ggagcaagcg aaatactata tttcttcatt
    18661 ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg ataaacaggt
    18721 ccaatacgat caaatctact cgaaatataa cgaattagta agattgataa atgaatcaga
    18781 taagcaagta aatgatcgta ttgatcaaac caatcagcaa atcggcgatc tcggcaagct
    18841 gaaaaagatg tacagtaaca gcatcgactt tgggggctat gattattcgg ggaatccgaa
    18901 tttgttaagt aagctatcat acgacttaat tgaaaatcaa aatacttcag ctggaacact
    18961 ttctaagggt gaaaactcgt ttaaatataa taagatatca gctgaagtgg aaggtggagt
    19021 agagttatat tataaacgaa gaggtatagc taactggtta ccctctaata aaacgcttgt
    19081 aatgactgtt aagcttagag ctggagcgga ctatagtcca gttgacggaa aacttatact
    19141 gattagatat aggtatgttg acagtggaac tggcaagatt gttttagact tacctattaa
    19201 cagtaattcg ataactcagg aatggaaaga gtttagtatt actggaacta ctccaacatt
    19261 tagcccacaa gcataccatc cttggataca atttagggct caagatgggg tacttgggga
    19321 aatagaaatg agctatgaca tcaaaatcga agaaggctct acagctacac catatcagcc
    19381 taacttactt gatgactctt actggctagg taaaacaccg ttgggtgaga atttgataac
    19441 gaatgccaag ttcccaatta taacacgaag caatcctatt tctggttttg atatttcaga
    19501 agaattaata ataggtgaaa cttatacggt atctttaaaa gggactaagc cagctactaa
    19561 agaattccac ttatactacg gagaagcaaa ttaccagcag ttgcagtatc aggcaacttt
    19621 acttcctgtg gaaggattaa cagatgtgtg gagtgccact tttacagcca gaaatactag
    19681 tgaaacgaca aatttactta gagtagcgtt atggcaaaaa cctaattctg caacatatga
    19741 tactgttcaa attgactggc tcaagattga aaaaggcgac acacgcacac caaatatcag
    19801 tcagtttaaa tactttggtg aaggcttgaa agacagtaac aatccgaacg actacagctg
    19861 ggatgtcaca ccagagtata ctgaaaaaag cttgaataat acggttagtc tgactgaacc
    19921 gcaatccatc gaaggattga aaaatttcga agatggtatt caatcaaaag gaaaatctgt
    19981 attgacatca gacgacaaca aatatgaagt cgtaacctta acagttacaa acgggaatac
    20041 cggatcagca aagctttatc gtgaaggaaa aaccgtcact atttattttt ttgcgttgaa
    20101 tgggaaaagc agtggtggaa atgattcaac gatactaaca attccagaag gctatcggcc
    20161 accaattagt tttgagcaac tggttggctc gatagaccgt tctactttga acagtgctca
    20221 gttatctatt ggtgcagatg gagccattaa atggcgaaga aactcaagtt atggatcgga
    20281 ttataccttt gcaattactt acacgattta gaaaggcgtg aatcgatatg aaggcagcat
    20341 atagaccaat tgaaccttac ggattcgagc aaatcattgt gaatgatgaa gaacatttac
    20401 cggaagaatg cacagaagtc gaaccaccga ttccaaattg gaaaccgaaa ttcaattact
    20461 gggagggaaa taaatgaaaa acatttggaa atatggccgt actggcggag agtatgcagg
    20521 aaaagtattg gacgacatgc ttgtatccgt tccttacaca gatcagccac cgctcgaagg
    20581 ggttcgttca gatggcgaac cgctaacgat tgctgatcag atgtttgatc ctaaattgaa
    20641 ccaatggatt attttagcga acgcgttaga tcacaacgat ttaaacaatc tcaaagcgat
    20701 gtacgaggct cttgaacatg aaaacggcaa cctaaaacag ctaaatgcca aactcatgct
    20761 aagcgatgta gcgattaaac aggaaaatac tgcattgaaa gaaaaagctg acagtttagc
    20821 acaaatcaat tcaaaaatga tgcttgcttc gttacaaaat agcaaagaca tttcagaaat
    20881 taaagagcaa ctaaatccag cttcaaaggg aggtgagtag tatgtttagt tttagcgatg
    20941 tgaaaatgat gtatgattgg ggctgtttta ctgacgatca agttcgtcta ttcgttccac
    21001 tatgtatcac agatgaagaa gcagatagaa ttattagcaa agaagagagc gcatcttaag
    21061 tgatgcgttt ttttgttgga aagttggtgg aacatgaaag aagaagcgct ccaagacgtt
    21121 gtggagagat tagtaagaat tgaaacaaaa ttagacaact acgaatcact tagagaaaaa
    21181 gctgatagtg caaaagattt ggcagacaaa gcctactcag tagcgctaaa caacgcagaa
    21241 gatatcaaag aaatgaaagc caataataaa tggtcgtggg gttacatgat cggtttaggc
    21301 attacgatca ttggctattt cttgactaaa ttgtaaagga gttaagaaga aatgatttta
    21361 cccgataagt attatcaagt cattaaatgg acggttttaa cagtattacc agctgcatct
    21421 gttttagtag ccacgttagg aaaagcgtat ggatggaatg gaacagatat gacagtactc
    21481 actatcaatg cagtagcaac atttttaggc gttatcactg gtgtgtctgc atataactta
    21541 aaaaaatagg aggaaaaaaa tgaagaagaa aatcatttta tcattgagcc taataatggc
    21601 tcttttttta ttgccaatta acgggttcgc ctatacgatc aacaatgaat ttaatttagg
    21661 tgtaaatgaa ggtagctcac aagtagcaaa taaccaatac atcctattgc atgaaacagc
    21721 aaacgaaact gcgacaggac gaaatgaagc gcagtacatg aaacgttctt ggtacaatgc
    21781 ctatacagcg tatattgttg gcgatggtgg gatcgtttac caagttggac aacctggtta
    21841 tgtacaatat ggcgctggtt catatgctaa tgcaaatagt cctgtgcaaa tcgaactgca
    21901 acatacgcac gataaagtaa cgtttgaaaa gaactacaag gcatatgttg aattagcgag
    21961 agattccgct aataaatacg gtattccact tacattagac acaccgtata atcaaccagg
    22021 aatcaaatca catttatggg taactcaaaa catttggggc gatcacactg atccgtatgg
    22081 atacttatca gaaatgggtg taagtaagga aaaactggct tacgatttag ctcatggatt
    22141 tacagacgaa aacccaacga cttctgagaa caagcctgtc attgatccaa cccgagcagg
    22201 tgcagcaaat ccaactttat cagatggaac gaaccattct cacattgatc agtttggaga
    22261 aatcgaaaat gcgaacttac acgtcgctgg atggcacatc gctaactata aatacgagta
    22321 tattttcatt atggactaca atactgggaa agaattagct cgagtaagag ctgatggaat
    22381 ttatagaccg gatgtaaatc aagcttataa tacttctgga aacgttggct atcatgtatc
    22441 ttttaacatg cgcaatttcc ctaataagaa agtatacgtc atgatgcggg caacgaatga
    22501 tccagaagga aacactaaag gcggtgcgca agatttccat gacaaacgtt ggtatttaaa
    22561 tattcctaaa cgataaaaat agctcctcgt tgaggagcag tacatagaat tgaaaattaa
    22621 cgttaaatca aaaaaatatt tacaagatgt ttatttaagt agataataaa agaaaaaagc
    22681 aggtgaaatt gtgacagcag aaattggtat tatgaataaa agtggaatcg tgttagcttc
    22741 agattcagca tctacgattg gagatagcaa agtatataat actgcaaaaa aattatttac
    22801 tttggattcc atgcattctg taggtatcat gatatatggt aatgctgaat ttaatggtat
    22861 tccttgggaa ataatcatta ctcaatataa gaaaagcatt ggtagttctg tttttaatac
    22921 tttggaagaa tacgcggata attttataga atttgtcaaa acagcatctt ttattagaag
    22981 tgaacagacc gagcaagaac aaatgattgg ggtttttcaa aaaataattt caggtttatt
    23041 tgagagtata gaagtagata ttaacttttt aataagccaa ggtacacaaa tagacaaaga
    23101 tgtcctagtg aagttattac aaacaaaaat gaataccaat ttgtctcaac agtcccagac
    23161 attcatttta gatattgaaa aggcgttatt tcttagtaac tatggagaaa tattaaaaga
    23221 tattcttaat agtatttcta caatggaagg tgtttcagaa gctatatcag aggaaattca
    23281 aagttacgtt tatgaaatta taattagaga cgatgtttat tcttccccta caggaatagt
    23341 tatagcagga tatggaagaa tggatatttt ccctaaacta tattcttata atatgtttgg
    23401 atttgttatg aatattctga aatactcaga atatgaaagt gctcaaatag gaaatgacaa
    23461 tggttcatta agatctacta tattaccatt tgctcagtct gatgtggtaa atactgtagt
    23521 tcaaggggta gatccacaaa ttactaatta tctgtcgagt caagtcgata gttttgacga
    23581 taacggaaaa aatacctata ttaatattat aaagaatatt tcagagtttc aacaaaatca
    23641 attcatattt ccgttactaa acatgattgc tttactccca gttgaagaaa cagcaattat
    23701 cgcagagact ttattaaatt taaccagttt taaacgaaaa tatactactt ctgtagaaac
    23761 agtaggtggc ccaattgatg ttttagctat tacacctaat gatggcccta tttggattaa
    23821 aagaaaacat tattttgata ttgataataa tattggatac agattgcgaa aggagagagc
    23881 gaatgattac aataattaaa cctcaggaaa aatcccctgt tttgaaattt aatgagagca
    23941 atctagccca taataaaatg agtataactg atacagtatt tgatgtaatg gttgagcaac
    24001 aaaaaaaaat taataataac aatagacaaa gaaaagaaat taaccaatat taatgttttt
    24061 tctgttgcgt tttttgcagt cataatttaa aacctcgctc aatcttgggc gaggtctttt
    24121 tt
//
LOCUS       DAZKOK010000032        23979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000032 DAZKOK010000000
VERSION     DAZKOK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.032"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..3339
                     /locus_tag="ACG90W_001141"
     CDS             61..3339
                     /locus_tag="ACG90W_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0477517.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3880..5787)
                     /locus_tag="ACG90W_001142"
     CDS             complement(3880..5787)
                     /locus_tag="ACG90W_001142"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0477518.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5917..6792
                     /locus_tag="ACG90W_001143"
     CDS             5917..6792
                     /locus_tag="ACG90W_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0477519.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6872..7846
                     /locus_tag="ACG90W_001144"
     CDS             6872..7846
                     /locus_tag="ACG90W_001144"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0477520.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8026..9171
                     /locus_tag="ACG90W_001145"
     CDS             8026..9171
                     /locus_tag="ACG90W_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0477521.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9308..9652
                     /locus_tag="ACG90W_001146"
     CDS             9308..9652
                     /locus_tag="ACG90W_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0477522.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9923..11209
                     /gene="efmA"
                     /locus_tag="ACG90W_001147"
     CDS             9923..11209
                     /gene="efmA"
                     /locus_tag="ACG90W_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0477523.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11347..11946)
                     /locus_tag="ACG90W_001148"
     CDS             complement(11347..11946)
                     /locus_tag="ACG90W_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0477524.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12350..13471
                     /gene="mnmA"
                     /locus_tag="ACG90W_001149"
     CDS             12350..13471
                     /gene="mnmA"
                     /locus_tag="ACG90W_001149"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0477525.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13717..15138
                     /gene="arcD"
                     /locus_tag="ACG90W_001150"
     CDS             13717..15138
                     /gene="arcD"
                     /locus_tag="ACG90W_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0477526.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15152..16270
                     /locus_tag="ACG90W_001151"
     CDS             15152..16270
                     /locus_tag="ACG90W_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0477527.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16330..17622)
                     /locus_tag="ACG90W_001152"
     CDS             complement(16330..17622)
                     /locus_tag="ACG90W_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0477528.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17772..18332
                     /locus_tag="ACG90W_001153"
     CDS             17772..18332
                     /locus_tag="ACG90W_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0477529.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18461..18838
                     /locus_tag="ACG90W_001154"
     CDS             18461..18838
                     /locus_tag="ACG90W_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0477530.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18771..20843
                     /locus_tag="ACG90W_001155"
     CDS             18771..20843
                     /locus_tag="ACG90W_001155"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0477531.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21054..22001)
                     /locus_tag="ACG90W_001156"
     CDS             complement(21054..22001)
                     /locus_tag="ACG90W_001156"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0477532.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22247..22711
                     /locus_tag="ACG90W_001157"
     CDS             22247..22711
                     /locus_tag="ACG90W_001157"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0477533.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22773..23816)
                     /locus_tag="ACG90W_001158"
     CDS             complement(22773..23816)
                     /locus_tag="ACG90W_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0477534.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7791 a         4401 c         5060 g         6727 t
ORIGIN      
        1 aaaataaatt aggtaatact ttaagtaaac gctttctaat ttgttacggg aggaatacac
       61 ttgttaaaaa aacgtataaa atacagtgcg ctaatgatga atctccttat gctcagtacc
      121 ccggttttaa tttctgtcgt tgaagttgct gctaacgaac aacagtctgt gaacgaagaa
      181 aatgcacacg tgaacattga agggatgtca acaatggagg aaataccaag aactagtgaa
      241 gatatggata cggaagaagg aaatgatcct atgatgaaag aatcagaaaa tgaaaagaca
      301 aaaaagcaat ctgaagaagc aaatgaagag tggaaagggg ttgttttcgg agaaagcaca
      361 agtgctagta ggtatgccat tgaaccatta gaagacggcg tgcggctctc ttccacgaat
      421 aacggcggaa aattccagtc aagcggatca gatggaatga cctattatta tcaagcaatc
      481 cccaaagaca tcaactttag gatgcgggcg acgatcgaaa tagaatcttg gacctatacg
      541 aatgcccaag aaggatttgc tttaatggta agagacgcag taccaaaaaa tcatttgttt
      601 gggcaggcat tttattcaaa ttcatttgcc atactaggca gtcggatcga atatgtctgg
      661 gactcggata gacaagaagt ggtcaacacg agcggcagca aatatacgat gcgacttgga
      721 ttggggacac gggcaatcac aggaatatca tctgaagatc cgcaagctgc gccagctcct
      781 gggagtgtct cagtggaaac aacaccttta gagacttcag cagggttctt ggaaaaagaa
      841 acaggttctt ataatatatt cggaaacggt catgggaatc tgtttccggc cacgaactcg
      901 attactaaac tggacgtaga gatgaaaaaa acgaatactg gtttagaggc atattattac
      961 gatcctgaaa caggagaaga aatggcttct gacatcatgt atgactggga aaaattatac
     1021 gcttcagatg aatcggttat ttatgcagga tttgctgcag caaggaatat gacgatcaag
     1081 gtagaaggaa tcgaagttgc ttactcagat cctgcgacag atcctcctgg gcaagaaaga
     1141 cctactcgct tgattgatcc gatttacact gtgacctcta gtaatcattc aggaaaccaa
     1201 gatcatacta tgtcctttag agcaaatgct gacggattat tgacaattaa aaataaagca
     1261 acaggagagg tgcttaagaa cgcaaaaaac ttggctatca ctgcgaatcg ggaatttgat
     1321 tatcagacaa aactgatgga aggaacgaac gaatttactt tcagttttac gccagataaa
     1381 aactatccgc cagaagaata cgtagagatg actagttatg aaacaaaaga gattcttcat
     1441 acagtggact accgtaaacc taaatattcg acagtttatg taacaccaga agggtcagat
     1501 gcaggaaacg gaagcagaca aaatccatta tctctgacac aagcattgag atacgcatat
     1561 gctgggcaga caatcgtcat ggcgcctgga acttattcct tcgaaagagg attgacgatt
     1621 ccaaaaggcg ttaacgggac aagagaaaac ccgattcagt tgattgcaga gaaaaatcca
     1681 gaaaacaagc gtcctgtact tgattttaaa ggaactggaa atgggatgac actgttcagt
     1741 cactactggg gtttacgagg gtttgacatt acaaatagtg cagctatgca aaaaggatta
     1801 cagatttctg gaaatcataa tttgatcgaa gagattcatg cttatcggaa tggaaataca
     1861 ggaatccaaa tcagcggtag ctcaaatgat cctttcgaag catggcccgc gcataactta
     1921 gtcaagaact gtacatcttt tgaaaatgcc gatccaggat ttgaagatgc cgatggattt
     1981 gcggccaaac tgacaatagg agaaggaaat gtatttgacg gttgtatcgc gtatcataat
     2041 gcagatgacg gttgggacct ttttgctaaa aatgaaacag gttcgatcgg aaaagtaatc
     2101 atcaaaaact cagttgctta tcgaaacggc tgggtaccag gaattgaggg agaaggtaac
     2161 ggtaatggat tcaaaatggg cggttcttct ttaacgggcc ctcatgaatt gatcaatagt
     2221 ttgtctttcg aaaatcttgc gaaaggaatc gattccaata gcggaacaga tattatcgtc
     2281 aatagcagta caagttttaa taacggttca tataatgtgg ctctttatac ctcttcagca
     2341 ggaaacacag actaccatgc ttctggtatc ttatctttcc gaacagagaa tcttgagatg
     2401 agagagcaga tccgcccatt gaatcaaaac gaagatcaaa tctaccactc gttgaactac
     2461 tattggaatg aacgagagaa acaatcagaa aatactcttg gacataccat atcaaaagat
     2521 tggatcgagt ctctaaatac atgttcgaaa gaaggtcagc agcctgttac gcgctatgaa
     2581 aatggaagta tccatgttca tggactgatg cagattaaac cggggaatcg tcaaacagaa
     2641 aaggaaagag tggatggtct tccattatct gtaggtgcta tttttgacgg agaaacctca
     2701 ccgtcttcgg aatatccaga gtatgcaatc gtacaagaac caaatcagac aaaaccaaat
     2761 gaacaaaata atccgattat tatacaagaa gcaaatggtg tggcggagaa tgcagaagga
     2821 aatatcattg aactgaagcc ttttacagtt tttgtgtcaa ctttcacacc aaatcaagga
     2881 acgcctgtct ttgaagatat gcgttatttc ccagaaaaga tccaaatcgt tgaaacacag
     2941 tcaagtgctc aattttctaa tcataccgct attttttcag caccttcaaa aggagaatat
     3001 acgctagaag tcacttatcg ttcggaaata tttgatgggg aaaaatggtc tgctcataca
     3061 gaatctgtta cccagcaaaa agcgatcact gtgcgagaaa ctgccgatgt gcccccggga
     3121 aatgacaaac cagaaagaga aaatccagga aatgaatcac caaatggaaa accaaatgga
     3181 aaaccttctc atccggatag gacagcaaaa gaaagtgaaa aaaatggcac tcaaggactc
     3241 ccaaaaacgg gagaatttaa taacccgttg ctagcattag ctggaggaat tttgttaatc
     3301 ggtgtagttg tttatgtcat gaaacagcga aaaaaataag tggaatttct ccttttatga
     3361 tgtaaaacaa ttgactgagg aaagtaaaaa tagaaggaaa tgagaccaat agatgtctca
     3421 cttccttcta tctttttata gatgaggttc tccttgctgc ttttgattgg ctgttcttat
     3481 tacttgatgc aggacagctt tttcacaatc tctccactaa acggtgttca atcagctgac
     3541 cctcggtatt agctcaacaa acggcaaaat atgaggaact atttttccgt ttttttctct
     3601 aatactcagg tcaggacgct gatttcacaa cctctcgata aacggtgttc aaaagccagt
     3661 ttctcggtag taagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc
     3721 ttactacttg aaactaagca gctttttcac aacctctaat acatggtatt ccagaagtaa
     3781 cttcttcgaa aaataagccg aaatgtcaca aaaatttgag aagcgatttt cccaaattcc
     3841 ttcttatttc ttgaagctga ccacttctgg cacaacctct taccagattc gcactctttt
     3901 ttctggcgct agatacatag cgtcttgttc cgtgatacca aatgtttcat agaattcgtc
     3961 aaggtttttt ggttgcacgt ttgctcggag tttagcaggg gcatggacat cgatttgcag
     4021 taaaagttgt tggtattctt tttttgcttt tgtacgccaa atcgttgccc aattgacaaa
     4081 gaattcctcg atagaaagat ctgattccgt ttttgctgct tccaatgcac agcttagacc
     4141 gcctgcatca gcaatatttt ccgaaacggt taattttccg tttacttttc cgtcagcaaa
     4201 aggcagacca tcgaattctt cgatcattgc ttgtgctttg ttttgaaatt gtgtaagatc
     4261 ttcttctgtc caccagttgt tcaagttgcc atattcgtca aataaagcgc cgttattatc
     4321 aaatgcatgg gaaatctcat gagcgataac agctccgatt ccaccataat tagcagagct
     4381 tgattgttct agactgtaaa atggtgcttg aaggatcgct gccggaaaga cgatcacatt
     4441 ccgaaatgga tgataataag cattgaccgt gtcagcactc atttcccatt ctgaacgatc
     4501 cactggtttg ttccatttgc tgaaacgatc ttttaatgtg agtttgctga aaacaagagt
     4561 atttgaaagc aaagtaccac cctgatctgc aggtactgtt ttgaactggt cgaataatgc
     4621 tgggatctta tcggggtagc ctacttggat acctaagtta tttaatttta tgacggcttt
     4681 ttcttttgtt gcatccccaa gccagtcatt ttcttctaga cgttttttat aaacggcgat
     4741 catttttttc accatttgtt ctacgtcttt tttagcagct tcgccaaaat atttttttcc
     4801 gtagtaatcg ccaactactt gatcgaattg tccagaggca aggtaatacg ctgctttttt
     4861 ctgaggcatt gcttcgtctg ttccagacaa tacgcgagaa tagattccgc tgacttggcg
     4921 gaattcttct gatagataac cactaagaga acgtactgtt ttaatgatca tccagctttt
     4981 gaataacgga aaatgagctt ctgtcaggat gtcattcaaa tgatcgaagt acactggatc
     5041 agttacgatc actttatctg gagttgtccc aatcaaaccc gtgacaagtt ttttcaagtc
     5101 cagtttatct gtgtatttaa taaatgtatc aaaagattgc ggattataca ttttgctgta
     5161 atctgcgttt tcttctgcac tttttacatg aggagcaatc aatttatcaa attgcactgc
     5221 ttgttccgtg attgcttctg cctgactttt ttctttgccg gcaagctcta ataactgaac
     5281 agccatatca aagaaaacgc ctattagttg tgcaccgttg ggatggtctg atgcataata
     5341 cgtcttatct ggaaggaata aagaaggcgg ataagcaaag aaggcgttcg tttgcgcatt
     5401 cttcatatct gcatctacat ccagattaaa tggcaatggt aatgtatcca acagccattc
     5461 tggaaattgc ttattcaatt cgtcgaaaga ctgcaatgat tcaacttttt cgattatagg
     5521 ttgaagcggt tgagcatgca actcatttct tcggtcataa tcgtttgcca gttgataata
     5581 tgcaaggaaa tgtttcattt tttctgaaga cactttttct ggatgggcaa gcagatcgtc
     5641 tgtttctccc atcaaaagtt ggtcgatccc gtcaactaaa tcttggaaac cgcctgtagc
     5701 tggtttatct gcagggattt ttgctgtttt ttgccattct tcattgactg cttcatagaa
     5761 atcttgtttc aatcgttctt tgttcattca tcgatcgctc cttttttgtt tcgtgtgccc
     5821 attatcttac aaaatgaagg aattaacaaa tagaacataa aaaattgaaa attcgaacaa
     5881 tcaaactgat ttgttttttt caatccttca cttataatga aaaaaaggag gcgatcccaa
     5941 ctgaatcaag tagtggataa acctttatat ttcaaagtag ataaaaaaat taaaaaatta
     6001 gccagcactc agcagctgca gtcaagaaaa agtaaattgc tttttttagc tctggttttt
     6061 gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc cggtagaata ttctccagca
     6121 aaatatactt atcaagaggg tctttcttgt aaaaaatgga agctgatcaa tgaagagcca
     6181 atcgagttat ctagatggat caacagaaaa gaagatattc ccgttttgat cgaagaaaaa
     6241 aggtcaggaa aggaactcgc aaattgctgg gtgggattgc cagaagaacg ttttttgagg
     6301 tataagaaat gggctacccc ctcaggctat ctttgtggta cttacgcggc agcagttctc
     6361 cttgcctatt atcaagacta tcgtaagggg tggatgcttc caaatgagat tcgcaagaaa
     6421 aatacggctg attcgttggt tttgacgaag gcattgcgta gccagatcca accactagga
     6481 cttcctacga ttccttttca ggttagtaca gggatttcta gttttttaaa gaagaatggc
     6541 aatcatgagc gtgcacgagc aactcttcta ggttcatggc agcgagctac aaaacgtata
     6601 cgtgaaggca aacctgtgat gattggtatt ttgaaagtac ttggcagtac atatggcaat
     6661 cattgggtga cagcgtacgc ttattttgaa actgaaacag gagaacgtta ttacaaagta
     6721 cacgacaact ggggcgatta tcataaagtc attcctgcca gttggagtaa cggaacagtt
     6781 tcacttcctt aagaatttgc tgaaattaac agaaaatctt ggaaaaatat gttaaactat
     6841 cgaagaatag agaagttttg gggaggacta catgagtaat aagtatcagg atgacacgtt
     6901 gaagattttt agtttgaatg gcaaccgtcc attagctgaa aaaatcgcga aagtgttcgg
     6961 aacagaatta ggtaaaagcg tcgtgaagca atttagtgat ggcgagattt ccatcaatat
     7021 cgaagaaagt attcgagggg atcatgtgta tatcgttcaa tcaacgaatg cacctgtcaa
     7081 tgattactat atggaactac tgatcatgat cgatgcaatg aagcgagcaa gtgcgaaaac
     7141 gatcaatgtc gtattgcctt actatgggta tgctaggcaa gaccgcacgg cgaaacctca
     7201 tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa gaagcagggg caactcgagt
     7261 actgacttta gatctgcaca cggttcaagt acaaggattc tttgatattc ctgtagataa
     7321 tttatttacg atgccgttgt ttgctcatta ttaccgtcaa ttaggtctga cgggagatga
     7381 catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt gcacgtagtc tttcagaata
     7441 tttgaacagc acattagcca tcgtagatca tgcagatgat gcttcaactg agggtggtta
     7501 tgtgatcggt gatgttcaag gaaagacatg tatcatggta gatgatatct tgaacacagg
     7561 agcaacgctt gcacgagcag caaatgtatt aaaagaaaac ggcgcaaaag aagtatatgc
     7621 ctgtgcgtca catggattat tgtccgatca tgcgaaaagt attttagaca atgcaccaat
     7681 caaagatatt tgtatcactg attctgtttt cacagaagaa aatcgtcatc cagaaaactt
     7741 aacaatcgtt acatgttcag aattaatggg tgaagcggtc aaacgcatcc atgaaaatac
     7801 accgatgagt ccgttattcc gtctggaaga aaaagaattc gaataaacaa aaaaaagagg
     7861 gaaagaagtt gtgacagaag tgagcagctt caaggagctg cgacaagact tttgtcacag
     7921 ctcctttttt ctatgcccat gaaacgtcct ctagacaaaa atgagctctt ctttggtttg
     7981 ttcgctattt tatgctagaa tgtgcctagc aataatttgg aagggatgaa tgaagtgaaa
     8041 actatttatc tggatcatgc ggcaactacc cccatgcatc cacaagtaat tgaagcgatg
     8101 acggcaatca tgcaagacac gtttggaaat ccttcaagca tacatggatt tggtcgccac
     8161 gcgcatgaaa aattggaaac agcaagacaa atcattgcag acagtctgca agcaaaacct
     8221 cacgagatca tctttaacag tgggggaaca gaaggggata atacagctat cttagaaaca
     8281 gcgttctcgc gtcaaaaaga aggcagacat ctaatcacta cagctatcga acatcctgct
     8341 gttcttaata ccatgaaata tttagagaca aaggggtttg acgttacata tctgcctgta
     8401 gatgaaaaag ggaatctgtc gatcgaagag gtcaaaaaag cgttacgaga agatacgatc
     8461 ttggtctctg tgatgtacgg gaacaatgaa atcggaaatt tgatgccaat caaagaaatc
     8521 ggtgaacttt tagcggatca tccagcttat ttccatacag atgcggtaca agcgtacgga
     8581 aaccaaacga tccatccaga agaattagga atcgatttat taagtatctc tgctcataaa
     8641 atcaatgggc caaaaggcgt cggtttctta tataaacgag acggagtaaa tatccctgca
     8701 ttgcttttag gcggggagca ggaagaaaag cggcgtgcag gtacagaaaa tttagcaggg
     8761 atctggggta tggcaaaagc agttgaactg ctgtcacctg aagaacgtga agcacgtaac
     8821 aacaaatata aaggattcca gcaattgatt cttgatacat tagaagcaaa cgaaatccct
     8881 tatcaagtaa atggtgattt aacgaataaa ctccctcatg tgttgaatct tcgcttaatt
     8941 ggcgaagcaa atgatttgct actgatgaag cttgatctta atggggtcgc tatttctact
     9001 ggttcagctt gtacagcagg aaatatcgag ccttcacacg tattagaagc aatgtatgga
     9061 gaagatacac cgatactaaa agaatccatc cgtatcagct ttggtttggg aaataccgaa
     9121 gacgacatcc agtatttcct tgatcaatta gttcgtgctg tcaaaaaata actagtgaag
     9181 tcattcttat atgaacagat gtctgagaga acgccttcat tttttaggga acttaactga
     9241 aaaacctagt aaaaatcagg ataaactttt ataataaaga gaaactaatt ttatcgaggt
     9301 gaaaatcatg gcatttgaaa caacagcaac ggtactgggc gcaccaatta gctatcgttt
     9361 gcatcctgat gcaaaaagat atacattgcg cgataatgga tttacagaaa caaacggagg
     9421 caattatcaa ttgatccgac cattagatgc cacgccgcaa agcaaagaag gattcaaatt
     9481 aaaaatcact gtatcaaaag atattcaaac attgaaaatg tcgatcacta cagcaaatgg
     9541 attgaaagca gtcaatattt tcaaagatcc aaaacagaaa atgagccaag ataaatttta
     9601 ttttctgatg gatgggatga ttagccgtgg gttattcgaa aaagtcgaat gatctgtgga
     9661 tataaaaaga caactagttt tgaaacggcc tattgttaga tgactaactt tgtaaggacg
     9721 acgcaaatta gttgtctttt ttatttttaa atttaacaca tgttgttatt tttaatattt
     9781 tgctgattta ttgtttctaa gtaaagaaat ttaaattgaa acgattgtta tttagggaaa
     9841 aaggtatttt ttgaaaaaag aatcccgtat aatgattgtt gtgcaaacgc tatacaaaat
     9901 gaaaaagaaa agagggatgt ttatggaaaa tgaacagtcg gtcgttttaa cgaattggaa
     9961 gagaaattat ttgttctttt tatcgggaca gtttttgtca ggaatcacta gtatggtcgt
    10021 ccaatatgcg atcatctggt atttgacaag agaaaccggt tcggcgacaa ttttgagttt
    10081 tgctacttta ctaggaatga ttcccatggt tttactaagc ccatttgtag gaccgctagt
    10141 ggatcgctgg gataaaaaag ctcttttgat tgtcacggat attattgtag cgattttcgc
    10201 gcttatttta gcagtagtcg gaacgatttc agaatccttc ccgatttggc tagtttttgt
    10261 gtctttgttt atgcgttcgg tcgcacaaac attccaaatg ccaacgatcc agtcgattat
    10321 gccgacaatc gttccatctt ctcacataac aaggacgaat ggacagttag gaatggtcca
    10381 atcagcgaat ttcatcattg cgccagctct aggtgcggct ttgttttcag tcgttcctgt
    10441 caattacctt attttattag atgtattggg ggcagttttt ggtgtcggct tattgatttt
    10501 tgtgaagatc ccaaaagttt ctcctgaaat attggaagtt ccgcttacta tttttaaaga
    10561 tgcaaaattt gggctgcagc aattgatgga taataaaggg ttatggtaca taacgatcaa
    10621 tggtgcattt gtgatgctgt tgttcatgcc agcaatcagt ctgtatccat tgatgacgct
    10681 agattacttt ggtggttccg ttggacaggc gggagcagtg gaagtagtct acgcagtcgg
    10741 aatgctctta ggcggtgcac tgatcagttt tataggaaca tggaaagacc gaatgaaacc
    10801 tattattata gcctacatca ttatgggcct gacgatcggt gcaagcggat tggttccaaa
    10861 cgatagtcaa gggttcttgt actttcttat cttgaatgca ggagcaggtt gtgcaacgcc
    10921 atatttcaat acactgctga tggcaatgat ccagcagagt tatgaatcga atgttttagg
    10981 tcgtgtctta gggaacttca actctttgat gaacctggca ggtccaattg gattattatt
    11041 tgcaggacca ttagctgatc gtttaggggt agaaaaaatg ttcttgtttt ccggaatcgg
    11101 gattttatta tgcggaatcg ttttattttt aacgtcagct gcccgaaatt atgataaaga
    11161 attacagaaa aaactggtaa aagaacacca tgagcaaaaa gacgaatagt atggaatgga
    11221 gttgtgacag aagtcttgtt acaactccat ttctgatcct aatgttcctg tggcaataag
    11281 cctactcaaa tgaaaaatat gagtaattat ctttatttgt ttaatacttg atatagggca
    11341 gttttttcac gacctatcgt acttcctctt tgcttcatag atttctttga tttgttgctc
    11401 agataacgga gtgccaaatg gtagtttttc ctttaagagt tctctttccg gtgcgtctac
    11461 gcctacattg acatcgttct cgataggtac gctgtaatga tggatatgtc catgaagatt
    11521 gcggatagtg tcgttaggcc cgagcaacat cggataatga gtcaagtaat attgttgatg
    11581 gtcgaattta acgatggcac ctacatcata aaaataaaat ttgggacttc cgtcagtaag
    11641 cctaggatca tttttttcta aataacggaa aaaagcgcga ctgtcatggt tccctttgat
    11701 caagtggatt gttccttgca gttgagtgac cagttccaaa atttctgcat ttgctttttc
    11761 atattgcgga tgcatagcaa ggtcgcctag atgataaaca tggtcggttt cttttattac
    11821 tgcattccag ctagtgatca actgttggtt catttcatcc acatttgcaa agagccgcgg
    11881 agcaaagtcg ttttttccta ataagcgttc atggaaaaag tgcatatcac tgataaagta
    11941 attcatgtat cttttgcctc ccttttttct tagagtaaac aaaatcactt atctgagcaa
    12001 ctcataacat ctaaaaagaa aaggttgtaa atgagacgtg cagctttaag cgataagaag
    12061 caattttgaa aaatagtttt tcacaattta taaaattatt ccttattgcc gaaaggctgg
    12121 ccctttttat gagtatgcca attttttata tcgaatgctc aaaagatgag tgattttttg
    12181 gttgactatc atgtttgtgt actgaatagg tccagtagaa cagtattttt acgacttgat
    12241 aattaaggga tgaaattttc agaaatatga cgtataatgt aaaatactga aaagttgcga
    12301 ccttcaactc gtagatgttt cagaatctaa ttgaaatgat ggtgatatca tgacagacaa
    12361 cagcaaaaca cgcgttgtcg tagggatgag cggcggtgtc gactcatctg taacagcact
    12421 tttgttaaag gaacaaggat acgacgtgat cggaatattc atgaaaaatt gggatgacac
    12481 ggacgaaaac ggtgtatgca cggccacgga agattataag gatgtagcga aagtagctgc
    12541 tcaaatcggg attccttatt actctgtgaa ttttgaaaaa gaatattggg atcgtgtatt
    12601 tgaatatttc ctagccgaat accgcgcagg acgtacacct aacccagacg tgatgtgcaa
    12661 caaagaaatc aaattcaaag cattcttgga ttatgcaatg gatcttggcg ccgattatgt
    12721 agctactgga cattatgcgc aagtcactcg cgatgaaaac ggcgtggtcc acatgcttcg
    12781 tggagtagac aataataaag atcaaactta cttccttagc caattatcac aagaacagct
    12841 ttcaaaaacg atgttccctc taggcggcat ggaaaaatca gaagtacggg caattgctga
    12901 acgcgcaggc cttgcgactg ctaagaaaaa agattcaacg ggaatctgct ttattggtga
    12961 aaagaacttc aaacaatttt tgagcaacta tctgccagca aaaaaaggaa atatggtcac
    13021 tcttgatggt gaagtcaaag gacaacatgc tggcttgatg tattatacga ttggtcaacg
    13081 tcaaggacta gggatcggtg gcggaggaga ttcgcaagaa ccctggtttg ttgtaggaaa
    13141 agatctagcg acaaacacat tatatgtggg gcaagggttc catcatcctg cattgtatgc
    13201 cacaagcttg gatgcaagtg aaatccactt tacgacgaat gaaccaatgc caaaagaatt
    13261 caagtgtaca gcgaaattcc gttatcgtca gcaagacgtt cctgtgacag ttcgcttatt
    13321 ggatgataat cgtgcagaag tcgtattcga cgaacctgta cgtgcaatca ctcctggaca
    13381 agcagttgta ttttatgatg gaatggaatg tctgggtggt ggtttgatcg accacgcata
    13441 tcaagaaaca aaagttttac aatacgttta agcgatatta aatgaatcaa aacagctatg
    13501 gctaacaaaa gaaacacctt ttcttttgga tagcatagct gtttttttgc aataaaatta
    13561 atttttattc attttatact tgaaatactt gtgtaaaaag ttcataatat ccaataatat
    13621 gaataaagtt gctttttgtt aagcaagagc agttgcaaaa tagaagggaa tcttttgcaa
    13681 gtgatttgtt caattttata agtgagggtg atggaaatgg aaaagaaatc gacgggtatt
    13741 agcaaatggg gactggttgc tctggttgtc agttcgtcaa tcggaagcgg tgtgtttggg
    13801 atcacaagtg atctggctag ttctgcagct ccagggccag cgatcctttc atggataatc
    13861 gttgggattg gtattttagc tttggtatta tcgctgaatc atttaggaga aaagcgtcca
    13921 gatctagatg gtggtatttt cggttacgcg gaagcttcat ttggtaaatt aggtggcttc
    13981 atcagtggtt ggggttactg gttgtctgcg tggctgggaa atgttgcttt tgcaacgatg
    14041 ctgatgagcg ccatgggaga atttttcccg atattcaaag gtggacaaaa tgttccttct
    14101 attttatttg caagtatttt tatttggggt ctgactttat tagtaaacaa tggagttgaa
    14161 ggagcgagcg tcatcaatac aatcgtgacg atctgtaaat tagtgccatt attcctattt
    14221 ttaatatttg tatttatcgc atttaaagtg aatatattca ctgctgattt ttggggtaac
    14281 gtatctgata atctagtgaa tggaacaggg cagcaaggaa cgatctggtt gcagattaaa
    14341 ggttgtttga tggttatgat gtgggtcttt gtagggatcg aaggcgcaag tgttttagcc
    14401 aaccgcgcga agaaacgttc agaagcccag caagcatcga tcattggtct gctttgttta
    14461 ttgtttatct atatccttgc ttcgatcttg ccatacggcg tcttatctca agaagagtta
    14521 gcaactattt cacaacctgc tatggcaaat attttaaaag gaatcgtggg aaattgggga
    14581 gcagcgctga tcaatatcgg cttgattatt tcgatcattg gttcttggtt gtcttggacg
    14641 atgctcccag cagaaacaac gatgttgatg gctcgagacg gtctgttgcc gaaaggttgg
    14701 ggaaaaacga ataaaaaaaa tgccccaact tattcgttag ttgttactgc aatcttgact
    14761 aatctattct tattgacttt cttagttact gattatgcct atcaatttgc ctattcttta
    14821 tgtacagcgg caattttgat ttgttatcta cttgtcggga tctatcaagt ccagttttct
    14881 ttaaaagaag gaatcaaaaa acaagtagtg atcggtggga ttgctgtagt ttttgaattg
    14941 atgggcatca ttttagcagg attttcttat gtccttttat gtagtattgc ctatcttcct
    15001 ggtttttgct tctactggat cgcttgtcgt gaaaccaaac atcaaattac gggaaaagaa
    15061 aaagtaatga ttgggctgat ttgtgtggcc gcactgcttt caatcgggtt gcttgctgta
    15121 ggctggatca agatataagg gggaaagaaa catgaaaatc gcaaatttcg gtgtagaaga
    15181 atggctaaat aaatgggaaa agaaagcggt atatgatatt gcacaaagct cgatcgaagc
    15241 attgacatta gacgaactcg ttggcttaga tgggactagc gtaagtaaat ttttcgaaaa
    15301 acgcaaaaac gaaccattgg actatggctg gatcgaagga tcagatgttt tcaaacaaga
    15361 agtcgcttct ctttatcaaa ccgtcgatcc ggaaaacatc ttacaaacga atggagcgac
    15421 tggtgctaat cttttagctc tatacgcgtt agtggaagca ggagaccatg ttgtttcgat
    15481 gcttcctact tatcagcaat tatatgacat ccccaaatct ttaggtgccg ctgttgactt
    15541 tgttcatttg aaggaagaag aagattggca gtttgattta gaacagctag aagccttagt
    15601 caaacctgaa acaaaaatca tctgtttgaa tagtgcaaac aatcctacag gcacattgct
    15661 agatcggaaa actttagaaa aaatcgcaga aattgctcgt acagtagatg catatgtatt
    15721 gatagatgaa gtatatgcgc ctttaacgga taaaggtgaa ttcttgtcga tcgtagatgt
    15781 ctatgaaaaa ggtatagcaa ccaattccct atccaaaacc tactccattc caggtattcg
    15841 tattggctgg acagctactg gacctgagtt ggcagaagtt tttcgaaagt atcgagacta
    15901 cacgatgatt tgcggcggag tcctgtcaga tgatcttgct gtacatgcat tgaagaataa
    15961 agaaaaaata ttggcgcgaa acaaaaagat cattacagag aaccttgtga ttttaaaaca
    16021 atgggtagcc aatgaaccga aagttgaact tgtcgctccc aattacgtct ctacttcttt
    16081 cattaaatta gccatcgaag aagatgacca gacattttgt atcaatttat tagaagaaac
    16141 aggagtacta ttagtaccag gaagtgcctt tgatttaccg aagcatgccc gactaggtta
    16201 ctgttgtaaa aaagaaacct tggaaaaggg attattcctc ttatctgagt tcttgaaaaa
    16261 acaggcctaa acaaaaacac tgcaaggaca gtctgtgaca actgttcaag cagtgttttt
    16321 catttacgct tattgtctat gagaaggaga gtgccctgaa gctatggaga ctcctgaagc
    16381 tttttcttct gcagtttctt gctgatggat ctcttgtgta ttgtgtcgta caagcaagaa
    16441 gttgaccaaa acaagaagag aagtcgaaag aaagacgccg cgataaccaa atgcactgga
    16501 aacactggag ccgatcatag gccctactac gttaccagtt gcttggaaag attggttata
    16561 actgaagatc cgtccagcag cttgatgagg cgaatatttt gtgatcaatg cttggacagc
    16621 agggagcaga caagcatcag agataccaat caaaaaacgt aaggcagcta attgccaaac
    16681 attcgtgacg aatgccattg ggatataaac cagaatcgca aaacccaaac caatcgcaag
    16741 tattctttca ctcccaatct tgtcacctaa gcgaccaaat cttggagcgg ctatcaaagt
    16801 ggcaatccca ggtatcgaag caatgattcc gctgatcaac gtaacattcc catgtccatg
    16861 cagcaattgg cgaatgtaca gactgatgat tggactaatg gaattgttgg atgcttggat
    16921 gatcatcgtt gtgacgaaca taccgatgac gacgtgtgga tatttcaagt ctttgaaaat
    16981 ctgtttggca gaaaccatgt tttctttttt cactgggaca aatttttcat ggacgaaaaa
    17041 cagactcaac aagaagacga aaaataagat gatccctgta atgaaaaagg tagggcgata
    17101 gccaaacgct gaagcagaga tcccgccaag aagcggtcca agtaatgttc ctgtaacaga
    17161 accagttgct aaggttccaa gaacttgacc acttttttct tttggcgtcc cagttgcgac
    17221 aagggcagtg gcattactga tataaccaga aaaaatccct tgaagcagac gcaatgcgac
    17281 taattgatac acactagtca cgcaacccat caaagaaatg acgattgcca ttcctaatga
    17341 agcacgcaac agcatcaatt ttcttccttt ttggtcggct aattttcccc accaaggaga
    17401 gataagcgta gtcaccaaga aagtagatga aaaagtcaat ccgctccaaa agttcaattg
    17461 tgacgtacta taattaccta acgtatctat gtaaagtgac ataaaaggca tgactaaact
    17521 aaatccgata ccagccataa atgttccaaa ccataagaca aacaagtttt gttcccaagt
    17581 ttctcttttc ctaaataatt tttcctttat tcttgcagcc aattagactc cttctttctg
    17641 aaaattttca tctacagctc tttattataa actacaatat gtagtttatc aatgaaagtc
    17701 tcttttttct ctttcttttt cgtggtaaac tgaaagagtg aaggatttat tttatgagag
    17761 gaaacatgga aatgaaaaaa agaaatctgt ctcaagagaa aatcatcgac tgctttcgag
    17821 aactagcaga agagatggat gttcagcaaa taacctttca gcatttagcc aaacgcttgg
    17881 atatcaaggc tccctcgctg tataaccatt tcaaaaatat tcgtgatgtc aaaacagcgc
    17941 taacagcgaa acttctgcag gaacttaatg accagctgcg acgggctctt gtgggaaaaa
    18001 gtggtcagga agcactgcag atctatgcag aaacttatcg atcctttgct ttttcaaatc
    18061 aagcagtgta tgagttactg atcagtgtac cgcataccaa tgaagctgtt ttattagatg
    18121 gaatccatga aacgaatcag atcattttgc agatatttga ggcttttcaa ttaacgaaag
    18181 aagaaaaagt tcatcgaagc agggaacttc gtagtatgat ccacggttat ctttccttac
    18241 gttttttagg atactttaca aaagaaccaa caatcgatcc agaagacagc tatttttgga
    18301 tgatcaataa ctttatcgct actttgcctt aaacaatcag attcatttgt aacaatttta
    18361 agatgttcat taaatgaccc ttttataaga gaaaattctt gcaactttct tgaaaagtga
    18421 ctaagatgag tctagaaaag ctgaaagaag gagcacgagt atgtatcaag taattaccat
    18481 gttcggggac aatgagcctt ggtggttttt tgaagactgg gaagaagata ttgaagaaga
    18541 agaaacattc gagtctttcg aagaagcacg acaagcgtac gaaaaacaat ggaacgaaat
    18601 ccatcaaaat tatgaatata tcaatgcaaa aagtaacttt ctcagcgcct tttggaatga
    18661 cggagatgag cgctggtgtg aagagtgtga cgatgattta cagcaataca agggacttgc
    18721 gttgttgaaa aatcatcagc ccatcacagt agaaagcaga aaggaatttt atgaaacaac
    18781 taattctagc ggaaaaacca agcgttgcga aagacctaag caaggtgctt ggtgctaatc
    18841 aaaagcacaa aaattattat gaaggaccca aggtgatcgt tacttgggcc cttggtcatt
    18901 tattaggatt gaaaatgccg gaagatctga ataaggaatg gcagagctgg caaatggaca
    18961 cgttgccaat gatccctaaa aaactaggaa tcaaaccact tcctaaaaca ggacatcaat
    19021 taaaagcaat caagcagctg gctcagcgaa aagatgtctc agaagcagtc attgcgacag
    19081 atgccggaag agaaggggaa cttgttgctc gctggatctt agagtatgtg catttcaata
    19141 aaccagtgaa gcggttatgg atctcttctc aaacagataa agcaatcaaa actggattca
    19201 agcaactaag acctgcgaaa gaatatgatg cattgtatga atctgctctt gcccgggcaa
    19261 aagcggactg gctggtagga ttgaatgtga ccagagcctt aactgtgaag tacgcggata
    19321 atttatcagc aggccgtgtg cagacaccga cactagcatt agtgagagac caagaaaaga
    19381 aaatcgagca gttccgtcca caaacgtatt tcacgatcac gttgcaagct ggaaaagaac
    19441 aagccaaaat gcttcaaaaa aatcaatttg ctttaaaaac acaagaagaa gcacaaaaac
    19501 ttgttcaatt gatcagtaca agcaaaggaa ctgtcacaga agtccaagaa aaattaaaaa
    19561 cggaatctgc gccattgcct tatgatttaa cagagatcca aagagaagcc aatcaacgct
    19621 atggctattc tgcaaagaaa acattaagtc ttgtacaaag tttgtatgag acccataaga
    19681 tcgttactta tccgcgtaca gacagcaaat atctgacaaa tgacatgaaa agtacgatga
    19741 aagaacgatt gcaagcagtc agcgactttg ctcctgaggt caagcagtat ttgaaaaacg
    19801 gagccatcgt ccaacagaca aaagtattcc aagacagtaa agtgaccgat caccatgcac
    19861 ttttgccaac tgaaaacaga gcacgttatg aaaaattaag taatgaagag cgaaaaatct
    19921 atcaaatgat tgtctctcgt tttttaggac tgtttgctca accccataaa gtcagccaaa
    19981 caaaagttac ggttgaattt ggtaaagaac gattcatttt tcggcaaaat cgtgtcgtcc
    20041 aagcaggatg gaaaggcgaa actgaaactg agacggaaac agtcaaatgg gaaaaaggaa
    20101 tgagaatcaa tcccgatttc gccatcaaaa aagaactatc tgcaccgcct aaaccattgt
    20161 cagaagcaag cttgcttggt caaatggaaa aatacagtct gggaacccca gctacgcgtg
    20221 cagagattat cgagaaacta atcaaatctg aactgatgga acgtacccct caaggactgc
    20281 aagtatctcc taaaggtaag caattattag tattggtaaa tccctcttta gtaacaccag
    20341 atttgacaga aagctgggaa aaagaccttg aagctattgc tgcaggaaag aaacaagcag
    20401 gaaccttctt gaatggaatc gaaaaagaaa caaaacgctt ggtgaatgag atcaaatcaa
    20461 gcaaacaaga atatcaagat ttctcgatta cccaaaagaa atgcccagaa tgtggggcca
    20521 atctgcgcga gaaaaacaca cgtgacggca aaatctatgt ctgcaccaat tcagattgca
    20581 actaccgcag aagaaaagat ccaaaagtct ctaatcatcg atgcccgcaa tgtcaccgga
    20641 aaatggaaat catcgaaggt aaaaacggtg cttatttccg ttgtaaatac gatggaacga
    20701 cagaaaaatt gcttgataaa aaagagcaga agaagaaaat gaccaaacat gaagaacgac
    20761 gcttgctgaa aaaatatgca caagcagaag aaccagaaga aagcccatta gcagcagcac
    20821 taaaagcagc aatgaagaag taataagata tgaaagaggt tgtgatagaa gtggtcagct
    20881 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt
    20941 atttccgaag aagctacttc tggaacacca tttattcgga aaaaaggact gtgacaagac
    21001 ttttgtcaca gtcctttttc atttaaaaaa atgtttactt catttgacgt atattattgg
    21061 tgaggcttca attcatttgg cttcactttt tctgtttctc ttatgatata aacaggacgc
    21121 tgtttcgttt ctaagaagat tttcccaata tatttcccga tgattccaag acataacaat
    21181 tggattccac caatgaacag gaagatcgag accatggaag gccatcctga tgtaggatca
    21241 ccaaacaaca atgcccgcaa aataatgaac aaaattgcaa taccagaacc gacacaagac
    21301 aaagcgccga taaatgaagc aatcgttaac ggtgcatccg agaagttcac gataccatca
    21361 atcgagtagt tgaataattt ccagaaggac caagatgttt cgccagccac acgttcgcgg
    21421 ttttcataag aaatatattc tgttttaaat ccgacccagc taaataatcc ttttgaaaat
    21481 cggttgtatt ctgttaattc taggacggca tccaccattt gacgagtcat caagcggaaa
    21541 tcacgagcgc catcgaccat ttctgtatcg ctgattttat tgatcaactg gtagaatttt
    21601 ttcgcaaaga aactgcgtat cgcaggctcg ccttcacgag tcgaacgccg ggtgccgaca
    21661 caatcgatat cagaagtagt gatccggttg atcatctcag gaagcagttc gggcggatct
    21721 tgcagatcaa catccatcac ggtcactaaa tctcctgtag aagcttgcaa tccagcgtac
    21781 agagccgctt ctttcccaaa atttctggaa aatgagacaa agcggacata aggataagct
    21841 gaggctagct tcttgattgc tgctaacgta ccgtcttttg atccgtcgtc aataaacaaa
    21901 tattcgaact catgggcacc tttgaatttt tcaacctcat caaaaaaaag aggaacagtt
    21961 ttttcttcat taaaacatgg gacaacaatt gatactttca tcgtttctat cctttcaatc
    22021 gtatgtttat atatggttta cattcaaaat ttaataaacc ttaaataaca cctttaaaat
    22081 ttgattttac gtaaaagaac aggtattctt tttgtaaact aacgtattta gtttaccatg
    22141 tttcctcggt ttatcaaact tttaagaaca atttttatta gttggcaagt agttttggat
    22201 agagaaaaag gaggaagttc cagtctattg actaggagca aacaaaatga aaatacacac
    22261 acctatcggc tttttatggt tagatgcaga tgaatccgga ttgactgctg tatcttttca
    22321 gccaatttca gaaagcgaat cagcgaatca gcgaatttta acgcgcacac aagaacagtt
    22381 tgaagaatat tttgcgggaa aaagaaaaga atttgatgtt cctttatcta taaagagagg
    22441 tacgcctttt cagcaaaatg tctggaaagc tttgtcgacc attccttatg gagaaatccg
    22501 aacgtataaa gaagtagccg ttgctgttga tagtccgaaa gctgtgcgag cgattgggca
    22561 ggcaaatcga accaatccat tgccaatcgt tattccttgt catcgagtga tcgggcaaaa
    22621 tggaaaactg acaggatata tgggaaacgc aatagatggg gttgaaatca aacgccaatt
    22681 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
    22741 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
    22801 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
    22861 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
    22921 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
    22981 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
    23041 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
    23101 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
    23161 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
    23221 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
    23281 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
    23341 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
    23401 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
    23461 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
    23521 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
    23581 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
    23641 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
    23701 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
    23761 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
    23821 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
    23881 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
    23941 aacggtgttc cagaagtagc ttctacggaa ataagccga
//
LOCUS       DAZKOK010000033        23642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000033 DAZKOK010000000
VERSION     DAZKOK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.033"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG90W_001159"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG90W_001159"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0477535.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG90W_001160"
     CDS             complement(1171..1866)
                     /locus_tag="ACG90W_001160"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0477536.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG90W_001161"
     CDS             complement(1879..2595)
                     /locus_tag="ACG90W_001161"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0477537.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG90W_001162"
     CDS             2925..3533
                     /locus_tag="ACG90W_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0477538.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG90W_001163"
     CDS             3858..4181
                     /locus_tag="ACG90W_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0477539.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG90W_001164"
     CDS             4363..5733
                     /locus_tag="ACG90W_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0477540.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG90W_001165"
     CDS             complement(5863..7164)
                     /locus_tag="ACG90W_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0477541.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG90W_001166"
     CDS             complement(7518..9509)
                     /locus_tag="ACG90W_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0477542.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG90W_001167"
     CDS             complement(9653..9919)
                     /locus_tag="ACG90W_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0477543.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG90W_001168"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG90W_001168"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0477544.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG90W_001169"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG90W_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0477545.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG90W_001170"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG90W_001170"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0477546.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG90W_001171"
     CDS             complement(14079..14600)
                     /locus_tag="ACG90W_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0477547.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG90W_001172"
     CDS             complement(14603..15445)
                     /locus_tag="ACG90W_001172"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0477548.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG90W_001173"
     CDS             complement(15438..16163)
                     /locus_tag="ACG90W_001173"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0477549.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG90W_001174"
     CDS             16339..16635
                     /locus_tag="ACG90W_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477550.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17203..18795
                     /locus_tag="ACG90W_001175"
     CDS             17203..18795
                     /locus_tag="ACG90W_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0477551.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="ACG90W_001176"
     CDS             18808..19548
                     /locus_tag="ACG90W_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477552.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="ACG90W_001177"
     CDS             19716..21065
                     /locus_tag="ACG90W_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0477553.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="ACG90W_001178"
     CDS             complement(21193..21783)
                     /locus_tag="ACG90W_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0477554.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG90W_001179"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG90W_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0477555.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4761 c         4502 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aa
//
LOCUS       DAZKOK010000034        23596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000034 DAZKOK010000000
VERSION     DAZKOK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.034"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..887
                     /gene="purR"
                     /locus_tag="ACG90W_001180"
     CDS             69..887
                     /gene="purR"
                     /locus_tag="ACG90W_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0477556.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1390..2763
                     /gene="glmU"
                     /locus_tag="ACG90W_001181"
     CDS             1390..2763
                     /gene="glmU"
                     /locus_tag="ACG90W_001181"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0477557.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2857..3828
                     /locus_tag="ACG90W_001182"
     CDS             2857..3828
                     /locus_tag="ACG90W_001182"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0477558.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3834..3936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4002..4571
                     /gene="thiT"
                     /locus_tag="ACG90W_001183"
     CDS             4002..4571
                     /gene="thiT"
                     /locus_tag="ACG90W_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0477559.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4652..5425)
                     /locus_tag="ACG90W_001184"
     CDS             complement(4652..5425)
                     /locus_tag="ACG90W_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477560.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5692..7392
                     /locus_tag="ACG90W_001185"
     CDS             5692..7392
                     /locus_tag="ACG90W_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0477561.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7362..7580
                     /locus_tag="ACG90W_001186"
     CDS             7362..7580
                     /locus_tag="ACG90W_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0477562.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7600..8490
                     /locus_tag="ACG90W_001187"
     CDS             7600..8490
                     /locus_tag="ACG90W_001187"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0477563.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8508..8792
                     /locus_tag="ACG90W_001188"
     CDS             8508..8792
                     /locus_tag="ACG90W_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477564.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9279..9893
                     /gene="gmk"
                     /locus_tag="ACG90W_001189"
     CDS             9279..9893
                     /gene="gmk"
                     /locus_tag="ACG90W_001189"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0477565.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9898..10203
                     /gene="rpoZ"
                     /locus_tag="ACG90W_001190"
     CDS             9898..10203
                     /gene="rpoZ"
                     /locus_tag="ACG90W_001190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0477566.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10456..12864
                     /gene="priA"
                     /locus_tag="ACG90W_001191"
     CDS             10456..12864
                     /gene="priA"
                     /locus_tag="ACG90W_001191"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0477567.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12879..13370
                     /gene="def"
                     /locus_tag="ACG90W_001192"
     CDS             12879..13370
                     /gene="def"
                     /locus_tag="ACG90W_001192"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0477568.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13363..14301
                     /gene="fmt"
                     /locus_tag="ACG90W_001193"
     CDS             13363..14301
                     /gene="fmt"
                     /locus_tag="ACG90W_001193"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0477569.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14301..15659
                     /gene="rsmB"
                     /locus_tag="ACG90W_001194"
     CDS             14301..15659
                     /gene="rsmB"
                     /locus_tag="ACG90W_001194"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0477570.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15672..16412
                     /locus_tag="ACG90W_001195"
     CDS             15672..16412
                     /locus_tag="ACG90W_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0477571.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16409..18478
                     /gene="pknB"
                     /locus_tag="ACG90W_001196"
     CDS             16409..18478
                     /gene="pknB"
                     /locus_tag="ACG90W_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0477572.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18643..19542
                     /gene="rsgA"
                     /locus_tag="ACG90W_001197"
     CDS             18643..19542
                     /gene="rsgA"
                     /locus_tag="ACG90W_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0477573.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19555..20205
                     /gene="rpe"
                     /locus_tag="ACG90W_001198"
     CDS             19555..20205
                     /gene="rpe"
                     /locus_tag="ACG90W_001198"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0477574.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20206..20844
                     /locus_tag="ACG90W_001199"
     CDS             20206..20844
                     /locus_tag="ACG90W_001199"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0477575.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20999..21853
                     /gene="mreC"
                     /locus_tag="ACG90W_001200"
     CDS             20999..21853
                     /gene="mreC"
                     /locus_tag="ACG90W_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0477576.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21857..22366
                     /gene="mreD"
                     /locus_tag="ACG90W_001201"
     CDS             21857..22366
                     /gene="mreD"
                     /locus_tag="ACG90W_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0477577.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22621..>23596
                     /locus_tag="ACG90W_001202"
     CDS             22621..>23596
                     /locus_tag="ACG90W_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0477578.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         7943 a         4250 c         5071 g         6332 t
ORIGIN      
        1 gctcgaattt ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt
       61 tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga
      121 atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat
      181 cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa
      241 ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg
      301 aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc
      361 ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag
      421 ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag
      481 ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta
      541 ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg
      601 gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac
      661 gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct
      721 tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca
      781 acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc
      841 aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa
      901 agccgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca
      961 taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa
     1021 atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc
     1081 cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta
     1141 agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt
     1201 atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata
     1261 ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact
     1321 tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga
     1381 gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg
     1441 aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc
     1501 attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca
     1561 gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg
     1621 ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg
     1681 ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa
     1741 tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca
     1801 ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa
     1861 gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac
     1921 aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac
     1981 tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa
     2041 acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat
     2101 gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat
     2161 tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca
     2221 ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca
     2281 aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc
     2341 agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa
     2401 attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa
     2461 ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat
     2521 gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc
     2581 ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa
     2641 aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca
     2701 attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac
     2761 taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag
     2821 aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca
     2881 agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca
     2941 gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta
     3001 aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca
     3061 gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca
     3121 aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt
     3181 gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca
     3241 agaatgctga ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc
     3301 gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc
     3361 gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg
     3421 gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca
     3481 gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg
     3541 atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact
     3601 agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa
     3661 gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa
     3721 atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc
     3781 catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca
     3841 caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc
     3901 ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt
     3961 cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt
     4021 gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga
     4081 tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa
     4141 gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg
     4201 gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt
     4261 ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata
     4321 cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt
     4381 tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc
     4441 cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga
     4501 tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac
     4561 aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg
     4621 tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg
     4681 catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt
     4741 cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg
     4801 ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct
     4861 gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt
     4921 ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt aatttactag
     4981 caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa
     5041 gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt
     5101 tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg
     5161 ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg
     5221 aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat
     5281 attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac
     5341 tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa
     5401 tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt
     5461 attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa
     5521 tcacgttttc attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt
     5581 tatttaaagg aggttttatg aattgttttc ttatggaatc tacaaagtcg aatacgagac
     5641 tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc gatggagata
     5701 aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca agcaaaacaa
     5761 ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg ttctgcaaaa
     5821 aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac tattgctaaa
     5881 agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt tgaatggaaa
     5941 aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa ctgttgtttt
     6001 ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa cggctatcaa
     6061 ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt aggtggagga
     6121 ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca tgatatcaac
     6181 tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat tttacaagga
     6241 gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt gcttgcgtta
     6301 ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag acgccaaatg
     6361 acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa gatggtcaaa
     6421 gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata tacggtgtct
     6481 actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa aacagaagaa
     6541 ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta tcgaaagatc
     6601 ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga tattgcgatt
     6661 aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc agcgaaaaaa
     6721 attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac actaggcagc
     6781 tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta tctggaaatg
     6841 aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt taaacctgca
     6901 agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt agaattatct
     6961 tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa cagggtcgta
     7021 ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt tttactgccc
     7081 aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga tataaaaagt
     7141 atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg gcgaaggtat
     7201 cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt tgattatctg
     7261 ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc agttgacaca
     7321 ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa gaattttcat
     7381 atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg cgtaaagaac
     7441 tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat tgggcgccag
     7501 accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa caactgcaaa
     7561 aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat gactggtttt
     7621 ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa aagtgtcaac
     7681 cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt tgaaaacgat
     7741 tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt tgtgaatcta
     7801 gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat tgattcactt
     7861 atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca agtgtcaatt
     7921 agtcggatgg cggaacgctt cgtgctggac gaaaacttcg taacggtaga agaaaaaccg
     7981 gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag cgcacttgat
     8041 tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt gaaaaagaat
     8101 actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat ctatgaaaag
     8161 gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa agtcgatgag
     8221 caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca cgaggaactg
     8281 gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca atgtcctgta
     8341 ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac gatcggttcc
     8401 aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat cttagaaaaa
     8461 atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg aaatattatg
     8521 acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc ccttctgaaa
     8581 aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac gaactgttta
     8641 ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt cgaattgaca
     8701 cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag atcatgggtg
     8761 tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag aaatacaata
     8821 aataaacaga attggatttc ttcgcttagc ttctttgctc taggaataca tttaaaggtt
     8881 gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc aaaaaccatt
     8941 ttctcggtat taagccaaat ttatgcaaaa atgtgagaaa ctattttcac aaaatttttc
     9001 ttcatactcg aaactaatcg gcttcttcac gacctcttat acacggcgtt caaaagcttg
     9061 catctgcgat aataacccca accaaacgaa aaacatgaga agctgtttat gtttggctgt
     9121 tcttattact tgatgcagga cagctttttt acgacctctt ggtacagggt agctttttta
     9181 ccaatttcta gctataatta aagataacct tgaaagttgc agaaaaaaag tgcaaaataa
     9241 acgataagta caaaaagact agttgaaagg acgtcgtcat gtcagagcgt ggattattaa
     9301 tcgtgttgtc tggtccatcc ggtgtaggaa aaggcacagt acgaaaagca atttttgaga
     9361 gtgaggataa tgattttcag tattcgatct caatgactac ccgtaaaatg cgtgaagggg
     9421 aggtcgaagg cgttgattat ttcttccgaa gcaaagaaga gtttgaatca atgatcgaag
     9481 ctggagagat gctggaatac gcagaatatg tcgggaatta ttatggaact cctttgtcat
     9541 atgtcaacaa gacattggat gaaggaaaag atgttttctt ggagattgaa gtccaaggag
     9601 caaaacaagt caaagaaaaa gtgcccgatg gtgtattcat ctttttgact ccgccagatt
     9661 tagcggaatt aaaatcacgg atcgtaggcc gaggaacaga tgcagatgaa gtaatcgaaa
     9721 aacgaatgaa agttgcaaaa gaagaaatcg aaatgatggc tctttacgac tatgctgttg
     9781 taaatgatga agtgccgcta gcagttgaac gaatcaaaaa cattattgcc agtgaacatt
     9841 ttcgagtaga tcgcgtaatc ggtaaatata ttaaaatgtt aaaggagatg tagtcttatg
     9901 atgttaaaac cttcaattga ctcactatta gatcgtgtaa attcaaaata ttctctagtg
     9961 attttagcaa gtaaacgcgc gcatgaattg gatgcaagtg cacagcctac tctagattct
    10021 tttgattcag taaaaagcgt cggtcaagct ttagaagaaa tcgatgcagg aaatgtagtc
    10081 aatgatccac atcctgaatt aaaacgtgaa cgtttgaaga tggaagaaga ggaacgtcaa
    10141 gcacaaaaag agcgtgaaca acacgaattg gaatcacgta tccgtgaaga gcaaaaaatg
    10201 taataaagaa aaatagagcg aacatctaaa aagaaaagca aacccagctg tttccttttc
    10261 aaacattcgc cacttattgt ctggtagaaa aggctgtgtc tgaacttttt gacacagttc
    10321 ttttttttct tatctgctgt taattttttg ttaggatttg gcagaagata agacaaatcc
    10381 atcgatctct agtaaaatag gcaaggaact ttttgtcgct tgataagaaa agacaaaaaa
    10441 aggaggaaac gaaccatgac attaacagct gaagtcattg tggacgtacc tactatgcag
    10501 accgatcagc cattcactta tttggttccc tctgaaatgg aaacagcttt gcaagtcggg
    10561 atgcgagtag aagtcccttt tggtaatggc aatcgtcatg tccaaggatt tgttatggct
    10621 atccaaaaat cagaagaatc agctaatcca tctttgaagg cagttattcg tttgctagac
    10681 cttgctccgg tggtcaatga agaattactt tcattagctg attatatgaa aaaaattact
    10741 tacgcattca aaatcacttg tctacaaacg atgcttccga gtgtgatgaa agcagaatat
    10801 gataaattga tctatccttt ggcagacaca ccagaagttc aggtattgtt tgatcagcga
    10861 gaagtgattt cctggaagga agcagaagaa gaaggcagcc tttctcaatt gattcgctgg
    10921 cgccaagagc aattagtaga tatcaaatat gaagtccata cacgaaataa agtaaaaacg
    10981 attcgtttgg tccgttcttt gttaactgaa aagcaaatcg aagaagaatg ggcaaaatta
    11041 cggcaaaatg ccaagaaaca aaaagaactt cttttgtgtt tatctgaact gagccaagaa
    11101 gaaccaattg cttatttcaa aaataaagga atcagtactg ctgtcttgaa tcaaggaaaa
    11161 gaaaagggct ggctggagtt tatagaatca gaacgttatc gcgaccctta taaagaccga
    11221 gtatttgacc aaacgacggc attggaactg aatgcagaac aaaaaaatgc tgtcgaacaa
    11281 atcatcacag caggtcagca gcaaaaagat caggtctttt tgctggaggg aatcactggc
    11341 agcgggaaaa cagaagtata tcttcaggca atcgctgatg ttttgtcaga aaataaaacg
    11401 gctattatgt tagtgcctga aatcgcattg actccgcaaa tggtcgagcg attcaaagga
    11461 cgcttcggag aatcagttgc ggtcttacat agcggacttt cccaaggtga aaaatatgat
    11521 gaatggcgca agattgagcg ggaagaagcg caagtagtag taggcgcacg atctgctatt
    11581 tttgccccgc tgaaaaatat cgggctgatc attatcgatg aagaacatga atcaagttac
    11641 aaacaagatg agacaccgcg ttatcatgcg agagatctag caatttggcg cagtaaatat
    11701 catcattgcc caatagttct aggaagcgcg acgccctctt tagaatcaag agcacgagct
    11761 caaaaaggcg tttatcagtt attgcagtta aaccaccgag ccaaagcagc tgcacagctt
    11821 ccagctattg agatcgtaga tatgcgtgaa gagtttcaga atcatcggac ctctactttt
    11881 tctgctaatt tgcaggaaaa aatacaaaat cgattagaca aaaaagaaca aactgtccta
    11941 ctgctcaatc gtcgaggata ttcgtctttt gtgatgtgta gggactgtgg atttgttctt
    12001 ccatgtccta attgcgatat ctctcttaca ctgcatatgg atacacgttc aatgcgctgc
    12061 cattattgcg gacatgaaga acctattccc aatcgctgtc ctaattgtgg cggcaataaa
    12121 atccgctact atggaacagg tactcaaaaa gtagaagagg aactccgaga actttatccg
    12181 caagctagga ttttgcgtat ggatgtagat accactcgaa gaaaaggggc acacgaacag
    12241 attttacaaa aattcggtgc aaaagaagca gatatattat tagggacaca aatgattgct
    12301 aaaggtttgg actttccaga agtgacatta gttggggtgt tgaatgctga tacctccctg
    12361 aatttaccgg attttcgttc tagtgagcat acattccaat tgcttaccca agtttcagga
    12421 cgagcagggc gagcggaaaa agcaggcgaa gtcgtgatcc aaacattcaa tcctcaacat
    12481 tacgccatag aattggctaa aaaacaaaat tatgagcagt tttatcagca ggaaatgcat
    12541 gtacggcatc gtggaggcta tcctccttat tactttactg tgaaaataac cgccagccat
    12601 cctgaagaac aagtagcggc aaagaaaatc tttcagattg ccaatcagct aaaagaagtg
    12661 ttaagccctc aaagcttgct attagggcca acgcccagca tgattttgag agtgaaaaac
    12721 cgatattatt atcaactaat catcaaatat aaacatgaac cgaatcttcc tcatgttttg
    12781 gatgaaattt tgaatggaag ccagaaagaa cagcgacaag gtctttttgt ggcaattgac
    12841 aacgaaccat taaactttat ttaagaaagg gccaaattat gcgctatcct atattgatcc
    12901 accctaatga taaattaaaa agaactgctc agccaatcga tgtaatcacg gacgagacaa
    12961 tcgctttatt agacaattta tatgagacaa tgattgcgaa tgacggaata gggatcgctg
    13021 ctcctcaagt aggacaaaac aagagaattg ctgtgatcga agtagatgaa ggcgagaaat
    13081 ttgaactgat taacccagaa atcatcgaag caaaaggtga aagccttgac gtagaaggtt
    13141 gtctcagtat tccgcatgtc tatggaacgg tcaagcgagc agatgaagtg accgtgcgtt
    13201 attatgatcg agacggagaa gagatagaag tgacggcttt tggctattta gcccgtgcct
    13261 tccagcatga aatcgatcac ttagacggta tcttgttcat tgagaaaatg atacagcaga
    13321 tcccggaaga agaacttgaa gaatatatgg aggaacatct tgatgactaa gctagtattt
    13381 atgggcacac ctgctttttc tgtgccgatt cttgaagggc tgatagaatc aggttacgag
    13441 atccaggcag tggtaacaca gcccgaccgt ccagttgggc ggaaacgagt aattacgccg
    13501 acaccggtaa aaaaagcggc tctgaagcac ggtatccgtg tgctgcagcc agagaaaatc
    13561 tccggttcgc cagaaatgga agaaatcatt gaattggtgc ctgatgtgat cgtcactgcg
    13621 gcatttggcc aattcttgcc tgaaaagtta ttgcaagtac caaaattagg ggcaatcaat
    13681 gtccatgctt ctttacttcc taaatatcgg ggaggtgcgc ctgttcatta tgccatcatg
    13741 aatggggaga aagaaactgg tgtaacgatc atggaaatga tcaaaaaaat ggatgcaggc
    13801 ggaatctatg cgcaagaaag cataccaatc actaaacaag atgatgtagg gacgatgttt
    13861 gaaaagctta gccttttagg aagaaagcta ttgctagaaa cattgcccaa tattttggat
    13921 ggacaaaaac cagtacctca agacgagtca gaagcaactt tttcgccaaa catcacacgg
    13981 gaacaagaag caatcgattg gcataaaact gcagaagaaa ttgacaacca agtacgcgga
    14041 atgcgtcctt ggccaattgc attcacgacc tatgagggca tacgctggaa attattatcg
    14101 gttgaagtat tggcagaaac tacagacaaa gcacctggta cgatcatccg gaaagacaaa
    14161 aaaaatctat ggattgcttg cggtaaacag accgtcttag ccatcaatga attacagcca
    14221 gctggtaaag gaaaacaggc cgtacatgat ttccttaacg gaagcggtca gcaagttcag
    14281 attggtcagc aggtgaaata atgaaaaaaa tccctaaaaa aatgaaaaaa tcagtacgat
    14341 atatggcatt actgacatta gagcgagtga acaagggtgg cgcctactcg aatcttttgt
    14401 tgaatgagat gatcaaacaa agcgagctaa atcaaaaaga tgtgggttta tttacagaat
    14461 tgatctatgg tacgattagc catcagcgac tattggaatt ttacgttgca ccattgatcg
    14521 ctaaagcaaa aaaagtggat gactgggtca agatgcttct ttatctttct gtataccaat
    14581 tagaattttt agataaagtc cctgcacatg ctattttaaa cgaggcggtc gaaatcgcaa
    14641 aagtaaaagg aaatcctggg acagggaaat ttgtaaatgg tgtcttgagg aattatcagc
    14701 gtcaaggagc accagatctg caaggcattg ctgatcctat cgaaagactt tctgtcaaaa
    14761 tcagcctgcc tctatggatg acgaaacgac tggtggatca aatcggatac gaagaaactg
    14821 aaaagctcgg tttatcgcta tatcagccta gccatgctag tgcccgagta gatacacgcc
    14881 gactttccag agatcaggcc atcactgttt tgcaagaaga agagatcaca gctgaaaaaa
    14941 gccaagtcag tccatacggt atagtagcga aaaaagggca tttagcttct agctcattgt
    15001 tccacgatgg cgtcatgacg atccaagatg aaagttcgat gcttgtagcg ccgtctatgc
    15061 aaatcgagcc tgaacatcat gtcttagatg cctgtgcagc acccggaggt aagacgacgc
    15121 atattgcttc ttttttagat ccgtcatgcg gtggagaagt ggtcgcttta gatgtacacg
    15181 aacacaaagt caaactcatt gaagataatg cagcacgtct aggtgtctct tctgctgtct
    15241 acgctcaaaa gatggatgct agagaagtca gtaaacagtt cgaagcagag cagtttgatc
    15301 ggattttagt agacgcgcct tgttcaggtc ttgggctgct tcggcgaaaa ccagatattc
    15361 gttataaaaa acagccggat gaattagcga atttaccaaa aatccaattg gaaattttag
    15421 aaagctgcgc accaacttta aagtcttccg gtattctgac ttatagcact tgtacgatct
    15481 taaaagaaga aaaccaagac gttgtttctg ctttcttgga aagacaccct gattttgaga
    15541 ggattgatgt tttgaccaat cctatgctac aatcatcaat cgaagagaaa atgttgactt
    15601 tgtatccgca tcagttttac acagatggat ttttcatttg ttgtttgcgc aaaaagtaat
    15661 gaggtgaaaa aatgcagatt gaatatcaat cagatgtagg aagacgacgt aacacaaatc
    15721 aagactatgc cagtgtattt accagccgtt caggtctcaa gcttgctgta ttagcagacg
    15781 ggatgggtgg tcatcgtgcc ggagacattg ccagtcagat ggcggtaacg aatctaggcg
    15841 aagcttggga aaaacaagaa gctgttgacg atgaaaaaat cgcccaatgg ttcattcaat
    15901 cgattcaaga agaaaatatg ctgatttatc agcgtggaca agaacagccg gaatataacg
    15961 gtatgggaac aacaatcgta gcggccgctc tgtcagatga ctggttcacg attgccaatg
    16021 ttggggatag cagagcttat ttgattcgtg aagataaaat catgcaattg acggaggatc
    16081 attctttagt caatgaacta gtcaaatcag gagaaatcag cgaagaaatg gcagtgaacc
    16141 atccaaggaa aaatatttta acacgttctg tcggcatgcc tggaacagtc gaagtagatg
    16201 tttctactca tctttggcaa ctaaaagatc gactgctgtt atgttcagat ggcttgacga
    16261 acatgctgtc tgaagaagaa ataggaacga tcatcaatca agaaggccca ttgtctcata
    16321 aagtaactga gctgattgat cgtgccaatg aagcaggcgg agcggataat atcaccgttc
    16381 ttcttatcga atataaggag gacagtgtat gatcgagttg ggcaaaaaat taaatggccg
    16441 ttatcaaatc actggaaata tcggtagcgg tggtatggca aatgtatttt tggcgcacga
    16501 tttaatcttg gatcgtgatg tagcagttaa agtcttacga tttgactttc aaaatgacca
    16561 gactgcgatt cgacgatttc agagagaagc tttagcagca acagaactcg ttcatccaaa
    16621 cattgttagt gtttacgatg ttggtgaaga agacaacatg cagtatctgg tgatggaata
    16681 cgtcaaagga atggacttga aacgatatat ccaaacacat tatcctgttc cttatgaaac
    16741 agcagtaaat attatgcagc agatcctttc tgctatttct ttagctcata accatcaaat
    16801 catccaccga gatttgaaac cccaaaacgt actgatcgat aatgaaggtg ttgtaaaaat
    16861 cacggatttc gggattgcta tcgctttatc tgaaacatcg atcacgcaga cgaatacgat
    16921 gctaggatct gttcattatc tgtcccctga gcaggccaga ggaagtatgg caacgaagca
    16981 gtcagatatt tatgctttag ggatcatcct ttatgagatg ttgacgggta gcgtaccgtt
    17041 tgatggtgaa tctgctgtaa caattgcttt aaaacatttt caagatgatc ttccatctat
    17101 aaaagcactt gatccaaatg tgccacaagc actggaaaac gtgattttac gggcaacggc
    17161 taaagaacct gcagaccgtt ataagtcagc cgaggaaatg tctgatgatc tttctacagt
    17221 attatcacca gctcgtgcaa acgaagaaaa atggcagcct catgtgatgg ataatgaaac
    17281 aaaagttatc acacccctta cggaagatgc accaatgcct gattcattca aatctatgcc
    17341 tctgccaaaa gacaagccag aaaacgaaac cttaccagag gaggaagaag ttctgcctga
    17401 tcctaaaaag aaaggacaga aaaaatggtg gctgatccta ttagctattc ttgctgtttt
    17461 aggaatcgga acagctgtat tctttgcttc tggcgggcga ggagaagttg ttgttcctga
    17521 tgtttctgat ctttctgaag cttctgctag agaaacactc gcaaaagcga aattagaagt
    17581 agcaacaaaa acagaagaaa ttgcagatga caaaatcgaa gaaggtaatg tagtgaagac
    17641 agacccggca gcgggaacga cagtcaagca aaaacgcgaa gtcactctgt atattagtag
    17701 cggcacgaaa aaaatcaaat tagatgatta cagtggtaaa agttatgaag atgccgaagc
    17761 aagtttgaaa gaattagggt tttctgctga cttgattcaa aaagaagagg aattcagttc
    17821 agaggtagaa agcggaatga tcatttcgca atctccatca gaagatacag aagtcgatcc
    17881 taaaacagac acgattactt tcacagtcag caaaggacca gaaacagtga ctttgagtag
    17941 tcatgtagga gaaaactacg aaactgttcg taattggctt gttacacaag gattcaatat
    18001 caataatatt agtgctactt atgactatag cgacactgta ccatcaggtt cgatcatcag
    18061 tcaggatcca gcctcgggtg aagttgtagc agaagaaact tacatcaatt ttgtcgtaag
    18121 ccaaggaaca gagcctaaat tgaatgatat ctccggctat acaaaaagcg aagcacaaag
    18181 ttatttagct agtgtcggtg cagaatatat cgggcatgag acttccgaat attcaaacac
    18241 ggtggataaa gataaagtga ttcgtacagt accaggggca ggaacgacaa ttaccaaagg
    18301 aatggttgtg aacgtaattt actctaaagg accagatcca gcgtcttcta gttctgaaaa
    18361 agactcttca gacagttcta gcagctcaag tagctccagt agcacaagca actcttcaaa
    18421 agaagaatct tccagctcca caagttcatc agctagcacc tcagaaagtt caaaataagt
    18481 gctgaataaa atatatcaga agggaatgcg acattgattt gttgcattct cttttttgtg
    18541 tttctatctc ttttgatcaa ttttcaagcc atatactttg agaagtttgc atggaataac
    18601 acctactttc ctcttcgtat ggtaaaataa acaaaaacgg aggtggcact tatcaaagga
    18661 caaatcagaa aagcaatcag cggtttttac tatgtatacg caaacggaga gacctatcaa
    18721 acaagaggaa gaggaaattt ccggaatcgg aaaatcacgc cgcttgtcgg agatcaggtg
    18781 atctttgaga gtgaaaatca aacagacggc tatcttctgg aaatcttgcc gagaaaaaat
    18841 caattagttc gcccaccagt cacaaatgtt gatcttggaa tcattgtcac aagtatcgta
    18901 gagcctgcat tttcttataa tctacttgac cgctttctag ttaccttgga atacgagtcg
    18961 atcgaaccga ttattttttt gacaaagacc gacttgtcag acgacccaga aaaaatcgaa
    19021 gagatcaagc aaacctatac taaaataggc tatccggtga ttgtacccaa tcatacgggt
    19081 gatacgacag aactgatccg ttacttccca gaacggctat cggtctttat gggtcagtct
    19141 ggagcaggaa aatcgacgct actgaatcaa atatcaccgg aactgaattt agcaacagga
    19201 gagatttctg atgcacttgg aagaggacgg catacaacac gtcatgtgga actgattcct
    19261 atctatggcg ggttagtagc agatacacca ggatttagct cgattgactt tttgacaatt
    19321 gaagcaggag aactgccgag acaatttcct gaatttgtag aagcttctgt ccattgcaaa
    19381 tttagagagt gcatgcatgc taaagaacct ggctgcgaag taaaacatag agtagaaaca
    19441 ggtggtatcg cacagacacg ttacgacaat tatttgcaat tcttacaaga aatcgaaaaa
    19501 agaaaaccaa tgtatgcaaa aaaagaaggt aaacgaaaat aggaggaaac aagaatgaag
    19561 atagcaccat ccattttgag tgcagatttt gccaatttac aaagagacat tgagttagtg
    19621 gaaaaaggag gcgcagatta catccacgta gatgtaatgg atggccgatt tgtcccaaat
    19681 atcacattag ggccaaatat tgttcaggca atccgtcctg ttactaaatt accgttagac
    19741 gtccatctga tgatcgttga tccagaaaac tacattccag catttgcaaa agctggagca
    19801 gatatcatta ctgtccacgt cgaagcaact cctcatattc atcgggcact tcaaatgaca
    19861 aaaagcttag gagttaaagc tggagtagtt atcaatccag gaacaccagt ctccatgatc
    19921 aaacatgttt tgcctttggc tgatcaagta ctagtcatga ctgtcaatcc tgggttcggc
    19981 ggacaaagct ttattgaaga aacagtagaa aaaattacgg aactcgctga tctacgtaga
    20041 gagaacaatt ggacttatgc aatcgaagta gatggcggaa tcgttccaga aacagcaaaa
    20101 atctgtaaag atgcaggggc ggatgtcttc gttgcaggat catatatcta tggagccgaa
    20161 aatccagtcg gacaaatcga acgattaaaa gaggcattag actaaatgaa aattttatta
    20221 gctgctggag gtaatccgca aaactggccg cagtctctgc tgtccaaata cgatgcctat
    20281 gtcgggatag accgtggtgc gctgtacctg cttcaagcag gctacccagt agatctggca
    20341 gtgggtgatt ttgattcatt gtcgaacgaa gagcagaagg aagtatttgc aaaagccaaa
    20401 gagtataccc gttcccctgc agaaaaagac gatacagata cacagttagg tatcttaaaa
    20461 acatttgaac gttacccgca agcggaagtc actttgattg gagctacggg cggaagattg
    20521 gatcatcttt tagccaatct ctggttaggt atggaacctc gcttcaaacc ctttgcccat
    20581 cagcttatta tcagagattg tcaaaattat ttgtcttatt atttgcctgg agagcatata
    20641 gtaagacaga taccagatat gaagtattta gcttattgct gcttgactcc cgtctcgaat
    20701 cttacattaa aaggcagcaa gtatgtattg gaaaataaag aagtagaata tccgacatct
    20761 tatgcaagta acgaatttct ttcagatacc gcagaatttt ctttttcaga tggtatgatt
    20821 gcagttattc aaagcaaaga ttgatccata aacaaataaa aaaatttgtc tttcaggcta
    20881 aaaatgacag aatctattat ttgctaaaaa aaagcttaag aggagcaact taccttttgt
    20941 ttcacggcat ttagtgataa aattgaacac aagagtctaa ttaattcgag gtgtctttgt
    21001 gaagaagttc aatcctaata aaaatattat tattaccttg gttattgtta tcatcgtggt
    21061 gacgatacta agtgtgaccg ctgcgcagcg agcgaatgaa ggaaagccca atttcttcca
    21121 atccatcgtt aatgacagtg ttgcaatggt tgataaaaca atctctgccc cagtacggtg
    21181 ggtacgaaat ggagtagatt ctgtccataa tcttttttca acttatgccg aaaatgagcg
    21241 gctaaaagaa aaaatcgata gctatgatga cgtttcacag caaaacaaga attacgagag
    21301 agaaatcgaa gcgttgaaaa aagaacttga gttgaatcag acgctgacta gttacgaaaa
    21361 agtaacggca aatgtcatta ctcgttcgcc tgacagttgg caggacatgc tgatcgtaga
    21421 caagggttct aaagacggta tcgaagcaaa tatggcagtc atgtcgcaaa aaggattgat
    21481 tggccgagtg atcgaggtca atacggcttc gtctaaaatc gaattactgt catcctctaa
    21541 tgaaagctcc aatcattttc cagtacgggt atcttcggct aatggcgaag cgtttggttt
    21601 gcttaaaaac tatgatgaaa agctccatgc cttagtggtg acccaattaa ctggtgatac
    21661 ggatatcaaa gaaggggatg ttgtccagac atccggtctt ggagggaatt ctccagctaa
    21721 cttgccgatc ggtacggtta ttaaaacgaa accagatagt tatgggctgg atcgggaagt
    21781 ttatgtgaaa ccttatgcag aaatgtatga cgtgtcagtt gtgacgattg tccaacggct
    21841 agcggaggat tagtccatgt tgaagaaaga aacaatgaag tactatctgc caatcgtttt
    21901 gttctttttg atgttgatag atggtcattt aacaagaatg ctaggggagt ggtcgaaagg
    21961 cacctatatg tcaaatgccc actttctgat attggcatta ttatgttgca gtatggcgtt
    22021 tgaaaaacgt tatttactga ttaccacgat tgttctcggg gctatctatg acgcttacta
    22081 tattggcgtt atcggtatct atgcagtagc tctcccttta attgtatggt tgatgtatgt
    22141 aatgaaagac gttatccatg tcaacatctt tactgaattt ttcagtatga tcatctttgt
    22201 cacgggttat gaattgttta cgatggtggt ccagttgatt tttaaattag cagtagtaaa
    22261 taacacgtat tttattacaa ggtttttagg acctacactg ctgttgaaca tgattatatt
    22321 tgtattattc atttttccct ttaagaaatt attcagtgat gaataatttc tttttttatt
    22381 tttaggtcga aagcgaaaaa gaatcgttgt tatcttaaga aatgctagac ttaacgcggc
    22441 tttttcatga tcattttctg aaacgaacag ttttgtcatt tttaaaagtt gttttgaaac
    22501 atttttgtaa cataactatt atatcgttga cacactgcta tggtacaatt tttatatcgt
    22561 taagaaagac ttaaatatat ttagatattt attaaattaa gccagtcgga ggaatgaaga
    22621 gtgaaaaaga gtttaatatc agcagtaatg gtatgttcaa tgaccttgac tgccgtagca
    22681 tcaccgatcg ctgccgcggc ggacgatttt gattctcaga tacaacagca agatcaaaag
    22741 attgcagact tgaaaaatca acaagcagat gcacaatcac aaatcgatgc cttagaaagc
    22801 caagtttctg aaatcaacac acaagcacaa gacctattag caaaacaaga tacattgcgt
    22861 caagaatctg cacaattagt aaaagacatt gcagatttac aagaacgtat tgaaaaacgt
    22921 gaagatacaa tccaaaaaca agcacgcgaa gcacaagtaa gcaacacaag ctcaaactac
    22981 atcgatgctg tattgaatgc tgattcatta gcagatgcta ttggacgcgt tcaagcaatg
    23041 acaacgatgg taaaagcaaa caacgacttg atggaacaac aaaaacaaga taaaaaagct
    23101 gtagaagaca aaaaagctga aaacgatgct aaactaaaag aattagctga aaaccaagct
    23161 gctttggaat ctcaaaaagg cgatctttta tctaaacaag ctgatttgaa cgtactgaaa
    23221 acttcattag ctgctgaaca agcaactgct gaagacaaga aagctgactt gaaccgtcaa
    23281 aaagcagaag ctgaagcaga gcaagctcgt attcgtgaac aacaacgtct agcagaacaa
    23341 gctcgtcaac aagctgcaca agaaaaagct gaaaaagaag cacgcgaaca agcagaggct
    23401 gaagcacaag ctactcaagc ttcatctact gcacaaagct cagcaacaga agaatcatca
    23461 gctacacaat cttcaatgac tgaagaatca tcatcagcta cacaatcttc agcaacagaa
    23521 gaatcaacaa cacctgaatc ttcaacagaa gaatcaacag cgcctgaatc ttcagcaaca
    23581 gaagaatcaa caacag
//
LOCUS       DAZKOK010000035        23215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000035 DAZKOK010000000
VERSION     DAZKOK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.035"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..329)
                     /locus_tag="ACG90W_001203"
     CDS             complement(<1..329)
                     /locus_tag="ACG90W_001203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477579.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(343..600)
                     /locus_tag="ACG90W_001204"
     CDS             complement(343..600)
                     /locus_tag="ACG90W_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477580.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(795..1337)
                     /locus_tag="ACG90W_001205"
     CDS             complement(795..1337)
                     /locus_tag="ACG90W_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0477581.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1358..2449)
                     /locus_tag="ACG90W_001206"
     CDS             complement(1358..2449)
                     /locus_tag="ACG90W_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0477582.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2457..4667)
                     /locus_tag="ACG90W_001207"
     CDS             complement(2457..4667)
                     /locus_tag="ACG90W_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0477583.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4683..7211)
                     /locus_tag="ACG90W_001208"
     CDS             complement(4683..7211)
                     /locus_tag="ACG90W_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0477584.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7263..7673)
                     /locus_tag="ACG90W_001209"
     CDS             complement(7263..7673)
                     /locus_tag="ACG90W_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0477585.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7686..7904)
                     /locus_tag="ACG90W_001210"
     CDS             complement(7686..7904)
                     /locus_tag="ACG90W_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0477586.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7901..8893)
                     /locus_tag="ACG90W_001211"
     CDS             complement(7901..8893)
                     /locus_tag="ACG90W_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0477587.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8972..9883)
                     /locus_tag="ACG90W_001212"
     CDS             complement(8972..9883)
                     /locus_tag="ACG90W_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0477588.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9902..10246)
                     /locus_tag="ACG90W_001213"
     CDS             complement(9902..10246)
                     /locus_tag="ACG90W_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477589.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10266..10544)
                     /locus_tag="ACG90W_001214"
     CDS             complement(10266..10544)
                     /locus_tag="ACG90W_001214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477590.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10903..11403)
                     /locus_tag="ACG90W_001215"
     CDS             complement(10903..11403)
                     /locus_tag="ACG90W_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477591.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11404..11949)
                     /locus_tag="ACG90W_001216"
     CDS             complement(11404..11949)
                     /locus_tag="ACG90W_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477592.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11946..13202)
                     /locus_tag="ACG90W_001217"
     CDS             complement(11946..13202)
                     /locus_tag="ACG90W_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0477593.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13563..13874)
                     /locus_tag="ACG90W_001218"
     CDS             complement(13563..13874)
                     /locus_tag="ACG90W_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477594.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13897..15372)
                     /locus_tag="ACG90W_001219"
     CDS             complement(13897..15372)
                     /locus_tag="ACG90W_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0477595.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15821..16273)
                     /locus_tag="ACG90W_001220"
     CDS             complement(15821..16273)
                     /locus_tag="ACG90W_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477596.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16275..16640)
                     /locus_tag="ACG90W_001221"
     CDS             complement(16275..16640)
                     /locus_tag="ACG90W_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477597.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16739..21022)
                     /locus_tag="ACG90W_001222"
     CDS             complement(16739..21022)
                     /locus_tag="ACG90W_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0477598.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21169..21408)
                     /locus_tag="ACG90W_001223"
     CDS             complement(21169..21408)
                     /locus_tag="ACG90W_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477599.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21498..21740)
                     /locus_tag="ACG90W_001224"
                     /pseudo
     CDS             complement(21498..21740)
                     /locus_tag="ACG90W_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21853..22104)
                     /locus_tag="ACG90W_001225"
     CDS             complement(21853..22104)
                     /locus_tag="ACG90W_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477600.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22267..>23215
                     /locus_tag="ACG90W_001226"
     CDS             22267..>23215
                     /locus_tag="ACG90W_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0477601.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLS"
BASE COUNT         6855 a         4992 c         3505 g         7863 t
ORIGIN      
        1 gaattcattc cccaaaggcc ttttaagatt gatacagcag tccgttcact tcttttagac
       61 ttgtcataca agtcttttaa atgatcccat atcagactag tttgtctatc tagcttcaat
      121 ccattaaacg ctaatgaacg gttcattttt tctattactt tagataaagc tacagaaatt
      181 tttatacctt cacaacgcgc atcttggatt tcaataatag tatcatcaat tagtttttga
      241 atttcttgcc tatcaacata ttcaatctta gaaagagaat cttttaaagc tactaatgat
      301 tcaacgattt cttttttctc ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc
      361 atgtagagca gagtccattg aaccagtaat tacactatct acaacgccat tccaatttac
      421 tctacatttt ccatttttca gatttgtcgg tccatacgga tagccttttt gacattcaat
      481 gttcggatta taccagatag gattatacca ttcagccgca gaagcagatt cagtcgatac
      541 aaacaatccc acaccaacaa atccaataca catcacacta tagccaatta gttttttcat
      601 ctcgtatcca tctcctaaaa aatagaatag ttcttatagt tattcataag acatattttt
      661 tccgtttttg ttttatcctt tctgtcctat aacaactttt ttcaagtaaa gaatgacata
      721 gtttttctta aaaaaacagt ctgttttttt ctatttcttc ggtgtataat aaatatgagt
      781 gttcattttt tattttaata cgcactccgt acataataaa tcttgatcgc atcaatcttt
      841 tgagcttctt gatctacttt caattgaagt aatacaaatt gagtaacctt attcattgtt
      901 tcttctccaa ataacaaata ttgatccgga ttctctatat cttgtgtcac ctggtaaagt
      961 ttcccagttg ttttcatttg tacatgcggg tctacatcaa tactattgtc tttaatacaa
     1021 cgatctgtca tatagtccct cacagcttga ttgcgtttat aaacatctgt gaaattactc
     1081 cattttctag caaaatcttt taagaaattt tttgtcattt catctatttg catttgttgc
     1141 gctgtctcgt cttctaatga accaatttga ctgttacttt cagtactttg aagagttcca
     1201 cttgtttctt ttactgcttc gattgtttct tctgtcgttc tcaattgtgt tttttgtttt
     1261 tcagaagaaa accaatgaca accagtaaga cctatcaaac ataaccaact aaatactaaa
     1321 attatccctt ttttcacgtt tctagtcact gcctttctta tttcacctta cgaaccagac
     1381 tacgaatttt tgtttgattg tacgtgcgtt gataccgagc cataatccgt ccttgctcgg
     1441 cattctgttc cacactagag aaatgttgtc cgccatatga aacatctgtt ataattccgg
     1501 tgtgaccata gattccagga gacaatgcac cacctgcttg ccaattcacc acatctcctg
     1561 cttgtaaatc acttggtttt gggttcatga ttacttgcca accataggtt gtccagttat
     1621 aatcttcccc tattaacatc gcaaacatgt gtccagaacc cattaactgt gggccaccta
     1681 attgagatac ataccatgcc gtaccaccat aacattcccc attatttacg gatcgtccaa
     1741 tgatccgatc caaaaccttc gcattgcctt tactttttcc tgcaataagc gaagtttttt
     1801 ttgttggtag gctattccaa tgcttgatcg caaaagtatc atattccgtt aagttatagg
     1861 acgttatgat actcattgct cctcgataat acggactccc ttcggtaata cctgctgccg
     1921 catagcctcc acgacctaga atcaataaac aacttccagg atctttattg cccacacatt
     1981 gattaaagtt gctttggctc gccaccataa agccataata gtcataacaa gcttttaagt
     2041 tcggaaactt catatagaaa ccaccttcag aaccaccaat tccacgtgca gaaccttgtg
     2101 ggtagggaca accaggaaac caggtaatcc caaaaaagtt cgtatcgttt cttccagatt
     2161 gtgactgtcc ataagctgat tcatagccta attgagaaag aatcacactc ggtaataact
     2221 gatactttac agctgcacca actgctgctt gcgcatgttc atctgtgatt ggaccacgtc
     2281 ctttaatctc tgaaaaaatc ggtaatcctt cggtatattc cccattaaaa gcgacagcta
     2341 gacctgtagc ttctcctcca tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa
     2401 aaaagggaag aataaagaaa agaagacaac ctagacaccc tttcttcatt tcttcctcat
     2461 cctttcttcg ataatggttt ttggactgtt cgtccttttg gtaaagtaaa atcagttcct
     2521 ggttttgcgt ccaaaatcgg cagtgtttta ggatcctttt ctattttttc cgattgctct
     2581 gttggcttat ctaaagaaga aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt
     2641 ttactttcag acgaacgttg ccccggttgt tgcacagaac gatttcctga tagagtggct
     2701 accgacgaat taggaacaga gtattggttc aataactgct gcattgctgg ataactccca
     2761 tttttcgcct gatttttcac ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat
     2821 tgtggcacgg gtacttttcc agaaggcaac ggaatcgatt gaccaacaaa agcgatacct
     2881 ttatttaaca atggttttct ttgattattc ccttgttgct tactcaattg tgccaatgat
     2941 tgtacctgtt gttttggtct tactgaattc atggacgaat ttggaatcaa accatttgaa
     3001 agaccagaat tccactctgt tttcgatttg gtagaagaca tttcaggctc taccccatga
     3061 taacgaacat gacgaccact ataaggcaag gcttgatttg aacgcccacc tagttcaaag
     3121 ggattaacca cttgcctccc ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt
     3181 tgaaatccat gtatcgctgc agaagcgagt ggattcgttg ctcgatacac attccctgct
     3241 aatcctgcac ctttgattgc caaattttgt ccagcagttg atagttgacc aggcagttgt
     3301 ttcatttgag acaaagaatg acttgctatg tttttcgtgt tgtttatagt ttgatctgca
     3361 gctcctaacc ctagtttctt taataaatct ttccgtttta caatgagtaa aattgatagg
     3421 agtgctttga caaacatatt tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg
     3481 tctactatgt tatagatcaa aaatgccacc aaaataatca cactgtaaaa agatttttga
     3541 aacatcaagc ccaaaaagga ctttaaggta ttcatgagca tataatcaaa tactggaaca
     3601 aaagaagcga gtaatgaaaa aggaatcaca agtaataaac acaacaccaa catttgaaag
     3661 ataaaacgaa tcatgccaat cactacaagt ggtaatccaa tacttagatt aagcaatggc
     3721 accatagaac caacgactgc tttataaaca gctttagttg gttgaatata gacacgataa
     3781 gccttctttt tccctctatc ttctcctgca gcagtggtca cttttgtctc aatatcagat
     3841 aattcttcac ttgacgcttc cttggctaga aattcttgtg gttcaatctc tgcttgatac
     3901 aattcttcta aatttgttgt cccataattt aacaatatat acggatcaac aacagccgct
     3961 ttataataaa gttggcgcac tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca
     4021 ccattgacat cttttgtcgc caattcatct agactatttg tagcagaaaa aataaagcct
     4081 tctacctcca cggacaaatc atttaaccct ttggtaaaag cttctccttt actaggtacg
     4141 ttactaccat agccaaacca aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac
     4201 accatttttg cgtaatgttt ccctctaaat atacgaagag taaatagata taacacatac
     4261 aagacgacaa tccccgcacc aataggatcg tatatttttg aataaatagt atccgaaaaa
     4321 gtgaaaaact ggtgaattaa ctcgttcatt gcagctcctt catagagctt ctcaatgaca
     4381 aaatcaaggc cttgccaaat caatttactt aaactaaaga acatattcgt catcattttt
     4441 agaaaatctg tgccatttgc cccaaataac attttgccta atgaaaattc actatcagat
     4501 aaataagaag aaaaggtatc aatatgaaat ttcccaatct catagatcga ttgatccacc
     4561 tgcttttctg ccgcatacac caatccacca acaaaaagca gtccgaatcc aatcgttaaa
     4621 aaaaacaaaa cgcaccattt cttttcctta tgctttatat aagccattgt ttcacctcat
     4681 ttctattgca tgattaacgt atcctgttct tctacttgag agtgacttgt tttttccgtt
     4741 gtttgtaatg cagcggtcat ttcttcaaac aaactgtgta cattgagttt tcctacacgt
     4801 ccatagggat ctaacatcca acactgtcct ttaatcatgt ggctcatttc agctaagttc
     4861 gcttcattta cttccaaacg taaatgattc aaaatctttt ctcgctcact gtcttcatca
     4921 aaagcaaaga cgacaccaaa attccctttt tctttatctg aacctaagtc accaataaat
     4981 tgtgtatcta acactaattg attcatctgg gaacgcccca cacgtgccat tgctgcaata
     5041 atactacgtc ctgcgttcga attattaaat acccatgctt ctgtaaaata aatcgctgtt
     5101 ttcttactac tatctcgttt tccaaacatt tcacaaaaac gacctaaact aatcatcaaa
     5161 cacaaagact ttcgatccgc atctgtatat agttctggtc ggacattgtc atttggtaat
     5221 ttcaaatctt ggatctctaa aatcgttgtt ttggcattaa agtctaatcc cgggttttct
     5281 ccataagaaa atcctaaacg taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca
     5341 tattctcgaa cctctttttg ttccatttgt tctaaatagt cgaccacatg catcaagcca
     5401 acttgttgtc cggcttcttt ttgaactaaa acttcttgga ttccctttaa taccgcccct
     5461 tttaaaaggt gattggtacc taacggactt aactcatcaa tcatatcctg ggctacttgt
     5521 ttcgcatcta ctcgggataa acaaacaatt ggatctaata caccataatt acggggattt
     5581 gtagcatcta acgtgacata actcattttt tgcaaatgtt ctacaaacaa agggtaatgt
     5641 ttctgataat acgggtcttg aatgagtttc gtaaaccatt tgcgcttttc tcctttggga
     5701 tccacaaata aactttgtac attcataaac gaactataca aaaagagcaa gccaactaaa
     5761 aaggtcttcc ctttaccggt ttttccagtt actgctacat gaggcgcatc aaacaacgca
     5821 cctttgattc cttccgcaat ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa
     5881 attaattctt ctaatggtgt tttttctcct cgagaaccac catcccctaa tgtatcttgc
     5941 cgaccaatat accagccgga ttccatacct aattctgata ctgctgaaaa caaccattcg
     6001 ccaactgctt ctacagttgt tcggtgcttc caatgtaact gccgtcccaa tggttgccct
     6061 aataaacatt tatagaacaa ttctcgttga tcattttttg cacggtggat atggataatc
     6121 gtatgaagtt gttgaataac cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg
     6181 tatacaataa aacatgcgat ccaatcgacg acaggttgcc cattatctac tcgttgcttt
     6241 aaatgtttta ctaatcctcg aacagttaat aagcgttcac taggttcttc atcattttga
     6301 accaattcct tatgctctgt tttgaaccgt tccgatagcc aattcagttt taaaccaata
     6361 cctccctgtc ctttcaagcg tggatagtga attttcattc gcaattctgc tgggaaagaa
     6421 agatcctgag caaattgaaa tagatcacaa tttctcatat tttcaggtgt ttccccaata
     6481 ggcaacaatg ctaaataact actccctaac tcatcttcta attgaagaag gcctttatgt
     6541 tccttcggta tacgtacata cgttccttga ttgtctttca gtacttccca attattttgt
     6601 tcttctactt tgtgagccat tcctcgaata tattgcagac gattgagata caacagctct
     6661 ttttttacta agggacgaat agaaaagcta taaagtaatt gtgtcacttc ttttacataa
     6721 ggatcacttt cagaaaacaa agtttccaaa tcatctactt ctaatccaaa taagcgtgct
     6781 aaacttcctg ctaaatctaa ccagattaga tttattacat catgaaaact ctccaccgtt
     6841 gctttttctt tgactttgat tcctaaaata aatgaaggac gatagacttg accttctcgt
     6901 tccaacaatg tcactgtttc ttttgcataa tacgcaccaa ttgcttgact atcgggagaa
     6961 aatccctttc ctaatgtgtc aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc
     7021 aactccacat cttcataatc tgctaaaagc aacagtaaac gagccatcat ttcttttatt
     7081 tcctgtgtct tttttggtgt gttttttgaa attgttcgtg gaaaaacatg atagtaggcc
     7141 cagatacaat cctctccatt taacaaacaa tttttcttga atgcttttac tggttctttt
     7201 acaagcacca atgaatttca cctcccaata aaaaaggaac aaccagttga ttgtcccttt
     7261 ttctaatcct ctaaataaac atacttattc acaaaaagta attcttccaa tgcattgttt
     7321 gataccttgt cccagttata atacacacaa tcaccattca tcatacgaat catataacaa
     7381 aaccaatccc ataaataaaa atgcactttt tttccatcag gttttagttt tgccagtgct
     7441 aacacacata aataggggag tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa
     7501 atgacccaac caataaatga acgtaaaaat aaatattcaa agattacgac caacaaaaaa
     7561 gttgctgcat tgactaatgc aactggaaat ggaagacgcc aattgcttgt aatctgttgt
     7621 acgcgataag gttccgcata aacaatcgaa taatcataag gttcccaatc cacttcacta
     7681 tcctcctaat ccataaataa tttttcaatg attttaccaa acgcatcaaa tacacgtgtt
     7741 ggatcattta agaagaaata aactgcggca cctgctgcga tagatattaa tgcatttcct
     7801 acttttgatt tcataaaatt tcgcaatccc agataaacaa ccaccattat taaaaaatag
     7861 ccaccttgcg ttaatataaa atctacaatt cccttcagtg tcatttccgt tcttctcctt
     7921 tcctatccaa ccaattatgt gttaattttt caacataata ctgatttgtt tttttcgtaa
     7981 tgacaatcgt catttgttct ttttgtaaaa gtgacgtatc accatctcga aagacgaccg
     8041 ttgcttttac aataaaaggt tttttcgttt ctttataacg caatactttc atctctactt
     8101 gatcaacttt agctgtttga ttgagtcctt caggttcttt catcatgtat gacatatctt
     8161 ttgcattgtt tttcgcatac ttatctaaga aatcttccac aaatccttca acatctttcg
     8221 cttccgacgc atcaaccgtt tcgtataatg tgtcatcata agtaatcatt cgttgttttt
     8281 ctgtattctt tggtagtgcc gtaaaataag gataagcaat aatacgacag cccttgtttg
     8341 aaatagaaaa gggaagattg agtacttgtg tgattgtcac acttgtttct ttcttactct
     8401 ctggattcat cgagcgaata tcataagtta ctttataact agcagttgga atggtttcaa
     8461 tttcttttaa tccaataaac gaagcagcag ttaattttcg aacacctgaa acacttcctg
     8521 gttcttgaat aaagttttgt gcataaaact ctttttttaa tgtttcttga cgttgttgtt
     8581 cttgttcatg attatcgaat gtattcatat acaaagcaac aaaacgattc ataaaggatt
     8641 caatttcagg tgtgtacgtt aattctgacc gactttgtac ttctttcttt tgtaattgtt
     8701 ggatttcatc tttcaaaaac gacacttgaa ccttcgtttt catggattga aaaaatgcca
     8761 agccccctga aaaacaaaga aataaaacca aacaccaaat ccatttcact gctttttttg
     8821 aatggatttt cttttgcttc ggtactttct ctgtataaac tttttctttc cgccgtagtt
     8881 gtcgtttttt cataagctac tccttctagt atgtttttac tagtacgtac gattgacaac
     8941 gataagcttg tttttctgaa aaatagtcca ttcaattacc ctcattttct attccttttc
     9001 tctttctttt atatccaaca aatccagcaa tacttgtcaa cactcctcct agaacagaaa
     9061 gtgcaacatg tatttcttca ccagtctttg gtaagacttt tgtttttcca tctgctgttt
     9121 tgatttcaat attgccactt ggaagtttct ttacaggaat cgacaaatcc tttaccaaat
     9181 tcccttcact atcttggatt aatggaatac catcttttac accaacaatt gtttgatcac
     9241 ctgctacttt taatggggtg gttggtgttg gaatatgggt caattcctct acattactag
     9301 tgacaatcgg tacactcatt ccttgagaag tattcgtcgt aatttcaccg cttggggtta
     9361 ccgatactgt gacttctttt gctggaacaa taggtttatt ttcttttgga attgcttctg
     9421 atttttcaac aggttgtttc ggttcttctt tttgttcttc actggtagac gacatcgttg
     9481 gctgttccgt acttgacgaa tcagtattct tttcagaaga actggttgtt cctgattgtt
     9541 ccggagttgt gactatactt gaatccgttg ttgttgaact atgcacacct tcttcttcga
     9601 tcgaatcagt cggtaaagtt gattcaggaa actctggttc tggtgtcact ggctcttttt
     9661 caggtttctc tgactgcaat tgttcaggtt ctgctggttt ttccgttgtt cctccattta
     9721 gctctgtctc aacatctggt aatccttccc ctggcaatgt ttctacaatt tctgacttag
     9781 gtgtttctac agtttcatct gcataaacca atgaactccc taattgaaac gaaaataatg
     9841 aaattcctag tgttgttacg actgctaatg ttgttttttt catgattatt cctccaattt
     9901 tttatgcaat tcgtgttttg ttcatagacg ccgcactaat attatctgaa cgataaaaga
     9961 actcccgtcc gttttcttct aacccaacat atggattatt tttgaataat tgaatcaata
    10021 gttcactggt gttgtatttt ggatagtttg caaaaaaata atactgtgct tgtcgcccat
    10081 ccgaaaattc aacaagtaat tcataacgat accgcttcaa tccttttcgt tgttttaaag
    10141 gatctctata ccaacttgtc catcgttcca ttcgtggata gagatagaca cgatacacat
    10201 agaacaatgc cataccacat accaccaaaa ataataatcg ccacataaaa attccccctt
    10261 ttcttctacc tttcttgaat catcgaacat tcataccctc tatagagatt tgaaaaatcc
    10321 atcatcaatt gttcatataa acgttgttgc aaatagcgat tgtttcgaat acataattct
    10381 tcatagccat caatcacttg atcttctttt tcatagcatc ttgtagctct atgacgctca
    10441 gcttgcatga cataaccaaa ctctgcacca tatcgttcta ctgttaataa ctgttgacta
    10501 cgcaataatg caatataaca atcacaaaaa tgttcaaccg acatttgatt tttctcacgt
    10561 ttgtgaaata ggtattcttt agaaagcaca ttattcgtta aacaatctga taaatagcga
    10621 taatctggcg gtcggttatt ttcatctgtg aatttttccc ataataaact gatttttatt
    10681 cgttcagcgg ctagatagtc ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt
    10741 ttatcacctg aataaacaaa tccatataat gcgaaacgaa ctcttccttt tcaattggct
    10801 ctcctaaaaa ttccagtgac ataccatctt ctccttccta aaaaaaataa aaaagcagaa
    10861 accgatttgg cttctacttt tctttccttt tatagctctt ttttacggaa gaatccgcaa
    10921 atgacatttt ttcaaaaaat caatcggcac aagttcacga aaacttggag caattttgga
    10981 tagaatcact tccccatctt gtaaaataca atcataaaaa tcatcagtta cttgatactt
    11041 acgcttctta caataattga taaaatagta gcgatcattt tctatttcca ttcgttgttc
    11101 tactaagcgc atacgaacat catcatagaa ataagccaca aatcgccctt caagttcatg
    11161 tttttcaatg ggatactctt ctggtaacaa tctatttttt cgtcgtttat acattgctgg
    11221 ataccttaga agaagttcat ctaaacgtct gtcagtatat ggtaactgac gaatcaacga
    11281 ttctaatcgt tctttataca gatagaataa ttggcgcaag caatagtgta ccgcctctgg
    11341 attttctaat actgttggat cacgtgacca atacgcacca acataaatca taattttttt
    11401 catttagaca atcacaccag caatactaat gaccgatcca caataggtag aacaagtggc
    11461 atagcgacca atttcctcta attctatctc aatctcttct aaagaaagat atgtctgttc
    11521 ttctgttggc tggcgactca tttcccaagt gacagtcacc aatgaaattt cttccataag
    11581 aggtttttcg ctgctaagtg ctgccacaca gttttcatat atcttcattt tatcgcagcc
    11641 ttttttaacc gctaaaatcg caggtattgg caatgaaaat cgataaaagc gaatgaattc
    11701 tagccttcct ttccttcggc taaattccaa ctcttcgagt tcttgttcgt atcccttacg
    11761 aacttctaat aagcgtgcaa tttcttgcca gttctctttt tcctgataac gtccagcttt
    11821 taccgcttgt tcaatataag gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc
    11881 atttaatgct tcgattgcag cctttaaatc caacaggcgc agccattctg ttgtcgtata
    11941 tgcattcatg caattttccc tcctgacgtt cccatccatt ctagatagtt ttgttgctcg
    12001 tcatttagtt cggcattatc aatcccttct tgtacaaagt ctcttcctaa aatacgatct
    12061 gcttcttgta acaactttaa tgtcggagct acttgtttca aaaaccagcg ttctgaacga
    12121 tctaatgaga atttttctgg ctttactgta aaatccaatt cttccatcga acgaaataat
    12181 tttgcccatc gtttatcaac aatccgtgca tcactttcca gtctatccca aacaataagt
    12241 tttgcattaa ttaaaccaac gccaatctct cctaaacgtt tcggtgaatc acctaaaata
    12301 aatgcttcta ctgcttgtgt tgctttctca ttggctaacc gtaactcata tcgattataa
    12361 ataccatatt tcgttaatgc ttctaattct gaaatttttt ctcggttcgc aatttcaaaa
    12421 cgtttctcat aaaaattaaa aaagagagga ctctttcgcg atccaaaata caaagataat
    12481 ccttcacttc gaaaatagcc atcatctaaa aaacaaccgc caatattctg gaatctctta
    12541 aagtccattt ctaataaacc tgctttttgt ttttctagta attcaaagag atcgaaatgt
    12601 tcttccgttt ccaatcccca aagttcatct aacgcaatat cgaatcggct acattgcaca
    12661 cgtgtataac cagcttccaa ctttccttga tttttccaaa tggaatatag ccatgttcgc
    12721 caatctgttt gttgttcacg catgacttcc tcatattccc ggcaaccttg tcctgaaaat
    12781 tgcacaagta cattttctgt tacagttcgt tttgaatgcc agaacaccca gatattccca
    12841 cgaacagaaa gacagtcaaa aagatacaag cccgttggac gtgtttcaaa ttctgtcaga
    12901 tccattcgta atacacgttg aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc
    12961 aaataatcaa tcattgctcg aagatctatt ccgcttttca tatacaatcg aaagcgttcc
    13021 ttgattcgtt gttcaaaatc ctctgggatt tttcttttcc cttgttccat ctttctaatg
    13081 taatcttttg agcagcccat caattgcgca aatcttgctt gtgatagcgg ctttttatct
    13141 ccacttatat tatgaattgc acttaacttc tttctgtagc tttttagctc aatcggtgtc
    13201 attttctcgc tccttttata aaataacaaa atcgattttt tttaaccctt tagaaacacc
    13261 gatatatcaa ctagtccccc tcgttttttg ggaaaagtag tacaactgct gaccttagat
    13321 aatcacttga tacaaaaggg tttataaaac ttttttctat gtgttatgtt atttctgccc
    13381 cccatattat atatcggggg gctttacaca aagaggccgt gcgcaagcgc acgacctctt
    13441 tgtgagctgc acacgctgct agcgagtgcg cattataggc ccatgcttcg cagccttaat
    13501 gcgcaccgcc aggcagagtg cggctaccag actacgtcta gagccctccc gatagataat
    13561 aactacaaaa ttaaatgatc taattttgtt atttcactat atttttcagg agaaaaaacg
    13621 cgataaattg acaaagttaa atcctcttct aaatctactc gataatgata caatcgagtc
    13681 aatcgttcta tatctttttt cgttagcaat acttgattaa taacttttcc tgtaaaaaca
    13741 cgttctttgc gcaaaaagcg gtaatcagtt acttgttcaa tacgattcaa ccaatcataa
    13801 aacgttctgg aagaaagaga aggattaatc attcgtattg ctgtagctac tctataataa
    13861 caactctctg ccatttaact tcacctcctt agttgattac aaaatatgcg catcagaaaa
    13921 acttgttcct tttggatctt ttgctaaata catttggcgc tctagttgtt cttgtaacaa
    13981 ctgattatca tctcccatcg tttgatcttg actatcctct tttactgtaa aatcatagac
    14041 aatcatttca gtaaatttct caaattcttt gataaagtga taatctttgt aaacaaatgg
    14101 cgcaaaaaac tctacaggta catcaaatcc tggaaaagca tatccacgac caggttgatc
    14161 tttgttgtaa aatggcttat ttttaaaatc ttctccaaaa gtcattaaat aaccaatggg
    14221 agacaatcga ccaacagaaa tacggaatgt taaattatcc cgaacaccac ttggaaaata
    14281 ttctgcatct ggtcgttgca ttgctaaaat aatgtacatc cctgcttgac gaccctttaa
    14341 tactaatggc tgaatcaatt tatcgactct atttctcgct tccatatcca acgaaccata
    14401 aaaagctgcc cattcatcaa aaaagataaa atgcggtggt aacccataat aagcataatt
    14461 tttccctggt tcatatttag gatctttcat ggttttcata taggcatacc gttgattcat
    14521 ttcttttaat gcatttcgta aacaaataat catttcttca tttattttgt aaaacacatg
    14581 tcctttgaat acatcgacgt cacctaatgc tgctaaatct gccgcttttc catcgcaaat
    14641 atctactgtc ccaacactta aacaagcttg aattaaagaa tacaataaat aggttttccc
    14701 accaccgatt gcaccagcaa tcaacatatt tggtgattga tcatataccc aatccaaagt
    14761 tttcattaaa tgaatacgtc cattttctgc tatacattga tcaataggaa tccgaccaga
    14821 atctgaaagg aatacataat tgattcgggc tttttctcgt tcttcacgaa ttaaatcacg
    14881 caaaaatact tcagataaca aatgacccat ttttaaaaat cgttcgtgaa aacgcccacc
    14941 atctaaagga aaagaaacca ctaattcttg tttcttttga taaaaataaa cttttggtaa
    15001 tctcattttt tcccttgttt gtttctcact aacttgtttc tttgtataaa caaaccgatt
    15061 gtcaattaac attcgaacaa gtaactgacg ccaataaaca gaggcaaaat atccttcttt
    15121 tagtcgcgac caatgcatca accctaaaat taacccacta accagcaaca aatacaacac
    15181 aatacttatt ccaggaataa gatacatttg ccaattcgaa aacgtactta aacgtggata
    15241 aaatactttc caactaaacc aacctaacag aatcaaaaat ggaaacaaaa agaaaccata
    15301 atagcaaaac cataaataac gattgaaata gcgaattcgc cgaccacgat acttgaaaaa
    15361 atgagctttc aattgtcaca ttcctccttt ctgtttttac ttttcctgtg ctaaaaactt
    15421 ttgacaaaaa gcaactaata caggaatatc acatttagta aaaaaacata atcgaaaaag
    15481 atttaatcga tttttataag ggtgtgcaca catattacat attcgtacta tacgaatcca
    15541 aatcacttcc tgatgatctt gtttccactg gtacggtaat gaaaaacgaa caagatcacg
    15601 taaaaacata cactgcccaa taaccgcaca aatatatgca tttgtttgta caaaaatcgt
    15661 tggattgtct ccaaaatact caatcaattg ttggatattt tgataacaag cacaatctct
    15721 caaagtattt gattggtttt atctgaattc aaaatctccc cttccttcta tcttagataa
    15781 aaagaatagg aagactcctt cctattcttt caaaaaaacc ttattttttc tctttttcct
    15841 tagaatcaac acttggttgt ttttgatcta aacctttttc ttgtgattgt ttcatattag
    15901 gattatttgt tggagcttct actttctcta atcctttcgc ataaatcact aaatctgaat
    15961 attcacgatt gccatcttgt gctggaatat ataccacatg atattctgga tcaattaatt
    16021 tcacaggatc acgtcgttta aaaccagtca aatctacatc tgctggaaat ttaactcgtc
    16081 gtgcaatctt tggttcatct ggatttccaa aaactaattg tgctcgctgt gatcgaattt
    16141 ctcccgtata atcgcctgtt tcattatcaa atacacgatc agactcttct aatgcgccaa
    16201 agcgtacggc tcctaaacaa gtttcatcta atgcttctgt aatgtcataa ttatttggaa
    16261 attttaatcc catcttacaa aacctcccca atttttacac cagttaaaaa gacaccaaca
    16321 tcttgatagc cagttgttcg acgctctgtt tgtcccatat catttgtttc acgactattc
    16381 actcgttctt tacgaaaaac aaaataagct tcttcaaatg aaatacgcac ctctttttct
    16441 ggtaatcgta atcttttatc ctcttcagct gtgagataaa caaaaaaagc aggctgttct
    16501 tctcgcgaac actctgcttg taattgaaca tatcgcccag acacaccatt aatttccttt
    16561 ggtggattag gttcccgtga acccaataaa aatacttgac ctaaaaactc tttcttcata
    16621 cgcaatgctt tttctgccat aaagtttttc ctcctcttaa aaaccaaaat aaattctaaa
    16681 tatttctaca ttacaatcaa acaagcggtt aaaaatccta accgcttgtt cctcatgact
    16741 aatctttggc tttacgtact ttataaagtg ttatacctaa agcaatgact aatactacta
    16801 atccaagaac cataagtata ctacttgttt gttcacctgt ttttggcaac attggtacaa
    16861 cagtagctgg tttaacccgt tgttctggtt tcactggttg cggtggtttt ggcgcttcca
    16921 caatttgaac cgtttgatct ttgtttgtta gatctacatt gtgtttcgcc acttcttcaa
    16981 acgtttcttt ctcatcttcc gttttcgctt ggagccattc cgcaaaactc aagccttttc
    17041 ctgcaagatt gcgaccgtcc actaagaatt caaataagaa ttccatcatt tctttatcgg
    17101 ccgcaaagac tttttccgca tgagtgcctt caaatggtgt gccgtctggt gttaagtagc
    17161 cttgaacaat taagcgatag atttgaccaa tcgcaagacc gtctactttg acccattcaa
    17221 gcacagtttg atttggcaag gccaagatct cttttccgcc atttttattc gtcgctttcg
    17281 tcgtaatggt tggattactg attttgatgg tttggtcatt gtcggtaata tctgcgtgag
    17341 aagccaataa tacgccttct ctaaacagat cttcaaagac cacgacttct ttgcctcgca
    17401 acgcacgtgc gtctagaata aagtctagat tgactgtacc atccgttgtt tctgccacaa
    17461 acgttaaggt acttgtgact tcttttccat ctactagcac tggtttttcc gttactttgt
    17521 ccattagaac accgttgact gtgtatgttt ttccaacgac caaatcgtgg tatttcacag
    17581 cgtctgtaat ggtgactttc gttaatggat taacctcttt caagccagtg ggtttatgtg
    17641 tagccgttgt acgtacactt gggttagtta ctcgtaccgt ttgccctttg tcgttgatgt
    17701 ctttatggtt tgctagttct tcatcatttc taaaaagact ttcaaacacg acgatttcac
    17761 gaccatttat acctgtcaaa tcaaaggtaa atggtacgat caccgtttca ttttctgctt
    17821 tggcaataaa gccaacactc gtttctaccg tatttccatg cacaagcaag ggttcaccgg
    17881 ttgctttatc cattaagatc cctttgacca agtatgcttt tcctggaaga atctcggtaa
    17941 tttcaacggt atcgtttaag gtaaacaatc ctgttgggtc tgcgactgtc aatccgtctt
    18001 caaagacagc cgtggtatga acggttggat tattcacttt cacactttgg gcttcatcgt
    18061 tgatatccgc atgtgctgca acttcaatgc catcacggaa aagtttttca aaaacaacga
    18121 tcgtttgacc tttcagaaca ctagcattaa ccacaaattc aaggtctttt gtgccattga
    18181 ctttgtctgc aataaaggcc aaggtacttg tgatttcttt gccatcttgt aaaattggcg
    18241 cattcgttgc cttgttcatc aatactcctg aaatggtgta ttcttttccg acaaacaagt
    18301 tggtataaga aacactgtct ttaatgacga tcgtttctaa tggatcaacc actttttcac
    18361 catcggcttt atttgacgct tgtgtttcta gttttggtgg aatcaagtaa ttacgtacag
    18421 aaaccactaa cgtttcccca tcatctggcg cattttgttt gaatgcaacc gtgaatgctt
    18481 gtttatctgt atttggttgg aaactttcta atggggcatt ttccaagaag taatagtcat
    18541 cggcaactag acctttcact aaaatttccc cgttttgatc agtggtatat tcaccaattg
    18601 cttgattttt accgttatac aaggtaaagt tcacacctgc gagcggcgtt ttcttcttgt
    18661 tccattcttc atacttgaat aattttacac ttttccgtgc ttgacggttc gcaaaatgga
    18721 tcactttgat cggttctgta tcattggtaa tcgagaacac taatgggttt tgattcaaaa
    18781 cgtaatttac gggggctttg atttcttcca aacgatagcg atcttttcca taagtaagtg
    18841 taccgttagt taggaaaaag cctttttcat tggttttaaa gatatccgtt gtttctgtat
    18901 catttggaat ctccattgtg acataacgat tggctaaagt atcaaaaatc ttgaattctg
    18961 tattagcaag tggaatcact tttcctgttt cttgatccac tttcaccact ttaagacgtt
    19021 gttcaattac tttatcttct aaaacataat ggaaagtttg cccattttca gaaattgtga
    19081 cttcaagtgg ttcaaatagt ttataccctt cttttccttt ggtttcagaa agaatgtacg
    19141 tatcaaagac aacggtattt ttaccaaaag tagcataacc atcattgtct gtcacaacgg
    19201 ttttcaccac tttgccagtt gtttttgacg tcaaagtgat ttcaatgcca gctaattttg
    19261 gtttgttatc tggattcgtt gaatcgacta aatctttatt agcaactttc actaaatcaa
    19321 tggttccttt gatcacttga tcttttgcgg tcgcattgac agtttttcca ggttcaacaa
    19381 caatttcaat tgcttcatca gaaagcacat aaccttttgg cgctttgatt tcttttaaaa
    19441 catatttgcc aggatcaaga tctttaattt ctgcctgtac tttgccgtta atttttttca
    19501 aggtaatttc tttaatcttt ttcccatctg cttgatacaa accatagacc gcattttcta
    19561 aagaagctgc accttgtgct ttcacaccag tttccgcatc ttctttaaac acacgaatgt
    19621 ttccttgttt tagaatttgt aaattaataa cagacgcatt aggttctgtt ggatgtaaca
    19681 caccaattgt ttgagctcct ggacgacgat aggctaatgg cgcaccttga actttaattt
    19741 tattcaattg aatttttgct ttatccggtg cctcttttgt gactgtaatc catacttcat
    19801 tgccgtgaat agatacattg acaccttttg gtttttgaat gtctttcaca tatttcaaaa
    19861 aatcattttt attctttact ttcaacgttt cgccaatttt taccgtatgt gtttgattat
    19921 taaaagttgc tttttcacca aagttattaa tcaaacgttg aatttctgct tttcgacgat
    19981 cataatcaac acggatattt ttgattggga tttttgtgcc caaagcttcc caaatcataa
    20041 attgtgccgc aatgtattgt tcatcggatt tgtctttatt tgtgacatat cctaaataag
    20101 agatcaagct tgtttttttc cgtacatttg aatttttcaa atagtttttc aaagcagtag
    20161 ctgtataaac ttgaccttct gcggctgaga caccaggctc aatacaaaac gccacttgtc
    20221 cattaatctc aaaacgaacc atataattta aagtgaagtt tagattgttc ggactagaag
    20281 taacaccaat attttcgccc caatcaattt tccgaccacc taaaacagct gcattggttg
    20341 cacggcgaac acgaaccgaa cgattggttg gtactgttac taacagtgta ggtgtacctg
    20401 tttttggaat gttctgctct tttttctttt gattttcaat actagccgta gattctttgt
    20461 ttggtttcgt tggttgattt tgactagctg cggttgaaga gattgttttt tcaacgtttt
    20521 cctcattttt tccttctttg tcagcagtag ccgctggttg ttccactgtt tcttctattc
    20581 gtttcttttc ttccgcttgt ttctcagaag actctggctg cacagaaggc gttgcttcgg
    20641 cagctttttc tccttctaga gtggacgttt ctgaagcagg ggttgtaatt gaggcagttt
    20701 cttcaactgg ttgtgaagta ccaggactat tccctgggtg ttgtgtttcc ccaatcgtct
    20761 gatcgagagt cgttttggaa ttgaccgttg gcgcttctgt cacaggggcc tgttcaacag
    20821 tggcttcacc agctgggacc ccggcttctg ctggttggac ttctgtcact tcagaagcga
    20881 cttgggcaat ggcttccatc gtaggttgta tagccgtcgt ttcttctgca tgtgcttgag
    20941 cagttcccac caagaaacag aattgagaaa aagccaccaa tgcgagtgct ttcaaccaat
    21001 tcattttcgt ttttgcatac acaagaattc ctccttttcc tgagaatttt atataataaa
    21061 acaaaacgcc gttaccaaaa gtgataacga cgtgaactgt ctcttctatt tttatctggc
    21121 gctcttatct gaacacattt catcaatgat tttaccctcc cactataatt acagaacaaa
    21181 aaaagaacct gcttcgtgga aatcaactgt ccatttccca attggaatac cagctttttt
    21241 ggcaacttca ccatcaccaa atctcatgcc ataccaatta ctaccttcat ccttaatttc
    21301 tttccaacca tatgacatag cttcatctaa tgaatcaaaa atcatgacac tattgccaat
    21361 tgttacagga tctggcttaa gatcttctgg aagagaaata tcaatcatac cataatgatc
    21421 cgattcctca gtattattgt cattactagc tactggtttg gttgttctac tggcttattt
    21481 tgatacaaca agtactctta ttcttctttt acttctccgt tttcaatttc ccattcaaac
    21541 cactccgcaa agtcttgctt aaaaccatct actcgatacc tattttgtaa ataggcatct
    21601 tctgacgtcg taggatctaa ataggtaggt ttattctctg gacgagcata gacacctacc
    21661 catgtttcgt aatctccttc aaaataacta tctaccaaat agcctttttt tctaagaatc
    21721 tcccgaactt gatttttaat tattgatact tgttcaaaac catcactcat tctatcacct
    21781 caaataactc tagattctca taaatattcc cgacaatttc aatcctatca atgatctcta
    21841 acaaatcgct acttatgctt ccccattcgt ataagaattt cccttccgca aaataaattt
    21901 tccataaact tcttcatgat cgtcccaacc aatatcactc tcgaaaaatt ccacgttgtt
    21961 cttatctttt agctcagtaa atgccataag ttcaacatgt tttccaacct cgaaacaatc
    22021 acaccaccca tcaaccgtta cacgtgtgcc ttcaatacct aagaaacttt gtcctcgtgt
    22081 attaaacact aaaactttcc gcattttttg ctcattaatt aatcacgcgc gaaatctagg
    22141 aatcacaact tattcgcttc ttctttataa atcgccgatt gtaaaattaa gctagacaac
    22201 taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta taaggagtga
    22261 gtacaaatga cctataacca tcttacacca actgaactcg taatgataga agcttatttc
    22321 aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg acaaactatt
    22381 tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta taaacaatat
    22441 aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga gcaacaggaa
    22501 tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg tcgagcagag
    22561 tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa aacctttgac
    22621 actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga
    22681 aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca gtttgatcat
    22741 gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag cgcggtcatc
    22801 acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg cagacaggct
    22861 aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa
    22921 tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag taataccaac
    22981 gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt gaatgaacat
    23041 tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa tggtgtatct
    23101 gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa atcattacac
    23161 tatcggacac caatggaagt atttttgagt tacgtcacaa aagatgattt gtcta
//
LOCUS       DAZKOK010000036        22584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000036 DAZKOK010000000
VERSION     DAZKOK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.036"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..640)
                     /locus_tag="ACG90W_001227"
     CDS             complement(188..640)
                     /locus_tag="ACG90W_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0477602.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(653..1063)
                     /locus_tag="ACG90W_001228"
     CDS             complement(653..1063)
                     /locus_tag="ACG90W_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0477603.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1338..1550
                     /locus_tag="ACG90W_001229"
     CDS             1338..1550
                     /locus_tag="ACG90W_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0477604.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1553..1765
                     /locus_tag="ACG90W_001230"
     CDS             1553..1765
                     /locus_tag="ACG90W_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477605.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1905..2060
                     /locus_tag="ACG90W_001231"
     CDS             1905..2060
                     /locus_tag="ACG90W_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477606.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2057..2281)
                     /locus_tag="ACG90W_001232"
     CDS             complement(2057..2281)
                     /locus_tag="ACG90W_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0477607.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2359..2547
                     /locus_tag="ACG90W_001233"
     CDS             2359..2547
                     /locus_tag="ACG90W_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477608.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2544..2717
                     /locus_tag="ACG90W_001234"
     CDS             2544..2717
                     /locus_tag="ACG90W_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477609.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2686..2814
                     /locus_tag="ACG90W_001235"
     CDS             2686..2814
                     /locus_tag="ACG90W_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477610.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2873..3208
                     /locus_tag="ACG90W_001236"
     CDS             2873..3208
                     /locus_tag="ACG90W_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477611.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3441..4382
                     /locus_tag="ACG90W_001237"
     CDS             3441..4382
                     /locus_tag="ACG90W_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0477612.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4384..5274
                     /locus_tag="ACG90W_001238"
     CDS             4384..5274
                     /locus_tag="ACG90W_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0477613.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5317..6117
                     /locus_tag="ACG90W_001239"
     CDS             5317..6117
                     /locus_tag="ACG90W_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0477614.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6132..6983
                     /locus_tag="ACG90W_001240"
     CDS             6132..6983
                     /locus_tag="ACG90W_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0477615.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7049..7183
                     /locus_tag="ACG90W_001241"
     CDS             7049..7183
                     /locus_tag="ACG90W_001241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477616.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7184..7645
                     /locus_tag="ACG90W_001242"
     CDS             7184..7645
                     /locus_tag="ACG90W_001242"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0477617.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7671..8123
                     /locus_tag="ACG90W_001243"
     CDS             7671..8123
                     /locus_tag="ACG90W_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477618.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8229..8423
                     /locus_tag="ACG90W_001244"
     CDS             8229..8423
                     /locus_tag="ACG90W_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477619.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8454..8747
                     /locus_tag="ACG90W_001245"
     CDS             8454..8747
                     /locus_tag="ACG90W_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477620.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8678..9217
                     /locus_tag="ACG90W_001246"
     CDS             8678..9217
                     /locus_tag="ACG90W_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0477621.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9292..9759
                     /locus_tag="ACG90W_001247"
     CDS             9292..9759
                     /locus_tag="ACG90W_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0477622.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10506..10736
                     /locus_tag="ACG90W_001248"
     CDS             10506..10736
                     /locus_tag="ACG90W_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477623.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10895..11728
                     /gene="terS"
                     /locus_tag="ACG90W_001249"
     CDS             10895..11728
                     /gene="terS"
                     /locus_tag="ACG90W_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0477624.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11721..13136
                     /locus_tag="ACG90W_001250"
     CDS             11721..13136
                     /locus_tag="ACG90W_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0477625.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13148..14677
                     /locus_tag="ACG90W_001251"
     CDS             13148..14677
                     /locus_tag="ACG90W_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0477626.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14595..15647
                     /locus_tag="ACG90W_001252"
     CDS             14595..15647
                     /locus_tag="ACG90W_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0477627.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15649..15966
                     /locus_tag="ACG90W_001253"
     CDS             15649..15966
                     /locus_tag="ACG90W_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477628.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16093..16716
                     /locus_tag="ACG90W_001254"
     CDS             16093..16716
                     /locus_tag="ACG90W_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0477629.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16731..17621
                     /locus_tag="ACG90W_001255"
     CDS             16731..17621
                     /locus_tag="ACG90W_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0477630.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17644..17859
                     /locus_tag="ACG90W_001256"
     CDS             17644..17859
                     /locus_tag="ACG90W_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477631.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17871..18212
                     /locus_tag="ACG90W_001257"
     CDS             17871..18212
                     /locus_tag="ACG90W_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477632.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18212..18583
                     /locus_tag="ACG90W_001258"
     CDS             18212..18583
                     /locus_tag="ACG90W_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477633.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18576..18971
                     /locus_tag="ACG90W_001259"
     CDS             18576..18971
                     /locus_tag="ACG90W_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0477634.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18973..19350
                     /locus_tag="ACG90W_001260"
     CDS             18973..19350
                     /locus_tag="ACG90W_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0477635.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19351..19959
                     /locus_tag="ACG90W_001261"
     CDS             19351..19959
                     /locus_tag="ACG90W_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0477636.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19959..20300
                     /locus_tag="ACG90W_001262"
     CDS             19959..20300
                     /locus_tag="ACG90W_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477637.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20542..>22584
                     /locus_tag="ACG90W_001263"
     CDS             20542..>22584
                     /locus_tag="ACG90W_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0477638.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWI"
BASE COUNT         8105 a         3310 c         4632 g         6537 t
ORIGIN      
        1 tttgttctaa ttcataagct tcttttttta ttgtgttagc tttcgacagt ttagtgattc
       61 ctataattaa taaaataagt ccaatcaata ataacattca aaactcctca tttctttgat
      121 ataattattg tagagtttac tcagaaatga ggaaagagtc catgtgaaag catgggcttt
      181 tttattttta atgttgtaat actaaatgtt caagattatg aggaagccct aaatattcaa
      241 taatttggaa ctgactcata tcagataaat cttgttcatc tattgttcta gttaacattt
      301 caattgcaaa catattagct tcagcttcta taccataaat catacaacta aggttgtttg
      361 ctctaaaagc tgctgtgcaa atacctttat ggagtctaac gtgggctatt tcatgccata
      421 atacaaatct tttcatagaa ggtgataggt tttgattaat taatatcaca taacatcttt
      481 tattgtgtat cgtttgtcct aatgttttat tccctaaatc agtttcaatt aaatctatat
      541 cagaatattt gattatttta tatggattaa tcgcatcaga tccaaactct acaaataatt
      601 tttctatttc ttctaatatc cagtatttgt ttaaatgcat accaaagccc acctattgac
      661 gatatttttt aggagtgaat tttttctttg ctttaacttt ggttaaggca agtgcaagtt
      721 gaatggcatc gtataatttt tccttatctt catcgtctaa aggttcacca taataatttg
      781 tttcagcatc tgaattcaag ccttcaagta atttttcagc ttgtaaagca atatcttgtt
      841 tatctttttc tgttaaatca taataatggc gtttatcagt acgtcctaaa agataatcag
      901 ttgatacatt aaagtaatct gcaacttttt gtattttatc tccagatgga gtacttttat
      961 tccatctaga catgcttcca tttccgaaat ttaactttct ttcaagttct gccaacgtca
     1021 ttgaacgttc ttttaataag atttttatac ggtctaatag cataggatat gcattccttt
     1081 caaaataccg acaaaaaaag tatttttctg caaattatct atttacaaat agagaatttg
     1141 cagttatact agttttgtaa gcaagatatt tacggcatac aaagactaaa agcaataaac
     1201 taatagtctc cccagacatc agtaagctcg ctttttgtgc gctgtatttt gtatgcttac
     1261 ataatagcat atttgcagtt tttgtcaaac ttattagaca aaaatattag aaaatattcg
     1321 ataagggggt atgaagcatg actttaaaac agcaagtatt aattgcttta gtaaagaaag
     1381 gttggagtca aagagagtta gctcgacgaa tgggtatatc aattacttat ttacgagata
     1441 tcttcatcga aaaaagaaaa cctaaggaac gtttaaaaca aatcgaagaa cttttggata
     1501 tcaagttaga agtagattct aatgatcagc catcagaaca ggaggcgtaa aaatggtaca
     1561 acgtttgagc aaagcggagt ttaaagagaa atataagtat tctgaaagta cttatcaaag
     1621 aagaatgaag gaatttaaaa aatcacgttt tagtgagggg tataacgcag ttacttccaa
     1681 agaaataaca attgatgtcg atttatatga acagtttaga acatggcgtt ctaaaaatcg
     1741 atttagagtt agagctgttt gttaatagct aaaaaatcta aattcaccac ttctgaaaag
     1801 aggagttgct gctctccttt caagtttgcc aaagtccacg ttttttcatt ttggcaactc
     1861 ctcttcttgg aggtggtgac ggcgagagac gaaggaggga tgacatgaag gaagaatcaa
     1921 aaatagggat tttgaatgag aacaattaca caagattaat tattgaaaca gaagaaggga
     1981 aaaaagtagc agaagttaca ttgaccgatg caactcctgc tgacggttat cgtattcgat
     2041 taactcctaa atatgattaa ccttttggtg ggtgtggatc gtttccgtga ctatcacggc
     2101 tttgaatttt tccatctctg ccatgaataa atagctcaga tccttgattt ttagaaatct
     2161 ctctagcgat ttttactgct tctgattttg tattagtatg tgcagtcgct ttagaatttc
     2221 cagcaccttt gacattccaa ccaccattgg aattaggtac aacatgttga ttttttccca
     2281 tagttttcac ctccatttct atagaaatat tttatcaaat agaaatggag aaattcaata
     2341 aacgaaagga gaagtgatat ggcatatacc ttgcaacaag aacatcaaat tctcggtttg
     2401 attaaacaac gcaggaaaca attacaagat gaccgtgcag cgcttagaaa agccgatgag
     2461 ctatcagata ggcaagctga actaattgct tctgaacttg aggatttgag aatgctagaa
     2521 ataaaaaata gggagattag attatgaaga agacagacac actttttata ggattcattt
     2581 tggggttatt agtgattgta gcgcaccaaa gtattatcgg gggaagcttg ttcgcagcat
     2641 tgatggtttt aatcaatctg cttgattcaa aagaaaggag caactatggc acgagaagaa
     2701 gcgctaaaaa tcggtaaagt gattgctgat aatcggtggg gaaatagccg ccctattatt
     2761 ttaagcaagc aacatatcga aaagcaaaaa gcatggcaac aaataaaaaa gtgactctgc
     2821 cagccagcaa agagtcacat acaaatttat aaaactagga gaattatatc acatgaataa
     2881 gaaaattgaa aatcttatcg aagaattgaa acgtgaatgc caaaagcaag gtgtttctat
     2941 tatttgtaca gcccaaaaag aaggggaact taaaagtctc gtttatggtg agacaacaga
     3001 aattttactt tgtcttgcta tgcaagaaga acatttagat gaaaatctcc cattgtcagc
     3061 acatattatg cgtagaattg ctgtggatgc ttacgaacaa gcgaaaaatg aagaagaaaa
     3121 tcaaccttca aatcatactt ttgttattaa taacaaggaa gatttagctg atgtgatgac
     3181 tcgtattctc aagggggaat ttcaatgatg aatgaaaaaa caattgatct actcattatt
     3241 ttatttttca cgttagtgat tgtagcgaaa agttatgtaa tctttggctt gttattcgca
     3301 attaccatgc ttgtgctttg cgtgttagca agcagaaaag aggttaaaca tgaacgagtt
     3361 agcaaatctt gacaactatt taactgatcc tgattatgct aaaccaccat atgaagcacc
     3421 aattgatgag gaggatgaag atgagtaaat ctaccttaga aatgagccat caagaatggc
     3481 ttgaagaccg taagaaaggc atcggaggtt cggatgttgg aacaatttta ggattgaata
     3541 aatggaaatc tccttatcaa ctatggctag aaaaaacagg acaagtagta cttgaagaat
     3601 caggaagtga gcccgcttat tggggtaatg ttttagaaga agtggttgct aaagagtttc
     3661 aggaacgtac aggcaaaaaa gtacgcagaa gaaaccaagt atttgaacat ccattacatc
     3721 catttttaag agcaaatatt gatcgggaag tagtgggaga aaatgccatt ctggaatgca
     3781 aaacagccaa tcaatttctc ggtaaagaat gggaaggcga agaggtaccg cttagctatc
     3841 tctgtcaagt tcaacattac atgaacgttc taaacaaaga ctattgttac atcgctgtct
     3901 taatcggtgg tcaaaaattt atctggaagc ggattgaacg agatcaagag ctgatcgata
     3961 caatcactga acaattagta gagttttggg aaacaaacgt tcttggaggt atcgagccaa
     4021 ttattgatgg tagtcaagcg acagctgatt tcttgaaaga gaagtatgca gatgttgaag
     4081 acgttcaaac gacgttacca attcatttcg atgaactagt tgaacagaaa aacgaactca
     4141 aacggactaa gaaagaaatt gaatcagcta tccgtcaagt agacaacgag attatcagcg
     4201 agctaggtaa acgtgaagcc agtatcggta tcactcaaaa aaacatcatc agttggaaat
     4261 ttgtccgtac gagacgtatg aactcgaaga aactagcaga gaaatatcca gatgtcgcaa
     4321 atgatgaaga gatttataac gttactgaat caagaaggct aaccgaaagg gagatcaaat
     4381 aatatggcaa caaatgaatc gttaaaaaat caattggcag caaagccaca gaaacaagtt
     4441 gcaccaggtc aattaggact taaagctttg atgaatacac caacaatgag aaagaagttc
     4501 gaagaggtgc ttcatgacaa tgctaatgct tttatgtcga atgttatgac tcttgtatct
     4561 aatgacagtt atcttgcaga tagtgaaccg atgtctatca tgagtggtgc gttaactgct
     4621 gcaacattaa atcttgggct agataagaat ttaggttatg catatttagt tccattcaat
     4681 agtaaaaaca agcaaacagg aaaatgggaa aagaaagctc aattcatgct tggctataaa
     4741 ggatatatcc aattagccca acgatcaggt aaatacaaag cattaaatgt gatcgaagtt
     4801 tacgaaggag aactaaaaag ctggaaccga ctgacagaag agtttgagtt tgatccaaat
     4861 ggtagaacgt ctgatgaagt cattggatat gttggctatt tcgagttact gaatggattc
     4921 aaaaaaactg tctattggac caaacaagaa atcgaagctc atcggattgc taacaataaa
     4981 gatcgcgata agacaaagtt aagtggtgtg tgggcatctg attacaatgc aatggcacga
     5041 aaaactgttt tgagaaatct tctttctaaa tggggaatct tgtccatcga aatgcaagaa
     5101 gccaccacat cggatgagag agtccaaagg gttcaagaag acggcagcat tattgctgaa
     5161 acagaagttg aagaagatat tcctgaaaga aaagaagcag aggttatatc tgaagaaaat
     5221 gaagatgtac aaactggatt atttgatgca tctaatccgc cgttaaacaa ataatgaggg
     5281 agctttctcc cttgcttttc tagaggagaa atacgaatga acacaggata tgtcaaatta
     5341 catcgaaaag tgatgaattc attcgtttgg accaatcctt atatgtataa attatggaac
     5401 ttgtgtttga tgaaagcgag tcatgagaat cgcaagatac tttttaatgg aaaagagata
     5461 caagtgaaca gcggagaatt cgtcacaggg cgcgatgcta tcacatctga gatgaacgaa
     5521 ggcgtgaaac gtgaacatca agtgaacagc ggttctgtat ggagatggct aaaacaattt
     5581 gaaaaaaacg gaatgttgaa catcaaatca actacgaaat acagcgttat atcaataaaa
     5641 aactggtctt tgtaccaaag cagtgaacaa caaatgaaca tcaaacgcac aacaagtgaa
     5701 caacaagtgc acacaaacaa gaatgaaaag aattataaga atgaaaagaa taataaaaca
     5761 tcatcgtcgc aaccacgcaa aaagcgtgtt tacgacaccg actcagttta ctacattctc
     5821 gcggaggagt tattcaaaca gatttgtcag aatcaggaaa tcaaaaagcc gaatctgcaa
     5881 agttgggcag atgacattcg gaaaatgatc gagatcgaca agcggaccga aaaccaggta
     5941 cggggaatga ttgaatggag tcagcacaat gtgttttggg catcgaatat tttatctgct
     6001 aaaaaattgc gagaaaaata cgacacaatg gcagcgcaag caaatcgtga ttataaaaca
     6061 aaacaaacta aaacgcttga atacgagaaa tttagcacag atgagttgcc tatttgagag
     6121 gaggcgtagg catggaaact gttggggaaa tcatggaaaa gctgatacag aaagtgcttg
     6181 tccaacgtgg cgaatgtcct gaatgcggac agcctttgta tggatggcgt acgaagaacc
     6241 ctgatggctc agaacgttgt aagccaacct gtatgcaatg tgggtataaa gcattgcgag
     6301 ttcaggaaga cttacaaaca gaacgaattt acaacgagag cctgaaagca agagcaatca
     6361 attttttcaa aggtggttct gttgtgccta atcaagcgtt gtttgattgc acattgcaga
     6421 attatcaaat tgtcgatcaa gaaacaagac aagcggttga agtaaccaaa cgctttgtta
     6481 attcagtctt gttaggaaat ccaagtcacc ttgttttaac tggaaaacaa ggaacaggta
     6541 aaagtcacct agcgatggca gcggcttggg aagtcttgaa gcgatcaaac tacgataaaa
     6601 aaatcttatt tatcgggtta caggaaatgc tggatcaaat caaattttcg tacaacaatc
     6661 ctgaactcag aaaaacgatt gagggatcgt tgattgcaga tatcaaaaca gcggatttag
     6721 tcatcattga tgatatcggt tcagaactag gaaaagatgc atcagatagt cgagcgtttg
     6781 gcataaacac gctaaattcg ttcttggatg cacgacagaa cctagcaacg attatcacaa
     6841 cgaacttgct tggtgaagaa ctgaaaaaag cttacggtac gagaacaata tcaagaatgt
     6901 ttgtcaactc tgatggattt acgatggtat tttctcaaac agcagacaag cgcataaaac
     6961 cagtcaaagg agcgatcgca tgaatttgac taaacaagaa aaaagaatct gtcgtcagat
     7021 aatgttgcaa attataggtg atcgtgacat ggacgaatta tctcaacaag agagtcgaat
     7081 ggttcggaag ataagtaagc taattattta tcgggataca gaaagatttc tcactgtttt
     7141 aggtaacgtc tacgagaatc cagagttatt ggaggtagag taattgccaa aaatattaga
     7201 tgcatgttgc ggtagtagat tgttctggtt tgataagaac aattctgacg taacatttat
     7261 ggattgtcgt cagcagtatg aagaattatc aacaggtcat gtgatcaatg ttgatccaga
     7321 tgtggtcgca gactttcgcg atatgccttt tgaagatgat gtgttcgata tggttgtatt
     7381 tgacccgcca cacttaagac atgtcggcga aaactcatgg cttgccaaaa aatatggaaa
     7441 attagacgaa ctttggccag aagacatcag gcaaggtttt gcagagtgta tgcgagtttt
     7501 acgaccttca ggaacattaa tttttaagtg gaacgaggaa cagataccac tttctgatgt
     7561 gttagaggct attggagaac agccattgtt tggtaataaa cgaagcaaaa cacattggct
     7621 tgtgtttatg aaaaaattca gctaacggag gaaacaaagg aggaaaagta atgaagattg
     7681 tattaaataa atgttttggt ggctttgggt tgagccatga agcaaaaatg gaaattttta
     7741 aaagaaaaaa tattgaagta ttcccataca tcaataactt tagctatgac tcagatgatg
     7801 aatacacgag atacacaggc caaaagctag gttcgatgga ttttatctac tatttcaaaa
     7861 aagacccgaa aatcgacaaa gttacaggaa tttatagcga aattgaccga ctgtacggaa
     7921 ttgctgatga ttccagtttt tcaagtgatt caaacagagg tgataaggac ctcgttgctg
     7981 ttgtcgaaaa actaggaagt gaggccagtg gaccatatgc aagtcttaaa gtcgttgata
     8041 tacccgatgg ggctgagtgg gaaataagtg attatgacgg cgttgaaact gcacattatg
     8101 gttttcaaac aggtagtatt tagtccacta atgacaacta aagaggaggc agaaggatga
     8161 gtgaagatga atactttgaa cagtagtcta ttaacgacag agatttttaa ctggaaaggg
     8221 gtgtagtcat ggacgcatcg gaagtagtaa ataaaattaa tagtctcaaa gaggttttcg
     8281 gagacgtcga agtagtgata aacgcacaag gctatgacaa cgaatttttt aagcagatta
     8341 ccgacattag cattcagcaa ggaatggaag acgaagacgg aaactttatc gatgaagtag
     8401 ccattttagt gacttgcgag taagtaattg acagctaaag aggaggaagc ggaatgaaac
     8461 taaaagacgg attttactcc agcggccacg gcatcggcgg tttaatgcta gatatgccga
     8521 caaagaatcc taaaacacgt aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg
     8581 aagcagaagg attcatctat ccatttcgtg gatatgtaga acacgtctat aatcactcag
     8641 caatcattcg cattgaaaac acgatggaat gtgataagtg gttagcgaaa agcaaagaga
     8701 atttagcagt ggtgagattg gtggacatgg aagttataaa atcttaattt tgctttttta
     8761 caactatcct acaacaatga gcgttattcc acaactggaa ggagaagagg caattgtgaa
     8821 cattttagag aatatagata ttaagcaaac aaggaaaaat gctagacgat tactaaaaag
     8881 atacagaaat ttagaacgtt tggtcggacc agtgaaaata gacttttccg tgatgactgt
     8941 taccaaaaat ttgaaattca caattgacag tcaaaacgaa gaaatcattg aagcgataag
     9001 tactcgagat tcggttatcg aagcattaac acggctaagt agaatccatt tccaggtact
     9061 ttattatagt tattgttttc ctaataagat gtcaatgtat caaataggag aaaaattagg
     9121 atattccgat agaactatcg agagaatgaa ggcagtagct ttggttgaat ttgctgaggc
     9181 gtataaatca ggagaactca tttcacgtac aaaataaaaa aagtcggatt cctccgacta
     9241 tgaataatat ttccgacata agtattatat cataattggg ggaatcagag gatggtactt
     9301 ttcgatgtaa agaaatatga gacaccaagc gcgaaagatg ttgatatgga acgcacaaaa
     9361 cataatgtcg ctgtgtttct ttcagcatat ctatcagcta gatgtagagt agggcaacat
     9421 cgagaaccaa aagtgacagc atcttattcc ttggttccac cttctacagc tgatcatata
     9481 tttgaagcag aaagaatgct gatcgataaa gaagaagcac aggaagaatt tgagtatctg
     9541 cataaattgt ttgttcgagg ctattcagcg atacagcatc cgcataagcc tgatgtgact
     9601 gaaaggcgca agaagatatt ctatgatcgt tatatcaatg gtctgcccat ttatgtaact
     9661 gctcaaagga ataatactag cgaagaatcg gttaaagtag aatcaaacag aattatcatc
     9721 caatttgctt catcgttaga actggttgct ttcaagtagc cagtttttac actttttata
     9781 cccttttatt accaatttgg tttccatttt ataccttttt tataccaatc acttacctat
     9841 tcaacgttgt attatgatag tgtcgaaaaa ttaggaaaca ggatcgacaa aataaaatgt
     9901 aagggaggaa atctccctca tcgtttaatt aagcttcgat aggcagcaac ggaaatatta
     9961 agaataagga tgtgaatttc aactccttct aaattgttct tattatctat catccgttgc
    10021 tgtctattgt ttttttaatt attcacacga taactaaagg taagtgaaga gaaatgattc
    10081 cattaataat ttcaattttt gcgctttgtc ttaatgtata tatgattgga tttaaaaatg
    10141 ggcaaaataa aaaatagtag cagccaagaa taattttata gtgtcactgt ggcggaaagg
    10201 gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa ccatgcaagg ttcgattcct
    10261 tgccagtgac tttggcaacc gaggcatcgg cggtttaaaa atataggggt gcgcaatttc
    10321 gtacgcgttt tgtgcatcgt gtaggttgct attacataac tggttaggtt agattgagat
    10381 ttgggattcg gtacaaatga atcgtcaaat gtctcaagca caggatcgga aacgtccctg
    10441 cctgtgcatt acatattaga tcactctttg agtggtcttt ttctttacat aaaggaggct
    10501 acataatgag aaactactgg tatgtatcgc taactaatga atatcctcga accattgatg
    10561 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
    10621 tggggagaga agcagaacca tgtgagattg atgcgtgcaa gctcgtttat tgcggtcatg
    10681 gtttctatga tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg
    10741 caagaaatgg cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt
    10801 aaagatattg attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact
    10861 caacctaaac agaatgtgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg
    10921 tgatgaagcc aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc
    10981 atctgaatta aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag
    11041 cgctgaattg attagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc
    11101 tcctattggt aatcaaaatg ctaaaggtaa caaaggaaat agccgagctt ctccgccagt
    11161 gggtaataaa aacgctttga aaacaggtga gtatgaaaca atattttttg acacgttaac
    11221 cgataatgag aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga
    11281 agaaatacga cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga
    11341 agctggacta aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc
    11401 gattgaaaaa aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat
    11461 tagcagaaaa aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg
    11521 gattagcaac cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata
    11581 cagaactgat ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg
    11641 cggaaataat tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa
    11701 caaacgggcg gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg
    11761 ccattgatta ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc
    11821 ctgatgaatg gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc
    11881 gttctggtca aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa
    11941 cgtgtcgacc ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg
    12001 tactttgggc agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt
    12061 ggacaaagac caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa
    12121 cagcgactaa accagaaagt atgcaaggtt ttcacgagga ccatatgttg attgtggtag
    12181 atgaagcttc tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt
    12241 ttgataataa gctgttgatg tgtggaaacc ccaataatat tgaaggtgtc ttttatgatt
    12301 cccataattc agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac
    12361 gtacaaacaa agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg
    12421 ctcgtgtccg tatttttgga gaatttccga aaggtgcgtt ggattcattt atcagtcttg
    12481 aaacagttga attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaaagg
    12541 ttgctcatat tggtgtcgac gtagctcgat atggtgatga ttctacgatt ctctttccta
    12601 gaattgctac cagggcattg gagtatgaga agtattcaaa acgtagcacc atggaaacaa
    12661 caggatatgt catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag
    12721 tgatgattaa agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac
    12781 ttatagaaga caaacattat ccttttgagg tgtttggagt gaataacggt tcaacatcag
    12841 aagacgattt ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag
    12901 aagaaaatat gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata
    12961 gttcgttaat caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac
    13021 gtttagaaag taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg
    13081 cactggcttt agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg
    13141 tgagcttttg attagtacag tattatcttt agagagatat aagaagctac gtgttaaata
    13201 tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaag atatgaaacc
    13261 attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga
    13321 taatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat
    13381 gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga
    13441 ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa
    13501 aaatgatata gaacttccga cagttccaga agcttttaac aaattaacaa gcaatcttga
    13561 ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt
    13621 agctttccga ttgctagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa
    13681 tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta
    13741 tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct
    13801 gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga
    13861 tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg
    13921 ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga
    13981 tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat
    14041 gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagcccc
    14101 tactgcaaaa gctgaatatg tcacaaaaaa tttagcaaaa gatttcaatg aatatcatat
    14161 ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt
    14221 gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa
    14281 aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc
    14341 tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt
    14401 ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc
    14461 atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga
    14521 aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg
    14581 cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg
    14641 atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg
    14701 aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt
    14761 atccagaaat gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg
    14821 aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat
    14881 tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat
    14941 ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct
    15001 tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa
    15061 acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat
    15121 ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac
    15181 tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca
    15241 ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca
    15301 aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa
    15361 aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag
    15421 tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca
    15481 taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac
    15541 caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg
    15601 agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt
    15661 tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct
    15721 attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac
    15781 gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc
    15841 gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat
    15901 tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt
    15961 aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt
    16021 gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg
    16081 ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt
    16141 gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat
    16201 gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa
    16261 gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa
    16321 tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa
    16381 gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct
    16441 ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt
    16501 gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat
    16561 gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt
    16621 ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa
    16681 gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa
    16741 catctttaaa taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac
    16801 caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga
    16861 cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg
    16921 gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt
    16981 ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa
    17041 atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa
    17101 aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga
    17161 ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa
    17221 gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac
    17281 tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa
    17341 caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt
    17401 ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg
    17461 caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg
    17521 caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg
    17581 cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct
    17641 tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg
    17701 aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga
    17761 tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt
    17821 gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat
    17881 cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac
    17941 gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa
    18001 atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa
    18061 aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg
    18121 cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag
    18181 ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac
    18241 ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat
    18301 tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg
    18361 tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac
    18421 atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac
    18481 tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt actatcctag
    18541 tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa
    18601 atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa
    18661 tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg
    18721 taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg
    18781 gtcgaattgt tggagaagtt ataaacaatg aacattattt taaatatgta gaagaaggtc
    18841 ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc
    18901 ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga
    18961 aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc
    19021 tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc
    19081 gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatgtttct
    19141 ttcttaattg atattgcatt gattgataat aaccctaata gaaatttaat taatgaatta
    19201 gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat attatttcca
    19261 aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac attagaattt
    19321 ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa aaccggaatt
    19381 atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa ttttccggta
    19441 tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc taattatgaa
    19501 acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt tggtggtgca
    19561 gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat tactggaggg
    19621 aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt
    19681 gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta taaaggacag
    19741 tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag ttttactcat
    19801 ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta ttcagttgat
    19861 gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa agtagcaatt
    19921 ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat caaacaacga
    19981 ttaaattaac gaaaaaagac gaagaaggta aatatgagca agtacaattt aaatctgctg
    20041 aatttttacc aggaacagtt gtagaagatg ctgcaggagt tatggaagaa atgcaaacag
    20101 cgactgataa gcaatcagtt aaaaaagctt taagtcgtgc ttattcattt attgcagata
    20161 ctttatttga gggacaattt actggtgaag attattgcaa aggaattgat gctcgtgaga
    20221 ttgcctcatt gacaggaaaa ttattgaagt ctgttactgc aggttttgat gaaacttata
    20281 cagagacgaa aaaaaagtaa gtgaggcgct caagtcacct tcatttaagt attcgattac
    20341 ttaccgagaa ttagatataa agacgcaatt actcgaagca ggttggacgt taccagaaat
    20401 tgaacgtact gacttggatg agttaatgcg tctttttgct tttagagatg cggtaaaaga
    20461 gcatgaagat gttgagtact atgataattt cactcaattt taggaggtga tactttgaat
    20521 aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc cggtatgaat
    20581 aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt
    20641 ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc tagcggagta
    20701 actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg agcggttgat
    20761 cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt tagtaattca
    20821 gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca aggacttcct
    20881 actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac aggtgattta
    20941 actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt cggtggttca
    21001 actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta
    21061 gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct ttctgctatt
    21121 gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca aggtaagatt
    21181 tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg cggaggactt
    21241 aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc tatacaaaat
    21301 gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa taaagcatta
    21361 gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag ttcaatggaa
    21421 tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt aagtaattta
    21481 tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt tcttgttcct
    21541 gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc aataataaaa
    21601 agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa gattatgggg
    21661 gcaatagctg ctgctaatcc atttgtttta attattggtg cagtcgttgg tgcaatagct
    21721 gttttcggtt actttatggc aaccaatgaa gagcttagaa acaaggttat atctatttgg
    21781 aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg gggaattgat
    21841 acttggaatt ctgctaaaga aatggcatca aatgcagttg agggtgtcaa agatgcttgg
    21901 tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg ggccacaggt
    21961 ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg
    22021 tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc agccagagaa
    22081 aaatggaatg gaatcaaagg ttctattatg tcagttgctg gtccattgat tacaggtata
    22141 aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa tttagttgag
    22201 attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt tttgtttatt
    22261 acctccatga tttctggtgg atgggaagaa acaaaaaata atatgattgc tgtttggaat
    22321 aatattaaag aaagtgccat aaatatttgg gaatctataa agaatatatt tgtaagttat
    22381 tttacaaata tttattttgc tgcactcaat atttggacag ggttcaagct tacattgata
    22441 aatatttgga atgaagtggt aaatcaagct aaatcgattt ggttaaacat aaagtatttt
    22501 tttattaatc tttggattga tataaaatat tttgcaattc agaaatggat tgaattgaag
    22561 ttttctatta ttcaaacttg gatc
//
LOCUS       DAZKOK010000037        22530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000037 DAZKOK010000000
VERSION     DAZKOK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.037"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(269..2161)
                     /locus_tag="ACG90W_001264"
     CDS             complement(269..2161)
                     /locus_tag="ACG90W_001264"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477639.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2158..3885)
                     /locus_tag="ACG90W_001265"
     CDS             complement(2158..3885)
                     /locus_tag="ACG90W_001265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477640.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3901..4551)
                     /locus_tag="ACG90W_001266"
     CDS             complement(3901..4551)
                     /locus_tag="ACG90W_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0477641.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4755..5051
                     /locus_tag="ACG90W_001267"
     CDS             4755..5051
                     /locus_tag="ACG90W_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0477642.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5223..5843
                     /locus_tag="ACG90W_001268"
     CDS             5223..5843
                     /locus_tag="ACG90W_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0477643.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5935..7101)
                     /locus_tag="ACG90W_001269"
     CDS             complement(5935..7101)
                     /locus_tag="ACG90W_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0477644.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7113..7466)
                     /locus_tag="ACG90W_001270"
     CDS             complement(7113..7466)
                     /locus_tag="ACG90W_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0477645.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7594..8616
                     /locus_tag="ACG90W_001271"
     CDS             7594..8616
                     /locus_tag="ACG90W_001271"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0477646.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8635..8796
                     /locus_tag="ACG90W_001272"
     CDS             8635..8796
                     /locus_tag="ACG90W_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477647.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9055..9489)
                     /locus_tag="ACG90W_001273"
     CDS             complement(9055..9489)
                     /locus_tag="ACG90W_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477648.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9599..11458)
                     /locus_tag="ACG90W_001274"
     CDS             complement(9599..11458)
                     /locus_tag="ACG90W_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0477649.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11459..11599
                     /locus_tag="ACG90W_001275"
     CDS             11459..11599
                     /locus_tag="ACG90W_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0477650.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11576..12736)
                     /locus_tag="ACG90W_001276"
     CDS             complement(11576..12736)
                     /locus_tag="ACG90W_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0477651.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13043..14467)
                     /gene="araA"
                     /locus_tag="ACG90W_001277"
     CDS             complement(13043..14467)
                     /gene="araA"
                     /locus_tag="ACG90W_001277"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0477652.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14483..15181)
                     /locus_tag="ACG90W_001278"
     CDS             complement(14483..15181)
                     /locus_tag="ACG90W_001278"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0477653.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15138..16799)
                     /locus_tag="ACG90W_001279"
     CDS             complement(15138..16799)
                     /locus_tag="ACG90W_001279"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0477654.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17017..18417
                     /locus_tag="ACG90W_001280"
     CDS             17017..18417
                     /locus_tag="ACG90W_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0477655.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18669..19748)
                     /locus_tag="ACG90W_001281"
     CDS             complement(18669..19748)
                     /locus_tag="ACG90W_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0477656.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19834..20793)
                     /locus_tag="ACG90W_001282"
     CDS             complement(19834..20793)
                     /locus_tag="ACG90W_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0477657.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20805..22316)
                     /locus_tag="ACG90W_001283"
     CDS             complement(20805..22316)
                     /locus_tag="ACG90W_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0477658.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22338..>22530)
                     /locus_tag="ACG90W_001284"
     CDS             complement(22338..>22530)
                     /locus_tag="ACG90W_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477659.1"
                     /translation="ITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIFLFPPFIFI
                     FAGGVWKLVSIILTIGNER"
BASE COUNT         6416 a         4775 c         3984 g         7355 t
ORIGIN      
        1 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg cagatgcaag
       61 cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag
      121 atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt ttgagctaat accgaaggac
      181 agctgattga acaccgttta ttcggaaaaa gggactgtga cacgactttt gtcacagccc
      241 ctaaaaaaaa taataaattt gtactattct atgaagcttc ttcttcagaa aactgactgt
      301 tataaagatc tgcatagaat ccgtcttttt ccatcaatgt atcgtggttt cctgtttcta
      361 cgatcgaacc atgattcatc acaataatgt tgtctgcatc gcgtattgta gacaagcggt
      421 gagcaacaac gaaacttgtc cggttttcca ataggcggtt catcgctttt tgaatcagta
      481 tttctgttcg tgtatctaca ctagatgttg cttcatcaag gatcaggaca tctggatttg
      541 ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc ttgcgaaata ttgcttgcat
      601 cctcgtttaa tacagtgtta tatccttctg gcaatttacg aacaaaatcg tctacgtgag
      661 cagctttagc agcctcaatg atttgttcgt ctgttgcatc gtcgttccca tatttgatat
      721 tgtcatagat acttcctgta aacagccaag tatcttgcag taccattgaa aactgtgcgc
      781 gcaattcatc acgtgaaaga tcacgggtat ctacaccatc gtaacgaata ctacctccag
      841 aaacatcgta gaaccgttca agaaggttga tcagtgtcgt cttccctgca ccagtcggac
      901 ctacaatagc gaccatctca ccaggtttga catccaagtt aaaatcagtc attagcaagt
      961 tatctggtgt gtagccgaat tgaacatttt caaaacgaac tttgtatggt gtatcttttt
     1021 cttctggaag gccagatttt tcattggaca tctcttcttc atccaacact tcaaataccc
     1081 gttcggctga agcaactgtt gattggatag tgttcatcaa acttgcaagt tgagtgattg
     1141 gttgagagaa ctgtgttgta tattgcaaga acgcttgaac atcccctaat gtcatgttcc
     1201 catttgcaac ttttactccg cctagtacag cgacgaacac atagcctaga tttttaacga
     1261 aattcatcaa cggcatgatg attgccgaaa taaattgagc tttccaacca gcggcataca
     1321 gattctgatt ttctttttca aatacttcct ggtctttttc agtatgattg aatgttttaa
     1381 caatggtatg accaccatat gtttcttcta cttggttatt caataaacct aaactttttt
     1441 gctgatctgc gaaaaacact tgtgatttcg gcgctacgat catcacgatg atcaagctta
     1501 aagggatcat caataacgcg actaacgtca attgccagct gattgaaagc atcatcacga
     1561 tgaccccaat caatgtgact gcactagtta caaattgagt caaattttgc tgcagcgtgc
     1621 ttgcgatgtt atccatatca ttgattgctc gagacatgat atccccattt gtatgtgtat
     1681 cataatagga aatcggtaca cgattcattt tggcttccaa atcatgacgt aattcataaa
     1741 ctgttcgttg ggaaacacgt gtcataataa attgctgtaa aaagttgaat acagcagaaa
     1801 tcaaatacat cacgattact gtcgcaatga tctgcgcgat tttatcaaag tctatcggaa
     1861 attttgttac ttgttggcct tgttgcatct gttgagcgcc agccattaca cctttgaata
     1921 tttctgtggt tgctttcccc aagacctttg gtgtttggat ttggaagatc acggctgcta
     1981 ttgctagtac aaatacagcg atgatcgcaa ccatacgttt agacatatag ccaaacaatc
     2041 ggcgtaccgt tccccagaaa tttttaggct tttcaccttt tacaccgaga tttctgccag
     2101 gtccaccatg tcctggaccg cctgctggtc catgagaggg acgtgtgttt gttttactca
     2161 tgctaagtcc tcctctctta gttgtgaatg aacgatttct tgataagttt cgtttgttgc
     2221 taatagttct tcatgtgttc ctttgccaac cagttttcct tcatctagca caaggatcat
     2281 atctgcatcc attactgtac ttactcgttg agcaactaaa acggtgatac tttctttcat
     2341 attcgttttc aatgcttggc gaagattggc atccgttttg aaatccaacg ctgagaagga
     2401 atcatcaaac acatagatat ctgctgtttt gactaatgcc cgtgcaattg ccagacgctg
     2461 tctttgtcca ccagagaagt ttcctcctcc ttgttccaca tggctatcta agccatcttc
     2521 catctctgaa acaaaatctt tagcttgtgc gatttctaat gctttccaaa tcatttcatc
     2581 tgttgcatcc ggcttaccat actgcatatt gtctcggatc gttccagaga acagaactgc
     2641 tttttgagga acaaagccca ttaattctcg caaattatat tgagtcatgt cactaatgtc
     2701 cgttccattt atttggatcg aaccagattc gatgtcatat aatcgaggga tcaaattgac
     2761 taaggtcgtt ttacctgaac cagtcccccc tatgattgcg acgatctcgc ccgacttggc
     2821 ttggaaatca atatcttcca atgcaagttt ttctgctcca tgatatcgat aattgacatg
     2881 attgaaggcc aatgttgcgt tttttccagc aaaagaaact gttttaggat tttcagggtc
     2941 atggataccg attttttcat ccaacacttc attaatacgg tcagctgaag cttgtgcacg
     3001 aggcaccatg ataaaaatag cggaaagcat cataaagctg atcaagatct gcatcgcata
     3061 agtcataaat gcaatcagat taccgacttc caaagtcata tctgctatgt agtgtccacc
     3121 aaaccaagtg attgctacgt ttgtaccgct catgatcaat gtcatcattg gcatcatcaa
     3181 tgcgacgatc gtattaactt tgattgccgt atttgtataa tctttattcg ctaaatcaaa
     3241 acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc actctgaccc ctgtcaatcc
     3301 ttctcgaaat accaggttta aacggtcagt tttcttttgc atacttttga ataatggaac
     3361 agcaaagtac atgattccgc cgatcaaaat aatcattact gggatgacaa ctaagaagat
     3421 ttttgtcaac tgatgatctt tttgataagc aagtatgctc gcgccaatca acgtgattgg
     3481 tgcattgatc atcatacgta aaaacatttg tgtaaccatt tggatttgag tcacatcatt
     3541 ggtcgttctt gtgatcaaag aggccgttcc atatttatca aacgcatctt ttgtcagact
     3601 ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc agtttctgtg attcacgagt
     3661 agcaaaaaat gtatttgcga tagcagctaa gatactgatc aaagaaaagc cgatcatcac
     3721 aaaacctgta tgccaaatat aatcgacatc tccttgcgct acccctttat caataatatt
     3781 ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg atcaccatga aaactgcagc
     3841 tgcaattgct gaagtcaagg agatccgttt tacgattttt agcatctagt tcccttcttt
     3901 ctatttatat actccgtttt tcagccaaga aagtttcaaa ttgaattcac tgattgcttg
     3961 ctctactgag taatcttcag atgttgaaaa taagcgatac gaatgcgcaa ttgttgtaaa
     4021 tacagtattc atcactaatt ggatcagtaa ttccaattca tgatcatttt ttactttcag
     4081 cttcgtccga tcgatcaatt caagcaactc ttttgcatct tgtttgtgtt ctttatgtgg
     4141 atcgtgaggc gttttttggc gactcgtatg aggagcgact tttcttgagg cacggtaatc
     4201 catgttcata aacaagttac gataaaaaga agcattctcg ccttccaaaa tctcataaat
     4261 cattttagaa aaataggatt cgaatccttt gaatagatca ccatcggatt ctttcattag
     4321 ttgtaacata tctttagagc tgtcacgtct cattgtctca aagtaataaa aataaagatc
     4381 ctctttatct tcaaaatact gatagaaact gcctctaggg atatccgcta atttgacgat
     4441 attagcaatc gaagcttctt ttaagggtac tctagaaaac tcgattctcg cagcctcgat
     4501 tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc tgtgtcggca tatggatcct
     4561 cctttctttc aagatgacac attgtcatat gacgctgtgt cattataaca cctcctacct
     4621 gttttgcaag acatttgcat agtttttttc attatcatcc gatacattaa aaaagtgata
     4681 agaggaatcc ttccatttga attaatgaga gttgcccatt aaaatagcca catactaaac
     4741 aaaggatgag ataaatggcc caatcaatta gtgtccaaga cttcaataaa ttgcctctcg
     4801 atgctgacag catcgtttta gatacaagaa ataccttaga ttacacaaaa aaccacttaa
     4861 gccactcgct ttctatcccc gctgctatct tgccaagaca attaaaaaat ctagatccaa
     4921 agaaacttta ttatattcta agctatacag gcaggcgatc agaagcatta gccgatcaac
     4981 ttactgctaa tggttttcgt gctgtttata tcattggtgg gatggaagct cttcagtcga
     5041 gtgttgcttg aatattgctt gttagattca aaagaggctg tgacaaaaaa tcgtgtcaca
     5101 gcctctttta taccctgtct ttttttagtt atttattctt caaccttatc aatatgactt
     5161 gatagccttt tctttttctc atagttataa caatcatagc aataaaagga gaaggtaagc
     5221 atatggcatc tgttttagta gtcaaaggtc atcccctaac tgcagaagaa tcgcgcacag
     5281 ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa tcatccagat gatgaagtga
     5341 ctgttttgga actttatcgc gatgatattc cggaaatcga tgaagaatta ttatcaggtt
     5401 gggaagcatt acgtgctggt gcagaattca cttccttatc agaaagtcaa caacaaaaaa
     5461 ttgctcgttt caatgaattg actgatcagt tccttgctgc tgataaaatc gttatcgcaa
     5521 acgccttatg gaatctaaat atcccaacaa aattaaaagc ttggttcgat accgtcaatg
     5581 tagctggaaa aacgttccgc tatacagaaa acgggccaga aggattagtg acaggtaaaa
     5641 aagcactgca catccaatct aatggcggtg tatacaacgg acaagacttt gcctctcaat
     5701 atgtaaaagg tattttaaac tttgtaggca tcgatcaagt agatcagcta ttcatcgaag
     5761 gaatcgatta tgaccctgac cgtgcagatg agttgatgca aaatgcatta gataaagctg
     5821 ctgctttagg taaatcattt taaaaattga gataaatgca tacaaaagag acagaggaaa
     5881 aaatcttcaa agcagcattt gtaccttgga tattttccca tctgtctctt tttgttattc
     5941 tttctgcttt ttcaacggat tttcttcata aagagaagag attgttgtgt accattcaaa
     6001 aacatgagta atcactactt tagctgctgc ataaacagga atccccaaga tcacaccgat
     6061 gattccgaac aatttcccag aagtcagcag aactaataaa atcgttactg gatggattgc
     6121 taactgattc cctagtacta atggagagat caagcgtcct tctattgtct gctctactgc
     6181 aaaaacgatc agtactttga tgatcatgaa tggacctgct acaatggcta aaaagatagc
     6241 cggaatcatc gctaaaaatg atcctaagta agggatcagg ttcaaaaaac ccgcaataat
     6301 ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa ccaatcataa acatgattgc
     6361 cacagcaaaa gccacagtta gttgtcctct tatataagaa gaaacttgac tattcatttc
     6421 actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt aaaaatttga caaaataagg
     6481 tgctaattgt ttaccgtctt tcaatagata aaataagata aacggcatcg tcagcagagc
     6541 cactaaaata gtcgctaccg cgccgacaaa acttccaaga ctctgaactg tactcttaga
     6601 tatatcttgg atcatatcgc ctgccgaatt catgaactta tcacctgctg tttccaactg
     6661 ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg gttaatttat tatcaatcgt
     6721 atctacatat tgagggaagt tactgatgaa gctcactgtt tgttcctgaa tcttagggat
     6781 gatcactacg ctcccccatg cgattagtgc aacaaccaaa acaaacagcc caatgatact
     6841 gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact accggattca tcaagtaata
     6901 caagattcct gctaaaatga taggcagacc tacgattgct aaaaattgcc agattggtgc
     6961 aaacaaatat gaaacttttg taaataaaaa gacgatcagc aatattaaca atacaacaag
     7021 taaggctgtg actacttgat tatttaaaaa ccagcgccaa aaccacgata ccggtctgtt
     7081 cttcttattt tttccttcca taattcctcc tgttagttat aaaaaatttg gcttcccaaa
     7141 ccaatagcag gcagtctatg cggatgaaga tagattccat ttgcaatttg tccttcttct
     7201 ggacaagcat caaaaatcaa tgatacgtga gctactgtct ctaaattccc attggccatc
     7261 ggaccgacat aatcgatcgt atatacctga tcatcaaaag caattgttcc gcctgctttc
     7321 aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg aatattttcg taaggcagct
     7381 gttgctgttt tatcgaacaa aatcagcatg ggttcttcct tgtttattgc ttctgtccca
     7441 atttctgtaa tctttgcctg aatcattgca ctctctcctt taattgcttt caaaaacagt
     7501 ataacacatt ctcattcttt agccgtgtgg aataattatg tgatagaata aaaacgaaaa
     7561 ctatttcatt acaattagga ggaatcagct catatgatcg aaaagataat tttaggaacc
     7621 tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc ttgatacaga aaaagaagaa
     7681 ttagccaacc tttcattagc tgttgaagaa aatagcccta cctatgtagc aaaaagtaaa
     7741 gctggcaatc tttacacagt tactagtgtg gatggattag gtggtgccgg tgcgtacgat
     7801 aaggattttc attttttgaa tgcggtaaca gagtctggcg ctcctttgtg ttatgtagcg
     7861 gttgatgaag cacgtcaatt actatacggg gcgaactatc ataaaggtga agtaaatgtc
     7921 taccatatat taggagatgg cgaactgaaa gcagcagatt caatattcca tcaagaagcg
     7981 acaggtcctc ataaaaacca agatcatgcg catgtccact atacggatct gactcctgac
     8041 caacgattag tcgtatgtga tcttggtaca gatcgtgtgt atacttatga tgtgtccgaa
     8101 aacggcaaat tgaatcttgt aactaccttc actgcagagc caggaacagg ccctcgccat
     8161 cttgtctttc atccaaatgg acaattcgct tatttatttg gtgaattaga cagcacagtc
     8221 cgcgtgcttt cttataataa agaaacaggt gcttttgaag aattacagaa aatatcgacc
     8281 ttgccagaag aattcgacgg tgaaaatgga ggggcagcta tccgcatttc aaatgatggc
     8341 aagttccttt atgcttctaa ccgaggacat aactcgattg ctgtattcgc agtttttgaa
     8401 gatggttccc acattcaaaa caagcaaatc atctcaacag aaggggattt tccccgcgat
     8461 ttcgcactag atccaagcaa taattttgtc gtgtgtgcca atcagaacac cgataatctg
     8521 acattatatc gccgtcatcc tgaaacagga ttactggaat tactgaaaaa agatgtctat
     8581 gtaccagaat gtgtctgtgt ttattttgat cgttagtaag aaaaggagaa tgaaatgaat
     8641 cagcaagacg tattagacag actacgggaa gaactaaata ttcctttctt tgatgggaaa
     8701 atcgaagata aagagtatag cgaagaggaa tatcagaaac tcaaagccga tttacagaac
     8761 tactttgaac aatatgtaca gaatgtagaa aactaatcag ctttccatac tatctgaaaa
     8821 gaacatcagg acaagacttt tccgtctatc ctgatgttct tttctacaaa taaaaaaccg
     8881 ccgagatggt tagactcgac ggaaaaggag tatttactcg attttgagta agtacaaggt
     8941 tgttgttggt tataggtatg tcattatcat aaaggatttt gatcactcta tcataaaggt
     9001 aatattacat ttatattaga taactctagt ttttattgtt atcaaagatt tcttttattt
     9061 tttcaacaac aaattagtca ttttttggat ggcagctgtc aattggtctg tcgcttggtc
     9121 ttgttttgct tcagatgtat tgaatagatc ttcatctacc cctgattcaa gatcgatact
     9181 ttggatcatt tcttcacagc ctgctacata tgaaagataa gctttttcaa aatttttatg
     9241 aatgcccatt gcttttgctg gtggacgcaa agtactgact ttcttaagca ttgcccgata
     9301 tttttttgtt ccttcttcaa ataacaggat gatctcttca agagtcgatt tgtccagttc
     9361 acttgttttt ccttcatcaa ttgcgtttct gatcacttca tatttcggat gcattccttc
     9421 tccgattgct tctgtttcct tgacgatttg atcgatggac tgcacataat agccaatatt
     9481 cggtaacatg ttcgttttcc tactttctcc attgttgtta ttatttattc tacttgtttt
     9541 tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt ctatacagct cgccattctt
     9601 attcgtgctc aaagaaacca tctgctaaac tatattgttt ccctgcgaca atgtcatatt
     9661 ggatcaattg atattcgtcg taaatctctt gttgcgagcg attgaattgc gcttctttat
     9721 accactgtcc gttttgatag tacagttctc gttcaaacgc cggaagttcc tgttctaatg
     9781 ataaaagcag ctgataaaag ccatttgttg tctgattcgt ctgttccatt agattcgctg
     9841 caaaataaaa cggactggtg attgcatcgt gagtcgtttg cttttctaat tctcctttac
     9901 tgtcgaacat aagaaattgt gtttcatgca atgtttgttt gtcatttttt gcttggatcg
     9961 tatctgaata gatccctgga agatgatccc cccaaaaaac aactaaagtc cttctagaaa
    10021 gattcttcaa ttcttccgtg aactgcttca gtgccgtgct gctataggaa atatcttgca
    10081 gataattctc taagtcaagt gtcccacttc catctgaagt ttttgcagga tagtctagat
    10141 tatcatactt tccatcgtag ggcatatgtg tctgcatcgt gactaaatga atgaactgag
    10201 gtgttttttc ttccttgaga agtgtcatta cttctttgta agctgactca tcagaaatat
    10261 atggattatt ctctatcgta tccgtatagg tcatcgtacg ctcgctgatg aactggtcaa
    10321 atcctaatgt ctgataaaca tcctctcttt tatacatcga agtattgtat ggatggatcg
    10381 ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat agaaggaagc tgatccattt
    10441 ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt aaataacgcc atagaaaaac
    10501 cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc atagttttga gaaagcatat
    10561 tgccactata tgtttgatca gctacttcat aataatctgc caatggctca ccagatactt
    10621 ctacgccgtt taatcgacta ggatctgaga aactttcact catcacgaac acgatgttcg
    10681 gcgactcttc ctctacagtt ttattttttt catcggctag cttctggtat ttttcagtga
    10741 tttctttgat cttttctttc gaatatcctt caggttcatc cattggttct accttcagat
    10801 tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt cattttctgg ctgtaaggta
    10861 tccatagtgc cgttttatta tatgcttttc gcaacaaatt atcgggattg ttgaaattac
    10921 tgatataccc tagcagtcca attgccacaa gcagtgtcgt taatcggatc aattggaatc
    10981 gccttccttt tttcaggctt cgaaacatgt accagcaaaa caatcctatc acggttcctg
    11041 cggcagctaa gatcactgta aacagcatag tgccggtcat atctttcagc aatccgattt
    11101 cagtgatcat cttcaaatcg tctggataaa tcggttcttg ccgataactc attttcatat
    11161 aatttgcaaa acctaataca ccgatggcta cgacatacgt aagggaacct aaagggacag
    11221 atcctgctag tgcaatcaga aaaataagaa taatcagcaa gacgagacag gctaagaaga
    11281 atttttccgt atgccaagaa aaagcaaatt tcaacgcaag gtctacggat agatcatttt
    11341 gacaccactg caaaaataaa ttactaaaaa caacaagaaa aaagatactg atgattgctg
    11401 ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct gcttatatat cttttcaaat
    11461 gcttgctcct tcatttctat caataaaaga atgttgcttc tcttatttta gttgtacgaa
    11521 attcgaaagt aaagaacgga aagaagaatt aatgaaatcc aaatattctc tttctttatg
    11581 gcctttctgg tttctttaat aagcggacct ttcgtactcc agaaattttt tctagctgtt
    11641 gtttcacttg attgagctgt tcaaaatcag tactgtctat gtcaatcaat atgtagacat
    11701 gctcattctt atggttacgc gatatatctg cgatcgctag ctcgttctca ttcagcagag
    11761 aaaatatctc tgctaatata ttggttttct tttgataaaa tatagtgaat cgaaacggcg
    11821 cttggaaaaa cattcgtgcg gatggataat tgaccgcttc tctaaccgta ccgaacaaaa
    11881 gataatcttt taaggcttca actgccaaac gtccgctgtc tgccaaagct tcctgagtcg
    11941 atccccctag atgaggcaac ataagtatac ggtcattttc taagaactct tcttctggaa
    12001 aatcagtcaa gtatttagcc agatgttgtt tttccaatgc ttgcataaca gcatatttat
    12061 caacaatttc acttcttcct acattgataa gtacagcatc ttttttcatt ttttctatat
    12121 tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg cagcatgact acgatgtaat
    12181 ccgaggtaga aagaactgtt tctaaatctg cttgccgaac ataatccaac tgggcatcgc
    12241 ggatagaata acccaagaca tccatcccta agctatagca acttagcgct acttttgtgc
    12301 ctatcgctcc aagaccaaga agaccgatgg ttttgccttg caattctcgt cccacataag
    12361 cacttctttt gttttctgcc tgttctaaaa tattaggacc tgttagtgtt tgtaccatac
    12421 gacttgcttc gattatcgga cgggaagata agagcaaaca gcacaaaaca agctctttta
    12481 ctgcattagc atttacacct ggtgtattca tgacgatcgt cccgttttca gaagcttttt
    12541 caacgttgat cgtgttcacc ccaacaccag ctcttgaaat tgctagcaaa tctggtgtaa
    12601 gccattcatc tttcactttc gtactacgaa taactattcc ttcggctggc aaatcatcta
    12661 ttattttgaa atattcccct tgtaactttt ctatctcttg ttctggaata gcatcaatca
    12721 aatgaacgtg atacataaaa ccccctcctt actaacaagt atctcgaaat ttctgcaatt
    12781 agacaactgt ttaccatttg acttcctaga agcaaaaaga gcagttagag cagagtaggc
    12841 tgagtgataa aaataaggct atgacaagtt actgccataa gccttatttt cgagagcaaa
    12901 atattgaggg tgagatatct tgataaatgg tgttccgtta gctgacattg ataataagac
    12961 taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc ttcttatttc ttaaaactaa
    13021 gtggcttttt cacaacctct ttttatctaa taaccactgt ttccaactca actaattttg
    13081 cccacgtttc aatttgatct gttgttagat ttagcgagac aacggtatga tgaccgccac
    13141 ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg tttagttttc cataacacac
    13201 gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa ggcttctact tcattgatca
    13261 gcagtttata atgcgtaccg aaatctgcca tcgatacgac taccccttct cctgctttcc
    13321 catcaaatac aagacgcgcc ggatcttctc tatctcccat tgataaagga gaaacaacga
    13381 ttcttggttt cgtcgcagca agcgtcggat ctacttccat catatgggat tccaagattg
    13441 cttcttggcc actagctagc tcgtaagtat aatcttccat gaatcctgta tctttccctc
    13501 gagccatgat tttcagcaac cgatcaatcg ctgcagtttt ccagtcgcct tccccagcaa
    13561 aaccgtatcc ttctgccatc aaacgttgta cagctagtcc aggtagttgt ttcattccat
    13621 acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc ttcttctaag aaacggcgaa
    13681 taccgatttc ctgctgagct tgtactttta catgttcctc ccatgtcttt tcttcataat
    13741 caccatagtc gaaggtatac aattccttgt actcttcaaa taattgatcg acttcttcgt
    13801 ctgatacgcg atcgattact tgtacaagat ctccgatacc aaaataatca acagtccaac
    13861 caaattgaat ctgtgcttcc actttgtctc cttctgtgac acccacgttt ctcatgttgt
    13921 caccaaaacg agccactttt attccaaaac tttcattata ggctactgca acatccatcc
    13981 aatcagcaat ctctttttgg atctcaggtc gtttccagta gccaacgacg attttatttt
    14041 gtttatttaa tcgagcattg atgaacccat attcgcggtc accatgagcg gattgattta
    14101 aattcatgaa atccatatcg atcgttttcc aaggaatgct ttcgttatat tgcgtagcta
    14161 aatgaagcaa tggtttttgt agaagctttg tcccgcggat ccacattttt gctggtgaga
    14221 aagtatgcat ccaagtgatc acacctgcta cttcttctcg ataattgacc tctttcatga
    14281 tttttgtgat cgtatcagca gtaactgcta attcttgaag gcggattgga taaggcaatt
    14341 ggccattttc attcagttca tcgaccattt cttgtgcatg tttctttact tctcgcagtg
    14401 cttcttcccc atataaatgc tgtgatccta caacaaacca aaattctttt tctccaatat
    14461 tcaacataat tagccactcc ttttatttgt tttgtccgta atacgcattt tttccgtgtt
    14521 ttcgtaaata atgtttatct aaaatccgtt gaggcagctc ttctgaaaat gaattgagct
    14581 gtctggtaaa taaattcatc ttgcatactt catctagtac gactgcgttc attaccgcac
    14641 tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc cctggaattg
    14701 ctaagggatc aattttgcgt tctttgaatg tttctatgat aacttttcct gtttcatatt
    14761 cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc gatccataaa
    14821 aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt gcccagatcg
    14881 tcgcccatgt cgagtgagta tggacgattc ctccgatttg aggaaatgaa cgatataaaa
    14941 cagcatgagt aggcgtatcc gaagagggat tgagtttacc ttcaatgata tgattgtcca
    15001 agtccacaac gaccatgtcg tctgctgtca tctcttcata actgactccg ctaggtttga
    15061 taacgaaata tcgtgtttca ggatcaaaag cgctcacatt tccccatgta tatttgacga
    15121 gtccgtgttt aggcaagtca agatttgctt ggtatacttc ttctttcagt tgttccagca
    15181 ttttctatca ctcctttttc ttgcaattga tgaatcgctg ttttttcaat gtcgatcccc
    15241 tcacggtaac gtctcataaa tatctgatag cctgctattt cttctgctga aggtgtgatg
    15301 gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga agttttctaa agacttccca
    15361 tgtcgatcca tcatataggc tgctaataac gcgattcccc acgctccacc ttctccggct
    15421 gtgtccatca ctgtaactgg tgcctccata gcagatgcta gaatccgctg tccaacttct
    15481 ggtgttttaa aaatcccccc atgccccact aaacgatcaa tttggacatt ctcagatttc
    15541 aatatatcca ttccgattct cattgcacca aaagcactgg ctaaatggat ccgcatgaaa
    15601 ttcgctaaat caaaacggct acctggtgta cgtacaaata atggccttcc ttcatttact
    15661 cctgtaatgt tttcaccgga aaaataacca taagaaagta atcccccgca atccgtatcg
    15721 ccttctagcg ctttgttgaa taataccgtg aataatttat ctctgcttat ctccataccc
    15781 aaagaattgg taaattcttc aaaaatactg acccaagcat tgatatccga agagcagtta
    15841 ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag tgaccatgtc gatttccgga
    15901 tggacatttt ctagttcttt ttctaatacg atcatagcga aggcggaagt accagccgaa
    15961 atgttccctg tccgtttggc tacactgttc gtagcgacca taccggttcc tgcatcccct
    16021 tctggcggac aaaccgggat tcctggtctc aaatttcccg tagggtcgat cagataagcc
    16081 cctgattcgc ttaacttgcc agcttcttct cctgctacta gaactttagg caaaatgtga
    16141 tccaagtcaa tcgctacgtg cgctttttcg attaactgac caaaaatatt tttcatttct
    16201 tgatggtagg ttttcgttga aggatcgata ggaaacatgc ctgatgcatc tcctacgcct
    16261 agtactttct caccagtaag ctgccaatgg acataacctg caagcgttgt caaaaaatta
    16321 atttctttca catgttcttc tttattcaaa atagattgat acaaatgagc gatactccat
    16381 ctctgcggaa tattgtattg aaatttttcc gttaatattc gttctgcttc ttcagtgatt
    16441 gtatttcgcc aagtacgaaa tggtactagt aactgttcct tgctatcaaa tgctagataa
    16501 ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg taagattctc gccatagatt
    16561 tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca aatatcttct
    16621 aatgaatagg tccagatacc attttctagt ttgttttccc actcataact accagcggca
    16681 ataggagtaa aagatgaatc aatcaataca gctttgatcc tagttgatcc aaactcaatc
    16741 cctaaagatg tacgaccatc tatgacatct aacatacttt gcgcatttac ttccttcata
    16801 atgatttaac tccctccttc tcgtcaaacc tgatcaaacc gtctttttag tagcgcttac
    16861 aatgattatt gtatactttt gtacgtacat gtcaagcggt tatttacaag aatacaataa
    16921 aaaacacttg ttttgtacgt acataagtta taaaatgaaa ttgcttacaa aatctgatca
    16981 accgccttct cgaaattcta gaaggagaga atacttatga aaaaagaaaa aaaagtttcc
    17041 tcatcgttta tttatttttt tggcgcattt ggcggcattc tatttggata tgatattggt
    17101 gttatgaccg gcgccttacc ttttttacag catgattggg gtttggcagg taaagcttct
    17161 ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg ctctttatct
    17221 ggtcttcttt ctgataagct tggacgacga aaaatgattc ttctttctgc acttattttt
    17281 atggctggtt ctgtcctttc tgcatctgcc cctcacaatg gttcttattt tctgattgca
    17341 gcaaggattc ttctaggttt agctgttggc gctgcatctg ccctagttcc tgcttatatg
    17401 tctgaaatgg ctcctgctag actaagggga agattgtcag ggatcaatca agtgatgatt
    17461 gcttctggca tgctcctttc ctatgttgca gattatttgc taaaagtgtt gcctgaaacg
    17521 atggcttggc gcgtgatgtt agggctcgct gctgtccccg cgctgatttt atttttcggt
    17581 gttttagctc taccagagtc accgcgtttt cttatgcaat ctggccgctt ggaagaggca
    17641 aaaagagtat tgaactatat tcggacacca aaagaagctg aacaggaatt cgaacaaatc
    17701 cagctgaatg tcaaacaaga aaagacaacc ggaacttctt ggcatacttt gtttttagaa
    17761 aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag tattccagca atttcaagga
    17821 gctaatgcta ttttttacta tattccgctg atcgtagaaa aagcgactgg gcatgcagcg
    17881 agcgatgcat tgatgtggcc gatcatccaa ggaatcattt tagttgctgg ttctttgctc
    17941 tttttggtta ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg cggtagcgtg
    18001 atgggacttt cattcatcct accagctgtt ttaggaactg tactggatgc tcatacaaat
    18061 tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat tttattcttg cacatgggct
    18121 ccactgacat gggtaatcat aggcgaaata ttcccactag ctgtccgcgg gcgagcatct
    18181 ggcctagcct cttcttttaa ttggattggt tctttcctag ttggcttgct ttttcctgtg
    18241 atgactgctt ctatgtctca agaaatcgtt ttcggcattt ttgggatcat ctgcttttta
    18301 ggggttttat ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt agaggaaatc
    18361 gaacaaagtg catctaaaaa aacttatcct aaacgtattt ctatccatca ttcttaaaga
    18421 ggttgtgaca gaagtggtca gcttcaagaa ataagaagga accgcttagt ttccagtcac
    18481 aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa tattgtgact tgtgaccagt
    18541 tggtaggaga acaccgttta gtggagaggt ggtgaaagaa acgttcagcc ttgagcgatg
    18601 acaaaagctc tcacaaacat tgcgacgagt aaccgtagga gcaataagag gcggtaaaaa
    18661 cccgtctctt atttttcgtt ttctttcact gtttcccgtt caactagtac aggttcatag
    18721 agaatatttg gctgtgcctg tcctttttca atcgttgtga cgatccaccg tgcggcatct
    18781 tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt ttttattccc taaatggctt
    18841 aaagaagaat cgtctgttcc tacgatcgat aggtcatctg gaatatgata gccattttct
    18901 gtcaacttat ctagcattaa actagctatt tgatcgttat agcaaatgat gccatctaaa
    18961 gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg tgtttccgtg
    19021 gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat actgttcaca cgcgctgata
    19081 aaacctttca tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa caacagacgc
    19141 tgatgtcctt tttttatcaa atattctgct gccataaaac cgactttcac atcatctaaa
    19201 caaatataag aggcatttaa ctcttcataa gcagcattga tcatcaccat cggaatgcct
    19261 tgttctctca aagcaacata aagtgctaag tttgggttat actgattact tttcgtaggc
    19321 tcaacgatca aaccatctac cccataagca atcattttct tcaaacactc tttctccaat
    19381 tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc cagattctcg taattcattt
    19441 tcgattcctc ggataatcga cggaaaaata taatcagaga gatatgtggt gatgacaccg
    19501 atcgtttttg ttctgttcaa tggtgttttt tctttcttat agctgtcatc tacatatgtg
    19561 cccgaccctt tttctttacg cagatagccc tcatttacca acacagcgat tgcttgccga
    19621 atcgtatgtc tgctgacgtc atatatttct tgcagcttga attctgaggg aatggttgta
    19681 tttacttcgt attcctcacg aaggatcttt tgttttatct cttctgcaat atcttgatat
    19741 tttgccattt gccttgcccc tttttatttg tacgtacata tatcataacg agtctgcaag
    19801 gtttattcaa gcaaaaatca aggtttcttg tgctcataag gcatacggaa caggttcacc
    19861 aaattctgga aatccttgct cattccacgt aaagcattgc gcgttcgcgt gccgattagg
    19921 attgtctaac ggatcccctt tttggttctt ctctggtcga gcatgataga tcaaaatatc
    19981 aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt gcttattttt
    20041 ttcagaagaa acgaatactg gctccgataa tttatgccat gattctttta ataaaagatt
    20101 cttgtgctca tcagcccaca gcaggcccat ggcatagttt tcatctgttg cactaccaga
    20161 atacgtaatg aatattttcc cattgcgagt aaggactgct ggaccttcat ttactgaaaa
    20221 accccgtttt tcccattcat actcaggtat agaaaggagg atctgcttcc cctttaacgt
    20281 ccaaggatta tccatttcgg atagataaag attagaattc ccagtaattt caggatcttg
    20341 ttgggcccac acataatata gctttccttg agaagagaaa gttgttccgt ctaaactgaa
    20401 agtttccctt ttcgttttta tttgtccctt ttctatccaa tctgtattca ttggatctat
    20461 atgctgattt tcaagtacga acatgcggtg atgatgagat tcatcacgta tagttttatg
    20521 attgcttgca gcaaagtaaa tataccattt tccattgata taatggactt caggtgccca
    20581 gatcaattca ctcatcggtc cttcttcatg agcctgccat acaatcaggc ttgcagcatg
    20641 ttctaattca ttcagtgact ttgcccttct taattcaatc acttggtagc ctggaaccga
    20701 tcctgtaaaa taataataac catctgtatg tttatatacc caaggatctg cccgttgccg
    20761 aacgatagga ttattataaa gtgtcttcat cataacaaat ttcctcacat tttctttatt
    20821 ctaattacat tccaagagta tttttctatc cctacattta attcgtcatg tttttctatt
    20881 gaaaaatagt ttatttcagg ttttattttt tctggttctt cacgtgtatt tttgtccaac
    20941 agatcgctac tggttaatgt gtagtgctct ccttgatcta gcaaattgaa atcatgttca
    21001 atcgttatct tttgttgttc atttgtgcga ttgatcaaga aaatagtcag ctgattgccg
    21061 ttatctacga tcacactgtc cacataagga acatttttta tcttttcaca atcataaaca
    21121 gggcaaatga tttgttgttt caatacttga cctttagcat atttcgataa gtgttgcatc
    21181 acataataag ttggattcct ccatgcttgt cctttagcat ctgtcagtat taaaggtatt
    21241 gtgtttacta acaatgattg acaactaatc tttacgcgat ctgcgtgtcg taaaatagcc
    21301 agccctaatg aaccaaataa taatgtatct tccatagtaa agtaacacca atcgatggga
    21361 ctaccgactt caaaatccct atgtcttttt tccggttggg catgtacatt ccattcatca
    21421 aacgctagat acattgtttt ctcacttctc aaatatcctt ttacataatc acaagtagca
    21481 acgatttctt caatttgacg atcgaatcga taagcttttg caaggtatgt cgctgtatca
    21541 tcacgctcat ttttaggagg tttagtcaag tcttcttcat catacttatc aatatagtta
    21601 tgcaaagcta aataatctac attctcataa gcttcttcta atgcgatacg atcccattca
    21661 gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat tgaatcatcc
    21721 actaacttca tgacttttga ggcttcattt gctaacctac catattcata agcactctta
    21781 gcccccactt gccatggacc gtctagctca ttccctaaac accataattt aacgccaaaa
    21841 ggcttttttt gcccattttt tattcttaaa tcactccaga aggttccttt tggaaaatta
    21901 caatattcta ctaaattttt tgcttcatca attccttttg ttcctaaatt caccgtgaat
    21961 atcggtttcg cttgtacatg actgatccat ttaaaaaatt cgtggagtcc aaaagaattt
    22021 ggttctatac ctcgccatgc taaatcaatt ttttttggtc gttgttcttt cctacctaca
    22081 gtatccaccc aatcataacc agatgtaaag tttcctccag gatagcgaat gacacctaat
    22141 tctaattcct ttacataaga gaggacatct tgtctaaacc cgtcaccatc agctgtttca
    22201 tggcccggtt cataaatccc tgtatagacg acacttccca tatgctcaac aaatgagcca
    22261 aacagccgag aactgatctc tcctaccgta aaatcctctc tgatttttac tttcacttct
    22321 tcgtccaaca gctttcccta cctttcattt cctatagtca agataataga tacaagtttc
    22381 catacgccac cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag cagtacaaat
    22441 cctaataaca atacgcaaca aagaatattt ctcaaaaaat caaaaaatac tttccatgca
    22501 ctttgttcaa aaatttgtaa aaatgtgata
//
LOCUS       DAZKOK010000038        22117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000038 DAZKOK010000000
VERSION     DAZKOK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.038"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..1500)
                     /locus_tag="ACG90W_001285"
     CDS             complement(127..1500)
                     /locus_tag="ACG90W_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0477660.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1500..1895)
                     /gene="rbsD"
                     /locus_tag="ACG90W_001286"
     CDS             complement(1500..1895)
                     /gene="rbsD"
                     /locus_tag="ACG90W_001286"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0477661.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1923..2840)
                     /gene="rbsK"
                     /locus_tag="ACG90W_001287"
     CDS             complement(1923..2840)
                     /gene="rbsK"
                     /locus_tag="ACG90W_001287"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0477662.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2948..3961)
                     /locus_tag="ACG90W_001288"
     CDS             complement(2948..3961)
                     /locus_tag="ACG90W_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0477663.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(4042..4641)
                     /locus_tag="ACG90W_001289"
     CDS             complement(4042..4641)
                     /locus_tag="ACG90W_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0477664.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4827..5528
                     /locus_tag="ACG90W_001290"
     CDS             4827..5528
                     /locus_tag="ACG90W_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477665.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5538..8249
                     /locus_tag="ACG90W_001291"
     CDS             5538..8249
                     /locus_tag="ACG90W_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0477666.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8700..10328
                     /gene="treC"
                     /locus_tag="ACG90W_001292"
     CDS             8700..10328
                     /gene="treC"
                     /locus_tag="ACG90W_001292"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0477667.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10388..11569)
                     /locus_tag="ACG90W_001293"
     CDS             complement(10388..11569)
                     /locus_tag="ACG90W_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0477668.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            11821..13344
                     /locus_tag="ACG90W_001294"
     CDS             11821..13344
                     /locus_tag="ACG90W_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0477669.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(13413..14141)
                     /locus_tag="ACG90W_001295"
     CDS             complement(13413..14141)
                     /locus_tag="ACG90W_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0477670.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(14154..15434)
                     /locus_tag="ACG90W_001296"
     CDS             complement(14154..15434)
                     /locus_tag="ACG90W_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0477671.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15575..16177)
                     /locus_tag="ACG90W_001297"
     CDS             complement(15575..16177)
                     /locus_tag="ACG90W_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0477672.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            16422..16628
                     /locus_tag="ACG90W_001298"
     CDS             16422..16628
                     /locus_tag="ACG90W_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477673.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            16940..17659
                     /locus_tag="ACG90W_001299"
     CDS             16940..17659
                     /locus_tag="ACG90W_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0477674.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(17740..18423)
                     /locus_tag="ACG90W_001300"
     CDS             complement(17740..18423)
                     /locus_tag="ACG90W_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0477675.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            18641..20827
                     /locus_tag="ACG90W_001301"
     CDS             18641..20827
                     /locus_tag="ACG90W_001301"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0477676.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            21173..21583
                     /locus_tag="ACG90W_001302"
     CDS             21173..21583
                     /locus_tag="ACG90W_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0477677.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            21597..21953
                     /locus_tag="ACG90W_001303"
     CDS             21597..21953
                     /locus_tag="ACG90W_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0477678.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         7570 a         3921 c         4295 g         6331 t
ORIGIN      
        1 ggttatgcat atgtaaatta taccaaagtt tggaaagtat cacaaaaatt tataaaaata
       61 atgatcctaa aaagaaaaag actgaaatat gtgataaaca tctcatactt cagtcttttt
      121 cattaactat tcataagaca ttaggtcttc atcaaacccg ctaacgagag tagtgatcat
      181 tgctacgact agactaacag ccagtcctgc taagtaccag aaaaaagtac cggaaatgaa
      241 tgcaggcaat gtggtcagac taccaaatac aaaagcgatc gctttcactt gggcaaatcc
      301 cattacagct ccaccagcag ctgcaccaat gcaagcagca acaaatggtt ttttcaatgg
      361 gatcgcaatt ccgtaaacag ttggttcggt cactcccgca agtaaaatcg aagcaaaagc
      421 agaaccggag atcgaacggg tttttgaacg tttacttcgc agaaatacac ctaaagctgc
      481 accagccatt ccaaaattgg ctgctcctgt caacggaacg attcggtcga acccataagc
      541 agagatatta ctgatcatga cagggtttac agcccattgg ataccaaata ttaccaaaat
      601 attccaacca ccagcgatga ttgcgccact gatcggtccg ttaatatcca tcatccaagt
      661 aattgcggca gctaaacctt cgccaaggta aacacccgca ggcccgaata ccattaacgt
      721 caaaggaacc gtgataacta atgaaagtaa aggtacaaat actaattgca tattttcgcg
      781 aatatatttt ttgaggaatt tttcaacaaa agaataaaag aaaataccta aaatcgccgg
      841 catgacagta gacgtatact gcatcagtac tacaggaatc cccagaaaat cagtagctgc
      901 tttgccactg tctgaaagta aagaggtcag attcggttca atcagtgctg cagcaatgac
      961 agccgcaata tatccattca ctttaaacgt tttggcagca gaaaaagcaa gaaagattgg
     1021 cagaaaataa aagacggcat tcgacgcagc agacaaaata tgataagttc cgcttgtttc
     1081 actaagccag ccaatcgtag tcgctaaaac aaccagacct tttaatatac cagaaccggc
     1141 aaacagcgga atgatcggcg taaagatttc cgatatcaaa ccaaatagtt tttcgatcgg
     1201 atttagcttt tctttcggag ctgaatgatc gatttgacgc gtcaattcct tgtaagcagg
     1261 agccaatccc tgatccatga ttatttctgc atatctatcg agtaaaatga aatacttaat
     1321 ggaagggatt tgctgtttta atttttctgc attggctact ggctgttttt tcaatttgat
     1381 acgaagccga gtcgcacatg gaaggacaga atcaatattg tcttttcctc ccaactgttc
     1441 aatcagcata tgaatctgct cggacagtac catctgttca ggattcattg tcgtttccat
     1501 tagaagacca ctccactcac taatataata ttggcgtaag gtgtcatttc gcctgtacga
     1561 ataaatgcat gacagttatt caactctttt ttcatttctt catgtgggat aaagctaatc
     1621 ggtgtatctg gtaacaataa tttgattttt tccaatattt ccgggttcat tgtctttatt
     1681 tcttccgcca attcaatcga ttgtacctca agttcttcta aaacgttttc taaaacagat
     1741 aaaaaacttg gaagaccgtt tgagacagcc aaatctattt tttcagtacc aaaggggaca
     1801 ggcatgcctg catccccgat acttaattta tcaaagtgcc ccatttgtgc gattactcgt
     1861 gaaatatctg aattgattac ttttcctttt ttcatgtttg tttcttcctc tcattgttaa
     1921 ttttagactg ctgattgttc taaagcttcg cggtaaggga tagaaggttg cgcaccataa
     1981 gactgcacag caacagaaga ggctagattt ccataaagga tcgcttcttt caagttacta
     2041 aggtctggct gaagtgtgct gcttaatgcg ccaataaatg tatcacctgc ggctgttgtg
     2101 tcaacggcat ccactttaaa agcagggacg ataccgtgtt ctttttctgt atgatagaaa
     2161 gcgccggcac tgcctagtgt aatgattact gttccaatac ctaattcatg gagtttttcg
     2221 gctgctgcga ccaaactgtt atgatctgtc actctgattc ctgtaatgat ttctgtttct
     2281 gtttcatttg gtataatgat atctgtcttt tcaagtagtt cagtaggaat cgtttcacga
     2341 gctggtgcag gattcaggat cgttgttttt cctgcgtctt ttgcaataga aaaagctagg
     2401 atcgtcgcat ctaagctact ttcaaactga gcaataataa agtcactgtt tgtaatcagt
     2461 tgcttgtttt ttaggacttc ttgttcatgg aaagcattat ttgcccctgc atggatcaag
     2521 atactatttt ctcctgctgc gtctaccata atcattgctt gtccggttgt ggcgccttgg
     2581 atttcagcta tcccagatgt atcgatgttt tcttttgtca gtagatctaa taactgttgt
     2641 ccttcgctgt cagccccaac gcctccaata aaagatgtcc gtgcgcctga acgtttggct
     2701 gccactgctt ggttggcacc tttacctccg ccagcgtgaa aaatctcatg cgcatgcatc
     2761 gtttcgcctg gcttgggcat gttggttaaa cgaagggtag tgtccatatt aatacttcca
     2821 attactgtaa ccgtgttcat tttacaaacc tccaactatc tcatttattt tgatgggaaa
     2881 attatttttt aaaacgtttt agtaaaacgt tttaatagtt cgataaaaat atagcacacc
     2941 ttgaaagcta tttcaagggt gcagttgaaa atcttttttc taatcgtaca ggtaatcttt
     3001 tttcttccca ttctttttct gggaattgga tccgatctaa caataacttg gcagatgttt
     3061 gtcccaattc gaagattggt tgtgcgatcg ttgtcagttt cggcttgata tactcgcaca
     3121 tatctatatt atcatagcca ataatgctgt agtcttcagg gatgcttttt ccagcttcct
     3181 ctaatccgcg atataatcca aaagctaatt catcatttaa agcaaacaca ccagttgcag
     3241 cagaatcaag aagttctgct gtgatctggt agccaccttg ttttgagaac tgtgtaggta
     3301 agaggatcag ttggtcgtga gggatttggt aaacatcaag agcagattta aacccctcaa
     3361 tccgagcgtg gacattttcc ggtggatttt caggatagac aagggcgatc gtttgatgtc
     3421 ctagactaag caagtgcatg cccgctaggt aaccaccgcg aaaatcgtct gttcgtacgg
     3481 catcactaaa tccttcagat tttttctgat ccaaaacaat gaagggccgg ccttgttttt
     3541 tcaaattttc gttgattgca tctgtagaga cagcagaagt cgcgataatg aatccatcca
     3601 cacctcgacg tgtgagctcg gctagatatt cgatttcttt ttgatgatca gaatcagcat
     3661 tacataatat cgtgacgaaa ttctgcttat acaagatatc ctcaattcct cgcatcagtg
     3721 tactgaagaa tggattcgtg atatcaggaa cgagtacacc aattgttttc gtttccttgg
     3781 taatcatttg tctagcaaaa tagtcaggct gatagcctaa ttcttctttg gcagctaaaa
     3841 ctttctgaac agttttcgga ctgaatttct ccgtatttcc atttaatatc aaggagacag
     3901 ttgtgattga aacgccggaa agtttagcga cttctttgat tgtgatcttt tttttcacca
     3961 tgttcatact cctatctaaa tcctgtggtt attttcccat gaaatcaaaa agaagtaaat
     4021 gaaaatcacg tgtgccagta cttatttgct ttgttggata gcttcgatta tttgtttcgt
     4081 tgagcgtact cgtgcagttt tcggaaatat cttttttatg aacaattgat gggattcttt
     4141 gtcacgatca ctgcaagcat cttcagcaag cacgatatgg tacccgtgtt gaaaggcatc
     4201 taatgcagta gcatatacac cgtttgttgt cgctacaccg cttaaaatga tagtttcgat
     4261 tccacgccgg cgaagttgaa gatcaagaga agtaccaaaa aaagcacttg gattgtattt
     4321 tgtgatagtc aacatattat ctgtatcttt caaagaatcc ttcaaaagta aatcagtgaa
     4381 ttctggaggt acagaagcag tcattccacc catatcagtt tgttgactca aataagggaa
     4441 agtggagatg tctacgttca ctaaaacttt caaggcagcg gtacgtttca atgttcttac
     4501 taacaaatca ttgtttgtca gaatagatgc tgcagtgtgt ggagagagtg taccgttatt
     4561 tacgatctct ttttgcatgt cgatcgcgac aaaagcagtt tttgataaat caaaaatcat
     4621 attttccttt aaatcattca tgatgttcct tctttcaaat aacaatgttt tttatgtaag
     4681 taaatcataa atcgtttatt tgtcaactaa attaaaggat tcctttcttt agtctagaga
     4741 aattcagtgg taaaataaat aagtgtgaga aaacgcattc gatgcataga tatagcgaga
     4801 aatatcgtga aaatggagga atcagagtga agtatgtcga agtaaaaaat gaatacaaac
     4861 gatacaaaat gggagaaacg accattacag caaatgacga tatttctttt tcaattgaaa
     4921 aaggcgaatt agtgattata cttggtccaa gtggagcagg aaagtcaacg attcttaata
     4981 ttttaggtgg gatggataca cctgatgaag gtcagatcat tattgatgac acagatattg
     5041 cgcaattttc agataaacag cttactgctt accgacgaac agatgtaggg ttcgttttcc
     5101 aattctacaa tctcgttcct aatctaacag caaaagaaaa tgtggaattg gcaacggaag
     5161 tttctcctga tgcgcttgat cctgtagagg tattgaggca agtgggccta gctcatcgat
     5221 tgaataactt tccgtcccaa ttatcaggag gcgaacagca gagggtttca attgctcgtg
     5281 cactagctaa aaatcccaaa cttcttttat gtgatgaacc gacaggcgcg ctggattttg
     5341 aaacgggcaa gcaagtattg aaattattgc aaaacgctag cagacaacac ggcaataccg
     5401 ttttgatcat tacacataat tctgctttag cgcccatcgc tgatagagtc attcacatta
     5461 atgatgctaa agtaaggtct gttgaattaa atgatcatcc ttcatccatt gatgagattg
     5521 tatggtaggg ggattttatg aaaacaagaa catatcttaa agcaggtttt cgcgaaattc
     5581 ggcaatctaa aggacgattc attgcaatca tccttattgt catgctagga acacttttat
     5641 ttgtaggtgt caaaactgct ggacctgtca tgcaaaaaac gatggatcac tatgttggta
     5701 cagcaggact ttctgatttg caaatcgttt ctactggtgg cttgacacaa aaagatattg
     5761 ctcaagcaga aaagatccca gatgcaaaag tagaaacagg aaaacagatc tattattcca
     5821 atactggcaa aaacgaagtg attcaaatat tttcttataa caaaaataac aagcagaaca
     5881 agctacaatt aactgatgga aaactcccgg aaaaaccaaa ccagcttgta ttggatgaaa
     5941 aagcggaaga tgaagggtat aaaatcggtg atatttatca gattgattca gatgaattga
     6001 aagaaaaaga gtataccatc acgggctttg tccgttctcc attgttcatc aacaatttgg
     6061 agagaggata cgcgaacgtc ggaaatggta gcgtcgatta ttttgtctat ttacctgaat
     6121 ccgtttttaa aacagatatt tattctgtct tatatcttga ttttacaaac gtaaagaatt
     6181 tgaatacgta cagcaaagct tacaaagata aaatggagca aaatcaagaa aaggcagaaa
     6241 agtatttaca agatcgaccc gaagaacgct tagaagagct gaaaaaatcg gccaacgaag
     6301 aattagaacc ggcacagcaa aaaatagcgg atggcaaagc acaaacaacg caagcaagaa
     6361 cacggttaga agatgctaaa aaacagttag cgcaacagtc agcaactatc cagcagcttc
     6421 ctgaagcgca acgaacaatc gctgttcaaa cattatctca acaagaagct caacttgctg
     6481 aacaagaaag gcaattagtt gagaaagaac gtgaactggc ctctgcagag gaagaattga
     6541 atcgaaatgc agaaaaaatt gaaaatttaa ctaaaccatc atacctatac aatgaacgtt
     6601 cagaaaatgt cggttttcaa gaatttggtg atctgtctga tcgtatcgct gcgattgcca
     6661 atgtttttcc tgttttcttt ttctttattg ccgcattgat cacctttacg acaatgacac
     6721 gtatggtaga agaaaaccgc agggaaatcg ggacattaaa ggcgttaggt tatacgaaag
     6781 ttgaaatcgc aaaaaaatat gcaatctatg caagtctagc ctcagggata ggtattgttt
     6841 taggaacgat attaggaacg aatcttttgc caaggatcat ttttgagctg tctaacgagc
     6901 ggtatgatat tgggagtgcg cttatttttt atgactggcc gccgatttta caagcagctg
     6961 ccgcattctt cattgccgca tttggcgctg caatgatcgt tttattcaaa gatttaagag
     7021 aacggcctgc tgcattgctt caaccgaaag caccgaagcc agggaaacgc atttttcttg
     7081 aatacatcac tccgctgtgg tcacgcctta gctttaatca aaaaatcagt taccgaaatc
     7141 tcttccgata taagtcaaga atgcttatgg cgattatcgg aattgctgga tgtgccgggc
     7201 tgatggttgc aggcgtggga ttgaaagatt ctctaagttc tgtttcagcc aaacaatttg
     7261 gaccgatcat tgactatcaa gcaatcgtta cattggattc atccacggaa gatagtcgtc
     7321 aaaaagcagc ggatatgctg cagaagcaag aaaaagtaaa tgagatccta gctattcata
     7381 ctcaaaccgt agaattgaga aaaaaaggac atgcttcgca aagtctgaca gtgatcgtac
     7441 caagtaaaac agctgatttg gatcgtttta tccatttaac tgatcttgaa gagaacccac
     7501 gctccttgcc taaaacagga atcgctatta cgcaaaaagt agcagaacta tttgacttat
     7561 ctacaggaga tcatatttcg ctttatgatg aagatcaaaa agaatggaaa gtcgaagtaa
     7621 aagaaatcgt gaaaaactat ctaggtagct ttatttatat gtcccctgaa tattatgctt
     7681 cagttactga tcaatcgatg atggataatg catttttgct gcgtacagat aagatgacaa
     7741 caaaagaaga agaaatgctt tccgagaaac tgcttgccac agacgctgtg acaaatacgt
     7801 ctttcatgtc caaacaaatc gaaacgcaag aagaatcaat ggcgaactta gactcggttg
     7861 tcttaatctt tgtcgtatta tcaggactgc tggcttttat cgtcttgtat aatctaacta
     7921 atatcaatat ttccgagcgt gtacgcgaat tatctacaat aaaagtcctt ggattttttg
     7981 ataaagaagt cacgatgtac attgttcgag aaaatatcat ctttactctt ttaggaatca
     8041 tcggaggatt tggtgtagga tatatattga ctgacttcat cttgcaacaa gcgtctatgg
     8101 agaatgtcat ctttccatta gtgatcacat ggtgggctta tgtcttatca gcaggattaa
     8161 ctatcgtatt tacggttatt gtgatgattg ttacccattt caaattaaag catatcgata
     8221 tgattgatgc gttgaaatcg aatgaataaa aacagaggtt gtgaaatcag cggtttgacc
     8281 tgagtattag agaaacaaac ggcaaaatag tttttcatat tttgccgttt tttgagctaa
     8341 taccgaagga cagctgattg aacaccatga atcagaggtt gtaaaaaagc cgtcctgcat
     8401 cgagcaaaaa gaatactcaa ataaaattag ctcctcatgt tttacatttg agaagactga
     8461 ttgccgtaga taaaagcttt tgaacaccat ttatagaaga aacaagaata ttttgctatt
     8521 ttaaacaaga ggatagacaa atacatgtct gatctcttgt tttttattgg gcaacagacc
     8581 tcttagaatg tataaagata aattttacaa acgctttcgg ttcagttata ataataaatg
     8641 ataacttgta tatataagtt gtggtaggta ataaatcagt tgaatggagg taaatatcca
     8701 tgacatttaa agacaaagtc atttaccaaa tctatcccaa atcttacaaa gacagtaatg
     8761 gtgatggaat tggagattta aaaggaatca aagaaaaact cccgtatctg aatgatctag
     8821 gcatcgacat gatttggcta aaccctattt atccaagccc tcaaaaagac aacggttatg
     8881 atatctctga ttacacagcc atcgatccta tctttggtac gatggaggaa ttcgaacagc
     8941 taatagccga agcaaagaaa taccggatag aaatcatgct cgatatggtg ctgaatcacg
     9001 tttcgacaga acatgaatgg ttcaaaaaag cactagcagg ggaccagcat tatcaggact
     9061 tcttcatttt aagagaagag ccaacggatt gggtatcaaa atttggtgga agtgcttggt
     9121 cgccatttgg agatacgggg aaatattatt tgcacttata tgatcggaca caagctgact
     9181 tgaactggcg gaaccaagca gtaagagagg aactattcaa agttgttcgt ttttggatgg
     9241 aaaaaggggt aaaaggattc cgtttcgatg ttatcaatgt tatcggtaaa gacgaacaat
     9301 tgaaaaataa taaagaaaat gatggcaaac ctgaatatac ggacaaacca atcacccata
     9361 cgtatctaaa agaattgaac caagcaacgt ttgggacgga ttccactatc atcacagtcg
     9421 gtgaaatgag ctctacgacg atcgaaaatt gtatcttgta tacagaacct agtagagaag
     9481 aattatcaat ggtctttaat ttccatcatt tgaaagtcga ttatgaaaat gggaataaat
     9541 ggtctactcc tccatttgat tttgcagaat taaagcgact atttcataca tggggagaaa
     9601 agatgagtga gggtaacggc tggaatgctc tattttttaa taatcatgat cagccacgtg
     9661 cgctcaatcg atttgtagct atcgatcagt atcgaaaaca aggagcagag atgctagcaa
     9721 ccatgatcca tttgaaccgt gggacgccct atatttatat gggagaagaa atcggcatga
     9781 tcgacccaga ctattcatcg atttccgact atattgacgt agaaagtttg aatgcttata
     9841 agttactcct tgaacaagga ttcacagaaa aagaagcgtt tcgtcggatc aaagcaaaat
     9901 ctcgcgataa ctctcgaaca cccatgcaat ggtcagacac agaacaagca ggatttacta
     9961 cagggaaacc ttggttaaaa gttggaggca agctagaaga gattaatgta gaaaaagaac
    10021 tatcttctga agattctatt ttgtcctatt acaagaaatt gattcggctg agaaaaacat
    10081 atccaatcgt tgcacaagga gattatcatg cttatgaagc aaatcatccg gaagtttatg
    10141 gctttcttcg ccaatttgaa ggacagcagc tgcttgtatt aacaaatttc tatgataaag
    10201 atacaaggat cgccatccca gaaggattca cagaagccaa tattcttatc gataattatt
    10261 ccgatactgt tcttcatcaa agtctggagc taaaaccata ccaggcaatt gctttgctga
    10321 aaaattaaaa caaaaacaca aaacgtcgtt cctacgagct attctcgtaa gatcgacgtt
    10381 ttgtgtttta tttaaaataa tcttcttcag aaaatgaaga gttttcatac tctcctttat
    10441 cgccataaaa tttattgtag cgttgtttga atttacggaa aataaaagta acgagtaatt
    10501 tgactaacgt atagatagga ataccaaaaa taacgcccac aattcccatt agatcaccca
    10561 tgacaagtaa aacgattaag atcgtaatcg gatggatctg taaacgatca cccattactc
    10621 gaggaatcac caagtcaccg tggagaagtt gtacggcaaa ccagacaatg acaaatttga
    10681 cagccataaa agtagagtct tggaatgcaa tgatcaaacc tggaaagaag gcgataaacg
    10741 gcccaatata tggaatgata cataacaacc cagcagaaat agctaaaaca ctagcaaatt
    10801 ctaatccgat cagtaagaaa caaacccagt agactgctcc aaggataaat gaagcaatga
    10861 tttgcccctt tagaaatgaa cccaattgtt gattagcaat ctttgtcaaa tttttaaaat
    10921 ctgtacgaaa tgctggtggg acaatcgcta aaactgactg ataaaatttt tgaggatctc
    10981 tcaaaaggaa aaaagcgaca attggaccag taaataaagt aataaaaata gctgtaacag
    11041 ttgaaaaaat gttgctgatc cgttgtgcgc cactttccca ataatttcca ataaatccag
    11101 ctatatcaga cgtcacgtcg tcaatagaag cgaaaaattg attaaatgaa tcggcaaaag
    11161 gtgttttctc caaaaattgt cggagactct cttgaaaatc ttgaaacaaa tctggcaaac
    11221 tactgatcaa tgtctttgcc tgttcttcaa gaaaaggaat aagccatatg cttccaagcc
    11281 ccaataaaaa taaaataatc acataaacga taatgactga caagttcctc gaaaggtgct
    11341 tgctcaaacg tttgatgact ggttccaaaa gataatacaa gatcagtgca aacaataatg
    11401 ggggcaagat agtagcgata atgattccga caggcttgaa tataaacgag atctgatgga
    11461 aaaaataaat gatcagcgca agtaagataa ctattcctaa aacatagtaa gaggtttttc
    11521 ctcctaaaaa gcggatcaaa cgattcgttg tatctgtttc ttttttcact ctttttcctc
    11581 ctttatctat aaattcatta tagacaaaag cgggtataaa aggaaaatga tagaggttga
    11641 ttgcaaaaat atcgtcaatc attcatataa actttctcat caattaaaat aagaaaacgc
    11701 ttgacagttc ttttaaccat atgataaatt aaatcctgtt gctaaaacgt aactattacg
    11761 gtttgcgaaa agcgaatcgg aacattcaaa tgaaagaaga tgagaagagg agtgtcataa
    11821 atgaaacgat tgataggtat attggccatg ctggttttag caggtatgtt aactgcttgc
    11881 ggggcaagtg ggaaagccga ggacagtaaa gaaaaattga gcgtgatgac gaccttttat
    11941 cccatgtatg attttacaaa agcaatcgtc ggggatgaag gagaagtaga gttattgatt
    12001 ccagcaggga ctgattccca cgattatgaa ccttctgcta aagacatggc aaagatccaa
    12061 gatacagata tctttgttta taatgatgaa aacatggaaa catgggtccc cgcaatccaa
    12121 aaaacattac aagaaggcaa tgtccatacg ataaaagcga cagaaggtat gcttttactt
    12181 ccgggaagtg aagaaggaca tgatcatgac catgaacacg gggaagaagg ccatacacat
    12241 gagctagacc cacatgtatg gctagctcca agtttagcaa tcaagcaggt agcaaacata
    12301 cgtgatcagc tgattgaagc ttacccagaa aaacaggaag tatggacaaa aaatgcagca
    12361 gcctataccg agaaacttca agcactccat cagctttatc aagaaacctt taagcaagca
    12421 aaacaaagat cttttgtcac gcaacatgct gcattcaact atctagcgtt agaatatgga
    12481 ttgaatcaag tatcgattgc tggtctttct tctagtgaag aaccgtctgc ggcgagaatt
    12541 gctgaactga aacactttgt taaagaacat ggaatcaact atatttattt tgaagaaaac
    12601 gccaaagata gtattgccag aacgttagca aacgaagcag gtgtatcttt agaagtattg
    12661 aatcctttgg aaggattgac gaatgaacaa atagaaaatg gagaaaacta tctttctatc
    12721 atggaagcaa acctcgaagc gttgaaaaaa acgacagaga cagaaaaccc gctagaagat
    12781 actttgaccc ccaaaaaaga aaagagcgtc tacaacggtt attttgaaga cgaggatgta
    12841 caagatcgct ctctttcaga ttgggcagga acttggcaaa cagtagatac gtatgtagaa
    12901 gatgggactt ttgatcctgt ttttgaatat aaagaaaaat tgaatcagga taagacagca
    12961 caagaatata aagaatatta tacgaagggc tatcaaacag atattgcttc aatcaagatc
    13021 agtgagaaag agatggcatt tacgtttaag aatggaacaa cgaaacgttc aaattatcgt
    13081 tatgtaggga aagaaatctt gacctacgag gcaggaaacc gtggcgtaag gtatctattc
    13141 gaagcagaag ataaggaaag tggcgctttt agatatattc aattcagtga tcacacgatt
    13201 gcaccggctg actccgaaca ttttcattta tatgcaggaa atgaaagtca agaagtattg
    13261 ctgaaagaaa tggagaattg gccaacattt tatccagata caatggatgg acgtgaaatt
    13321 gcacaggaaa tgcttttgca ctgataggca aaaaagaacc gaagggtttg gatcggctgg
    13381 tcatttagtc ggtccaaact attcggttct ttttatgaaa tcaatttctt taatccacgg
    13441 atagaggctg ggatggtctc gccgtttgcc aacgtgactt ctctggtttt tttattaatc
    13501 gaccgcacat tttgtgtatt gataacatag gatttatgac aacggataaa attccgatgc
    13561 attttctcga tttctttgat cttctcatag aattgtaatt gggcatgagt caaatggact
    13621 tccagtttat gtggcgctcc tagcgaagta gaaaaatatt ggatgtcttt gatcggtaaa
    13681 aatttgatct catcattaaa ctctattttg aagatatctt tttggatttg ggacatttga
    13741 aaatcttcga aaattttgct caggatatgc tcgacatttt cttttaaatc ataaagatct
    13801 tctttgacga tataatcagt cggttcgatg ttgcttttta agattttcat cgacatttcc
    13861 gtatgggaag tcacatagat tatttttcct aaaggatcta attcacggat caatttaccg
    13921 atcgctacac catccatttc actgtcttct agttcgatat ctaaaaagta aataccaaaa
    13981 gaagaagttc tgatcttggc tgcattgaga agttcttccg gatcagaggt agaaatatgg
    14041 acataactgt ctaagttttc aatcatgatt ctatttttga tcaccgttga taaaacttct
    14101 aattgttttg gatcgtcttc acaaatataa atgggaatca tatcattact cctttatttt
    14161 atttgtatat aaatcgtttg agaaaaacgc tggttttcaa ttgatgtttc aaggaaaaga
    14221 taaggattgg tctttgtcaa ttcatccaag ataaacaagc caaggccttc gttttttgga
    14281 tttgtttttg ttgagaaccc tttttgtttc aactggttta acggtaatgt atttgttgtt
    14341 gtattgatca cacgaatcat cacggaatga ttcttcttga gtatggtcaa ttcgattttt
    14401 ttcttctttg cagcactagc tccttcaatc gcattgtcca agataatacc aagcatacgt
    14461 acaaggttta ccgtatgttt cgtatccagt tcgattggct caggaaaatg aagctggacg
    14521 atcaaccctt tttcctgtgc catcatgagt tttaatgaca gtaaactacg gatctcagga
    14581 actttcatat tatccaacat acttagattg gccggaaaaa ggttcatcat ctggcgtgta
    14641 ggtaatatcg tttgatcata ataagaccgt aatccttcta agtcgccttc tgaaatatag
    14701 ctgtgaagag aaaaaagcag attttggtaa tcatgtttga attctctcaa gtcttgatag
    14761 tttttttcta gatctttgat ataatcctgt tgcatcctga cttgttgctc taataactga
    14821 tcagctaatt tcttattaga gattttccaa taataaacca agatgatcag gccaacacac
    14881 agatatagaa tgaaaaaact cgtattcaaa gctaagacat caggtgtttc accagaaaaa
    14941 cgggtataaa aaataatgat atagtatgta aataatatca atgtcccgat cgtaccgatc
    15001 atacggcggt ccacagtgtg aaaccaagtc tctaacgatt tttttaaaca aaaagctaat
    15061 aaagcagata aaaaagcgga taagacaatg tgaaaatact gcaatgaatc tcctggttca
    15121 ataaccgaag atttcaaaac aaaaaaatcg ccgatgcttg ttaagtgatc tcctaaaagc
    15181 gacgtcaata acccaaaagg aagcgcgtaa accaattgtt gacgcgaagt cgaaaagaag
    15241 aaaaaagtaa gcatcagcat tcctatagca attagagcag taaaaatccc taaaatcgaa
    15301 tagacccatc caatcaaaat acaagaaatc agaatgactc ccgcaaacag atatttgtat
    15361 cggatgtgat ggtcaatcaa taagatacaa attgtgtaaa tcaaatattg caacgctatg
    15421 gaccataaaa acataaagta cagctcccat tgatttttac caaattatag cacaattggc
    15481 gtattatttt tttaattttt cagggatttt gaattcagaa ctcataccaa agcagcagcc
    15541 agatacaaaa aagtcagata agttagttaa ttgtttaatg attgtttttt tcatgaaaga
    15601 ttcctccaaa attcatcaag tagtttgtaa taggaagcaa cgtagctatt tctaaaacaa
    15661 tcccaagtag caaataggta gaaaagtaag aagctgagaa aaataacgta atgagagaaa
    15721 aaattagaaa catgctgatc gccttcttta ataatggggg acgtttctcg ggttcaatgg
    15781 gattgattgc tgtacctctt gatccatagc gaagcagtaa caaaaagcag ctgattaaca
    15841 aaaagaccat cattgggaag gagatagtca attgcagatg tttcatcacc aagggaacgc
    15901 cccataaata aagtagtgtc cacagcagac aaacgaattc agagtcggaa tgcaatccat
    15961 aagcatatct gcggatgcag atataagaaa gaaaggtgat tcctgtttcc gcccatgagc
    16021 ctgtcaccag agaaaagact agaacgaccg ctagttttcc aagattattc aataaaactt
    16081 ctaagacaaa ttttacttgg acatacacca tatcttcttc cgatttgtct ccgctgaata
    16141 aggcttctaa taatttgttc acaactttat ctttcatctc atacgtacct ccaccacaaa
    16201 ttttatgcta aaaaaaataa aaaacgtctt ttgtttcata aaaagtggtt ttgacatcaa
    16261 aaccgtcatg atctcctaaa aaaatcttcg ttttccaaga accttaccaa aaaaagtgac
    16321 agttttgata cgaaaagtac tttttatgac aagtttgtgc tttttcgagt gatttatgtt
    16381 aaaaaattac tgaccttata aaaggtgatg gagggattct tatggaaaat gaacaattgt
    16441 ttgaagtcgg cgaaacatat gcatttaaat ctgatttatt tgaatcacca ataaaaggaa
    16501 aaatagaaaa aatctatgaa aatagtgtga tgatacgtgt catgaaatgc gcagtagaag
    16561 acaatgatac cgctcataat ctgcactggc agttagttgt acgcaaatca agtacaatag
    16621 aagaataaaa acagataaaa tgttgagagc ataaagaggt tgtgagtctc ccgtttagct
    16681 ctgagttaca aggaataatg ttaaaaaaca gcttttcatg ttttatgaat attgccttgt
    16741 gaccgaggtg ttggcaggag aatactgttt atttgaaaaa agggctgcga caagactttt
    16801 gtcacagcct ttattttcat gaataggagt tacttttggc tacttttaga gaagtagctt
    16861 ttaaggagca gataaagtca aggcttttat ttctataaat gaagtagtaa aatgaagaac
    16921 agattttatt acaggaagga tgccgataat tgtgatcatt gttgcaggaa cgatcggtgc
    16981 agggaaaagc acactaactg aaatgcttgc ccaagattta gaaacaaaac ctttttatga
    17041 gaacgtggaa gataatgaag tattgccgct attttattct aatccagaaa agtatacttt
    17101 tttattacag attttctttt tgaacaaacg ctttttggca atcaaagatg ctttcagcca
    17161 tgatgacaac gtactagacc gctccatcta cgaagatagt atgctgtttc atttgaatgc
    17221 agatttaggg cgtgtttctg aagtagaagt aaagcagtat gaaggtcttt tagaaacgat
    17281 gctgaaggag ttagaagaaa tttcgccaca aaagaaacca gatctactgg tttatattcg
    17341 tgtatccttt gaaactatgt tagcgagaat caaaaaaaga ggccgggaat atgaacagct
    17401 agaacaagat cctgaactct attcctacta taaagaactc aatcgacgat acgaagagtg
    17461 gtatgaacag tttgatatat gtcccaaaat agtgatcgat ggggacaaat atgatttcgt
    17521 agccgatccg gcatgtggag aacaaatcgt acaagaaatc aagatgcggg caaagaaaat
    17581 gacagaagag gcagatgccc agtcgatccg ctacactaca aataacccga aagcaaaggg
    17641 aatctttaca actaaataat ataaaaacta agcaaattgc tcactcgcag atatttctct
    17701 gtgagcagca tcttgcttag tttttttgat tcattgagat caaatagtga accgattcgt
    17761 atgataaaat tgccataaat gaaatgtctg agtgagggcc atgatcaaaa ataaaatgga
    17821 aacgatcaaa atcgaatggg cgccaaacag atgatgacag aaaacaagga caatagctcc
    17881 tgcgataaag gcaagaacga cacttagata gtggaaaaat cgatcaaaat ctgctttttc
    17941 tttagaaata caataatcat ataagttcgt tgctacccct tttaaatttc cagttgtaaa
    18001 taaattagtg taggcacgcc cgttgatttt atcaaaagct acccactgga tcgatgcagt
    18061 aaaagcaatc aaaatcgtta ctaatgcggg atgctcatgg acgggtgtca aactaacaaa
    18121 gaaaaaaatc actgcttcat aatatagaac aaacaagcgc cagaaatgtt tttctttttt
    18181 tctaaaataa tcaattagat actttgttag aagaatccct aagaaaaaga aaagggtaga
    18241 caatactttt ttaccaacac ttagccattc tccgtcaaat gcttggatga tcgatagaat
    18301 gatattgcca gtttgagcag aggcaaaagc accatattgg atataagtat aactgtccat
    18361 ggctcctccg ataaatgtca gcaaaagtcc tattgtacgc ccctcgtgaa aggaagtctg
    18421 catttttttc atcacctttt atgaatattt ttgtcttcct tatcttagca taaacgattg
    18481 aaaaaaagga aaaattttca gaaaactaaa atcatatcgc ttcccatttt catttttaga
    18541 aaggtctgtt agtataaaga aaaaatagac aacgaaactt taaataacca acttgaaata
    18601 aacaaattca aagaagaaag atgggaagga aatcaatcag ttgaatgaaa aagaaattct
    18661 ccgattacaa aaagaattga aacgctggga gaacaagtat aaaaagcttc aacaagtctt
    18721 tcggctacaa gaagaaatca ttgctaccta tgaagcggct tatggcgaag cacccggact
    18781 tattgaggaa gaagtacaag taaaagaaag tgaaggaaac agctctttct ttaaaatgat
    18841 ggagaaacgt tcctataatc gcttcaatca agaacaaaaa gcagctatca catatgatat
    18901 gaagaaacat cttcgaatca tagcaggagc tgggagcggc aaaacgcaaa caatctgtgc
    18961 aaaagctgtt tatctgatga cgcaaaaaca agtagacgaa gaacgcatac taatgatcac
    19021 ttttacaagg aacgctgcaa atgaactaaa aaaacgagta gataatttca gccagagaaa
    19081 aaccgctgtc catatcggaa cattccatag tatctttttt aggatataca atgaaatttg
    19141 tcgaaaattt cctgaagtag caatgcaagg aatacaagga gacttttcaa aggatactgc
    19201 acaaaaagtg aatgctgttt tgcaacagct gatacgtaaa tataatcttt atatatttga
    19261 tcaatatgga gaaaaaacga ttgcttctcg gttagactat tggcagaata tgaacctttc
    19321 agtagaagaa atggttcagt tagttaaaga aaaatatgat tccattgata agaatgcacg
    19381 acagccgata tcagaacgac tgtatggatt gctcacggaa ctccaagaac aaaaaagaag
    19441 ccagcaattg ctagagttca atgatgtact ccaaaatctg aaaagcgctt tggagaatga
    19501 ggagatccag cattatatcg gccaaaaata tgattatcta ttcattgatg aatttcaaga
    19561 tactaatcca cttcaatggg aaatcgtgaa acggatgaca aaggaaaaca ggatcaagtt
    19621 gattgttgtt ggagatgatg atcagagtat ctatggattc agaggatcag agccagcata
    19681 catcaagaac tttgaaaaag aatatcctac caaaactctt ttcttattaa ctaattatcg
    19741 ttctcgggca gctattgtcc aaggagcaaa tagattgatc agctataata aaaatgatcg
    19801 gataccaaag gcgatgattc cagcacaaaa agaagcaggt gttatggaag cctatttgtt
    19861 ttctgatact aaagcagaaa gtcagtggct aatcaaacaa attcagacat ttattcaaaa
    19921 aaatgggaaa tataaggaat caatcatatt ataccgatca gcttcacaaa cgcagcagct
    19981 tgtccaatct ctgctgaaaa cgaatattcc ttttgtactg gaagcagata gtccatatga
    20041 aggtatcttt ggtatcaagt ctttccaaca tttctatcaa aaaattgttc attggcaaac
    20101 agcactgaat ctgcaaaaga aaaatcaggc ctaccagcaa ttgctccggc agctgatggc
    20161 agactgctac ctgaaaaaaa gtgaatgcga tcaatacttt tctcctaaat atcaaaatat
    20221 agccgttgca gattatattt tgtcgataag ccccaattta aaatcgaaat ctgcagaaat
    20281 ccgagagttt gaggaagcgt tgcgtaaaac agaccagcat caagaaatct attcgcttgt
    20341 tcaaagctat cttcatctgc cacgtatagc taaggaaatc gaccagagtg atcaagaatg
    20401 gatacgagaa gaagtaaagc agcatgctac tttcagaaga ctccaagcct tatcgcaaga
    20461 aacccaagcg acagctaaag aattaaaaac ccgcttgatt gcttacagac aaggaaaact
    20521 tgatgcacta tgtatccaga gcattcacaa aagtaaaggt cttagttacc gaaatgtctt
    20581 tttgattggt tgtaacgaag gaagtttgcc ttataacgga gcgacagaaa agaaagtaat
    20641 cgactttaat gaaataaaag cagaaccagt aacgacgata gaagaagaac gaagactatt
    20701 ttatgttgcg atgactagag caagaaatcg tctgtatcta tgtgtacctc aaatgaaaaa
    20761 ttcaagaaaa ttgaaagtat cgagatttat caaagaaacc gggagtcgtg tgaaaaaagg
    20821 aaagtgatag aagagagggt gtgacagaag tggtcagttt caagaaagaa gaaggaatgt
    20881 acgaaaattg cttctcaaat ttttgtgaaa tttcagctta ttttcgaaga agctatttct
    20941 ggaacacagt ttattcggaa aagggaggtg tgacaagaat tttgtcacac ctcccttatt
    21001 tattttgaaa atgagaatac tattatcaaa atagagaata tatagaaaaa ttctcattat
    21061 tcaaacgtaa ataatcagtt attttgattt tttttatatt ttattagcta ataattggta
    21121 ttttaaaaga aattactgta gaattggatt ataattaaaa aggagtgaga aaatgataaa
    21181 agtatatact tcgcctagct gtacttcctg tagaaaagca agagcatggt tgcaagcgaa
    21241 tcaacttgaa tttgaagaga agaatatttt tgccgaaccg ttaacagaaa gtgagatcaa
    21301 aaagatcttg gcagcgactg aaggaggagt tcaagatatt atttccactc ggtcgaaaat
    21361 ttacgaaaag ctgggcatag attttaacga attaacttta aagcaaatga tcgtcttatt
    21421 caaagaatat ccttcattat tgagacgacc tattttgatt gatgaaaaaa gaatactaat
    21481 cggatttaat gaagatgaga tccggtcatt tgtccctcgt gaaattcgat gtgttgccag
    21541 aaatctgatc tatttacaaa acaataacat cttaaattca tagggaggga gccttcgtgg
    21601 aagtgaaagt ctatggatca gcgcactgtg taacaagtct gaaggcgaaa caatggttag
    21661 aacaccatca attgccttat caatttgttg acttagaaga acgcgatcta agtacagaag
    21721 aagcagaaga attatgcagt tttgaagaag tgcaagcaga acagctattt gcgacttggt
    21781 ctgaggcttt tcacaacatt gcgattgacc tttcctgtgt aggaaaaaaa caactgattt
    21841 ttttatgcac acaacatacc aatctcctca gacgtccttt gattattatc gatgaagttc
    21901 tatttattgg atacaatgag cagttgatgg aacagcttat cttaaataaa tagactttaa
    21961 ctgttttaat caagtcactg agcatttcat acagttgtaa cagtcgaaca cttagcatca
    22021 agcgtgttat gtgttctttt tttgacttta tttttctact atagagacat gctcaattta
    22081 tgtggtcaaa attcaaatct aggtggggat atagagt
//
LOCUS       DAZKOK010000039        22056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000039 DAZKOK010000000
VERSION     DAZKOK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.039"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..1961
                     /gene="tdc"
                     /locus_tag="ACG90W_001304"
     CDS             126..1961
                     /gene="tdc"
                     /locus_tag="ACG90W_001304"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0477679.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2266..3744
                     /locus_tag="ACG90W_001305"
     CDS             2266..3744
                     /locus_tag="ACG90W_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0477680.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3710..5041
                     /locus_tag="ACG90W_001306"
     CDS             3710..5041
                     /locus_tag="ACG90W_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0477681.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5155..7707
                     /locus_tag="ACG90W_001307"
     CDS             5155..7707
                     /locus_tag="ACG90W_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0477682.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7714..9153)
                     /locus_tag="ACG90W_001308"
     CDS             complement(7714..9153)
                     /locus_tag="ACG90W_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477683.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9128..9310
                     /locus_tag="ACG90W_001309"
     CDS             9128..9310
                     /locus_tag="ACG90W_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477684.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9499..9861
                     /locus_tag="ACG90W_001310"
     CDS             9499..9861
                     /locus_tag="ACG90W_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0477685.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10048..10905)
                     /locus_tag="ACG90W_001311"
     CDS             complement(10048..10905)
                     /locus_tag="ACG90W_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0477686.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11025..11936
                     /locus_tag="ACG90W_001312"
     CDS             11025..11936
                     /locus_tag="ACG90W_001312"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0477687.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11943..13589
                     /gene="mdcA"
                     /locus_tag="ACG90W_001313"
     CDS             11943..13589
                     /gene="mdcA"
                     /locus_tag="ACG90W_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0477688.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13691..13990
                     /locus_tag="ACG90W_001314"
     CDS             13691..13990
                     /locus_tag="ACG90W_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0477689.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            13980..15656
                     /gene="mdcD"
                     /locus_tag="ACG90W_001315"
     CDS             13980..15656
                     /gene="mdcD"
                     /locus_tag="ACG90W_001315"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0477690.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15660..16253
                     /locus_tag="ACG90W_001316"
     CDS             15660..16253
                     /locus_tag="ACG90W_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0477691.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16277..17353
                     /locus_tag="ACG90W_001317"
     CDS             16277..17353
                     /locus_tag="ACG90W_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0477692.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17378..18247
                     /gene="mdcB"
                     /locus_tag="ACG90W_001318"
     CDS             17378..18247
                     /gene="mdcB"
                     /locus_tag="ACG90W_001318"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0477693.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18358..19122
                     /locus_tag="ACG90W_001319"
     CDS             18358..19122
                     /locus_tag="ACG90W_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0477694.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19611..21704
                     /locus_tag="ACG90W_001320"
     CDS             19611..21704
                     /locus_tag="ACG90W_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0477695.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21729..22031
                     /locus_tag="ACG90W_001321"
     CDS             21729..22031
                     /locus_tag="ACG90W_001321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0477696.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7150 a         3776 c         4706 g         6424 t
ORIGIN      
        1 aaaatctacg tggatttgat gttataaaaa aaaaattctc atttttggcg ctatagtgaa
       61 gaggtgttac agataaacac atgtatctgc aagacattga ccaacaaaat atggagggaa
      121 gaaaaatgaa agatatggat atcaaggccg tctttatagg cgacaaagct gaaaatggac
      181 cagtttataa aatgttgttg aacaagatgg tagatgaaca tcttggatgg agagaaaatt
      241 atattccttc agatatgcct gcaattagcg aaggagataa gctcactcca gattatctag
      301 ccacccgcga ccatatgata gaggtcttgg atgaagtcag tcagcgatta cgcgcaggat
      361 cgatcccatg gcattcagca ggccgttact ggggacaaat gaatgctgaa acattgatgc
      421 ctgctttatt agcttacaac tatgcgatgc tgtggaaccc aaataatgtt gcattggaat
      481 cttctatggc aacttcgcaa atggaagcag aagtaggaca ggattttgct tcattattca
      541 atatgacaga cggatgggga cacattgcag cagatggttc tattgctaat cttgaaggat
      601 tatggtacgc ccgttgcatc aaatcgattc ctttagctgt caaggaagtg ctgcctgaaa
      661 aagtgaaaaa aatgtctgag tgggaacttt tgaatttatc tgttgaagaa attttggaaa
      721 tgaccgaaag ctttacggac gaagaaatgg atgaggtcaa agcagcttct tcccgtagcg
      781 gaaaaaatat ccaaagatta gggaaatggc tggtccctca aacaaaacac tattcttgga
      841 tgaaggcgtt agatatttgc ggagtcggct tagatcagat ggttgctatc ccagttcaag
      901 aggattatcg aatggatatt aatgctttgg aaaaaacaat tcgtgaatta gctggacaaa
      961 aaatcccaat tctaggtgtg gttgctgtcg taggaacgac agaagaaggt caagtagaca
     1021 gtgtagataa aatcgtccaa ttaagagaaa ggttaaaaga tgaagggatc tatttctatt
     1081 tacatgttga tgccgcatat ggcggctatg cgcgttcact gtttctaaac gaagcaggag
     1141 aatttgtgcc gtatgcttcc ttagctgaat tctttgaaga acatcatgtt ttccaccact
     1201 gcgtaacgat tgacaaagag gtatatgaag ggttcagagc tatttcagaa gcggattctg
     1261 tcacgataga tcctcataag atgggctatg tcccttatgc tgctggagga atcgtaatca
     1321 aacataaaaa tatgagaaat atcatttctt atttcgcgcc atatgtgttt gaaaaatcag
     1381 taaaggctcc agatatgtta ggagcttata ttttggaagg atcaaaagct ggagcaaccg
     1441 ccgccgcagt ctggacagca catcgtgtat tgccattgaa tgtgaccgga tatggtcaat
     1501 tgattggggc ttcaatcgag gcagctcaaa gattcagaga atttctcgat catctgactt
     1561 ttactgttaa aggaaaaaca atcgaagtct atccattgaa tcatcctgat tttaatatgg
     1621 tcaattgggt attcaaagaa caaggctgta cagatttgaa cgctatcaat gaattaaatg
     1681 aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt ttatggtgaa cggtttatta
     1741 cttctcatac tacatttaca caagaagatt acggtgattc tccgattcgt tttgttgaaa
     1801 gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa aatcactctg ctacgtgcgg
     1861 ctatcatgac tccttatttg aatgatgatc gaattttcaa tttttataca aaaaaaatcg
     1921 ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata gaactgattt ttgtgtgaaa
     1981 gaataacaag agacatatgg agaacagaca attatttgct tcatcaaaac aattgtattt
     2041 ccccctgttt ttcatatatt cctcgccaag atattctttt ttataaaacg ttctataagt
     2101 tggatcgttc gtttcatgta tcaaacccag ttcactaaga tcgttcaatg gatgtttcgg
     2161 tgatactggg ttttgtactg aatcgatgaa accttgtcat ttctgttctt gaacaaaaat
     2221 aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa taaagatgaa agatactggt
     2281 tcatcaaagc aaatcacgac catgaccttt atcggaatga cctgcgccct tgtagcaagt
     2341 gtacgaaata ttccagatgt agcagcaacg agctggacga tgatttttta catgctggtt
     2401 gcagtattat tgtacgcttt tccaatctca ttgatctctg gtgaatttgc tggaatgttt
     2461 ccccaaaaag gtggtccaga actttgggta tcgaatgctc taggaaaaaa atgggggttc
     2521 gttgtctcat ggctgttatg ggtccaaatg tttcctggga tggtgatggt cgcttcagca
     2581 ctcgctccgt tgtttggtaa tattatcgat aatgtgccgc ttggtttgaa cagcaaattt
     2641 acattagttg tcattttagt cgtttattgg atcatcactt ttttgaattt gaaatttgat
     2701 atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat tgtacgtgcc tttgacgatg
     2761 atgctcttat tagggtttgc tgcgtggatc aaagttggga tcgtaccaac cggaacatta
     2821 ggcagttttt catgggacaa attaatacca gatactacga ctgcatcctc atttgtttat
     2881 tttgcgccga tcatgtttat ttttactggt atcgaaatgt cctctgtgta tatcactcga
     2941 ttggaaaatc cagttaaaac gtatattcgc ggagtatttg ccgcgttaat ttttatattt
     3001 ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg taccaaaagg acaaatggaa
     3061 ttgaataata tcgcgcaatc tatttcgatt tattgccaaa ttcttggttt gccacatctt
     3121 attgtgaatc tgtttagttt acttgtcttc attggtgtag cagtccaatt atctgcttgg
     3181 gcctcaggac cagcaaagac agtgacagaa agtgctagaa aaggtgcgta tccaccaaaa
     3241 ttcaattttt ggaagacgaa tcaatttgac gtatccaaat ccgttatttt gactcagtct
     3301 gtcattatct caatctttgc acttttttat ttgctgattc ctggcgtcaa tcaagcattt
     3361 ttgatgctcg taaattcaac tactgttatc tactgtatcg tctatgtcat catggggatc
     3421 gcagttttga gattgcgtta tacacatgca aagttgaatc gaccatttag aattggaaaa
     3481 aaagagaaaa agagcaatct tggagtttgg actgtcgtta ttgttttatt tgcggcaatc
     3541 ggattttctg tcggactgac gatgaaagct ggaacatgga tcaatctaat tgcagtcaca
     3601 gccatcagtg tcattttgtt tgtagtacca ctctgtattg aaaaaataaa gaaaccttct
     3661 tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag gtgaacttga tggaaaaacc
     3721 ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta atcgcattag gtggcgcaat
     3781 tggaacagga ttatttttag gttcagcaaa ggcaattgca tcagcagggc cagctgttat
     3841 tctcgtttat ttgatatgcg gtttggctat ttatggtttg atgcgggcca tgggtgattt
     3901 atttttagca aatagtacat ttaactctat cagtgatttc gttgactatt atttaggcaa
     3961 aaagtgggca ttttttgtca attggactta ttggctttgc tggcttggag ctggacttgc
     4021 ggaattaaca gcaataggat tgtatatcca tttctggttt gtacatgtac cagctttgat
     4081 aagcggaggt agcgcactaa tgatcttaat ggcagtcaac cttgttgcag tcaagtggtt
     4141 tggtgaattg gaatcttttt tctcaatcat caagatcttg gggattttcc tttttatctt
     4201 attgggtata ggattgagtg tctcagattt tcatacccag tctatcggaa tcaaagaaaa
     4261 ttttggacag atcgatgaat tactaccgtt tggctttgct ggaatggtag tctcatttcc
     4321 tatggtttta ttcagttttg ctggaattga aatgatcggc ttaaccgttg gcgagacaag
     4381 tgatccgcaa aagaatttga aaaaagcaat caactgtctg ccttggcgta ttctcttttt
     4441 ctatatagga acgattttgt cgattttatt tgttgttcca tggcattgtt tagatttaaa
     4501 agaaagtcct ttagtgacta tgctgcaagt gatccattgc caaggcggag cagcaattgt
     4561 caatctaatg attttggttg cttctttgtc ctcagcaaat agtgcagtct acagtactag
     4621 caggatttta tatcaaacag caaaagaaaa aagtttgccc cattggtttt gtgcgctttc
     4681 taagaaaggc gtacctttac acggtatttt aattacagga atgttatttt ttgccggtct
     4741 ttgtctacat ttttttgtag ctgatagtcg attgtttttt cagcttttgg caggcatgac
     4801 aacgagttgt tttctatttg tgtggtcttc gatcttgtgt gcacatgttc aatttattaa
     4861 agaaaagaaa cggaacacta cacaaagata taaagatctt gctttgttgg tgttttttac
     4921 ttcaattgct ttcagcttgt tattcgaaag cacgacacgc tttattcctt ttttattggt
     4981 tatctggttt gtattcttaa attttgtata taaaagaata agcaatagaa atatcagtta
     5041 ggcccagata cattttgcaa aacgaattat ttcagacccc cctgaaataa tcgatacgcc
     5101 tgaaaaaact tccctgcgta taataacagt atcaattaaa caggagggga taggatgaac
     5161 tggtacagat tgacggtaga agatgtattg aaaaactatc aaacatcaac agaaggcttg
     5221 tcgcaagaag aacgacagaa acgcctgact gatcaaggat ataatgaaat aaaagcaagg
     5281 cagcagacaa aacgatggaa aaaaatagca aagcatttta ctgatctgct gatgatcgtg
     5341 ttgatcattg cctctttttt gaaatttgct tctagtgaat atattgaagg aagtattatt
     5401 ttattcgtag tgatcgtcaa tggactcgtt agttactggc aagaaagaaa agcggaagaa
     5461 tcattgaatg gtttgaagca gctgatggga caagaagcga tagtactatc aaacgggatg
     5521 caagaaaaaa tccctgcgaa agaactggta aaaggagatg tcattgtctt acgtgccgga
     5581 gatgtcgttc cagcagatat ccgattaatc gaagcacatg atttattagt ggaagaatcg
     5641 attttgacgg gagaatcaga agcatctgaa aaaagtcatg ccgttttgac agaagaagaa
     5701 tcgatcggcg accagaaaaa tatgggtttt tcaggaacgc ttgtacaatc tggttctgca
     5761 gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg gtaaaatcaa tcaagcattg
     5821 caatcggtcg aacaacaaac gacgcctctg attaaaaaaa tcaaccgatt aaacaaacaa
     5881 atttttcaag gcattctttg tttgagccta ttcttagtta tttttacgac atttcgatat
     5941 ggtatggatt ggcatatcct tctttcggct acgattgctt tggtggtgtc aatggtaccc
     6001 gaaggattgc cagctgtttt gaccatgatt ctatccgttg gagtacatga aatggcaaaa
     6061 gaaaaggcga tcatcaaagg acttccttcg gtagaaacat taggttctat gacagtgatc
     6121 tgttcagata agacaggaac attgacaaaa aatgaaatga cagttgtgga tgtagctgca
     6181 aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc aggaattaaa aacaaaagaa
     6241 gaacagaaaa cagaaaattt aagtggcaat ccgacagaag ttgcgctgat tcgacacaca
     6301 gaaacagcca atctaccttt gaaaaaaata gaagcaaaga ttccgttcag ttctgaatac
     6361 aaatatatgg caacgatgca tgcggaggaa aaaggagcgg tgatttacgt caaaggtgca
     6421 ccagaagtat tatttgctaa atcaactctt tcttctgcag aacaagaagc atggagtcag
     6481 acagctgcag aatttgcgag aaaaggtcag cgtgttttag gatttgctta taaaaaagta
     6541 gattcgaaac aggaactcac gcatgagact ttaacccaat taacttttgc agggatcgcc
     6601 ggattgatcg atccgccaaa agaaagtgca gtcaaagctg taaaggaatg ccaacaagca
     6661 gggatctcag tgaaaatgat tacaggggat cacaaagaca cagcaaaagc gattgcggaa
     6721 caaattggct taaaacatac tgccaaagtt ttagaaggga tcgatttgga tcttatgtct
     6781 gatgaagagt tgattcagca agtgccaata gtcgatgtct ttgctcgaac gacaccagaa
     6841 cataaactgc gaattgttaa agctttgcag aaaaatggag aaatcgtcgg aatgaccgga
     6901 gacggcgtaa atgatgcacc tgctttgaaa agatccgatg taggaattgc tatgggaatc
     6961 aaaggtagtg aagtaagcaa acaggcagct gatatggttt taggagatga taatttccat
     7021 acgattgcaa aagccgtaaa agagggccgg cggattttgg ataatttgca aaaaaccatc
     7081 aatttcttct tgccaactgc attagctcaa ggattgatct tgatttgggc acttatgctg
     7141 aatcgcccgc tacctttgtc cccagtacag attttatggg tcaatatggt aacgacaatc
     7201 actttatctt atgcactagg gttcgagtcg ccagatccag aaatcatgaa gcgcccacca
     7261 agagatccaa agcaaggaat cctatctggt tatcatttgt ttcggatcat ctatgtctcc
     7321 ctactgatca tggttcctgc ctatttattg gctatgcagt ttgaagggca aagcttacag
     7381 caaaccattc ttctgcaaaa tatcgtctta gctcaagctg tttacatgat caattgcaga
     7441 gagttgtcca agccgtcatt gaaccaaggt cttttgcaaa acaaagcgct ttttctttca
     7501 ttaggtatcc ttgctttatt gcaagggatg gttatttttc taccagtcgg ccagcagctg
     7561 atcggtacag taagtttaac gatgacacaa cagttattga ttggagcaaa tgtgattctt
     7621 ctattcttag ttgtagaaat agaaaaaggg atcatgaaca aactgtttcg caaaaaagaa
     7681 aaaacagcta gagaatacag tcgctagccg ttactataaa aaagttttga aagtgagttc
     7741 agtcaaacga ttgagctcat ttttcgttgg aatagaagaa atgtatacaa tagggatatc
     7801 tagagaatct agagggaaat tggaaatcaa gatatctgtt ttttcaaaag aaatagtgga
     7861 aagatcggtt agttcgatag gtaagaagca gacacgtttt cctaagtaat cttttaattc
     7921 ctgctgcaag aacgccttcc atgaaggttc tccttggaaa ataaaataga cattgaacaa
     7981 attcggtttg atagaaaaaa tcgtttgttg gatcagtaga gacaagagtt ggagaaagta
     8041 atttggttca ttgatttcta tttctttttt tagatcgttc agtaaattct ctttctgttg
     8101 atacaagtgt gcatattgct gatcatatgt ttctaaaaaa cgatggtatt gacggtccaa
     8161 tgcttcaatc aatatcggcg attcatgata ttttaatatg aggagagcca agttatcagt
     8221 cagagatgtt ttttttagtt gatcaagaga cagctctgaa gctagttcct ctacaaacgt
     8281 ttcgaggacc tctaaaaatg gagaatgatg caggcagcga gcggtttctt gtccatagtt
     8341 attgttgtaa ttccaacttt ccagaaagca gaaagaagca aaagaaagct cagtgtcttt
     8401 aagataaata ccttccgtta aatacaattt tttcaatgca gagtcataca gggaggagag
     8461 tgtgctgttg gtatggatag aagaatctaa tcggcaattc gcttttatac ggatcgtatt
     8521 gataaagaac caacatgttc cgaaaatctc ttctgtttca accgctagtt cagtttgact
     8581 gaggtttctt aaaaattgaa aataatggga ttcatggatc gaataatcct cgaaaaaata
     8641 gttgttatga gtgaaaggaa tcagcaatcg atgataaaaa gtacgaatcg cacgctcttt
     8701 tccaagaata caagctgtat atttcgtcaa atcgatctgc aagtcgtaac ttgctaaacg
     8761 ctgattgatt ttagtcacat gtcttttcaa tgtcccataa gaaagcccat ggtcggctag
     8821 aaaatcaggg acagcaactt tttttgtaaa aagcagattt ttcagcagtt cgacactgac
     8881 tgattgtttg aaaaaaatct cccaaaggtc atttgttaat aaattttgct ggttttgcaa
     8941 actgattcct gcttgatcgc tgatgatgtc ccagccttcg ggcagctgag agcgaataga
     9001 ttgaaggtca gaaaaaatcg ttcgttcggt tgtccctatg atttcagcta attcatttac
     9061 ggttatcttt gtatgattca gcaattgttc tactaaagaa acatgacgtc gaatatcttt
     9121 ttcggtaatg atttttttca ttagtgaata catcaatcag actcccttct attctaagaa
     9181 tacggagaaa ataaacggtg gtcaagtttc aactgatttt tcaatcattg tttatttttc
     9241 tggagcattt actgatgata agaatttcat catgaacttg aaaaatgata ccactaaaat
     9301 caaaaaatag gtgggcaaac aataataaac attttaatag tagatggtag caagaccaac
     9361 acaggcggat tgaacattta agagttgata aaaaagaaaa atatcgaagt atttgattag
     9421 caactacttg tattcatttt tctattctta cgtggtatct ttgaataaag cgaatcaata
     9481 agaaagagat ggaagcagat gctaacgaaa aaagaactaa tcgatcacat caatgaaaca
     9541 tacaatttgt ctccagaata tatttttaaa aaatttcctg attattgcgt attcagacat
     9601 caaaaaagca aaaaatggtt tggggtcctg atgtctgttc cagaagaaaa aatctatgat
     9661 gatgcaagtg accataggat catcaatatt cttgatttga aggtcgatcc tgaacttggc
     9721 ggaattttgc gaggaaaaga ggggtatttt ccagcctatc acatgaataa agaacattgg
     9781 atctctattg atctacgtaa aatagaaaag ctagatgaac tagcagattt gattgatgga
     9841 agttttaaat caacgcaatg aatgtctagg acaatagaga agaggttgtg acagaaatgt
     9901 ttagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgggaaattt
     9961 cggcttattt tctaagaaat tacttctgga acaccgtttg ttcgaaaaaa gagctgcgac
    10021 aagaattttg tcccagctct ttttttttta ttttcctaaa taaaattccc gcatcgtttt
    10081 tgcaatcatt ttgatcaaag gatgatcttc tgtgaagcta gaagataaat aaattgttcg
    10141 tgtcaagggc aaatctttga tttcccgcaa gacgaaagga tcatttttat cgtaagacca
    10201 agaaactttt gggaaaaagc taacagctaa tccttgcttg acaatagaac gtaatgtcgc
    10261 aggatcatct gtcgtcgaac tgatcgtcaa ttcgatactt ttcgcttcaa gtgctgcatc
    10321 cagtgtatga cggaaggcgt tgctctttga cagcatcaag atgggggtgc gttggatatc
    10381 ttctaattgc agatgttctt ttttagcaag aggatgtttc ttaggaatgg cgacataaac
    10441 aggttctttc atgataggaa tattggtcat gccaggcttc atctccgaag tgatgtacag
    10501 tacaggatcg gtaatatcat ctggtaaatg atgttgcatg atcgttaatt ggatatcagg
    10561 atgcttttcc ataaaaattc ggaccaagtt cggaatagaa acagaggcta cctctaaata
    10621 caatatgacg gaagtaacga tcttctcatt gaccgaagac aataatttga ttccttcttc
    10681 taatgtgtct aatgtcttga ctgcatatgg ataaaattct tctgcataaa cggtcgtctc
    10741 aatgtttctg ccgctatggt aaaacaaagg gacacctaat tcgttttcca aatgtttgat
    10801 cgcacggctc aaggcaggtt gagaaataaa taattctttg gctgcttggg taacattttt
    10861 tgtttcgaat acttttttaa aatactgcag atgaagcaat ttcataatta tccatcccat
    10921 ccataccttt ttgttatggt tttttctttt tttggcattt tttattatag cagaaattga
    10981 ctaagatagc agtggaaaaa gtaagtgctg aggaggcgag gctcatgagt acaggtttct
    11041 tatttcctgg acaaggcagt cagaagttag ggatgttagc ggatatccct gaaatgtaca
    11101 tggaacgttt agaaaatgtg acggggtatc ggttagttga taaagaagaa aactatcaag
    11161 atacggtttt tatccagcta gcattactga caaaagcagt attttattta gatgaaatgg
    11221 aaaaaaacgg tattcagcct gatattgcag caggacattc gattggtgct ttttgtgcag
    11281 ctgttgcttg tcgatctttg agatttgaga atgcagcgga actagtctat caccgcgcag
    11341 tcttaatgaa agaagcttat ccaagcggtt atgcaatggg cgtagtagta ggtgtgacga
    11401 gaagcgaagc tgaggaaatt gtagcaaaaa catttgatcc tgaccatcca gtttatctgt
    11461 caaatgaaaa ttgtccgatg caacatacgt tatctgggag tatcgaaggt ttatccgtta
    11521 cgttggcaga agccaaacgg caccaagcgc aaacagcgaa gctgctgaaa gtccctgtgc
    11581 catctcactg tctcttgatg aatgatacgg tcaagaaatt caagccgtat atgcagtctg
    11641 tccatctatc tgatgctaga tgtttgtata taaaaaatac ggatggaaga gccactcagg
    11701 acgcagaaga gattcgtaaa gatttgttgg aaaacctagc tttccctgtg cagtggaacc
    11761 aaatgatgga cttggcaaaa gaattaggaa tggatctgac gattgaattt ccccccggca
    11821 atactttgac aaagctgatt catacaaaat ttggtgtaga taaagggatc cgtacgatca
    11881 atctagacca acatggtatt gatgacgcca cgtttctata tcaaaaatgg agatgactaa
    11941 ttatggaaaa accaaaccgc aactgggctt ctaaacgcga aaaaaaagcg cagcgattgg
    12001 aaaaaatcag caacttgctc aatggcaagt atgtagaaac tgaaaaaatc gtagaagtaa
    12061 tggaaaaact gattgctccc ggcgacaaag ttgtattaga aggcgacaat caaaaacaag
    12121 caagttttct ttcagaaagt cttgaaaaag tagatgcaga aaaagtgaat gatctgcatt
    12181 tgatcatgtc tagtatctca cgaccagaac atttgaatat ttttgaaaaa ggaatcgcaa
    12241 gcaagatcga tttctcttat gctggtgcac aaagcttgcg tgtcgcccaa atgattgaag
    12301 acggcacaat gaaattaggt gatatccata cttatttgga actttatgga cgcttattca
    12361 tcgatttgat tccaaatgtt gttttagtag ctgcagataa agcagataaa gacggcaact
    12421 tgtacacagg tttcaatacg gaagaaacac caacaatcat cgaagctgca gctttcaaag
    12481 atggtatcgt gatcgttcaa gttaacgaag ttgtcgattc tttaccacgt gtagatatcc
    12541 ctggtgactg ggtagacgtt gtcgtgaaag cagataaacc ttatcaatta gaagctttat
    12601 tcacacgaga tcctcaaaac atcacagaat tgcaaattct aatggcaatg atggcaatca
    12661 aaggaatcta cgcagaacac ggcgtacaat ctttgaatca cggtattgga ttcaatacag
    12721 cagctatcga attgttactg ccaacttatg gtgaatcact agggttaaaa gggaaaatcg
    12781 cgaagaactg ggtattgaac ccacatccaa cgatgattcc agccatcgaa tcaggttggg
    12841 ttgaaagcat ccatagtttt ggtggcgaag ttggtatgga aaaatatatt gctgctcgcc
    12901 cagatgtctt ttttgtcggt aaagacggca cgatgcgcag taaccgaaca ttatcacaag
    12961 ctgcaggaca atatgccatc gatatgttta tcggttcaac cttgcaatta gattacgaag
    13021 gaaactcttc taccgtaaca aaaggacgtt tatctggttt tggcggtgcg cctaacatgg
    13081 gacataatcc tggcggtcgt cgtcattcta caccagcatg gcaatcactt cgcgacaatg
    13141 atgatccatt agggaaaggc caaaaattag ttgttcaaat ggttgaaaca tatggttcaa
    13201 ataaaaaacc agtctttgtc gaagaattag atgcactagc tgttcaaaaa caagcaggct
    13261 tagccaatgc accagtgatg atttatagcg aagatactac acatatcgtg acagaagaag
    13321 gaattgctta tttgtacaaa gcaaaaacaa aagaagaacg acaagcagca attgcagcaa
    13381 ttggcggtgt tacacctctt ggaatgacta gcacgaaaga atcactagat agcttgcgtg
    13441 cagccggcat cgtgaaatta ccagaagatc taggcatcaa acgtagtgaa gcgaaacgtt
    13501 cattattagc tgcacaaacc atcgatgatt tgatcgaatg gtcagatggc ctgtatgaac
    13561 caccaatgaa attcagaact tggtcataaa attggcgaga aaaacctttt gtcgccattt
    13621 acccagatgg aagaagaaag acgaataagc agaacgtgta tgctctgttt tacaaaaagg
    13681 ggagagatcc atggaacaat tagcttataa atatccaacg aaacagccga tcaataagaa
    13741 aatccatgta ggagtggtcg gttcaggaga tttggaaatc ttgatgtttc caacagaaaa
    13801 aacagaatct actgtaaaaa tttgtacagg aagcgatggc ttcggcgaag tatggaacaa
    13861 tgtattaacg agattttttg atcgttatcc aatttcagca gatatcgtga tcaatgactt
    13921 cggtgctaca ccaggagttg tgtacctacg attgacgcaa gcaatggagg aactttcaga
    13981 tgaaaaatag ttttgtagaa ttaaacgcca gagaacgagt gaacgctcta cttgacaatg
    14041 gacgtgaatt gattgatcca ttcgatcaaa tcatcgcgcc aagtttacca gcacaaggaa
    14101 tcgtccctga aagtgatgac gggatcgttg tcagccgtgg cacgatcaat ggcaaacaag
    14161 cagtagtaat cgctatggaa ggaaaattcc aaggtggcgg tatcggtgaa gtttccggtg
    14221 caaaaatcgt tgctgcactt gaacatgtgt tagaagaaaa taaaaaagga aatgagatct
    14281 ttccagtcat tgtactagat acaggtggtg ttcgtttgca agaagcaaac tacggactgt
    14341 tatctatttc agaaatcgga aatgtaatca ttgctctaaa acaatatgta ccagtcatcg
    14401 gtttgattcc aggacgtgta ggatcatttg gcggtatgtc gattacttca gcattaatga
    14461 gttatttaat cgcaacaaaa aaagcgagag tcggtttgaa tggaccagaa gttattgaac
    14521 aagaagcagg cgttcgagaa tttgattcta gtgacaaaga cttgatttgg aatacattag
    14581 gatctcgtca acgggtagaa tcaaaaatca ttgatgaatt agtgactgat agtgtagaag
    14641 caatcaaagc tgctgtcatc gcagcaatgg acgaacgaaa agacacgcat cgcagtgaaa
    14701 gctcagcatt ttatttatca ttacttgatt ctttagatct ttcaaaacca atggccattg
    14761 acgattacaa tgaagcaatt gctaaccatc aagcaaaatc aattgactta ccagaagtag
    14821 cagaaggaaa tgaaccagct gcagcaagtg ttggttacga ttggtttgcg gcactaactg
    14881 gcatgaaaaa tccaacttct tctattagta ctgtgtatta tggtaaatca agtaaattca
    14941 tagaagaagc catcgtgaca tcaatcgtac ctgatcctaa aaacccgatg taccgtgtac
    15001 gaaacggtga agttggttta gttgaaggtt tccgcatggg acaaattttg gatcacgttt
    15061 acgaagaaga taaagacaaa aaagtgaaac ggccaatcat cgttgtgatc gatgtaccaa
    15121 gccaagcata cggctataac gaagaattga tcggtatcca tgtggctttg gctaatagtg
    15181 ctgctgctta tgcaaaactc cgtcaagctg gacatccagt tattggattg attgcaggaa
    15241 atgctatttc aggtgcattt ttagcacatg gattacaatc aagccgcttg attgctttaa
    15301 acaataaaga aattaccgtc caagcgatgt ctaaagaaag tgctgcacgt gtgacaaaac
    15361 gttcggttgc tgaaatcgaa gcagctgctg aaaaagttcc agctatcgct tacgatatca
    15421 ataattatac aaaactagga gcgatctatc atttcctaga agatatcacc gaccaagctg
    15481 cttcagaaca aaatgtcagc aaagtcatcg aagcaatcaa tgaagccatt caagatgtcc
    15541 gcaatacaaa tgacacgatg ctgacaaatc gatacacaaa cgaaacagcc aaaacattcg
    15601 gacgtgtcga aacaaataaa gtccgtgcca aaatggacga agaatgggat gcataaaaaa
    15661 tgattcaagc acatgatatt gtcctttttg ataaacatga aatagaaggg ttcaccttgc
    15721 ctgaatgggt cgttctaact gaaaagatgt tcggaacagt tcgtcgtgcg tcgacggatc
    15781 acgcagaaat actaccgatt ggtttacgtg gacctgcccg caatcagaga tggtcaacag
    15841 aaatctcaaa acatgccgtc aaacaagtca ttcatccgtg ggacatcatc gaacaagaat
    15901 cgtatagaca agagaaaatc atggattatc ctgtttatca gcaatttcat gctgctagaa
    15961 acatattatc aggatgtaaa tggggaatcg gcggcagctt aggatttgaa ttatcgactg
    16021 ggattccggc agtaaaagaa accagtgatt ttgatttgct gctgtacgca aattcaccga
    16081 ttgaattgcc aattcaggta attcaatcgc atcctgcttt ttttgagcaa ttcgataccc
    16141 aagtagtgac tcggcaaggc ggtttttcac taaaagagta tttgagagag ccagagaaaa
    16201 aaattctatt gaaaacagtc gaaggaccta aattaaccaa agaactttgg taataaagaa
    16261 aaagagagag aaagagatgt ttgaaaacaa aaaaatcaaa atcaacgtaa gtgacttcca
    16321 gaaagtatca gctgtaacgg ctgttatgat gcaaacgata ttgagctttg ctttggcaaa
    16381 cagccacagc gcaacaaata cagcttttct tggtaccttt tacgtcatcg ttaaatacac
    16441 agcaccaatg tttatttttg caattgttta taatatggta aaaacaagtc agcatttgtc
    16501 ttattgggaa tttttgaaag aaaagttttt tgaactagtc gttccgtatc ttctatggac
    16561 gacggcttat ctttggctat ttccagcagt ccaacaacaa actccgtata cggatactgc
    16621 aagcttttta ctgaaatatg tcacaggtga tggagcgcct catttatggt ataccgttat
    16681 gatgctgcag atccaattgt tcatgccgtt ttttgtttgg ctcggttaca aagtatttgc
    16741 taataaaaaa tatgtatggc cagtattgat tggttcaacg attttatatg tcgcatggta
    16801 tctgttctat caaacacaag tattgactgg gccttatcat gacagctggt acttattaga
    16861 tcggtttgtt gctagtttca tgatttatgg ggtagccgca tttgtttatc acgaaaaagt
    16921 ctatcaatat ttggacagag tacgttatct tttcttacct gttgccttag ttatcgcgtt
    16981 cttttctgtc cgttcattgc tcgcacatcc aggagacttg agctttgcga atgcacctta
    17041 cttgaatacg atccaaagtt tatacagttt agtcatcatc tttgctgtct tcataggcgc
    17101 ttccaaaatg attgtaaacg attcgccaaa attgccattg ttcaaatggt tgtctgttta
    17161 tgcttatcgt acgtatttag ccaatgtatt tgttttccaa gtactgttat tattgtttaa
    17221 agatagttgg ctgcagttac cttcaggagt catgattctt gtagcttatc taatgacggc
    17281 aagttgtgca tttgctttaa gctggttact tcatatcatt tgggtagcta tcaaaaaagg
    17341 tttcagcaag taatagtagg aagaaggagt gagtgtcatg ataaatgacg aacgaaaaaa
    17401 agcagtgact cagcaactcg ctgcgttggt agaggaagca ttgatcgcag aagtaacatt
    17461 aagtccaaaa cctggattag tggatgcact ggatgcgggt gcacattccg atatggacta
    17521 tgctttattt cttaagtctg ctaagacgct aactcctttt tttgaagaaa tggctcaaat
    17581 agcatggcat catgctattg atcaggagtt aagagaaaga attgctgaga ttgggcgaaa
    17641 agcggaaaag gcaatgctga ttgctacaaa tggcatcaat acccataaag gagcgatttg
    17701 ggcaatgggg ctattgatca gtgtcattgc acaaaaaatc agtatggaac agcctttttc
    17761 tttatcgaaa atacttgatg atgtcagcaa gcttgctgct tttcctgata caaaatatcg
    17821 tcataaaaaa accactcatg gtcatagggt gaagcaaaaa tatggcgtaa atggtgcata
    17881 tgaagaagcg atattagggt atccgcatat caggcttgca attcaagcag ctaaggagct
    17941 agataatgat tcatatgtac agaaacaaat gcatatgttg ttagttttga tgagttcgct
    18001 agatgacaca tgcgtattgc atcgcagtga tcagaaaacg ttagtggaaa tgcaaaaact
    18061 tgctcatgct gctagtcaaa gtgctttacc aaatcgtcca tttacagcac tgttgcactt
    18121 ttgtcaggaa aaccaaatct ctcctggagg aagcgcagat ctcttgtctg caagcttgtt
    18181 catgctgaag atagaggaat tatggactgc cgacacatac atttgtccga tgttactcaa
    18241 tgactaaata agacaaaatt gctaaaaaaa cagtttatgc atctggttat tgactcttcc
    18301 ttttagcaag gctatcataa aaatagtttt ttgtaataaa agataacgag gaagacaatg
    18361 aaaaaaatca attatttagc aatcattttg ttagtgggcc tatgtttgtt tgctctgccg
    18421 acccaaggat tcgctgtcgc gggtggtcat ggtggaggta catctagcgg tcgaagttcg
    18481 ggcagaggtt catttggtgg tcgttcatat gggggcagtt attttggcag tcgttcttac
    18541 ggcagagatt cttatgggta ttatggtcca ggtacaggtc cttctagtta tgagcaaacg
    18601 ggatttcttg cattagctgt tttgtcagta ggaggggttt ctgctttttc tatcgtcaag
    18661 aataaaagac aaaagaaaag gctgaaagcg gagatattgt cccgcatgtc aggaacggct
    18721 aaagaaaaga aagcacagtt tctggaaatc caaaagatat tcatgaagat ccaatccgct
    18781 tgggaagaag aaactcctga aaaagccaga agctgttata cagacaagct gtttgccgaa
    18841 catcagaaag ttctgcaaaa aaacaaagca gacggtgtaa gaaatcatac taaaaaaata
    18901 actattcagg aagtaggaaa ttatcgacag atccacgaaa atagttttgc catccgaatt
    18961 gatttcagat gctgtgatta ttcagtcgat cgaaaagatg ggcgattgat ctcaggaagt
    19021 aagacacaac gacagtattt ttctcagata tggtattttg atttttctga gaaagaaaaa
    19081 agatggcaag ccgattttat tcagccgatt atgctggatt gacaggaaat atttatgaac
    19141 ggagttgtga caaaagcctt gtcgcagctc ctttttctga ataaacggtg tgccagagat
    19201 agcctatcgg caataaggca cgattttgta aaatatggga agcgattttt caaaatcgct
    19261 tcttatcgct caaggctgaa catctctgtc acaacctctc cactaaatgg tgtttaaaag
    19321 cttgcatctg cgataataag aacaaccaaa cgaaaaatag gaagcactat tttagtttag
    19381 ttgttcttat tacttgatgc aggacagctt tttcacaacc tcttttttgt atagatgggc
    19441 aaaatttttg taataatagg cgtatgtctt tttcgttgag gagatgaaaa agcatatggc
    19501 ttattttttg tttgctttgc ttactttctt tccacttcaa gtggcagaaa tatctacttt
    19561 ttattatgta agcggaacca tatacttgaa atcagaagga gatcatttct atgaaaacaa
    19621 gaacaattga aatacttcac agaatcatga cagcccaata tccagtaaaa gcaaaacatc
    19681 tagcaagtga gttcaatgtc agtcagcgga cgatccgtca ggaagttctt gaagcgaata
    19741 tctggttgag cagtaaaaag cttccggaga tccgtacaat tcgcaacaaa ggttttttgt
    19801 tgaagctgga aaaggaaaag caagctcgat tagaaactga aataaaagat gtgaaagacg
    19861 gctttttgaa tcggcgcgaa cgtatttttg atctagtttt agccgttgct tacagccgaa
    19921 tcccgatcca tctaaacaaa aaagaagaag aatttcaaat ctctaaaagt acattagatg
    19981 aagatatgag acggttacga gcaacactgg tcagttatgg gatagagatc gtcagttatg
    20041 gaaagcaagg actggtttat aaaggttcag aacgatctat ccgtacgatg atctatgatt
    20101 ttatcaatca gaatctggga atgaccgact tctttatgaa tcaagatgca caattaacac
    20161 cggctcaaaa aatctttctc cattattttc ctttggtgga aatcaaaaaa atacaggaga
    20221 tctattccaa taagttgttg aaacaagaag atgatattta taaaaaacaa atcctgcttt
    20281 ttacattgat ctggatccag cgaatcagga aacatgaatt tatctctgcc attaattgga
    20341 aaaatacaga aataaaagac agtagctttt ctgcatttat tgaccacatg atcgaggaat
    20401 ttcaattgga caatgtcccg caagtagaaa gaaattatct tcgtttcact attgagacgt
    20461 tcaatgctag agatatctcc aattcaatcg agtgggtaca agctcaatta ctaacgatcc
    20521 aattgatcca atttgtagag aacgagacac acgtaccttt ccatctaaag gaagaagctt
    20581 tattcgaaaa tctctataag cacatggctg cactgattgt aagaatcaag aatcatatcc
    20641 aagtcatgaa tccattgaaa gaaaacatcc gaacaaatta tttcccaatc taccgcgcag
    20701 tcactcattt tgttcctacg atcgaagaag taattggtgg caagatactg gaggacgaga
    20761 tagcttttct tgtcatccac ttttcgacga tcgccagtgt aatcaagcga gacttgaatt
    20821 atgtttataa atcagtcgtt gtctgtaacc atgggatggc tactggaaat ctgttagcag
    20881 agaatctgaa agaaaagttt cctcagatcg agattactgc agttttaagt tcaaaagaaa
    20941 tcgaattagt agataaattg gatgtcaatc tggttttcag cacctttcaa ttgaattatc
    21001 gaaataagcc gcttttagtt gtcgatccga ttttaactga gacgaatatc ttggttatag
    21061 aagacttttt gaaaagaaat gaagaactac agcggttaga gcctaacagt catgaatcga
    21121 cggagttatt cactcaaata atagatgtca tgaaagaaag tggtggccga gtagatcgaa
    21181 tcatctatgg caagttagaa aaattattcg aaacaaacaa tttagaaatc aacaaaaggg
    21241 agatccagcc tatgttaaaa gatattttga cggacagcaa tatcatgatc aaagaaaaag
    21301 ccactacttg ggaggaatcg attgaactag ctgctagacc attggttaaa gaaaacattg
    21361 tcgaagagcg ctatgtcgaa gcgatgattc gtgcagtgaa tgattacgga ccgtatatcg
    21421 tgattggcaa acacatggca ctagcccatg cccgaccaga agatggggtc aataaattag
    21481 gagtcagcgt agctacgatt gagcaaccaa tcgatttcgg taatcctgaa atggatccag
    21541 taaagattat tttttgtttg gcagcagtag actcctactc gcatctgaac atcatgaaag
    21601 aactaattga attgatcaac aacgaagaga agttggatcg attgattgaa tgcacaaata
    21661 tcgaagattt caaacaactg cttttttcag tagttgagaa ataataaatg atacggggga
    21721 gaacaaagat gaacaaattg aacattttat tcgtatgcgg tgcaggttta ggaagtagtt
    21781 ttgcagcaca gatgtctgct gaggatgttt tgaacaaatt aggagtagaa gccaaactgg
    21841 accattgtga tatttcttcc gcagtttcca tgaaaccaga tgtgatcatc acagcagaaa
    21901 attttcgttc gcagttcgaa aagttcgcag ttgatcctaa aacggcgatg atctatttga
    21961 aaaatattgt ttcaaaaaat gaaattgaag aaaaaatcac cccagttctg caagataagg
    22021 gagttatcta aatacaaaaa gaaaagaggg taaaaa
//
LOCUS       DAZKOK010000040        21880 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000040 DAZKOK010000000
VERSION     DAZKOK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.040"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..1114
                     /locus_tag="ACG90W_001322"
     CDS             110..1114
                     /locus_tag="ACG90W_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477697.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1115..2059
                     /locus_tag="ACG90W_001323"
     CDS             1115..2059
                     /locus_tag="ACG90W_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477698.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2059..3021
                     /gene="opp4B"
                     /locus_tag="ACG90W_001324"
     CDS             2059..3021
                     /gene="opp4B"
                     /locus_tag="ACG90W_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0477699.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3048..3962
                     /locus_tag="ACG90W_001325"
     CDS             3048..3962
                     /locus_tag="ACG90W_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477700.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3988..5769
                     /locus_tag="ACG90W_001326"
     CDS             3988..5769
                     /locus_tag="ACG90W_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0477701.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6117..6803
                     /gene="rnc"
                     /locus_tag="ACG90W_001327"
     CDS             6117..6803
                     /gene="rnc"
                     /locus_tag="ACG90W_001327"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0477702.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6823..10404
                     /gene="smc"
                     /locus_tag="ACG90W_001328"
     CDS             6823..10404
                     /gene="smc"
                     /locus_tag="ACG90W_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0477703.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10413..11222
                     /locus_tag="ACG90W_001329"
     CDS             10413..11222
                     /locus_tag="ACG90W_001329"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0477704.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11233..12231
                     /gene="ftsY"
                     /locus_tag="ACG90W_001330"
     CDS             11233..12231
                     /gene="ftsY"
                     /locus_tag="ACG90W_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0477705.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12418..14685
                     /gene="gshAB"
                     /locus_tag="ACG90W_001331"
     CDS             12418..14685
                     /gene="gshAB"
                     /locus_tag="ACG90W_001331"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0477706.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14774..15226)
                     /locus_tag="ACG90W_001332"
     CDS             complement(14774..15226)
                     /locus_tag="ACG90W_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0477707.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15376..16326
                     /locus_tag="ACG90W_001333"
     CDS             15376..16326
                     /locus_tag="ACG90W_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0477708.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16319..17278
                     /locus_tag="ACG90W_001334"
     CDS             16319..17278
                     /locus_tag="ACG90W_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0477709.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17275..18030
                     /locus_tag="ACG90W_001335"
     CDS             17275..18030
                     /locus_tag="ACG90W_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477710.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18053..19006
                     /locus_tag="ACG90W_001336"
     CDS             18053..19006
                     /locus_tag="ACG90W_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0477711.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19650..19946)
                     /locus_tag="ACG90W_001337"
     CDS             complement(19650..19946)
                     /locus_tag="ACG90W_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0477712.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20301..20657
                     /locus_tag="ACG90W_001338"
     CDS             20301..20657
                     /locus_tag="ACG90W_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0477713.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20667..21566
                     /locus_tag="ACG90W_001339"
     CDS             20667..21566
                     /locus_tag="ACG90W_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0477714.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         7237 a         3861 c         4661 g         6121 t
ORIGIN      
        1 ttcattacac aaagataaga ataacctttg ttataaaacg atttattctg tgtaatgtcc
       61 ttttttatta agaaaaactt ccatttagga agcagagagg ggtattcgat tgtccgataa
      121 ccaattatta gaagttcaga acctacatac aggattccga ctcaaagatg actattacga
      181 tgcagtagat gacgtttcat ttactttaga caaaaacgag attttagcaa tcgttgggga
      241 atctgggtgc gggaaaagta ccttggctac tacgattatg ggattacttg atcctaataa
      301 cacaaaaatt acaggtgaga ttttatataa cgatctgaat ttaatcgacc tgaacgaaac
      361 gctctataat aaaatccgcg gaaatgatat cggaatgatt ttccaagatc cgttgtctgc
      421 attaaatcct ctcatgagaa tcgaagacca gatcaaagaa ggactttcct atcacacaaa
      481 gatgaatgca gaacagcgtc aagctcgtgc actagaattg ctagaacaag tagggattcc
      541 taatcctgcg agagttggac gccaatatcc ccatgaacta tcaggcggta tgagacaacg
      601 tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc gtcattgcag atgaaccgac
      661 gacagcccta gatgtaacca tccaagcaca gattcttgat ttactgaaag accttcagga
      721 agaaacaaaa acagggatca tcttgatcac tcatgactta ggagtcgtag cagaaatggc
      781 tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa gtagcaggtg taaaagaact
      841 gtttgatcat cctaagcatc cttatacacg ctctttactg aactctatac cacaagaagg
      901 aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt ccttctctga aaaacatgcc
      961 tcgtaaaggt tgtcggtttg caccacgtat tccatggata ccagcaagcg atcatgaaga
     1021 agagcctatc cttcatgaag tcgcaccgaa ccacttggta cgttgctcct gctataagca
     1081 tttccacttt agagacgaaa aaggagaggt gtagatgaca gaattaatca caatcaaaga
     1141 cctgaaagtt cattacccta ttcgcagtgg attcttcaat cgagtaacgg atcatgtata
     1201 tgctgtagat ggtgtggatt ttattatcga aaaaggaaag acatatggtt tagtgggaga
     1261 gtctggctct ggaaaatcta ctacgggaaa agccgttgtg ggattagaaa aggtaacagc
     1321 cggacaaatc atgtatgaag gaaaagatgt aacgaagcga agcaaccgca aaaaaatggg
     1381 ttataacaag gatgtgcaga tgatcttcca agattcaatg tccagtttga atcctaaaaa
     1441 gcgagtgttg gatatcattg ctgaacccat ccgaaatttt gaacggttaa gcgatcaaga
     1501 agaaaagaaa aaggtaaaag gactgctaga tatcgttggg atgccggaag atgcgctata
     1561 taaatatcct catgaatttt caggcggaca acgtcaacgt ctaggagtag cccgtgctgt
     1621 tgctaccaat ccaaaactga tcgtagccga tgaacctgta tctgctctgg atctatctgt
     1681 tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa gaatacggcc tcagctattt
     1741 attcatttca catgatttag gtgtagtaaa acacatgtgc gacaatatcg caattatgta
     1801 caaaggtcga tttgtagaaa tcggtacaag agaagacatt tataatgacc cgcgacatat
     1861 ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat gtagaaaata gggaaagcca
     1921 caaagaaaat cgacgaagag tagaacaaga gtatatccaa aaccaaaaag aatactatga
     1981 tgcgactggt cgagtctacg atttgcgtac gatcacaact acacacaaag tggcgttgaa
     2041 agatggaggt gcaagctaat gtggaaaacg attctgcgac gtgttttatt gatgatcccg
     2101 caagttatta ttttaagtgt tctgatcttc ttattagccc aagcgatgcc gggggatcca
     2161 tttaccggtt tgatcaatcc aaatcaggat ccggcagtga tcgaacagat gcgacaagca
     2221 gctggcttga atgacccatg gtatgtccag tacttccgtt ggatcggaaa tgctttgcaa
     2281 ggcgattttg gtcaaagttt cttatacaaa cgctctgttg ctgacttgat cggcgaacgg
     2341 atcatcaaca ctttatatct atcgatcttg acagttgtcc ttacttattt gattgctgtg
     2401 cctctgggga tgcttgctgg ccggtatcaa aattcaatct tggataagat tgtcgtaatc
     2461 tacaacttct ttagttttgc tgttccttta tttatcttcg gcttgatcat gctatttgtt
     2521 tttggttatc gtttaggctg gttcccaaca agtggatcac aaacactagg tttgcaaggt
     2581 ggatttatca ctcaatggat agaccgtttg aaatatattc tgttaccatc aattacccaa
     2641 gcatttttgg cgactgccgt cacaatccaa tatctgcgaa gtgaagtaat cgattcgaaa
     2701 tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac cgacgaataa agtattcagt
     2761 cgacatattt tccgaaatgc agcgttgccg atggcttctc aaatggggta tgaaatcact
     2821 tcgttgattg ctggttctgt agttatcgaa aagatttttg gttatccggg agtcggaaaa
     2881 ctatttattg attccatcgg ccaacgggac tataccgtga tcacatcctt agtattgatt
     2941 ttaggaatcg cgacgctagt ggggacatta ctttcagata tcatcatgag tatcgttgac
     3001 ccgcgtattc ggattcaata aaaccaatca gaaaggagaa tggatcgatg agtgaaaaaa
     3061 acaatacagc agttcaagaa agtattcctc ctatggggat acgaatgatt gctcgagaat
     3121 ttaaaaaaga taaaattgct attttttcat taggtttgct agtggtttta ttattagtga
     3181 tctttatcgg tgcgatgctg accgatcaag acaaagtgat gtatgttagt atcttcgaca
     3241 aatacgctgc accaggaaca gtttcgacac aaggacaaaa gttcattctg ggagcagatg
     3301 aaggcggacg tgatgtatta ggacaattga tcataggtgc tagaaactct gttacgatcg
     3361 gatttgcgat cacgatcctt acttcgatca tcggggttgg attagggatc atctctggct
     3421 actacggagg gatcattgac aacatcttga tgcggatcgt tgattttatc atgattctac
     3481 cgatcatgct gatcattatc gtcttcgtat cgattatttc cagatacaat gtctggtcct
     3541 ttatattcat catgagtgca ttttattggg tagctaaagc ccgattattc cgaagcaaga
     3601 cattatctga agcaagaaga gattatgtca gtgcctcaaa aacaatggga accaatgatt
     3661 ttaaaatcat gttccgcgaa atcatgccga atctaagttc attaatcatt actaatttga
     3721 cgatgaactt tgcggcaaac atcggtatcg agacaacttt gactttctta ggtttcggct
     3781 tgccggcaaa tgtacctagt ttaggtacat tgatcggtta tgcaagtaac ggagaagtct
     3841 tagtgaatcg gcaatggatc tggttacccg catcaatcct tattttagtt ttgatgttga
     3901 gtatcaatta tgttggacaa gcattcaagc gttctgcaga tgcgcgccaa cggttaggct
     3961 agtatagaag cgaaggggga aataggaatg aagaaaacga tttttggatt tgcagcgctg
     4021 atgtctgctg tcgttcttgc agcatgcggg ggcggaggta cagatacctc tggaaacaac
     4081 tctggaggca acggcaataa caaagaggaa ttaacttttc caattgcaac aaaaaataat
     4141 gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg caacggacag tcagtttaaa
     4201 ggattgttcc agtgggagtt ttaccaagat tcatatgatg cagcttttat ggctccttct
     4261 catgaaccgc tatttgcaaa cgatgacgat ttcagaatca cagacgacgg agctgcaacg
     4321 cttgatctag atgaagatac gaaaaaagcg acaatcaaag taaaagacaa cgtcaaatgg
     4381 tcagatggtg aagatgtaac ggcggacgat gtgattttcc cttacgaaat cattgggaat
     4441 aaagattaca ctggtatccg ttatgacgac actttccgta acatcgtagg tatggaagaa
     4501 tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa aaatagacga caaaacagtt
     4561 gaaatcgaat ataaagaagt aaacccaagc atgcttcaat cgggtggtgg tatctggtca
     4621 tatgcagcac caaaacatac gttagaaggt gtagcaatca aagatatgga atcaagcgat
     4681 caagtacgta aaaatccagt gacttttggc ccttattaca tgagtaatat cgttgcagga
     4741 gaatcagtag aatacttgcc aaacgagcac tactggaatg gtacgcctaa attgaaaaag
     4801 atcacgatga aatctgtacc gatggcaagt gcaactgaag cattgaactc taaattatat
     4861 gatatgattt tccaaatgcc aacagatact tacgatacct ataaagaaat tgctggctat
     4921 acaaacttag gccgtgaaca aacttcttat acttatctag gattcaagtt aggtaaatgg
     4981 aatgcagata caaagagtgt ggaatacaat ccagattcaa aaatggctga taaatcatta
     5041 cgtcaagcaa tgggctatgc attagataat aatgcagtag gtgaacgttt ctatgcaggt
     5101 ttgagaagca atgcgacttc attgattcct ccagtattcc agtcatacca tgatgatgag
     5161 atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac tattagatga tgctggctac
     5221 aaagatgtgg atgacgatgg tttaagagaa gacaaagatg gaaataaact aacaatcaac
     5281 tttgcttcga tgtccggtgg ggaaacagcg caaccattgg cagattacta tatccaacaa
     5341 tggaaagaaa ttggtttgga tgtccaatac acaacaggtc gtctgatcga attcaattct
     5401 ttctatgata aattagaaaa cgatgatcct gaaattgatg tttaccaagc tgcttggaat
     5461 acaggaactg atccaaatcc aagtggacta tggggaccaa atgctgcgtt caactacact
     5521 cgttatgaat cagaagaaaa tacaaaattg atcaacgata tcagttcaag caaagcattt
     5581 gatgaagagt accaaaaaga agcatatcaa aaatggcaac aatatgcatt tgaagaagca
     5641 tttgcgattc caacgttgtt ccgtaatgaa gtattgccag tcaacgaccg tgtcaaagac
     5701 tgggattggt cttatgatgt accaaaccca tgggcaaatg tttctgttac agcagattca
     5761 cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc ctttttcggt tttttgatat
     5821 ttttctgttt atttctctaa tattcaggtc aaggcgctga tttcatgact tctccaataa
     5881 acggtgttca aagtccagtt tctcggtatt aagtcaaatt tatgcaaaaa tatggaaagc
     5941 cattttcaca aaattttttc ttcatactcg aaactaaacg aatttttcac aacttctcgt
     6001 caatcactag gattattaaa gctttactta ccttttgacc gaaccatttc aaatttgaca
     6061 aaaaaccagt ataataagga agtatcgaaa aaatatccat aaaaggaatg tggaaaatgg
     6121 acaaccagct gacaaaagaa ctaaaagaaa actataacat cgtttttcac gatctaaatt
     6181 tgcttgaaca agcttttacc cattcatcct atgtgaatga gcatcgcaat ctgcaattat
     6241 cggataatga gcgacttgaa tttttaggag atgccgtttt agaactgatg gtttctcagt
     6301 atttattccg tttatatcct catttgccag aaggaaaatt gacaaaaaca cgagctgcga
     6361 tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg tcactttgat caatatgttt
     6421 tgttgggcaa gggagaagag aattcaggag gacgtacgcg accagcattg ctttgcgacc
     6481 tattcgaagc ttttttaggc gcgttatatc ttgaccaggg attcgacacg acccatgcat
     6541 tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc tttttcacat gagatggatc
     6601 ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga tgtatcaatc gaataccgat
     6661 tgatcaatga agaaggtcca gctcatgaac gagtattttg gatcgaagtt tatgtagatg
     6721 atcaattgat tggcactgga caagggaaat cgaaaaaatt agcagaacaa gctgcggcgg
     6781 aaaatgcatt ggcagcactg taaaagtaag tgagggaaag ctttgtattt gaaacgaatt
     6841 gaaatagccg gttttaaatc atttgcagac aagacagtca ttgattttga aaatagcgtc
     6901 acagcagtgg ttggaccaaa tggcagcgga aaaagcaata ttacagaggc tatccgctgg
     6961 gtattaggag aacaatctgc taaaagtctg cgaggcggaa aaatgccgga tatcatcttt
     7021 gccggttccg atacccgtaa gcagctgaat attgcagaag tcacagtgat tttagacaat
     7081 agtgatcatt atctgccatt ggagtacaat gagatcagtg taacccgccg ttatcgacgg
     7141 actggggaaa gcgagttttt catcaataaa caaagctgca gattaaaaga tatccaagaa
     7201 ttgttcttag attctggatt aggaaaagaa tcgttctcaa tcatttctca aggaaaagta
     7261 gaagcgattt tcagtagtaa accggaagac cgtcgtggga tctttgaaga agcagctggt
     7321 gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga agctttttga aacagaagac
     7381 aacctcagcc gcgtccaaga catcatccac gaattggaag aacagttaac tcctttggcc
     7441 gcccagagtg aggcagcaaa ggagttttta cggctaaaag aaacactgac tcaaaccgat
     7501 gtctctttga tggttgctga aatcaagaca gcaaaaaaag attgggacaa caaacaagca
     7561 cagcttgcga aattcaatct agaattaggc aaactatctg agtcgatcca agaacaagaa
     7621 tccattttgg cgaagcagcg taaagaaaat gcgcaagccg atcgtctgat cgaaaaaaat
     7681 cagcaagtat tattagatct ttcggaaaaa ctgaagcaga cagaaggaca aaaagatgtt
     7741 ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag aatatcaaac ttcattagca
     7801 gaagcacaaa aaaaggttaa acattttgaa aagcttcaag aaagcttgat gaaagcagca
     7861 gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc tcataaaaac acagcaagaa
     7921 ttagaaaaat accaaaaatc aacgaaagaa ttattggcag aattaagaga tcaatatgtt
     7981 gatttgatgc aagaacaagc agctgtagga aacgaactga agtatctaga acgtcaatat
     8041 atacaagaaa cagcaaaaag caagcagacg ttagccaagc aatcggaagt agaagcatca
     8101 gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa agcaggcgca gctaaaatca
     8161 tctttaactg aaacaaaaga gaagttagaa atgatccaac aaaacgggaa aaagttccaa
     8221 gaagcattag caaaagaaca gcctaaaatg tatcaattaa tgaatcaagt ccagcagcta
     8281 cgtgctcgta agaaaagcct tcaagaaatc caagaaaact attttggttt ttaccaaggg
     8341 gttcgattgg tcttgcagca taaacagcag ctttcgggta ttgtcggagc agtggctgaa
     8401 ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag cagctttagg tggggcagcc
     8461 cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag ccatcacgta tttgaaacag
     8521 caaagaggag gaagagcaac attccttcca ctgacgacaa tcaaaccaag acaattaccg
     8581 gctcatatat tgacacaagc agcagctgta gaagggttta tagggattgc cagtgaacaa
     8641 gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc tgctaggaac gatcctttta
     8701 gccaaagact taactagtgc caatgcaatc gcgcaaacga tccgctatca atatcgagtg
     8761 gtttccttag aaggtgatgt gatgaatgcc ggaggatcca tgacaggtgg agcgaataaa
     8821 cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat tgaaacagtt gactagtgaa
     8881 tttgaagaag cagataaaca actccaagca caagaaaaaa aagttcagga gctgcaacaa
     8941 gaaacagctc gtttagccga agaacaagaa gttttgcgta cacgaggcga acaattacgt
     9001 tttgaagaac aagaagcgac aaatcagcta caaaacatca taaatgaact tgaacgtttt
     9061 gaaaaagaaa agcaaatctc aacttttgag acacgtgaac tgcagcagtt tatcgaagat
     9121 tacgaaaagc aaacgaacga attgaaagac aaacaaacag atcttgaatc gcaacgacaa
     9181 aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg accagatgga agccagaaga
     9241 gcacaagtac aaagtcaaaa agcacaagaa caagcagatc ttgctgtctt gaaagagcag
     9301 tttaatcatc tgcagatcca gctccgcggt gcccgagtcc aaaaagcaga ggcaaccgaa
     9361 cgtcaagaag cgatagaaaa gcagttggcg acattgacag cagacttttc tgatcatgaa
     9421 gtcacagaag aaagtttgga aaaacaaatt aacgaattat ctgctcagcg agaaacatta
     9481 aaagccgagt tggcaaaagc caaagagcag cgagatagaa ggcaaaagga gatcgataag
     9541 cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag cacgactgtc ggaacaatcc
     9601 aaattagaag tccaaaaaga ccgagctgaa atgattttag ataatcattt gacctatctg
     9661 cagtcagaat atcagctgac atttgagaaa gcttcccaag actatcaaga aacaaccgat
     9721 atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac agattgaaaa actgggacca
     9781 gtaaacttaa attcaatcga acaatacgaa caagtcagtg aaagacatac attcttagca
     9841 acacagcgag atgatctgtt agcagcgaaa aaccaattgt ttgaaacgat ggatgagatg
     9901 gatgatgaag tgcggacaag attcaaagaa gtatttgaag ctatccgtca agaattcaaa
     9961 attgtctttc caaatatgtt tggcggcggt aaagctgaat tagtactgac ggaaccaact
    10021 gatttgctga aaacaggtat tgagattgaa gtacaacctc ctggcaaaaa actgcaaagt
    10081 ctaagtttgc tttctggagg agaacgagca cttacagcca tcgcgttgct cttttccatt
    10141 atccgtgtct gtcctgttcc gttttgtatc ttagatgagg tagaagcagc gttagatgaa
    10201 gccaacgtga aacgttttgg tcgttacttg agcgatttcc aagatgacac acaatttatc
    10261 gtagtcacac accgcaaagg aacgatggtc gcagcagatg tactgtacgg agtgacgatg
    10321 caggaatccg gtgtatcaaa aatcgtttct gtacggatgg aagatatcaa tgaagaggcg
    10381 aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa attagtagca atcgatttgg
    10441 acggtacatt attaaatgaa caaaagctga tttcagatga gaataaacaa gcattagcac
    10501 aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg acgcccctta gctgcgatgg
    10561 cgcattatct gcaagaatta ggactggtag atgaaggtga ctacagcatc actttcaacg
    10621 gcggtttggt acaaaaaaat gatactggtg aaatcatgga aaagaaagtg atggaagtat
    10681 cagatatcca acgcttatac aaactggctc agcaattgga tctgccacta gatgttttgt
    10741 ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa gcaatccttg tacaatgtgt
    10801 tgaataagct gctgcaattc caaccagcgg atataagcga tattacagat gaatttgtgc
    10861 ttaataaggc agtaatcgct tatgaccaag aagaattaga tcctaaaatc aaagaaatac
    10921 cagcagaatt ccatgaaatg tatgagatca tcaaaacaag aagtatgctt ttggaattta
    10981 tgccaaaagg tgttacaaaa gcttatggta tttctttatt ggcaaaagat cttggtttga
    11041 aacaagaaga aatcatggcg atcggtgatg aggaaaatga tttaccgatg atccaatatg
    11101 ctggactagg cgtagcgatg gagaatgctg tgcctttcgt caaagaagca gcagatttcg
    11161 tgacagccag caatgaagaa aatggcgtag ccaaagtgat ccaaaaattt gtattagagt
    11221 agggggagtc aaatgggatt ttttgataaa atcaagcgtg cttttactgg cgaagagaaa
    11281 acagaagaag ttgaagtaca ggagaagtac gacaagggtt tagaaaagac gcgtaaaaca
    11341 tttggtgaac ggatgaatga gttgtttgcc aattttcgaa cagtcgatga agactttttt
    11401 gaagaattag aagaaacact tatcggtgca gatgtaggtt ttgaaacagc gatacgaatc
    11461 accgaagcat tacgtcaaga agtgaaactc agaaatgcga aaaaatcggc agaagtccaa
    11521 aatgcgatca tcgagaaact tgtggatctc tatgaagcgg aaggaatcaa tgaagtaaat
    11581 gaactaaata tccaagaaga cggactaaca gttattttgt ttgtcggtgt taatggtgta
    11641 gggaaaacaa caagtattgg taaacttgcc catcagttca aaaacgaagg taaaaaagta
    11701 ttgatggcag cagcagatac cttccgcgct ggagcaatcg accagttagt tgtctggggc
    11761 gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag gcgatccagc agctgtcgta
    11821 ttcgatgctt tggaaagagc aaagacagag caggcagatg ttttactagt tgatactgca
    11881 ggccgactgc aaaataaagt gaacttgatg aacgaattag aaaaaataaa acgagtaatc
    11941 cagcgggaat taccagaagc accccaagaa acacttcttg tattagatgc aaccactggt
    12001 caaaacgcga tggttcaagc aaaacaattc aaagaaacaa cagatgtgac cggtcttgtt
    12061 ttgacaaaat tagatggtac agcaaagggc ggtatcgtac ttgctatccg taatgaattg
    12121 catttaccag tgaaattggt cggtcttggt gaaggaatcg atgacttgga gccgtttgat
    12181 ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa aagaagaata acccatgtct
    12241 aaaaagcagg agctgtgaca aaagttgcag ctccttgttt gaatgattgt ttttaacata
    12301 gctgtgtttt tgatgccttc atttttatac ttatttgtaa gcggttctca aacaatttcg
    12361 ttctttttct tagacttttt gattataatt agattcatat gaattggagg tcacttaatg
    12421 atgaatttta agcaattatt attgcatgtc aatgcgcgcc cttttatcga ccaagctcga
    12481 tttggtatag aacgggaagg acagcgggtt gatcttgcag gaaatctagc aaaaaccgat
    12541 catccagcaa tctttggcga tcgatcctat cacccctata tccaaacaga ttttagcgaa
    12601 acacaaacag agatgatcac ccctgttacc gattctattc ccgaattatt tcagtatctc
    12661 gctgctgttt atgatgtgac tgctcgttct ataccgaaag aagagatgat ctggccatta
    12721 agtatgccac ctgccttacc ggaaaaagac gaagagatca ttattgcaaa attaaaaaat
    12781 ttcgaagatg tcttgtatcg acgttattta gcaaaagaat acgggaagcg aaaacagatg
    12841 gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt tgctaagaac attgttcagc
    12901 catcaggaag aatttcaaga tttttccgaa ttcaaaacag aactttattt gaaaacagcg
    12961 agaaacttta tgcgctatcg ctggatgatc acttatttat tcggtgcttc accaatgagt
    13021 gagaagaact actttttaga tgaatcacat ccgcaagaac ctgttcgcag tattcgcaac
    13081 agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt cctatgcttc tatgaaacag
    13141 tatttagcag atatcgagcg aatgatcgaa gaagggaaac tttcggagga gaaagaattt
    13201 tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag atttagcaac aacaggtgtt
    13261 cgttatattg aattgcggaa tatcgatttg aatccttatg caagattggg tatcaatcca
    13321 gagcaagttc ggttcttaca attattcctg atgtacatgt tatggacaga agaaaaagaa
    13381 gactgtgacc aatgggtagc agaaggtacg actcgaaata acaaagtagc actagaacaa
    13441 ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa ttcttgaagg aatgaagcaa
    13501 atgctggttg aattggattg gttggattct ctttatttag tagaggaagc attgactcaa
    13561 atggatcatc ctgaacaaac gttagcagcg aaactctatc aagaagcaca gctatcgagt
    13621 cagcaagaag ttgctgtggc attgggacat caatattata aagaaagcca tgaacgtccc
    13681 tatcagttgg ccggttttcg cgaaatggaa ctttctactc agatatttat gttcgatgcc
    13741 atccaaaaag gtgtccaagt caaagtatta gatgagtcgg atcaattttt gcgtctgcaa
    13801 ttccaagacc atgtagaata tgtgaaaaat gcaaacatga caagtaagga cagctatatc
    13861 gtcccactta tcatggaaaa caaaacagta acgaaaaaag tcttaaaaga agctgggttc
    13921 cgggtaccag gaggcgcaga attttcatcc atggaagaag cggtaaaagc ttatccgaga
    13981 tttgccgagc aggcatttgt gatcaaacct aaatcaacca attacgggtt aggtatcacg
    14041 attttcaagg aaggagctag tctggaagat tatcaagcag gtttagccat tgcttttcgt
    14101 gaagacagtt cggttttagt agaagaattt atgccgggaa cggaatatcg tttctttgtg
    14161 atcgacggag aagtccaagc catcatgttg cgagtccctg ctaatgttat tggtgacagt
    14221 atccgaacag tgaaagaact tgttgaagaa aaaaatagtg accctttgcg tggaacgaat
    14281 catcgtgcac ctttagaatt gatccaatta ggtgaattag aacaattgat gttgaaagaa
    14341 caagggttga cgatcgaatc agttccccaa gccaatcaaa ttgtttatct aagggagaac
    14401 tcaaatatca gtacaggtgg tgattcgatc gatatgactg atgaattttc tgaagcttac
    14461 aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta agattagtgg tatcgatttg
    14521 attataccag ataaagaaat cgatcctaca actgataaaa aagcatatgg gatcatcgaa
    14581 gcaaatttca atccagccat gcatatgcat gtttatcctt ttgcaggaaa aggcagaaga
    14641 ttgacgatga atgtattgaa attgttatat cctgaagtat tttaaaagaa aaagcttttg
    14701 acagattgtc ctatttgatg tcaatctgat caaaagcttt ttttgcatgt aaaaatttat
    14761 gtgttttttc gttttaaaat aaacgatccc agaagctttt tttctttttt ggtttttctt
    14821 tttcagctgg cgaagagttt tcttctgggt attttttttc gatatcaatc acaggttcat
    14881 ttactgcttc ttttgctgtt aagagcaatc cgaattcctc aggatttgca gtgacgtaat
    14941 cgttgactac agtgaaacgg ctattgtttt tcgttgctag ctggatatat tggttttgga
    15001 cagaaagggg tagagcccca ttcaagagta tggaagcatc tggatgtttt ttgagttctt
    15061 ttaaaaagtt ttctttgttt tttggttgga tcatttcagc aacagtcata ctaagatagc
    15121 agcgttccct gaatgtccct aaatatttat gctgttcttc agggttcact aaaggtgtac
    15181 cataaagtcc cttatccaaa tggcttttta catcatcttg tgccataaaa aataccctcc
    15241 ttcttctctt tgtctttctt acgattatag catattccca ataataccta aaacatatga
    15301 aaaagcggtt gcttttgttc aacgattcac ttaaaataag aatgaaaatc attctcaatt
    15361 agaaaggtgg attggatgcg ctacggacga ctagtagcat tattagttat tttaagtagt
    15421 atttcattgt tcatcggagt atcgaatgta caattgtcag aactgctcca gttagatggg
    15481 caaagcttac aaatcttgct agtgactcgt ttgccacgaa cgatttgttt gatccttgtg
    15541 ggagcgacca gtagtatttg tgggttgatc atgcaacatt tgacccaaaa taaatttgtt
    15601 tcaccaacga ctgcgggcac gatggatagt gcgcgtttag gtattttagt ggctatgatt
    15661 ttcctacctg gtgcatcctt gattgtccga tccttgacag cattttgctt tgcttttgca
    15721 ggaacattgc tttttttatc ccttacgcga ttgttcctcc agaaaaatca attgatgctt
    15781 ccgttgattg gagtcatgtt agggaatatc attggttcag ttgctacttt ttttgcttat
    15841 caattccagt tggtacaaaa tatgacttca tggcttcaag ggaattttgc gactgtgata
    15901 cgaggaaatt atgagttatt gtatgctgtt gttccattgt tgctcttggt ttggctattt
    15961 gcttaccgat ttacgatcat tggtctgggc gaatcgtttg cagtaaatct gggaatagat
    16021 taccagaaga tgcagttttt aggcattggc ttagtatcat tggctagtgc agtcatgttg
    16081 atcatggtag gaagcattcc ttttatcgga gtgatcattc cgaatcttgt ctctagaata
    16141 tatggagatc aagtgcacaa aacaattggc gtaactgctt tagcaggtag tttgttctta
    16201 gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt atgaaatacc agtcagtgtg
    16261 atcgttggaa tcatcggtgg ggctatcttc ctttatcttc tgataagggg acgacgatat
    16321 gcttaaaaaa aagccatcta tttttattgg tatgcttgtt cttgttttat ttgttacagg
    16381 aatgtacttg acgatccata catatggaaa ctggcatttt gcatgtgcat tgaggagtaa
    16441 gaaaattttt gcatttatcc ttgtctcgct agctgtcagc agtgccacaa tcagctttca
    16501 gacaattacg cataaccagt ttttgactcc ttcagtattg ggacttgagt cgttatatgt
    16561 ccttgtccaa acggtccttt tcttctttat cggaggagtg acgatgcttc agcagacagg
    16621 tacagttttt ttcttattga acgttgggct gatggtcggg ctgagtctat tgttattttt
    16681 ctttcttttg aaaaaaatga gagcgaatct ttttttatta ctgatgtcag ggatgatttt
    16741 aggtacgctt tttggtagtg tcagtacctt tttacaagtc atcatggatc cgaatgaata
    16801 tgatttgctt caaggcagat tatttgcaag ctttggcaat atccagagtc agtatctttt
    16861 gcctgcttct ttgatcatta tcggctgctg gtacctgtta tttcgatatc gacatgtgct
    16921 agacgttttc catctcggaa atgaacaagc agtaaacctc gggatagaca cagatcgttt
    16981 tgaatttttg attttatgtt gtgtcagtct tttgacaggg acatcaacgg ctcttgtggg
    17041 tccagttact tttttagggt ttattgtcgc aaatatcagt tatcgtgtct tttctactta
    17101 tcgtcatggt cagctattta tcggaagctt tctggtagct atgctgtttc tgacaggagg
    17161 acagctcgca gtagagcaga ttttcggctg gaatacaaca gtgagtgtcg tcgttgaatt
    17221 tgtcggagga atttattttg tcacaaaatt gattcgcgaa aggaagagta tcctatgatt
    17281 gatttacaaa aggtcaataa actgtaccat ggaaaatcag tggttaaaga tgtacagtta
    17341 acgattcctg aaaagaagtt gactgctttt atcggtccaa atggtgctgg aaagtcaacg
    17401 gtgttgtcga tgatcagtcg tttgatacca aaagatacag gtgaaatcta tttagaccat
    17461 aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa aattatcgat cctacgacaa
    17521 tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag tagaatttgg acgatttcct
    17581 tatacgaaag gaaaattgac gacaaaagac gaggaaatcg ttcaggaatc actggaacac
    17641 attgggttag aagaaatagc ggatcaatcg attgacactc tttcaggcgg tcaactccaa
    17701 cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt atattttact ggatgaacct
    17761 ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc agattttaaa aaatttagta
    17821 caagaaatcg ggaaaacaat tgttattgtt cttcatgata tcaactttgc tgcaagttat
    17881 gcagacgaaa ttgttgcaat gaaagatgga cagttattta cgcaaggaac gacccaagaa
    17941 atgataaaaa aagatgtatt ggatgaatta tacgaaatgg acatacggat ttgtgagatt
    18001 gaaggccgca gattctgtct ttatttctaa aactaacatt aggagagaag agatgaaaaa
    18061 attagtagta ggtatcttga ttctagcatc atttggttta gcagcatgtg gaaatagcaa
    18121 caacaccagt caggcagaca caaaaaaaag cagcacacaa actgaaacta cattgacgat
    18181 aacagatagt aatggagacc aaatcgaggt gccaaataat ccagaaaaag tcgtcgtatt
    18241 cgacaatggt tctttagata cgatggatgc tcttggtgtt ggagataagg tagttggagc
    18301 agctacaagc agccttcctg agtatctttc atcctataaa aaagtagaat cagcaggtgg
    18361 aataaaagaa ccagacttag aaaaaattaa tcagcttcag ccagatctaa tcatcatttc
    18421 tggccgacaa agagattttc aaaaagatct aagtcaaatt gctccgacca ttttcctttc
    18481 tttagatgcg aaaaatccat gggaatcttt ccagcaaaat gtcactgctt taggggagat
    18541 ctttggtaag caagaagaag ctaaaactca attagaggaa ttatctagtg caatcgatca
    18601 gacgaaaaag aaagcagaag ctactgacaa aaaagcacta gtcacacttg tgaatgaagg
    18661 gcaattatca gcctacggat ctggttctag attcggtttc atccatgatt tatttggctt
    18721 tgaacaagca gatgatcaaa tcgaagcatc cactcacggt caaagtgttt cttatgaata
    18781 tgtgctggaa aaaaatccgg atatcttatt tgtagtagac agaacaaaag ctattggtgg
    18841 agacgacagc aaagatgata tttctgccaa tgaactggta gcacaaacga atgctggtaa
    18901 aaatcagcag atcatctccc ttgaaccaga tgtctggtat ttaagcgggg gcggtttaga
    18961 atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt aaatagcata aataacagag
    19021 gattaaactc tcatactatg taacaataat gtttcttaca ttcttaaata gcaagagcct
    19081 atcctcaaag aagttatcaa aaaccatccg taaaatcgta ttgatgattt tacggatggt
    19141 ttttatttat ttgagaaatc ttttttcaac gtgaattcat tatctacatc atacgggttg
    19201 attctagaat ggggagtggc aaaaaatagt gtgatccatt ttttcagcat caagatcgcc
    19261 tcctttgatt tgaatcaaag gtacaaagcg aaacttagat aaaacttaaa tttagatatt
    19321 tttattttgt accgatctct tctttaaacg atgttccatc tgctgcttct cggcaataag
    19381 tccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat tactcaaaac
    19441 aaatcgcagt tgtcacaatc tcttctaaac gatgttccat ctgctgcttc tcggcaataa
    19501 gcccactaga ctagaaaata tgagaagcta ttttctgtcc agcgttctta ttactcaaag
    19561 caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa tcagctgacc ttcggtatta
    19621 gctcaaagca ctgatttcac aacctctatt taggcatgtg tctcagcaag cggttgatag
    19681 ttgatgatct gcatttttcg ataaaaatcg gcgggaagct tactccaccg ttttagtaat
    19741 tgatagaaat aactctcttc tgctttgatt ttatactcgc ttaatatcaa tccaaaacta
    19801 ttcgtttttt ttgaagctaa ttttttataa atatccaatg ctttggtttt atcttcattg
    19861 acaaagagtg tttttacttc aatataagaa ccctcgatat tttgtgtttc ttcgataaaa
    19921 tagatgtttt ttatatattc cttcatagtg attcctccaa agaagtcttt tatttgataa
    19981 gtttactcta ccgaagagtt gttaataatc cgtgaacaaa aagtaattct ttttccaata
    20041 gttgttaaag gttttttatt ttttatagta ttttttgtta tttaagaaag gaataaggtt
    20101 aaacaaataa caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag
    20161 ggaaagttgt ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat
    20221 aaattgagaa cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc
    20281 tatgacatga ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga
    20341 agaataaatt agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat
    20401 ttgcagaaag ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa
    20461 aagatacaca aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa
    20521 tcaaaatcaa agacagtgca atactttaca tggatgatcc atccacactt gctttagaac
    20581 caacttataa tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag
    20641 ttgttcgaaa agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg
    20701 tggacatcaa taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc
    20761 acaagatcac tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag
    20821 ctgttgtcga atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg
    20881 agttggcaga tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa
    20941 atgaacggta tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg
    21001 actatcagtt ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt
    21061 ggtatgatcg gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg
    21121 cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca
    21181 ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga
    21241 acaaattaaa tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa
    21301 atatcaaaca agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc
    21361 acacgattta cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt
    21421 ttgtcttgaa agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc
    21481 gtgcccatca cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac
    21541 aagcgatgac aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt
    21601 gtaaaacata aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt
    21661 tgcggaaaat aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta
    21721 gatgcaagcg tgtattcttt ttttgaacga taatcacaaa aaaaaatata atatacattt
    21781 tttatttaat tataattaat attgattgaa gtctaggcag tcaagtcgag agtgtaaaat
    21841 attttgtgta aatgaaaaaa tccatacaaa aaaggaagtc
//
LOCUS       DAZKOK010000041        21743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000041 DAZKOK010000000
VERSION     DAZKOK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.041"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1387
                     /locus_tag="ACG90W_001340"
     CDS             47..1387
                     /locus_tag="ACG90W_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0477715.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1605..2048
                     /locus_tag="ACG90W_001341"
     CDS             1605..2048
                     /locus_tag="ACG90W_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0477716.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACG90W_001342"
     CDS             complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACG90W_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0477717.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4373..5596)
                     /locus_tag="ACG90W_001343"
     CDS             complement(4373..5596)
                     /locus_tag="ACG90W_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0477718.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5627..6466)
                     /locus_tag="ACG90W_001344"
     CDS             complement(5627..6466)
                     /locus_tag="ACG90W_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0477719.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6483..7163)
                     /locus_tag="ACG90W_001345"
     CDS             complement(6483..7163)
                     /locus_tag="ACG90W_001345"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0477720.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7165..8238)
                     /locus_tag="ACG90W_001346"
     CDS             complement(7165..8238)
                     /locus_tag="ACG90W_001346"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477721.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8294..8532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8575..8808)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8885..9556)
                     /locus_tag="ACG90W_001347"
     CDS             complement(8885..9556)
                     /locus_tag="ACG90W_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0477722.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9643..12291)
                     /gene="mgtA"
                     /locus_tag="ACG90W_001348"
     CDS             complement(9643..12291)
                     /gene="mgtA"
                     /locus_tag="ACG90W_001348"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0477723.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12633..12807)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12980..13417
                     /gene="ndk"
                     /locus_tag="ACG90W_001349"
     CDS             12980..13417
                     /gene="ndk"
                     /locus_tag="ACG90W_001349"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0477724.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13439..14305)
                     /locus_tag="ACG90W_001350"
     CDS             complement(13439..14305)
                     /locus_tag="ACG90W_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0477725.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14465..16558)
                     /locus_tag="ACG90W_001351"
     CDS             complement(14465..16558)
                     /locus_tag="ACG90W_001351"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0477726.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17307..17930)
                     /locus_tag="ACG90W_001352"
     CDS             complement(17307..17930)
                     /locus_tag="ACG90W_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477727.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18089..19486)
                     /locus_tag="ACG90W_001353"
     CDS             complement(18089..19486)
                     /locus_tag="ACG90W_001353"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0477728.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19518..20489)
                     /locus_tag="ACG90W_001354"
     CDS             complement(19518..20489)
                     /locus_tag="ACG90W_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0477729.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20863..21288
                     /locus_tag="ACG90W_001355"
     CDS             20863..21288
                     /locus_tag="ACG90W_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477730.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         6269 a         4496 c         3772 g         7206 t
ORIGIN      
        1 tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa
       61 attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg
      121 tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat
      181 tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt
      241 tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc
      301 tcattctgct gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc
      361 tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt
      421 atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt
      481 cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat
      541 ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca
      601 gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt
      661 gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc
      721 agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc
      781 cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg
      841 cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga
      901 tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc
      961 atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc
     1021 tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta
     1081 tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc
     1141 cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc
     1201 actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg
     1261 attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct
     1321 gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa
     1381 tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga
     1441 aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc
     1501 tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca
     1561 tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga
     1621 agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg
     1681 tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg
     1741 gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga
     1801 taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac
     1861 gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta
     1921 tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg
     1981 aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa
     2041 tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca
     2101 agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc
     2161 tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag
     2221 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg
     2281 ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt
     2341 tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc
     2401 gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat
     2461 ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac
     2521 aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta
     2581 ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca
     2641 tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa
     2701 taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta
     2761 ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat
     2821 aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa
     2881 tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct
     2941 ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta
     3001 atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc
     3061 taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc
     3121 cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag
     3181 cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta
     3241 aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca
     3301 cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa
     3361 agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca
     3421 aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat
     3481 ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg
     3541 ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta
     3601 cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga
     3661 tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa
     3721 tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga
     3781 tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca
     3841 ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg
     3901 caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt
     3961 tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata
     4021 tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt
     4081 tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc
     4141 actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat
     4201 cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt
     4261 tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt
     4321 tcatttttct gaaaattatt tttataactt tcacacacaa gattattttt tactagaaag
     4381 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta
     4441 atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg
     4501 caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg
     4561 caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat
     4621 ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa
     4681 cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat
     4741 catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac
     4801 ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac
     4861 gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg
     4921 taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat
     4981 taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
     5041 gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt
     5101 tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt
     5161 ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat
     5221 atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata
     5281 cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga
     5341 attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg
     5401 ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa
     5461 tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta
     5521 gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg
     5581 agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga
     5641 atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt
     5701 cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc
     5761 ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg
     5821 catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt
     5881 ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt
     5941 ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga
     6001 tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga
     6061 ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc
     6121 ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt
     6181 aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag
     6241 ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt
     6301 tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta
     6361 cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa
     6421 taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac
     6481 tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag
     6541 attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc
     6601 gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg
     6661 atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa
     6721 tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc
     6781 ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca
     6841 aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta
     6901 gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc
     6961 ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact
     7021 aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata
     7081 tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa
     7141 tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc
     7201 taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc
     7261 ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc
     7321 ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag
     7381 atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt
     7441 attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag
     7501 atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg
     7561 gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat
     7621 ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt
     7681 caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc
     7741 ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg
     7801 tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag
     7861 ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa
     7921 agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat
     7981 cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt
     8041 ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa
     8101 cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt
     8161 taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac
     8221 atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg
     8281 aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt
     8341 tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc
     8401 atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
     8461 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
     8521 caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag
     8581 gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
     8641 aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg
     8701 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga
     8761 gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt
     8821 gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct
     8881 atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt
     8941 ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat
     9001 caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat
     9061 cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag
     9121 gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat
     9181 cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa
     9241 tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta
     9301 agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt
     9361 cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt
     9421 tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc
     9481 cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa
     9541 atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg
     9601 actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa
     9661 tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc
     9721 aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact
     9781 tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa
     9841 tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt
     9901 ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg
     9961 ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata
    10021 catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa
    10081 ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg
    10141 atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa
    10201 aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc
    10261 aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc
    10321 agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga
    10381 ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc
    10441 aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc
    10501 tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc
    10561 tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt
    10621 gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag
    10681 aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt
    10741 tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga
    10801 aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc
    10861 ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt
    10921 aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcaccttt
    10981 tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc
    11041 aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc
    11101 tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct
    11161 atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata
    11221 ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat
    11281 tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac
    11341 acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc
    11401 aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt
    11461 gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt
    11521 atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg
    11581 accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac
    11641 attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca
    11701 tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa
    11761 taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg
    11821 aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt
    11881 tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac
    11941 ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc
    12001 gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc
    12061 taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa
    12121 tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat
    12181 ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt
    12241 ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc
    12301 tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat
    12361 cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc
    12421 tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat
    12481 tttaattcat gacttccaat catttttcga atagttattt ttccaattag accacctcct
    12541 ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc
    12601 gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca
    12661 ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga
    12721 caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta
    12781 taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa
    12841 gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat
    12901 tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag
    12961 taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag
    13021 acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga
    13081 aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga
    13141 gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt
    13201 gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc
    13261 cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat
    13321 tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt
    13381 cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct
    13441 agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc
    13501 ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta
    13561 aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac
    13621 ttctttcttt atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca
    13681 accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct
    13741 catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct
    13801 caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac
    13861 catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt
    13921 caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat
    13981 acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa
    14041 agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat
    14101 ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg
    14161 atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag
    14221 tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt
    14281 tcttgatcaa ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat
    14341 tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt
    14401 cataaaaaat accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga
    14461 ttgtttattg attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg
    14521 ttgttgtttt attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt
    14581 cgattgcttc attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct
    14641 caaatccgtt tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac
    14701 ttaacgcagt cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt
    14761 tcgtatcgat gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt
    14821 tgggtgtcac aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata
    14881 agtctgtacc aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg
    14941 gcaaaacatc gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca
    15001 tatggatcat caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg
    15061 gatctttacc aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat
    15121 ccccgtaaat cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa
    15181 attgttccaa tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat
    15241 tgccagtatt caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt
    15301 tggttacgga aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag
    15361 attctctgaa taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa
    15421 aataagatcg atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc
    15481 aaaaactagc tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga
    15541 tcgctggtgc ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag
    15601 gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta
    15661 cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa
    15721 acggtgtgac ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta
    15781 aatggagaac gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg
    15841 cataatgttt ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc
    15901 gaacactgct agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta
    15961 gatatttgac aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt
    16021 cgctcagcgc tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca
    16081 tcggtttgcc gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta
    16141 gcccaatgaa cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa
    16201 aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa
    16261 tgaaatctgt gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa
    16321 tatcaatgat caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc
    16381 caaaaaacaa taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac
    16441 cttccacacc taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga
    16501 aaacagctgt caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt
    16561 atatcacttc tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct
    16621 aaaaaagttg ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc
    16681 acagcataaa accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg
    16741 tgaccgcttc actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag
    16801 gctgtgacaa aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa
    16861 cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa
    16921 aacatgagaa gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca
    16981 agacctcttg atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg
    17041 ctaattttac aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    17101 aaccaaacga aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa
    17161 acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct
    17221 caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa
    17281 aacgctgatt tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat
    17341 gactcattcg taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg
    17401 ccagaatctg aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc
    17461 ttgacttgtt catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct
    17521 actttttcag aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca
    17581 tcatgagtca aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa
    17641 atcgcactaa gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa
    17701 taaatgtcca attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa
    17761 gttgagaatt cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt
    17821 tctttctgag caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt
    17881 aagaaaaaac cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc
    17941 actcctattt aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa
    18001 gttgaaaaag atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt
    18061 tcacgcattt gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt
    18121 ctttgaacca atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg
    18181 aaataaagcc atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc
    18241 acatatgata gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat
    18301 gagattggac aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc
    18361 gtgcttcgtt ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat
    18421 gatctcgtac attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat
    18481 aaggattcat tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac
    18541 cgtttttagt atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga
    18601 tacctaaaag atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag
    18661 gcatgagatc aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag
    18721 ctggatcaag gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt
    18781 cagggtttga ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac
    18841 caccttgctc catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat
    18901 agccaacttg gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat
    18961 aacctgcttc gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac
    19021 caaacaattc aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca
    19081 accatccgcc tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg
    19141 gttcgatacc gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt
    19201 cgttgattgt tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg
    19261 aattatgccc agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg
    19321 aggttttttc aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat
    19381 caaaaatgtt ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg
    19441 ctgcagaacc ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt
    19501 ttctttttta gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt
    19561 tcattttctt cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg
    19621 attttatctg ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct
    19681 aaataatcat agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa
    19741 cattcgatgg caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt
    19801 tctaataatg cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc
    19861 tctaattgga tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt
    19921 gctacgtagt ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca
    19981 tgatgagcga tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct
    20041 gctggtcctt ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca
    20101 aatccagacc actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc
    20161 aggtattcaa ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag
    20221 ctatacccat tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg
    20281 ctggaaatcc cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta
    20341 gcaatgatgt tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc
    20401 ttttccttca gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc
    20461 atcttagcca catcacggat cgatgccata cattctcact cctgccagtt acatttgatt
    20521 tctctttctt gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca
    20581 atatccatat aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa
    20641 acaagtattt gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt
    20701 tttaaaacgc tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt
    20761 tactctattt gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc
    20821 caatggtata atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta
    20881 ctatttattt tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct
    20941 gaccaatcct cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc
    21001 accaacagtg tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg
    21061 acatttacag ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct
    21121 tctgaatcat ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac
    21181 gaatatatta tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc
    21241 ggtaattacc gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg
    21301 gatcttcttt tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa
    21361 ggagacgaaa tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat
    21421 ccatacagaa tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt
    21481 caaagattca tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag
    21541 aagataaaca aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc
    21601 ttcttccttt gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat
    21661 caaagagtaa agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa
    21721 ggcttattct acatcacgta gta
//
LOCUS       DAZKOK010000042        21606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000042 DAZKOK010000000
VERSION     DAZKOK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.042"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACG90W_001356"
     CDS             complement(41..1432)
                     /locus_tag="ACG90W_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477731.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACG90W_001357"
     CDS             1689..5078
                     /locus_tag="ACG90W_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0477732.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACG90W_001358"
     CDS             5081..6502
                     /locus_tag="ACG90W_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0477733.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACG90W_001359"
     CDS             6499..8376
                     /locus_tag="ACG90W_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0477734.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACG90W_001360"
     CDS             8468..9310
                     /locus_tag="ACG90W_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0477735.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACG90W_001361"
     CDS             complement(9462..10337)
                     /locus_tag="ACG90W_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0477736.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACG90W_001362"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACG90W_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACG90W_001363"
     CDS             11663..11968
                     /locus_tag="ACG90W_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0477737.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACG90W_001364"
     CDS             12147..14162
                     /locus_tag="ACG90W_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0477738.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACG90W_001365"
     CDS             14164..14448
                     /locus_tag="ACG90W_001365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0477739.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACG90W_001366"
     CDS             14474..15847
                     /locus_tag="ACG90W_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0477740.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACG90W_001367"
     CDS             complement(15930..17945)
                     /locus_tag="ACG90W_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0477741.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACG90W_001368"
     CDS             complement(17967..19181)
                     /locus_tag="ACG90W_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0477742.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..20345
                     /locus_tag="ACG90W_001369"
     CDS             19401..20345
                     /locus_tag="ACG90W_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0477743.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20641..21585
                     /locus_tag="ACG90W_001370"
     CDS             20641..21585
                     /locus_tag="ACG90W_001370"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0477744.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7333 a         3961 c         4085 g         6227 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg
    20161 atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc
    20221 aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt
    20281 ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac
    20341 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
    20401 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
    20461 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
    20521 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
    20581 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
    20641 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
    20701 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
    20761 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
    20821 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
    20881 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
    20941 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
    21001 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
    21061 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
    21121 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
    21181 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
    21241 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
    21301 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
    21361 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
    21421 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
    21481 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
    21541 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
    21601 aactaa
//
LOCUS       DAZKOK010000043        21292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000043 DAZKOK010000000
VERSION     DAZKOK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.043"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..710)
                     /locus_tag="ACG90W_001371"
     CDS             complement(99..710)
                     /locus_tag="ACG90W_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0477745.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(833..958)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1040..1756)
                     /locus_tag="ACG90W_001372"
     CDS             complement(1040..1756)
                     /locus_tag="ACG90W_001372"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0477746.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1762..2331)
                     /gene="scpB"
                     /locus_tag="ACG90W_001373"
     CDS             complement(1762..2331)
                     /gene="scpB"
                     /locus_tag="ACG90W_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0477747.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2315..3085)
                     /locus_tag="ACG90W_001374"
     CDS             complement(2315..3085)
                     /locus_tag="ACG90W_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0477748.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3082..3453)
                     /locus_tag="ACG90W_001375"
     CDS             complement(3082..3453)
                     /locus_tag="ACG90W_001375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477749.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3472..4359)
                     /gene="xerD"
                     /locus_tag="ACG90W_001376"
     CDS             complement(3472..4359)
                     /gene="xerD"
                     /locus_tag="ACG90W_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0477750.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4455..4928)
                     /locus_tag="ACG90W_001377"
     CDS             complement(4455..4928)
                     /locus_tag="ACG90W_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0477751.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5033..5896)
                     /locus_tag="ACG90W_001378"
     CDS             complement(5033..5896)
                     /locus_tag="ACG90W_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0477752.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6092..8191)
                     /locus_tag="ACG90W_001379"
     CDS             complement(6092..8191)
                     /locus_tag="ACG90W_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0477753.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8411..9520)
                     /gene="rpoD"
                     /locus_tag="ACG90W_001380"
     CDS             complement(8411..9520)
                     /gene="rpoD"
                     /locus_tag="ACG90W_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0477754.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9544..11385)
                     /gene="dnaG"
                     /locus_tag="ACG90W_001381"
     CDS             complement(9544..11385)
                     /gene="dnaG"
                     /locus_tag="ACG90W_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0477755.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11567..13882)
                     /locus_tag="ACG90W_001382"
     CDS             complement(11567..13882)
                     /locus_tag="ACG90W_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0477756.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14067..14927)
                     /locus_tag="ACG90W_001383"
     CDS             complement(14067..14927)
                     /locus_tag="ACG90W_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0477757.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14986..15618)
                     /locus_tag="ACG90W_001384"
                     /pseudo
     CDS             complement(14986..15618)
                     /locus_tag="ACG90W_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            15785..16546
                     /locus_tag="ACG90W_001385"
     CDS             15785..16546
                     /locus_tag="ACG90W_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0477758.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16572..19163
                     /gene="mprF"
                     /locus_tag="ACG90W_001386"
     CDS             16572..19163
                     /gene="mprF"
                     /locus_tag="ACG90W_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0477759.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19351..20538)
                     /gene="tuf"
                     /locus_tag="ACG90W_001387"
     CDS             complement(19351..20538)
                     /gene="tuf"
                     /locus_tag="ACG90W_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0477760.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20906..21262
                     /locus_tag="ACG90W_001388"
     CDS             20906..21262
                     /locus_tag="ACG90W_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0477761.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         5840 a         4467 c         3724 g         7261 t
ORIGIN      
        1 aaatactcaa aacaaacaaa taaaacgttt acattattgc atgtcttgta tttttttcca
       61 aaaaaaaaga ccaagaaatt tcttgatctt tttgatattt atttgactgc tgtatgacgt
      121 tgttcgaact gatgttgttt tcgacttaac catggaagaa gttgcgcatg cagcactaaa
      181 aagacagcac tcacgatgaa tcctttgatc aagttgaaag gaacgatccc aatcagtaca
      241 tagtttgcta aagacatgcc cagcatctgt gttgcgttca gccctaaaaa cattaagtaa
      301 agaggcgtaa taacgaaata atttgcgata ctcataaaaa tagttagagc gatggttcct
      361 gtaatgacac ctaaaaattt gtttcttcct ttatgctgtt tctttacact aaagaaataa
      421 taaatcggga atgtaaagat cgttgttgat aagaaactag cagtgtctcc taccaaatta
      481 tccggagaaa aacctgtagt cagcagatgt aatactgaac gcaagaacgc tgttgcaact
      541 ccggccgctg gaccgaataa gaacatgctg atcaataccg agatatcact aaaatcgatc
      601 ttcaaaaaac taaaagccgg catgatcgga aaagcaaaat actgtaaaac taatgcgatc
      661 gcagcaaaca tcgctactgc aaccattttc tgtacacggg tgttcttcat ttgaaatcct
      721 cctgttagga ctcctcttca gcgaactttt tgacgtttct ctccaaatga aaaaactcta
      781 ttttccaggg aaaatagagt tagagttatt tgatcaagcg tcgtcaaata agctctatct
      841 tctttatcca gactatactg tcggtatcgg aatttcaccg attcagctat ttttttgcaa
      901 aataggtcgt ggactataac caccggtcgg gaatttcacc ctgcccctga agacgaacct
      961 tatataattt ttgtttacat attcattata catatctatt caaaaaacgc aacatctaac
     1021 acttataaaa agtaagtgtt cattttgagt ggttcagcaa actgctgact tcttttttgt
     1081 tcagttcacg gtaatcccca ggacgtaggc cttgtaaagt cagatctccg tatttttcac
     1141 gttttagttt ttgcacagga agaccaactg cttgaagcat atttttcact tgatgattac
     1201 gtccttcgtg gatcgtcaat tccacgatac tgtggtttgt cttaggatcc actgataaaa
     1261 tttggaattt agctggagat gtttttcgtc cttcgatctt tattcctttt gtcaaaggtg
     1321 caagcatcga ttttgtagcg atccctttta cctttgcaac gtaaacttta tccacttcat
     1381 gttttggatg tgtcaggcgc tgagcaaaat ctccatcatt tgtcagtagc aagatccccg
     1441 aagtatcgta atccagtcga cctacaggat agatacgttc ttttaccatc ggcaaaagat
     1501 ccgtaacaac ttttcttcct ttgtcatctg caacagcaga aataactcct ttaggtttgt
     1561 acaataaata gtaaccgtat tcttcttgat aaattgggac ttcatccact tccacagtgt
     1621 catgtttccc aacttttatc cctaattccc ttacaacttg tccgtttact tttacacgtc
     1681 ctgcaaggat gaattcttct gcttttcgac gagaagtgat gccagcgtgg gcaattactt
     1741 tctgcaatct ctccattaca attattctcc tttattcatt ccatcaaaga atagatctgg
     1801 tgacacgtct tcttcaagtg aaatttccat ttcttgtgta tcaggcagtt cttctaatga
     1861 tttcaaacca aaataatcta agaagtagcc agttgttcca tataatatcg gccgacctgg
     1921 tccttctact ctaccttttt cttcaatcag tttatgggca gctagacgct gtaccgctcc
     1981 tgaaacctga actcctcgaa tctgctcgat ttcagctctt gtgattggct gtttataagc
     2041 gatgattgct aaggtttcga cggcggcttg agataatgtg tttgatgtcc cttgcgcata
     2101 tcgcttaagt aagggagcca gctctttttt agtcgttaat acataatggc tttccgtttc
     2161 aaaaatatgc aaagctgagt gcgagttttg agtgtaagat tcttttaatt ctttcactaa
     2221 acttgtgact tctgcttcgc tgagatttaa cagtccgctt aattcttcat tactgatccc
     2281 ttcctcgcca acaataaaaa gaagggcttc aagttcactt aatttcgtca tgttcggtat
     2341 ctcctactgc tggataaagc ataattgatt catagttttc tttttgttgc gcaatgatct
     2401 gccctttttt catcaattct agcaaagcca taaaagtagt gacaacttca cttttagaag
     2461 gtgactcaaa aaaagaatcg aaagtacagc catgcgcatt tctttttata cgctcttcga
     2521 taaataccat tcgttcttcg atcgttgtgt catcgggcgt gatcgttttt tccacttttt
     2581 gagaaaattt cttacgttct aagattttct gcatggcacg gatcaaatcg atttttttga
     2641 cttgtcctcg cttcaacggc ttttcagcat ccgctaaccc atcgatgttc ataggttctt
     2701 ttgtgtaata aagacttcgt tcctctgctt tttctgtcag tctttcagct gcatatttat
     2761 atttacggta ttctaaaagc tgagtgacca attcttctcg aggatcttct tcccataatt
     2821 cttcatcgtc tgtgacgaat tcttggacag gaagcaatgt cttgcttttg attgccatca
     2881 atgtagcagc catcactaag tattctcctg caacagctag ctcgagtgtt ttcattgtat
     2941 ggatataatt catatattgc tccgtgatcg tagcgatcgg tatatcatat atatcgattt
     3001 ccatttgctg aatcagatga agcagcagat cgagaggacc ctcgaataca tctaatttaa
     3061 tattgatctc cgtcaactca ttcactcttt cttctttttc tttgctagcc attttccaat
     3121 gtaaggctct gtgatttcag ttgcacggat ggagacatcc gggtactttt cctgtaattc
     3181 atctaacatt tgaaagccca tattctcacc gcgataagac ggattcaaag aaatgtcatg
     3241 gatgatcagc tgatcgttcg ttgcccagta aataccaata atcccttgaa tgttggttga
     3301 accttctggc gtaaagaaaa aaagttcata atcgtcttca gattcgtacg tatcaatctc
     3361 cttcaacaaa gaagttttac ctgtcagttt cttatggaaa ctcaatagtc ccatagcaat
     3421 tttacggttt tctttccggt aatgtgttaa catcttctca ccttcctatg actaagccct
     3481 tggaaaatgc tgcttataaa catccgtcat tcttttcttc gtgatatgtg tataaatctg
     3541 agtcgtagag atatccgcgt gacctaacaa ttcctgtact gtccgcaaat cagctccatt
     3601 ttctagcaga tgtgtggcaa agctatgccg cagcgtatgt ggtgttactt ctttgtaaat
     3661 cccagcctct cgtactaact gcttcagatt tttccagatt ccttgccgag aaagaccttt
     3721 cccatgatta ttgacaaata catgcatttc agatgcgtcc tttacaagta atggacgtgc
     3781 ttcgtctaag tagcgttcca gccattggat cgcataatca cctaaaggaa tgatccgctc
     3841 tttatctcct tttccaactg tctgtaccag tccaagagaa agatgaagat ctcccaattt
     3901 caatccaatc aattcactga cgcgcatccc agtagcatac atgacttcca agattgcacg
     3961 atctcttatc cctaacgttt ttgtcgtatc aggtgcttca atcagctttt ctacttctgt
     4021 cagagtcaat gtacttggta atttctgtgc tttcttcggc gtatcgatat gttgcatcgg
     4081 atcatgatca gtgatacgct cttgccgtaa aaactgatgg aatcttctga gacttgagat
     4141 catccgagta acagttgctg tcgctttttt ttcttcatgc aacttctcca gaaaacggat
     4201 gactaaaaag cgatcgacat cctgccatga agcaattttc tgctcctgta gaaaatgcaa
     4261 gtactgttcc aaatcacgtt catagctttg tcgtgtattg gcggacaatc cccgttcaat
     4321 attcaagtag tggagataat cgttgatttg ctctttcata ataatccccc tcatgcctct
     4381 ctatcatatc agataccgct tcattctgat agtctttttc cataatctga gcaaagaaaa
     4441 agccgtctag acactcagac ggactggttt cttttttctt tttcatagca ttcctgacag
     4501 attccatgga acgttagccg atgatctttt actaaaaaat gataacgttc ttctactact
     4561 tgctccactt caagtaaaag atcctcttct acttcctcga tcgtgccgca gttcaagcat
     4621 agtaaatgat gatggaaatg tttggcgcct tcttttctca aatcataacg ggctaatcca
     4681 tcattaaaac ttactttatc gacaattttc aattctgtca agatttcaag ggttcgataa
     4741 acagtagcaa gtccaatttc cggactcttt tttttcacta aaaagaagat ttcttctgca
     4801 gataaatgct ctttctcatt ttccaaaagc acaagcagag tagcttctct ttgcggtgtc
     4861 agcttaaacc ccgcttcatg tagctgctgt tttgtttttt tcatcgccga aatagcattc
     4921 atgaccattt actcaacccc aacaatttat aataattaca aataaaaaaa gatataacac
     4981 tatgttaatt aaaattatta taagctagtt aagaaaaaca tgcaagggat tctcaattag
     5041 attttttgac tagttttgtc acaccgtttt cttgtgtaat caatccttgt ttcaaaagat
     5101 tacctaatgc gcgcttaaat gctcctttgc tgataccaaa agctttcatg atttcttctg
     5161 gatccgattt atctgtaaag ttgatttgct gatctgcaga acgttctaaa atggttaaaa
     5221 tcatttgtgc atcatctgat atagcttcat atgctcttgg cttcaatgaa agattcagta
     5281 caccgtctgg tcggacacca atcactcgac cttttatctg ctcgcctaga cgaggttcat
     5341 tatatcgttc agaaggatgg ataaacccaa gatagaaatc ttccgttaaa acgtacgtac
     5401 ctgttaattt caagcggtat gctgttccta tcacatcttt gtttttcatt tcttctgttc
     5461 catatttgct taaagatttg aatatttttt catctgctaa actgccccaa atacggtctt
     5521 ttgcatcaac tctaagggaa atcatcaagc gatccccttt tttaggccat agctctgtca
     5581 ttgtcggcaa ttcatcaaga gatacgacaa agtctttatc tggaagaccg atatcgacaa
     5641 acgcacctaa atcttttcgt gtagcagtaa cgattccaaa agcgtaatga ccgatacggc
     5701 ttttaggaat ttgtgtagtg atccgatttt catgtttttg attttggtaa ccgaatcctt
     5761 caactgcttc accgattttg tgttcacctt ctgatttgct taagccaaag gtttgtccgt
     5821 ttttttggac aaagtagttt ttttcatttt catcaataat aatacctgta aatatagttg
     5881 ctaataaatc gttcattttt ttctccctct atagtgaaag gttttcttaa tacatttctt
     5941 tgctatcata acataatgtt atctacttga atagctgagc cttggtttgg aaaggtgaaa
     6001 aaaggagtat gacaacagtt gtgtcatact cccctttttc gaataaacgg tgttaaacgg
     6061 caggctcaca gcctctttca tttgcctaaa attaattgct cccatttcct ccgaaccatc
     6121 cagttttaca gttgatggtt acgatgtaaa atggcaaagc actttctcca atgcctttca
     6181 tcaatgcaga ttttgttgaa gtggcatata gattataata gccttcccca ttttcgattc
     6241 tggctttttc taatctgtaa cctccttcaa cgatatatcc acgttggata catgtagcaa
     6301 tcactttgct ttggacacct ggtgcccacg tccatgctgg attagatgca tcggcaatat
     6361 cacttgctct ggcagatttg attggctgat tcgctgagtt tgaagaggaa ggggtcgagg
     6421 atgccgcttc ttgctgtgct gcttgtgcga ccgcagcttg ttgagcggct tgagctgcag
     6481 ctgctgcttg agcggcttgt tttgcttgtt cttcagctgc ttttttcgct tcttctgctt
     6541 ttttcttttc ctcttcttct tttttctctt tatcagtgag catcttatct acttgtttca
     6601 attgttcatt caactgagca gatagatctg tatttttcac tttttctact gcttcttttg
     6661 ccgtcttgta ttggtcgcgt gttgctgaat ccactacttt tccgtcttta aagactatag
     6721 ctgttttttc tttagctgtt tgcaattcag catattgatt tttagcttct gtttgtgctt
     6781 gttgaatcaa ttttccaaaa tctgtgttat ccgtttcgat tgcttggatc tctttatctg
     6841 ctttcaactt gatgtcatct gctagatgat catcgacgat ggcaggtgct atgaaccact
     6901 cattcacttt ttggatatct cggatcttag attggatatc ttggtaagtt gtttctaact
     6961 ctgaatatcc tttttcattc ttaagactgg ataaatcatt ttttagtttt gataaatctt
     7021 gatgaatcat atctgcacga ataaattgat ggtgatcatc tgtataaaag gcagcaagtt
     7081 tctcatgaag ttcttccatt tgttgctgtc tttttgcttc tgctgctact tcggcttcta
     7141 ctttttgatg atggtcataa taagcccagc ttcctccacc gagtagaagt actgctgcta
     7201 aaacgagata aggtactttt tttgattttt tcttttgcgc tttatctgga tctgtttttt
     7261 ctaagtttga ttcttgatgt gttttttcag tatctggcac gattgttatt gatttcgctt
     7321 ctgcttcaaa cagcttaggc tggttctctt ctttttctat ttttgcttca agatcagtac
     7381 cctctttgct tacagattga ttttcagcat cggattcact atttatttca tcgggctctg
     7441 ttgacttttt gtctgtgttt tcgagtattt cttttttctc tgcttccggc agtttattcg
     7501 ttttttcctt ttctaattgt ttttcagtaa atacttcagt tttttctgtg atatccgttt
     7561 ttacgtcttt tatttttact gtttctttac ctgtggcgtt ttctctatca gagtcttttt
     7621 cagtttcttg ttccttttct ggctctactt tgttttcctg tgcaattttt tcactgatca
     7681 ttttgagcag ctctttcgcg tgctcttctt ttgtatcttc acgatgtgct ttgatataag
     7741 cggatagtat cgaattttct tcctcattat cgacgatttc tttcagttca gctactcttc
     7801 ttttttgttt tgattcaagc gtttcttctt gattctttcc tgattcttcc gaagtctcat
     7861 gaacagttgc tttcttctgt tcatttttct ctatattagt tacttgatcg tgagcaggtg
     7921 gttcttcgtc ttctggttct gctttttcga ataattccat tacagattct agaggtaaat
     7981 ctttcaattc cgaccattcg attggatcgt ttaattctgt atcaggaaag actgcatctt
     8041 cagttgattt atcctgcata gctgcatctg tatttgtttc ttcaattgat tcttttattt
     8101 gctgatcttc tgcagttatt tgatgaccgc agtctggaca gatagtattc tgctccattt
     8161 ttttcgatcc gcaatttgga cattttttca tcacttagcg atcggctcct ttactaggtt
     8221 tacgctacca tttaatctat catattctat acgtcacgcc aatagcttat ttttaggatt
     8281 tacttaaaaa taatgaatga atttccttcc tatagaaaaa aaagcttgag actgcgtagt
     8341 ctcaagcttt ttttctttta aaataaacca aacagtttta ctgatttctc ttctggtcaa
     8401 tagtccacgc ttattctaag aaatctttta attgttttga acgagaagga tggcgcaatt
     8461 ttcttaatgc tttagcctcg atctgacgaa tacgttcccg agtaacacca aatactttcc
     8521 ctacttcttc caatgttctc gtccggccat catctaaacc aaaacgtaag cgaaggacat
     8581 tctcttcccg atcagtcaat gtatctagta catcttccaa ttgttctttt agtaactcat
     8641 aagctgcgtg ttctgctgga cttgttgctt cctggtcttc tatgaaatca cctaagtgag
     8701 agtcatcttc ttcaccgatt ggcgtttcaa gggaaacagg ttcttgcgcg attttcaaga
     8761 tttctcggac tttttctgtt ggaagatcca tttccgcacc gatttcttct ggcgtaggtt
     8821 ctcttccaag gtcttgcagc aattgacgtt ggatacgtat caatttatta attgtttcaa
     8881 ccatatgaac tggaatacgg atcgtacgtg cttggtcagc gattgctcga gtgatggctt
     8941 gacgaatcca ccatgtagca taggttgaaa acttaaatcc tttgcggtag tcaaattttt
     9001 caactgcctt catcagtccc atgtttcctt cttggatcaa gtcaaggaac tgcatgcctc
     9061 gtcctacata acgtttagcg atacttacta ctaaacgaag gtttgcttct gccagacgct
     9121 gtttcgcttc ttggtcgcct tcttcgattt tcaatgctaa cgctacttct tcttccgcag
     9181 ttaaaaggga gacacggcca atttctttca gatacatacg aaccgggtca ttgattttta
     9241 caccggttgg cgcagaaaga tcttctgttt ttgcttgttc agcttttttc tcagcactct
     9301 tcaagctgtg aattgatgga tcaccatttt catccaccac actgatacct gcgtcttcaa
     9361 tcttttggat caatttgtcc atttcatctg cattcaaagt gaatggtgta gcaagctgat
     9421 tggataaatc atcatataca acttgtcgtt tgggtttgtt ttctttgatg aaagctgcta
     9481 ctgctgcttc atacgttttt ttatctgttc catttgccat aaaagaaggc cccccttgag
     9541 tagctatttg gcttgtttta actgtttcgt taaattgatg atttcaatcg tcaattcaag
     9601 ctcaagctgt ttgtttccag ttcgtctggc ttcttgctgc atttgttttt ttaatgttat
     9661 ttcatcagct aagccagatt tgctgatcac atctagcaag tcttggattt ctcgtggcga
     9721 actttcttct gccaccggta aatattcgat ttccaccacc aaccgcctta aattttcatc
     9781 ttgcaaaaag tctaaaaact ctgcaaggtt gaattcataa tgttgtgaga catatgcatc
     9841 gaataaaaaa taaacttctg catatgcatc atgaataaac tgaaattctc gttcttttaa
     9901 aatattacgg acagaaactt ctttaaatgc acgatataat agaatctgct ctgccttttc
     9961 tacttgcgtc aaaggtctct ttttccgtgc agtttcttgt ctcgtaacct gagtgtgttg
    10021 actttcctga ggttcttgac gattttgctg atttgtcctt cgaagatcgc gcagttgttt
    10081 ttgtatcgtc tctctgccga tctgaaactc tgttgacagt tgagatagat acatatcttg
    10141 ttcgattggt gaaacaactc ggctaagttc aactagtaat tcttcgaggt attcgatctt
    10201 gtctttttca ttatctaaat ttttcccttg ttttaaataa cgtgatttaa aagtaaagac
    10261 tgtttcgcgc ccatgagcca ctaattcttg gaaagatgct tctccatact tacggagata
    10321 ttcatcagga tcaagctttt ctggaacgac tacgacagaa agtgctaatc gactgttttg
    10381 ccgaagcaat tcaatccctc gattggttgc ttcaaaacct gcgttgtctc cgtcatagca
    10441 aaagaccaat tctttcgtca gcttttccat ttgttggatc tgttgagctg ttagacttgt
    10501 ccccatagaa gctaatccag ttttgatacc agcctgccat gcagcaatca catccataaa
    10561 cccttcgaac aacaaaattt ctccgttttt ccggatatcg cttcttgctt tatcaagatt
    10621 aaataaaact tgtcttttat tgaacagttc tgtttctgga ctattcaagt atttgggctg
    10681 atctttttcc tgcccttttt caggtgcaag ccagcgtcct gaaaaaccga tcgtctggcc
    10741 ttgagaatta cgaagtggaa acatcactct ttgataaaaa cgatcagaca tacggccatc
    10801 atcatgcgtg acaaacaatc cagagcgttc taacaattca tttgaaattg attcattctt
    10861 gaatacttgc tgaagaaaag aacgctcatt aggtgcaaaa cctatgttga attcttcaat
    10921 taattctaaa gacaacccac gactttgcaa atattctaaa gctggctgtc cagcttttgt
    10981 atgcaatagc atgtgatgat agatttccgc tgctttttca tgagcagcaa tcagtttccc
    11041 cgtagcagat tcaggatttc ggacagctat agtacgctga cgccattgat cttctaacgg
    11101 tatctgttcg aagtctgcca cttttaccac tgcttctgga aaacttagac cttctagttc
    11161 ttgaagaaaa gtaaacacgt ttccgccttt accacaacca aagcaatgaa aaatctgctt
    11221 gtcttctgca acagaaaaag acggtgtacg ttcttcatga aaaggacaca gccccaagta
    11281 atttttacca gactttttca actggacata ttgtccaatc acctcaacga tattcgtttg
    11341 actgcgtact gtttcgataa cctcttgagg aatccgttga gccatttttt caccccctgc
    11401 tctattgata tcttttgggt ttaacagaag atatttcagg agaataaccg gtaaaaaatc
    11461 gcactaactg aaatcagtgc gattttaaag gacacaatta tacatgtttg attgtatcat
    11521 atttcattgc tttttcaagt ataacacatt tctatctcat taagcatcaa tttgctactc
    11581 ttttcagtaa ctgattttga atatcagcct tccagtacaa tactcgaaga acaatattta
    11641 cgagcatgaa tacaaagaag tatcctacgc tctctcccaa ccctaagttt acttccaaaa
    11701 ttccatggaa cagcattgca gctacatata tccctacgat cgtcgttact gctaggaaga
    11761 tgcgcaatgg gttgaaggga taacaagctt ttacaactgc catgcaactg atcccaatca
    11821 gcaagtaata catcaaagta gtagtttcag catttcccca tgcgaatgtt tttccaagta
    11881 gatacaccgc aatgatattc actactacaa gcaaggcatt tggtaaagca ttaataagtg
    11941 ctgtaggcaa ataacggtaa gtgatttttc ttttatcttg ttcaaaagat aaaaagaagg
    12001 aaggataccc ttcaatcgct aaatcaatca gcgtgatttg gatagggata aacggaaatc
    12061 caattgctgt aacggcacat ataatcgata aaataaatga ataaatcgtt ttgataaaaa
    12121 agatgcctga gactttcgtc acattgttga ccactcggcg tccttcaaac aggacttctg
    12181 gcaatgtagt aaaatcagag tccaacaata ccagattcgc aatctgacgt gttgctccat
    12241 caccttctgc catagcaatg ctgcaatctg cttctcttaa cgccaaaaca tcattcacac
    12301 cgtcgcctgt cattgcaacc gttctgccgc tttctttcaa ttcatttacc agcaatttct
    12361 tttgctgcgg actcactcgt ccaaaaaccg tatagcgatg agcagcttct cgcacttcca
    12421 tttcttcagt aatttgagaa agatcgacat acgcatcata attcggtaat cctgcacgtc
    12481 tagcgatatt ggagacagta aaaggattgt ctccagaaat cactttcaga tccacacctt
    12541 cctcatgaag ataagctagt gtttcttgag cgttttttcg gattggatca tcgatttcaa
    12601 acagagcaac cggctcaaca aatgctggtt tttcttcgtt caattgctga tttggagcga
    12661 tccccaacat aagtacgcga tagccattcg cttgggctgt ttttagactg tccggaagcc
    12721 gatcttcttc caccaaacgt tcaggcgctc ctacgtacac gactcccagt tctggaaatt
    12781 ccatcgcgcc ccatttgcga tcagaagaaa atgcgacttt gtttgtcaca tcataccgat
    12841 tagaaagctc aaaataatct ctcatagccc gcatagtgat gttgttatca ctgctttctg
    12901 ccagatacgt ccccataatt tctgggatgg ctttctcgta cagcggatgt agtgcttcta
    12961 cccgctggat actcattttc ccttcagtaa tggtccctgt tttatccaag cataatgtat
    13021 cgacatgggc cagcgtttca attgaataca tgtcttgaac aaggattcgt ttttttgcca
    13081 gtttggttac ccctgtcgtt aatgcaatac ttattagcaa taccaaaccc tttggaagca
    13141 tgcctaacaa agctgctgca gatgcaataa cagaaatttt gatagaagca tcgcggatga
    13201 acagtgcttc caaaaatagt atgatcccca aaggaataat cacgaaacta gtgaatttgg
    13261 atactttgcg aatcgaatct acaagttcag aacgaatagc tttgtgagtc tttgcctcgc
    13321 tagtcaagcg gacagcataa ttatctgcac ctactcggat aacttcagcc atgacagaac
    13381 cgcttgtcaa aaagcttcca gaaagcaatt cagctccagt ttctttgaca atcaaatcag
    13441 attcgcctgt caaaagagct tcatttgctt ctgcccgtcc agatataacc tgtaaatcgg
    13501 ctggtatctg ttctcctgct ccgattttta ccaaatctcc taataccagc tctttagtag
    13561 aaatcgattg ttctttaccg tcccggatca ctagtacttc tgattgggaa acaatcgata
    13621 atttccgtac aagatttctt gcatgtattt cttgataaat tccaataacg atattgacga
    13681 caataattgc taaatacgcc atgttggaat acgcacctac aaataacaga caaatcccaa
    13741 taccaaagtt cagaaaattg aataacgtca atatattatc gctgaatatt tgttttgtac
    13801 tttttgatgc atcttcctgg tagtcatttt gctggccaga agccattttc tgaactacct
    13861 cttgctgtgt aagtccattc attttctttt tctccatttt ccgccaactt tcaaaaataa
    13921 tcacgtgttt aagtctagcg tgttccaaga ttaaaaacaa cttgttttta taaaatttat
    13981 agaaaaacac acgaatgatt ttacaaaaaa gatgccaacc tttttgtttg aatcacccgt
    14041 gtgctgtttt tattatcaat ctatctttac ttatattgtt cctccaaacg gctcatccac
    14101 caacctagtg ctgtaccggc tataaaaaga acaggacaag ctagtacccc agcaactccc
    14161 aatcccgcgt aatcggtagg aatgatcata atcgttgaag caaaaactac acctaaaatg
    14221 aaatggaata gcttgctata tgcctttgag aaaatgtaat ccatgatttt tgacaatcca
    14281 agaacacaaa gaatcccacc aattccgatc ggcacaatca cttcaagtcg tacttcttta
    14341 aatccgtcag ccatcgcttt atacaatccc atatacacca aaaaattaga agggctcaat
    14401 cctgggacga tcatacccaa accaatcaat ccgccggcaa tcagccatgt tccaaaattc
    14461 tgatcgactt gcccgtcaaa taattgagcg ccgtataata aaaacaaata agccagaaca
    14521 aaagtaacaa ccatgatgat cacatcagaa gtatctcttc cttttttccc tgcttctttc
    14581 cataacgcag gaacagttcc agcgatacat ccgacaaaaa accataatat cgttgtttca
    14641 tacgcaccta ataaaaaact gactaaaaaa gaaagtagaa agatgcctgt cactccgcct
    14701 agaccaacag gcagaaaaaa caaaacattt tctttgaaat tttttgtaag gtgggctaaa
    14761 aaagaaatca gccgctcata catgccaaag acagctgcta atgcaccacc gctgactcct
    14821 ggcaagataa atcctgaacc gataaacatt cccttcacaa accgtaacat ccagtctttg
    14881 gttgtgtacc ccacttcttg attttccatt ttttgccttt tttccactat ttgcgtactc
    14941 cttctttcat ttaattttat tcatgattga acatttcctg ctattttact taaagacttt
    15001 ttcataggtc tctattattt tttcattcat tttgacaata tcaggataaa acatgttacc
    15061 tccattatga tagagatagc cgccattgta aagcaaggac tggatccgcc aatatcggta
    15121 ttgtttccct tcctcatttc ctaaaagtgg gctcagcaca tctttagaat acgcttctgc
    15181 taattctact gtgttttctc cgccatgatt ttttacatag gcaatataat ctttcccaaa
    15241 attatatgct tggacagctg tccataaatc gcatccttgt ttctttgctt ctactatcat
    15301 ttccgccaaa tatttgacac cttgatcaat actgtcagct ggattatcaa tgatctcagc
    15361 ttctccatgc agactttccg agctctgcat gagatctgtc ccttttcctt ttgattcggt
    15421 caatattatc gccttgatca aattctcgta gttttccata tgataatttt ttgcagctgt
    15481 tgttatttct atttgatagc tttccactcg ttttaccgaa cgataagttc ttcctcataa
    15541 aaacaacacc gcaccaataa ccgtcagaat gatcatgtaa aaaagaatac ggataataga
    15601 taatcgtttt cgcttcattt catcacctag ttattttgac atcttttttt atcttactat
    15661 ttctttatct tcttggctat ttcttccttg atttctgtgt acgccttaag aaaaaatgaa
    15721 ttttcctgta ttttttgaca tcttacgaaa atgatcgttg tttttcagta aaaacaattg
    15781 cccaatgact gatataatcg ttatacttta cacagtaggt gattatgtga aaaaagaaaa
    15841 gaaatatgca aaaatgccag atgggagcga aatttactat gaaaagagcg gtcaaggctt
    15901 ccctctcttt ttactgcatg gcaatgacgg cagcggccgc tttttttcag aacaggtccc
    15961 tgtactcgaa cgctactata ccgtgtatct agtagatagc cgaggacatg gtcgttcgac
    16021 aaacgaagca tctatgttga attttcagtt aatggccgag gatctgaata cgatcatgtt
    16081 gctggaaaaa atcgatcagg cagattttct tggatttagt gatggagcaa atctagcact
    16141 ggtatttgcc agcagcttcc ctaaaaaagt tcatcgattg attttaaatt ctggaaacac
    16201 gcttgttaaa ggcgttcgtt tttctgccag agtcatcagt aatattcatt atgcatgggt
    16261 atggttactc tctttatttc gcccaagtct tcgcaaaaat cttttagtta tcaaactttt
    16321 gctgcatgac atcgggctta cagaaaatga tttaaaaaaa atcaactccc ccactttgat
    16381 catcgtggga aaaaaagatg taatcaagct aaaacattct ttgtatatag ctaaaactat
    16441 accaaaagct tcctttgtgc ttgtgaaaga acaaggacat gaattagcta gaaaagatcc
    16501 tgaacgcttc aatcgagaag ttcttcaatt tctttctgaa acataactat taggaataga
    16561 aggtgattta tttgttaaaa aataccatac aatggctgaa aaaccaccta ggactgttca
    16621 aaacaatctt tttgatttct gtcattgtca ttattgtcgg tgaattgatg tccatcggta
    16681 aaactctatc tatccagcaa ttaggagaaa cgtttgccac tatacctgta tggaaaagcg
    16741 gactaatgct attgattggt cttatctctg tgttacctat ggttggttat gatattattt
    16801 tgaatcggat gcttgaacaa aagcagaatc ctcgttacct gtttgagaca agctggctga
    16861 tcaatacgat caacaatatt gccggtttcg gcgggtttgt tagtgtgggg cttcgctctg
    16921 aactatatgg acaaaagaaa gagagcagaa acgtgatcca agcattatcg aaagttttct
    16981 tgtttttgat ggctggactt tctatttaca gtctgctttc ttttttactt gtagtcttca
    17041 cgccagttgc tccttttttg aagcaatatt ggatatggtt gatcggcggc ggtctatatt
    17101 ttcctgtcgt ctttttattt actactttca aaaagaaagg attccttggt ggtacctcac
    17161 ttaaaactca aggacagcta ttgctagttt cattgttgga atggaccggt gtcctgttta
    17221 gtttcctttc tgttggttat ttaatggaaa tccggattga tctgtggcaa acaattccat
    17281 tattcattgc tgcttcagtg ataggaatcg tgtctatgat tccaggagaa attgggagtt
    17341 tcgatgtaat gatgattatc gggttgtccg ctattggtgt gccaagagaa atagtcgttg
    17401 tgtggatctt gctctatcgc ctgttctact acatcattcc gtttttgatt ggaatcgtct
    17461 ttttcttcaa aaatatcggt tccacttttg atcagcgtta ttctggtatc cctaaacaat
    17521 tagcaacaga aattgcgcat aaaatcgtgg ttgtcttatt atacttttct ggaatcatgc
    17581 tagtactttc tgccactatc ccgcaagctt ttacagagtt tcgctggctt catagtttga
    17641 acccattgaa atttcatttt attatccaat ttcctagtat actattggga tttcttttaa
    17701 tcattatggg cagagggatc gcagcacgtg tgaaacgagc atatttgcct acaatcttct
    17761 tgattgttct agctttactt tatgttttat taagtgattt tagtttcaca ccggtggttt
    17821 tcttgtctat tctgcttctg atcattcttg cttctaaaaa cgaattattt cgggaacaat
    17881 tgatctattc atgggagtgg cgtacgatcg atggaatcat tattggtgca ctgtccttgc
    17941 tttacatcgt tattggggta tacaatctgc cggatttccc tcatcggcgt caccatttta
    18001 tttccttttt cttatttcct tctgaaaaga tttggttttc gggattgttg gccatcatcg
    18061 ctgttagttt catgatcgtt ctatttgttc atttccttca aggagaaaag aaacagattg
    18121 gcgaagcctt taatgaagaa aaggccctga agatccttac aacttatgga ggaaactccg
    18181 atagtcaatt gatcttctta aaagataagc ggatgtttgc ctatgaaaaa gacggtgaac
    18241 caactgttct tctccaattc gcttgcttca ataataaatg tattgtaatg ggtgatcctt
    18301 ctggtaagaa agaagatttt ccagaagcaa tcgaagcttt tatcgaagaa acagatcgac
    18361 tatgctattt accagttttc tacgaaacaa gcgaagagat tgttatgatt ttgcatgaat
    18421 tcggctacga tttcattaag atgggggaag aagcttatgt agacctgaat agttttacaa
    18481 cttcagggaa aaaaatgaag ggcactcgtg ctgtcttgaa tcggatcgaa cgagaaggat
    18541 ttacatttga tgtcttacaa ccaccatttt ctactgagca aatgagtact ttcaaaaata
    18601 tctctgataa ttggttgggt tcacgaaagg aaaaagggtt ttctttaggc tttttctctg
    18661 aagattatct acaaagagcg cctattgcag ttgtcaaaaa tacccaagaa gaaattgtcg
    18721 cttttgcaac gatcatgcct acttacacga acaataaagt aggaacgatt gatctgatgc
    18781 gatatgattc taaaaaagca ccttctggca gtatggattt cttgttcttg catttgtttg
    18841 cctatatgaa agaagaacat atccaatggt tcaatttagg gatggcccct ctttcaaatg
    18901 tcggaacttc cagaaagagt tttttacagg aaaggattgc atacttggtt tacgagtttg
    18961 gttctcattt ttattctttc catggattaa gagaatataa aagcaagtac gctacaaact
    19021 gggtctcacg ttatactcta tattctagag atagttggat tgcatatgtt atgattgctt
    19081 tgctgattat tgataatgcg ccagttgagc agacacaagg tagaataagt ggtttgaaaa
    19141 aatggatacg aagaaagtat taaaaactgc ataccgatag atgaacactc tcatgatgaa
    19201 taaaagagac tgaagtaaaa cgaaagttca tgcttctaca atctcttctt atacaattga
    19261 agaacaaaga gcggtccaaa cagaaatcct gtttggaccg ctcttttatt ttaaacattt
    19321 ttcattacca aaatattcga gtcgcgtttc ttatgcttcg atctctgtaa cgatacctga
    19381 acctactgtc cgtccgcctt cacgaattga gaatgtcgtt ccgttttcaa cagcgattgg
    19441 atggatcaag tcaacatcaa tcgtcacgtt gtcaccaggc atgaccattt ctgtatcttc
    19501 tggcaatgtg attgttcctg taacatcagt tgtgcggaag tagaactgtg gacgatagtt
    19561 gttgaagaat ggagtatgac gtccaccttc ttcttttgtc aacacgtaca cttctgcttt
    19621 gaattttgta tgtggagtaa tggaacctgg tttagcaagt acttgtccac gttcgatatc
    19681 gtcacgttgg atcccccgta acaacacgcc tacgttatcc ccagcttccc cgtaatctaa
    19741 cgttttacgg aacatttcta cacctgttac aactgctttt tgtgtttcag gtttgatccc
    19801 tacgatctct acctcatcac cgacacgaac agcaccacga tcaatacgac ctgaggcaac
    19861 agttcctcga cctgtaattg agaagacgtc ttccactggc aatagtaatg gtttatctgt
    19921 atcacgttct ggtgttggga tatattcatc tactgtatcc atcaattcca taatagcagc
    19981 ttcagcatct ggatcgcctt gcaatgcttt taatgcggaa cctttgatca caggagtgtc
    20041 atcgcctggg aaaccatatt cgctcaataa ctcgcgaact tccatttcta ccaaatcgat
    20101 caattcttca tcatcgacta aatcaacttt gttcaagaaa acaatcaggt attttacacc
    20161 aacttggcgt gacaataaaa tgtgttcacg tgtttgaggc atcggaccgt cagttgcaga
    20221 aacaactaag attgcaccat ccatttgtgc tgcccctgta atcatgtttt tcacatagtc
    20281 cgcgtgtcct ggtgcatcaa tatgcgcgta gtggcgtttt tctgtttcat attctacgtg
    20341 tgcggtattg atcgtaatcc cacgttcacg ttcttctgga gctgcatcaa tactagcata
    20401 gtcttgagga tttgctaggc cttttttgcc taatactgta gtgatcgcag cagttaaagt
    20461 agttttccca tggtcaacgt gtccaatcgt accaatgtta acgtgtggtt tacttctgtc
    20521 ataatgttct tttgccataa tttacaaacc tcctaaataa tatagatcac tgaaaaattt
    20581 cagctctcac ctttttatta tagtcttttt ttgactaaat caaaaagaat acgtccgact
    20641 tactttttgt aagattcgaa ctaagcttat tatacgtcaa tattggtcaa agtcaactaa
    20701 gagcactcgt ctgtcttata cgcgaaaaaa gtacctttta taaataaaaa aatgtgtttt
    20761 ttgagaaaaa cttcaagata gtccttaact ttcttttgaa gcaataagaa caaaatgagg
    20821 aaaagtttaa aattcagctg ttgccaacaa ctttcaatag acgaaatctt ctatttcgtt
    20881 aataataagg aaagaggtga agtacatgaa aaaattaatt ggcgtacttg tcgttttagg
    20941 catatttctc ggcggaagtt ttgcagctta tcattatttt tacggcggcg aagcttatta
    21001 tacaaaaatc acgacgaatg gcgaaatgtc agctacccaa gcagataacg gagagaaatt
    21061 cacgacttat acctacaagc aagcggctta taataaagat ggtgaaaaaa aacaggtgac
    21121 gcttcgcgaa gaaagagaac atccattaaa gatgaatgcg tatcttaaac tcaaagtcaa
    21181 tcctcgtaaa ggtgtattaa gctgggaaga aataaaggct tctgaagtgc ctaaaaatgc
    21241 ggcagaaaaa atcgataatt agtaaatggt cttaaaattt tattagataa aa
//
LOCUS       DAZKOK010000044        20845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000044 DAZKOK010000000
VERSION     DAZKOK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.044"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..629)
                     /gene="pssE"
                     /locus_tag="ACG90W_001389"
     CDS             complement(150..629)
                     /gene="pssE"
                     /locus_tag="ACG90W_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0477762.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(646..1107)
                     /gene="pssD"
                     /locus_tag="ACG90W_001390"
     CDS             complement(646..1107)
                     /gene="pssD"
                     /locus_tag="ACG90W_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0477763.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(1121..2371)
                     /locus_tag="ACG90W_001391"
     CDS             complement(1121..2371)
                     /locus_tag="ACG90W_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0477764.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(2749..4200)
                     /locus_tag="ACG90W_001392"
     CDS             complement(2749..4200)
                     /locus_tag="ACG90W_001392"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0477765.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(4521..5285)
                     /locus_tag="ACG90W_001393"
     CDS             complement(4521..5285)
                     /locus_tag="ACG90W_001393"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0477766.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5313..6011)
                     /locus_tag="ACG90W_001394"
     CDS             complement(5313..6011)
                     /locus_tag="ACG90W_001394"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0477767.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6023..6802)
                     /locus_tag="ACG90W_001395"
     CDS             complement(6023..6802)
                     /locus_tag="ACG90W_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0477768.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6818..7762)
                     /locus_tag="ACG90W_001396"
     CDS             complement(6818..7762)
                     /locus_tag="ACG90W_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0477769.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7808..8587)
                     /locus_tag="ACG90W_001397"
     CDS             complement(7808..8587)
                     /locus_tag="ACG90W_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477770.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(8967..11042)
                     /gene="glyS"
                     /locus_tag="ACG90W_001398"
     CDS             complement(8967..11042)
                     /gene="glyS"
                     /locus_tag="ACG90W_001398"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0477771.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(11044..11961)
                     /gene="glyQ"
                     /locus_tag="ACG90W_001399"
     CDS             complement(11044..11961)
                     /gene="glyQ"
                     /locus_tag="ACG90W_001399"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0477772.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(12347..13165)
                     /gene="recO"
                     /locus_tag="ACG90W_001400"
     CDS             complement(12347..13165)
                     /gene="recO"
                     /locus_tag="ACG90W_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0477773.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(13308..14207)
                     /gene="era"
                     /locus_tag="ACG90W_001401"
     CDS             complement(13308..14207)
                     /gene="era"
                     /locus_tag="ACG90W_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0477774.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(14222..14617)
                     /locus_tag="ACG90W_001402"
     CDS             complement(14222..14617)
                     /locus_tag="ACG90W_001402"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0477775.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(14601..15077)
                     /gene="ybeY"
                     /locus_tag="ACG90W_001403"
     CDS             complement(14601..15077)
                     /gene="ybeY"
                     /locus_tag="ACG90W_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0477776.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(15106..17298)
                     /locus_tag="ACG90W_001404"
     CDS             complement(15106..17298)
                     /locus_tag="ACG90W_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0477777.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(17322..18293)
                     /locus_tag="ACG90W_001405"
     CDS             complement(17322..18293)
                     /locus_tag="ACG90W_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0477778.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            18998..20377
                     /gene="brnQ"
                     /locus_tag="ACG90W_001406"
     CDS             18998..20377
                     /gene="brnQ"
                     /locus_tag="ACG90W_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0477779.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            20659..>20845
                     /locus_tag="ACG90W_001407"
     CDS             20659..>20845
                     /locus_tag="ACG90W_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0477780.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         5802 a         4393 c         3584 g         7066 t
ORIGIN      
        1 ccttataact cggtataaaa gtcatctcga tattcttttt ttcccaatta aaattgagca
       61 tttgtgtaat tatacttgta attccacctt ttaccgagct gtcattacca atcataagta
      121 ctctttgcat aacgctttac tccttatatt tatatttttt ctattaatac ctctatcgaa
      181 ttaataatcg tttccgtgtt agatacatac ttgttaaaca tccgaactct tgtttcttta
      241 acggcatctt ctaaattttc taattcataa caaggataga taatattcat ctcatcaaat
      301 tgtctaatta actgcagttg gtgatcatcg acatgctcac catacttagc caatcgagga
      361 acagcaatga cccttttacc tttttttacg gccccaatta tagcacctgt acctccatga
      421 gtaatcacca tttctgcttt ttgcatagtt tcagtaaact catccctatt tagaaatgct
      481 ttataggaat aatttagtgg tttataatca ctataaccac tctgtgcaaa tgtactttcg
      541 attattactc ctttttcaat taaatcatca atagctttta acaatctatc aaattgaaat
      601 ttttgtgaac ctaatgttat aaatatcatt tttttctcct attatttaat atatcccacc
      661 caaataaata gcattaggat aaaattgtag catagtttcc cactgaacat aaaattgatc
      721 tgcaaaatga tacatcaatt ttcctgtctg agtagggctg gttacttttg caaacgattc
      781 aatataaatc agtttttttc ccatcatttt tgttattaag cagataggga tcatagctaa
      841 aacacctgta caaatcacga gatccggttt ttcctttata tatataataa ttgatttgaa
      901 taaattaata agtatagtac acaaaaaaaa cttttctttt ctatttactt gtctcatgta
      961 gtacgttttt tcgccttttg actcagctga atattctgtc tgttctgtta taacaaaact
     1021 ctcatacttc tccatcagtg gttttaacat cattaactgt tcataatgcc ccccagagga
     1081 agccgcaaaa catatctttt tgttcatatt aacctctcta tcagtcacgc tgaaaaatat
     1141 ctctagtgta aactttttct ttaatatcat ccaagcaggt atcatatcga ttggcaataa
     1201 tagaatcaca catcgcctta aactgctcta aatcgttgat aaccttactt ccgaaaaaag
     1261 tctctccatc tttcagtgca ggctcataaa tcaccacagt cgcaccttta gcttttacgc
     1321 gcttcataac tccctggatt gaactctgac gaaaattatc actgttgctc ttcatggtca
     1381 gccggtatat accgattaaa actggatttt ccctgttgac atcatactga ttatattcag
     1441 cgtagttgta atagcccgcc atctgaagaa cacgatctgc gatgaaatcc ttacgggtac
     1501 gattagattc aacgatagcc tcaatcaaat ttccaggaac atctgcatag tttgccaaca
     1561 actgcttggt atcttttggc aagcaataac ctccatatcc aaaggatgga ttgttgtagt
     1621 gagttccaat acgaggatcg agacaaacac cattgattat ctgctgagta ttcagcccct
     1681 tcatttcagc ataagtgtct aattcattga aatagctgac tcgcagtgct aaataggtat
     1741 ttgcaaacag ctttactgct tcggcttcag tgaatcccat aaaaagagta tccacgttct
     1801 ccttaattgc acttttctgc agaagtgttg caaaaacatg agcagcattt aacaagcgct
     1861 catcagtcat atcagtacca acaataatac gagatggata cagattatca tataaagcct
     1921 tgctctcgcg cagaaactct ggactaaaaa taatgttctt actccccgtt gtctcacgga
     1981 ctgattcagt gtagccaaca ggaattgtag acttgatgac catgatggta tcaggattca
     2041 cgtccatcac aacctcaata actgactcaa cagcagaagt atcgaaatgc tgagtacgac
     2101 tgtcatagtt agtcggtgta gcaataacaa cgaaatctgc ggatttataa gcgctccttg
     2161 catccaaagt agcagttaag tcaagttctt tttctgtaag atacttttca atatattcat
     2221 cctgaattgg agacttccgt ttattaatca gttcaatctt ttcagggata atgtctaccg
     2281 cggttacctg gtgatgctgc gccaatagtg tggcgatact caaaccaaca taaccggtgc
     2341 cggctactgc gatgttgtac tttttcttca caatatctcc tctttcctct cttaagtatg
     2401 gagaatgtta ctcctacggc tattcatcat gtcaaaataa cacagcttag aatgttgata
     2461 aaataatttt ccagaatgtg ataaaatctc tttcatttat aaaccacaca tctaatttta
     2521 tctattctcc acaagacatg tagagtattc tttaataaca agatacgacc ttactaaata
     2581 cttcaaaaaa ttatcatatt taattcataa atataaatct tcattttaaa tgtatgccca
     2641 aaaatttatc tgtaatgcgt catcaaaatc tagattccaa atctttatca atttttatcc
     2701 actcattcta ccactttagt aatattttct atttataaca ctttcgtcct acattgatcc
     2761 ccccctctga agtacagttt ttactgtctt aaataaaatt ttaaaatcta accccatact
     2821 ccaatttttt atatattccg tgtcaagttt tgctacttct tcaaaatcag taatatcact
     2881 tctaccactt acctgccaca ttccagtaat tcctggctta atagcaattc ttgcacgatg
     2941 atgataatca tattcttcat actctgattt aaggcatggt cgaaatccta taaggctcat
     3001 ctttcctgtt agaacgttga aaaattgagg gaattcatct aaagacgtgg atctaataaa
     3061 ttgaccgatt cctgtctttt ttgttccatc tggaagtatt ttatttccga taattcttgg
     3121 atcaaactcc atcttaaaca taagattatt gctcatctta ttttctttca ttaattctac
     3181 ttttctttct tctgcatcca tatacatact tctaaactta tacatcttga acggtttacc
     3241 attctgacct atacgttctt gtgcaaagaa aattgggcct ggtgaattaa tataaataag
     3301 tggagcaata aagataaaaa taattcccgt tgcaatacat ccaacaatac ctgcaagaat
     3361 atcaattgtt cgttttataa atgcctgttg agttgtcata acattgatac ttgttgtaag
     3421 tactgtatat tctcctatac gaccaataga ttgttttccc aaagcactat ctgatacttt
     3481 tgcaagattc aaatgtacag taaggcccat ttcagagcat cgttcaataa attctttcat
     3541 acacgaagta cttttattca aaataataaa gacttcatct acccatccct gacataagta
     3601 atccgctgca tttttggaat cagaaacaac tgtcactccc tcaacatctt caccaatcat
     3661 gcttttatct atgataatga caccatttat tttatacatt ttatagttat gctctttcac
     3721 attacgaata actacttcag caatactact agatgtaact ataataagtg atttgttccc
     3781 ctcatttttc attttatgtt tcaagtaatg tttccataag aggcgagtta tatatgtaat
     3841 tacaacgtat aaacctccca tcaagaaaag aacgactcta gaataatcat ttccaccctg
     3901 aacactaatg agatacaaac ttcctaacag caccaacata atcgcttgct ttacagaaga
     3961 agcaaattct atatagtacc ctctttttaa tacacctcta tatgcttcaa acatgaaaat
     4021 aactaaaaga tctgccagtt caatgaagac tgccatgttt tgatacattg gaatcacata
     4081 aggattccaa tctacctggc gtaagacata cgatagataa aaagcaagct ggatacacaa
     4141 taaatctaaa ataataaaat caatatgttt aacccatccc cttgaacttt tcttgtacat
     4201 tcctttttac tcccctcttt ttaataaaat aaattgtatt acttgttttc tctcctaaat
     4261 atctttctga aggattattg actcgacaaa ttggcttgtg gatattctta tatatagaac
     4321 atccgtaagc caacttaaaa ctccttaaca aaattttttt actcatattg tagatcaaaa
     4381 caagtatcca agaaatcata ttttatttct tttttctgct tactcaacaa cagctaaaag
     4441 aaaaatcatc ataaacaaat tcaactaagc caacgatccc cttgtgaaat caagtttttt
     4501 cttgatttac gaataaagag ctaaaataaa ccaaacttct tcttttttac ctcttcatag
     4561 actggataac tgatttcatc cccgttgatc agatcctttg cgacttgttt catctgtact
     4621 actttttcct taccaaagtc ctttgcaatc tgctcatagc actcttttaa gtaaaaggtg
     4681 cgcttagaga ccccatgggc atcagaagca gccatctgca cgagattatg tttgaccatc
     4741 gttttggctg ttttctggat gtctttgcca aataccccaa cataacttgg cgcagtgagt
     4801 tgggccaaac atcccatatc taggaacgga agcaaatgat ttgggtcttc gcgaaaatgc
     4861 acgttgcgtt ctggatggac gattactggt gtctttccta gttctaacaa ctcaaaaaaa
     4921 agccgttctg catataacgg cacttccagt gtcggaaatt cgatcagaag atatgtatca
     4981 tctaaatctg taaataatat ttcatctcgg tggatgtctt cgatgagcgt tccggtaata
     5041 cgtacttctt gtccttccaa taacgtcaaa ggcaactgtc ttttctctaa ttcggcttgt
     5101 aacgaagcga ctagagaaat gacttgtgat ttttcattgc tgtattttcc attattgtgg
     5161 tgtggtgtgc ataaaatatg cgtgatgcct tgctgaatcg ctttttcagc cattgcaata
     5221 cttgcttcta agttttctgc cccgtcatcg attcctggta ggatatggca atgtagatcg
     5281 atcatttctc ttgtctcctc tcttcttatt ccttaatttc cgtagtaata accagcatct
     5341 ttgctatgtt ctgctccgtt gtacacaaca cctaagactc gtgcttgtac catctccagc
     5401 agttcttttg ctttagtcaa ggattcttta cgtgacatat tttcacgtac gaccaataaa
     5461 gtgccatccg tttttaaaga catgatctgt gcatctgtta cggctactac tggtggcata
     5521 tcgaatatca ccacgtcaaa taactgtctt gcttcggata ggatctgatc cattcttggc
     5581 gagcttaaca attctgatgg gttgggtggc ttaggtccac taggcaaaat cgacaagttc
     5641 tcgactggcg ttcgttggat cacatctgct acacttcctg atgaactaag tgctgtactt
     5701 aatccactgg cattgttcag ctggaatgtc ttatagacga cgggtttacg taaatctgca
     5761 tcgactagta gaactcgttg acctgattta gcaaaaacga ctgcaatgtt tgctgcagtg
     5821 gtggatttcc cttctccagg acctgatgaa gtcacgacaa tcgttttgat ttgctgacca
     5881 gctgcggagg caaactggat attcgtccgg atcgtacgat attgttcggc gattggtgaa
     5941 gatgggtttg ttaatgtgat caaactgact gcatgagttt gtgctgcttt ttgtttctgt
     6001 gttcgtgcca tttttcttcc tcctagactc ttgagcggct tctgcgtgat ggggctgttt
     6061 tatcgtttgt agcttttggt cgtgcaggag ctgttccttt tttacggata ccagcgttca
     6121 attctttcgc tgacatttct ggaactgttc ctaagattgg gaatcctaat gtatctgtca
     6181 catatttttc atctttcact gtccgatcga gtaattccaa taagaatgcc agacctacac
     6241 ctaccatcat tcctaacatt aagccagtca ttaaattcaa tttattattt ggcgaaacag
     6301 gtgtcgtatt ggcaactgca cctgaaatga tcgagatttt atcaacattc atcacatctt
     6361 ttgcgttctc ttggaaaact tgtgaagttg tgttagcgat ctgttgagca gccactgcat
     6421 tcgtacttat tgcctgaatc gagaacattt gtgaattttc tgcctgatta actgagatgg
     6481 catctttgat ttgttcagca gtcattttca cattgtcttc tgtttctaaa cgatctttga
     6541 cttcattgag cactaaatca ctgacgatca tatctttata cgtattgatc atttgcaggt
     6601 tcgtattgac atcattgaca tttgcagtgt ctgattgtgg cagtgtcacg atcaattgtg
     6661 cttgtgaact gtattttggt gtcaagatga aaaatgttac gattcctgag atggccaagc
     6721 cagcaaacat tgagatgatg attattacca agtgtttgcg taatgtttca aaaatttctt
     6781 tcaagctgat tgtctcttcc attttttctc cactttccta tttattctct agattcattt
     6841 gatcttttaa ttcttttttc acacgatcaa gttcttgatc actgactttt tgataggaga
     6901 tattatcttg catgaagcct tctccttgca gctgatcttg cttaatcgta ccaaatgcct
     6961 cccgataatc taaagcaatt tttttcattt catcaaagtt catgtctgtt ttcatattcg
     7021 actctagagc attcaaaacg gattgatagt tgctgacacc gtttagactc aatacttttt
     7081 tcgctacgcc ttctacgatc tttctttggc gcccttgtct accgtaatca ccatttgggt
     7141 cttcataacg catacgagaa taagataatg cctgatcccc gttcaatgta attttaccga
     7201 ttggaaagtc atagccatct tgtgagaaag tgaggtcatt attgacttcg ataccaccaa
     7261 ctgcatcaac gagttccttt agtcctttca tattgataga gacatagtga tcgattggga
     7321 tgtctacata tttttgtacg gtatccatcg ccatggcagc tccgccgaat gcgtaagcgt
     7381 ggttgatctt gtcttttgtg tcacgaccta cgatgtcgac atacgtatct cgaggaatgc
     7441 tgacgatcgt tgtctgtttg tcttcaggac tgactgtggc aaccatcatc gtatctgatc
     7501 ttccttgatc gacccgacct aaatctccag tatcaatccc catcaataga acagagaaag
     7561 attcttgttt gtccagatca actggtttcg ttcgttctcc ttgggttcgt tccactgatt
     7621 cgtaagtttt ctcaatagaa tgtgagagat ccagatataa ttttgctcct actcctacaa
     7681 ttgctaatac taatacgata agaactgcta aaataatcaa aaatattttt ttccctcttg
     7741 attttcttct tcgtcttgcc atagtttcct ccgtttgcta accttgcttg aatcaacgtt
     7801 ttaactgtca atagcttata ttgctatagc ccatttgctg agcaacagtt tgtgcttttg
     7861 ttgcgtatgc ggctcttatt ttttcattcc ggatctgctc tatttgactg agcgccgtca
     7921 aatattgatc gtaagtagcg gtagctgtta cttgaccgtc tttcagcatc gtatcgaata
     7981 gctgatctag ttgcgtgact tgtgcaattt gggtatcagc gaatcctaga tattgcataa
     8041 cgactgtttt ccaagcacta tccttaaaaa agctcatatt ttcacgtaca tgggcgacat
     8101 ctgcacttgc caacttttct ttgatgatga catcatccac agcttgctgc cagtttgata
     8161 cgtttttagt aaataattta ttgacagccg tctggatctt cagttttgat tctgcatcct
     8221 ctaactgcgt taagactgct ttcttttctt cttggatcgc agcagcttct ttaggtaatt
     8281 ctgattcctt gatgtcataa tcatctgccg aaagtttgat agaagatagt ttatgtgaca
     8341 aatttgaaac tgtttcttct gttatttcag gatttaggaa aattttttct tttgtactgt
     8401 acagcttatc tacttctttt gataaagagg tcacggtgtc tttttgattg gtgatggcag
     8461 attctgcgta attgatccgt tgatgatatt gccgctgatt attgagatgg tatccaaaca
     8521 agcaaatcac aatagcgcct gtgcctaccg ccacagtagc caataacttg acgaacggct
     8581 tttccataga ttcactccta aatattgatg atagataagt aaagatgtga ttcattcaca
     8641 tcagaatgag cgctttttat tttaaaagca taattaaact aatatattca tagaaatatt
     8701 tataaacagg ttttccctat ataatcatac cacatacagt ttttttgact agggaaattc
     8761 ctaatttaaa agtaaataaa acagtaacaa gattataatt aatcgttttc atttttattt
     8821 cctcactttt catgagcgtt ttgaaaattc gaacaaagaa taaaataaca aaaattatta
     8881 aaatagaatt aataacagct atttataaca aaaaaaacag agacacagca cgtttcgtgc
     8941 atgctctctg tttttcaaac aaaagactat tttgtgatga tttgatccaa gcttgctaat
     9001 gacaaagcca tggcagctat ttgacttagt tgtgttagtc ggttcttacg tactgctggt
     9061 tcatcagcca tgaccatcgt ttgatcaaaa taattttcga tcagtggacg aagagcgaca
     9121 agctggtcgt agttttcgtg gatcgtttgg tcagcaaaac ttgctttcat tgcttctact
     9181 gctttgtgta attctttctc tgcgtcgttt tcgaacagct cagggtctac ttctgattgt
     9241 ttttcaagct gcgctttttt cgctaagttc acgacacgtg tcaaagcttc cattgaaggt
     9301 ttgaagtctt catctgcaag atgttgtttg aacaattgtg ctgctgcaaa tacttttgtc
     9361 agatcttttt gttccgcatt caaaacagca tcgatgacat catgacgaat attcttcgtt
     9421 gtcaataatt ggcgcagacg gcctttgata aagtcgatca cttgttgttg cccttctgat
     9481 aaaccaatgc cgaaacgatc cgtatcttta ctgataacat cgctaatcgt ttcttgcaga
     9541 acagacaaag gaaaggccca ttttttcgat tcgatgatcc gaacgacacc gtatgcttgg
     9601 cgacgtaagg cataaggatc gtttgacccg cttggaatca tcccaactgc aaagaaagaa
     9661 aagatgctat ctaatttatc tgcaatcgct aaaacagaac ctactgtaga ctgaggtagc
     9721 tctccttcac tggatatagg catgtaatgc tcacgaatcg ctgtcgctac agcaggtttc
     9781 tcgcctttca gaagagcata tttttcgccc atgatccctt gtagttcagg aaattcccct
     9841 accatgttgg ttactagatc aaatttatag atttcagcag ctcgttgcaa atccatcaat
     9901 tcttcctcag atagtcctac ttgttcgcca atgatacggg caatgacttg tacccgttgc
     9961 attttctcat agatactacc gattttttca tggaaagtaa cagtttttag tttttcaacg
    10021 tattggtcga ttgaagcttt cttgtcttca ttataaaaga attccgcatc ttctaaacga
    10081 gccgtcaata ctttttgatt gcctcgaacc acatcttcca attttacccg atcaccatta
    10141 cgagcagaaa taaaatgagg aagcagcata ccttgatcat tccgtacctc gaaataacgt
    10201 tgatgttctt tcatagaagt gactagtact tcttcaggaa cagaaagata tttttcttcg
    10261 aagtcgccag aaaagacagt tggatattcg actaaattgt tgacttcttc tagtaaatct
    10321 tcgtctagat caataaccca gtgatttttt tctgctaatt cgtttgcttg tgtaacgatc
    10381 atttcttgtc ttttttgcgg agacgcaatg acgaattgtt cattcaattt ttcttcatat
    10441 tcttttgcat gcgcaaatgt cacatcttcg cctaagaaac gatgacctcg agaggctctg
    10501 cctgttttga catccaagat ttcgaatggg attacttcgt catctaacaa tgccacgatc
    10561 cagtgaatag ggcggatata ttcaaaatcg tattctgccc aatgcatcgt tacagggaaa
    10621 gtcaaacttt tgatcacccc tgctaagcct gtcaatactt cttttgcttc cttaccatga
    10681 gtgtatttcg ttacatagac atattccaca ccttttaatt ctctaaaagt gatagcgtct
    10741 gtcgtcagac cttgtccgcg gacaaatcct tccgctgctt tcgtccagtt tccatctgcg
    10801 tccaaagcaa tttttttggc tggtcctttg acttcttctt cactgtccgc ttgtcgttct
    10861 ggaatatttg ttactttgat tgctaaacgg cgcggtgtgg aaaatgtttc gatcgtatca
    10921 taagtcaatt ggttttcatc taaaaatttt gctgtttttt cttctaattg tttcatgctt
    10981 ggtgtgacaa catgggcagg catttcttcc agcccgattt caagtaataa gtcttttgcc
    11041 atcttattca gcctccttca cggtttctgg atttgctgtg tttttattca acaaagggaa
    11101 tcctaatttt tcgcgttctg ccacaaagat tttggctaca gcacgagcca tgttgcggat
    11161 gcgagaaaga taaccggcac gctctgttac ggatactgca ccgcgggcgt ctaataagtt
    11221 gaatgtatgg ctacatttca agatataatc atacgccgga tgaacaagat tttcatcgat
    11281 acagcgttta gcttcttttt caaaacgatc aaagttgctt agtagcaagt cttggtcgct
    11341 tacttcaaaa gaatatttgg aatgttcata ttctggttgt ttgaagatct cgccatattt
    11401 cacgccatca gcccattcta aatcatagac actttcaact tcttggatat aagaagcaag
    11461 acgttctaat ccataagtga tctcagaagt tactggttta caaggcagtc caccaacttg
    11521 ctggaagtac gtgaattgtg tgatttccat tccgtcaagc catacttccc aacccacacc
    11581 ggcacagccc atggatgggt tctcccagtt gtcttcaaca aaacggatgt cgtgttctaa
    11641 tggatcgatt cctaaaaggc gcaagctttc taaataaagc tcttgaatat tctcaggtga
    11701 aggtttcatc accacttgga attgatgatg ctgatacaaa cggtttgggt tttcaccata
    11761 acgtccatca gctggtcggc gagatggctc tacgtaagca gcattccatg gttctggtcc
    11821 gattgcccgt aaaaaggtat atgggctcat cgtacctgct cctttttctg tgtcataagc
    11881 ttgcatcagc atgcagccgt ttgatgacca aaatttttgt aatgttaaaa tcatatcttg
    11941 gacggtcaat tttttactca tctgttttgc ccctttcaat tttaaagcgt tgctgtcaaa
    12001 taagaataca ggaaagaaaa cgacaaaaaa tccctatgtc tgattacaga catagggacg
    12061 aataattcgc ggttccaccc tacttccgaa taaagattcg gcagcttcat ttatcgtact
    12121 cgcaagtgcc tcttcaaaaa tcgtttgggt ccggctccca ctctcccgaa ctcgctaaac
    12181 cacgtgtttt ttactgatct tggtcaatgt acgtattcat tttgcagttt tcttgtttct
    12241 ataatagatc ctatcactga acgatctatt taatctatgg ttaataatag acggaactcc
    12301 ctgtgtttgt caaatggttt tgattttttg ttgtatgttt tatttactat tcttcttttt
    12361 caggtgtctc tttttctttt tcagacatgt ctttctctga cgtctttaat acgtgtgccc
    12421 aagacttcat ttgattgatg aactttttgc ttttcaaatg gataccgaca tattcatcat
    12481 acagctggtc gatgagttca cggattgctt gtttggtttc ttctttcaga tggattccac
    12541 taattttatc ataagaaata gctgaaaaca tccgaacgaa ataaacagct cgaggatctg
    12601 catgataacg gtgatcgtcc atcgaccaat gtctttcgca tatcacacca ccatatttgg
    12661 aagaatagtc aaattttcct tttgtttccc cgcatacgac gcagtgtgtc cagattggtg
    12721 caattccgaa gcgttgcagt atttggactt caaaaatatt cgtgatgatt tctgcatcgt
    12781 ctccttggtc catccgttgc agtgcttgtt gcgtaaactg gaacaaatgc gggtcgtact
    12841 gatgatcttc gattgccgca tctactagat tcaataaata tgtgccatag ccgctcaaaa
    12901 agatgtcttc ttggatgtgt cgaaacgcat ggacatcttt gacactgctt aaaaatgaca
    12961 aaccttcact tcgaaaattc ccgatataaa ctgcttcagt aaaagggagc aaagccggag
    13021 ctaagggctg attttgccga tggatctctt tgatataaaa catcattttg ccagaagatt
    13081 ctgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat aaaatcaagc
    13141 cttttgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct ggaaaagcag
    13201 attctgccga tccagatcat ttatgttgcg atcttccgca gcttttctca acacgcggat
    13261 ttttattcag tttatttcta tacctaattt acaagaagct cagaatatca gtattcgtct
    13321 tttcggtagc cgaagtcttg cagatagact tttttgtctc gccagtcttt ttgaactttg
    13381 acccaaagtt ctagaaagac tttgtctcct aaaagatttt cgatatcttt tcttgctttc
    13441 gttcctactt gcttcagcat ttttccgcct ttgccgataa tgatcccttt ttggctatct
    13501 ctttcgacaa taatcgtagc ctggatatgg attttatcat tctcgtttcg cttcatcgaa
    13561 tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa gactttctca
    13621 cggatcaatt cagaaacgat gaagtattca ggatgatcag tcacttgatc atctggaaaa
    13681 tattgtgggc cctctggcat ttcactgact aatacatcca tcaaacgttc gacgttgttt
    13741 ccttctgtag cagagattgg aatcacttgt gcaaaaggca tctgcgttga ataatcttcg
    13801 atgattccca acaactcgtc tggatggatt ttgtcgattt tattgataat cagataaaca
    13861 ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccgacc gcgtttttga
    13921 tcagcactaa tcataaataa gaccgcgtcc acttctttca acgcactgta agctgcttct
    13981 accataaaat ctcctaaacg atgctttggt ttatggattc ctggtgtatc aataaaaatc
    14041 aactgagctt ctggtgtagt gtatactcct tggattttat ttctagttgt ctgtgccttg
    14101 tcactcatga tggcaatttt ttgacccacg attcgattca ataatgtgga tttccctaca
    14161 tttgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata tgttctcctt
    14221 tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca acgatgacgg
    14281 taaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt ttcccaatcg
    14341 gatgaaaatg aaaatccgta aacatgtcca cgacattttc aaaaacagta ttgatgatct
    14401 ctactatcca tacgagaaaa acagctaaaa gcagccataa ccattctatc cgatcaagtt
    14461 ggaaaatcac gcccataaac aaagcgagga ttccgaacgc aacatgtttc ctcatgttgc
    14521 gttcttcctc aaaaacagtt ttgactcctt ggacggcaaa ttccacggac atcaggaaat
    14581 gtttgttttt ctctacgttt ttatctttta agtccatagg catctaagat ctctttctgt
    14641 aaaccaaaca tttctttctc atcctctggt gtcatatggt catacccgtt gatatgcaag
    14701 aatccatgaa ccgctaaaaa gccaagttcc cgatcatacg aatggccgta agcttctgct
    14761 tgctctgcag cacgttcagt agagatcatg atgtctccca gtacacgagg caatggattc
    14821 tctccatctt caaaaatcac tgggatttcg tcttcgccct cttcttccaa ggcaaaactg
    14881 atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg gattgctgca
    14941 ttgtccatga aagtaacaga catttctgtt tcttctggca actctaaata tgatgctgca
    15001 aattgcagca agtcgtcgat ttctttcatt ttctcttctg agacttggtt tgtttcatcc
    15061 atgaaggtaa tatccattat acatcgcttc tttctattct tcttgctatt cttcttgttc
    15121 ttcttttatc ttctcaactt ccgatttcat tttaggatat ttgatccgag aatggaaggt
    15181 actgcttaaa ccgctgatca atgatttttc tactttacgg atttctttta acgtcaatcc
    15241 actgtcgtcc aattgtccat cggaaatacg ctcttcaata agattatgga caaaactttc
    15301 gattttttcg ctcgttggat ggtccattgc ccgtactgcc gcttcacaac tgtcagctat
    15361 attgacgatt cctgcttcac ggctttgagg ttttggtcca ggataacgga attgctcttc
    15421 cgtaacttcc gggttgcgtt cctttgcctt aacgtagaaa taacgcatca gcgtggttcc
    15481 atgatgctgt ctacaaatat cgatcaccat ttgcggcata ttataatctt ccaatatttt
    15541 ggcaccatct gtcacatgtc caaagataat atgtttactg tcttctggca ataagaaatt
    15601 gtgcggattt tccgctcctg aaggcaagtt ctcaacgaaa aagctggcgt gtttgatctt
    15661 gccgatatca tgataatagc aagctactcg agttagtaaa gaacgtccac cgatttctgc
    15721 tacagcatta gcacttagat tggctaccat catgctatgg tgatacgtac ctggtgcttc
    15781 ttccagcaac tgtttcaata aaggatgatt gggattgctg agttcattta atacgatcac
    15841 actatcatca ttgaccatca attcaatata ggggtgcaga cctatcgtca gcaagaatga
    15901 aaacagtgca cctgtgaatc cgcagactag cgttaaccaa gtcgttgcat ccccaaagct
    15961 cattccttgg tagataatca aaatgacatc catcatcaca ggaaaaataa tgacccacat
    16021 agcagcggat aacccttgtt ctgagatccg tttgcgtctg acgatcgttg ccatgagacc
    16081 agaaaataca taggacatca aaataactgt cagaaagttc gtgccaatcg aatcataaaa
    16141 aatgaaaagt gcggatactg cttgaaaaat accagccatg atccctgctc ttcgattcaa
    16201 gaaaaagctt aaaacaagtg gagcaaaagc cgcggggtaa aacagcggaa tataagcagc
    16261 ttgttctgtt tggaagagtt ggaaaaattt cattagtacg acgcttactg acattgccgt
    16321 cacgtagaaa agtacgtatt ttgttctttc tcctgcattt tggaattgaa gtgaattata
    16381 aatcagtaca ccgatttgaa gcaaaacagc taaaaccatg gcaatgagtg ggaaaataga
    16441 agtcgtttga ttcgtcaatc ctagtaattc caattttttc atcgctgaag catcaatttg
    16501 atttccttcg cgaacaataa tttctccttg gaagatcatc actggttgta cagcttctcg
    16561 tgcagattgt tttaattctt ctgtcttttt ctcattcaat acatcattaa ctacgatgcc
    16621 ttgatccact aagtagcgaa tcatttgttg ctgctctgtt gtgacattca aatattgaat
    16681 ctgatcttct gcttcttgtt taaaagcttc caattcgctt tcacgaactt gtttactcat
    16741 ctgttcgtca atgagcttca aactttcatc cctaactgtg tgaatttccg tttcagtcat
    16801 ttcaaatatc gtcgaataaa aattgttcgg cagtttttgg taaaacgcaa cgttttctgc
    16861 attcacgttt tcaaaatttt tcttcagtgt agcaatccgc tcttctgtag taggtttcgg
    16921 gatattttca tcttccttcg ccttatctac cttgccatta taagatttgt tgatggtatc
    16981 attcgtctta tcgatcaatt cgaataattg cttgatccga ttgttctggt catctgccag
    17041 atctttttga taagtatatt ctggagtcac tgcttctgct gccagttttc ttctttgttc
    17101 ggtttcttcc gtgttctcaa tcgttttatt cgcacggata ctctcttctg ctacttggcc
    17161 ttctctgaaa tcaatggcct tttgtttgac actactaaat gtaacgccgc agacaatgat
    17221 aaaaaacaag aacagaagga aaggcaatgc tgcctttccg agtttttctt gtagtttctg
    17281 cagtgtttta ttcaacatgt gcttatcctc ctaagcaaag cttattcttg atgaagatcc
    17341 gccttttcat aagctttgat gatttcggca actacagggt gccgaacaac gtcacttgct
    17401 tcaaaattaa caaaatcgat ttttttgatt tgctttaatg ttcgttctgc atgaacaaga
    17461 ccacttgtcg tcccacgagg caagtctatc tgacttgtat ccccattgac aatcattttt
    17521 gattgataac cgagacgggt caagaacatt ttcatctgag cgactgtcgt gttttgtgct
    17581 tcgtccaaga taacgaatgc atcatccaat gtccgccccc gcatgtaagc taaaggtgcg
    17641 atttcgatca cgcctcgttc catcaatcgg ttcgtatgat ccaatccaaa aatttggtat
    17701 aaagcatcat aaacaggccg cagataagga tccacttttt ctttcagatc cccgggtaga
    17761 aagcctaggt tttcccctgc ctccacagcg ggccgcgtca aaatgatttt ttgcacttcg
    17821 ccttttttta gggcagcaat tgctaatact acggcaagaa acgttttccc tgttccggct
    17881 ggccctacac caaatacgat gtcatgtttg gctaccgatt caacgtattt cttttgaccg
    17941 ctgtttttta cccgtatagg ttttccatta cggtctttga cgatctcttc ctcatacatc
    18001 tcaacaaaat aatctaatgt acctttttgc cccattttca atgccgtcac aacatctggc
    18061 gtacttacgt ggattcctcg tttgatcaat tcctgtaatg tccgaatgac tgaggcagcc
    18121 aaggaaatct gggattgctc accaatgatc tgaatgattt ctccacgtgt atggatggtt
    18181 gtttccgttg tttcctcgat cagttttata tgcttgtcgt gtgcacctaa aagcatgctg
    18241 acatcatcag cagctgtcaa tctaatctct aaagaactag actcttccgt caaaaataga
    18301 catccccttt ttttcgattc attctttcct attttaccat actcagaaga aactatatag
    18361 caaataagaa aaggttgtaa aagagtcgct taaccatacg taccaagaaa cgttaaaaaa
    18421 tagccgtttc cattcgctaa aggtctcgtt ttgataaaaa gaagctgaga atgtaagtta
    18481 tatgaaagtg gagaacaaaa tatttcgttt cattcaacaa gtattctcta ggtatttttc
    18541 ctgtttcaaa aaaagaaaac aaaatagtta aaatattctt gcatttcaaa aaaaacaggt
    18601 gtattattac gtcaacaaat aaacatacag caaagcgtcg aggaaaacag taggctattc
    18661 aacgatttat tcccagtgag tcatgtaagt gagaaatgac aataaatcga atagacgaac
    18721 atcatttccg agtcaatgtc gaaaacgtag atcattgtgg ctgcctccat tatcaggcta
    18781 gcggataact ttatcaaaaa gcgtccgtga atgaagaagc ttttttaagc gaaaaaaggt
    18841 ggtaccgttc tagaatctag agcccttttc ccaagtggaa aagggctttt ttttatttta
    18901 aggtggtggt tttcatggaa tctgatatta gtggcattac taattgctgc aacgtttata
    18961 tcgtcaatca aaatgataat tattggagga aaaatttatg gaagaaacaa aaacttattc
    19021 atggaaacaa ctattgatgg tcagttcact tatcttcggg atgttcttcg ggtcaggtaa
    19081 tctaatcttt cctgtccact taggtcaaat ggctggcgca aactgggtca gtgctggcgt
    19141 aggatttgct atctcaggtg ctcttttccc attactagct atcttagctg tagtagtgac
    19201 aaaaagtgat ggtctgtatg acttagctaa acctgtcgga aaatggtatg ctgccttgtt
    19261 cttaatcctt gttcatttga cgatcggacc atttttcggt acaccacgga cagctgctac
    19321 tgcttttgaa atggctgcta aaccattttt gcctgaaaaa tataattcat taggtatgtt
    19381 tttattctct gctcttttct ttttacttgc ttatttcctt actgtccatc caacacggtt
    19441 ggtcaaatac gtcggaaagt atctaaactc cttgtttctt gcattattag caatcatttt
    19501 tgtcgtttct ttattccatc caatgggaaa tctaaatcaa tctgctgcag atacttatca
    19561 aacaaacgct ttaacaaatg gtgtcctaga aggttataac acggttgatg cagtagcttt
    19621 gcttgcctta agtgtcactt ttgtccatgc agtccgtgac cttggtttca aagataaaaa
    19681 agtcacagaa ttaacggcaa aagctggtag cctgagcgtt gcgctagaag tcatgatcta
    19741 ttttggactt gttcttctcg gtgcgatgag tttgaataag atgtctttat cagaaaatgg
    19801 cggaactgca ctttctcaga tcactaattt ttatttaggt aacttcggcg ctagcttctt
    19861 aggagtgatg gtcacattag gtgtcttcac taccgctatg ggactcgttg tttcttttgc
    19921 gcaagatttc cataaactgt tccctaaagt cagttatatg acatggctgc ggttaacgac
    19981 tttcgtctct tttgtcgttg caaatgctgg tttagacaat atcatccaat ggtctttacc
    20041 agtattgatg cttttgtatc cattgtcgct tgcgttgatc ttgctttcct taacagcaaa
    20101 atttttccaa aagacaccat ttgtctatca agtaacgatg ttgtttgctg ctgttcctgc
    20161 cgtattggat atgctggcaa attcgcctgc tttagtgagt caacaaagag ttgtagcaag
    20221 catgctcgaa ttttatcatc atcacgttcc ttttgctgca ttaggacttg gctggatggt
    20281 cccaacgtta cttggttacg ctggcagcct actgttttat tacgcctatc gcctatcagg
    20341 gtataaacaa gaagcaaatg aactaccaga agaataacca aatcaaacac aatttaacat
    20401 aagaaatatt aaacaggaaa aaaccagcta gatgaaagaa tcctattctt tcgtctagct
    20461 attttttagt tctctttttt acgcgcacaa aaaaactgcg gacaatgatt gtccgcagtt
    20521 taaatgggct ctataatatc tagtgttggt gacgccttct gttgccaata ctttttcttt
    20581 tttctaataa acgcaaacag acaccttgaa atcctttaga ataaagtcga cgaaaaccat
    20641 tcaaaggagt gtttcaagat gtcctattca gagtttacca aagagttact agatattcta
    20701 gacctaaatc ttatctttca tgaagatgcc tttcgcaaag agcgaatcaa tgacgaaaca
    20761 tgttttgttt ttgatggcac attgacctat caaccagagg aatgcttcca ttgtcactat
    20821 caaaataagc aaacgatcat taaat
//
LOCUS       DAZKOK010000045        20823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000045 DAZKOK010000000
VERSION     DAZKOK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.045"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG90W_001408"
     CDS             591..1280
                     /locus_tag="ACG90W_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0477781.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG90W_001409"
     CDS             1267..2475
                     /locus_tag="ACG90W_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0477782.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG90W_001410"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG90W_001410"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0477783.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG90W_001411"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG90W_001411"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0477784.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG90W_001412"
     CDS             complement(6578..7273)
                     /locus_tag="ACG90W_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0477785.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG90W_001413"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG90W_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0477786.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG90W_001414"
     CDS             complement(8472..8663)
                     /locus_tag="ACG90W_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0477787.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG90W_001415"
     CDS             complement(8684..9574)
                     /locus_tag="ACG90W_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0477788.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG90W_001416"
     CDS             complement(9561..10328)
                     /locus_tag="ACG90W_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0477789.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG90W_001417"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG90W_001417"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0477790.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG90W_001418"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG90W_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0477791.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG90W_001419"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG90W_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0477792.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG90W_001420"
     CDS             14899..15282
                     /locus_tag="ACG90W_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477793.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG90W_001421"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG90W_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0477794.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG90W_001422"
     CDS             complement(16226..17044)
                     /locus_tag="ACG90W_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0477795.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG90W_001423"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG90W_001423"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0477796.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG90W_001424"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG90W_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0477797.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG90W_001425"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG90W_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0477798.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..>20823
                     /gene="dnaN"
                     /locus_tag="ACG90W_001426"
     CDS             19704..>20823
                     /gene="dnaN"
                     /locus_tag="ACG90W_001426"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0477799.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPV"
BASE COUNT         5938 a         4249 c         3824 g         6812 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tac
//
LOCUS       DAZKOK010000046        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000046 DAZKOK010000000
VERSION     DAZKOK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.046"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG90W_001427"
     CDS             291..1430
                     /locus_tag="ACG90W_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0477800.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG90W_001428"
     CDS             complement(1497..2072)
                     /locus_tag="ACG90W_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0477801.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG90W_001429"
     CDS             complement(2077..3051)
                     /locus_tag="ACG90W_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0477802.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG90W_001430"
     CDS             3334..4302
                     /locus_tag="ACG90W_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0477803.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG90W_001431"
     CDS             4415..4693
                     /locus_tag="ACG90W_001431"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477804.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG90W_001432"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG90W_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0477805.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG90W_001433"
     CDS             5150..5905
                     /locus_tag="ACG90W_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0477806.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG90W_001434"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG90W_001434"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0477807.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG90W_001435"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG90W_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0477808.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG90W_001436"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG90W_001436"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0477809.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG90W_001437"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG90W_001437"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0477810.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG90W_001438"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG90W_001438"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0477811.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG90W_001439"
     CDS             10642..11184
                     /locus_tag="ACG90W_001439"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0477812.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG90W_001440"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG90W_001440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0477813.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG90W_001441"
     CDS             12777..13679
                     /locus_tag="ACG90W_001441"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0477814.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG90W_001442"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG90W_001442"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0477815.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG90W_001443"
     CDS             15122..15544
                     /locus_tag="ACG90W_001443"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0477816.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG90W_001444"
     CDS             16013..16243
                     /locus_tag="ACG90W_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0477817.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG90W_001445"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG90W_001445"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0477818.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG90W_001446"
     CDS             17654..17830
                     /locus_tag="ACG90W_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0477819.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG90W_001447"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG90W_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0477820.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG90W_001448"
     CDS             18325..18708
                     /locus_tag="ACG90W_001448"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0477821.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG90W_001449"
     CDS             18877..19761
                     /locus_tag="ACG90W_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0477822.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG90W_001450"
     CDS             19875..20111
                     /locus_tag="ACG90W_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0477823.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG90W_001451"
     tRNA            20199..20279
                     /locus_tag="ACG90W_001451"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG90W_001452"
     tRNA            20286..20357
                     /locus_tag="ACG90W_001452"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKOK010000047        20335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000047 DAZKOK010000000
VERSION     DAZKOK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.047"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..1608
                     /locus_tag="ACG90W_001453"
     CDS             106..1608
                     /locus_tag="ACG90W_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0477824.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            1610..2347
                     /locus_tag="ACG90W_001454"
     CDS             1610..2347
                     /locus_tag="ACG90W_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477825.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(2518..3711)
                     /locus_tag="ACG90W_001455"
     CDS             complement(2518..3711)
                     /locus_tag="ACG90W_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0477826.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(3790..3996)
                     /locus_tag="ACG90W_001456"
     CDS             complement(3790..3996)
                     /locus_tag="ACG90W_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0477827.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(3989..5131)
                     /locus_tag="ACG90W_001457"
     CDS             complement(3989..5131)
                     /locus_tag="ACG90W_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0477828.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            5643..6278
                     /locus_tag="ACG90W_001458"
     CDS             5643..6278
                     /locus_tag="ACG90W_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0477829.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            6432..6509
                     /locus_tag="ACG90W_001459"
                     /pseudo
     CDS             6432..6509
                     /locus_tag="ACG90W_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            6823..6870
                     /locus_tag="ACG90W_001460"
                     /pseudo
     CDS             6823..6870
                     /locus_tag="ACG90W_001460"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6886..8805
                     /gene="tet(M)"
                     /locus_tag="ACG90W_001461"
     CDS             6886..8805
                     /gene="tet(M)"
                     /locus_tag="ACG90W_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0477830.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            8924..9091
                     /locus_tag="ACG90W_001462"
     CDS             8924..9091
                     /locus_tag="ACG90W_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0477831.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(9151..9504)
                     /locus_tag="ACG90W_001463"
     CDS             complement(9151..9504)
                     /locus_tag="ACG90W_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477832.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            10008..10427
                     /locus_tag="ACG90W_001464"
     CDS             10008..10427
                     /locus_tag="ACG90W_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0477833.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            10453..10659
                     /locus_tag="ACG90W_001465"
     CDS             10453..10659
                     /locus_tag="ACG90W_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477834.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(10887..11060)
                     /locus_tag="ACG90W_001466"
     CDS             complement(10887..11060)
                     /locus_tag="ACG90W_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477835.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            11072..11701
                     /locus_tag="ACG90W_001467"
     CDS             11072..11701
                     /locus_tag="ACG90W_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477836.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(11783..12949)
                     /gene="dnaJ"
                     /locus_tag="ACG90W_001468"
     CDS             complement(11783..12949)
                     /gene="dnaJ"
                     /locus_tag="ACG90W_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0477837.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(13100..14929)
                     /gene="dnaK"
                     /locus_tag="ACG90W_001469"
     CDS             complement(13100..14929)
                     /gene="dnaK"
                     /locus_tag="ACG90W_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0477838.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(14980..15543)
                     /gene="grpE"
                     /locus_tag="ACG90W_001470"
     CDS             complement(14980..15543)
                     /gene="grpE"
                     /locus_tag="ACG90W_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0477839.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(15637..16680)
                     /gene="hrcA"
                     /locus_tag="ACG90W_001471"
     CDS             complement(15637..16680)
                     /gene="hrcA"
                     /locus_tag="ACG90W_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0477840.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(16914..18079)
                     /gene="hemW"
                     /locus_tag="ACG90W_001472"
                     /pseudo
     CDS             complement(16914..18079)
                     /gene="hemW"
                     /locus_tag="ACG90W_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(18095..18322)
                     /locus_tag="ACG90W_001473"
     CDS             complement(18095..18322)
                     /locus_tag="ACG90W_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0477841.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            18806..19413
                     /locus_tag="ACG90W_001474"
                     /pseudo
     CDS             18806..19413
                     /locus_tag="ACG90W_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            19586..20299
                     /locus_tag="ACG90W_001475"
     CDS             19586..20299
                     /locus_tag="ACG90W_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477842.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         6023 a         3929 c         3777 g         6606 t
ORIGIN      
        1 atgttagaaa ctcgcatttt tttcattttt tcgagcttac agtcttgtct gattcagcac
       61 ttttcgccta taataataaa atattggaaa tttaggaggt tgattatgaa aagaaagcat
      121 ttatttcact catttgtttt cttaatgaca ggacttgtga cacttgcgtt tggtcatgtt
      181 gcacaagctg acaacactac aacggcagag tcaagcacaa gcgagacaat gcaaaaaata
      241 gagcccaaaa aagacgtata cgtaattgcc agtgactctg cttttgctcc atttgaattt
      301 caaaacgcaa agggcgagta cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa
      361 ctgcaaggat ttaacatccg aatggactac cgtggtttct ctgctgcttt gcaagctttg
      421 gaatcaggac aagcagacgg aatgattgcc ggaatgactg taacagatga acgaaagaaa
      481 agctatgatt tttctgattc ttactttgat agcgggatcc aattagctgt caaaaaagga
      541 gatacttcga tcaagtctta tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt
      601 accgaaagtg ctgaattttt aagcgaccat gaatctgaat atggctattc tatcaaatat
      661 tttgatgctg ctgatcaatt gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg
      721 gatgattatc cggtaatcgg ttatgcgatc aaacaaggac aagaattgga aacaccgatc
      781 aaaaaagaat ctggcggaaa atatgcattt gccgttaaaa aaggacaaaa tcctgagctt
      841 cttaatatgt tcaatgaagg attgaaagaa ttaaaaagaa caggcgaata cgataagatt
      901 attaaagaat atgtagcgga tggttcagaa acttccagtg agtcttccac tgctgatgaa
      961 tcaacatttc ttggacttat ccaaaacaac tatcaagcat tgttgaaagg actttggaga
     1021 acaattctcc taactttgat ctcctttgct ttggctatag taatcggtgt gatttttgga
     1081 ttattcagtg tagcaccaat acgcgctctt cgcacaattg ctagtattta tgtcgatatc
     1141 atccgaggaa ttccaatgat ggttcttgcg ttctttattt tctttgggtt gcctggtatc
     1201 gttggattta cgattcctga cttccttgct ggtgttatca ccttgactct taacgcaagt
     1261 gcttatatcg cagagattgt tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa
     1321 gcttccagaa gtttaggtct ttcttataat cggacaatgc aaaaaatcat tttgccgcaa
     1381 gcgatacgca tcatgatccc atcattcgtc aatcagttcg ttatctcatt aaaagacaca
     1441 acgatcattt ccgcaatcgg tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca
     1501 cgtacaacac aaagtacgta tgtttacttg atcattgcga tcatgtactt gattttgatc
     1561 acagcattga caaaattagc aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa
     1621 aatcttagtt gagcatttag tcaaaaaata cggcgataac acagtattaa acgacatcaa
     1681 tgtatccatc aacgaaggag atgtcgtctg tatcattggc ccttcaggtt caggaaaaag
     1741 tacattcttg cgttgcttga atcgtttaga agaaccttct gacggtgaaa ttattattga
     1801 cggcgcccac ttgatggata aaagcacaga tatcaacttg gtgagacaac atatcggaat
     1861 ggtattccaa cactttaacc tattccctca tctttctatt ttggaaaaca tcattttagc
     1921 accgatggac ctgaagaaag aatctagaga agaagcagag aaaaaagcaa tcaaattact
     1981 gcagacagtt ggtctagaag ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca
     2041 aaaacagcgt gtggctattg ctcgtgctct agcgatgaac cctgacatca tgctttttga
     2101 tgagcctact tctgcactcg atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa
     2161 gttagcagaa caaggtatga cgatggttat cgttacgcac gaaatggggt tcgctaaaga
     2221 agtggccaat cgcgtcatgt tcattgacga cggaaacttt ttagaagatg gaacgcctca
     2281 acaaatcttt gaaaatccgc aaaatgaacg gacaaaagat ttcttgaata aagtattaaa
     2341 tatttaaaat aaattatata tgtctttttg actatctttt tatatcttct taaatgtccg
     2401 caaagcctta ttttataggc tctacgggca ttttgctttt gtggtaaacc tcacatatct
     2461 aggtctatct tcttatattt tcactatcaa gcgtggttaa aatcgtggta aatacttcta
     2521 tgcaattaga cgctgaacct ctgattttgc ggtatctatg gacgcatgag cataccagtt
     2581 cattgtgata ctgatatttg aatgccccat gatatactgt aaatcttttg ggttcatgtt
     2641 cttgcttgcc agtcttgtgc agaatgtatg gcgtagcgta tgcggtgtga tatgtggcaa
     2701 tggattgtcc ttgtggtgct tgttgtattt ctttaccata cggataaata aggcgttata
     2761 atcaatcgca actttgggct tgcccttatg attgacaaat aggaagttgc cccgtccgtc
     2821 tatcacaaat ggttctgcct ttggacgttt tttcataacc tgttgaaatg cctgtatcgt
     2881 ttctttgctt aatggtactt gccttattcc gctttttgtt ttaggcgttt caatataata
     2941 gccctgttcc ttgctcttta gtaactggtg gtcgataatc acaacttcat tcttgaaatc
     3001 aatgtcggct actgtcagtc cgcacagttc cgaaatacga agtcctgtct ttaacagtat
     3061 cagcacatca tcataatgct tgtgatacac attgtccgtc ttgatgaatg agagtaaggc
     3121 ttgttcctgt tcctctgtca atgcgacttt ctttttggta tcattttcta ggacttcact
     3181 caacttgaaa tcaaagggat ttttcctcac ataatcatct tgaatggcta tgtagaatga
     3241 cgcttttaac gagcgtttat ggttgttaat ggtattgtag gaaaagcctt tgtctttcat
     3301 gcgtaacgcc cattccttag cgtcagaggg tttgattgta tcaatgctcc tagcacctaa
     3361 cttgtcctct ttcaataacc gcatgagttg ttcccgttgt ttctgtgtgc ttttcttcac
     3421 atttgccctc tgtgcgttct gtttggcata aagttggcaa agcgtcattt tcttgccagt
     3481 gctgtcgata ccgtcctcaa tatcccgtct tatctgctgt tccagttctc gaagtgaggg
     3541 tttttcccgt tttccctttg gtgtcgggtc tgtgggtgtc aatctccaag catacacata
     3601 ttttgtgttt ccaaatgcgt ctacatattt atataagtat tttctgtctg ttcgttggct
     3661 ctctccagta tgcaggatac gacctttgtt atcccgtctt ttttctttca tggtgtctgc
     3721 tcctttcctt gttggaaaga gccttgatat gacttgtgtt catcataaca catacaaggc
     3781 tcatttgcat tagatagcgt ccaatttatc aatgacctgt tcaaactgtc ggcgtttaat
     3841 ctgtatgcgg ttgccattca taatgagcca gccagcgtcc ttgttttcct ctgccaatcg
     3901 tcttaacttg ttttctccga tacggaaata ctttgacgct tcttcaatgg taagggtgta
     3961 tttttcccat acgggaatat cctgtgcatt actcataaaa caccccctca ctttcgatta
     4021 cgtctgctat gtccgtacac atctgttcca caagcatttc ctgcttttca gagaggtggg
     4081 tacattcatc aatcattttc atgtagtttg agcctgtcag cctgtcgatt tcctctatca
     4141 tcacgattga cggcatgacc tgtctttcca gccattgcag agtgcgttcc atattgacag
     4201 gtacggggtt catttcaaaa ggtaaactgt catgggaaat gaatatctcc cacagcggat
     4261 agtcgggaaa ttgcacaaaa tcagtaataa ggtaaaacac cagcccatga gggttatatg
     4321 tcaataacaa ttcctcaact gcctgtactg cttttgtacc acgcagacga atttcaaaac
     4381 gattgataat atccgtatgt ttctttttgg ttgcctgttc cttttgtttt tcatacaggc
     4441 agaagtattt tgtgcttgcc tttgaaccga tataaagggt gcttgctaaa ttacggtttt
     4501 ttccgcttag ttttccaccc tgcacatttt cataattttt acagcgacaa tccgcaccgc
     4561 catttctgta tttttcagac aaaacgggaa tatccaacag accacacata tcattgattg
     4621 ccaagtcaaa acgtcggata acaccgtcac agtcaagaca gcggtttaaa aatgaatacc
     4681 aatctctgtt ctgtgcctgc aagacatatt ccatgttgcg tgagcctgcc ccttttaatt
     4741 ccaagaatac gcccatgtgt tcgtcatcag aagccatgac cttgatttct ccgtaggcgt
     4801 aatgctcttt atagccataa tacccatagt cataatggat aaaataatct gctttcatgc
     4861 caagcacctt tcggataatt tccaatgcgt cagtcgttgg ataacggaca gacagataat
     4921 caacgagcag ataaaaaggc ttatcgcaga tataataatt cagacaacgc aggataatat
     4981 tttgtgtttc cttgctggcg ttggcttttc cgttctcaaa gcggttgata ctatatcttg
     5041 atacatgggt aagacttgca agttctcctt gtgaaagacc gcttgcaaga cgtttgttct
     5101 tcatttcaag tgcaaattct aaattgttca ttgtattcct ccaatcccac aatagccttg
     5161 aaatacctgt aaacctctat tcaaaaagtg tgatatgcac tatcttctta aaacttctca
     5221 ttactactca caactttggg attgtatggt ttttgacttg tcttttgtga aatagtgccc
     5281 ccctgttaga taacgggggc acagacaagg ctcgcaggct cgccagccga caaagcattt
     5341 cttccccttg acacaaggtg tcatggcggt aagcctgaga ggcttccgcc accgtgtcag
     5401 aaatactttg tcagcaagtt ctgttttgcc ttttctaaca agttccattt caaccgatat
     5461 tcatatctac ggatttaatc agataggtat ttctttttga atgtttttat atttcattct
     5521 attgtgctta ttcgattaca agaacatagt aattcttata tgcttatttg tcaatatctt
     5581 tttttgcctt tacaaatcat attaagcatg atagacttat aatagcgaag atataacgag
     5641 atatggaggg ttatgcaatg aaaataagcc atttaggaaa taatatacag accataagaa
     5701 aatttagggg aatgaaacaa caggaacttg cggacaaaat cggtatcaat atgcagagcc
     5761 tttccaagat tgaaagaggg ttgaactatc ctgcctatga aacgctggaa aagataatgg
     5821 aagtgctgga cgttacgccc aatgaattgt tgtcgggaga atggaagtat gtcaatcaat
     5881 ctgaaaagga agtctgtcag tttttaagag ccgaagaacg tctaaatgca gaactgaaac
     5941 atggacacta tgataacttc tttgacagcg aggaagaatg gctggaatat gagttagaaa
     6001 agttacggga atatattaca gactatatca acgggaaaag cattgaagcg tccgaccttt
     6061 acccaatcaa agaatttatc cagcatttga agtttcaaaa attattagac cgctatgatg
     6121 atttatacag tatggatatg tttggggaaa gcatagaggg acataagtat aagacacctt
     6181 atcaagtcgt gaaaatgata aacccgaact caaaagaaga tatggaactg ttacgagaag
     6241 tattaaggaa taacaatttt gatgatgagg acgagtaact tttttcctct tgcgagaaga
     6301 tacagcaaac aaaaagcccg atcaagagtg caaagcacca ccaatggtgg caaagctctt
     6361 gacagggctt tttcttttct gttgtgggca atcaagagga agaaagggat aaaatctgat
     6421 ttgtggtaaa tcaggcaaag gcaacgcagg tatctcaatt tgatttggta cttgaaaaga
     6481 acgggagtaa ttggaagatt atagaataac aaatattggt acattattac agctattttg
     6541 taatcacgta ctctctttga taaaaaattg gagattcctt tacaaatatg ctcttacgtg
     6601 ctattattta agtatctatt taaaaggagt taataaatat gcggcaaggt attcttaaat
     6661 aaactgtcaa tttgatagtg ggaacaaata attggatgtc cttttttagg agggcttagt
     6721 tttttgtacc cagtttaaga atacctttat catgtgattc taaagtatcc ggagaatatc
     6781 tgtatgcttt gtatgcctat ggttatgcat aaaaatccca gtgataaaag tatttatcac
     6841 tgggattttt atgccctttt gggtttttga atggaggaaa atcacatgaa aattattaat
     6901 attggagttt tagctcatgt tgatgcagga aaaactacct taacagaaag cttattatat
     6961 aacagtggag cgattacaga attaggaagc gtggacaaag gtacaacgag gacggataat
     7021 acgcttttag aacgtcagag aggaattaca attcagacag gaataacctc ttttcagtgg
     7081 gaaaatacga aggtgaacat catagacacg ccaggacata tggatttctt agcagaagta
     7141 tatcgttcat tatcagtttt agatggggca attctactga tttctgcaaa agatggcgta
     7201 caagcacaaa ctcgtatatt atttcatgca cttaggaaaa tggggattcc cacaatcttt
     7261 tttatcaata agattgacca aaatggaatt gatttatcaa cggtttatca ggatattaaa
     7321 gagaaacttt ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt
     7381 gtgacgaact ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa cgatgacctt
     7441 ttagagaaat atatgtccgg taaatcatta gaagcattgg aactcgaaca agaggaaagc
     7501 ataagatttc agaattgttc tctgttccct ctttatcatg gaagtgcaaa aagtaatata
     7561 gggattgata accttataga agttattact aataaatttt attcatcaac acatcgaggt
     7621 ccgtctgaac tttgcggaaa tgttttcaaa attgaatata caaaaaaaag acaacgtctt
     7681 gcatatatac gcctttatag tggagtacta catttacgag attcggttag agtatcagaa
     7741 aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt
     7801 gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt
     7861 gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta
     7921 ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt
     7981 ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa
     8041 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
     8101 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
     8161 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
     8221 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
     8281 cttggatact taaatcaatc gtttcaaaat gcagttatgg aggggatacg ctatggctgt
     8341 gaacaaggat tgtatggttg gaatgtgacg gactgtaaaa tctgttttaa gtatggctta
     8401 tactatagcc ctgttagtac cccagcagat tttcggatgc ttgctcctat tgtattggaa
     8461 caagtcttaa aaaaagctgg aacagaattg ttagagccat atcttagttt taaaatttat
     8521 gcgccacagg aatatctttc acgagcatac aacgatgctc ctaaatattg tgcgaacatc
     8581 gtagacactc aattgaaaaa taatgaggtc attcttagtg gagaaatccc tgctcggtgt
     8641 attcaagaat atcgtagtga tttaactttc tttacaaatg gacgtagtgt ttgtttaaca
     8701 gagttaaaag ggtaccatgt tactaccggt gaacctgttt gccagccccg tcgtccaaat
     8761 agtcggatag ataaagtacg atatatgttc aataaaataa cttagtgtat tttatgttgt
     8821 tatataaata tggtttcttg ttaaataaga tgaaatattt tttaataaag atttgaatta
     8881 aagtgtaaag gaggagatag ttattataaa ctacaagtgg atattgtgtc ctgtatgtgg
     8941 aaataaaaca cgattaaaga taagggaaga tactgaatta aaaaaattcc ccctctattg
     9001 tccgaaatgc agacaagaaa atttaattga aataaagcag ttcaaagtaa ctgtgattac
     9061 agagccagac gcaaagacgc agagccgata aaatgagatt aatacaatct cattttatcg
     9121 gctctttccg ttatgtatgg attcttttaa ttagtcttcg atgtttcttg cttcgttgat
     9181 accgctggct aaagattcca ttaaggatag ttctttgtct gtaaagctat ccatgtattt
     9241 ctctatctgt aatcgtcggg tgctttttac caagttatta gcaggtaaga aaaattcatc
     9301 aacggaaaca tgaagtaacg atacaaggtc ataaagaact tgtatgctgg ggtgttgccc
     9361 tttattttca atattagtta agtaccgtgg gtcaatttca atcaatgctc ccacttgttc
     9421 acgagttaaa cctcgtttca atcgagcttc tttaatggct aaaccaaagg ctctaaaatc
     9481 atatttatct tcttttttac gcatagtaga ccacctctat acattttatt gttcctactg
     9541 aattaaaaac aggtatagaa aaacgtgtta tatggtttat aggtttatat ttaataaaaa
     9601 gcactactaa acgccaataa aaaaaaccgt tatatggtag tgctatttac gctgttaaaa
     9661 tattgtatat tacttccaaa tggcggtttg ttggaggtca acgtcgccat gaagtacatc
     9721 atatacaata aatttcctta cattgggttc ttgtcaaaaa aagtcgtcta tctgcaatag
     9781 ataagtacgt ccaccaatgt ggttttataa atcatataga tagaataaca gaagcatgta
     9841 aacagagaaa taaatctgtt tatatgcttt tttggctatt cagaactttt ttacaaagtt
     9901 tatttatcag taatgcaaca aatccccctt tcacattggg actaagagtg aaaggagata
     9961 aacgagcaag gctcacttcc tttcctagac agaaaggggg tgagattatg aggacatctt
    10021 ctttcgagca tatcgtcaga ttacagttca attctttaat gctgattgtc attaagaaca
    10081 aagtgaaaag tcgaaacaga cagcttgcaa gacattctaa acgtgaagta ttgttttgtg
    10141 aaatagccga aacgaaacaa gttgaacgtg gaacgattga tacctgtttt tatgattgtg
    10201 tatcatttaa agtcttgcac tttacaattt atgtatctga tgaaacactt ggtacagttt
    10261 tacatagact atcagaaaaa cagcgtagtg cgattttatt acgttatttt caaggaatga
    10321 acgacagaga aatttcagaa ctgtaccatg tatcacgttc tgctatttgc agtagaagaa
    10381 ctagaggact aaggaagtta aaaatgcttt tgagcgaaag gaagtaaaga ttatggctat
    10441 ccctcttttg aattgatttg caaagcgtca agcggagatg aaatggcagt taaagagatt
    10501 ttgaagtttt atgacggtta tatttctaaa ttatgcttgc gtccttttta ccactctgaa
    10561 agtggtaaaa tcattatgca ggttgatgaa gaactaaaag gagaaattca tacagacatg
    10621 atgaaagcaa tattgaaatt tgaaatcgga gtgaagtaat caactgtata aaaaaatcgg
    10681 gagatacaca gtttcgtgtt atctccctta gtagttttac ggtcatatca aggctcattc
    10741 aatcgtggta aattcgtggt aagatgagtg tttttctata cttcaaaatg cttgaatgta
    10801 tagtgtttat gcggataatt gaaaattaga tataaaattt aataaaaacc aaaaatgcac
    10861 ggtcgccatg aagggaccgt gtatttttat cttcttattt tctcacctaa tcgtatcagc
    10921 cgcatcgctt gttttcctgc acaaacaagc aatcgctcta cctgttccat cgcataagaa
    10981 agtaccatcg gttcattgat gatatcaagt acatagtcga taccaccctc acttaattga
    11041 gcttgatttt tcttctccat attatgtagt tgtgggacaa ctttctgaca atcagcaatt
    11101 gtgtcaagaa cggatcaatc ctttattgga gttgctcgaa agggtcttct cttattacgg
    11161 acaggctttg ctcactgtac atagacaaca aatcattatc ctagtcaatc ggatttccag
    11221 agcctcattg ctatcattgc ttgataaaat ccagacaaag tttaataaaa tgtatcagtt
    11281 acagctaaac tttggaattg ggtcgctttg ttatacagag caagagacac cgcaatcttt
    11341 ccttcatgcc aaacaagttt gcgaatggat agctttccat caatctgtga acgagatccg
    11401 cttttttgaa gatttagacc tcgggatcgt tttaccagca atcccgtcag atcaacgaac
    11461 attatatgta aaacgtattt tgaaaagcct gacagaagaa gaagtacatt tgttcaagaa
    11521 gactttggca tgcttcagta aaaataacgg aagcatcaaa aattgctcag aagaattatt
    11581 tattcataaa aacaccttgc aatatcgatt gaacaagttc cattctctca ctggttatac
    11641 ccctagaaac tatgacgatt atcatattct aaagctggct tttttgttgg tacaaacatg
    11701 acaactacaa aaaaaccagg cgaacatatg attgttcgtc tggtttttaa ttattaatga
    11761 cttagcgttt ttactagaaa aactacttcc gctttttccc gccaaaagca tctttaaact
    11821 tatcgaagaa accgtcttct tgctgttctt caactttgat gccgccagct tcagcgaatg
    11881 cacgtaatgc ttctctttgt tgttcattta gatttttcgg tgtgatcagt tttactttga
    11941 catgttggtc gcctgttgca ccaccacgta acttcggagc gccttttcca cgtaaacgga
    12001 agttcgtacc tgtctgtgtt ccagcaggga tcttcaattt cacatctcca tggacagttg
    12061 gtacttggac ttcatccccc aaagctgctt gtacaaaact taaaggcaat tcatagtaaa
    12121 tttcagaacc ttcacgatca aagatatcac tttcttctac acggaagacc acatacaaat
    12181 caccaaatgg tccgccgttt tctccagctt ctccttgacc agctaaacgc atttgttgtc
    12241 cttcttctac accagctggt acattcactt taactgaatg tgctttcttc tcatgtcctg
    12301 agccatgaca ggttggacaa ggttctttga tttctttccc tgtcccatga caaacatcac
    12361 aagtttgacg gctcatcact cgtccaagtg gtgtttggcg ttctacatta atcgtacctg
    12421 caccatgaca tttatgacaa gtagttggtt gtgtgcctgg ttttgcaccg ttcccaccac
    12481 aagtatgaca gatttcttcc cgattatact tgatttcttt ttctacacca aaaatcgctt
    12541 cttcaaaaga caagtcgacc gtatattgca aatcagcacc ttgacgaggc gcatttggat
    12601 cgactgaccg accgcctcct ccaaagaaag agtcgaagat atcttcaaaa ccaccgaagc
    12661 cgccaccaga gaagccgccg aatccaccaa atccgccacc agctccacca gcaccgtaat
    12721 ttggatctgt gcctgcatgc ccgtattggt catatgcagc gcgtttttgc gggtcactta
    12781 aaatctcata tgcttctgat acttctttga atttttcttc cgcatcaggt tctttattga
    12841 tgtcaggatg gtattttttc gaaagcttac gataagcttt ctttatttca tcttctgatg
    12901 ctccttttga gagccctaag acttcataat aatcacgttt tgttgccata ggctttcctc
    12961 ctattggttc tcatcatttt cacgtcgctt gttatcatat catattttgt ttgaaacaaa
    13021 aaccgatact tcaaactatt tttcaaaaga aaaagccaaa gtagcaactt tggccttctc
    13081 tcgaaattca gtgaactgat tatttatcgt cgccatcgac ttcttcaaaa tcagcatcaa
    13141 cgacatcatc tgctccgcct tgagctgctt ctgggttttc ttgagcttgt tgttgtgccg
    13201 cttgttcgta tagtttaaca gtcaagtttt gaacgatttc gttcaatgca tcgcgtttag
    13261 ttttcatttc ttcgatgttg tttgcttcaa cagcggcttt caattcatcg cgagctgttt
    13321 ctgctttttt cacttcttct tcgtccactt tgccttctaa ttctttcaat gttttgtcca
    13381 cagagaatag caatgcatct acatcattac gtaggtcaac ttcctcttta cgagctttat
    13441 ctgcttctgc atttgcttct gcatctttta ccatacgttc gatttcttcg tctgttagac
    13501 ctgaagaaga tttgatcgtg attttttgtt ctttttgtgt acctagatct ttcgcagata
    13561 catttacaat cccgttttta tcaatatcga atgttacttc gatttgtgga ataccacgag
    13621 gagctgctgg aatatctgtc aattggaatc ttcctagagt tttgttgtca gcagccattg
    13681 ggcgttcacc ttgtaataca tggatgtcta ctgctggttg attatctgcc gcagttgaga
    13741 atacttgtga tttacttgtt ggaatcgttg tattgcggtc aatcaattta gtgaacactc
    13801 cacccattgt ttcgatacct aatgacaatg gtgtaacgtc aagtaaaaca acatctttca
    13861 catcaccagt gatcacgcca ccttggatag cagcacccat agcaactact tcatcagggt
    13921 taactgattt gtttggttct tttcctgttt ctttacgtac tgcttctaca actgcaggga
    13981 tacgagtaga tccaccgact aaaataactt cgtcgatttc tgattgtgat aaacctgcat
    14041 cttttagcgc ttgacgtact ggtacttttg tacgttctac taagtcagct gttaattcat
    14101 caaattttgc acgtgtcaat gtcatttcca aatgcaatgg tccagattct cctgctgtga
    14161 taaatggcaa gctgatttgt gtgcttgata cgccagataa atcttttttc gctttttcag
    14221 cagcgtcttt caagcgttgt aaagccattt tgtcttgaga caagtcaatg ctgttttctt
    14281 ttttgaattc tgctaccatg tagtcgatga ttttgttatc aaagtcgtcc ccacctagat
    14341 ggttgtcgcc agctgttgac aatacatcaa agacaccgtc tcctagttca aggatagata
    14401 cgtcaaaagt accgccacca aggtcgaata ctaagatttt ttcgtcgcga tctgttttat
    14461 ctaaaccata ggcaagagca gctgcagttg gttcattgac gatacgttct acttctagac
    14521 cagcgatctt accagcatct tttgttgctt gacgttgtgc atcattaaag taagctggca
    14581 cagtgataac tgctttttcg actttttcac ctaaatagtc ttctgcgaat cctttgatat
    14641 attgaaggat cattgctgag atttcttgtg gtgtatatga tttaccttct acatctactt
    14701 tgtaacctgc ttcaccgata tggcgtttga ttgatgaaat cgtatttggg tttgtcacag
    14761 cttgacgttt tgcaacttcc cctacttgga tttctccatt tttgaatgat acaacagaag
    14821 gtgttgtacg gtttccttct gggttagcga tgatttttgc ttcgtttcct tccaatactg
    14881 ctactgcaga gttagtcgta cctaagtcaa taccgataat tttactcata attaacgtct
    14941 ccttctttaa ttgaaagtgt aagttttata tccgcacaat tattgtgcaa caatgaccat
    15001 ggttggtctt aacacgcgat catgtaattt gtagccttct tgtagtactt ctacaatcgt
    15061 atcggctggt gtatcttcag tcgctggaac agtttgtact gcttgatgaa gattaggatc
    15121 aaaggcttct cctttagcag ggattttttc aattccttct tcttccagtg cgttacgcaa
    15181 actttccaag accatttcca cgcctttttt caaacctgcc ccttgatcat ctgacacttc
    15241 tgtggctaat gcacgttcca ggttgtcaat tgatggaagt aattttttgg ctaaatcttg
    15301 tgaacggtat ttttgtaatt gctcacgctc attttttcct cgatttgcca tatttgcgat
    15361 ttcagcacga gcccgcaagt atttatcttc catttcttct aactcagctt ttaatttcgc
    15421 tgtttcgaat tcttccgctt cagcttctga aatacccgaa tcttctgcag cttcgacatc
    15481 gatttccggt tctggctgag catctgtcat ttctttatct agttcttctt gattttcttt
    15541 cacgatacag tcttcctttc attattcagt cagctgtttg attgctctta ctaagaaaga
    15601 gagaacgatc agctgcaaat agcttcgtta ttcacattac gagtttgaca agtcaagcga
    15661 acggtagtaa tcagcaagtt tagctgctag ttctcttcga taaacatcga ctagaccgaa
    15721 catccgagaa taaggcatac tagtaggccc taacaaggct attgtacctc ttccatgacc
    15781 atctatctcg taacttgctt gaatcatact catattctgc aataaatcat tgcctaattc
    15841 agcaccaatc ttgatatgaa tattgctatc catcggagtg atcatttgtg taagtccgtc
    15901 tgagtctttc ataaaagaaa acatcgattt gaactgatca agatcttgac ggggctcata
    15961 atccaagaga ttcatttgtc cactgacata aaccttatct tcaaaaacat gaccaagaac
    16021 ggtgtcaaat aactgagaca tcccttctgt cgtttggaaa tatttatgaa gaatcatcgg
    16081 aatctccgta cgaagtttat ggtacaccgt catcagagga tctccgacta gacggtcgtt
    16141 tacaatctgt accatttttt ctagttcctg acttcccata tattcgggaa gagtgaacac
    16201 ttggctttct acattcccca tatctgttac gacgatagca ataatctgtc gattattcaa
    16261 gggtacgata cgaaagccag tcagtcggcg gtcttttatt tcaggtccca atgaaaaagt
    16321 cgtatagctt gtcagctcag ataatatctc agcagattgc ttaataatct cattgatttc
    16381 atgaaattct ctgcctagag actgacgaat cagcagcaaa tcatcttctg ctacgcgagc
    16441 aggttttaac aaatgatcca cgtaataacg atagcctaaa gcagatggaa tccgcccaga
    16501 agaagagtga gtcttcaaca gcaatccttc atcttctaat gctttcatat cattgcgaat
    16561 cgttgctgag ctggccttga ctccctcaga catcaatgtt ttggaaccaa cgggcacgcc
    16621 actactcgta tagtgttgga tgatcagacg taaaatgtcg ttttgtcgct ttgttaacat
    16681 cttcctcacc tctcattagc actcatatat tctaagtgct aatccactta taaatatagc
    16741 aagaagccct tctcctgtca agagaaagac tccatttttt agcactctaa acacaagagt
    16801 gctaattcta aataattgat gtaaatccag ttatatcaac gtttcaatag tcctttattg
    16861 tatttttgaa ctttttttat ttaaattcag ataaaaaaca aatattcttt aaactatttt
    16921 tcaaacaaaa agcgctcaaa cacattgttc cccaaaaagg tccctcttgg tgtcaaataa
    16981 acacggtctt cttcattaac aagtaaccct tcttgctcta attcccttag cacatcaccg
    17041 tacaccgatt caattgtttt ttgatagcga tcatagaaat gttttttagt aatccctgtt
    17101 tttttcttag tcctaagaac atttcttctt ctatttgatt ttttcgtgtc aatttttctt
    17161 cttccaatac cgggcgttca tttgctcgta atgggcgtaa atagtgttgg attggtcctt
    17221 tgttgcggta acgaatatct ccaagataac cgctagcacc agctccaaac ccaaaatagt
    17281 ggtcattgtt ccagtacatg aggttatgtt gagattcatg tccggagagc ccaaagttac
    17341 ttatttcata ttggtgacgt ccatgctttt ccattgcttc aatcgtctct tcaaacattc
    17401 tggtttccgt ttcctgatca ggcagttcga gtcttccttg tcgtacccag ttcatgaaca
    17461 ttgttttgtt ttcgaggatc aatgagtaca aagaatagtg aggcagatcc aaagccaaag
    17521 ctttttccaa agtatccaga tatccttcta gtgtttgtcc cggtaatgca taaatcaaat
    17581 cgatgcttac attggtaaaa ttttctgctt ctaaaaattg catagtttga tacacatctt
    17641 ctgcggtgtg tttacgtcca atttttttca ataagcgatt atcaaatgtc tgcactccca
    17701 tggataaacg attgacgcca tagtttttca ttacttgaag tttttcacgg gtcaaatcac
    17761 ctggattcgc ttctacagta aactcattac cattttgaaa aggcaggatc tctctagcac
    17821 ctgataacag tcgatcaagt tgtttagcag ataaagaagt tggtgtcccg ccccctacgt
    17881 aaagtgtttc taaattcgct gtcggatgac tttctagcat tattttcatt tcattcagca
    17941 gcatttccac atactcatct actggctgcc cttctaaaaa aactttatta aaatcacagt
    18001 aatagcagat atgctcgcaa aatgggatat gaatgtacgc agaaaccttg ttttctgcgg
    18061 gatttattgt ttctatcatt gaaattcttt ctccttacgt cgaaaagtcg tgcctgtttt
    18121 cgaccttctt aaaatcgcgt tcaagcacca actcattgaa ataagccgca tatttagaat
    18181 caaggaaacc tagctctaaa aatgcttgct tattgtcatc ggagctacac ggtgctttta
    18241 cagcctctcg ataaacggta atcgcaaagc tgaccttcgg cattaagtca aaatctgcaa
    18301 aaatatgaaa agctattttc acagattcct ccttaagtgc tcagaccaca acgctttgct
    18361 tacaacctct catatacgca tactaaacgt atttggcgaa caaatccact aaaaaatcca
    18421 agaatcgact tcttggatga ttttttcttt cattaaactg gttacctagt tatatatctc
    18481 tagggttgtt ttcatgtctg agtgcccgat atgatttatt tttcgtttct ataattactt
    18541 tttgccttcc agtttctttt aatattgtac tcaagcaagt gggaattggt tcttttgtca
    18601 tccatgtatg caaaatgtta taatgctggt aaacaaaaat gattacctgt catttttatt
    18661 tagttcgtat ttaattaagt tgactatctg tatactctgt ctaaaactta agaattgttg
    18721 ttttcgctgt cgctacccaa ttttaatgaa tgtctgtagg ttaaaactgt tgtccttgtt
    18781 atagatagta aaggagagct tgaaaatgaa aactgcgatt acgtttctat tagaggaata
    18841 tgccgattgg gaaggtgctt atattactag cacactaaac caaaatgaga aatggcaaaa
    18901 caaaactgct tctatccaat cagaggtcac ttccattgga ggattaaaaa caaaaatcga
    18961 ttacttaatc gaacaaatac ctgaaaaagt tgaccttttt attatgatcg gtggcaattc
    19021 atgggacata aaaaatcatc atttatatca aataatcgat aaattgttaa aaacagacgt
    19081 accagtagct gctatttgtg gggcagttga ttatttagct cagagcggat tattgcaagg
    19141 ttacaaacac accggaaatg cgcaatttcc ttggttagaa tcggaaaatt atcagaataa
    19201 agaagatttt atagaacaac aagctgtgaa agatcgtaat ttgattactg cgaatggaac
    19261 agctcctata gaattcacag acttgattct tcaagcagtc cattttgaaa cagaagaaat
    19321 aagagaaaaa acgattcgtc tatacaaact tggattctat gattactata aaaagtacgg
    19381 agatcctttc tcctaatcat tctcttcaat taaatactcc ttatcaatag tgtgacaagt
    19441 aattttactg atgattattt gtcacacttt ttctgacatt tatcaaagaa gatacactct
    19501 atttatattc atagacaagg ttttacggat tcaaaaattc aggattatgc tatactggta
    19561 ttaaaaaaat aatgaggtat tttatatgaa aaagttaggt tttattttat tgttggtctc
    19621 tttattcctt gtaggatgta cgtctcctag taaagaaaat agtgatccat ctgcgaccac
    19681 agacaaaagt tcaatcagac aaaccagttc agctgaaaaa tcaagtactc agaaatcagc
    19741 ttctactgaa acgaatacat cttcaacaag cagtaaagtc tcagagccca atacttcttc
    19801 tacatctagt agtacagttg agacaaatat ttcttcttca acaatagaca caacctcctc
    19861 acagtcttct cagtcaatag atccgttagc aggatattct gatctacaaa ttgagtacgc
    19921 acgtgtttgg ctagctatta gaggtaatac ttttagagat acatcgggtg atttcgaatt
    19981 acatgttatt cattatcctg caggaacacc tatcgcccct tatgatgcag ggagtgcagt
    20041 ctatccgaaa gatgtggtta tgctcactgg gaaatacagt taccagggag ttttagtgta
    20101 ttcaagtaac catgacggga caatcactag ttatccagtt ccttcacatt ggcaaatgcc
    20161 tgcagatcaa acatcttatc cagcgttcat tagagaaaca actcaagaaa tcattgataa
    20221 tgcatctgta gtagctattc ctacaggaaa tcccaatgac gtgaaacagt taatcgatgt
    20281 gacagttata gataattaga ctttctaata tgttatacaa aaagaagctg acagc
//
LOCUS       DAZKOK010000048        20284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000048 DAZKOK010000000
VERSION     DAZKOK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.048"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..1646)
                     /locus_tag="ACG90W_001476"
     CDS             complement(87..1646)
                     /locus_tag="ACG90W_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0477843.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1801..4035
                     /gene="pcrA"
                     /locus_tag="ACG90W_001477"
     CDS             1801..4035
                     /gene="pcrA"
                     /locus_tag="ACG90W_001477"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0477844.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4052..6088
                     /gene="ligA"
                     /locus_tag="ACG90W_001478"
     CDS             4052..6088
                     /gene="ligA"
                     /locus_tag="ACG90W_001478"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0477845.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6194..6499
                     /gene="gatC"
                     /locus_tag="ACG90W_001479"
     CDS             6194..6499
                     /gene="gatC"
                     /locus_tag="ACG90W_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0477846.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6499..7971
                     /gene="gatA"
                     /locus_tag="ACG90W_001480"
     CDS             6499..7971
                     /gene="gatA"
                     /locus_tag="ACG90W_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0477847.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7968..9398
                     /gene="gatB"
                     /locus_tag="ACG90W_001481"
     CDS             7968..9398
                     /gene="gatB"
                     /locus_tag="ACG90W_001481"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0477848.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9408..10463
                     /locus_tag="ACG90W_001482"
     CDS             9408..10463
                     /locus_tag="ACG90W_001482"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0477849.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10574..11947
                     /gene="rlmD"
                     /locus_tag="ACG90W_001483"
     CDS             10574..11947
                     /gene="rlmD"
                     /locus_tag="ACG90W_001483"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0477850.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11950..12078
                     /locus_tag="ACG90W_001484"
     CDS             11950..12078
                     /locus_tag="ACG90W_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0477851.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12518..12706
                     /locus_tag="ACG90W_001485"
     CDS             12518..12706
                     /locus_tag="ACG90W_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0477852.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12784..13797
                     /locus_tag="ACG90W_001486"
     CDS             12784..13797
                     /locus_tag="ACG90W_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0477853.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13864..14898)
                     /locus_tag="ACG90W_001487"
     CDS             complement(13864..14898)
                     /locus_tag="ACG90W_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0477854.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15013..17238
                     /locus_tag="ACG90W_001488"
     CDS             15013..17238
                     /locus_tag="ACG90W_001488"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0477855.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17256..19139
                     /locus_tag="ACG90W_001489"
     CDS             17256..19139
                     /locus_tag="ACG90W_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0477856.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19120..20094
                     /locus_tag="ACG90W_001490"
     CDS             19120..20094
                     /locus_tag="ACG90W_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0477857.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6838 a         3720 c         4344 g         5382 t
ORIGIN      
        1 ttcccagcct cttctttttt tatagaatca tatagaatca agtagtgtag atgaatagtt
       61 gttgtaaaat agtttttcca tgttcgttaa aatgattgct tcaaatttgt gatagaagat
      121 ttagcctcga tttcctgctt gattcttggc aagtttgctt catcagtcaa gataaccaaa
      181 atatctccta cattcatgat cgtatcaccg tgagtcaaga tttcctgatt tcctctgcgt
      241 atagagatta gcagcatatt tttaggccaa ttgaagtctc gtaccattaa tccgtctaaa
      301 gaactctctg ccgttactgg aaaatcaaaa gttgtccttg caccttttag tgaggcggtc
      361 tgagtatttc caagtagacg ttccaataat gattcgtaaa ttggattacc gccgagaagg
      421 tcattgacga tataagcaac aagagcagcc aatcctaaag gcatcagatg attaagagag
      481 ccaaccattt ctgtcactaa aataattgct gtcagcggtg cttttccaat cgctgtgaaa
      541 taaccagcca tggcaataac gacaaaatcc ttgatgtaga ttggatccat cccgaaatca
      601 accataatgt ttccataaaa gctaccgatt aaagccccca atgtcaatat cggaaggaaa
      661 atcccccctg gaagaccccc accgtaagaa atcatggaaa aaacgaatcg aagaacaaac
      721 aaaccgataa tcataatgat tggcaaggaa gcatgtccaa aagctaaaac gatttgattg
      781 cctccgccta gagtactagg ccataaataa ccaattggca aaatcagcaa gaaaggaacg
      841 aacccgtgaa aataagctgg taaaggcaat cggttgtaaa attttggcaa agccagcaag
      901 acattctgat aaataaaacc taagatgcct agaagtactc ctaagatcac taaggaacca
      961 taatatttca acggcaaaga aggaaggtca cctagatata aaacaggtct caagccgaaa
     1021 aagtaaagag agacgaagtt agcagtgatt gctgaagcaa atgatgtcag ccaaacaagt
     1081 ggagaaaagg agtgatggat ttcttctaaa acaaagagta atcctgcgat aggagcattg
     1141 aatgctgctg caagtccgct acttgcaccg ctagaaatta aaatcttttc ttctgaagat
     1201 ggactccgaa acaattgtcc agctccttgt ccaatggatg cgccaagctg tatagaaggt
     1261 ccttctctac ctagaaaaag gcccgcacca actgaaagta caccgccaat gaactttttc
     1321 cataaaacag agaaccagtt cattgataat tccccgcgaa gctgtcccac tacttgtgga
     1381 atcccgcttc ccttgatatc cggttcattt ttgaccagat aacccacgaa aaatgctgca
     1441 aggatgcagc aaatagctag gccaattaag taaatcggct gttcccgact catttcatag
     1501 atacttcgaa ctaaatcgct tagcttttcg atagataatc gaaacaggct aacgacgatt
     1561 cctgtcagca gtccaacaaa tattcctttt agaataaaat atatctgagt cttatctaat
     1621 ttcttgattt ttcctttttt ttccattcaa acgcctcgct ttcttttaat ttagcgcgaa
     1681 atcaacgaat tgacaaggaa aaatccataa aaagaaacaa atgattttga actagctagt
     1741 ttaatgaaag ccaagaaaat gctataatta acaagattga taaaagaaag gatgttgtta
     1801 atgtcatcca aagctgaatt attaaacggg atgaaccccc gtcagaaaga agctgttctt
     1861 cacactgacg gaccattact gctaatggca ggtgcaggaa gtggaaagac gagagtgctg
     1921 acacaccgca ttgcctattt gattgaggaa aaagaagtca atccttggaa catcctagcg
     1981 attactttta ccaataaggc tgcaaaagaa atgaaagaac gtgtcaatgc gattctcgca
     2041 tctggaggcg aagatgtctg ggtatcgact tttcactcga tgtgtgtgcg tattttaaga
     2101 agagatgttg attttatcgg gtataaccgg aactttacaa tcatcgattc ttctgaacag
     2161 ctgaccttga tgaaacggat actaaaagaa ctgaacattg atccgaaaaa atatgatcct
     2221 cgaagtatat taggaacaat ctctcaagca aaaaacagcc tgcagacccc tcaggatttt
     2281 gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa aatgttatgc tgcttaccaa
     2341 aaagagcttc aatataatca atgtatggac tttgacgacc tgatcatgaa tacgattcga
     2401 ttatttgaag aacatcctga ttcactcacg tattatcaaa acaaattcca ttatattcat
     2461 gtagatgagt accaagatac aaatcacgca cagtatacgc tagtcaattt attggcaggt
     2521 agatttagga acctgtgcgt cgtgggagat gcagatcaaa gtatttacgg ctggcgcggt
     2581 gcagatatgc agaacatctt ggattttgaa aaagattatc cagatgctgc agtaattttg
     2641 ctagaacaaa actatcgctc cacgaaaaac atccttagcg cagcaaacca agtcattgaa
     2701 aacaatagca atcgaaaacc aaaaaacttg tggacagaaa acaaagaagg aaacaagatc
     2761 acttactacc gtgcagataa tgaacgagac gaaacccgtt ttatcgtaga ccggatgcaa
     2821 gaagagattc gaagcaatca tcggaattac ggcgactttg ccatcttgta ccggacgaac
     2881 gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa atattcctta taaaatggtt
     2941 ggagggcaca agttctacga tcgtaaagaa atcaaagata ttttagcgta tttgaatgtg
     3001 ctggtaaatc ctcaagattc aatcagtttt gagcgtatcg ttaattcgcc caaacgaggc
     3061 atcggaccag gatctatcga aaaactacgc agttttgctt ctcttcatga atggccactg
     3121 cttgaagcag cgcaaaatgt ggatctggca aatatcggtg gaaaagcagg acagcaatta
     3181 ggtgcatttg gagagatgat ccaagaagtc actcagatga tcccatactt gacagtgaca
     3241 gaattgacga aagaggtttt ggatcgtagc ggatatctag aagacttaaa aatccaaaat
     3301 acattagaag cacaagcacg catcgaaaac ttggaggaat ttttaacggt cacacaagaa
     3361 ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg atgcaccaga agaaaaacta
     3421 acggtcttct taaatgatct ggcacttgtt tccgacattg acaatttaga agaagatgct
     3481 tcccaagtga cattgatgac tttgcatgca gccaaaggat tagaatttcc agttgtattc
     3541 ctgattggtc tagaagaggg ggtttttcct ttatctcgag cattgatgga agaaagcgaa
     3601 ctggaggaag aacgccgtct cgcatatgtg gggatcacgc gagcagaaga agcgttgtat
     3661 ttaacaaatg ctttctccag aacattatat ggacggacgc aatataaccg accttctcgt
     3721 tttgtagaag aaatcgatca ggagttactg gaaatcgaag gcatgcgtcc tacgcctaaa
     3781 aaaacgcctg tattcgctaa aaaaacagca tatagctata aacagccaga gacagcggtt
     3841 gttccctcta aaagtgctac tggaggggaa aataacagct ggaaacctgg agataaagtc
     3901 aaacacaaaa aatggggtct aggaacagtc gtccgagtaa gcgggacgtc gaaggatcta
     3961 gaactagatg tggctttccc aagtcaagga gtaaaacgtc ttttggctgc ctttgcgcca
     4021 attgaaaaag cttagtagtg gagggtaaat gatggaacat gaaatgacga ttgacgaagc
     4081 tgctaaacga gcagaagaac ttcgtacaag actgaaccaa tggtctcgcg aatactacgt
     4141 agaagataaa ccaacagtgg aagactatgt ttatgataaa gaatacgcag aattagtagc
     4201 aatcgaggaa caatatcctg atttgatcac gtctgattct cccactcaac gagtaggcgg
     4261 aaaagtatta gaaggttttg aaaaagtcac tcatgatatt ccgctttaca gtttgaacga
     4321 cgttttcagc aaagaagagc tgatagcttt tgatcaacgt gtacaaaaag cagtgggacg
     4381 tgtagttgac tattgctgtg aactaaaaat agatggactt tccgtttctt tgcgttatga
     4441 agatggaaat ttcgtgagag gtgcgactcg cggggatgga actgtaggag aaaacatcac
     4501 tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta aaagaaccaa tgaacatcga
     4561 agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc caattgaacc aagacagaga
     4621 agcagaggga aaagatatct ttgcgaatcc tcggaacgcc gcagctggtt ctttgcgtca
     4681 gttagattct aaaatcacag cgaaaagaaa tctggacact tttttataca cagtagctga
     4741 tttcggtcct atgcaagcaa aaacccagta tgatgcgcta gaagagctag aaaaaattgg
     4801 attccacacg aatcgggaaa aacgtctatg tcattcgatt gatgaagtgt ggtcatacat
     4861 tgaagaatat catgacaaac gtgtggattt gccctatgaa atcgatggaa tcgtcatcaa
     4921 agtgaatgaa ttctcgctac aagatcaact tggttttact atcaaagcac cgagatgggc
     4981 agcagcttac aagtttccgc cagaagaagt tgaaacgcta atcgaaaaca tcgaatggac
     5041 agtcggtcga acaggggtag taacgccaac agctatcatg acaccagtcc gagtagcagg
     5101 aacgacagta agccgtgcca gcctgcataa cggtgattat attaagttga aggatatccg
     5161 cttgaaagat acagttctga tttataaagc gggagacatc atcccagaag tttcgcaagt
     5221 cgtgttagat aaacgaccta aagacagcga agaataccaa ttgcctacac attgtccagt
     5281 ttgtggaagt gaacttgtcc atttggatga agaagtagcc ttacggtgca tcaatcctaa
     5341 atgccctgct caaatgaaag aaggtttgaa ccactttgtt tcaagaaatg cgatgaatat
     5401 cgatgggttg gggccgcgcg tactagaaca gatgtatgat aaaaaactag tagcagatgt
     5461 tgctgatctt tacaaattga cagaagaaga gttattgacg ttggacaaaa taaaagaaaa
     5521 atcagcaaat aatattttga cagcaatcga caacagcaaa gataattcgg tcgagcggtt
     5581 gattttcggg ttaggaatcc gacatgtcgg agcgaaagca gcaaaaattt tagcagaaca
     5641 tttcggtgat ctagaaacat tgagtaaaag tgactatgag tcgattattg cgcttgatac
     5701 gatcggtgat atcattgccg acagtgtagt cacttatttc agtaacgaag aagtccatga
     5761 attgatgaat gaattaaaac aagcaggcgt caatttcgaa tacaaagggc ttcgaaatgc
     5821 ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca gtagtcctta ctggtaaact
     5881 aacacgattt actcgtgaag aagcaaaaga gacgatcgaa aatcttggcg ggaaagtgac
     5941 tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga gaagatgctg gcagcaagct
     6001 gaccaaagca caagaattag gaatcgaagt ttggacagaa gatcaaatgg cagatgcatt
     6061 ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt ttcagacaaa tttcaataaa
     6121 caatttcaga cacggcactc ttgtgttaaa atagagagca aagtttttaa ctcgtttaga
     6181 aagaaggggt agtatggcga ttagtgaaga acaagtgaaa cacgttgcca aattagcaaa
     6241 acttgcgttc gcagatgaag aacttgcaag ttttaccgac caattaggta aaatcatcga
     6301 tatggttgaa atgctggaag aagtagatac ggaaggtgtt ccatttacgt cgaatgtggt
     6361 tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct ggatggaacc gcgatgaatt
     6421 attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa gtacccgcaa tcatcgataa
     6481 tggggaggct ggcgcataat gacagaatta cacaatcgtt ctctagaaga actgcatcat
     6541 atgcttgtat cgaaagaaat cactgtacaa gatttgacaa aaacaacatt tgaacgaatc
     6601 aaagaaacag agcctgaggt cgaggcattc attacattga acgaagaaaa agcattggaa
     6661 caggcaaaag ctttagatga aaaagggatt gatgaaagca acgtccttgc tggtattcca
     6721 attggtatca aggataatat cgtaacaaaa gatatcttga caaccgctgc atctaagatc
     6781 ctttacaatt ttgaaccaat ttatgatgca acggtcatgg ataaaatcta tcaagcagat
     6841 atgatccctg ttggtaagct aaacatggat gaatttgcta tgggtggaag tacagaaact
     6901 tcttatttca agaaaacaaa aaatgcttgg gacccaacaa aagtacctgg tggctcttct
     6961 ggtggttctg cagcagcagt tgcagctgga caaattccag tttctttagg tagtgatact
     7021 gggggaagta tccgccaacc agcagctttt aacggcatcg tagggatgaa acctacttat
     7081 ggccgtgtct cacgttttgg tttgattgcc tttgcttcat ctcttgacca aatcggacca
     7141 atgacccgta cggtgaaaga taatgcatta gcattgacag ctatcagcgg ttatgacgaa
     7201 aaagacggta cttcttcagg cgtttcagtt cctgattttg cagagggact gacaggcgac
     7261 atcaaaggaa tgaagattgc tttacctaaa gaatacttag gcgaaggtgt ggcccctggc
     7321 gtaaaagaag ctgtattgaa agcagcagaa acattccgtt cattaggtgc tgttgtggaa
     7381 gaagtcagct tgcctcattc aaaatacggt gtggctgttt attatatcat tgcctcatca
     7441 gaagcaagtt ccaatttgca acgattcgat ggtatccgct acggttatcg ctctgaaaat
     7501 gtgaaatcat tagatgatgt atatgtcaac tcacgttcag aaggattcgg tgaagaagta
     7561 aaacgccgta tcatgttagg tacattctcc ttaagcgcgg gttattatga cgcttacttc
     7621 aaaaaagcag gacaagtccg tacattgatc aaacgagatt ttgaaaaagt ctttgcagat
     7681 tatgacttga tcatcgggcc atcttcacca acagttgcat tcggaatcgg tgagaacatt
     7741 aacgatccaa tcacaatgta catgagtgac atcttaacga tccctgtcaa cttggctgga
     7801 cttccaggta tgtctgtacc ggcaggttta tcagaaggat taccagttgg attgcagatc
     7861 atcggtaatt attttgatga aaaaacgatg tatcaagcgg catatgcgtt tgaacaagca
     7921 acagaattcc ataaacaaca accagctatt ttaggaggga aagacgaatg aattttgaaa
     7981 cagtcattgg actagaagtc cacgtcgagt taaaaaccaa ctcaaaaatt ttctctccag
     8041 cgccagctca ttttggtgca gaaccaaaca gcaatacaaa cgtgatcgac tggggttatc
     8101 caggcgtgct gccagtaatg aataaagctg cgctggaatt cgggatgaaa gcagctttgg
     8161 cattaaactg tgaaatctct aaagatactc attttgaccg gaaaaattac ttttacccag
     8221 ataatccaaa agcatatcaa atttcccaat ttgatcaacc aatcggtcat gacggatgga
     8281 tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat cgaacgtgtc catttagaag
     8341 aagatgctgg aaaaaacatg catggtattg gcggttattc ttacgttgat ttgaaccgac
     8401 aaggcacgcc tttgatcgaa atcgtttctg aagctgatat gcgttcacca gaagaagcat
     8461 acgcttatct agaagcattg cgatcaatta tccaatttac agaagtcagc gatgtgaaaa
     8521 tggaagaagg ctctatgcgc tgtgatgcca atatttctct tcgcccttat ggacaagaag
     8581 agttcggtac gaaagcagaa ttgaaaaact tgaactcttt atctttcgtg aaaaaaggtt
     8641 tagcttttga agaaaaacgt caagcaaaag ttcttctttc aggaggagaa atccaacaag
     8701 aaacacgccg ttttgacgaa acaacgaata aaacagtttt gatgcgtgtc aaagaagggt
     8761 ctagtgatta tcgttacttc ccagagcctg atatcccgaa attcgtgatc gatgacgaat
     8821 ggattcaaaa agtaaaagct agtttgcctg agatgccagc ttctcgtcgc gaacgctata
     8881 tccgtgagtt tggacttcca gagtatgacg caatggtatt gacacttaca aaagaaatgt
     8941 ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc caagcaagca tctaactggt
     9001 tgatgggtga agtttctgct tacttgaaca gtgagaaact ggaattagct gagacaaaat
     9061 tgacacctga gaacttagca ggaatgattc gtttgatcgc cgatggaacg atcagctcaa
     9121 aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg cggagaagcc caagaagtag
     9181 ttgaaaaaaa tggttgggtg caattatctg atccgaacaa actactgcca atcattaatg
     9241 aaattttgga taacaaccaa caatctgtcg atgatttcaa aaatggaaaa gatcgtgcgg
     9301 ttggcttcct agtcggtcaa atcatgaaag caactaaagg acaagcaaat cctggtgtcg
     9361 tgaataagct acttaaagat gaattagcga aacgatagag gctgaaaatg aaaaaagcac
     9421 gcgtaattta taaccccacc tcgggaaaag aattattgaa aaaaaatttg gcggatatcc
     9481 tacaggcatt agaagaagca ggatttgaag ccagcgccta tgcaactaca ccagaaccag
     9541 attcagccaa gaatgaagca tgtagagtcg cagaactagg ttttgatcta gtagtggcag
     9601 ctggtggcga tggcacaatc aacgaagtgg ttaatggaat cgctccgctt gaacaccgtc
     9661 ctaaaatggc catcatccca gctggtacaa cgaacgacta tgctcgagca ttaaaaattc
     9721 ctagagataa tattaaagca gcagcagaag tgataaaaaa acaccaaacc gtaaaaatgg
     9781 acatcggcct ttctgataac agttacttca tcaatatcgc tgcaggtggc tatttgacag
     9841 aattgactta tgaagttcca tcagaactaa agagtatttt tggttatctt gcttatcttg
     9901 cgaaaggagc agagatgctc cctcgagtaa agccaatcaa aatgcgcttg aagtatgatg
     9961 aaggggaata tatcggaaat gcttcgatgt ttttcctagg gctgacgaac tctgttggag
    10021 gattcgaaca gatcgcgcca gatgccaaat tggatgatgg gaagttttct ttaatcattg
    10081 tgaagacagc aaatatcttt gagatcttgc atattgcagc actcatgctg aatggcggtc
    10141 gacatgtcga tgatccaaga ataatctata ccaaaacaag cagtttgttc gttgaaacag
    10201 aagatgatat caatcgacca gtaatgatca acttagatgg tgaatacggc ggggaagcac
    10261 cgatgcattt ccaaaatctt catcaacaca ttgagttctt tgccaatacc gatcagattc
    10321 ctgatgaagc aatcacaggt acagatgaag aagaattaga ggttgtcagc aaagaattcg
    10381 taaaagaagt cgaacgttta acggatgaag atatcgacgg tgacggaaaa atagcagacg
    10441 aaacacctaa tcaaaagaat taatcaaaag ccggtgctga acgtagtaag gtcagtgccg
    10501 gttttagttg tgtaattagc tgatttatag tagagttcag taggattgaa aaataggctt
    10561 aaaggagcaa aatatgacag aacagcaaat agtaaagaaa aacgaacgat atacagtcga
    10621 aatcgtcgac ttgagttatg aaggaatggg ggtagcaaaa atcgatggtt atccattatt
    10681 tatcgaaaat gcactccctg gagaagaagt agaaatacta gtagtaaaag caggcaataa
    10741 gtttggatat ggaaaagtcg aaacatttat tacacaatca cctgatcgtc agcctgtaga
    10801 aaacaatgca ttacttcgca taggtatcgc acctttagcc catttgaagt atgaacaaca
    10861 gcttgtcttc aaacaaaaac aagtagagca tgtaatggcg aagattgcta agatgccaga
    10921 agttcctgta cttccaacgg tcggaatggc agaaccattc ggctaccgta ataaagcaca
    10981 gatccctgtt cgccgtatcg atggacagtt agccaccggt ttttacaaaa agaatagcca
    11041 tgatctagtc cctatcgaag atttttatat ccaagatcct gtcatcgatc aagcaatcaa
    11101 agtagtcaga gatatcttgc aacggttcca agtacgagga tataatgaag aaaaaaatga
    11161 aggacagctt cgtcacatca ttatccgtcg tggtcattat tcccatgaaa tgatggtcgt
    11221 tttagttact cgtaaagaaa aattctttaa atccaaggaa attgctgaag ctattcatga
    11281 agaattgcca gaagtggttt cggtcatcca aaatatcaat gaagaaaaaa caaatgtgat
    11341 tttaggagac aaagaaaaag ttttatatgg cagagactat atcgaagatc agctattagg
    11401 aaaaacctat cgaatctcag caaagtcatt ttatcaagta aatactcaac aagcagagta
    11461 tctgtaccaa acagccatcg agtttgccga gttagataaa aaagacattg tgatcgacgc
    11521 ttattcagga ataggtacga ttggcttgtc tttagcagac agagtggata aggtgtatgg
    11581 gatggaagtt attccagaag caattgaaga tgcacagttc aatgctttaa ataataaaat
    11641 cgaaaatgtt cattatgaag tcggaaaagc agaaaaagtt atgaaaaaat ggcaagaaaa
    11701 agggatcaaa ccatctgtga tcgtagttga ccctccacga aaaggattag atcatcattt
    11761 tattgaatca gcaatcgaaa tgtcaccaga acgaatcgtc tatatctcat gtaatccggc
    11821 aacttttgca agagatgcta agttatttgc agaagcaggt tatgccgtga agaaagttca
    11881 gcctgtagat cttttcccgc aaacccatca tatcgaatta gtcgcttcat tcgttaaagg
    11941 agaataaaaa tggaccaaaa agaaaaaatg aaagaattga tcaaaaagaa acaaggtgga
    12001 ggccgatcca aacaaatgga aacacctaag aatgatcgta aaaacatgag aaaaggccct
    12061 aaaatcttta ataaatagac gtgctaaaga aagactaaga agtgttaact tttgttattg
    12121 tttcttagtc tttttagtta gtctacatta ttttggcgat ttgccaataa ggactgttga
    12181 gttgtgcaaa atcaaatctg ggtcagaatg aacgtcattc tgtctcagat ttttcgttat
    12241 ttcaatttga ttaactgatt gatcaaaaag atttttattt tcatattctc aaatgattca
    12301 acactaaata tacatgtttg acgcggaaat tattattcga ttttggcggc atcagggggg
    12361 atgacgacgt agcagaccca ttattttact caagaaaatg tccgtgccta ttatttagaa
    12421 tacgatagtg aacgtgctgg gagttttgtt cttttagaga aagtctctga agataaatta
    12481 gttgtcttag attttgactg taacgaaaaa tggcgaaatg gaagagcacc agcaagtgat
    12541 tgaccggatc tacgaagcga cacgatatct gcctttggat cgtttgtgct taagtcctca
    12601 atgtggattt gcttcaacag aagaagaaaa tgttttgttt gaagaacaat ggaaaaaatt
    12661 tcttttgtca aaagcattgc agaagaagtt tggggaaaat cattaaatca tctaggagag
    12721 cttgatagga aataacacta tggtataatc agaatgctta atactaacta ctgagggagc
    12781 cttatgaaaa agaaaattct taaatttatc ggatggttct tcggaatcat tcttgggatc
    12841 gcgtttgcaa tctttcttgc cttccagctt tcccccagac ctggagcatt cttgatcaac
    12901 catatgtttt caaatactgt tcaaatcact gataaaaaaa cgttcgatca agcagaaaaa
    12961 aacgtggcga aaaaaacaga tttgacgtat gagtccaagt ataaagagaa tacttatgac
    13021 atctactatc caaaaaacag caaggggcct gttcctgttc tattttgggt acatggtgga
    13081 ggattcgtcg gcggagataa atcgggtgtc aaagaatttg cgacaaaatt agtttcagac
    13141 gccaacatcg cagtggttgc gatgaattat gaattagcgc ctgattcaga ataccctaat
    13201 caagttcttc aagtgaatga actgatcaaa gatttattag ctgaagaaaa agacgacaaa
    13261 ttgctagaca tggaacaatt attttttggt ggagatagtg caggttcaca aatcgcattg
    13321 caatatgcag ctatacaaac gaatgatgcg tatgctaaac aaatgaaaat caaacagcta
    13381 cttccaaaag atagtctgaa aggaaccatt tcttattgcg gacctgttga tttgaagcta
    13441 acagcacagc agcattcgga tgatcgattt atgaagtttt tcgtcaaaac cgttgcgtgg
    13501 tcgttgattg gaacaaaaga ttggaaaacg agtgagcaat tgaaagaagc tagtgtggct
    13561 gaccatgttt ctaaggaatt tccgccaacg tatataactg acggaaatgc atactcattc
    13621 caggagcaag gtcaagcatt agaaaatcgg ttgacagaat taaaggtacc cgtgcaatcc
    13681 ttgttttata cagatacaaa aaaagagatt acccatgaat accaatttga ttacacgcta
    13741 aaagaatcgc aaaattgtta ccagcagaca ttggattttg tgaataagta taagtaagag
    13801 gttgtgaaaa agccgtttcg ttttgagtat gaagaaacga agcggctttt cacaacctct
    13861 ctttcaatac ctttttgttc ttgggataaa actttcccta acaatcaatc gtgcaggata
    13921 agtcaaaatg attggttcgt cccgtatacc gtcaattcgc tccaatgcca ttcgaacagc
    13981 tgcttgccca attgatttcg cattgatttg aacactggtc aagccagggg agaggtttgc
    14041 gattgtctcg atattatcaa agcttgctac aacaatctct tctccaattt ggatattttt
    14101 ttgtttcaat gcccgataga caccaattgc cattggatcg ctcccgacaa gaatggcagt
    14161 aggaagaggg gattcatttt ctaatagatt agtcgcaagt acctcacctt gtttttctgt
    14221 ccagccacct agatgataag acatatattt ttctaagtga tgatcgatca taaaatgctg
    14281 ataggcacgc atcctctttt catttataga aatctgcttc ccaccgaatt catccatatc
    14341 gactttatat cccccgatat aggctattct tttatgacca ttttctaaga acaattccag
    14401 tacggtttta gtcatccgtt caagatctgc atacaccata tcgacctctt gctggatgtc
    14461 agggttatcc acgacgacta tttgcatgtt ttgttttaac aaggcggtaa tcgattcttt
    14521 cgtaacagtc ccaacaacga tcaccgcacc taactgatct agatcttccc attgtttagg
    14581 attatcagat agattataga ttcgattcat ccctaaatgc agacgtcgag cttcttcttc
    14641 catccctact cgcaaatgtt tgtaatagat atcctgttct tcgctcgctt tgccgatcgt
    14701 agagatcaca ccaatcgaag gcattctctt tttgatatat ttacgagtac tgatgtcata
    14761 atccaacgca gcagctgcct ctactatccg ttgccgagta gtctgagcca catggatcgt
    14821 ctgatcgtta ttcaacacac gggaaactgt agcaggagag acatttgcct ttcgtgcgac
    14881 atctctaatt ccaaccattt ttacacactc ctcaaaaaaa taaaaaattt ttattgacaa
    14941 aattgtttag taaacagtat acaggaatta taaaaaaaag taaacggttt ccgttttgtt
    15001 gggagtgaaa tgatggatat tacgcataga aaaacagata aaaatttcat cttatttgat
    15061 gaaaagagcc gagtcttcca tttacgaaat acatttcttt cttatctgat aaagatagaa
    15121 gaaagcaatg tattggcgca tatttatttt ggcaaaccag tcaagcagta caaagacaat
    15181 aaaaattatc cccgaagaga tcgtggattt tcaggaaatg ttcccttaaa tcctgaccgg
    15241 tctctgtcga aagatacact accgcaagaa tactcttctc atggcagcat ggactttcga
    15301 acgcccgcat cgatcattca gcgcaagaat ggatcagact tactggatct gagatatgac
    15361 agccattata ttacagatgg gaagccagag atcgaaggct tgcctcaaac ttatgtgatg
    15421 gacaagtcag aggcacaaac gttggtgatt tcacttaagg atagagaaac agcgatttat
    15481 ttcgatttgt tctataccat atttactgat agagcagtga tcacacgatc tgtaaagata
    15541 agaaatgaaa ccggagaaac aatcaaacta gaaaaagcgg ctagtttcca gctagacttt
    15601 gcccatacta ggcgatttga tgaagtcatt gctttgccag gagcacatgt caatgaaaga
    15661 cagatttcac ggcaatctgt tttatctgga acaaaagtat ttgaaagccg tcgaggaacg
    15721 agcagtcacc atatgaataa ctttattgct ttagttcatc atcatacgac agaaaatact
    15781 ggagaagcga tcggacttca atttgtttat tccggcaacc attctttcga acttgaaaaa
    15841 gatcagatca atcaattgag agtcgtcggt gggatcaaca gccatcgttt ttcgtgggaa
    15901 ttgaatgcag gccagtcttt tcaaacacct gaaatgatcc tttcttactc aagtcaggga
    15961 ttgaacaaaa tgagtcagat ccaccatgag ttgctacgtg aaagaatcgc aagaggtcgt
    16021 catcaatttg ctgaacggcc gattctggtc aataactggg aagccactta ctttgacttc
    16081 aatagtgaaa aaatcaaggc gatcattgat gaagcaaagg agctaggcat cgaaatgttc
    16141 gtattagatg atggctggtt tggaaaaaga gatgcggaca attcttcatt aggagactgg
    16201 ttcgagtatg aaggcaagtt gacaaatgga ttacgtgaaa tagcagatta tgctcatagc
    16261 aaaggattga aatttggttt atggtttgaa cctgaaatga tctcggttga ttctgaactg
    16321 tatcgaacac atcctgattt tcttatgcag gagccaggta gaatgccttc tgcttccaga
    16381 tcacaacatg tactggattt tacaaggtta gatgttcggc agacgattga gaaacaaatg
    16441 agaaagattt tagatacgat tcctcttgac tacatcaaat gggatatgaa ccgttcgctt
    16501 tcagatgtct attcgattac ccttgaccca cagagacaag gagaagtagc gcatcgttat
    16561 atgctcgggc tttatgaact acttgaacat ttgacaactg attatccgga aatcttatgg
    16621 gaaggctgtt ctggtggagg tggacggttt gatgccgggt ttatttatta tatgccgcag
    16681 tcatggacaa gtgataacac ggatgcagtg gagcgtatga aaatccaata tggaacaagc
    16741 cttgcttatc cgatttcgtc catcacagct catgtatcag ctgtaccaaa tcatcagacc
    16801 gacagatcta cttcgttgaa aacaagagga gaaacagcta tgagcgctgt ttttggttat
    16861 gaactggatt tgacaaagct gtctcctgaa gaagaaaagc aagtaaaaga ccaaattata
    16921 tcctatcaaa cgattcgtcc ggtgatccag tatggccatt attatcgttt ggcgagtcct
    16981 tttgaagaaa atattgccgc atggatgttt gtgtctccta aacaagatga agctattgtg
    17041 tttttaggga ggatactagc ttcagctcaa ccagcgttcc atgaagtata tctgatgggg
    17101 ttagatgatg aggcacttta tcaggaacag acctcgaagc ggatattttc gggggccgaa
    17161 ttgatgacag ttggacttta cttccccgat tttcaaggtg atttccaaac agaactgctt
    17221 catttcaaaa agttatgaga gagaaggaaa aaagtatgaa agcaaatata aaatggttag
    17281 atgatccaca agtttttaga gtgaaccaat caaatgcaca tagcgatcat gtttgttatg
    17341 caacaaggga agaagtagaa accacatcct cccttaaaca gtctttaaat ggatcatggc
    17401 gtttttgtta ttcgccaaat ccttcggtac gaccaaaaac attttataaa aaagattttg
    17461 atgcctgttc ttttgatgaa atcactgttc ctagtcatat tgaattagca aattatgaca
    17521 agatccatta catcaatacg atgtatccgt gggagggaca tgtatttcgc cgtccccctt
    17581 tcacgacaag ccctgagaaa gaggcggaag ggatcttttc agctgctaaa gacaatccgg
    17641 ttggttcgta tttgacgact tttcgattag aagaaggtct aaagggaaaa caaatctcga
    17701 ttcattttgc tggtgtagaa caagcaatgt ttatttggtt gaatggtcat tttatcgggt
    17761 atgccgaaga ttcgttcact ccgtctgaat ttgatttaac accttatttg gaagaagaaa
    17821 atattctagc tgtggaggtt tacaaaagaa gtacagctgc attcttggaa gatcaggact
    17881 ttttccgatt ttttgggatc ttcagagatg tcttcttagt tgctcgagga caaaaccatt
    17941 tagaagatct ttggatcaaa cctcgcttag atgacaaatg tcaaaatggc aaacttgaag
    18001 tagatttgtc ttttgagcaa gtagccaaaa acagtcagct gaatgtagcg atactagatc
    18061 ctgatgggaa ggaaatctat caacagcaat atccgttaaa agagcaaatg acgattcgca
    18121 tcgatggatt tgaaaatgtg gcactgtggg ggcattttca gcccaattta tataagcttg
    18181 aattggagac gatagacgaa gaaaaaagag taacggagta tattgactat tccttcggtt
    18241 ttcgtaaaat cgaaatcaaa gataaaatca tctacttcaa tggaaaacgg atgattatca
    18301 acggggtaaa tcgacatgag tggcatccga aaaaaggacg cgccatcact atagaagata
    18361 tggaaaaaga tttggctatc ataaaaaatg caaagatcaa cggcgtacgg acatctcatt
    18421 accccaacca gatcacttgg tattatttat gcgaccaagc aggaatccat gtgatggcgg
    18481 aaacgaacct cgaatcccat ggctcatggc aaaaaatggg cgcatttgag ccttcttaca
    18541 acgtaccagg tgcagtacct cagtggcagg cggctgttct tgatcgagca aaaacgaatt
    18601 tcgaaacatt aaaaaaccat ccaagtattc ttttttggtc tcttggaaat gaatcttatg
    18661 cggaagaaaa cattcgacaa atgaacgatt acttcaaaaa agtagacccc gatcgacttg
    18721 tccattatga agggaatttt ccaaatccag cattcgaaga ttcgatttca gatgtaaaaa
    18781 gctgcatgta tgcgccacca gaagagatca ttgactacct tgaaaataaa gcggaaaaac
    18841 cgttcatctt atgtgaatat atgcatgaca tggggaattc gttaggtgga atggattctt
    18901 acatgacgtt gcttgatcgt tatgaaatgt accaaggagg ctatatatgg gattttaaag
    18961 atcaggcgat ccaagtgact gatccaatca caggcagaga agtgatgcgc tatggcggtg
    19021 actttgacga taaaccaagt gactatgaat tctcagggaa tgggatcgtt tttgcagatg
    19081 gacaagaaaa acccgccatg caggaggtaa gatattatta tgaaaaatac agtaaataaa
    19141 agtcatatgg atacggaaaa agttgcaatc gtcttcggcg actgtacatt aggtgtcaaa
    19201 tcggggaata cgcattatat tttttcttat acaagaggcg gactggaatc gctcaataaa
    19261 aacggaaaag aatggctata ccgggaaaca cttccagttt tttggcgagc aacgaccgat
    19321 aatgacagag gtagcggatt tggatttaaa tccggtatgt ggttaggagc cggattgtat
    19381 ccaaaagtca aagaaataga agtactggtg gacaataaaa agatcgattt gccgattgct
    19441 ccagtaaata atcaatattc aagtaatgaa tacgctgaaa cggttgaaat catttttaca
    19501 ctggaaatca acacagtacc aaaaacccaa gtgacaattt cctaccaaat cgctggagag
    19561 ggtaaaatgc ttgtcaatat gcattattat ggacaaaaag gattaccgga attacccgca
    19621 ttgggtttaa ggttcattat gccaacgaaa gcaaaatttt ttgagtatga aggcctatca
    19681 ggggaaacat atccggatcg gatggctggt ggtgtcgaag gtatttataa agtggcagga
    19741 ctccccgttt ccccatacct tgttccccaa gaatgcggca tgcatatgga aacgaaatgg
    19801 gtagaactaa caagaaatca aacgttggat aatacggata aagatcaaga agatttttcg
    19861 ttgcgatttg aacagcagga tacgccattt gctttttccg ctttaccatt caaagcagaa
    19921 gaactggaac aggcaacaca tatagaagaa ctaccgccag aaagacgaac ggttcttacg
    19981 atttttgcaa aagtaagagg agtcgggggt atcaatagct ggggagcgga tgttgagtca
    20041 ccttaccgaa tcagcagcga agaggaccat gaaatcggct ttattattca gtagcaatct
    20101 ttagattatt atttgaaaaa cggagtttac aagacaaagc tgctttattc tatatgtcat
    20161 gaacaagaga gtaaaaaagt tctcagatac ttttgttcat gactattttc ttcaataaaa
    20221 ccaattgtta tttttctgaa aaacaaagtt aactatcaga aaattaaaaa aagtttcata
    20281 aaaa
//
LOCUS       DAZKOK010000049        19694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000049 DAZKOK010000000
VERSION     DAZKOK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.049"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG90W_001491"
     CDS             complement(<1..210)
                     /locus_tag="ACG90W_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0477858.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG90W_001492"
     CDS             complement(216..446)
                     /locus_tag="ACG90W_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477859.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG90W_001493"
     CDS             1063..1725
                     /locus_tag="ACG90W_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477860.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG90W_001494"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG90W_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0477861.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG90W_001495"
     CDS             5145..5459
                     /locus_tag="ACG90W_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0477862.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG90W_001496"
     CDS             5472..5846
                     /locus_tag="ACG90W_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0477863.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG90W_001497"
     CDS             5856..6200
                     /locus_tag="ACG90W_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0477864.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG90W_001498"
     CDS             6271..7623
                     /locus_tag="ACG90W_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0477865.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG90W_001499"
     CDS             7683..8825
                     /locus_tag="ACG90W_001499"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0477866.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG90W_001500"
     CDS             8850..9104
                     /locus_tag="ACG90W_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477867.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG90W_001501"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG90W_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0477868.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG90W_001502"
     CDS             10541..10678
                     /locus_tag="ACG90W_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0477869.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG90W_001503"
     CDS             10683..10904
                     /locus_tag="ACG90W_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477870.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG90W_001504"
     CDS             10917..11417
                     /locus_tag="ACG90W_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0477871.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG90W_001505"
     CDS             11514..13361
                     /locus_tag="ACG90W_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0477872.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACG90W_001506"
     CDS             13364..14260
                     /locus_tag="ACG90W_001506"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0477873.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACG90W_001507"
     CDS             14310..14699
                     /locus_tag="ACG90W_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0477874.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACG90W_001508"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACG90W_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG90W_001509"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG90W_001509"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0477875.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACG90W_001510"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACG90W_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAZKOK010000050        19544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000050 DAZKOK010000000
VERSION     DAZKOK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.050"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..600
                     /locus_tag="ACG90W_001511"
     CDS             118..600
                     /locus_tag="ACG90W_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477876.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            797..1687
                     /locus_tag="ACG90W_001512"
     CDS             797..1687
                     /locus_tag="ACG90W_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0477877.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2012..4483
                     /locus_tag="ACG90W_001513"
     CDS             2012..4483
                     /locus_tag="ACG90W_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0477878.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4586..6322)
                     /locus_tag="ACG90W_001514"
     CDS             complement(4586..6322)
                     /locus_tag="ACG90W_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0477879.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6319..7023)
                     /locus_tag="ACG90W_001515"
     CDS             complement(6319..7023)
                     /locus_tag="ACG90W_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0477880.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7154..7657
                     /locus_tag="ACG90W_001516"
     CDS             7154..7657
                     /locus_tag="ACG90W_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0477881.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7617..7955
                     /locus_tag="ACG90W_001517"
     CDS             7617..7955
                     /locus_tag="ACG90W_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0477882.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7976..9394
                     /gene="ffh"
                     /locus_tag="ACG90W_001518"
     CDS             7976..9394
                     /gene="ffh"
                     /locus_tag="ACG90W_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0477883.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9506..10084
                     /locus_tag="ACG90W_001519"
     CDS             9506..10084
                     /locus_tag="ACG90W_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0477884.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10088..10609
                     /locus_tag="ACG90W_001520"
     CDS             10088..10609
                     /locus_tag="ACG90W_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0477885.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10688..11242)
                     /locus_tag="ACG90W_001521"
     CDS             complement(10688..11242)
                     /locus_tag="ACG90W_001521"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0477886.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11495..11770
                     /gene="rpsP"
                     /locus_tag="ACG90W_001522"
     CDS             11495..11770
                     /gene="rpsP"
                     /locus_tag="ACG90W_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0477887.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11782..12033
                     /locus_tag="ACG90W_001523"
     CDS             11782..12033
                     /locus_tag="ACG90W_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0477888.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12158..12949
                     /locus_tag="ACG90W_001524"
     CDS             12158..12949
                     /locus_tag="ACG90W_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477889.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13119..13640
                     /gene="rimM"
                     /locus_tag="ACG90W_001525"
     CDS             13119..13640
                     /gene="rimM"
                     /locus_tag="ACG90W_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0477890.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13630..14391
                     /gene="trmD"
                     /locus_tag="ACG90W_001526"
     CDS             13630..14391
                     /gene="trmD"
                     /locus_tag="ACG90W_001526"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0477891.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14527..14874
                     /gene="rplS"
                     /locus_tag="ACG90W_001527"
     CDS             14527..14874
                     /gene="rplS"
                     /locus_tag="ACG90W_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0477892.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15006..16142)
                     /locus_tag="ACG90W_001528"
     CDS             complement(15006..16142)
                     /locus_tag="ACG90W_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0477893.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(16258..16824)
                     /locus_tag="ACG90W_001529"
     CDS             complement(16258..16824)
                     /locus_tag="ACG90W_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0477894.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(16960..17613)
                     /locus_tag="ACG90W_001530"
     CDS             complement(16960..17613)
                     /locus_tag="ACG90W_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477895.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(17663..18091)
                     /locus_tag="ACG90W_001531"
     CDS             complement(17663..18091)
                     /locus_tag="ACG90W_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0477896.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(18096..18470)
                     /locus_tag="ACG90W_001532"
     CDS             complement(18096..18470)
                     /locus_tag="ACG90W_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0477897.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            18774..18959
                     /locus_tag="ACG90W_001533"
     CDS             18774..18959
                     /locus_tag="ACG90W_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477898.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            18972..19220
                     /locus_tag="ACG90W_001534"
     CDS             18972..19220
                     /locus_tag="ACG90W_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477899.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            19251..19421
                     /locus_tag="ACG90W_001535"
     CDS             19251..19421
                     /locus_tag="ACG90W_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477900.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT         6286 a         3510 c         3861 g         5887 t
ORIGIN      
        1 gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc cccctaattt
       61 ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg
      121 agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc tatcgttttg
      181 ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa
      241 ggaagcggat ttgtggtttc taggaatttt ccagctttga ctgttttcta tttagtgata
      301 agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac
      361 ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt gattatcggc
      421 cctaatggtt tgagcgagag tggattgaag gattcagctt attcctccat gtatttcttg
      481 acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac
      541 ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga
      601 tggtggatca tcagtaaaaa agcatatgag ctgcgtaaaa tgcatgtttc tcatatgctt
      661 tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta
      721 tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata
      781 agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt
      841 agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag
      901 tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt
      961 cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg
     1021 aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc
     1081 tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt
     1141 agcagtggtc gggatggcgc caatcgtcaa taaagataca gatgtcaaag atatcaccaa
     1201 acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa
     1261 agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga
     1321 aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac
     1381 agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct
     1441 cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc
     1501 agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc
     1561 tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa
     1621 agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat
     1681 caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat
     1741 gtttcccaca tatacatttt tcctttaatt gtgaaaaata gttcctcata ttttgccgtt
     1801 ttttgagcta ataccgaggg tcagctgatt aaacaccgtt tattcgaaaa aaagagctac
     1861 gacatagaac tgtcgtagct ctttttttgc tatatgacag gaatgaaagg tgtttgcaag
     1921 cttaatgtga ggaacacttt ccatatctgt tctatactgg ataggagaag tttggaaata
     1981 tccagaaaac gaaatcaatc gaggtagcca catgaagaat aaatggataa atcgaagtaa
     2041 aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc cgtctttttc catcgtataa
     2101 agaaggatta acggcagatc aagtcaagga acgaatagaa aaaggtgcag caaataattc
     2161 agtcgatccc acattcaaaa caaatcagca aatcgtattg gaaaacatat ttacgtattt
     2221 caatttgatc tttcttatct tagccatttt gctttgttta gtagaatcct ataagaactt
     2281 gactttttta ccagttatca tagccaatac aggaatcgcc atttttcagg aaattcgatc
     2341 caaaaagatc ttggacgaac taaatgtttt aaatacagca aaagtcaatg tcatacggga
     2401 cgggatagaa caaacaatag atatagaaga ccttgtaata gatgacattg ttattttaga
     2461 gactggacat caaattccag cagatgcaga ggtcgtggaa ggaaaactgc aagttaatga
     2521 ggctttattg acaggcgaag cagatgaaat aaccaaagaa ataggcgatc cattaatgtc
     2581 cggcagtttc attgtttccg gaaaagctta cgcccgatta gacaaagttg gagcagattc
     2641 gtatatttcc cagctgacag tcaaagccaa gacaatggga gaaggagaac agtcggagat
     2701 gattgcttct cttaatcaat tgatcaaatg gattgggatc atcattattc ctatcggtat
     2761 cctgcttttt tctcaaagtt acttttttaa tggaaacacg atcaaagaaa gtgtcgttgc
     2821 aatggaagca gcattgatcg gtatgatacc tgaaggactg tacttgctga cgacgattgc
     2881 attagcatta agtgcgacta aactagcaaa gcaaagagtt cttttacaca atatgaaaag
     2941 cattgagaca ttggcaaggg taaatgtcct ttgtgtagat aaaacaggaa cgatcacaga
     3001 aaataaaatg tctgttcaaa aagtgatcgt ctctaaaaaa caagaaataa tcagtcagtc
     3061 agatcaattg gaagcatgca ttgctgacta tgcaaaagcg atggcaggag acaatgcaac
     3121 aatggaagct gttaaagcat attttacaaa aaccactgat caatcctatc accatgttat
     3181 tcctttttct tctgtccaaa aattcagcag tgtagcgttg gcagacaagg tgtatgtttt
     3241 aggagcgccc gaaatggttt tgcgcgatca attgccggaa tatgcggaag agtttgtttc
     3301 tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg tacgatggga ttcttgaaga
     3361 accagtattg acagaagcag tcacaccatt aggctatatt ctgattgcta atcctatcag
     3421 aaaagaagca agagccacat ttgattactt caaaaaacag caagtagcca tcaaagtgat
     3481 ttctggagat aatccgctca ccgtatccaa tgtagctgtc caagcaggga tttcacatgc
     3541 tgatcagtac gttgatgcat ctaaattatc agaagaagaa tatgaagcag ctgtcgaaaa
     3601 atatacagtt tttggacgcg tcaagccaga acaaaagaaa atcttcgttc aattgataaa
     3661 gcgtaacaat acagtagcta tgacaggtga tggcgtgaat gatattctag ccatgaaaga
     3721 agcagactgc agtatagcga tggcttccgg aaatgaagcc gccatgcagg cctcacaagt
     3781 ggtcttactt gattctgact tttcacgcat gccagaagtt gttgcagaag gacggcgcgt
     3841 tgtgaacaat attgagcgat cagccagctt gtttttagtg aagaatattt tctccttttt
     3901 gctctcactg ttttcagtga tctttgctct gacctatcct ttagaaccat cacaaatcac
     3961 attgattagt ttgtttacta tcggacttcc atctttcttg cttgctctag aagaaaacaa
     4021 aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa aaagctgtgc caggtggact
     4081 aacagatatg atcgtagtcg gtgcgttggt gatctgcggg gtgactttga atctaaataa
     4141 aacagatgtt tctacagcat ctaccatgct tttgattgca gtagggttcc tcgttctcta
     4201 taaaatctgt tcaccgctga ataaattcag aagtcagatc attcttttct gtgctagcgg
     4261 taactttttt tcggtcatct ttttgcataa gctgttttca atcaccggta tctcagctgt
     4321 atcacttttg ctgttgagca ttctattttt ctcggcagat tcgattttta ggcatttgac
     4381 cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa gagcaaaagc cgtttagtcg
     4441 cgtagctaaa aaatttctat cgatatttaa acgagaaaat tgacgaaaag tatctcaaaa
     4501 aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc aggatgagtc atccaacttt
     4561 aaaacgcacg agcagttgct cttttttaca ttttcggaat ggtaacggtg aaagtcgaac
     4621 ctgttccaag atgactgtct acagtaatcg taccattaag gagttgcacg tagtctttta
     4681 caatcgctaa ccctaatccg gtcccgcctg aatgtcggct tcgcgccttg tctacgcggt
     4741 aaaaacgctc aaagatccgt tcctgatcct caatgtcgat acctatacca aagtcttgga
     4801 cagaaaacga tagatcatct gttgcctgat agcggataat aatctcgctg tcgctttttg
     4861 aatattggac agcattttca atcagatttt tgataattgg ataaaggata tctgttttag
     4921 tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa ccgtttcttt tcgatgattg
     4981 gctgataatt ttgaacgatt tgctggaaat aaggttctgt ataaattgtc tggatctcgt
     5041 aagaattctc gccatctttt gacagctgga tgatttcccg aatcagttta tctaaccgga
     5101 tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt ttgcgggtca tcttttgcac
     5161 catccagtaa agtctcggta aaaccaatca gtgaagtcac aggagttttt aattcgtgag
     5221 agacgttgcc aacgaaatcc ttttgtagct tttctaaacg tcgcactttc gttaagtcat
     5281 acgccacccc tagaatctgt ccggtacctt ctgaattagt aaagtagcgc aaactcaagt
     5341 ctaatgtctg ccgaccttct gtaagggtga tctcctgatg gatcagcgga tgctcaggag
     5401 tcacttgatg aatcagctga atgagttttg gctcttgaat gacttctgtg taccgctgtt
     5461 caggttgata gcttaccacg tttaaatgta gctgcatctt tggattaagc agtagtaaac
     5521 gagaatcagc atcatcaatc aggaatacgc caatcatcag ttcgttcagt aacgtatata
     5581 attgctcttc tgtggtagta cacgcccgat acatcttact catttgttcg ctcaacgcat
     5641 taatcgtctg atacaattct tcccattgat cgggtgtttg catgattact tccgcctgat
     5701 taggattttg caccattttt tttagtacgg gcagcacggt tttcaatggc tggttttttt
     5761 gatagatcag ataatagatc attcctgcaa gaacaaggaa gaaagacaga aagaagaaaa
     5821 agacccaacg gcgaaaactc tcagttctcg gtaaaaaacc gcttgtcggt tctgcgatac
     5881 gtaaaatggc ttctaactgg ccactttttt tgactggtaa tgcgacatat aaaagatcct
     5941 cattcaaagt agtacttttg cgcaaagcgc tacctaggtt cccaccattc aatacggctt
     6001 tgatttcggg acgggagttt cgctgttcat tcaatgtttc attgtttgtg tcaaataaga
     6061 tctttccagt agcatccatt aaagtaatcc gttcgttcga ctgatggacg aattcttcca
     6121 aagcagtttt gttttgcgct gcttggaggt tttcgacatc taattgccgg atcagcagtt
     6181 gtgctttttt tgtcaggtag ctttcttgct gttccacgat ctgctgttga aaataatggg
     6241 aaatcaattg ccagccacca aaaaacaaac taactaaaag aataagaaag aagagacgtt
     6301 cataaatttt tttccatttc atcgttttgg ttcctgaaac ttatagccaa atcctctaat
     6361 agtcaccaag tactttggat gttttggatc tcgctcgatt ttttctcgca aatgactaac
     6421 gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa ttccaaatgc ggtctagcaa
     6481 tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa taaacaagta attcaaattc
     6541 tttcggtgtt agttcaatca gatggtcgtc tacggtcact tgataattgg tcaaatcagc
     6601 agtgatttgt ccaatcgata gatattcagg ctcagcttca tcttttgatt gcttacgagg
     6661 ttcaatccgt ctgaatattg ctttcatacg ggctaatact tcgcgaggac taaatggttt
     6721 ggtcaaatag tcgtcagcac ctatttctag gccaatgatc cgatccactt ggtcatcttt
     6781 agccgtcagc atcaaaatcg gtgtttcatt tttttcttga cgcagccgct gagtgatctc
     6841 catgccatcc atcgttggaa gcatcacatc taaaatgatg aaatcaaatg attgttctaa
     6901 tgctaattct aatccttcgc ctccatctgt ggcactggtt accttgtagc cttctttttc
     6961 caaattgaac gtcaagagcg ttacaataga aggttcatca tcaacaacta gtattttttt
     7021 cattcaaatc tcctttaaat aggtttcatt ctgtttatat tttagcatag aatcagtaca
     7081 aagggctatt ttacaaaaga aataaatggt ataataggaa ggcactttgc tgaatagaaa
     7141 ggaagagcag tcaatgattg gaatcagtgc atgtttaggc ggtctggcct gtcgctatga
     7201 tggacagaaa aaagaagtac cagaattgat ggaactagtt gaaactggag aagctgtcat
     7261 ggtctgtcca gaagtgatcg gcggattacc aattcctaga gatcccgctg aaatcattgg
     7321 tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta tggacgacaa gcggcgaaga
     7381 tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat caaaaattgc aggaaaaaca
     7441 cattgatcta gtcatcatga aggaaaaaag cccttcatgc ggaacccatt cgatttatga
     7501 cggaagtttt tccggaataa aaaaaaatgg tgccggagtg gctactgctt attttattca
     7561 gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa actagaggtg agcagaatgg
     7621 aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt ttattcgacc ttattgacag
     7681 aaaaacaaat gaactacatg gaattatatt atgcagatga tttttcatta ggtgagatcg
     7741 cggaagaata cgaagtaagc cgacaagcag tatatgacaa tatcaagcgg acaagcaaga
     7801 tcttggagga ttatgaaaaa aaactccatc ttttttctaa ttatattgtt cgagaacagg
     7861 tattggaaaa tatgactgca tatattacag ataagtaccc ggaagataaa aaactacttg
     7921 aatatgtaca gcaaattcaa gcaatcgaag aataaataaa aggaatgaag aaaatatggc
     7981 atttgaaagc ttaactgaac gtctccagca ggcgatggga aaaataagaa aaaaaggaaa
     8041 agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc cgccttgcct tactagaagc
     8101 cgatgttaac ttgcaagtag tcaaagattt tactaagcgc gtacgcgaac gtgcggtagg
     8161 ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc gtaaaaatcg ttgatgaaga
     8221 attgacgatc acacttggtt ctgaaacagc agagttaaat aaatcaccaa aaataccaac
     8281 tgttatcatg atggctggtc ttcaaggggc aggtaaaacg acctttactg gtaaattagc
     8341 caattattta aagaaaaatg aaaatgctcg tccgctccta atcgcagggg acgtatatcg
     8401 accagctgcg atcgaccaat tgaaagtatt aggtcaacag ctagacgttc ctgtatttga
     8461 tatgggaact gaagtcagcc cggtagagat cgtccgtcaa gggatggagt tagcaaaaga
     8521 aaagaaaaac gactatgtat tgatcgatac ggcaggacgt ctgcatatcg atgaaacatt
     8581 gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca aatgagattt tgcttgttgt
     8641 cgatgccatg actggtcaag atgccgtcaa cgtagctgat agcttcaacc agcaattagg
     8701 tattacaggg gtcgtcatca ctaaacttga tggggatact cgaggcgggg cagcactatc
     8761 gatccgctca gtgacaggcg caccaatcaa attcatcggt tctggtgaaa aattgactga
     8821 cttagaagta ttccacccag atcgtatggc tagccgtatc cttggtatgg gggatatgct
     8881 gacattgatt gaaaaagcgc agcaagacta cgacgagaaa aaagcggaag aacttgcaca
     8941 gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt gaacagcttg atcaagtgat
     9001 gggtatgggc ccactggaag atctaatcaa aatgatccca ggaatgaatc aagtccctgg
     9061 aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc aaaaaagcga tggtttattc
     9121 tatgacgcca gcagaaagag caaatcctga tctgttgaac ccaagccgtc gtcgccgaat
     9181 tgctgccggt tctggaaaca gtgttgttga agtgaatcga atgatcaaac aattcaagga
     9241 atcaagaaaa atgatgcagc aaatgtccaa aggggatatg aacatcccag gtatggacca
     9301 aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg gcgatgaatc gaatgatcaa
     9361 gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa aaaaacagct tcggtacaga
     9421 ctgaaaatgt ctactgccgg agcttttcta tcagcaattt ttattcttta ctaatacaat
     9481 tactctaaac gaagaggtgg cacagatgag atgtccgtgg agtgaacaaa cagaaagtat
     9541 gcggcaatat catgatactg tctggggtgt tcctgaatat gatgaccaaa agctgttccg
     9601 aaaattgatg cttgatatta atcaagccgg attgagctgg cagacgattt taaataaaag
     9661 tgaagcattc gatgaagcat atgatcgatt cgatatcaaa aaagtcgcaa attatggaga
     9721 aacaaaaatc agtgaactaa tggaaaacaa aggaattatt cgtaaccgaa gaaaaatcga
     9781 agcagccgtc caaaacgcca aagcagtctt gaaaattcaa gaagaaatcg gcagtttttc
     9841 agagtatctt tggtcgtttt ctaatggaaa aatcgttgat tcttcttatc attcacaaga
     9901 agaagtccca acaacaagtc ctctttcaga tgaattagca aaagatctaa agaaaagagg
     9961 atttaaattt atcggaagca caacaatcta tgctttttta gaagctgtcg ggatcatcaa
    10021 tgatcacctt gacagttgtt tccgcaaaga agaggtcgta cgtatcggca gaaagggcga
    10081 ataaagcatg cgaaaaatca tattttatgg tgctataagt ttagatggct atttggcaac
    10141 gaaaacggat gatcttcaat ggctttttga cactccaaca ggtaagcaaa ctacttatga
    10201 agctttttat gaaacaatcg atacgaccat catgggcaga aaaacttatc aggaagcaaa
    10261 aaaatatttg gagaccgaag caatttatcc agagaaaaag aatttcgttt tttccaccca
    10321 cgcacaatta caattgccag atgctgaggt cgtaaacgaa gatcctgttg cttttctaaa
    10381 aaatctacaa atgacagaag gaaaagatat ctgggttgtg ggtggcggtc gattgatcaa
    10441 accgctattg gaacatcaaa tgattgatga atggtatatc cagattaccc ctgtattatt
    10501 aggggacggg attcctttat tccaaaaagg aacatatgaa gcgcgttttg aaatgctaga
    10561 cacgacacga ttcggtgaat ttatcgaatt gcattatgta cgaaagtaga agtaaataaa
    10621 ataaaaaagg caactatatg cagaaatgtc ctgtacatag ttgccttttt tattaccgtt
    10681 atgcttttta ctctgttaat ttgttagaaa aatctagata cgcatctaaa atcgtttgga
    10741 agaaacttaa tgttccttca acgatattac cattttcatc aattagattc gcaacgtttc
    10801 cgatatatgc ttccggttgc tgcaaggttg gcacattcaa aaagacaagt gattggcgta
    10861 gatggtggtt tgcgccaaag ccgccgatcc cacctggtga tgcagtaacg actaaacctg
    10921 gtttatgatc ccaaacactg tgtccataag gtcttgagcc tacatctaaa gcatttttca
    10981 atactgctgg cacagaacgg ttgtattcag gagtaacgaa aacgacaccg tctagtcccc
    11041 tcacttcctc acgaaaacga gtccattcag caggagcctg ttctggagtt tcgagatcct
    11101 cattgtaaaa tggcagatca tcgattttta caaatacact ttcgtaccct tcaggaagca
    11161 atttcgcaaa agcttctgct acttttttgt tgtaagaatc ttttcttaaa ctaccaataa
    11221 aaaatccaat tttttttgtc atacgatttt tcctccttca aactatctat aataatctta
    11281 caaaagataa gtaactggag caaataaaaa cacttactga caaaagctac atagaagata
    11341 gattacttat ttttaacaaa aaagaagtct aaaatcttct attttctaaa aaaaatcgaa
    11401 tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa aaaaaactgt tacactacat
    11461 cttgtgaata aaacaatgga ggtggaaata aataatggca gttaaaatcc gtttaaaacg
    11521 tatgggttct aaaaagagtc cgttttaccg tatcgtcgta gctgattcac gttctcctcg
    11581 tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct ttgaaagacc ctgcagaagt
    11641 agttttaaaa gaagacttag ttcttgactg gttatcaaaa ggtgcacaac cttctgacac
    11701 tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa caccacgaag ctaaattctc
    11761 aaagaaataa ggtgaccgga tatgaaagat ttaagcgact tagtcttaac aatcgttcgt
    11821 ccgctagtca cttatcctga tcaagtcaag ttagacgtcg tagagtcgaa agacttttac
    11881 gagtacaatc taacagtggc tcctgaagat attggccgta tcattggaaa acaagggcgt
    11941 gttgctaaag caatccgcac aatcgtttac ggtgttcgga tcaatgcacc gaaaaaagtg
    12001 cgtttgaata tcatcgatga taaggaaaag taaataaacg tgctgtactc gaattttcga
    12061 gtgcagcttt tttgtttttt ttattaattg aagaaatgat tatttggcaa aacaagcaag
    12121 aaagcgtatc cttataggag gagggagtga acagccaatg agaaagaaaa tgttatttat
    12181 tccatttgct ctattgttaa gcgcttgtag ttcggttact caagaatcag acaatacaca
    12241 tctatcaagc agtatatctg aaactgttac cagtccagaa aaaagtagta ccaaaacggc
    12301 ttccaccact gaacaaacaa tggctacctc aaataatgag aaaaagactg ctttagacca
    12361 gttgaaagaa caacagccaa atgttcccat gcctctagat gtaccagtct cttcaggcta
    12421 tctcaatatc gcagcgacac atacaaagca aggttattct attttatatt acagaacaga
    12481 tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa acgcctattg caacctatct
    12541 atatcaatat gggtttgctt ctagtcaaga aacaatccaa gttttgcagc catttgagat
    12601 cgacacaaat ggtcagcaag tggacttagg ttctcgaatc acagggtatc agcaaggtgc
    12661 agcaggttca agttttcttg aatggcaaga aggaaactgg cgtattcgaa tccgtgggaa
    12721 taatatagaa ggccaagatc ccttactatt agctaaagaa atcgtagctt atttggaaga
    12781 aaacagtcta ccagcacctg aacaatttgg taaaattact gttgatatgg gtgatacgac
    12841 gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat gcctatacga tcactcatca
    12901 agatcctatg tctgcgttga agatggcagt ttctatgaag aggttgtgaa atcagcgcgc
    12961 tgatctgagc ccatcattta actccaagtc acaggaagta atcaaaaata gcacgccgca
    13021 tttttgcata attttggctt aataccttaa aagaaaacta gaattgcctc ttcacacatg
    13081 ctaaaataaa acagacaaaa aagaaatggg gatataagtt gactgaatac ttgaatgtag
    13141 gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg tgttatctca acaacggatt
    13201 tcccagaaga acgttacaaa aaaggaacgg tgctaacact attccaagac ggaaaagcac
    13261 ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa ttttgactta ttgagttttg
    13321 aaggacatcc ttctatcaat gatgtggaaa aatatcggga tggcatatta aaagtgtcaa
    13381 aagacaactc tgtagatcta gctgaaaatg aattctatta ccatgagatc atcggtctgc
    13441 atgtattaga agatggaact gagttaggta aagtaaaaga aatcttgtca ccaggtgcca
    13501 atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat attgcttcca tatatcgctt
    13561 ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg tgtagaaatt ccagaaggac
    13621 tgattgacga tgaagattga tgtattgact ttatttccaa gaatgtttga aggtcctatg
    13681 ggggaatcca tcatcggtaa ggctgtagat aaaggattat tagatatcaa tatctctaac
    13741 ttccgcgatt attcagataa caaacatcag acagtagatg attatccata cggcggtgga
    13801 gcaggtatgc tgctcaaagt ccagcccatc tatgataaca tccaagccat tgaaaaagct
    13861 gcaccggatg tcaaaaaaag agtaattctt cttgacccag ctggtaaacg attcgatcag
    13921 aaaatggcag aagaattttc acaagaagaa cacttagtat tcatttgtgg tcattatgag
    13981 ggatatgatg aacgtatccg cacattagtg acagatgaag tttccttagg agattatgtc
    14041 cttactgggg gagaattagg tgcgatggtc atgatcgatg caacagttcg gcttttgcca
    14101 gatgtgttag ggaatcagac ttcagctcaa acggattctt attcaaccgg tttattggaa
    14161 catccccaat atactcgccc ggcagaatat aaagggatga aagtaccaga agtattgaca
    14221 aatggaaatc ataaattgat tgaagaatgg caattaaaag aatcactaag aaggacctat
    14281 caacgtcggc ctgatctttt ggaaacactt gaatggacac ctcaaatgac gaaattttta
    14341 gaagaaatca agaaagaaga acaagaaaaa acagcagatg aagccaagtg actttttcta
    14401 aaatcttctt tacagtaggt tgataatatg ttagtatttt atggtgagtg caaaagcact
    14461 taactattac gatattccgc tgtgagaaaa gctcataaga atatttggaa taaggagaag
    14521 aaaaccatga atccattaat cgaagaatta acaaaagaac aattacgttc tgacatccca
    14581 gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag ttgttgaagg tactcgtgaa
    14641 cgtatccagt tatttgaagg tgttgttatc aaacgccgcg gtgctggaat cagcgaaact
    14701 tatacagtac gtaaagtttc taacggagtg ggcgttgaac gtacattccc attgcataca
    14761 ccacgtgttg caaaaatcga agtagttcgc tacggtaaag tacgtcgtgc aaaattgtac
    14821 tacctacgtg cattacacgg aaaagctgct cgtatcaaag aaatccgtcg ttaatcatcg
    14881 ttatcagaca gagaaagaac ccttggtatt caagggttct ttttttatta tctgtttttt
    14941 tatattaacg atttctattt ggtacatttt tgattaataa aaaggggcaa aaaaggggca
    15001 acaagttaca gtttttctat atcttctttc aatttttctt ttactccttt tgtcacatgc
    15061 aaataaattt tttcagtgat atcactattt tcgtgaccaa cgcgatcctg aatggcatac
    15121 agaggagttc ctagttccgc taattttgaa atatgggtat gtctaaaaat atgtgaacta
    15181 agttttttat caatcttcat gtcagcttta tgacttctca aataggtgtt aattgctgtt
    15241 agttggaaag gagttccttt cgttgtttga aatagaaatt gaccatttgg atttaattct
    15301 agaagctcat tgtaaatagc tatcgctttt tttggtaaat caatttctcg cattccggca
    15361 gctgtttttg tagaatcaga ttttttcata tcagctattg aacgttctct atacatcatc
    15421 gtcccattta taactactga tgcattatta ttggtaatgt gtacatcatc tttacttaat
    15481 gcgattgctt caccagacct catcccagtc aaatatagcc actggaaaag gagagaatat
    15541 cttttattgt gtgaagttgt gaaatctact agtctattat attcatcatc ttctaaaaat
    15601 ttatctttaa tttttatcgt ttttgactct cttttataat caataactac ttcatcgata
    15661 ggattttttt ctacataacc ttttttcata gcatatgaaa aaagaagatt caatttggat
    15721 ttaattacac tgacgtactt atttgacaag tcatctttat atatcatgtc ttcaaaaata
    15781 ttattcaaat ctattgtatt aataccagaa acgatgtaag tctcaggtat cttcttcttt
    15841 atagtgttta atatattatt cgtaggataa tatgtagatt ccttaacttg tcttttgtat
    15901 ataactaacc attcttcaac aagctcatga aatgttagat caggcttatg aagtgttttt
    15961 tcgtttaatt ttatatcaat ttttttattt aactctagca atgccacttt ctgagtttct
    16021 cgagatttat ttttataagt aatgctcact ttttttcttt ttctagtttt tggatcaata
    16081 tatcgctcat tatatttata tacttttttt ccgtttttat cagttttagt ttcgatccac
    16141 attgttttca tctcctattt gttgctataa taggaataga taagtaagcc gattatagca
    16201 ggtttatttt tcatcacgtc cacaaacttt ggcgagggag gggcgtgttt ttgttagtta
    16261 ttgtggtagg ttatctaaag cgtattgtgc ttgctcattt gtaaaacctt caaatataag
    16321 ttgatcgtac aattgcgaat ctgacattga tgcccaatca tcgtaacttt ccgctttagc
    16381 caaagcttgt tcgttgtaat cgacatcaac attagccagc gcataatcga ttgcatcact
    16441 tggatattct tcaaactcta gttgttcacg caaaccttgt tcagacatac cagcccaacc
    16501 tagatagctt tcagctttgc tcaatgcatt tctgtattca cgaggaacac tttcttcttg
    16561 agtaacttct tctatagtag tttcagtagt ttcagtttct tcttcaacga ctgtagattc
    16621 tgctaccgat tctgcagaag acacttctgt cacgctttct ttagttgttt cagcgacggt
    16681 atttttagca tcatcactgc ctccttgcga acctatagca ataaacacca caacaaccgc
    16741 taacaaccaa aaccataccc gtttataaaa cggtttctta actttataca ttttcccatc
    16801 ttgacccata actttttttg ccatttaaat atccctcgct tcttgttata atatatttgt
    16861 gatctcagaa atgaggtatg agtccgtgtt gcagcacggg cttttttctt tataactttt
    16921 ttagagatta taggcaaaat agtagggcat aaaaatgtat tattgaattc cgtatttaga
    16981 aaatcctaat tgaacttcac cggaagtctt ttgctgtgtt gtacgcaatg cttcttcttc
    17041 agacattcca ttctgtactt tccatgcaac aggcgacatc ccgtatttgt taacaaaatc
    17101 agtaagtgat aaagtgtcag cgtcttgctg agcgcttgtt tgttggtctt ctggattctg
    17161 ttgagatgct gcttgttgtt ctttctgatc ttgactgata atattgccag catcatctgt
    17221 agtcaatcca ttttcataaa gggccacgcc gaaagcttcc cactctttgt tggaccaatt
    17281 tgcacgatca gctggagttg actgtaaagt gcgttgtttc atctgttcat atgtttcttc
    17341 ttgaggtgcg gtttggattg tactctgact ggagcttata actgttgggc taggttccgc
    17401 cgtagcttgg ttggagcttg taactgttga actagaatct gttttagatg tagatttgct
    17461 agtagatgaa ctggtttcag ttgtttcttt tgttttactt atttttgttt cttggttaga
    17521 agtggtatct gttgattcag cttttttatt atttgaacaa gctgaaagta gcagagcagt
    17581 acttaacaac aacataacgc taactttttt cattttataa ttcctctttc tcgttgtaat
    17641 atgtgtgcta acacgggctt ttttatataa gaaaacgata agcgctttct ggaagcccgt
    17701 aaagattctt taattcctcg atttttttag gatattgatc attatcttct ttataaagag
    17761 aaacaatgag attagcagca aagcaattag cttcgctttc agatttgctt ctagatgttc
    17821 ttgttgatac ataatagctg gataagccac gatgaaaaat agcgtgacct aattcgtgag
    17881 cgcaaatgta gaatctttcc tcagagtctc gcagttcatc atttaaaaag attatcgcac
    17941 gatctctaat ttcttgaaac tgccccttgg gattctcgat aaaaggaacg tattgaattt
    18001 taatgcccat cttttcacaa atataaaaag gattagcgga ctggtatttc cgcttcaact
    18061 cctcgactaa attaatcgta tccatctcca taagctcaca tctttttgcc tttttctttg
    18121 tcttctttca caatatccca gaaagtcgct atcaggatat cttttacgcg ctgtatttgt
    18181 tcgggtgtca atgtttcccc accataagac atattaacat ttgagtctag tagtttatca
    18241 agttcaacca cttcctcttt tgtagcccac ttgggaacat tattatttcc caataaataa
    18301 tcagttgtga caccaaaata atcagcaact ttctttaagt tctcagattt tggcgaggct
    18361 ttatcccatc ttcttatttg tccattagag atgcccacct gtctttctac ttctgctata
    18421 gtcacatgct tttcgtctgc taattcttta atcttagtaa ctaaactcat tattatcaac
    18481 ctttcaaagc tgaaaagaaa ataaatagct taaaagttat atttttggtt gacaattagc
    18541 ttttaagcta gtatatttaa ttcgtaagct aaattgttag ctaaataaga gcaacaaaaa
    18601 actctactaa tttaaaacat tctctcggtc gccaaactta gaaatgttat tttagaggct
    18661 ttttataagt cttatttaac tatgtattca taatagctta aaagctaata ggtgtcaacg
    18721 atttagctaa ttttttagct tacaaattat ttgtttagaa aggagctatt tttatgtctg
    18781 agaatttaga cttaaaaatt cgagcggaga tgagaaaaag aagaatgact ttcaaagaac
    18841 tagctgcgct tatcggtatt tcaggagctt atttatcaga tattctaaac ggcaatcgtg
    18901 atggaaagaa agcacaacag catatcgaaa cagtgaaaaa aatattggac atccgatagg
    18961 gggggtaggc gatgacaaaa ctaaaaaaac aagattttgt aaaaaaatat aattattctc
    19021 catctactta tcaacgtcga atgtcggaac taaaaaatac agcaattttc tcagcggcgt
    19081 atgaacgggt cacaggacaa gaagtttgga tcaatacaga attatacgat aaatttttgt
    19141 ctttcaaatc ctataacagg ttacgcacaa gaaaggtaac acctaaagaa tttatcgaga
    19201 agcatttagt tgatttataa aaaacagaac attttgagag gtgaaggtta atgggtaaat
    19261 tcaacagagc attagtattc agcgcaccgc taatcgtcta tgctttaggg ctttggggaa
    19321 gtagacaagc gttgatagga acgattgtgt acatggtttg gatttttata gggctggatg
    19381 aagctgagta caaaacaaaa aagccagtcg ggagggactg actaatgagt aaggacgata
    19441 ttgtatttct gttaattatg ttttgtggga ttggatcaat aataattaat ttgtatttga
    19501 ttattaaaga aaaatttttc aattaaaatg agtcttttag cttt
//
LOCUS       DAZKOK010000051        18930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000051 DAZKOK010000000
VERSION     DAZKOK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.051"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..2134
                     /locus_tag="ACG90W_001536"
     CDS             2..2134
                     /locus_tag="ACG90W_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0477901.1"
                     /translation="MQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTDLEGTSAF
                     GLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKTKGNVLWI
                     ADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWKQKGLVFH
                     IVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVLFEYSRSY
                     LLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDALASATLSI
                     FLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEMAAGAAVN
                     SSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIKHLIQLIS
                     SALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQVANNRLNE
                     IFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPSHSKTAIV
                     GVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQESFFFSGTI
                     IENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGGQRQRLAL
                     ARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAKACDQILV
                     LHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2103..3518
                     /locus_tag="ACG90W_001537"
     CDS             2103..3518
                     /locus_tag="ACG90W_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0477902.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3551..4585
                     /gene="sppA"
                     /locus_tag="ACG90W_001538"
     CDS             3551..4585
                     /gene="sppA"
                     /locus_tag="ACG90W_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0477903.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4587..5108
                     /locus_tag="ACG90W_001539"
     CDS             4587..5108
                     /locus_tag="ACG90W_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0477904.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5105..5491
                     /locus_tag="ACG90W_001540"
     CDS             5105..5491
                     /locus_tag="ACG90W_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0477905.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(5987..6059)
                     /locus_tag="ACG90W_001541"
     tRNA            complement(5987..6059)
                     /locus_tag="ACG90W_001541"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6024..6026),aa:Lys,seq:ctt)
     gene            6440..7009
                     /locus_tag="ACG90W_001542"
     CDS             6440..7009
                     /locus_tag="ACG90W_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0477906.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            6996..7856
                     /locus_tag="ACG90W_001543"
     CDS             6996..7856
                     /locus_tag="ACG90W_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0477907.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8046..8750
                     /gene="yycF"
                     /locus_tag="ACG90W_001544"
     CDS             8046..8750
                     /gene="yycF"
                     /locus_tag="ACG90W_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0477908.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8755..10590
                     /gene="walK"
                     /locus_tag="ACG90W_001545"
     CDS             8755..10590
                     /gene="walK"
                     /locus_tag="ACG90W_001545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0477909.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10587..11903
                     /gene="yycH"
                     /locus_tag="ACG90W_001546"
     CDS             10587..11903
                     /gene="yycH"
                     /locus_tag="ACG90W_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0477910.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11904..12773
                     /locus_tag="ACG90W_001547"
     CDS             11904..12773
                     /locus_tag="ACG90W_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0477911.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12828..13637
                     /locus_tag="ACG90W_001548"
     CDS             12828..13637
                     /locus_tag="ACG90W_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0477912.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13740..14369)
                     /locus_tag="ACG90W_001549"
     CDS             complement(13740..14369)
                     /locus_tag="ACG90W_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0477913.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14539..15831
                     /locus_tag="ACG90W_001550"
     CDS             14539..15831
                     /locus_tag="ACG90W_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0477914.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15907..16569)
                     /locus_tag="ACG90W_001551"
     CDS             complement(15907..16569)
                     /locus_tag="ACG90W_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0477915.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16794..17078
                     /gene="groES"
                     /locus_tag="ACG90W_001552"
     CDS             16794..17078
                     /gene="groES"
                     /locus_tag="ACG90W_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0477916.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17129..18754
                     /gene="groL"
                     /locus_tag="ACG90W_001553"
     CDS             17129..18754
                     /gene="groL"
                     /locus_tag="ACG90W_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0477917.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6444 a         3058 c         3922 g         5506 t
ORIGIN      
        1 tgtgcagcaa caagatgaaa aagattgtgg tgttgcttgc ctttcgatga ttttaaaata
       61 ttttaaaact gatatcccga ttcataaatt acgggaccta tctgggactg atttagaggg
      121 tacttcagca tttggattga aaaagacatt tgaaaaattg tcttttgatt gtatggctat
      181 tcaagcagat agtgagatct ggaaagaaag agatcttatc ttaccattaa ttgcacatat
      241 attggttgat aacagctata tgcactacgt tgtaatttat aaaacgaagg gaaacgtact
      301 atggattgca gatcccgcta atggaaaaaa tagaaaaacg attgaagcgt ttcaaaaaga
      361 atggacaggt attcttttat tacctatgcc tagagaaact tatattccta ttaaagaaaa
      421 agtagctgga ttaagttctt ttttgcctat aatttggaaa caaaaaggat tagtattcca
      481 catcgtttta gcatcgattt tcattacgtt ttttggcata ggaagctcat attattttca
      541 aggtattctt gattttttca tccctaatca agcacgttca actttaaata taatttccat
      601 tggcttgatc gctgtctatt tatttcgggt attgtttgaa tatagtcgta gttatcttct
      661 tattatttta ggacagcgaa tgagtatggc aattatgctt cagtatttca aacatgtgtt
      721 aactttacca atgaattttt ttgctacgag aaaatcaggg gaaattattt ctcgttttct
      781 tgatgccagt aagattattg atgctttagc aagtgctacg ttatcaatat ttttagatat
      841 tggtatggtg ttgcttgttg gggttacgtt agctattcag aatagtacgt tgtttttaat
      901 cactctatgc tctttgcctt tttatgtagt tgcaatattc gctttcgtga aaagctatga
      961 aaaatcgaac agggaagaaa tggcagccgg tgcagctgta aattctagta tcatagaaag
     1021 tcttaaagga atagaaacaa ttaaagcata tagcggtgaa gagaaagtat ataaccgtat
     1081 agaccatgaa tttgttaaat taatgaaaaa atcatttcgt acggttacgc ttgataatat
     1141 tcaacaagga attaaacatt tgattcaatt aattagcagt gcattaattt tatggctagg
     1201 ttcttatttt gttatggata atagaattag tctaggtcaa cttatcacat acaatgcttt
     1261 actagtattt ttgacagatc ctttacaaaa cattattaat ttacaagtga aaatgcaaac
     1321 ggctcaagtt gcaaataatc gtttaaatga aattttcgcg attgattcag aaaaatcgaa
     1381 ccagtctttt gaatatatgc tttctgaaca attatttcaa caagatatca ctgtaaaaaa
     1441 cgtttcattc tcgtatagca tgaaggcccc aacgttgaac gaaatttcat gtacaattcc
     1501 ttcgcacagt aaaacggcta tagttggagt tagtggttct ggaaaatcaa ctcttgcaaa
     1561 gttatttgtt cgtttttatg aagcatcaga aggatcgatc cattatggaa acattaatca
     1621 cttagacatt ccttttcaga tattacgcga tcgagtgaca tatttgcctc aagagtcatt
     1681 tttctttagt ggaacaatca ttgagaatct tatattcggt ttagatagcc caccaagttt
     1741 cgaacaaata atagaagttt gtgagatagt ccaactaaat gaatttatta atcaacagcc
     1801 attacgttac gagacattgt tggaagaagg tggaaataat ctttcaggag ggcaacgaca
     1861 acgattagcc ttagcacggg ctttattaaa aaatccggac atattaattt tagatgaagc
     1921 aactagtgga ttagatacct tgttagaaca tgcaattatg aattatctcc tcaatttaaa
     1981 agaaaaaacg attattttta ttgcgcatca tttaccaata gcaaaagcat gtgatcaaat
     2041 tttagttctc catgaagggc atttggtaga aaaaggaacg cacgatgaac ttcgttttgc
     2101 cgatggtatc tatcaaaaat tatgggaaat ctaggagagg gaaacaattt atgcagaata
     2161 ataattggtt cgaacattct agtatctata ctaaacaaca tcatagcttc taccgttggg
     2221 tgttataccc tgtaattctt tttttatgta ttattggatt gtttttaacc tttgctaaaa
     2281 aagaggttgt tattcggaca acggctcaat tgaaccccga aaaaatagag aagttacaag
     2341 tgccattaga agctaaaata atggaaaact tcctaaaaga aaatcaattt gttcataagg
     2401 acgatgtgtt ggttagattg gattgttctt taatagagaa tgaaaaggct cagatagaac
     2461 aagaaaacca acgtattact caacaaataa agatggctca gctatttatt gaaagtataa
     2521 gtaaaggaaa aaatttgttt tcaacggatg acagttttgg ctacagtaat caattaaaga
     2581 gcatgttgtc agaaaaagaa tcactccgct acgctttgaa gcaaagtgaa ttaaatgatc
     2641 aaaagcaatt agaagtatac gaaaagacaa aaagacaact agaaaaacaa attgagagtt
     2701 cagatagtaa attacaagaa tggcaacaag tacaggtagc ttggagtaat aatcaatcat
     2761 taaaagattt ttcaaaagaa atgatggcaa actatgagaa ttggcaagaa caactaaata
     2821 atgtttctga tgatcaaaaa aatcaagtga aactgacaat ttcagcaagc ataaatgaac
     2881 aaattgagca actaaaaaaa gaagtagaac agtatcagtc agaaaaagct aaattagtta
     2941 aaccaactac ttctgagaat gacagaatta gtcaaacgga aaaaggaaag caagagctag
     3001 aacaagcaat aacaactgta aaacaaacag ttacagagct aaatgaaaaa caagagaaga
     3061 accagtcagt tatcaaaagt ttggacgatc aactttcaaa aggaatacta aaagctcctg
     3121 tatctggaac ggttcattta aatgaagaaa caaagggaca agcagaacta gccaaaggaa
     3181 cagtgcttgc agaaatttat ccgatccacg aaaaatcaaa aatgaaattt acagcacttt
     3241 tacctgcgaa tgaaagtacc tatattaaag aagggatgaa ggttcatttt aaattggatc
     3301 aaaaaggtgg ggctccgatt acgatcgacg gaactttaga tgaaatttca gaaaattcta
     3361 ctgccactga gaaaggtgtt ttttatgcag tgaaaggaac gttaaaaaca actcaatcgt
     3421 ttccgtttcg ttatggatta actggagagt tgtctttaat tgtaggagaa aaaacatatt
     3481 ggcaacaaat tgtcgatgtg tttctaaata aaaaataatt atttttacta agaatatagg
     3541 aggagtttat atgaataaaa gaagatggat tgctgtggga gtagcgtgcg gattattgtt
     3601 actatccatt atcgtcgcgc taattccagg aaaagataaa gaggaagcat caaatacaac
     3661 cctaacagga ataaataaga ttttttacgg atctaacgaa atcacagaag aaacgttaga
     3721 agaaggcgca tcaaataaaa agattgtcaa actatccgtc aatggagtta ttgcagatac
     3781 aggtgaaagc aatcttttta gtagggaaca atataaccat cagaattttt taacacagct
     3841 aaagaaaatt caagaagaca aagcggttaa gggagtgtta ttagaagtaa attcacctgg
     3901 gggaggaata tatgaaagtg ctgagattgc taaagaaatg gcaaaaatta aaaagctaga
     3961 tattcctatc tatactgcat ttaaaaatac agcggcaagt ggagggtact acatttctgc
     4021 tggttcagat aaaatatttg ctacggaaga aacaacaact ggatcaattg gtgtcattat
     4081 ttctggttta aattattctg gcttattaga aaaacttggc gtaactgatg cgacgtataa
     4141 aagtggtgca ttaaaagata tgatgtcacc tcaacacaaa ccttcagagg aagagaataa
     4201 agttatacag gaatttgtga tgagtgctta tgatcgtttt gtcaatgttg tagcaaaagg
     4261 aagaaatatg gatactaatg cagtgaaaga acttgcggat ggaagaattt atgatggtaa
     4321 ccaagcagta gaaaatgggt tggtggatca aattggttac tctgaagatg cattagattc
     4381 cctaaaaaaa gagaagaaat taactgatgc gacggttata gaatataaaa atgacacaac
     4441 tggctttgct tcttcttggt taggtaataa gattgctgaa tggcaagggc taaaggcaac
     4501 tacatcagat cgtgtaattt cagtttttga aaaacttgga ttttctgata ctccaaaacc
     4561 aatgtattac tatggagggc tataaaatga ataagaagag tggcattgaa actttgccta
     4621 attatgtatt tgcgggattt tggttacgat tttttgcttt tcttattgat ttaatttgta
     4681 ttagcatcat cacccatgct actataggtc tcttttacaa aatcatggga ctggaatctg
     4741 acaaatccat tattactttt tatggtatag cgtcattagc tatttattta acctattttg
     4801 tacttttgac taagctgaat tcaggacaaa caattgggaa aatgattttt gggataagag
     4861 tagtgagctt tgaggaaaaa gagctaagtt ggcaaactat ctttattcgt gagtgtatat
     4921 gtaggtttat attaaaaaat tttgtatttc aaattgggta tttggtggct gctttcacgc
     4981 ccaagaaaca acatattggg gattattttt ctaacacgtc agtggttgtt attcgttcat
     5041 tagaagctat taactatggt caaaaaattt ctaattctaa aacacttaat ctggagagaa
     5101 ttaaatgaat aagagaaaaa cagagtttat tttgatttta attagtggaa taacagggat
     5161 tttatgtagt cttttagcat tgatgggtgg tatgtttcta tcttctattg attcaactac
     5221 tttaaatact acagaactaa ccagtaagga tatagatatg ttagtcaatc taagcgggaa
     5281 tgttgtttta tttggaggaa ttaccctagt tttttcgata atgattttca taataggttt
     5341 tttagtaaga aaaaatcaaa aaacagtggt ctatggtatt agtttactca ttttatctat
     5401 tattccattt tttcttttaa cattattgtg ggtaattcct ggagttttag gtattattgc
     5461 gggattgatg cttctaataa gaaaatctta attaggcaat gtgtagacaa cgtgaaacga
     5521 ttaatcaatt aaaagctaaa tacgttttga gaaatgataa cttgtagtcg atggaagaca
     5581 tgtagataaa aaaactacgt gtcttttatt tattctaatt ttttcgaaaa aaagacactc
     5641 tctgattgtt gtattaatgg aaaaggggaa agcagcaagt aaatttagtt tttctattaa
     5701 atattcttca ctttttaacc ttttatagta cttagattta atcctatatg tttagagagt
     5761 tcattggcag catgattttg caaagcttct cgaattattg ttatattaga aactttactg
     5821 ttcataatta tgcttctttt ttaactcaaa gtccaaaggg ctacagtgga ctttgagtta
     5881 aaaataaaaa gccctagtta ttttgaaaaa taaaaaaaga acagcgtaaa tgttttcgaa
     5941 atgctttcgc accaacattt acctgttccg tgggtttggc acccaatggg ccgtgagggg
     6001 ctcgaacccg cgacccgctg attaagagtc agctgctcta ccaactgagc taacggcccg
     6061 tacaaaaagt acttaataat agtagcacta agaattttaa aatgcaagac tacatttaaa
     6121 gaaaatcaga aaaatcttta cttccttata ggaaatcatt catagattat tcacatttgc
     6181 tcgctattat ataagcatac caacaaacaa agttcttgca agagaacaaa ccctaacact
     6241 tttttcattt tttactcctt tcccgccgtc tccccggcgg gtctcctgtt cttaacgata
     6301 aaagacaaga aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc
     6361 acctagttaa agcaactaga ttgaatgttt atcataagtc tgatatcatg tttatgaaat
     6421 gaaaaaagga gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga
     6481 actagaaaca ttccgtctgc ctcactggga agaactccct gacttagaac tttatatgga
     6541 ccaagtcaga acgttagttg atcgttatct ttcacctgtg atacaaggag aaaagcatcc
     6601 attgcttacc tcttctatgg taaacaatta cgttaaacta ggtttgatac cagcaccagt
     6661 taaaaaaaga tataacaaag aacacgtagc atttttactt gcaatcacta cgttgaaaca
     6721 agtattgact attcctgaaa tcaaagaagg gatattgttc caaggaaaaa cagtaggaat
     6781 tcgtgaagcc tataatctat tttgcgatga acaagaagca gctgtgtgga tggtcagcca
     6841 attagcacaa ggtaaatctc atccgcaaaa attcgaccag gcaacccctg tagaatatat
     6901 tgctgtaaga gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt
     6961 tctagaaacc gaatatttaa aagaggagaa aacaaatgaa aaacaataaa atcgcaattt
     7021 tagtagattc aggtacggat gttccagaag aattgataaa aaaatacgag atgtatatga
     7081 tcccattgaa aattatttat aaagatcagg tatatacaga taaaattgac attacccctg
     7141 aagagatcta tcaacgtttg ccagaggaaa tccctggtac atctttgccg gatggtgagt
     7201 cgatcacaga aatctttgag caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa
     7261 caatttccag tggattgagc gggacatata atgtggttcg tttaattgca caacagcaag
     7321 aagaattgga agcatatgta ctagatacga aaaatattgg tattggtgca ggtttttctg
     7381 cgatccaagc agcaaaatgg ctggaagaag gagtagaatg ggatcaactg atcagtaatc
     7441 tgaatgagtt agtcgaaaga acaaaagttt tctttaatgt tgcgacattg gaatatcttc
     7501 aaaaaggtgg acggatcggt ttagttgctt ctattgtagg aaccgcttta aagcttaacc
     7561 caatcatctc atgtaatgaa gaagggattt attacacagt aggaaaagct aggggacgta
     7621 aaaaaagttt ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct
     7681 tcaacttagc tgttgcccat ggggatgcga aagaagaagc aattgaaatg gaagcgagac
     7741 ttaaaagaga gttcccacaa gcaaaccaaa tctactttgg ccagatttct ccggcattag
     7801 tagtccacac tggtccggga ttattgggtg tcggtgtaca agttttagaa ggataaatca
     7861 aaaaaaggaa acactgaggt gaaaatcagt gttttccttt tttattgaag ttagaacaaa
     7921 aaataataaa gtattttgca ctttcatttt aattttctct tttgttttat aggcaaagat
     7981 gaaacaaaag cagaaatacg gtataatgag aaggtatgac aataatatat aagaggtggc
     8041 tctttatgaa aaaagtttta gttgtcgatg atgagaagcc gatttcggat atcgtcaaat
     8101 tcaatttagc caaagaaggc tatgacgttt atactgcata tgatggggaa gaagccttgg
     8161 aaaaagttgc tgaggtagaa cctgatttga ttttacttga tttgatgcta cctaaaatgg
     8221 acggcttaga agttgctcgt gaggtacgca aaacttatga tatgccgatc attatggtaa
     8281 cagccaaaga ttctgaaatt gataaagtcc taggcttaga gttaggcgcc gatgattacg
     8341 tgaccaagcc attttccaac cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg
     8401 gtgccacggc tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg attggtgatt
     8461 tgacgatcca tccagaagct tacatggtga cgaaacgagg agaaacaatc gagttaacac
     8521 atcgtgaatt cgaactactc ttttacttgg cgaagcacct tggacaagtg atgacaagag
     8581 aacatttatt acaaacggtt tggggttatg attattttgg agatgtacga acagtggatg
     8641 taactgtacg tcgtttgcgt gaaaagatcg aagataatcc aagccatcca aattatttag
     8701 tgacacgtcg tggtgtcggt tactatttgc gtaacccaga acaggagtaa ttggatgaaa
     8761 agaaaagtcc gcttttttcg atcagtcaat tttaagatcg ctattacctt tattctgatt
     8821 ttattgatct cgatcgaaat tattggtgcc tactttatac gtggtttaga acgttccacg
     8881 atcaatacat ttattaaaga tatgaatcaa acggtggaat cattagcaac aacaatcagc
     8941 ccggaattga atcgaaaaga taatgcagat gatgaagagg taaatgcgaa tatcaaaaga
     9001 tttattgaaa acagtgcgac tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg
     9061 atcatccgag gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat
     9121 gttgatatca atgatttcac gactaaacgt tatgttgctt tagataacga cgataaacgg
     9181 gtgaatatca atgttcaacc gatcctgtcc ccgaccggcg atgccgttat cggggctctt
     9241 tacgtcaaaa gtaatatcga acaaaaatat tcggaaatca ataacacagc agtcattttc
     9301 tttactgcct cactaattgc ggcatttatt tcaatgatcg tatctgtttt agttgctcgt
     9361 tcgattacac agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac
     9421 tacagtcgga aagtcaaagt gcatggacaa gatgaacttg gacagctggc tgataccttc
     9481 aatcagttag ctgaacgtat tgaagaagct caagatacaa tggaagctga acgcaatcgt
     9541 ttggatagcg tgctatccca tatgacagac ggcgtcattg cgacagatcg caggggtaaa
     9601 gtcattacga tcaatgatat ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc
     9661 ggacaatcga ttttgacact tttagatatc gaagaagaat atacgttgcg gaaattatta
     9721 gaagatccaa acgaaatggt acttgaccgt tcaaccagta cattggaaag tgatcagatg
     9781 atcttgcgag tagacttttc tatgatccgt cgcgaatcag gttttatcag cggattagta
     9841 gctgtcttgc atgatgtgac agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc
     9901 tctaatgttt cccatgaact tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca
     9961 ttgagcgaag gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa ggtaacgcta
    10021 gatgaaacag accgaatgat tcgaatgatc aatgacctct taaatctatc tcgcatggat
    10081 acaggtaata cccagcttca attagaatat gtcaatttca acgagttagt gaattttgtt
    10141 cttgaccggt ttgatatgat ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa
    10201 tttacgcaaa gagatctttg ggtcgaagta gatacagata aaatcatcca agtagtcgat
    10261 aatatcatga ataacgcgat caagtattca ccagatgggg gaacgattac ttgtcgctta
    10321 ttagaaacgc ataataacgt gattttaagt atcaccgatc aaggattggg gattcctaaa
    10381 aaagatctga accgagtgtt cgagcggttc tatcgagtag ataaagcacg tgcaagagca
    10441 caaggtggga caggacttgg attagctatt tctagagaag tgattaaggc gcatcgaggc
    10501 gctatttggg cagagagcaa ggaaggaaaa gggtcgactt tttatatctc gctgccatat
    10561 gaaccttatg aggaggaatg gtgggaatga gaatctctga aaaagtcgtt cgtataggac
    10621 tcattttgat ggttgcgtta agcatatact tctcttatgc aatctggctg agcccagcag
    10681 gaaaaagttc aatcaacgta gatgagacaa acactcaaat gatcgagtct caaagcaatc
    10741 gaaaaacttc cgaagtcttt cttccgcttc atgttacatg gcaccattcg ggaatgatcc
    10801 aagaaaccaa cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat
    10861 ttggaaagct ttctgaagta gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc
    10921 tgaactctgg ttttgagttg gcctataatg cgcctttttt gctaagagaa tacaaggaag
    10981 catttgattt agatttagat gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta
    11041 ctagaataca attcgatcaa ggaaataaaa aaatccgatt tttgaattat tcgaaccatc
    11101 tcgtatatga agcaaatatt tcgttgaatt ggacagatat caataaagaa ttaacagcaa
    11161 aagacacaaa atggacagaa atgacaacag aaccatcgac gatcgacacg caatatgcta
    11221 cgaaacattc gatcaaactg aagaagtata gttatatcct gtcacagcaa ccctatacac
    11281 tttttagaaa tgcttttttc cagaaaccag atagcgtgaa aaataatgat gaaagcaatg
    11341 aattgatttt ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg caagtagttg
    11401 attttcgtgg agagctgcca gagaaaaaag aaaatgctag tgtattttct gaaagtttcc
    11461 cctatattag caaattaggc agttctttag ggaacctcag atattttgac cgcagtgaca
    11521 atacgatcga ttatcggata ttcgtggaag gcttcccggt ttttgggaca gacagcagag
    11581 gacagattgg tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa
    11641 ccagtttgaa tacgattcaa gtgccgatac cttcagacga agaagtagaa cttgcaagta
    11701 cgaatgaaat catccagcag ttaaaagcta agggagcaga tagcaacaag attggttcag
    11761 tgatcgttgg ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac
    11821 ctgaatggta tgtgaaatat aaagataact ggtattcagc taacgacctt ctggaaaaat
    11881 tacctggatt ggaggcgaat taattggatt tcaaaaaaat cgaatggata tttttcgtcg
    11941 cttttttagg gttgaatgtg tttttattca gcagttatca tgacgcggta taccaagaaa
    12001 acaatgtcat tcgctctaat cagactgaat caatccaaca acgtctggct agcgacgata
    12061 tcacttacac ggggaagatt tcgtctgaga acaagcaagg ctattatctt agcgcggagc
    12121 agaccaatct tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg
    12181 caagtggtgt gtttttagac ggtgatgttt tgacgaaatc tttatccagt tcagatgctg
    12241 aaaagaacgt gatcgatcca gataaaatta gtagttcctt ggaagatttt ttatctaaca
    12301 aagacaatat tttattcggt aatgattatg tgtacacgaa aaacttttct catttagaag
    12361 aagaaccact agaaatccaa gcaagccaag agtatgaagg catcccattc aatgatgata
    12421 cagcaaaaat cgttctttct ttagaaaaaa acgataatga ttatagcatc acgaaataca
    12481 tgcagacgca tttatctgat atcgagcagc tgcgcgaaaa gacggaactg tatacggaag
    12541 aggaagcaat caatacattg tacatcaata acaaaatacc aagaggatca aaaattttat
    12601 ggcgtcagct agcttattcc tgtattctga aagtccggga aaaaaatgtg tatgttcctg
    12661 tttggcatgt agcgatcgaa acaagcgata aagtggtcca aattgagagc gtcaacgctt
    12721 tcagcaatac gatcgtgaca aacaatactg taccaaaagt ggaagatcaa taaaaaagcg
    12781 agtataatga aaaaagttta caaagtaaga agggaagtag gagtcaaatg gctgaagaaa
    12841 aaccagcttt taaaatcagt atcctagcaa gtggaagttc gggtaattca ctttatatcg
    12901 aaagtgaaaa aaaacggctt ttggtcgatg caggacttag cggtaaaaaa atcacttcct
    12961 tgatggctca aattgatcgg acaccggacc aattagatgg aatacttgtg acgcatgaac
    13021 atcgagatca tatacatggc gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg
    13081 ccaatcagca gacttgggaa gcgatggaac cactgattgg aaatgtacca gtggaacaaa
    13141 aacatctatt tgagatgggg aaagtcagaa cattcggcga tatcgatatt gagagtttcg
    13201 gcgtctccca tgacgcagcg gctccgcaat tttatcgttt ccataaagaa aatcggtcat
    13261 tcgtcatgtt gacggacacc ggatattgca gtgattatat gcgaggtgtc attgaaaatg
    13321 ccgatggtta tttgatggaa agtaatcatg atcttgagat gttacgcatg ggaccttatc
    13381 cgtggaattt aaaacagcgt attttaggag atagaggaca tctttccaac gaagacggtg
    13441 cacttgtcat gactgatgtg atcggtaatc ggaccaaacg aatctatctt ggccatctta
    13501 gcaaggaaaa caatatgaaa gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa
    13561 aagattttgg cgttggacat gattttgaaa tctatgatac agatccagat gaagcaactg
    13621 agctattttc gatttaaaca gttcaatcaa taaaaatata aaaagcagaa gataaaatca
    13681 atcagggttg gttttatctt ctatttttat ttttttagca tattaaaaaa ataaccgtat
    13741 tatcgactga ctacactgat gtgctgcttt tcatgtttct catccagtat ctcatctact
    13801 aatgcaatcg cgtaatctgc catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa
    13861 taatcatcac ttaacaaata agtacctgta cgtttgccat gtggatcaaa taaagaagct
    13921 ggactgatat aagtccaatg tagattttta gattgtttca attttaacaa tccttcgtac
    13981 atattgaaag ctgttggata gaagggtgca tttttaggta cttggtcaat catacgagtc
    14041 gtcttttctg tatctaagaa taacgaagag gcacccccaa cgacaattaa gcgtgtatcg
    14101 gttccttcaa gcatctcaat caaatgggac aaagaagttt gatgcatttc ttcttttcct
    14161 ttgggagcgt tgaaagcatc aacgacaaca tcaaaagatg caagatcagt agttgtcaac
    14221 tcaaataaat ccttttgtat aaacggaaca tctgatggca atttttctgg atgacgcacg
    14281 attgccgtca cgtctaaatc tctggacaat gcttcgtcta aaataaatga acctgcatgg
    14341 ccagtagctc caatgagtgc aattttcata aaaaacctcc ttttttattc ctgtccattg
    14401 taagcagttg ctgaatattc gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc
    14461 tttcatggtt cgtgatcatt gggtatagtt aaattaagaa aactgattga taaaaataga
    14521 agttgaagga gataagaaat gaaagagtgg ttagatcgtg cctttttacc aatgataaca
    14581 tctgttgtga atcttagacc cttaactgct ttgcgtaatg gaatgactta cacgatccca
    14641 ataattattg ttggctcgct gtttttctta tttgctcatt ttcctgtgga ttacatcgct
    14701 cagtggatag acaaaacagg attttctatg tattttacac aagtatacca ggctacattt
    14761 gatgtgatag ccttttggat cgtttttgcc attgcatacg cttatgcaaa agaagaagac
    14821 atcgatcgct taccagtcgg cataacagcc ttgtctgctt atctactgct tattcagcca
    14881 ctaaatactc agatagatac tgtctggaca ggtgctgaag ggatactagg agcagttaca
    14941 actgggctca tcatcggatg gggctatgca cgtttagtaa gacttgcgaa agggtggaag
    15001 caaagagaga agatacctga aaatgtttgg cagtctttta cttcattgat tccaatcatt
    15061 gttatagtca cttttacgct tatcggttca gcggtttttc aacatttgtt cagaatgact
    15121 gttgttcaag gagtatggac atatgtccag cttcccattc aatcgttcat agacacgttg
    15181 tttggtgtgc ttttattagg tttttttgtt ccgtttttat ggttatttgg tcttcatgga
    15241 tctgcgatgg tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt agcaaatgca
    15301 gaaatcttag cctcaggaca agagttgacg gtggcaaatg gcggacatat cgttactcag
    15361 caatttctcg atcagttcat gacggttacg ggtgctggat tgacgttagg ggctgtattt
    15421 tttatgacgg tttttgcgaa atcgaagaaa tacagagaat taggaaatct tgctctttta
    15481 ccagcctttt tcaacatcaa tgaatcaatt attttttcca caccgatcgt gatgaacccg
    15541 atgatggctg ttccttttat tttcgcaccg gtcgtttcag gattgattac ctattccgct
    15601 ttatattttg gattcgttcc tttatttaca gcagtccaag tcccttggac aacaccgccg
    15661 atcctctcgg gtttcttagt aggcggagtg cctgcagcga ttttacaagg tatcgtgttg
    15721 tcaattagtt ttttcattta tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa
    15781 aaggaaagac agacaaaaaa tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa
    15841 atactcctgt cgctttagat tgggaaaatc accaatctag gatgacagga gtatttatag
    15901 tcatgtttat actagccctt tttggacagc taattgaaca agaacaacta atgtgttcat
    15961 tcctacgtgt gtgatcatcg atgtccagat ccgaccagtt gctttgtact ctaggctgaa
    16021 gaagaaacct aggaaaaagt agattagcag atgaccatca ttatgagcaa acgcaaacag
    16081 caaagagctg atcactgctc cgacccaaaa atttgaatat cgactgatga tccccagtat
    16141 actgcggcgg aatacgaact cttccatgat gggtccagca acagttgtcg ccaaaataaa
    16201 agcgggtgct tccatgatca tctggatgat attttgtgta ttctcggaag gggttgcttt
    16261 gccaaacaat acagattcta acatgacagc gatgctttgc agaatcaatg cgacaaagat
    16321 tccaaccaat ccataaataa caatctttgg agcagaagct ttttttaccg ctgattcgat
    16381 cgctaagggt tcacgttgct taaaataaaa aatagctagg acaacggcgc ctagtatata
    16441 catgactgca gttgctgtga tcaattcaga cgtagatttt acaagtccta tcgaagctag
    16501 gatcaagggt gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc tatatttttt
    16561 taaagacatg taattcctcc ttttaaggga taacaagcat gcaaggcaag cttatatcga
    16621 ttgatttcaa ttatatcata atttcaataa gcagctgtca gaataaaatt tcttttcttt
    16681 gatttaaaag cttgcaaaaa agagggagaa tgagtattat taaaaatgta ggttagcact
    16741 cgaaggttaa gagtgctaac agattaaaaa aagagttatc ggagggattt atcgtgttaa
    16801 aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa actgtcggag
    16861 gaatcgtttt agcttctgct gctaaagaga aaccccaaac tgggacagtt gttgcagtcg
    16921 gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc
    16981 aagttatgtt cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa gaatatctga
    17041 tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat ttggaagata aaaaaataat
    17101 caaaaataat caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc
    17161 acgcgcagct atgttacgcg gggtagataa attagcagat acagtgaaag taacattagg
    17221 acctaaaggc cgaaatgtcg tactagaaaa atcttacggc tctccattga tcacaaatga
    17281 tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa
    17341 acttgtatca gaagtagcat caaaaacaaa tgatattgca ggagacggta caacgacagc
    17401 aactgttctg acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa
    17461 tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca
    17521 taatatctca actgttgttg attcaaaaga agccattgca caagtagcag ctgtttcttc
    17581 gggttctgat aaagttggac atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg
    17641 tgtcatcaca atcgaagaat caaaagggat cgaaacagaa ctagatgttg tagaaggtat
    17701 gcagtttgat cgtggttact tatcacaata tatggtaaca gataacgaca aaatggaagc
    17761 tgttctagaa aacccatata tcttgatcac agacaaaaaa atctctaaca tccaagatat
    17821 tctgccattg ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta ttgctgacga
    17881 tgtagatggt gaagcattgc caactcttgt attgaacaaa atccgtggaa cattcaacgt
    17941 agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc
    18001 tattttgaca ggtggtacag taattactga cgatctaggt ttagaattga aagatgcaac
    18061 aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat aaagacaata caacgatcgt
    18121 agaaggttct ggagaaaaag aagcaatcga agctcgtgtg caattgatca aaaaccaaat
    18181 tgctgaaaca acttctgatt ttgaccgcga aaaattacaa gaacgcttag ctaaattagc
    18241 aggtggtgta gctgttgtta aagttggtgc agctactgaa acagaattga aagaattaaa
    18301 attacgcatt gaagatgcat tgaatgctac acgtgccgca gtagaagaag gaatggtctc
    18361 aggtggtggt acagctctag tgaacgttat cagcaaagtt tctgctgtag aagcagaagg
    18421 agacgtggca acaggtatca agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat
    18481 cgctgaaaac gctggttatg aaggatcagt gattgttgat aaattgaaga acgtggaact
    18541 aggcactggt ttcaatgctg ctactggaga atgggtaaac atggttgaag caggtatcgt
    18601 agacccaact aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt
    18661 attaacgaca gaagcagtcg ttgcagataa accagaacca gcagcgccag cagctcctgc
    18721 aatggatcca tcaatgatgg gcggtatgat gtaagaaaaa gcaacttgct tatctacaac
    18781 tgatcattag tgaaagacag ataaaaagag gctggaacag aagcgtttaa ctccaagaaa
    18841 taagaagaaa ttcacggaaa ttgcttttca aatttttgtg aatttcagct tatttccgaa
    18901 ggagttgctt ctgcttccgc tgtttatacg
//
LOCUS       DAZKOK010000052        18563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000052 DAZKOK010000000
VERSION     DAZKOK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.052"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..898)
                     /locus_tag="ACG90W_001554"
     CDS             complement(<1..898)
                     /locus_tag="ACG90W_001554"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0477918.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSST"
     gene            complement(1275..1913)
                     /locus_tag="ACG90W_001555"
     CDS             complement(1275..1913)
                     /locus_tag="ACG90W_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477919.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1917..3899)
                     /locus_tag="ACG90W_001556"
                     /pseudo
     CDS             complement(1917..3899)
                     /locus_tag="ACG90W_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4079..4744
                     /locus_tag="ACG90W_001557"
     CDS             4079..4744
                     /locus_tag="ACG90W_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0477920.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5141..7174)
                     /gene="brxL"
                     /locus_tag="ACG90W_001558"
     CDS             complement(5141..7174)
                     /gene="brxL"
                     /locus_tag="ACG90W_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0477921.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7292..9814)
                     /gene="pglZ"
                     /locus_tag="ACG90W_001559"
     CDS             complement(7292..9814)
                     /gene="pglZ"
                     /locus_tag="ACG90W_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0477922.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9930..13562)
                     /gene="pglX"
                     /locus_tag="ACG90W_001560"
     CDS             complement(9930..13562)
                     /gene="pglX"
                     /locus_tag="ACG90W_001560"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0477923.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13626..17297)
                     /gene="brxC"
                     /locus_tag="ACG90W_001561"
     CDS             complement(13626..17297)
                     /gene="brxC"
                     /locus_tag="ACG90W_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0477924.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17322..17897)
                     /locus_tag="ACG90W_001562"
     CDS             complement(17322..17897)
                     /locus_tag="ACG90W_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0477925.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17897..18496)
                     /locus_tag="ACG90W_001563"
     CDS             complement(17897..18496)
                     /locus_tag="ACG90W_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0477926.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5719 a         3552 c         2606 g         6686 t
ORIGIN      
        1 ctgtactaga atctgaagtg aatgtactca aaacattttc aaagtctttg tctttgattt
       61 ttacattagc gtctttcaat acttcgccaa ttactttatt ctggaaggta ctgtcactta
      121 attttgtgtc tgttgcaatt tctttcaatt cttttttgta tttatccatg tcgttgcctt
      181 tgttttggtt tttcaccatt ttaaccacat aatattccgt agtataagtt gaagcattgg
      241 tagaagtgat cacatcagaa acttgaccat ctttcaactt aaaggctgct tctttgactt
      301 ctgccggaac cgttgtagta gtggaatcaa atttcacttt tccgccatct tcttttgtcg
      361 tagtatcagt tgacttgtct ttggctaatt tagagaaatc gtctccctta tcagcagctt
      421 tcttcacatc ttttgcatca tcttcacttg ctacttggat aatttgagct tccacttctg
      481 gatggaaaga ttcccaagcc gtttttaaat cttcatcggt caatttgata tgtttcttta
      541 gaccttcttg aaaggctaga ctttgtttga tactgtcttt gaatgtttgt tctgtgtaac
      601 ctgcagattt caattgtgaa tcaaatgaat cacccaattg tttctttgtt tgatcatatt
      661 gggaatcgat ttctttggtt gatacgtcat ctccatattt atcttcaaat acttgataaa
      721 cgatcaactg ttgcacagcg gaatctgctc cttgcatttt tttcaagcta tccattactt
      781 cttgttcgct gattgtaccg cctttataac taacggctgt tttttcaccg gcattcgtac
      841 agcctgacaa taagccagtg cctagtacga tggcaagtgc taaaaatctt tttttcattt
      901 atcaattctc ctacttttcg ttttttgcga attgctaaaa acaaaggtgt tcaagatgaa
      961 aatcatcaag aacaccttta ctaatctgct cgatacgtcc tgaatagata taaggctaca
     1021 caatccagaa agttcgctca gcagaataaa tgtcaagtag cacatcacac cttaatatac
     1081 tacttctttt gttaccgttt ttcaagtcta tcttgcgttc cgtatttaac gtaactaatt
     1141 cttagaacaa atgttatcga ttttgttttt cttcttccgc tgttgggata ttcaaatcag
     1201 acacttttat gttcgatttt ccagacattt ttttgttgtt tttattagaa aaagtttgtt
     1261 ttttcatttg ttttctaaag catgatcttc ttgtttccta ttttggtcag atatggatca
     1321 tgcgtgacca cgataactaa cgtaccttgg gaagcctgtt gcgataagat ttcaacgact
     1381 tgctccttat ttcgtttatc taatgatcca gttggttcat cggctaaaat gattttgcta
     1441 ttcttcaata agactctcgc acaggcgatt ctttgcttct caccaccaga taatgtgtag
     1501 ataggcgtac ttagcttctt gtttatattt acttttttca atgcctcata ctttgctgtt
     1561 cgcttatctg aaacatttaa attcgaaaac ttcaatccaa tatctaaatt aaaatcgatt
     1621 gtttcatatt caatcaaccc aaaatcttga aaaagaaaag agagggcatt tctttttatc
     1681 aagcgcttct ctcttccagt taaatgtttt gtttcttgat taaataaata aaattgccct
     1741 gaactgaatt gatcattcaa tcctaatata ttcaataaag tacttttccc tgctccgctc
     1801 tctccaacta cagtcacaaa atccccagca tccgcagtga aacgatccaa atgaagaatc
     1861 gttttgtttc gaatgatttt tttcgcacct tttatcacta tcaacggctc caattgttaa
     1921 cctccattca tcagcaataa ctgacttttc ttttctgtag acaaaataaa aacaactgaa
     1981 agaataaaat caatgaagct aaagaaaaaa atattaaaaa gatatttagg ctctctttct
     2041 ataagaaaaa ggaagattac acttgtcgtt ccccatttga tgaggttcat catgaagaaa
     2101 tctttatacg tttcaaagag cgaataacca cttgaacgaa agatagcgat tttccgacaa
     2161 ttgtagacaa aatatatgca taacatgaca tagctcaaca agaagaaaga aaagaaagtg
     2221 agtaaaaata tctgtatcaa gtaatgaaat gatctgctta acactttgac agctatctct
     2281 tcagtctgag tatacggaaa tgataactgc aagttatcac gtaactgact ttcttcaatc
     2341 agctcaaata acctttcttt cgacaaagag cctatcttga tctttaacgg tggatagatt
     2401 tctccgctga gaatatttct ttcccaatac gtactgtttt tcaatgtcag tatttcaaca
     2461 accggataat gttcgatcca ggggatagaa ggaataaatg tataaatggg ttgattgttt
     2521 tttataaata gaaatttcgg ttctttttca ctgatttctt gataatattc tgttatttgc
     2581 gccaggtcat tttttaaatc tgtatgtcta tatcgttcag ggatcaatat aacagggtgc
     2641 gtttcttttt cggttatttg gatcaacctt ccagagacat caatcaaagg aaatttctta
     2701 agataattca gatttatttt tatccctctg ccaaactgtt cattttcttc attcaagtaa
     2761 tctgatacat tgactaatat acttccgttt ttattcaaag tttcatataa ttcctgatcg
     2821 gctgctgatg caaaacgatt ggaataaata aaatccacgt ggtttttccc gatggttact
     2881 ggataaaata catgatagtc ctcaccagcg atttcttttg gtgtttcatg ggaaacagct
     2941 aactgtgaag aagaaaaaac gatagttgct aattcgacat tggcaacggt caaaagaaga
     3001 gaaaacaaca atccaaatgt aaaaagaatg tattggatat agttgaacgc ttctcttaac
     3061 gaagcgattt tttcaactaa taaaagagaa atcaaagaga taccctatat tccgctgaac
     3121 aagaaaacgt acatctggaa caaataaaga aattgctcct tataatacat aaaaataaaa
     3181 ccaccaatga ttcctgcaat tatgaatgta ataatgattt tctttttccc caataaacac
     3241 cacaatactt gatgtaatga aaagccatta ttcaacaata aactgatttc tttattcatc
     3301 aataagaaaa aggatgataa caaaaggaat aaaagaatca atccaatagt taaatacact
     3361 cgataattat atacgtctgt acttgaaata accgaatacg aatagctatt ttcaaagtct
     3421 tcaaaaatat tagtggcata atcagtgtga aatgcttgat tgaatcgttc cacaaatagt
     3481 aaaatgaacc gttcataggc ttctttttct ctcgtcatca agaaaaattc accttcaaaa
     3541 tcttttattt tgatcgattc ttcaatggac gttacgcgaa aggaggtatt agaatagggt
     3601 accttctcgt gagaagattt gacatcccct tgataataca atgtcatttc aagctttggc
     3661 tgtatcttga agttccaaga tgtatctact tcattggatt catttactgt ttttttcata
     3721 taagaaagct cagtttctga tgagacattt tcaaatacca tgattagctg ctcatattct
     3781 tcttgcccaa taaaatgttg tggcaatcga atcggagtat aagtatctgt ttgatggaaa
     3841 aattcatttg tccgatagag tacctgttta tttataataa ccaaggcaaa accactcatg
     3901 ataccggtca ctatgattgt gagtaaaact gtgaactttt ttttgattat caccactctc
     3961 ccttacttaa atgattcttt gataaaatca taccgttttt catcaaaaaa acgtatctat
     4021 caaatgtata ggtcatgtag aataaatcta tatactgtag aatcgaaagg agaagaagat
     4081 gaacatcgga gaaaaaatac gaacttaccg cagaaattgt aagcttacgc aaaaagaatt
     4141 aggcgaaaaa atcggtgttt ccgataaaac aatctctagt tgggaaaatt ctcgtacaat
     4201 gccagacctt gaaatgctgt cacttttaca tcagacttta ggtctgccga ttgattttcc
     4261 ttctattgaa tcagctaccg aatcaataga accaacattg ccggatgaac ctcttgttat
     4321 caagaaatct tattcatcaa aacaaccctt ctcactcaaa aagacactat ctttctttct
     4381 gctcgtcttt tgtttggtct atctttttct tatcaattat caatataaaa atgaaacggt
     4441 tgatcgattc aatccttttt tgaaaccaca aatcggctat accacactcc ctgtaggata
     4501 cgtggaaaat tccatcgaac attgggtatg taatgatatc tttgttcatg gtacatggct
     4561 gtcttttcac gctggagagg tagaaaaagg taagaatcat gctgtaatca aacacaaagg
     4621 aaaacacgtc atgaacgttc gcctgctcac ttgggaagcc attccctcag attaccaaga
     4681 aatcctcatc caagactatg acgatgcctt aaaaagaagc cctcaggagc cagataggca
     4741 ttaagtagga gatgaacgta gagatagaaa aaatcaatgc acatgatctt atcaaaaaaa
     4801 aacaggggta caaattaatt gaaaaagctt tgagatgaaa gttcctaaat aaaaaagttc
     4861 agaatgatag gtaaaaaaat gactaactat tctagtatta ggctatttgt attattcttc
     4921 tgttattttt atatcaaaaa atccagaaat aactaaatat aggtatttct ggcttatagt
     4981 ataatattta cgccacagcc cccaatgatt ctttttggaa tcatgatcaa ggacggacat
     5041 acacaaaaat attttgccca ttcgatccga atagattaac gactaatcta gtaagtaata
     5101 tagattggta cgtagtcaaa ccagcaaatg gaaaagttgt ctattccacc ccaagggctt
     5161 taaagacagc atcttccggc gtttgataaa aaatcaaatt aaaggtaccc attaattctg
     5221 ggggtacagt tccaatatca gcagcgctta tcataggtac aagtactttt ttcgctccgg
     5281 catcttttgc tacttgtaaa cagttagcta aattatctac cttaatcata gaaccaccaa
     5341 ttgtaatatc ccctaaaata actaaacttg gtaatacagg tttatttaag gaaactgagc
     5401 ataaagctat tatagttgat aacgataatg tttttgtcat accaatgcca ttcatatctt
     5461 gaacatgaac gacaaaatct tttgtcgtca aactaataga ctgactaaca cttccataat
     5521 tagctcttaa atatcggtat gccgaatcca tcgcttctct tgctccagca tctgaattga
     5581 tcccagtacg ttcaaacttt ccatttcccg aagtcatttg cgtttccaat ttataggtac
     5641 ctaattgccc agttttgcca taagataccg tgtacaaatg ccctggcttc cctaaccctt
     5701 ctggaatcaa agttcctcca ccttgttcag gaacagaaac atactcttct cgctcttctt
     5761 ctaaatcaat gtatgaaaaa ttgacatcat aaaattccat tccaccaatt ttctttaatt
     5821 gttcttttac acgacgacgc atttcaagag caaatcttag tatttcttcg acatcacttt
     5881 tttcaaatct accatcagga tacaacagtt tcatcatccc agatacaatc cgacgaactg
     5941 caatcgtatc ccgctgattt aattgagaac caagtttgaa atatttatca aaactatcgc
     6001 caaaactaac ttttcgaagc tcacgcatga cttcagaaaa ataatctgta atgaagccat
     6061 agtcatttgt aaaaaaatct ggtcgatatt taggaatttc ccaacctggt aaataacaat
     6121 gcatccgatc caaaaaggct gaatccgtcc ccataacctc cggaaatggc tcaaataaat
     6181 tcgaagtttt caatattaca tcaacgcttt gattaatatt tcctacaaaa gccattgatg
     6241 ctgaagcatt cttttcttct ttgcctctag caaaagatcc tgaagccatg taatctttca
     6301 tgatttgaac gccatcatta tccttaaatt taatcccagc aacttcgtca aaagcaacac
     6361 agtcccacat acctacaagt cccattgtac gactagccat attatagaaa aggtttgcta
     6421 ctgttgtttg tccacctgaa acaaggattg agtttggtga aatttcttta tatacatgcg
     6481 atttacctgt accacgtggt cctagttcac agagattaaa attattttca attaatggaa
     6541 taagtcttgc taaatgaagc cattttaccc ttttattaaa ttgtgtaggt tccataccaa
     6601 tggaccgtag aagtaagtca atccattcat ttgtagtaaa ctccttgcga gccatcttgt
     6661 aagcctcaat gtctaaacca ggcatttgga ttggatcaac cttttttgca ataaaagggc
     6721 tattccaacg atcattctca tcataccaat attccatcga aacaatacac cagatccccc
     6781 cacctaataa tcgatcaaat tttttcggat actcttcact aatttgaaca ttttttaatc
     6841 ccaggttaga gaattctgct tcataacaat ctttcttttc atttaaatgt acagttactt
     6901 tatcaataac tgtataacca cccaattcac gaatttttgc ttttactttt tctgcttcat
     6961 caggacgcac atagtttcga gcaagaatat ttttaacatt ttccacacct ttttcaacca
     7021 atgtttcatc caatgacgaa gcatacatac caagtaaata ttccaataca taaacaggta
     7081 cattcgcacc ttctttaatt ttctttgtta aatcttttct gacaatcttt ccgggaaaga
     7141 attcagataa ctttaaaatt tcctgttgtt ccactagaat tcctccatgt gttcagtcat
     7201 tataatagca attaaagtat atcatactta accagtaggc ttgataatac aaaagaaaaa
     7261 gtatccacaa aaaattgtgg atacctcgtt tttatatatc aaaatcaaat gattctgcaa
     7321 aaggattatc tataatgaat tttattcgct cggtcaaatc atttttttga ttttttatca
     7381 ctaaataata agtatcttca actgtatatc gacgatttac aaattcaaaa acaaatttag
     7441 taaaccgctg atttgcatct tctgtttttg aatccgcaac atagatttct tcatttgaga
     7501 tacgttcacc atattcatct tcaaaataaa gccagaaaga tgcggggtca taaacatcac
     7561 taattgcatt tttttgatag aattctaacg tggttgataa tcctgtaatt ctgcggttgc
     7621 tagtcattaa ttcaattgct actggttcct ttgcattgct acctttggct accgtaattt
     7681 ctagaacagg gactagcatt tcttgaatag atgaccctcc atgaacgtag ctttgtcccc
     7741 cgccagcttt cttaaaaata cttgaagtaa tcgggacaca aatgtttcta gtgtcatcat
     7801 ttcctaaaga atctcccaaa gagtaatttt tcgtaccaat tatttcgtat tcttcttcac
     7861 taagaataaa acgacgttct acacgatctt tttcatttct agatggattt tcaatcttat
     7921 ctacttcttc aacgtggctt cttttataaa taaatccatg atcagccgta actataaaac
     7981 gatagatact gccatcatta tgcaagcgat taataagtcg ggaaatttcg cttatcgcta
     8041 aatcacaggc ctcaaaaact cgattttcat tcgagtgatc acctgttcta tccacttgat
     8101 cgtgataaat ataaatgact gatttgccat taaatacttc tcgtacctca gccttattca
     8161 tcttaaccac atcatcataa tttaaagcta cagcatttgc atctcgttgc tggagaatcg
     8221 catttcgatt ggataaacca ttcgtttttt tcccatcaat cagtacttct gttccttcat
     8281 tgtaagttag ttgattgttt ggtaataagg cggctttacc gaattcagta actgaaggta
     8341 atacagaaag aaagctagcc atgtttgttg aatacttctt ttctctaact aacttacgtt
     8401 ctaaattcat ccctaactca tatcgaaaag catctgaaat aataactaca gttttaacag
     8461 agcgtttcat tacagcttga ttataaaatt ttcgaatgga tggacgattc ttaaattcaa
     8521 aagtttgatt ccaaatttgt cctgattgat caagaaactt cgaataattt gcttcaacta
     8581 atcgatacaa ttggccatat ttttgctttt cttctatatg atcactattc caaataaatt
     8641 tccgataatg cgtatcaacc caatacattt ccttctcata ttgagttagc aaattatcca
     8701 tgttcatcgg caagcacaat ctgtttttat ccacaagata ataagcatgt ttcaataatt
     8761 gatactcata aatataatcc gatgcgaaat gcattttttc gcgctgcttt aaactatcaa
     8821 aaattgtttg tcctgcaatt ttcgttgtag tgtcagaagc ctttaatcgt tctgcataaa
     8881 acattaatat ttttgcatga atctcagaaa atacgtctgc ttcaattaga tcatcaattt
     8941 ctatactgtc taataatcca tcaccatcaa tagcctgata tactttttta gatagctgtt
     9001 caaagctttc catgtaacgt gtatcattca taaactgatc catgaaagta ataatgtttg
     9061 ctttcaaatg tagtatttca aatcctttga acttcttcgg catctcttga tcaatttgat
     9121 aataaaaata gttcaaataa agacacatcg tcagcttttc caatgttggc tggctatccg
     9181 taaagccaaa tttcatttga ataaattccc aaaatttagc tgttaactga tacttatcaa
     9241 gttcccttaa aggattctcc ctactttctg taaagctttt tagaactaca cgcagtaaat
     9301 catttaatcg aatacttttc gatttcaaca aaacagccat tatcattaat tcaatcgttt
     9361 ctgatttatt aattgtcata tgcgtactaa attcaccaaa acttctcatg cgatcctttg
     9421 cattaaagaa attacgatac tgtttaactg tctgatgaaa tctaagtgga atagccaatt
     9481 cattcatgat aatcgctaag cggtctgcac taaattcctc tgaataattc aatacactca
     9541 acaaatgatt atcttcatct ttatcatccc aacgaggaaa tggcgcatag attaaatagt
     9601 tagtttcggg atcttcatat tccaataaac gcttagtttt aaattgtgcg tccttttcta
     9661 atcgatagat ttttgcattt tgaatgacca atgagtcaat ttcttcaaga aaatcctgat
     9721 ttgaatcaaa ccaaaaaatg attttcctac atccataatt aggaaaacgt tgatttaatt
     9781 tttcttcaat ttgttgcgta tttatatctg tcatcaatct cacctaatcg ttctctttct
     9841 atacccctta tattgacata atttggataa tttcgcacgc aatttaagaa taaaaaagaa
     9901 agttgttaga aaacaacttt cttagataac tatttttttg gttctttgac cccttttgca
     9961 aaaatttgga atttctcgcc ggttttagga tcacgctgca acttatcata attgacaatt
    10021 acaccatcat ctaagtctaa ttcaattcgt tttaatgcta agtgacccaa cttctcgtca
    10081 tactcacgaa cttcttttaa ttgcttctga atctttacaa tttcttttgt cattgcattt
    10141 ctttcttttg ggttatcact tgttgaaatt tgttgctgac gcaattcgat tcgattgtca
    10201 tacgcttttt gtagttcgtg taggtattca gtacgtactt ttcctaattg atctggtgta
    10261 tagcgatgca agtacattaa cgctttaaag ccattttgtt ttcctgagtc caattgccaa
    10321 taaattggtc gattggagta tgtttttaaa tggtctttgt aaaaatcttg tacgaagtaa
    10381 cgacgaataa tttctcgact tgtccctttg ccacctaaag tgtcagcgat aaactggaga
    10441 ttttcttcaa gggtttcttt accaaaagta atctcaacga aatcaatgaa acgattgaca
    10501 atgtcaattt ggctatcctt gaaatactca tcatcggtga tgagtaaaac attatcagta
    10561 tcaggtttaa atttttgata gcgggaagga tcccattcgc cgccagcgaa gatgagacca
    10621 tcttcgtcta gggagtaacg accgaagata caacctattg aataagaaat aaaatcctta
    10681 atttccctcg ccttatcagg taaccttgca gagatatatt catcttgaac ctcttttgtt
    10741 aattcttttt caagagaata atgattaatg aatatgtcgt tcagttcctc ttcattcgat
    10801 tttagagtat taaattgatt aaccaactgc ctacttagac agttaaatgc ctctgagaga
    10861 tcttttttaa acatataact taacaaaatg tttcttctaa actcgtatga cgtttcaaga
    10921 gcattatagt cttttctagc tatatcgata ttttcatcca ctttttctct tatttttttt
    10981 aattttctct cagatgtaac tttaagaaaa ggtagttttc ccattggtcc ttcatggtaa
    11041 tccaaggtgg gagaaataaa agttaaaaag tgattaacta atttagagtt tacatagcca
    11101 aatatttctt caaagtcagt tatatccttt gagaacagaa cagatccttt agtttcaaaa
    11161 attgcctgat tctgcaatct aaaagatact tgtccagatg aaagtgtcga ccaagtaatt
    11221 ccctttttaa aatagtaatt aagattttgt ggtctagatt ttaacttccc attttcatta
    11281 acataatttt ttatttcatc tccatcattc tcccagttaa ccacgtattc attgtttcca
    11341 taccattttc taaaacgacc acctttagtt gtaggaaacc atttttttct agacttttta
    11401 gcgtcctcta atgttttagc atcaaacttt attttgttaa attctacttc aaaccaatac
    11461 cgcaaaaatt tattgttatt tccggttgcc aacccttgtt ttgcatctgc aacatcgtta
    11521 gttaattttc cttgagcaaa atcagtgatt acgttatcgc ttgcccagta agcaatcggc
    11581 attcctggga tttttttgaa gttgtcttga ttagtactat aaaaataatc gcattcacca
    11641 tgctgatgaa ttgcttctaa cgttttaatt ttttgaactt ccattccacc tttaaaatct
    11701 gataatttta tatagcttcc atcttgttca tttgaatttt ttatcgtaaa cgtacaaatt
    11761 ggaacggttg cttcttcaaa agctgaatac tccatttgaa tcaaactaga aatgtttctt
    11821 tcattaatta aaaatgagcg taacttttca tagctagaaa tgaacatcca aacaaatggc
    11881 gtcataaagg aagcatatcc attatcaatc agcatttggc tattcaattt aataaacaca
    11941 gaaaatagat cttttttgac atcagaatag ttatcattta catatttttt taatttctta
    12001 tccattttat taagataagg cggattagtg actaccgcta catatttatc aactagcatt
    12061 tcagccacgt caagaatgtg caagtatttt tcttgtattt ccttcatccc ataaagaata
    12121 tccatattcg taaattcgtt gaattttgat atatactctc ttacttttcc taagtattct
    12181 ttagttattg cctgattatt tttatcgact aacttaatga ttgatccaag ttctttccca
    12241 ttttcaaatt gatcaaaaat taactctttg tattcattcc agcgttcact atacttatca
    12301 ttgttcaatt cactaatacg ctcttgatac tcgtctgtca ctttaggaga atcaataaac
    12361 tctcttaaat tcggcgatag actttttttc aaaaaaagac gatcgtattg acgccctttc
    12421 atcataattg caaaataaga caattgatac gctcttttat cgatttcaag accatataaa
    12481 ttgtattcaa cgattgcacg tgcagcatca cgctcagaat atcctaacgt aagataaatt
    12541 tccatcaata catcgaaggc gtaaactaaa atatgcccag accccattgc gttatcgata
    12601 atttttagtt cttctggttt taaattactt acagatgttg cttgttcaaa agcatattta
    12661 tctggaatta agtatttcaa cttatttctt aattcactat tgggataatg atctaaccaa
    12721 tactttccta aactattgtc aaccatataa cgaacgaccc aatctgtggt aaatagttga
    12781 gttgctgccg gaatatcttg ttttttgaca atccctttat aaatattcac cacttgatta
    12841 tgtggctcac tattatagta ctgatacaac cacccaataa tctctacctg tccaccagct
    12901 tgaacataga aatcatcctc tggaatactt aaaagatcag cgattacacc atctttttta
    12961 atataagaag ctgtaaacaa aagttctgta taatcttcta cttcttcaaa tagctcaggt
    13021 aagtaatcat tcaattgatt acagattttt ataaaaatca attgatacag cttgtccatt
    13081 gcttctgctg aattatctgt tttccaagtt ctgacttgtt gctgttcatt actagataat
    13141 tcttcaaaga tatcggcatc ataaatatct gtaataatat ctggctcaat tttcccactt
    13201 atttcactag ataatacacg atacgtgtca ggtaaataat catttacctc cataaaccga
    13261 atggcaatca atcggttaaa ccacgtataa gcaacctctt cgattaactc ttgaaaagct
    13321 tctgaataag aagtgacaga agcacgttgt tttagttctg caatcaattt gtttctttgc
    13381 gctatttctt ttccttgaat tcttattcct gatttttcga tttcttgtaa attgctatca
    13441 attttcttca catcttcaat ttttgattca tcaattccta aacgttttgc tttagtcatt
    13501 acttcatcaa ttaattttct acgggcattg accgcgaaat tctttatcgc tttcttatcc
    13561 atttttcttt cccttctata tgtaaccttt gaaaagacag gcaccttttt atgcctgtct
    13621 tttcactaga attgaatcgt gatttctgta ttttcttgaa gttgttcctt cagtttttga
    13681 ttaatttcag ctgttaactc atcaatatct gctgctgact taatagtttt aacatgaacg
    13741 ttaagtgttc tcattggtac tgttttctgt tgaatagtag gttttactat cgctatttct
    13801 tcagcttttg ctgcagagtc tatttcatct ttttcagttt cacgaatcaa gtttgctgct
    13861 tcaatttgtt tacgtgcttg ttcttcttga cgtctgcggt tttcttcatt atcgaaacgt
    13921 tgattaaaga tcaaatactg ttctgctacg tttaatgata agaaaacaag atcatctata
    13981 gtattcgctt gttcaatttt tgctttcagt tcttgaaatt cttggtcaac tattggtaca
    14041 aattgatcct taaaatcttt tccttctaat aatttaaatg ttgcttgttt ttcttccgtt
    14101 aatttattta atatttcctg actttccaac tctaacagtt gattatattg ttcaataaat
    14161 tcttgaatca actcatttag tttcatcata tctgaataag gatcagcctt tttcaaaatg
    14221 gttttcatct catgaatcaa ttgatcaatt tcttggctag aagtttgacg ccgacttttt
    14281 tcgattaatc taattttatc ttgtgcattc tcccagattt ttttctgagt actattttgc
    14341 ttgtggaaat ttttaatttt atcatatgtt tcagcccagt cgaataattc atcgcgatat
    14401 tttttgattg tataaaaaaa gtcttcaaca tctgacgttt gaacaattac ttcaagatat
    14461 ttttttattt gctcatattc ataagcacta gggtaggtat aagtgttttg taaatcaaaa
    14521 taagagcgta aattgtctaa ttgacctttt aaatatttct tgaaattttc cattgtatgt
    14581 tcattgttat tttcatcaac aatattacga ctttgaaaca gttctctacc gatttcgtca
    14641 accaattttt ttgcttttgg cgaaatcacc gcgcttggtt caatttttat cttttcactt
    14701 tctcgtcgtt tttgaataat cattacaata ctatttggat catccatccg tgaaatttgt
    14761 acattgttat aaagtaaatc aattttacca gacatgaaaa gtttagcgat gcaccattcc
    14821 acatcaagtt ctgtgaatcc atacggttgt tttgtttcaa aacgatcacg aattgttttc
    14881 atcgtaatcg ttaggtgatt tctcttttga tcattgataa aattgaaaat ttcctgaact
    14941 gcttgctcat ttgatggatc ttcaataatt tcatttttgc tttctttcac taaatataag
    15001 atatcttctt tttctttagg cgcatcaatt tggtttaatt tatggtatac ttcttcagcc
    15061 attttctgaa gtgctgattg gaattttgct ttaaagtcgg agcctgtcag ttgaatttct
    15121 gcaccgttat agaaaaattt tgcagatttc aacagttcat ttaattgatc gttgatactt
    15181 gcacttagtt cctgtaattc accagatttt tccgaacgaa tcgcgtcaaa gttctttact
    15241 acactcgaat aagtcgttgt taaataaaga ttcattctag catacattgc tatatcatct
    15301 ttaaattctt tatctgatgg caattctacg aataataagt tacgggaact cttaagcgca
    15361 agttctgcct cttgacctgc taaatcagag tatggtgaaa taaaattaac tgtcatttca
    15421 gcatttccag cttgcttgta tggacgttga tctaacactt gtgcataagt aaacgtatac
    15481 cggtttttaa atttatttcc acctgacatg cctaattttt cataggcaaa actatttcgg
    15541 tctagaacgc cgtcaaaaat tcgtttagat agttctacga tcaaatcact atctgtaata
    15601 ttttgatttt taatctcacg agtaatttcc tgttcttcat cagtttggaa aatataacta
    15661 tctaagttct tccgcacaaa gttttcctta attaaaaggt ccaatgcttc ttctactcgt
    15721 tttttagttt ccaggcggtc ttcgttgata tttcttatca tcaagctcgt aatattttca
    15781 acatttggtt tgatggcttg atcaacatat ttaatcatga ataaaattcg taagacattt
    15841 acgttaaagt tatctatatc agttggtctt tgaatatgag aattatttgt agcttgtgta
    15901 atcactcggc taattgctgt atcaatccaa cgttctaacg agtaataaaa ctgatctaaa
    15961 gaaaccaaac tggttgtatc attttcctta tttcgttcag cggcttcttt aaacattgct
    16021 agtaatgaac gttcaccaga cgataagttt gatccttgat agctgtgatt acgaatctcg
    16081 gttagaattt gccccaataa attgaattga tagggaataa atggataaac ttctgcgtaa
    16141 ttttcagcat cttggaagag ctttaattca ggactatcaa caaagagtaa tttatttttc
    16201 aattctgttt gaacagtttc aaattgtaag gctaaagaat cattggcaaa ctcttttttc
    16261 ttcaaaatac gttctttaat tacttcatcg gcatttgctg aagataaact cacagttgtg
    16321 gcaaaacgat ccttgatctt agaaaaatca ttatcagaat tgttatcaaa gtttgttacg
    16381 tgatcaatcg cttcctgact tgtcactaat acccaagcct taccttcaca aattcgaccc
    16441 aaatcttcca caacggtttg taactgcaaa gtcaactcac gattaccgcc gatatattgt
    16501 ccgacctcat cgactagaaa ggcaatatga tggtctgctt ctttggaatc aagatagctt
    16561 ttaactagat ttgcaaagtc atcaatgctt attaaataat ttcctgatgc tttttctaaa
    16621 aaattatcaa tagcggcttc actcataaaa tcgattttaa gtgccgcttc tttgatatca
    16681 tcttggatcc aatcaaactc atggcgctct tcttgccatt ttgccccatt aatctcttca
    16741 aaagcagctt tgaatgcatc aaacttttct tctttaatta accgacgttc aaaatctgct
    16801 agatgtggaa tcgtgcttaa tccttgcatt tcgttaaata cctttaaaaa aacattcacg
    16861 attccgtttt tgtctttttt accacctgca tcactttttg aatcaatatt aaacagaata
    16921 acatctgtat ttatttgtga gcaccgttcc atcgttgcca aagtcagtgg atctttgatt
    16981 ttcccatctt ctttgaagta atcaatggct ttcttgtcat ttatttctcg atttgcgagc
    17041 aagtaagaaa taatttttaa gaaatgagat ttacctgaac caaagaaccc ctgaatccaa
    17101 acacccattt tatctgttgg tgtattgata ctccgttggt aggcatcaaa aattgtttta
    17161 aagtgttttt gtagttcttt cgtaaccacg tattcttcta attcttgttg aacattttca
    17221 tcctttgctt gaccgacctt aataactcct tgaatatttc ggtcaattgg ttttgcaaaa
    17281 atatctttaa ttagcattta atctcctacc ttccatccca actaaattct aaatgctcga
    17341 taataattat catctttatt ggtattaaga attgtcaaac ttaattcatc atattcccct
    17401 ggatacatca tcacaactgg tacgcgatca aatacctgat gcattttatt taaaatagag
    17461 tgcgctctaa ttaatggata taattcgcca attcctgtca ggaacacaac tgatttttct
    17521 ggtgtatggg catccacata ttcaaaaaat acattttctt cctcaggtaa atccattttc
    17581 aataaacgac caatgctttc cactaatttt tgtaaaccac gtcgttcttc catcttaatc
    17641 gctttttcag tgaaaccgcg tttttctaga tactctaaca taatcacata gagattataa
    17701 actttcactt cataattatc accaacagat gttccagata tatcattaat ataatctctt
    17761 acaattaact cgtctttcgg atcgtagtca aagatataat agcctacttc attacctaaa
    17821 ccacgattca ataaaaaatc ttctttttga attacttcat tcaacttttc caaatgctct
    17881 tgaatcgata atgacattat aaaattcctc ccagtgctag tacaatatct ttacgattct
    17941 tgtcaactaa ccattgaatc aatcgtttat ctaataatat cggagtcatt cgtccatatt
    18001 ctttttcccc tctgactaat cctgtttcaa ttagattcgt ttgataagcg gcaataagcc
    18061 gtttaatcgt ataatctttc cattttgaaa tatcttctga acttctccgc ttctctgtaa
    18121 aaaaagaacg gtaatcaact ggtgttaatt gcaaatttcc tttacgtaga ttttcagcat
    18181 atacctcagt cataaattca ttaaaaagat catcaatcat aaggatggaa atcaaattca
    18241 ctaacttttg attatctata tttaactgat tgaaaagttg caatccagct tcatccagtt
    18301 gactaactct ccttttcacg ttctgataga tttctttttg acggttactg ctcactgcaa
    18361 aaaaaatatt ttcatgttca tttttctctt taatttctat atctgtatat ccttctagtc
    18421 gtaattgaat atataacttg aactctgaaa accaaaagcg tttagaaact agaccagcag
    18481 aatatttttt attcaataga ttgcctccta atcatctata attcaataag taaacaagtg
    18541 ttttttgctc atcgttagca ttc
//
LOCUS       DAZKOK010000053        18504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000053 DAZKOK010000000
VERSION     DAZKOK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.053"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG90W_001564"
     CDS             99..746
                     /locus_tag="ACG90W_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0477927.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG90W_001565"
     CDS             1139..3784
                     /locus_tag="ACG90W_001565"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0477928.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG90W_001566"
     CDS             4094..5407
                     /locus_tag="ACG90W_001566"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0477929.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG90W_001567"
     CDS             5397..6050
                     /locus_tag="ACG90W_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0477930.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG90W_001568"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG90W_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0477931.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG90W_001569"
     CDS             6947..7147
                     /locus_tag="ACG90W_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0477932.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG90W_001570"
     CDS             complement(7401..9080)
                     /locus_tag="ACG90W_001570"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0477933.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG90W_001571"
     CDS             complement(9082..9294)
                     /locus_tag="ACG90W_001571"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0477934.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG90W_001572"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG90W_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0477935.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG90W_001573"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG90W_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0477936.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG90W_001574"
     CDS             13269..13466
                     /locus_tag="ACG90W_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477937.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG90W_001575"
     CDS             13619..14104
                     /locus_tag="ACG90W_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0477938.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG90W_001576"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG90W_001576"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0477939.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG90W_001577"
     CDS             14981..15508
                     /locus_tag="ACG90W_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477940.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG90W_001578"
     CDS             15599..16336
                     /locus_tag="ACG90W_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0477941.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG90W_001579"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG90W_001579"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0477942.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG90W_001580"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG90W_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0477943.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZKOK010000054        18453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000054 DAZKOK010000000
VERSION     DAZKOK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.054"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..185
                     /locus_tag="ACG90W_001581"
     CDS             18..185
                     /locus_tag="ACG90W_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0477944.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(247..2058)
                     /locus_tag="ACG90W_001582"
     CDS             complement(247..2058)
                     /locus_tag="ACG90W_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0477945.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2055..2420)
                     /locus_tag="ACG90W_001583"
     CDS             complement(2055..2420)
                     /locus_tag="ACG90W_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0477946.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2583..2978)
                     /locus_tag="ACG90W_001584"
     CDS             complement(2583..2978)
                     /locus_tag="ACG90W_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0477947.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2996..3958)
                     /locus_tag="ACG90W_001585"
     CDS             complement(2996..3958)
                     /locus_tag="ACG90W_001585"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0477948.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3958..4170)
                     /locus_tag="ACG90W_001586"
     CDS             complement(3958..4170)
                     /locus_tag="ACG90W_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0477949.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4191..4889)
                     /locus_tag="ACG90W_001587"
     CDS             complement(4191..4889)
                     /locus_tag="ACG90W_001587"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0477950.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4909..5451)
                     /locus_tag="ACG90W_001588"
     CDS             complement(4909..5451)
                     /locus_tag="ACG90W_001588"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0477951.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5583..6587)
                     /locus_tag="ACG90W_001589"
     CDS             complement(5583..6587)
                     /locus_tag="ACG90W_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0477952.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6584..7573)
                     /locus_tag="ACG90W_001590"
     CDS             complement(6584..7573)
                     /locus_tag="ACG90W_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0477953.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7570..8376)
                     /locus_tag="ACG90W_001591"
     CDS             complement(7570..8376)
                     /locus_tag="ACG90W_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477954.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8542..9498
                     /locus_tag="ACG90W_001592"
     CDS             8542..9498
                     /locus_tag="ACG90W_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0477955.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9575..10093)
                     /locus_tag="ACG90W_001593"
     CDS             complement(9575..10093)
                     /locus_tag="ACG90W_001593"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0477956.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10181..10330)
                     /gene="rpmG"
                     /locus_tag="ACG90W_001594"
     CDS             complement(10181..10330)
                     /gene="rpmG"
                     /locus_tag="ACG90W_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0477957.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10558..11004
                     /locus_tag="ACG90W_001595"
     CDS             10558..11004
                     /locus_tag="ACG90W_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0477958.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11199..13094)
                     /gene="asnB"
                     /locus_tag="ACG90W_001596"
     CDS             complement(11199..13094)
                     /gene="asnB"
                     /locus_tag="ACG90W_001596"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0477959.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13419..14393)
                     /gene="bsh"
                     /locus_tag="ACG90W_001597"
     CDS             complement(13419..14393)
                     /gene="bsh"
                     /locus_tag="ACG90W_001597"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0477960.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14727..14814)
                     /locus_tag="ACG90W_001598"
     tRNA            complement(14727..14814)
                     /locus_tag="ACG90W_001598"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14780..14782),aa:Ser,seq:cga)
     gene            complement(14959..15525)
                     /locus_tag="ACG90W_001599"
     CDS             complement(14959..15525)
                     /locus_tag="ACG90W_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0477961.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            15781..17111
                     /locus_tag="ACG90W_001600"
                     /pseudo
     CDS             15781..17111
                     /locus_tag="ACG90W_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            17077..17427
                     /locus_tag="ACG90W_001601"
     CDS             17077..17427
                     /locus_tag="ACG90W_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477962.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(17519..17938)
                     /locus_tag="ACG90W_001602"
                     /pseudo
     CDS             complement(17519..17938)
                     /locus_tag="ACG90W_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(17939..18283)
                     /locus_tag="ACG90W_001603"
     CDS             complement(17939..18283)
                     /locus_tag="ACG90W_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0477963.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
BASE COUNT         5589 a         3811 c         3125 g         5928 t
ORIGIN      
        1 taaatgaggt ggattcaatg aaaaaatttg tatctggaat attagtagga agtctggcaa
       61 cagccgctgc cgttgctggt ttggttgctt ctgtgaaaaa aacagtgatc gatccaattg
      121 acgaaaaaga agcaatgatc gaagaaaacc gtaaaaaagc aatgcgtaaa cgcgtttctc
      181 gttaataatt atttaaaaat acgcatgaag actttctaaa aaacagataa gtcatcatgc
      241 gtatttttat ttgtcagtca atataagttg tgctatttta tctgcctgtc tcagattcca
      301 tggatctgta taaggtctag ctactgctgc agttagccaa tccacacctt caacaggtat
      361 tccatagcaa taaaagtcag actggatttc ccctgaagca tcaatcacgc gattcgtacc
      421 tcgctcaaca tctatagctt ctgaatgata acttcctgta ttatctgaaa atgaaaaagg
      481 gcgaacaagt ttgttttcct tcaaattttt tatgacagga ttacttgaac gagaaaagct
      541 tgtttgtggc aatcgagctt caatcaaata gcgactgctg atttctttat cttgtagaga
      601 aataacatag cggttgtgct tttgctgaat atcaaatggc ggttcgatca aatgaaaaat
      661 acccgcatct atcagtgctg ctaattcttt cgtccgaatg attggcggac cgatcgttaa
      721 aaagctattt agatgagtaa accatcccca tagtatttct ttgtattcct tagcagaaaa
      781 aacgtgccat tcaatagcct gccggattgg atcacgccag tcttttaatg catcaaatgt
      841 tgaagcaaat gctcctgtgc aattgccttt ttccgcttct tttatctgcc cttcaataaa
      901 tattcggcta gcttctgaaa agggctcatt ctgataggca agaggagttt ctaaatacga
      961 ccaatcccat ctataaggga ttagtgctgg atgctttccg agacactcct ctgaagagag
     1021 ggagagaaaa tcatgctcaa aatttttctt tggaatcggc aagtgatatt cttcgatgat
     1081 tttcttgtaa taaaacaact caacgtcttt tttcagatag tcaaaaaatg tttcgccagt
     1141 caaaaatccc gactgatgcc attgctccag gttttcattt gttagtaacc gtggccatgc
     1201 cattgctccg ccttgctttt gatttcttcc tcgagggtaa taagggagcc cccttcttga
     1261 tccactgtat acaatcggtt ccttgcctga tggatgatag accagttttc ccttttcgtt
     1321 ctcgaagcgc cctcctcttg atactgtaaa caatccgaca taatcaaaaa aacttaagcc
     1381 tagacctctt aaaaagacat tttctttagg cggtatcttc gaaagatcta catctgcagg
     1441 attagaagga agctgataca gtaaaacatt tttctttgcg tagtctaccc acactctttc
     1501 ttcttctgtc tcatcatttt cccagtgacc gcttgctaaa acaagctgat cgatagaata
     1561 gctttgctga tctgtctgta aaagataacc atcttttctt ttgcttactt tttttactaa
     1621 agaataaaca aactgaatag aagctggaaa ttcttcttgc aactttgaga aaaaccagcg
     1681 ttgatagagt ccgtataaac atcgagtaga ttgctgatct ttttccagac gattgatttc
     1741 ttctataatt agttgcttat ttttggttac cgtctgttcg atataatctg tagcaggtcc
     1801 tttactccat tgaaacaagt tgggtccctg tgttaccaat cctccactgg acaatgtttc
     1861 atctgtaaaa agcgtcactt gttgagaaac agaattcatt aataactccg gtggctgatt
     1921 caaccgccaa actcgacctc caggaccatt gggatcgaaa agaaaaagtt caagagaatg
     1981 tctgtcctga ttattatgga gaatcctttc tgctacactt aatcctctag gtccggcacc
     2041 aatgatccct attttcattt gcatgcctcc atttcgataa attttttcaa taaaacagca
     2101 tggttctgat gttcattttt tgcaccataa acaaaagtaa tgactggatg ttctttcata
     2161 agttgactga gtttaaaaat agcttgcttc ttttcttctt cgaaaatcaa ttcttgctgg
     2221 tatttctttg caaatgaagc aaatcgttct ggtacatggt caaaagtttt gcgtgcttca
     2281 ttactaggtg caatttcttt caaccacaga tctaactgcg ctttttcttt tgaaattcct
     2341 cgtggccaga atctatccac gagtattcta tagccatcct ctttttcagc atcaaggtaa
     2401 acacgtttta atttgatcat agttgtttcc ttctttctac cttttcgctt agcttatata
     2461 tgtagataat agcacaaaat aaaaaaaatg aacggcacaa ctatccattg cgccgttcat
     2521 gtgatcttgg tcaaaatcta atgattgatt cggtttttaa agctgtcata caatcatctg
     2581 ttttatactt ttttactttt gatttttcca gaccatccgt catatccacc cttaagtaaa
     2641 taaatctctt gataacctgc tttacgtaat ttatttgctg tacggatgct caaacttttc
     2701 ttttgatcgt aaatatagac aggctggtct tttcgaatag atcctaatga atctttcaaa
     2761 acagtgtaag ggatgtttct tgcacctaaa atatgcccag catcaaacac atctttttcg
     2821 cgaacatcga ttacttgtgc ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc
     2881 gttgctgaac gtttggccat tactcgtaga tagacttccc aaaggatgat tgcaagcaca
     2941 atcaatagca aaatgatatt aattacccat aaaaccatgt aatgcctccg tttttttaag
     3001 caaatttcaa ggctaatgag gcagcaccaa tgacaccagc ttcattacca agctctgcta
     3061 atttgatttt tgttgattct cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta
     3121 cacggctacg taaaaattct cctgctgccg atactcctcc acctaacacg atacttgacg
     3181 gatttaatgt attccccaag tttccgcaag ctaaacctaa gtagaagcat acgcgatcaa
     3241 caaccatcaa tgcaaacgga tcgtcttctt gagccatttc aaagatatct ttacttgtta
     3301 cttcttctcc gttgtctagc attgctttca gttttgaatc ccctgcgtat tcttctgcca
     3361 agaaacgacc taggcgtacc acacctgtag cgctagatac agtttctaaa catccgcgtt
     3421 taccgcaagt acattcgaac ccttcaggat ccacagtgat atgtccgatt tctcctccag
     3481 ctccgctcac gccgtgaatc aaatttccgc cagcaacgat acctccgcca acacctgttc
     3541 ctagagtaac gaatacaacg tctggatcgt tttctccagc acctttccat cgttcaccta
     3601 aagcagcaac attcgcgtca ttatcgatag cgaatttgat tcctgttcct gcctcgatca
     3661 gttttttcac aaattgaact tctgtccagt ttaagttata cgcaccaata accgttccag
     3721 cttcgctgtc cacgcttcct ggggtaccca tgccgattcc gataaagtct tcggctttca
     3781 tattatataa attcaaacga tgattgattg attcaatgat ttcaggaacg atatgcttac
     3841 catcatccaa aatattggta tcgatgctcc atttttgctg aatctctcct tctggtgtta
     3901 aaattgcaaa ttttgctgtt gttccaccta aatcaatccc aataatcttc ttgtccatta
     3961 caaatttcct ttctttcggc tttcttcaat tcggtgctct ctttttaata tgcgccacgc
     4021 tgtcatgtat gtatcatgat caatcaaacg gctatcatac agattacgca attcgatagt
     4081 cattaattca atgtcataga tgcgtgcacc cacatagata aagatgccaa attttttgaa
     4141 cagctgctgt acatcatata agctctccat ctttaagcac ttccttcata ttatacaagt
     4201 aaaccatatt tttttaaacc cagtaaaata catattacaa aaagaaatac aaaaactagt
     4261 gtagaaagaa tcctttggtg tatcgaatag cttgtctgtt gtttcggtag acccaaaaca
     4321 ttccctaaca ctaatccgcc aattagccca ccgacatgtc cccaaacatc tacagaggta
     4381 tccagaagac caaaaacaaa tgtcaacaca ataaataaaa gaaaatgtct gactagctgc
     4441 tggatcgctg ggttatgttt aaaatgaaac cctaagataa acaacgcacc gaacaaaccg
     4501 aatatcgaag tacttgcacc accagacaga acattcgctt cgttaaaagc aaagcttgct
     4561 gtatttccca ttatcccgct gaacatataa atcaagaaaa aacgccaatg gccatagatt
     4621 gcctcgattt gctgccccat aaaatacagt atcagtgagt tcattacgaa atgcatcagt
     4681 ccaaaatgga tgaagattgg tgtcactaaa cgccaccatt cattgaagtg aacaagataa
     4741 ggaccataca ttccggcata ataagggatc tcaagtcgcg gaaataattc catcatgagg
     4801 aagaccgctg tctgtatccc taaaaaagca taggtccata acggtcgtct taaccataca
     4861 cgcaatttta tttgctgttg ataattcatg aaaacctccc atttcatttt agtcaataaa
     4921 caattgttgg atcttctgat caaagctctc cggttgccat tgttcattca attgttcacg
     4981 aaaaacaagg ctacatgttt ttttatgaaa attatttaaa taccggtcgt aataaccacc
     5041 gccaaaaccg attcgataac cggctctatt gtagataata cctggtacaa tgaggagatc
     5101 gatcatctcc ggcaaataga gcttctctga ttgtggctca agaacaccaa aactcgaacg
     5161 tacaagaacg gactgttcat ccatttcata aaacgccatc tgacgtttcg gcaaagtttt
     5221 aggtacaact attttctttt gttctttttc tgctcgttgg ataaccggct gtgtatccag
     5281 ttcaaattca ttcgataaag tcgtccctat cacttttgct gacttccagt actcgctggc
     5341 gaatagctgt ttatagattc gctgttcttt ttcctttttc ttgccacttt tggccagatt
     5401 tttcagcttt tcgattcctt tccggcgtaa tcttactttc aattggtcca aaaactcacc
     5461 cttccttctc tcataaaatg gtataggaaa aaaagaaaaa tagcaatcga actttcaaat
     5521 aaatgcataa atagaataaa aagaagacct agaaatgatt ggccttcctt ttttaatgaa
     5581 tttcaactgt tcagcgcgtg tttttgtctg ataaaaagtg catacagaaa atatggcgca
     5641 ccaaaaaggg caatcaaaat tcctgtaggt acttccccac ttagtagaat cactctagca
     5701 accatatctg cactaagcag aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag
     5761 ttattttctt tacgtacgac tagcttcgca gcatgaggag ctagtagtcc tacaaaagat
     5821 aaactacctg tcaccgctac actaccgctt gccaaagcaa cagctaacaa caaaaggatt
     5881 ttttgacttt tagccacgtt aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc
     5941 aatacaatcc tctgtgcata aagtcctcct agcggaatta agataaccgc ccatggaagc
     6001 aatgcccaaa cataaggcca gctcgcaccc cagattgatc ccgctaacca tgcattcaca
     6061 aatccataat tttccttaga taattggatc gtgcatagca atgttaatgc agaaaggccg
     6121 gcattaacgg caataccgga caataagatt cgattcatac ccaaaaagcc atttcgatca
     6181 taggccgcaa gatagacaca gcaagcagct aaaacaccac ctagaaaggc tacaactagc
     6241 aatagaaacg gcagcgaata gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct
     6301 gctccggcat taatgcccaa tatactagaa tctgccaatt catttcttgt tactccttgc
     6361 aagatataac cagaaacgcc aagacaagct cctgctaata aactgatcag taatcgagga
     6421 actcgaaatt ctctaacaat cagcgccaaa gaagatgatt ctttccctat gaacgtggca
     6481 aaaagctgat ccaggtaaat aaaaggcgta ccaatcatta aactacctat agacaggatg
     6541 acaagtacaa aaagcaagaa atagaacgtc caatatattt ttttcaccgt tgttccctcc
     6601 ttacttgtat cagtaaaaac ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt
     6661 caaaaggggg attgatcatc ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa
     6721 ctaaaaaaga gacaggtaga acataacgat agtccgttcc aaccattccg cgaacaactg
     6781 gcggaaccaa cagcccaata aatgaaacag gtccgactaa cgaaacggca atggctgcta
     6841 acactgcaac tagacagaaa acaatcaacc gcgttcgttt aggatgactt ccaagagcta
     6901 atgcatgtgt ttcactcaaa tgaatcaaag atattgcttt tcctaatgaa aaagaaacca
     6961 aaacagcaag tatatataaa ggaaacaata ctgctaattg tttccatgtg atattggcag
     7021 ctccgcctac aaaccaaaaa gcaagatcct gctgcagatt aaagaattgg gtaagtgctt
     7081 ggctgatagc agtaaaaaaa gaactaatag ctacgccagc taaaatcagc tgtaaaggat
     7141 tcatgcccat tttcgaaaac tgcaaaaaac caaatacgag caaagttaca gcaaaagaac
     7201 ctgccaatga cattagaaac acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa
     7261 gggccattgc caacgaagca cccgcgttga ttcctagtaa gcctgaatct gctaaaggat
     7321 ttcttgtcac tccttgcatc aatgcaccgc tcaatgcaaa acaactccct acaagaaatg
     7381 ctcccgccac acgaggcaaa cgaatatttc gtatgatttg gtgactttgt tttgatctat
     7441 caaaatcaaa caacgcctgc caaaaatctg cttttcccac agtcgctgcg ccatttgaaa
     7501 gagcaacaaa aaaaataatc aaaagaacaa aacagaccaa aagcaggagt ctatttttct
     7561 tcatagtcct cataattgat cctcctgctc ttttggcaac aaatcatagg ttagaaaaac
     7621 tggtttattt gttcttggaa gcgtgacaat cgttgcatca atctcaaata tttttttcat
     7681 acactctttt gtgatcactt cttcaggtgt accttctgtg atcaacttac cgtttttcat
     7741 tgctaataaa taatctgaaa aacgtgaagc atgattaatg tcatgaatcg acatcaagat
     7801 cgttgtttgt ttcatttgat tgatttcagt caataactgt aggatctcta attggtgtgc
     7861 cggatctaaa aatgttgtcg gttcatccaa gatcaagata tctgtttcct gagcaagcgc
     7921 catagcgatc cagacacgct gggtttgtcc accagacagc gttgcgatat cccgcatacg
     7981 aaatccagct agacctgtag catccaacgc ccactcgatt gcttcatgat cttgcgtgtc
     8041 catgccactg aacagttttt gataagggta tcgtccgtaa gatactactt cttctactgt
     8101 catacctgta gggtgttcac tagtttgtgc taataatgca attttttttg caagttcttt
     8161 tgaactgtat gtagagatat cagagttatt gacaatgatc tttcctgatt tcacaggtag
     8221 tatacgggta aaacttttca ataaagtaga ttttccactt ccatttggcc caatcaaact
     8281 agtgattttc ccttcaggaa tcgtaacaga catgttttca atgatttttt tgttttcgta
     8341 gccggctgca atgtcagctg ctcttaatct cgtcatttca cccatccttt cgctgaaaat
     8401 cattctcaat tatgacgctg tttcaacatt tcgtcaagcg ctactttcat atctatagcc
     8461 aaatgaagcg gttgacagcg ttcgaaatat caggaataat aaccaatgag aaagattctc
     8521 aattatgtta ggaggaaaga aatgaaatta aaaacaaaag tattcacatt atctagcata
     8581 ttgctgctgg ctggagtact gagcgcttgc tccccctctt cagataaaga aacagctaca
     8641 actgccaatt cgacgacttc atccaatagt actcatacag tcacagatac acttggacat
     8701 aaagtagaga ttccaaacgc gcccaaacgg atcatcggca gttacttaga agactatctg
     8761 attgcactag gggaaaaacc tatcgcccag tggactgttg gcagtggttc cattcaagat
     8821 tatctgcaag atgatttaaa ggatgtccca acgatttctt atgaccttcc ttatgagaaa
     8881 gtgttaagct ttgagcctga tttgctgttg atttcttctt ccgctactgt cgaaggcggg
     8941 aaatacgaac aatatagcaa aatcgcgcca acttatgttg taaaaaacgg aacagatgtc
     9001 acatgggaag aacaactaaa agatgtcggc aaagctttgg caaaagaaaa agaggcagaa
     9061 aagatcattt ccgattatca aaaatccatc aagaaaactc gagaagaact agcagataaa
     9121 atcgagaaca agacagctgc agtgttgtgg gtgaccaaca actcagcctt tatggtcagc
     9181 aaaaatcggt caagtggaag aatcgtatac gacgatctgg aatttggtgt tcctgatcta
     9241 gtggaagagg tctccaaaaa agccacttct gactggtctg ctgtttcctc tgaaaagcta
     9301 gctgaattag atgctgacta catcatcctt gtgaacagtg acaaagatgc tgccatgttc
     9361 aatgaaccaa gctggaagaa tcttaaagca gtgacagaca atcatgtact gcaattcgga
     9421 cctgaatcta gttggctata taacggacct attgccagtc aggaaatggt caatgatatc
     9481 aaagaaaaac taaattgatt ttaactaaaa aaagcatctt cacgattagt cgtgatatgc
     9541 tttttttctt attaacaaac tagacgcata gattttattc ttcttttgaa gcaaccctat
     9601 tttttactct ttcccatttt tgaaaacgat gggcatatag attttcttga tccgttatcc
     9661 cctcatactc ctctataagg gcgaagtctg aaaaatcaat attcccaata tacgtatcgc
     9721 cttcaaacgt gctatcgatc aatgttcgcc agatccgacc agtgtaagga agaagttctt
     9781 gaaaaatccg actaccaccc gaaacataga tatcttctga gatcgtttga ctatacgaca
     9841 aaagttcatc gatcgaatgc attacttccg cattttcttt cgtgcgaaaa tttggctgcg
     9901 ttgtcaacac gatgatatgg cgataaggaa gtgaaagatc ccccattcct tcataggtct
     9961 ttcttcccat cactaaaatt cgatccattg tatgttcgcg aaagaaacgc atatcatttg
    10021 gcaatcgcca tggtaacaaa ccatttttgc cgatcagacc atttttatct tgtgcccaca
    10081 tggaaataaa catattatcc gctccttatg attttagatg ttttgagata aaaaaagtct
    10141 caattctttg accagcaagg aattgagaac tttaaaaaca ttattttgtt tcacggtgca
    10201 aagtaacttt gcgttcgcgt gggcaatatt tttgtttttc caaacgatca ggattgttac
    10261 gtttgttttt gcttgttaga tagttacgtt ctttacaaga agtacactct aaagtgatgt
    10321 ttacgcgcat gtttttccct cccattttta caagaatcct ctaagatgac tctcagactt
    10381 gattatctta ccatgatttt ttctagattg ctagtgtttt tcacgatttt gttaagtaaa
    10441 actacttgac gataaataca acggttttac ttctcaaaca aaatcttttt ctttttgtat
    10501 aaataccctt acacttgttt atatagatta tgttttttaa taaaggggga tactaatatg
    10561 ccatggaaca tgaaggactt tcctgcatca atgaaaaact tagataaatt gacaagaaaa
    10621 aaagcaatcg acatcgcaaa tgcattatta gatgaagggt accctgataa ccgtgcgatc
    10681 ccaattgcca ttgatcaagc aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga
    10741 actttcgaaa aagaaaaaaa tccaagcaag acggatgaac acgatacgaa tcctcgtgct
    10801 ggaaaactgc ttgattcaga tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa
    10861 tctaaagggg caaaaaaggc aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa
    10921 ggaaagcaag tagcacaaaa caaagaatca actttgttca tttataaaaa agatgggaca
    10981 aaagaaaaag aaatcgatta ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac
    11041 aaccccctta tgttcgaaac aatggtgttc ctctgccaac tccgaaccat aagtcataat
    11101 atctataaaa cgggaagagc tgttttttga aattgtttct tatcactcag tgttaaacgg
    11161 taggagaaaa cctctttttt attgatattt gattttcatc aaacagtcac atagtcgatt
    11221 gcttctgctt gcggaatcgt ttcatctaca aagtagactt tataccatgt caagaaactg
    11281 aagattgtcc agatttttct ttgctcttct ttacgtcctt cgtgatggtc atctagcaac
    11341 ttcatgattt tttcttgatc aaaaaattct tttgcaaaat ctgcttcgaa caatgctttg
    11401 acttgttcgt agccgtgttc ttctttcaac caagctttga ttggcacagg gaatcctaat
    11461 ttcacacgat tcgaccattt ttccggaaga tgtttgttcg cagcttgtct gaaaatatct
    11521 tttgtgttgt tttgattcaa taaaaactta cttggtattt gttgagccaa tttcatgact
    11581 tcgatatcca ataaaggtac tcggacttcc aaagaacttg ccatagaaag tttatctgcc
    11641 tttaacaaga tatcttttgg catccattga tgaagatcga catattgcat cttattgact
    11701 tcatctttga ttccttctgt tttctcgtaa tgaacagtca tgatttcatc cacagatggt
    11761 gctgtttgat attttggtgt taacacttct gatgcttctg attcttcgaa cactcgagca
    11821 tgaccgataa aatattcttt tgcaggagca agtgattcat ataaatggat ccgtccatgg
    11881 aaatttttca tttttccgat tttacgtcct aaattatagc gtgttccttt tggtaatttt
    11941 ttcaatcctt gagcaatcac acggatgaat tttgaattcg tatggaatcc atacgtttgg
    12001 tatcccgcaa acagttcatc cgccccttct ccagattgga ctacccgcac actttgtgaa
    12061 gcaagatttg ctaaaaagta caaaggtacg caagaaggat ttgaatcagg ttcatctaag
    12121 tgatactgga tcaaagggaa tgctttgaac gacatgtctc cgtcgatcac tgctgctgta
    12181 ttatctaagt ccaatagttc ggttagtttt ctggcttcaa tcgcttcatt ataggtttta
    12241 tcatcaaacc cgattgagaa agaatgatct ggtttcaata cagaggttac ataactggaa
    12301 tcaaccccgc tggataagaa agaaccaact tctacatcac tgaccgtatg tgcttcaata
    12361 gaagcttgaa cggtttcgtc gattttttcg atccattctt gtcgggaatg cgtttctttt
    12421 gtttcaaaat ctgcatccca atattgctga atgtctattt ttccatcttt atacgtataa
    12481 taatggcctt caggcaaacg atagacatct ttgaagaatg tttcgccgtt taatggagaa
    12541 tattggaaag tcatataagg tttcaacgct tctttgttca attctttgtt aaaatcagga
    12601 tgaggcagaa aacttttgat ttctgaacca aacataaatg ttccgttcat ttctgcataa
    12661 taatatggtt tgatcccaaa gtggtctctt gcaccgaata actcattctt ttcattatcc
    12721 caaatagcaa aagcaaacat tccgcggatc ttttgcaata attctgttcc ccattcttcg
    12781 taaccatgca aaaggacttc tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc
    12841 aattcttctc tcaaaggctg gtaattatag atttcgccat taaaaataat gatctttgtc
    12901 cgatcttcat tgtaaatagg ctgagtaccg ccctctaagt caataatact taatcttctg
    12961 aatcctaaag caacatgttt atcgatatat tctcctgaac tattaggtcc acggtgaacg
    13021 attcgatcca tcattgtatt aataattgtt tttttgttgt ccttgtcgtt aacaaaacct
    13081 acaataccgc acatttaact ttcacatcct aatataaaat tactgtttat ttcattcata
    13141 tgcatcttat catagaataa atagcggacg aatattttct ctcttacaat ttcgtcacgt
    13201 gaaaacaaaa gatgaaagtt tctgttttta cagaaaataa agtcccaata tctaatttac
    13261 catgttttct tttttgaggc taccttattc tcttcgtgtt aaaaaaatat tagtggatgc
    13321 tatttccaaa aaaaccagca aaaaaagaca cgacctaaag atcgtgtcta attatgactg
    13381 tatcagaaaa tatgatacca aacggatcta ttcattaatt aattgacata gtttatctgt
    13441 tgctctttta tgactggata actgaccaat tcatgacgat ccaagtcttc tttattcata
    13501 tttacagcag taatttgact gtcttcatac gtacgataat aataaatccc tttatcaacg
    13561 ttacagcaag aagaataaat cgtatattcg tattttcttt ctccagtatc gcatactcct
    13621 ttttgttgct cgactgagcc tagtatatga aaaaattgac tgatactttc agattctgaa
    13681 tcacctgatg cagcattcat tttagtaaat gtggctttta caaatcgaga aacagaagag
    13741 agatctccag gtaagcccaa tcctcccatc ccacgactat aaacattcaa gctgatctgg
    13801 tttgaaaaat gattttctgg tgttccattt gatagcgagc gataattgtt cagattaaat
    13861 aattgataat caaatggcgg attatttgtc aacacaccga caggattatc atataaatga
    13921 agtccatctt tcatactttc aattactatc gatttttctt tatcagctaa taaccagtgt
    13981 aaaggtgaca agggtagttt ttcactaaaa tcgatattga ctagattgat agtagccaat
    14041 aattctttag cttcgtctat ggttgagcat tgccctagta tccatggaat aaactcaaat
    14101 ggtgcaacgt tatcttttcc ttcttgtaat tctttataat cagcattgcc aggaaaattc
    14161 agtcctgcca tactcaaccc tttttcattt gtagcctcat aatagagtgg atagttggat
    14221 accccagctg ctattcctat cattgcataa tgtttatcta aattttttac ctttctaaaa
    14281 tcgaaacgat aatttcttgg agtaatagtg accacttcat tataagacat ctcataatca
    14341 aaattccttc caaaataatg gtctttcgtt gcataagtga ttgcggtaca catcgtttcc
    14401 cctcctaaaa caaatcgttt ttacactttt atcttacatc tagtattttt tttatcaaaa
    14461 aaaggccaaa gaagattgat ttactagcat ttataaaaaa tagtactcag ggggaatcaa
    14521 aaaatattgt aggttaaaag tacaacaata aggggctgtg acaaaagtct tgtcacagcc
    14581 ccttattccg aacaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaac
    14641 gaaaaaatag gaaaagctat ttttgtttgg ccgttcttat tacttgatgc aggacagctt
    14701 cttcacaacc tctaatcttt gatataagga aggtacagga tttgaacctg cgcgccggta
    14761 ttagccggtt cgccggattt cgagtccggt gcattaccac tctgccaacc ttccgtaagt
    14821 actattttta aacttttttc acgaaatgtc aagcctattt ctaatagata aataacagaa
    14881 atcgcttttt ttagcaaaaa agtccattga cagtttgcag ccaacggact taccgatcaa
    14941 attcatactg atagaacact atctgattat ttcaggatta tctaatgcta aaatcgagtc
    15001 aattgtccgt ttgatcagtg caatgatttg ttcttctgtt ggatgcatga tgtttgatga
    15061 catcaatgac gctaagccag tcactgctac ccatgtacca gtatgcagag catcgacata
    15121 ttcatccgat aattgattgt atctttcatc ttctttaact atttcacgga aataattgta
    15181 tgaaaaatca tgcattttct ttccgtcccc atgttcatca atgtacaatg ccatgtaaag
    15241 actatgatgc ttttttgcaa agtagatata gttcaaaccg agatcaatga ttttatcacc
    15301 agtacgttca atagggaaaa catcttcttt tagttgttca aagatagaat ccagcaacgt
    15361 attttttaaa tcttccatgt ttttgaattc cagataaatc ggctgtgtcg aaatccccat
    15421 ctttttagca atatttctcg cagtaaatcc tttaaaccct tcctttgcaa caacatcata
    15481 agcagctttt agaatttgat ccttggtata gacttttctt ctcattaaaa ctcccccact
    15541 tctttactgc agtatatgtt gttttattgt accctatttt tagcattttt tgcaaacatt
    15601 ttactcaaaa cgttatattt taaatttttt ttcagtcttc tttaactttt ttttgcaaaa
    15661 ttaaaaaaat ttggttaaat tcaatgggtt cccttacatt tttagttttg acctaaatga
    15721 tagttgtcca aatctttcat ttcaagttat agttaaagaa aatgaaaaag aaggtgtgta
    15781 atggaaaacg aaactgacgt tatttatatt catcctcaaa aacgaatcgt ttcacagaaa
    15841 agaaagtatt tttatcttgg ttttacaggt gtttttttct ttttattggc ttgctttcta
    15901 atactcctac tgataattgg agtggattgc taactattct tacctcgcca agcaatcttt
    15961 tgacagatta ttttgcttta ggcggtttcg gaagcgcatt cataaatgta gggattttga
    16021 ccttactttc tgtgctttta gcttatcggc acaaggtgat tttaaacggt cccttatttg
    16081 cttcgattct aacagttacc ggtttctctt tttttggcaa aaacctttat aattctatct
    16141 caatcatttt aggtgtgtat ctatacgctg tatttgtcaa taaacctttt tctcagtaca
    16201 tcatgatcgg actgtttggt agcgcattat caccggtcgt tagttatatc acttttggta
    16261 tgcgttttcc tttactgata ggtattttat tggggaattt agctggcata gcaattggtc
    16321 tacttcttcc gcccttagct gctcaaactt tagttttcca tcgtggattc acactttata
    16381 atattggatt cacctctggg ttgattgcga tgacttttac tgctgttttg aggttattca
    16441 gctattcaat cgtcgaaaat acattggttt tcaatgaata tcactttcct ctcatttgga
    16501 ttatttttgg ctttttttcg ctgacagtcg gaattggttt ctattataat tcctttcggc
    16561 ttttggggat tcgagaaatt ttcgattcat ctggaaaatt gacaactgat ttcattgcta
    16621 attctggtat tggtgctact ttgatcaaca taggcttagt tggtctgatg ctgtcttctt
    16681 atgttttgct ggttggcggt cagttaaatg gacctgtcat cggtgctatt ttatctgcgg
    16741 tgggtttcag tgcttttggt tgtcatttaa aaaacagttt tccgatacta gtcggaatat
    16801 ttattgcttc tttgtttgga acttttcatg agatcaccag tactggcatg cttgttgcag
    16861 ctgtgtttgg gacaggtctc gcgcccataa gcggttttta cggcagtttt tacggtgtaa
    16921 tagctggtgt actacatatt gctcttgtcc ataacgtcag cactttgcac ggaggactaa
    16981 atctatataa cagtggtttt tcaactggtt tcgttgcagg tatccttgta ccgatactag
    17041 ataactttac cgctgtcaga aaggaaaaaa agacacttgg aaaaagaatt atcaaaaaga
    17101 atcatcgatg agcttcttac ttatttatat tcccatgaaa taactgaaat acgtattgga
    17161 gtagaatttg ctgaggaagg gcttttcata gagatacagg gtacggcaga aactcagcca
    17221 gaagatttag atgtacttgc tgatttgctg aacaatcctc gggaccctag tatggaaatc
    17281 tattatcaag aactattagg taatccccga catgatcggg aagattatca tctgttaggt
    17341 agtttgattg atgacgtgga aatcatgtat gcccagccga ttatcaatat taagatcttt
    17401 cgacgtgctt ctcattccta ttattaaccc tattttctat ttataaaaga ctgaatcaaa
    17461 aaagcagctc ctgaaagttc gattaaaatc tactttcagg agctgttcaa gcttgacatt
    17521 attgattttc caatccttca tacacttcat ctgttacagg ttctaaaaac tctgaattac
    17581 cagcagtgat cgccaaatgt tcaaaccaac tgtcttttgt cgcaccatgc caatgtttaa
    17641 tccctttgtg cgttacgacg acatctccag ccattaatcg tcttgctggc tttccttctt
    17701 cctgatacca gccttctcct ccggtcacaa gtaagatttg atagccgtct ttatggacat
    17761 gccagtgatt gcgacaacct ggttcaaaag ttacattacc tacaccaaca gaaacttcgg
    17821 gatcattcac taacatattc agatagcttt acccggtaaa tacttgagcg taagcttcgt
    17881 ttttttcacc tgctggaaaa atcacgccat tgtgtatttc ttcgtgtttt gtcgtcattt
    17941 acgcctcttt cttatcttcg ctattttctt ctaaatagat ttcttttgct aaactaaaag
    18001 ctgaccaagc ttttggccat cctacataaa aagctaaatg ggtaatgatt tctgctattt
    18061 cttcactagt aatgccattt tgcttgccaa tcttcagatg agctggcaat tgttcaaaat
    18121 ttcctcctga aattaatgca gatatggtga tgatactacg ctgatgagca gataattctt
    18181 ctctggacca tacttgtcca aataaaacat catcattcaa ttctgcaaat tttggtgcaa
    18241 aatctcctaa ttgatctcgt cctgcggttt gtttttctgc catttctctt attctccctt
    18301 cacgactgtt aggtgattca actatggttc taaggcaact ttattttcta ttgtttcttc
    18361 gcatttcatt ttaaatgtag tctttcagcc tgtccaatgc ttagtttatt cagtcgatat
    18421 gctttttaag catagttgct aacgcttttt tta
//
LOCUS       DAZKOK010000055        18235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000055 DAZKOK010000000
VERSION     DAZKOK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.055"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1690
                     /locus_tag="ACG90W_001604"
     CDS             29..1690
                     /locus_tag="ACG90W_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0477964.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1760..2107)
                     /locus_tag="ACG90W_001605"
     CDS             complement(1760..2107)
                     /locus_tag="ACG90W_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0477965.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2191..3132
                     /gene="opp3b"
                     /locus_tag="ACG90W_001606"
     CDS             2191..3132
                     /gene="opp3b"
                     /locus_tag="ACG90W_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0477966.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3133..4176
                     /locus_tag="ACG90W_001607"
     CDS             3133..4176
                     /locus_tag="ACG90W_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477967.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4182..5252
                     /locus_tag="ACG90W_001608"
     CDS             4182..5252
                     /locus_tag="ACG90W_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477968.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5242..6195
                     /locus_tag="ACG90W_001609"
     CDS             5242..6195
                     /locus_tag="ACG90W_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477969.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6207..7217
                     /locus_tag="ACG90W_001610"
     CDS             6207..7217
                     /locus_tag="ACG90W_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0477970.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7502..8323)
                     /locus_tag="ACG90W_001611"
     CDS             complement(7502..8323)
                     /locus_tag="ACG90W_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0477971.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8420..8698)
                     /locus_tag="ACG90W_001612"
     CDS             complement(8420..8698)
                     /locus_tag="ACG90W_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477972.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8966..10975
                     /gene="metG"
                     /locus_tag="ACG90W_001613"
     CDS             8966..10975
                     /gene="metG"
                     /locus_tag="ACG90W_001613"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0477973.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11454..12041)
                     /locus_tag="ACG90W_001614"
     CDS             complement(11454..12041)
                     /locus_tag="ACG90W_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477974.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12216..13538
                     /locus_tag="ACG90W_001615"
     CDS             12216..13538
                     /locus_tag="ACG90W_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0477975.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13693..14469
                     /locus_tag="ACG90W_001616"
     CDS             13693..14469
                     /locus_tag="ACG90W_001616"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0477976.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14462..15031
                     /gene="rnmV"
                     /locus_tag="ACG90W_001617"
     CDS             14462..15031
                     /gene="rnmV"
                     /locus_tag="ACG90W_001617"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0477977.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15024..15908
                     /gene="rsmA"
                     /locus_tag="ACG90W_001618"
     CDS             15024..15908
                     /gene="rsmA"
                     /locus_tag="ACG90W_001618"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0477978.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16359..16988
                     /locus_tag="ACG90W_001619"
     CDS             16359..16988
                     /locus_tag="ACG90W_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0477979.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17112..18221)
                     /locus_tag="ACG90W_001620"
     CDS             complement(17112..18221)
                     /locus_tag="ACG90W_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477980.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5949 a         3299 c         3739 g         5248 t
ORIGIN      
        1 tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc
       61 ggtatgcggc cttgtacttg ctggatgtta tggcggcagt tcagccacaa gcagtagttc
      121 agcaaaaagc ggttcagcag atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat
      181 gcctacagct gacttatcag ttgcaacaga tacaatcagt tttacagcat tgaataacgt
      241 ttatgaagga atttaccgtc ttgatgaaga cagcaaacca cagccggcag gagctagcga
      301 gttggcagaa gtcagtgaag acggattaac ttacaaaatc aaattacgag aagacgcaaa
      361 atggtcaaat ggtgatcctg ttacagcagc ggattacgtt tatggctggc aacgaacagt
      421 agacccagct acagcttctg aatatgctta cctgtatgcg ccagttgaaa atgcagaaga
      481 cattacagct ggaaagaaag ataaatcaga actagggatc aaagcagttg gtgactatga
      541 attagaaatc aaattgacga aacaaacacc atatttccaa tatctgcttg ctttcccatc
      601 atttttccca caaaaacaat ccgtagtgga agaaaatggg gatgcctatg catctgcaag
      661 tgataaagct gtttataatg gaccattcac attagaagat tttgatggac caggaacaga
      721 tacagaatgg acttataaga aaaatgatca ttactgggac aaagatacag taaaactttc
      781 tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca ttgaacttat tcaaagatgg
      841 ccaagcagat gacgtgatct tgtctggtga gttagcacag caaaacgcaa acgatccagc
      901 ttatacttct gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa
      961 ttcaccattc aacaatgtga acctaagaaa agctatttct tattctatcg atcgtgaagc
     1021 tttagtaaaa caagtactag gtgacggatc agttgtctct acaggattga tcccatcaga
     1081 tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc
     1141 ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga
     1201 ttctttagaa tttgatttga tggcttctga tgatgattca tcgaaaaaag taatcgaata
     1261 tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc
     1321 attctctgta cgtcttgacc gctcaacttc gggtgatttt gacgcagtac taggcggatg
     1381 ggctgctgac tatgcagacc caagcagctt tactgatttg tttgtcacag gtaattcata
     1441 caaccgtggt cgctggagca atgcagaata tgacgaagca gttaaagcag cttcatcaac
     1501 agacgctggc gatgaacaag cccgttggga tgatttccaa aaagcaatca agatcatctc
     1561 tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa
     1621 aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg gattacaaat ggacatacgt
     1681 tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca
     1741 acctcgttct catattcact tatcctaaca aagaaaaaag catgaaaata gcagaaagag
     1801 caattagtat tccggcaatg atcagccaaa agctgtacat accgtagctg gcagacgaga
     1861 gcgaggaaaa ttctggtttt ttctttcggt tccttgttcg tgcaagatgg accgatcgct
     1921 gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa accgccgata atcagaaacg
     1981 gcaatgacca taagaataga tcattagata attgaatgag tgtaaaggaa tggttgatga
     2041 gattttttat taaaataacc agaatcagta gaaaagctac tgcgtagggg atagattttt
     2101 ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc gaaacatctg
     2161 tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata tgtattaaaa
     2221 cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt tttcttgatg
     2281 caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga aacaatcgag
     2341 atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg gatttatctt
     2401 tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca gccagtcgca
     2461 catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc aattatcttt
     2521 ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa tacatgggca
     2581 gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt tgtatttgct
     2641 gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat
     2701 ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct tgctgactct
     2761 gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt ggaattagca
     2821 aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg aaatagtttg
     2881 attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg atcactggtg
     2941 gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc aatcacaaca
     3001 aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct gatttttgtt
     3061 attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg
     3121 agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga tatccctgcg
     3181 gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat tactgcacct
     3241 tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt
     3301 tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt tgtttctcct
     3361 catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat tccgggaatc
     3421 aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga taaatatgct
     3481 caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt gggacgtgat
     3541 gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt tattgctgca
     3601 ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt aggcggacgt
     3661 gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa cctcgttgtc
     3721 atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc tgctatggcc
     3781 atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat
     3841 caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa
     3901 catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag tattccatct
     3961 gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc tccgtcagct
     4021 tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc ttatctatta
     4081 tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact tgctgatgga
     4141 ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa attttagaag
     4201 tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg attcgcggtg
     4261 tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct ggaagtggaa
     4321 aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat atagatgctg
     4381 gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa
     4441 tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta gatccgacga
     4501 tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt tcaaaaaaag
     4561 agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa gctgagaaac
     4621 gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc gtgatcgcga
     4681 ttgcactggt atgttatccg gaaatactga ttgctgatga acctacaaca gcgcttgatg
     4741 tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa atcgatacat
     4801 caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg
     4861 tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc tacaacccgc
     4921 aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt gcgaacgatc
     4981 gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa ggagacgcat
     5041 tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca ccattttttg
     5101 aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca cccaaagtga
     5161 caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa aaacatactt
     5221 acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg attgaaacag
     5281 tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag ctttcacatt
     5341 tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc cactactggc
     5401 cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga cggtcaagat
     5461 ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc
     5521 tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat cattgccgaa
     5581 ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat
     5641 ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca tgaattttca
     5701 ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc gcgttttatc
     5761 atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg
     5821 ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca tgatttgtcg
     5881 atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt attagagatg
     5941 ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag tttgttatct
     6001 gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa atatcaacct
     6061 gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca ttttgtctat
     6121 gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa ggaatctctt
     6181 ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt atgtatccga
     6241 cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt acctataacc
     6301 atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat ttttcgctca
     6361 cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt cttgctttag
     6421 caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga cgtgccagct
     6481 tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc
     6541 gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga
     6601 taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct gctcgatata
     6661 atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag
     6721 ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct cgacctctag
     6781 aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca cagcggaaat
     6841 tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag gcaagcaatt
     6901 atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac cagcgagaag
     6961 attatcttga acaattatct gggaaattgg tcaaacaata tgactactta tttgtagaat
     7021 cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat aaattaataa
     7081 caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc aataccgacg
     7141 gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt
     7201 cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt agccttgtta
     7261 ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa agctcactaa
     7321 ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt ctgctattgt
     7381 taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc
     7441 acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt tgctcacaac
     7501 cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg attcctacag
     7561 gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg gaatgatcga
     7621 tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc gtaatcgtga
     7681 aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg actacacggt
     7741 tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg ccagaagact
     7801 ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg atcttgttac
     7861 tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg attagcggtt
     7921 cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga tctcgattag
     7981 gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca ccagtttctt
     8041 cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca aagccaccag
     8101 gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa actttcagct
     8161 gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag ggcttgtcat
     8221 attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt gctttatgtt
     8281 cataatataa tttttcttct tctttagaag caaaacggtt catctgacca cctccattga
     8341 cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa ggatttactt
     8401 gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga ttgaataaaa
     8461 agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca aagaagccac
     8521 aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc ataaaactgt
     8581 tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag ctgaatatta
     8641 aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga
     8701 ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga ggggagaaat
     8761 aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat tcaacctatt
     8821 catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc tttttcattt
     8881 tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac gaattcgcat
     8941 catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata tcactacgcc
     9001 aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga ttgcatgtga
     9061 cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca ctggagtgga
     9121 tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata
     9181 tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata tcagttatga
     9241 taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa tatttgatcg
     9301 tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact ctgtttctga
     9361 cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg aaggtaacgt
     9421 gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag aatcatactt
     9481 cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac atcccgaatt
     9541 catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac caggattaga
     9601 ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga gtgatccgaa
     9661 acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac taggatatgg
     9721 ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa
     9781 agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc ttgatttgcc
     9841 tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa aaatgtctaa
     9901 atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt tagatgcact
     9961 ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga
    10021 ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat tgaatcgaac
    10081 gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct cacttgtcac
    10141 tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt accatgatgc
    10201 aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga tttctcgtgc
    10261 aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag aaaagaaagc
    10321 tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag cgatattact
    10381 tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat tggactctga
    10441 aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa aagtagttgc
    10501 aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt ttattcaaga
    10561 aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc cagaagaaac
    10621 agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa aagtagaact
    10681 aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt tgctgcaatt
    10741 ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta
    10801 tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg
    10861 aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac cagatggctc
    10921 tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg catagaaatt
    10981 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
    11041 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
    11101 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
    11161 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
    11221 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
    11281 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
    11341 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
    11401 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
    11461 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
    11521 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
    11581 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
    11641 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
    11701 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
    11761 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
    11821 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
    11881 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
    11941 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
    12001 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
    12061 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
    12121 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
    12181 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
    12241 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
    12301 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
    12361 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
    12421 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
    12481 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
    12541 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
    12601 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
    12661 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
    12721 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
    12781 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
    12841 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
    12901 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
    12961 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
    13021 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
    13081 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
    13141 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
    13201 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
    13261 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
    13321 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
    13381 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
    13441 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
    13501 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
    13561 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
    13621 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
    13681 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
    13741 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
    13801 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
    13861 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
    13921 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
    13981 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
    14041 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
    14101 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
    14161 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
    14221 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
    14281 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
    14341 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
    14401 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
    14461 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
    14521 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
    14581 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
    14641 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
    14701 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
    14761 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
    14821 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
    14881 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
    14941 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
    15001 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
    15061 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
    15121 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
    15181 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
    15241 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
    15301 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
    15361 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
    15421 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
    15481 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
    15541 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
    15601 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
    15661 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
    15721 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
    15781 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
    15841 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
    15901 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
    15961 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
    16021 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
    16081 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
    16141 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
    16201 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
    16261 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
    16321 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
    16381 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
    16441 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
    16501 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
    16561 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
    16621 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
    16681 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
    16741 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
    16801 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
    16861 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
    16921 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
    16981 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
    17041 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
    17101 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
    17161 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
    17221 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
    17281 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
    17341 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
    17401 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
    17461 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
    17521 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
    17581 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
    17641 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
    17701 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
    17761 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
    17821 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
    17881 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
    17941 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
    18001 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
    18061 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
    18121 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
    18181 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacac
//
LOCUS       DAZKOK010000056        17543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000056 DAZKOK010000000
VERSION     DAZKOK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.056"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1341)
                     /locus_tag="ACG90W_001621"
     CDS             complement(67..1341)
                     /locus_tag="ACG90W_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0477981.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1520..2167)
                     /locus_tag="ACG90W_001622"
     CDS             complement(1520..2167)
                     /locus_tag="ACG90W_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0477982.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2175..2861)
                     /locus_tag="ACG90W_001623"
     CDS             complement(2175..2861)
                     /locus_tag="ACG90W_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0477983.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2867..3940)
                     /locus_tag="ACG90W_001624"
     CDS             complement(2867..3940)
                     /locus_tag="ACG90W_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0477984.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4162..4836
                     /locus_tag="ACG90W_001625"
     CDS             4162..4836
                     /locus_tag="ACG90W_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0477985.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4841..6181
                     /locus_tag="ACG90W_001626"
     CDS             4841..6181
                     /locus_tag="ACG90W_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0477986.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6330..7316)
                     /locus_tag="ACG90W_001627"
     CDS             complement(6330..7316)
                     /locus_tag="ACG90W_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0477987.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            8040..9071
                     /locus_tag="ACG90W_001628"
     CDS             8040..9071
                     /locus_tag="ACG90W_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0477988.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            9180..10712
                     /locus_tag="ACG90W_001629"
     CDS             9180..10712
                     /locus_tag="ACG90W_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0477989.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            10851..11663
                     /locus_tag="ACG90W_001630"
     CDS             10851..11663
                     /locus_tag="ACG90W_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0477990.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            11653..12138
                     /locus_tag="ACG90W_001631"
     CDS             11653..12138
                     /locus_tag="ACG90W_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0477991.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            12161..12706
                     /locus_tag="ACG90W_001632"
     CDS             12161..12706
                     /locus_tag="ACG90W_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0477992.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(12779..14422)
                     /locus_tag="ACG90W_001633"
     CDS             complement(12779..14422)
                     /locus_tag="ACG90W_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0477993.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(14551..16779)
                     /locus_tag="ACG90W_001634"
     CDS             complement(14551..16779)
                     /locus_tag="ACG90W_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0477994.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(16812..17291)
                     /locus_tag="ACG90W_001635"
     CDS             complement(16812..17291)
                     /locus_tag="ACG90W_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0477995.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         5166 a         3345 c         3215 g         5817 t
ORIGIN      
        1 tggcgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg tgcgccgcat
       61 atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc gaaagttata
      121 aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat tcagcccctc
      181 agtgacgccg tttgagtggg gcaccttaaa ggcatttgaa atacccgatt ggtagcgatg
      241 gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg ttttaagggt
      301 ttcttgaaag ccttccgtat ctcgtttaac gaaataatag cgtaataatt gacaaaaatg
      361 atacgcagtt cgcaattctt cgttgtagct taacagctca tcaacgattt ctgtttgact
      421 aattggtcgt ttaaacagtc gatgataatg ccgtttgagt ggctccaatg tcgtggaatc
      481 tttgagtaag agtttccaat atcgcttgag ccgacggtac ttcttctgat tttcagagtg
      541 tgaggttcga aaacgattca tgatctggac acgtaattga ttgaagctgc gattgatgtg
      601 ttggacaata tgaaagcggt cagttacgat ctccgcattt ggaaagactg ttttaagcaa
      661 ctgacaatag ctggcattca tgtccatcac taggtacttg acctttaaac gggcctttct
      721 aggaaagcgc aggaagtatt gagttagttt gtaaagtctg cgatcttcaa ggatcgtaaa
      781 tagctgattg gttgtcccat caacacaaat aaaactcatg gaaccagaaa cagacttcat
      841 tgatttgaac tcatccatac acaacaccgt cggaaggaag ttcatacgtg gctggtaatg
      901 cttcgttgct tcacgcaaga tccgtgtcac cgtcccatcc gagacgaagt acttcttagc
      961 gattagttta cgtgattgat tttcgtacag ttccatgaga atcgcctgct tcagttcctt
     1021 tgttagttga tgatgttcat caactaagtc cgttttcgca gaaaaggtcc gtccacaatc
     1081 atggcagaga aaacgggtac gttttaattc taaaatagtc aacttttctt tgactttcaa
     1141 catctgagtg cgtgtcgtat aggaaccgtt gcggataata cgaatcgatc cacagtgttc
     1201 acaatgggaa ggtgagtcat ccaatctccc tttaataaac tgcgcttgaa cgcctttata
     1261 ttctttttca atcagccatt cttcatcaaa aaataaatgt ttatctgtta atccgagtaa
     1321 ttttctagta tgattatcca ttgagagact cctttggtag ggtgacgttt tggtcgacta
     1381 aattttacca aaagtgagtc tctttttgag ttgaaacgaa gaaaaaagtg ttagtagaaa
     1441 atcgctttcg attttccacc aacacaaaat attatagaac cataaaaatt tcaggacaaa
     1501 tacacatagc tgtttctttt tattgtattc cttcagcaat ccaacgagat ttatagcgaa
     1561 agtcttttaa caactcttct tttgtccaat tatttgcaat gagatccgtc aaagtgttca
     1621 ttacgagggt catggaaaag tataggaaac taactgcttc ttccgacgat tgaatggaga
     1681 aatgattgat tcttaataat tccagccatt tttcgttcat tggagaatca gccgatagtt
     1741 ctctacgttt ggttgtctgc tgatcatttc gctgggttaa caagtataac ctttgccaat
     1801 agatactttc gtgatctaag tcgcagcacc actctaaata tttttcgatc cccaaaaaga
     1861 aatgttgttt gttttcgcta atgaatttta agatatcttg atggactatg tgagaactgc
     1921 ttttgatcgt atacaaataa gcatcttcca aatcttgaaa atatttataa aaagcaccgc
     1981 gcgacatccc catcttgtca acgatttctg agactttgac ttggctgatg tgctgtgagc
     2041 aaaatatctc cagtaatagg tcatcgattc tctgtttctt tgcgttcgat aaattgaaaa
     2101 aagtttcttt tggcatccac atctctcctt tatctctgat tatacgtgga tagcccttca
     2161 ttgtcatgaa ggattcattc cttctttttc tcttcttctg tcaagtaccc atcttccatt
     2221 cgataaacgc gatcactcca ttgaatcata cggacatcat gagtaaccat aatagtcgct
     2281 ttttgtttct catgagcttc tttaactagt aatttgacga cttcatatgc atgatctgta
     2341 tccaaactag ctgttggttc gtctgctaag atcaagcttg gctcattata taaagcgcga
     2401 gcaattgcca ctctctgtct ttctccacca gaaaggtctt taggataact atttttaagt
     2461 gactggatat ctaaagattg cagcaaggaa tcgatttttg ttgaatcttc tttttttgaa
     2521 gtaacttggt caactagttc aaattgttcc tttaccgtta aaaaaggaat caaattagag
     2581 ctttggagaa taaatcctat ttctttaaag cgtaaatctg ctcgtttttt ttctggaagc
     2641 tcagttagtg agtgaccgtt gatcactatt tctccggaag tcggtgtttg caatcctcct
     2701 gaaatggtaa ggaaagtact tttacctgaa ccagaaggac caatgatgct gacgaattct
     2761 ccttccttta cataaaaatc aattgctttc aaagctgaga cctttgtatg accagaacca
     2821 aatgttttgg taactttttt catcgttaat atttttttca tttttattaa cctcctattg
     2881 ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa caatccgcct agcattgcga
     2941 caatgatcaa aatcccacta tatagcaacc attgtgacca aaaaacagca aaaggcatcg
     3001 cgcttggtaa aatcagacta gtcaaataag caaacaaaag agcaagcaga acgcctacca
     3061 ccccaacaat aaaggactgt gctactaaag atttagcgat gaagaaattt ttgatccctt
     3121 gtgctttcat tactccaaaa atagcagtct tctgaagtgt catcacgtac atgaaaatac
     3181 cgaccacggc agcaaccact aagaacaaaa agtatatcat cgcatttaag gtcatatttt
     3241 gggcagaata gccaggaata ctttcaatga atgtcccaat agttaataac tgtagaccct
     3301 tcgatgcttt ttctttcgaa attttagctg aatcctttat tacgatccca ttgatcggct
     3361 gttcttcttc agaagcaaac ggctgttgtc catacttcaa agcagtccac gtatctaggg
     3421 acgcatagat gacgggagaa actgtgtaat aagtttcagg aaagataccg acaatcgtca
     3481 aaggatcgtc gtaactgcca attttgatcg tatctccaat tttatagcct gaatcagcca
     3541 gattttgaga gatgatgatt tcattctttg ttttaaattc atgccctttc gtgagttttg
     3601 gtaataaaaa tgcttctttc gttgtgccga aaactgtaac attttctttg ttttttcctt
     3661 tgattgctcc gctgtacaat ccgattggtg ctttctctcc tccttcaatg tatttcagat
     3721 ctcctcttgt taattgtgaa gctgcaaata cttgatttgc atcctccgac aaaacaatct
     3781 cttgtgcgtc ccaatcatcg attgcttttt taaactcttc tgaaagtcca ttcgctagtc
     3841 ctgacaacat gaatacgaca tacgcaatca agaacataat accgacgatc aagccatatc
     3901 gtaatttaga atagcgaatt tctttgatag ctagaaacat ttgtttttcc tccctatgat
     3961 agattgatag tgacaaaatg tcacttgttg aatagtgtaa ctctaaagtg acaatttgtc
     4021 acttgtgttg tttttttctt atcaataaat tgtctaagac atctttcgat aaaaccttaa
     4081 catgagtcat aaaaaattta tttaagcctt atttatgctt tccatgataa actaaagtga
     4141 ccaaatcaat gaggtgggaa aatgattaaa ctattagtag tagaagacga taaagtatta
     4201 tccaataata taaaagaaat actcgctgca atcggcgaga taacacaagt ttatgatggt
     4261 agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc ttgcaatctt ggatttgatg
     4321 atgccggaaa tgaatggata tgaagtgtta gcaaaaatac ggaaagaaaa aatacagact
     4381 ccagtattga ttttgactgc aaaagatagt ttggaagata agattgaagg atttgaacga
     4441 ggtgcagatg attatctaac aaaacctttc tatcgagaag aactgatcat gcgcgtgaaa
     4501 gcattattga aacgctcgct aggtctcttt gatgaaaatg ttctgaagta taaggatatc
     4561 agttgtgatt taaaaagcaa tgaagtcttg tataaagaac aagttttgcc catacaagga
     4621 aaagagtttg aattgctcgt ttatttttta caaaataagg gaatcatttt gacaaaagag
     4681 cagatatttg accggatctg gggatttgat tctgatacga cgattacagt tattgaagtt
     4741 tatatgagca acttacgaaa acatctgcga agtactggat tggataaaga aatcaaaacc
     4801 ttacgtaatg tcggttatat actgcaaagg gtgtgatgca atgaatggag atattacacg
     4861 aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca tttgctttta tcttttttct
     4921 tttgggattc attactttac agatattaaa ccaatctgca tacagagaaa cagatctgtc
     4981 attagagaaa ataaattttg attctcgaac agtacaatta gagatacaaa gatatcagca
     5041 aggcgatccc tttttaaacc cgactcaaac ggaacaaaac agaacaaaca tggaacagcc
     5101 attaatggaa ccatcaaatg accgttttaa cactcaagtg atcttgtggt ctaaaaatgg
     5161 tgagatattg aataaagcag ctattggtgg gcgtttcaac caaatcaaag aattaaaaat
     5221 ggatacgaaa aatctgaata ccatccaaaa tattgaatta aatgaagaca gtggctcatc
     5281 tgccctctct ttccgttcga ttacgaaaaa agcggagacg aatcaagatg gcgttgccta
     5341 catccaaatt ttagaaaata caaatcaaat caagaattca ttagcgactt ttcgcacgat
     5401 tatgattttg tgtatgattg ttttttggat tctctctatt ggtatcagtt attacttgtc
     5461 tagtctaagc atgcgcccta ttattctttc ttggagaaga caaaaagaat ttgttgagaa
     5521 tgcttctcat gaattgcgca caccgttaac gattattcaa aatagtttgg aacatctttt
     5581 taccaagcct aatcatacta ttattgaaga atccgaaagc atcgctcaag cattgtcaga
     5641 gacgaggcgc ctcactggat tgacttcgga tttgctgaca atcgctcgga atgattctga
     5701 tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa tatatcgaac aacttgttaa
     5761 accatttcaa gagatggcca tcatagatgg aaaagagttt attctccata acgatgcaac
     5821 attagaagta ccaattgatg aaaaaaaaat ccatcaagtt ttggttattt tgctggataa
     5881 tgcattaaaa tatacgaatc cagaagataa gatcattttg gattcatctg ttaaccataa
     5941 agaatggctg attcacgtaa agaatactgg cccttctatc agtgaagaag atcaagcaag
     6001 gatttttgat cgattttatc gggaagatca atctcgggcg aaagagactg gcggatacgg
     6061 gcttggcttg gcaattgcaa aacaggtaat cgaacagcat catggacaga tttctgtatc
     6121 caattatctt ccacaaggag tcgattttaa agtccaattg ccgttgaagc ctaaaaaata
     6181 atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc acagcccttt tttgttgcga
     6241 ataaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttctt taagctgacc
     6301 acttctgtca caacctcttt agcttacgct tacgattttt tattgcggtc ttcaatgtgt
     6361 acatcaccgc ttgtgtcaat atccaattct tctcggctaa catcttctgt cactttttca
     6421 acatcttcat gttcttcttt atgaatccca acttcttcac gaataactgt atgtttgctt
     6481 acatctactt gctcttcttt gattgggata gtaatcgtgt catcttccaa tttttcgtct
     6541 gttccctgtg aagattgatc tgttactggt ttgcgttcga tgacgatttc ttcacgtttg
     6601 accggtactt ctaccgtttc agtatcgttg accacatgtt tgtggatgtc gacttctcca
     6661 gttgtcacat catgtgtatt tacatctaat ttttcttctt ttagttgaat tgtatcatca
     6721 tctagatttg gtttgcgatc gatgtcttca gtcttgcttg tacccattgt atcagtctcg
     6781 ccaggtacgt ccatcggatc agttcctggt gtcatattca atggatccgt tgtattttga
     6841 aagcctgtcg tgccacctac gtccattgga tctgttccag tgccagtttc taatccagtc
     6901 cccattgtgc ttgctgggct aacagtatct tgtgtatcca ttgtatcttc tgttgtttct
     6961 ggctgacgat agccttttac tgtgatgaca aattttccat cagaaatatc tcctctatat
     7021 tgatataaag gatcattttc tggtgtagcc gtctcagaat cataggtatc aaaagaaaaa
     7081 gtatctttta ttttttccca taaagaacgg tcttcttcac catccatccg attttcatct
     7141 gtttcaacat ctataccaga caatccgtca aatcctagtc tagatgctgc ttcatcagta
     7201 gtataaataa cgatatcatc tcgttcatac ccttcgttac gcaaccgttc aattactgat
     7261 aatgcttctt ccctcgtact atagcttccg atgaccgtta aatttttctc ttccatgtaa
     7321 attcctccta gcaaatagat ttatgattgg tatattgtta cttataaatt tattctatag
     7381 gagacgattg ttttctacaa atatttagat tggaatttga ttaaaacaat ttaattgaaa
     7441 tttgaaaaca tttatgttaa tgaaagtcat attctaataa aatgtcattt gtttctatat
     7501 gaatttttat gaaaaatttc atataatttc cactcgttgt gatggtttaa tttatccaat
     7561 ccatcaataa gcaaacaact tatataaaaa aggattaatt ctcaatataa ttgctcctac
     7621 catagaataa atcttcatgt aaagtaatga agtaccaact atttctaata cttctttaag
     7681 aattatccca acaatatcgt tagacaaacc attattgaat aaatggagag ccaatcgatt
     7741 ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag agcctcctgc cattagaacg
     7801 aaccaaatcc atttcattgc tcatgaagta accacatagc taaaaagagg ataaatcaat
     7861 taaaagatca aaagaatgac tgcactaata aaataactaa aaatgtatct tcttacttca
     7921 tatcttttag ctagtactta gttaaaaatt tgcttcctaa gatcaagaca aactgtctat
     7981 cttcatttga tgaaatgcgg taaaatatag aaaatgaaca ctattggagg gaatttgtaa
     8041 tggacagcaa aactagaaaa caactgcaag aaaagttaaa gcaacgtaca cacttgctcc
     8101 cttccgtctt tcaacagttt ttagaagaaa atcgttcttc cctttcatta ggcagtcgct
     8161 atgaatacgc aaaagatttt catctgttta ctgattattt gagaaaagta ttttctgaag
     8221 aagtggatga tcagctgatt atccatttag aaaaacaaga ttatcagaat ttcttagcta
     8281 aaatctatcg acatactcgt tcgttccaaa ctacagcgaa ccaagaaacg gagcaattgt
     8341 ttgaaaatag ctattacggg atttcaagga agcaatattc attaaatcat tttttacgat
     8401 ttttgtatca aaaaggactg attgaagaag agatttctct tttgggtaca agtttaccgg
     8461 aaacaaccaa agcagctccg cgatatatcg atgcagaact tttcaaagat tttgagcatt
     8521 tgtttatccc gattgaaggc tttcaaacaa gcagagaagc tagctttgaa gaaaagaata
     8581 gaccgaggaa ccttgcaatt actgccattc ttctgtattg cggattaaag atccatgaag
     8641 tagtagaact taaacgtaaa gatgtatctt tgaccaagca gattcttaat ctgaatagaa
     8701 aagaaaacag actgaataaa gtaccaatac caaaagaagc gatgccattt ctggagaact
     8761 attttaagtt aacagcacaa aatacggacg aaaattcttt tgtttttcgt tcctcacatg
     8821 atcagcaaat ttctcctcgg actattcgcc aaattttgag caaaattaca gtatataaaa
     8881 acgtatcgcc agaactatgc agaaaaacat atcgttatta tgcagaacta tatttagatg
     8941 atgctgacgc ggtaaattat ttatgtggta atcgatacct ctcatcccat tcttttcaag
     9001 atatctggga aaagatggtt gatttttctt atcacgaatt agcggcattc gttcgggttt
     9061 cttccatata aaacaaatgt atgatctttt tttcacaata aactatgtta ttatataaag
     9121 gattatgtta cgattatctt gaatttgtat attacttaac aaaagataat ggagtgtgaa
     9181 tgatggagac cataacgtat tcccctttga acttatttac gaattttcaa gaagctgcag
     9241 ctaacttttc cgagaccccg attatctttg atgagccact ttcagctttc ccagaattag
     9301 gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt gaaacgggct tatcaattgg
     9361 ctcatcttgg agtgaaatcc ggagacaaag ttattattta taagagttcc gcttttgaca
     9421 cataccttct agctgtttct gtgtcttacc ttggtgcagt gcctgtcatg acttcttatc
     9481 atctaccaac aacaacaatg gaagtattta tcgatcgttt agaagaccct ttcattttat
     9541 ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa tggaacaaaa tcaaaacaga
     9601 ttcctattct ttatttaatg gaacaacctg ctttgtctgt ttctcaatca tttttagaca
     9661 aaaatgaaat ttcttatatg acccatactt ctggtacaac tggtatccct aaacttattt
     9721 gtcattctgc tcattctatg ggatggcgga cgaaatggca aaaaacagtt ttcacaaaaa
     9781 tttcggaaaa gaaattaatc gcttttcaca tctctccagt gcattccaga ttcaatattg
     9841 gtatctcttc tttgatgtcc atgggatttc ctatgatgcc tttagcaaac gcacaaagtt
     9901 caaaagttgt tcatatgttg gaagctcacc ggccaattgc actagagacc catccgaata
     9961 actttgttca gtggcgtttt actgctaagg agcatccaga agctttttcg ggtatccgat
    10021 actatcactc cacttttgat gctataaaca accagacaat gaagacattt ttgcgaacgt
    10081 cgatggcaca ggatgcggta tttcttcaag tatatggcca aagtgaatgc ggaccaatga
    10141 ttcttaaggc tcatacacta gagagtttga aaacaagtga tgcaagagat atgggcgttg
    10201 gactggaaga catgacaagc gctcggatta cagattcagt tgggaatgtc ctacctgaaa
    10261 acacagatgg tcatatacaa ttactgtcta aaggacgggc tttgacttat tacaaggaag
    10321 atgcacggtt ccaggaaaat gtttatggcg attggtggga tagcggagat tacggtttta
    10381 tggataagca tggccaccta tttttgaaag atcggcaagt ggatttgatc gaaacgatca
    10441 atagtacatt agctattgaa gactttttat tagattcgtt agattttttg gcagaagtag
    10501 tgattgtaag ggataaaaat cattctccac aaccgattat tgctttagct cctgataaag
    10561 aaatggattg gaatcgatgg tgggagcaag tccatgaact tcctcgcttg aacgtcccta
    10621 tcatccgtga ttttgatgca atcccgcgaa cagctacaat gaaagtgcaa cggcttcaaa
    10681 tcgagaaaga attaaaatca caaaagtcct aactatttaa ggaaagaaag atccattttg
    10741 gtctttcttt tctttttgtt tttttaaatt ggatgagata aaaatatgcc aattggctgt
    10801 ttttcttttt tgaaaatttc gttatactct tattcatgga ggaaaatgtt atgaaaagaa
    10861 tcttgacaat tggcggttct gatccatttg ctggtggagg aatccaaaca gatttaaaaa
    10921 cttttgagaa ttttggtcta ttcggactaa gcgccttgac ttctataggg gcactagata
    10981 ttaatgagcg atttatgctg gaatctatat ctttggaatt gcttaaacga cagttagttt
    11041 cgattgataa aatgacttca ttagatggta tcaaaattgg gttattgaat tcaagtgaag
    11101 caatcatggt tgttcgagac ttcataaaaa caaaacaaaa tatcccaatc attttagacc
    11161 ctgttttggc attcaaagaa actgaagctg tgatcaatca attgtatatt tcgcagctca
    11221 tcgaagaact ttttccactt gttacgatga ttacgcctaa tttggcagaa gctgctttac
    11281 tttctggtca agctcttccc tcttcgttaa cagaaatgat ccatctcgct aaagaactca
    11341 ctgcttcagg aacaaaaaac attgtgatca aaggcggcac aaggatggct ggagatgaag
    11401 ctgtggatct tctctattca attgatcatt atgaggtatt ttctcataaa aaaatcgaca
    11461 ctacaacagt aaacggggcc ggttgtgcct tttcttctgc aataacagcg aatcttgtgt
    11521 taggggcaga ttttaccaaa gcagtagcag aaagtaaaaa atttgttgcc cattgtattt
    11581 ctaatggcgt gctgatgaac gatcataccg gaagtgtctg gtctggcggt attcaaaaag
    11641 gaggaatgag aaatgaaaac taagcaatta acgatctatg ctatgctgat tgcgttgaca
    11701 gttgcccttt cattaacaat tttgatccct gtaccggcta cgaatggatt tgtgacatta
    11761 tgcgaggccg ggatctatac cgctgctttt ctttatggtc caacgggtgg attagttgtt
    11821 ggtgcttcca gtggtctatt gatcgatttg atttcaggat atccgcagtg ggcagttttt
    11881 tcattagtga tccacgggtt acaaggatat gttgctggtt ctttcagcaa atcttctact
    11941 cgtatgtggg gattagggct tttattagga agcttagtta tgatcatcgg ctatggtatc
    12001 gccggttgga ttttatatga ttggccggct ggagtggctt ctataccagg aaacatcttc
    12061 caaaatctga ttggaatggg acttgccttt cctataactg caagtcttca acgtattcaa
    12121 gcacggaagc aatattaatt gaaaaagaag gttttagaaa atgaagttgt cagaaaaaga
    12181 attaaaagac caattgactg aaatagttaa tgacatattg gctgaggcac acttgaaaaa
    12241 aggtgatctt tttgttttag gatgcagcac aagcgaagtc gttggaggac atattggaaa
    12301 aaattccagt gcggaagttg gtcaatggat catccgtacg ttaaaagagt tgttggatcc
    12361 taaagaaatt gcgctagctg tccaaggttg tgaacatttg aaccgagcat tagtagtaga
    12421 acgaacagtg gcagaagcaa aaaatttcga aattgtctct gttgttcctg cactacatgc
    12481 tggaggtgct tgttctgttg ctgcttttga ccagttcaat gatcctgtcg aagtggaaca
    12541 cgtagttgga caagccggta tcgatatcgg tgacacatca atcggtatgc atatcaagca
    12601 tgtccaagta cctgtcagac ctcgtttgaa aacccttgga caagctcacg tgacagccct
    12661 tcgctctcgt cctaaatata tcggtggtcc tagagcgaat tattgaaatg agtaattgac
    12721 agagcaatct gaaaaacttc ctcgcaaagt ccaactaatg aactttaacg aggaagtttt
    12781 acttgtttgt tttatataac cgagcttcat aaggtgctag tatgccttta gcttcaaatt
    12841 ctccgctaga ggaatttgac aagagtaatg tccaggactg atctgcttct tttggaagtt
    12901 catagtcagc aagtgattct gttaagttta ccataataag atattgttca tcttctaaat
    12961 atctttcata gacataaagt tgtggatgtt catgaagata ttctttataa gaaccgtata
    13021 tcagaccttt attggtttgt ctaatgcgga tcatgttctt atagaattgc aggaccgact
    13081 ctggttcata ttcttgttgt tctacattga tcgttttggt atttggatta gtgatcagcc
    13141 aaggttcatg tgtagaaaaa ccagcatatt gttccgaagt ccattgcatg ggtgtcctgc
    13201 tgttgtctct cgttgtttca cgaatgatat ccaatgcttc ttctcttgtc ttaccttccg
    13261 caagcagcct tttataaagt cttttcgaat ctactgcatc aacttgatcg atcgattcaa
    13321 agggcatatt cgtcattccg atttcctgtc cttgataaat aaatggcgta ccttgcaaaa
    13381 agaaatacat catagcgatt gcttttgcag acatagccca aaaatcggga cgagtatctc
    13441 caaacactga aacggcacga ggaacatcat ggttttccat atataaagcg ttccatccga
    13501 taccatccaa tgaagtttgc caagcgctta atgcatgttt caatttaagg acatctaatt
    13561 gcccttcttt ttcttgctgc cagatatgga tatgatcaaa ttcgaatatc atatcgaaat
    13621 aaccgtcttt ccctacccat tgtggtcctt cttctgcagt gacaccactt gcttctccga
    13681 cagtcatgat atcgtactct tcaaaaacat gtttcaaatc ggtcaaatgt tcttcgattc
    13741 ctgagacatt ttgaaagggt gagaaagggt tctctgtggc ttttactgaa tattcatctt
    13801 tttttacatg agaaatcgca tctaaacgaa atccatctat ccctttatcc aaccaccatc
    13861 ggatcatatt gaatagttct tctttcaatt tctcgctttc ccaatttaag tctggttgtt
    13921 cttttgcaaa aacatgcaga taatactgtc cagtttcatg agaatactcc caagcagaac
    13981 cgccgaaaat cgatgtccaa ttattcggtt ctttgccatc taccccgtcg acccaaatgt
    14041 aataatcgcg atacgggctt tctttagatt ttttcgattc ttcaaaccat tgatgctgat
    14101 ccgaactatg gttgatgaca agatccataa tcactttgat ccctaattca tgtgcttctt
    14161 ttagcaataa atcaaaatct tccatcgttc cgaatctctc ttctatatct tgatagtcac
    14221 ttacgtcata accattatcg acaaatggag attgatagat cggattgatc cagataaagc
    14281 cgataccaag atctttcaaa taattaagtt tttcacggat cccattgatg tctccttgac
    14341 catcgccatt gctatctttg aaactagcag gatagatttg atagcctact gcttctttcc
    14401 accatttttg ttcttgtatc atatcgtttc actcctttaa cggtccctct tgattataac
    14461 cgattctatt ttagaagtat ccttgttacc ggtaactaga aacgaaaagc tcaacgaatc
    14521 tgctaagtgc gttcgttgag ctttatttta tcatagatag cgggcaatca gttgaatcat
    14581 ttgcttggga aattcattca agtcagtaat atctacaaaa cgactttcac cgtagatcga
    14641 cttgatttct tctttgtctt ggccaatagc tgcggagaca aataggattc cttgacgttc
    14701 atattctttc aaaacagact ggatatcttc ttttgctttt ttgcctgtat aatccggcaa
    14761 cgcttttggc tgaccgtcac taatattaag caacagtttg gttgttgcgg attgtctgtt
    14821 taatttttct gctgctaaac gaagtgcagc accatcacga ttgttttgcc ttggtttcat
    14881 agtgactaat ttttcattta accattgaaa tccgtcttca aattctttat aagaaaacaa
    14941 agaagtcttt tctcttgacg aacggtctgc tgtatctccg taaatcaaca atgggatatc
    15001 gactttctta gaaaattcgg caatggcaat cgctgctttt tttgccgctt cgatccgatc
    15061 atcccgtatc attgacccag attcatcgat ccgtacagct accgctaaag aaggttgctc
    15121 atgtggcggg ttctttttgt caaaatttct caaatcacca tatgctactt tgcttgcatt
    15181 gaaccgctga ccttcgtatt ttcctttttg gtacgtctct gtttgttcat tatccagtaa
    15241 ctctaaaatt tgccggctaa gtgattctac tattggcatt acttgcatgt gtagctcacg
    15301 tgcttccggc gtttttcctt ggaaagctgg tcggtgaacg atgagacctt ctttttcatg
    15361 aatcgtttcc ttcatgattt gtcgcgcttc tttattcagt tgcttcttga tttccttatc
    15421 atgtgcttcc atgtcagaag cagacatttc ttgagaatca tctcctgttt gttgttcttt
    15481 ttgatttttt ccttcgatat cgctactgcg ttctttttgc atttgaggag gaatctcgtt
    15541 tttttctatt gtttcttctt gtttctcttc tccctcattt ggttcttcag cagacaacga
    15601 tttcttttca tctaagcgca gaatttcttc agcattttca gtgatgtttc gttcatttgc
    15661 ttcttcggcg ctaatggttc tttcttttgt atgctcagat tgaggatgac cccatttttc
    15721 ttttctcaat atgtcctgaa gttccagtag caatccactg tcagacaaag cttgttcaag
    15781 aattgccaat tcatctgaat cagtggataa tttatacaaa acttttggat aaatcgactg
    15841 gagtaaatct tgtccttgct tataggtatt tgcccaataa aaataagaac gcattcctgc
    15901 tacacctttg atggcgttag cttttgcagt ttcatccaac aaacggataa tctctgccat
    15961 ctttaagaga agttcttgtt tttggatcat agttttcgcc atcgcacgtt ctgccatgac
    16021 ttctaaggca ggaagatcca ttttttccgc atgttgcatc cgatctctta aagattcatt
    16081 taatgggcga tttccttggt aatttcgatt tgtagtgatc acaatgacgc aatctggatg
    16141 gcgacgaatg attcctgtcg gtaaattcaa catcccattt ggttcgagcg ctgagttaag
    16201 tgccacaaga acagaggcat ctcggatgac agtcggctct tgaatctcta ataaatagcc
    16261 tttttcaatc gctcgaacga tttcagaagg gtaatagctg taatggattg catctggata
    16321 atcagcttcg atcctttgaa ctagctctgc taatttttct ttagcagaaa aaggatcaat
    16381 gccgtaatgg ttctggatca gtgtcagtac agcttcaagt gtatctgttt ggtaaatagc
    16441 ttctagtaat tcttggtctt gttccttatt agaatctact acaggtaata aggctccgaa
    16501 aacatctgat ttgtccatat ctgcaaaaca ggtcactttt gtatagggaa gttgcagatc
    16561 agcagatagt gctttggcta attgtgtctt ccccgacccg gcatctcctt ccaaaagtac
    16621 gttactgatt ttcatttcag gatcttgcca attccctttg atttcggagg cgatccgcca
    16681 ttcttctgca cttgtccgat ggcttttcgg tttcttccag atcatttgct tttctagccg
    16741 tgaaaaggtt cggttttctg ttagtgagaa attctccatg cttctcttcc ccttttgatc
    16801 atctctgtct attaatatcc tagctcatct aaaagacgtt ttaaaatacg gaatcgttcc
    16861 ccaatcagtt ctccgtcttt ttgtagtgta tcgtaataca aaatgatgtc ttcatcgatc
    16921 tttggatttt tggtatctac ttctaccgtc attcggttcc cttgtaatcc aatgatgtct
    16981 acgactggat tttctggatc ataaaatttt tcataacgat aagcatcttg gaagtataaa
    17041 cttccttgcg cagctaagat cgctaaatct aaaatacgtg tgattggtaa ttcttccgac
    17101 tgtcgtgacc atttttctcc agtatgtcgc cataccttgc ctgagatatc gacttttcca
    17161 cgatcattcc actgagcaag gcccagtgaa agcccttttg catccatatt taatgcattt
    17221 cttccatcaa ctttatcata gtcttctaca acgattactg gtttatgttt taaatttgtt
    17281 ggtatttcca ttattttaac tcctcatcct tttattttta gtaaataagt aaattagtag
    17341 tttactcatt tactaaacta tatgcgacat tatagaggat tgaagtgagg attgcaaaga
    17401 aaaagagctt cattgataga aataaaaaca ccatctcgtt cttttacacg ctcattagta
    17461 acatgtagaa agtccaagat gatgttggaa tattttggct ctatactttt tgatgttggt
    17521 gaacttcacc aacatctttt ttg
//
LOCUS       DAZKOK010000057        17527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000057 DAZKOK010000000
VERSION     DAZKOK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.057"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..628)
                     /locus_tag="ACG90W_001636"
     CDS             complement(<1..628)
                     /locus_tag="ACG90W_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0477996.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFAL"
     gene            complement(777..1490)
                     /locus_tag="ACG90W_001637"
     CDS             complement(777..1490)
                     /locus_tag="ACG90W_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0477997.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            1665..3089
                     /locus_tag="ACG90W_001638"
     CDS             1665..3089
                     /locus_tag="ACG90W_001638"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0477998.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            3388..4095
                     /locus_tag="ACG90W_001639"
     CDS             3388..4095
                     /locus_tag="ACG90W_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0477999.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4082..4405
                     /locus_tag="ACG90W_001640"
     CDS             4082..4405
                     /locus_tag="ACG90W_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0478000.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(4494..5531)
                     /locus_tag="ACG90W_001641"
     CDS             complement(4494..5531)
                     /locus_tag="ACG90W_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0478001.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5852..6835)
                     /locus_tag="ACG90W_001642"
     CDS             complement(5852..6835)
                     /locus_tag="ACG90W_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0478002.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(7250..8020)
                     /locus_tag="ACG90W_001643"
     CDS             complement(7250..8020)
                     /locus_tag="ACG90W_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478003.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8013..8948)
                     /locus_tag="ACG90W_001644"
     CDS             complement(8013..8948)
                     /locus_tag="ACG90W_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478004.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(9264..10913)
                     /locus_tag="ACG90W_001645"
     CDS             complement(9264..10913)
                     /locus_tag="ACG90W_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0478005.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11030..13633)
                     /locus_tag="ACG90W_001646"
     CDS             complement(11030..13633)
                     /locus_tag="ACG90W_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0478006.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(13645..14694)
                     /locus_tag="ACG90W_001647"
     CDS             complement(13645..14694)
                     /locus_tag="ACG90W_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0478007.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(14720..15034)
                     /locus_tag="ACG90W_001648"
     CDS             complement(14720..15034)
                     /locus_tag="ACG90W_001648"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0478008.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15050..15496)
                     /locus_tag="ACG90W_001649"
     CDS             complement(15050..15496)
                     /locus_tag="ACG90W_001649"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0478009.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(15497..17419)
                     /locus_tag="ACG90W_001650"
     CDS             complement(15497..17419)
                     /locus_tag="ACG90W_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0478010.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         5235 a         3551 c         2864 g         5877 t
ORIGIN      
        1 ataaagcaaa cgaggtaatc gtaataatga ttgggattaa tacattaaaa gactttaaaa
       61 cagcgggagg aatatttccg gatagctgaa tctgtagttt cttattatta gaaagtttaa
      121 taaataattc tgtagccgct aatccaacaa atatacctac aaacattcct gaagctcctg
      181 tataggatat aggtatcaca cccgtcactt caacagcttt atccgcattt tcaggagtaa
      241 acatcatctg cataggtgtt aatacaacaa atgtcgaaat agcaactaat accgctgcaa
      301 tatgatttgt ataatttctg cttgcacaca tatgataaga tactgctgct acaactaata
      361 atgtagttat gcttaatgta ccattggcaa ttgaagtacc aatttgttgt aacgtggtca
      421 atgtactagt atcgacaatt tcccccatga atcctgttgg ttctaagatc actgaattaa
      481 taagtgtcac aaatcctgct aacatcataa atggcataaa tgtagcaaac gcatctcgca
      541 aagttcttaa atggatctga ttccccaact tagtcgcaac tataccaaat ttttcaaata
      601 caatgtcctt cctgctactt ttgcccatat tttttctcct ttatgcttat tgtaatcgat
      661 tactttttcc acccaaataa tacatcactt atcaaaaaaa gtaaatactt ttaattaaat
      721 atatatatct tttttagtat aaaaaaataa gctctgaaaa aataattttc ccgagcttat
      781 ttttcaacaa aaacagcttc aaaaacaaag tcttgatata aatgtctcga taccgaatac
      841 tcgaaaggtt taccagacga tagagcaaca actttgtcta tctcaatcat aaattcacct
      901 ggatctattt ttaatatttt tgcgtcaaat tcattcgcag tatctccttt gatacgtatg
      961 tgcgaagtct ttatttccag acccagttca tttgtgatat aagcatatac agatttcttt
     1021 aaatgttttg gttccaagcc tggtatgact gctaatggca tgaatgtttg ctcaatcgag
     1081 tatggttgac aatcaataaa acggcttcgt acaatattat atacatagtc ttttacactg
     1141 atacttaatt tatcagctac ttttttcgat ggaagttcaa ttgagaaagt atggatttct
     1201 gaatctacgt gttcttcacc aaccacgcta aacaaacctt cgtttggacc aagaactcgt
     1261 gagttatcaa ttggtgttct gacgacttca gtcccatacc cacttctact tcgcaaaact
     1321 ccttcggaaa caagtaagtc tacgcctttc tttactgtta acttgcttgt atgataaata
     1381 actgccaatt catctcctgt aggaagtttc tcaccaattt tatactctcc atttacaatt
     1441 tttttcttta tatctaagta aatttccata tacttcgcaa tttttttcaa tggtgaccct
     1501 ccaatcaaaa acatatcttc atctatactt taacataaat aaaccgataa aatccacatc
     1561 aaaattatac acacaataaa aaagatatat atatttaaac aaaactattt actttaagtt
     1621 gtaagcggtg ttatcattaa gtggaaacag gaggtaaaaa aattatgaca actatgaaaa
     1681 agctaacttt tccagatgat ttttggtggg gttccgcttg gtcagctgag caagcagaag
     1741 gaaaaggaga aacaggaaaa gcagagacta tctgggatcg ctggtttaaa gaacaacctt
     1801 accggtttta tcaacgaatc gattcctcta ttacaaccga tcacatccat caatataaag
     1861 aagatataga actaatgaaa aaaactgggc ataactcctt tcgcgtttct atttcttggg
     1921 cacgaatgtt tccaaatggc ggtaccggaa aagtaaatcc caaagccatt tctttctatc
     1981 gaaaattatt ttccgaaatg aataataacg gaatcaaagt atttgccaac ttatatcatt
     2041 ttgacatgcc tgctactctt caagataaag gaggatggga atcacgtgaa gtagttgatg
     2101 cttatgttca ttacgctgaa acttgtttta aagaatttgg tgacttagtt tgtcattggt
     2161 ttaccttcaa tgaaccactt gggcctattt taggtactta tcttgaagat tttcattatc
     2221 caaacctcat tgactttaaa cgcggagcgc aagcagcatt cttcactata cttgcacacg
     2281 caaaagcaat acaagtattt aaagaactag ctcttcctag taaaataggc gttattctaa
     2341 atttgagtcc tacttttcct cgtagtcaaa atcctgctga tgtgaaagca gcagaaatcg
     2401 ctgatttatt ttatacgcgg agctttttag atcctatggt taaagggaat ttttctaaaa
     2461 agcttatcct gttattacaa gaatataatc agcttcctga agattatacg aaagaagatc
     2521 ttgtatgtat ctacgaaaat accgctcaaa ttcttggact aaactattac gagccacgtc
     2581 gcgtcaaatc tcgttcttct atggtggata catctagtcc atttttacca gagtggtttt
     2641 tcgaaccaca tatcatgcct ggacgacgaa tgaacgaata ccggggatgg gaaatttacg
     2701 aaaaaggaat ttatgatttg tgcatggata tcaagaacca ttatggaaac atcgaagcat
     2761 ttatctcgga gaacggaatg ggcgttgcgg atgaagaacg tttcatggat gatacagggc
     2821 aaataattga tgattatcgg attgagtata tcaaagatca tcttgcttac ttatggaagg
     2881 caataaatga aggatgtaac attaaggggt atcatctttg gacatttatt gattgctggt
     2941 catggatcaa cgcctataaa aaccgatatg ggctggtttc actcgacctt actactcaaa
     3001 aaagaacaat taaaaaaagt ggcgaatttt acaagcaatt aagtgataca aatggattta
     3061 gatacgatgt agacttgtta gttgaataat attctaaaat tccacttgaa gtgaacacat
     3121 tagaaaacaa gaataatgag aaataccaat aacaaatact ttttcttcat acttcattta
     3181 tacttgtcag tataagagac tgtgacaaat gtagtgatcc tcagaagtta ggtctggaaa
     3241 tctaacttga gaagattttt ttaaactacg tccagtcttt tttatttgtt taaaattgaa
     3301 gttgacaagt cttcattaaa aatttagaat attcacaagt ttgacgacaa ttatgaaaat
     3361 ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa aactgcgatc
     3421 aaagacacgt tacctaccgt ttttggttat atcggtattg gacttgcatt tggtatcgtt
     3481 gggaaagctg ccgggtttca tccgctggtc gtcacgttga tgtccctgct gatctatgct
     3541 ggttctgccc aatttatcac ggtcagcatg cttgctagcc atagtccact gctctccatc
     3601 gttctctcga cctttctagt caattcccga atgattctga tgagtatgac gattgctcct
     3661 tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct gacagatgaa
     3721 agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa ctttcgttgg
     3781 atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct tgttggcgca
     3841 cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc catcgtcgct
     3901 atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc taaacgatta
     3961 cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat ttttatccca
     4021 agtaacttag tgattttagt tgtaacactg attgggtgcg gattgggggt gtggatcaaa
     4081 catgccttct tttgagttta cattgctgac tattttaggt tgtactttag caacttggct
     4141 ttctaggatc ctaccatttg tcttactgaa aaaattcgat ttgccgcaac cgttgcttga
     4201 gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca gtctatttac
     4261 ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt cagttcctac
     4321 acttttagct gctattcttt caaaaagctt attggtgatc gtcttggtag gaattctttc
     4381 cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa attactgtca
     4441 tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct tgcttatccg
     4501 ttgaacggac cactaacaac gacttttcct aatatatgtc ctgtttctac gtctttggtt
     4561 gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa tgtactgcgg
     4621 ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg cgtttcgatt
     4681 tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat agattggttt
     4741 ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac atgtccaaat
     4801 ggtcggacaa ggtgtttgat ttgatcaaag tagcgggtaa tatcgaataa tacagcgaca
     4861 tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg atgataatcg
     4921 attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact tgcagtcgcg
     4981 tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac gcccccagct
     5041 ccgttgatca ctaagatttt tttcccgcgg ttggcatttt cttctggagt cagaccaaat
     5101 ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc ggcttcttca
     5161 tctgaaagat tttttggtgc aagagcgaca atacgttcgt caaccagttg gtattcttga
     5221 ttgcttcctg ctcttgtcgt tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt
     5281 tgcacttcat cacctacagc aacaactttc ccgactccat caaaccctaa gatacgtaaa
     5341 gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac agatactgcg
     5401 ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt ttcatcaata
     5461 aagctttttg gatcatcgat cgacaaccct tcataaaaac cgactacctt cattaattcc
     5521 ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat acagttttca
     5581 tttcaatata tatagtatgc tatttgttcc acttttattc atcataatta ttctcttact
     5641 taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga cttttgtcac
     5701 aacctctttt aaacggtgtt caatcaactg accctcggta ttcgctcaaa aaacgacaaa
     5761 atatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac
     5821 gacctctaat tcacggtgtt caagcaagaa atcattgttc actgaaaagc gttggatgtt
     5881 ctttttcaaa ggtgtctaag atctctcgta cttcatcagt agatttcgta ttcatcaact
     5941 gaactcgcaa gtcatttgct cctggaaatc ctttgatata gattttaaag aaacgatgta
     6001 gtcctgtgat ggaacgcggg atgatttctg aataatgatc ttgcagatct agttgcagac
     6061 gaagtagtcc gatcagttct ttttctgtat gttccctcgg ttctttttca aatgcataag
     6121 gattcttgaa aatcccccgg ccaatcatga tgccatctac gccatatttt tcggccaatt
     6181 cttctcctgt ctggcgatct gggatatctc cgttgatcgt gatcagtgtt tgcggagcga
     6241 tttggtcacg taaagcaatg attttaggga tgacttccca atgtgcatct actttactca
     6301 tctctttacg cgtacgcaaa tggacagaaa gattggctat atcttgatga agtaggtgac
     6361 tgatccattc ttccatctca gacatttccg tatagccgat acgcgtcttg acgctgacag
     6421 gcaaaccgcc agctttagcc gcttcaatca attgtgccgc tacttctggg cgaaggatca
     6481 agccactgcc ttttccacgt tctgctacat tagggactgg gcagcccatg ttgatatcga
     6541 ttccttggaa gcccatttct gccatagcaa tgctcatttc tctaaaaaat tctggcttgt
     6601 ctccccaaat atgggcaacc atcggctgtt catcttctgt aaaggtcaat cttcctcgta
     6661 cactgtcttt tccatctgga tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga
     6721 catccggtgc tgctgcatgc tttacgacat ggcgaaatac aacatctgtt acatcttcca
     6781 ttggtgctaa aacaaaaaaa ggctttggca gctctttcca aaaattagta gtcatttatt
     6841 tgttactctc cattctatca agcagtgaag tcgcaatctt tctcaaaaac attgtcatta
     6901 tactaggaaa caaaaaaaga agcaaagtga acagcttaaa agatgtttct gattgagcaa
     6961 aaaaagactg tgacaaaaat cttatcacaa ttccttttcc ggaataaatg gtgttcaaaa
     7021 gcttgcatct gcggtaataa gtccaaccaa acgaaaaata ggaggagcta tttttgtttg
     7081 gctgttctta ttacttgatg cagaacagct ttttcacaac ctctccacta aacggtgttc
     7141 aatcagctga ccctcggtat tagctcaaca aacgacaaaa tatgaggaac tattttcccg
     7201 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttc taacttctca
     7261 atcctcttcc tttattgatc aaataccaag taacggcgta caaaacaacg atgaatagca
     7321 caagtacgat catcgatgtt gctactggca catcgattgt tccaaggaat ccgtaacgga
     7381 aaccggagat catataaacg atcggattga ctttcgaaac agcttgccaa aatggcggca
     7441 acatagagat ggcatagaaa acgccaccta gataagttaa cggctgcaag acaaatgtag
     7501 gcacgatcga tacatcgtca taagactgag caaaaatccc gttgagcagt cctgctaacg
     7561 aaaacaagat tgccgtcatc aataatgtaa tgatgaccat gaaccatgaa tacacatgaa
     7621 gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc gacgagaata ctgcgaccca
     7681 ctccgccaat cacaaagccc caaatgatca catgcgtagg aacaggcgct accaagattt
     7741 cttcgatgtt tttctggaat ttttgagaga agaaggacga agaaacgttg gcatacgaac
     7801 tagtgatagc agacatcata atcaaaccag gcacgatgaa ttccatatat gaaaaaccac
     7861 ccatgtcacc gatccgacca ccgatcattt ttccgaaaat aacaaagtac aaggatgtcg
     7921 tgatcacagg cggaaccagt gtttggaccc agatacgcaa atagcggttc gtttccttga
     7981 tagctaaact ttttaatgca gtaaaataaa gattaaacat ttttctcctc cgtatgatta
     8041 ttttcttctg tgattttcaa aaacagttct tctaaacgat tcgacttatt cctcatagaa
     8101 agaactttga ttccttgtct tgtcagctga tcgaagattc cgttcacccc ttgatttcgt
     8161 tcaacttcga cagcgattgt ctgttcatct tcaaatgtac aagcgtatcc ttcaatcaca
     8221 ggtttggtct catatggcgc taaatcaaag ataaatgttt caaattgcag tttggataga
     8281 agctctttca tactagtatt ctcgatcagt tcacctgatt ggatgatccc gatgttgcga
     8341 caaagcattt cagcttcttc taaataatgc gtcgtcaaaa tgatcgttgt accattttcg
     8401 ttcaattctc ggagaaactc ccacatctcg cgtctcaatt caatatcgac tcctgctgtt
     8461 ggttcatcca agatcagcaa acgtggttca tgcatcaatg ccctagcaat catcagtctt
     8521 cgcttcattc ccccggaaag catacgtgca cggacatttc tcttttccca aagattggac
     8581 tgcttcaaat atttttcgct gcgtttcatc gcttctttgc gagagacacc gtagtagcct
     8641 gcttgattca cgacgatttg ctggaccgtt tcaaagggat tgaaattgaa ctcttgcggg
     8701 actaatccaa tctgttgctt tgctcggact agatccgtat ccagatcata accgaagaca
     8761 ttcacttttc cagatgtttt attgactaaa gaagtaatga ttccgatcgt tgtagacttc
     8821 ccagctccat ttggtcctaa tagcgcatag aaatctcctt ctttcacgga aagatcgatt
     8881 cctcgcaatg cttcgacccc acttccataa acctttttca aattatttat ttcaagcgca
     8941 tttgccattt atttcctatc tccttataga catgattctt tattgtatca tggaatacag
     9001 cagatgaaaa aacaacttgg ttccagcgaa aaatcaggtt gctgggctca ggaaaatctc
     9061 ctaatgaaag acatcaatcg gacgaagtca gacaaccttt tcagaaaaaa caaatcgaag
     9121 ataaaggatt actattcata tgtgcaacaa aaggagcgtg agacaaaagt aaagaacact
     9181 tttgtctcac gctctaaaca cgtataaacg gcggaagcaa aagccaagat ttttgatcca
     9241 ataaatactt tcgatcactt cgcctatccc aatacatgcc actgccgctt ttcatcaaat
     9301 aacgcggctt ccgtttttgc ggtttctact aaagtctctg ggtaatccaa aactttcagc
     9361 aaggcaatcg catttcgggt ccttgaaggt ccttctttta atttgaaatc aaaactgatt
     9421 ccttttcctt ctgttacttg ttcttcaaag tggacattct cacaataatt ctttaaaatc
     9481 tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag atggatactc cgcaagccac
     9541 cgaacgactg aagcggaaga agcaatccgt tcgatcgtat ttgtcccttt taaaatctca
     9601 tctacgaagc agtagcagcg ttctttggaa gccacttggt ccaacaaacg tttgatcgat
     9661 ttgatttctg aaacaaaata actgtcgcct tcaaataaat catcttcgat tgccatggaa
     9721 gtcaacacat gtccaggttg cagggtaaac tgttctgcaa tcgctgtttg gatcgtctgt
     9781 gctagaatac agctgatggc tatacttttg acatacgtag attttcctga tgcattggaa
     9841 cccgtgacta atgtattctt ctgccagttg accggattca ctaccgcttc ttttaacaat
     9901 ggatgataga tatctttcgc tgtcacaccg ccctcagtaa actctggtcg gcaagtgatg
     9961 ggcatataag tacggtaatt caatattgca gcggctactt ctaattctcc cagcaattcc
    10021 cacagcttga tggcttcagt tgaatgcttt tctaatttag tgagaacgaa atgataggag
    10081 ataaatggca gcatgaacat gatgttgaca tattcaaaaa tcaactctgc ttcactccca
    10141 ctcttcatac gaaatgaaaa acctaattta gggatgtctc ttaatggttt agtttgctgt
    10201 ttcagctctt cttgaagtgg tgtcggcagt tttgctagct gattggcggc tgaaatcgtt
    10261 gaaaccaaat aactcatgct attcagctct gtttctagtg tttgtttctt cactagataa
    10321 tagaccgtat tgaatactaa cccaccgatt gccaaataga ttcctagtac ttgtcctaaa
    10381 agaagcaaac ccaaacccac cagcggaagc aagccaagaa aaataaataa acctacgcgt
    10441 ccaatctttt tactttttcc atccgctaga tattgttttg caaaattatg atcttttttt
    10501 cctaaacgag cgaattgata ttggattttt tctctcagtt gaggattttc ctgataaaaa
    10561 gcaatcagtt tttctaactg ctgatcttca ccaaaatcaa agctgcgtaa acgttgatac
    10621 aacgcttctg aacctacact agaataggta ctgttgattc cttcgaatac ttcgaacata
    10681 tccaggtcat accaagtaag atcatcgatt tcgctatccc atgaacggaa cttcttctcc
    10741 gtgagccatg cttccttcaa gctttcttct ttatcaaatc tcggttgata aggaattttt
    10801 tcccatttgc tacgaactgt ttcctttaat ttcaaacgtg aaaaaatgtc taaagcaact
    10861 aataaaataa cagctgcaat aataattaaa acgataaatt ctctttgacc catatccctc
    10921 tccgttcctt tatttgttag ctttagtata gcactgttgc gctatttgaa aagagggtaa
    10981 aaaaacacct ttcaaaataa aaggtgtttt tttcttgtat attacatatt tatctctcat
    11041 ttatcaaaat tgacaaataa tcatagggag gaattgttgt taattcttgt ttattctcca
    11101 gtgcattgac aactgtccca agggaaagga tagcctcagg tactattttt tcttgttctg
    11161 ttggattagc taagcgaaga acaaatcctt gttcatgaaa ataagaagga taaatcgatg
    11221 atacaattaa gtcttcaggt aattcaatca atgaaaattt acgaggaaca tcaagttcat
    11281 tttgtaatgg ccaaatcttg ttatctaacc tgtgaataaa tacattcagt ttctgctttt
    11341 gataagaaat agattgttct aaccgttttt ctgcctgttt tgccactttg aactcattaa
    11401 atcttccttc ttctacatta aatccaaaag tgactttata ctcacctatc tcttgagcta
    11461 gcggtgtttc catcatgaca tgcccttcat ttgtcgtatc tcctgaagct cgcccaggtc
    11521 gccaagcaag attaggtttt ccaagttccc ctgttgttga aaataaggtt atgattaatt
    11581 gattttctat acgttcatac tcttttaaac catctgcata aaacgtaaaa taatgatcat
    11641 tttctgccac agagacactc ttatcgaata tttctaaatt aacaggtttc tctaccatag
    11701 tttctgacca attcggagga atgacatcag gggtattttc aataaatccg ttttgaattt
    11761 gtgcaattga tttttcagat ttatctaaaa cattaattgc taagcgcata cgatgcgagt
    11821 aaacagtatt atgaattttg agtgtgcctt caacgatttg actttctccc agactgaacg
    11881 atatttcata agatatttct tccatttcgg gtttttcaga taaacgatct tttaaatctt
    11941 taggaagttg agcttttccg taaacaacta atgtttcttg taaagaatct ttatatactt
    12001 tagctgtttc taattttaga gatagttctg tatctccttc taatggtgaa taatcatacg
    12061 tatcgccatc atttgctgaa tcgatcaaat gaacgaaatc aagatattgt ctatctccaa
    12121 ctatcaaatt caattgccca tctttaaaag aaagtttata tttattgtta cttatctcag
    12181 ttttgtctat ctcgattgct gtttctaatt tttcattatt tttctcaaat tcaacaacag
    12241 tgtatcctag tgctggcaca tttacattta tttttatgtc aagttcataa taagctggtt
    12301 catcaaaata ttcgaaacct gtagcaatca tccgataagc atttttccgt tctggaaaat
    12361 aaacttcttt ttcaatgata ttatagggac tacctttgaa gtgtattttc tttgaatcag
    12421 ctactacatg cacgattttc gatccactat aattctcagt cgttgtgttg aaaacaagga
    12481 cttcattcga ttttagcttt aatgcatcag ccatccgttt tacaataagg ttttcgattc
    12541 cgtcagctat ctcattagct tctttgattc gatggaaaat atcttccgca acattatctg
    12601 aaacacagcc accaatacta tcatgagcct ggttttgcaa aatctttttc cataaccgac
    12661 gaagtaggcc tttactgata tggataccat ttctttctgc aatcagcatg agcggctcaa
    12721 ctcgttttag aattttttgt tccaatttaa aattttctat tttcattttt gttcttactg
    12781 agccaataga acgatgaaca cgagagtaaa ccggctgacg aagttcacct ttatattcag
    12841 gtaattcttt tttgtttctg ataacttcta cataagaagg ataatctgaa ataatattgt
    12901 ggaatttact gtatttattc aacttctcta cgacttgatc aaaatttaaa atcattgact
    12961 tttgatctac acccgaaggt actaaaatat ctttgttttt tccttgcata tgaagcattt
    13021 caactgcttc atccaacttt tcttttgcaa attcaggaat atctttaatt gcgtctaagg
    13081 tcatcattcc agtcatataa ccaaatggaa aattcatggc ataaacaaat ttatctccca
    13141 atcctttcca gataaaataa ggagatggaa ccatcgtttc aaagtttaat cctcgccaac
    13201 acatgaaatt atcgatttct acttgatgca aaagtgtagg caaattcgca ttgaacccaa
    13261 acgtgtcagg caaataccct accatcattg gatctccata tttaaggatc gtatcactga
    13321 ttccaatcat taagtttctt aaaattgatt cagcatccac taataaggca tctgtttgtg
    13381 cataccacgg tcccacgaat agttgtcctg ttttcatcag tcgttggata accttaagct
    13441 tttgcggatt gatctccaaa tactcatcga cgattgaagt ttgtccatct aaacaaaagt
    13501 ttactttagg gttcctttcc aattcatcta gaacttcagt aaagacctga tcagacaata
    13561 cgatggcatc ttcatttgta aaataccatt ctctgtccca gtgcgtgtgt tgaattatat
    13621 gtgcatttac catatcagat tctcctactt tccttcttct actgaattta cttgttgttc
    13681 ttttttatac ttgatggatt ttagtgcatt gaccattaac gctgtaacca ctcccccaat
    13741 aacaattgat actaaatata aaggtatatt cgtaacaaaa ggaattgcca taaaggttga
    13801 aagcattgtt tcttgagtaa caccaaaaaa tccatttaga ccacatgcta ctgcagtgcc
    13861 caccacgatg cttggtatta ctcgtaaagg atctttcacg gcaaacggga tcgtcccttc
    13921 agtaatcccc acaaggccca taatcatagc tgtctttgca ttatctcgtt ccgattcatc
    13981 ataaagtttt ttgtttaaaa ttgttgctaa aaacattcca aatggcggaa tagcaattcc
    14041 taataacatg gctgtactag gtccaaagct tccgcttgaa aaagaagcaa ccccaaaagc
    14101 atatgcgatt ttattgatag gtcctcccat atcaaacgcc atcattcctc ctaaaatagc
    14161 cgacatcaaa gcagcatttg ctcctgacat gccttctagc caattagtca gtcctgttgt
    14221 cattgcttga ataggcgtag ctagtacata ccataatatg attccaacaa ctaatgtagt
    14281 aacaaaagga atcagcataa ttgattttag actaataact tgcatcggta cttttacttt
    14341 tttcaatgca ttagctaaat agccacaagc taatccagca accattcctc caataaatcc
    14401 cataccaaga ttttgacaca tcatcccagt gataaaagct ggtgctagac cgggacggtc
    14461 agcaattgaa tatgcgatat aggctgaaat catcggtacg ataaactgta atcccgtctg
    14521 accaatcatt cgcaggactc cccagaatgt atccgtctgg tcccaaacac ctgtgccacc
    14581 aaaaatgact gcaatagaag ttagtaaccc tgctgctacg acaagcggga tcatatagga
    14641 aaccccactt tggaaatgat ttagaatttc tcttccttgt ttcttgaatt ccattagtaa
    14701 ctcccccacg atatattttt tatttgttaa ttaatgcaat tgcttcatca atcgtctctt
    14761 caccatcttt tactacctct gaggtcccta ctgttaaaat cggtttaccg tcaaagcgac
    14821 ctcgttctga aacatttgta tctactgcaa atatgacaac atctgcctcg gaaacatccg
    14881 aaattgaaag tttgttttct atccccatag ccccctgtgt ttctacttta atatctaacc
    14941 ctttcttctt cgcaaacttt tctaaattag cttttgccat ataagtatgg gcaatccctg
    15001 cgatacatgc tgttactgct actatttttg tcatatgaaa ttcctccttt taaaatgata
    15061 gagcttttac caattctttt ttatttctcg aatcttttat tcttttaaca aactcgtcat
    15121 ccatcaatct tccagataat tctgctaaaa tacgtaaatg attagtacct ttatcagtgt
    15181 cttttattgc taacaaaaag acgagatcaa ctggttgatc atctaatgcg ccccattcaa
    15241 cagaccgctt taacttggct acagccactg ttgattcaat tactgctagg cttttgccat
    15301 gaggaatggc gatatttttc ccaattcctg tggtcatatg gttttctctg tcaaatacgt
    15361 ctttttgaaa ttgtttgtgg tcagacacaa acccattagt ctctaacaat ttcgataatt
    15421 tttcgactac acttttttta tcagatgcat ttaaatctat cactatatta tttatattga
    15481 ttttttcact gatcatttat aagccttctt tcaatatata aactaatttt tcaaacgacg
    15541 aactcgtttt taaatttgct aataactcat gattcgaagc gatttcattg aatgtttcat
    15601 aaattttatt caattcaggt tttacttttt cattaagtgc aaaaaagaaa acagaatgaa
    15661 caagtgtccc atcccaatca attccttctg gattaacgta gacacagatt actggctttt
    15721 ttataaaatc tacttcgata tgcggcatag caaaatcatt catcgctgtt gaagatagtt
    15781 tttctctttt gattgctccc tctagtatcc cttctttcgc gtaattttct tttaccaatt
    15841 ttttggtaat tatatttaaa acgttcactt gattatcctt tatttggtgg gtaaaaaagc
    15901 aaaatcgttt atctaataaa tttagaaaag catcttctgt ttgttcattt aactgtagat
    15961 actgttttac ttttctgaca tctgccccta acatcaaagg actaacaaca ataacaggaa
    16021 aatttgtatt ttcaatcgga atcgtgctta taacaagttc atcagtgact tttctatctt
    16081 gtagttcatt gatgcttagt acatcttcta tctcaaccat atctgcaaaa ccgttattta
    16141 ttctttgctc taataatttt gatgttccat atccgctact acaaactaat atcactttcg
    16201 ttttgtcagg tttgtaacga tccaaaagtg attgaatatg gagtgttacg taagcgattt
    16261 catcttcatt aaaatgtata cagaatctct cttcgatttc ctctaataaa tctacactta
    16321 tcataaatga ccttttaaaa gaatatttga ttttgtccgt ataagggtta tagatattta
    16381 ctcccaattt gagtctttta actgctgcat tgagatgtaa tactagatta ttgatcaatt
    16441 cttcatcagg ataaaattca tttaatttaa ttcttactaa ctcttgtagc tcaacaccca
    16501 tatctaaata ggaaagcttc gtttgatcta agtcgtgatt catagcattt ttttcaatcg
    16561 aagtaatgtg aatgtcaaca taagcttgtt catctattgg cacaataatt gaaaatgctt
    16621 tttcaatcat tttgattaaa ataagtgtat tctttgagat ttcttcttca gaattattac
    16681 tacgtgataa gaaattgctt gtttcaatcc tttctatcgc aatagcaagg tggatagcta
    16741 aagtttgaaa ttcataatct gtaaatttca attgtgtctt attcatgtat cgattaagca
    16801 attcggctac ttttttcaat atgtccttat ttaagaaatt attcgcttca ccaatgaaat
    16861 tgatattcaa gtctaagaga tctgttttac tgttagatgc tgtaaagcca ccccaataat
    16921 agttcacaac ttctgacatc aaccttcttt tacttttttc atcagctttg atgcgcacgc
    16981 catttcgatc tgaaactaag gacatcccat gcttgtttag tctttttcta acatctttca
    17041 ttgtgctttc taccgtcgac ctgctcacgt ataattcgtc agcaagattt tgaatcttta
    17101 gagaatcatt agtattcaat aatttagcaa acacataagt tagacggtcc tccggagagt
    17161 ctgcgatatt gattttatga ctggataata aatttcgaag tgtttctttt tttccaataa
    17221 gcgaaacgcc ttttttaggt tgaaccaaca tttgtacatc tggcattttt tggattattt
    17281 ctttcaaaat taaaatatct ttgctaattg ttttcgtaga tacatataat tgttctgata
    17341 attctttgta agttgtgtgc tcattttcaa tcaaaaagta tagtattttc atttgccgtt
    17401 cgctaaattc gatgcccatt atcttcacct cctgaataca gcttaagtgt acaattatta
    17461 tctctatttt catacagaag aaagttcttg attaatgtag aagtttctaa tattcttgat
    17521 tatttca
//
LOCUS       DAZKOK010000058        17461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000058 DAZKOK010000000
VERSION     DAZKOK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.058"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..705)
                     /locus_tag="ACG90W_001651"
     CDS             complement(160..705)
                     /locus_tag="ACG90W_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0478011.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(717..1409)
                     /locus_tag="ACG90W_001652"
     CDS             complement(717..1409)
                     /locus_tag="ACG90W_001652"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0478012.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1428..1712)
                     /gene="macP"
                     /locus_tag="ACG90W_001653"
     CDS             complement(1428..1712)
                     /gene="macP"
                     /locus_tag="ACG90W_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0478013.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1725..2288)
                     /locus_tag="ACG90W_001654"
     CDS             complement(1725..2288)
                     /locus_tag="ACG90W_001654"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0478014.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2516..3178
                     /locus_tag="ACG90W_001655"
     CDS             2516..3178
                     /locus_tag="ACG90W_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0478015.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3302..4498
                     /locus_tag="ACG90W_001656"
     CDS             3302..4498
                     /locus_tag="ACG90W_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0478016.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4599..5975)
                     /locus_tag="ACG90W_001657"
     CDS             complement(4599..5975)
                     /locus_tag="ACG90W_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0478017.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6012..6878)
                     /locus_tag="ACG90W_001658"
     CDS             complement(6012..6878)
                     /locus_tag="ACG90W_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0478018.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6967..7992)
                     /locus_tag="ACG90W_001659"
     CDS             complement(6967..7992)
                     /locus_tag="ACG90W_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0478019.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8010..8684)
                     /locus_tag="ACG90W_001660"
     CDS             complement(8010..8684)
                     /locus_tag="ACG90W_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0478020.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8868..9761)
                     /locus_tag="ACG90W_001661"
     CDS             complement(8868..9761)
                     /locus_tag="ACG90W_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0478021.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9835..11532)
                     /locus_tag="ACG90W_001662"
     CDS             complement(9835..11532)
                     /locus_tag="ACG90W_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0478022.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11525..12838)
                     /locus_tag="ACG90W_001663"
     CDS             complement(11525..12838)
                     /locus_tag="ACG90W_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0478023.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12838..16779)
                     /locus_tag="ACG90W_001664"
     CDS             complement(12838..16779)
                     /locus_tag="ACG90W_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0478024.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16888..17346)
                     /locus_tag="ACG90W_001665"
     CDS             complement(16888..17346)
                     /locus_tag="ACG90W_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0478025.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4910 a         3905 c         3029 g         5617 t
ORIGIN      
        1 aatataactt aagttgttga taataaagag atttattaac ggaaaaagaa taagtttttt
       61 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
      121 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
      181 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacgga
      241 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
      301 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
      361 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
      421 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
      481 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
      541 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
      601 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
      661 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
      721 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
      781 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
      841 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
      901 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
      961 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
     1021 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
     1081 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
     1141 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
     1201 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
     1261 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
     1321 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
     1381 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
     1441 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
     1501 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
     1561 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
     1621 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
     1681 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
     1741 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
     1801 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
     1861 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
     1921 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
     1981 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
     2041 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
     2101 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
     2161 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
     2221 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
     2281 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
     2341 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
     2401 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
     2461 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
     2521 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
     2581 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
     2641 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
     2701 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
     2761 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
     2821 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
     2881 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
     2941 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
     3001 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
     3061 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
     3121 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
     3181 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
     3241 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
     3301 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
     3361 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
     3421 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
     3481 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
     3541 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
     3601 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
     3661 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
     3721 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
     3781 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
     3841 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
     3901 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
     3961 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
     4021 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
     4081 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
     4141 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
     4201 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
     4261 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
     4321 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
     4381 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
     4441 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
     4501 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
     4561 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
     4621 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
     4681 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt
     4741 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
     4801 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
     4861 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
     4921 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
     4981 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
     5041 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
     5101 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
     5161 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
     5221 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
     5281 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
     5341 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
     5401 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
     5461 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
     5521 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
     5581 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
     5641 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
     5701 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
     5761 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
     5821 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
     5881 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
     5941 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
     6001 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
     6061 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
     6121 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
     6181 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
     6241 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
     6301 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
     6361 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
     6421 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
     6481 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
     6541 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
     6601 ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca
     6661 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
     6721 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
     6781 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
     6841 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
     6901 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
     6961 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
     7021 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
     7081 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
     7141 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
     7201 aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc
     7261 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
     7321 atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa
     7381 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat
     7441 gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc
     7501 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
     7561 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
     7621 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
     7681 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc
     7741 actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
     7801 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
     7861 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
     7921 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
     7981 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
     8041 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
     8101 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
     8161 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
     8221 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
     8281 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
     8341 aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct
     8401 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
     8461 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
     8521 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
     8581 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
     8641 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
     8701 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
     8761 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct
     8821 cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt
     8881 ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc
     8941 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
     9001 tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg
     9061 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta
     9121 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
     9181 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
     9241 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
     9301 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
     9361 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc
     9421 atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa
     9481 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
     9541 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
     9601 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
     9661 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
     9721 ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt
     9781 aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac
     9841 ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc
     9901 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
     9961 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
    10021 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc
    10081 ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa
    10141 gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct
    10201 atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc
    10261 agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc
    10321 atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc
    10381 ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc
    10441 atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg
    10501 tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat
    10561 cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat
    10621 cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt
    10681 atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa
    10741 ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata
    10801 agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc
    10861 ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc
    10921 tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat
    10981 catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata
    11041 agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac
    11101 ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg
    11161 atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga
    11221 attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac
    11281 ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt
    11341 agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa
    11401 tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt
    11461 cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat
    11521 ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata
    11581 aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc
    11641 attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt
    11701 cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt
    11761 tcccggattc taattcagcc atctcctgtc cgttaactac tacatattgt ggttggcctt
    11821 ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct
    11881 cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt
    11941 gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca
    12001 aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat
    12061 ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg
    12121 gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta
    12181 gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcggtt gattctgtcg
    12241 ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca
    12301 atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt
    12361 ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc
    12421 cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat
    12481 tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag
    12541 cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg
    12601 gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat
    12661 cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca
    12721 acacgaattc cacgacatct tcccctgatt cttctgccga catcgcatgt ggaagaagga
    12781 ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta
    12841 ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc
    12901 catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa
    12961 cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag
    13021 ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga
    13081 aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc
    13141 aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc
    13201 aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag
    13261 ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact
    13321 attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat
    13381 tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc
    13441 atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc
    13501 aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata
    13561 cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg
    13621 gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat
    13681 cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag
    13741 cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc
    13801 ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa
    13861 tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc
    13921 acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt
    13981 gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc
    14041 tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg
    14101 atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa
    14161 cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg
    14221 atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat
    14281 tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt
    14341 tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg
    14401 tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg
    14461 tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc
    14521 atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatcta ctaactgata
    14581 acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc
    14641 tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt
    14701 ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc
    14761 agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc
    14821 gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc
    14881 ctccaggttc tgaatatagg ccatattgtt cactgtgcaa aaatttccgt cttgagctcc
    14941 cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg
    15001 agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc
    15061 tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc
    15121 gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg
    15181 tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt
    15241 agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg
    15301 cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc
    15361 atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact
    15421 tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc
    15481 agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc
    15541 aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt
    15601 tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc
    15661 attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc
    15721 aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact
    15781 tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt
    15841 ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag
    15901 ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc
    15961 cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt
    16021 attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg
    16081 gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt
    16141 ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac
    16201 gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat agacattgta
    16261 aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc
    16321 tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc
    16381 atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga
    16441 tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact
    16501 atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt
    16561 gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaact tcgggtagcg
    16621 atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc
    16681 tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc
    16741 ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg
    16801 aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct
    16861 ttcatcaacc accacttgaa gaggtagtta cccttcgttc acatcgggga tcttttctgt
    16921 ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc
    16981 ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc
    17041 tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg
    17101 tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc
    17161 ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc
    17221 ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg
    17281 gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc
    17341 tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaac
    17401 aatgcccatc taattataat ttaatttaat tttaatttta aatcaaatga tatccttttt
    17461 a
//
LOCUS       DAZKOK010000059        16789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000059 DAZKOK010000000
VERSION     DAZKOK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.059"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACG90W_001666"
     CDS             complement(<1..384)
                     /locus_tag="ACG90W_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478026.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ACG90W_001667"
     CDS             complement(569..967)
                     /locus_tag="ACG90W_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0478027.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ACG90W_001668"
     CDS             complement(964..1737)
                     /locus_tag="ACG90W_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0478028.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ACG90W_001669"
     CDS             complement(1734..2417)
                     /locus_tag="ACG90W_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0478029.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ACG90W_001670"
     CDS             complement(2584..3471)
                     /locus_tag="ACG90W_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478030.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ACG90W_001671"
     CDS             complement(3468..4337)
                     /locus_tag="ACG90W_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478031.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ACG90W_001672"
     CDS             complement(4474..5760)
                     /locus_tag="ACG90W_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0478032.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="ACG90W_001673"
     CDS             6033..6611
                     /locus_tag="ACG90W_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0478033.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="ACG90W_001674"
     CDS             6611..7540
                     /locus_tag="ACG90W_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478034.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="ACG90W_001675"
     CDS             7537..9273
                     /locus_tag="ACG90W_001675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0478035.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG90W_001676"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG90W_001676"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0478036.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="ACG90W_001677"
     CDS             complement(10169..10678)
                     /locus_tag="ACG90W_001677"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0478037.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG90W_001678"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG90W_001678"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0478038.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="ACG90W_001679"
     CDS             complement(11979..13070)
                     /locus_tag="ACG90W_001679"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0478039.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG90W_001680"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG90W_001680"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0478040.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG90W_001681"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG90W_001681"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0478041.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG90W_001682"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG90W_001682"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0478042.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4854 a         3707 c         2968 g         5260 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaa
//
LOCUS       DAZKOK010000060        16325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000060 DAZKOK010000000
VERSION     DAZKOK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.060"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1548
                     /locus_tag="ACG90W_001683"
     CDS             121..1548
                     /locus_tag="ACG90W_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478043.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1640..2005)
                     /locus_tag="ACG90W_001684"
     CDS             complement(1640..2005)
                     /locus_tag="ACG90W_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0478044.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2002..2367)
                     /locus_tag="ACG90W_001685"
     CDS             complement(2002..2367)
                     /locus_tag="ACG90W_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0478045.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2559..2807)
                     /locus_tag="ACG90W_001686"
     CDS             complement(2559..2807)
                     /locus_tag="ACG90W_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478046.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2806..2934
                     /locus_tag="ACG90W_001687"
     CDS             2806..2934
                     /locus_tag="ACG90W_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0478047.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2991..4685
                     /locus_tag="ACG90W_001688"
     CDS             2991..4685
                     /locus_tag="ACG90W_001688"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0478048.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4752..4958
                     /locus_tag="ACG90W_001689"
     CDS             4752..4958
                     /locus_tag="ACG90W_001689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478049.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5336..6469
                     /locus_tag="ACG90W_001690"
     CDS             5336..6469
                     /locus_tag="ACG90W_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0478050.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6500..6820
                     /locus_tag="ACG90W_001691"
     CDS             6500..6820
                     /locus_tag="ACG90W_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0478051.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6849..7313)
                     /locus_tag="ACG90W_001692"
     CDS             complement(6849..7313)
                     /locus_tag="ACG90W_001692"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478052.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7445..8698)
                     /locus_tag="ACG90W_001693"
     CDS             complement(7445..8698)
                     /locus_tag="ACG90W_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0478053.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8727..9044)
                     /locus_tag="ACG90W_001694"
     CDS             complement(8727..9044)
                     /locus_tag="ACG90W_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0478054.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9070..9390)
                     /locus_tag="ACG90W_001695"
     CDS             complement(9070..9390)
                     /locus_tag="ACG90W_001695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0478055.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9854..11269)
                     /locus_tag="ACG90W_001696"
     CDS             complement(9854..11269)
                     /locus_tag="ACG90W_001696"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0478056.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11286..12602)
                     /locus_tag="ACG90W_001697"
     CDS             complement(11286..12602)
                     /locus_tag="ACG90W_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0478057.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12752..13450)
                     /locus_tag="ACG90W_001698"
     CDS             complement(12752..13450)
                     /locus_tag="ACG90W_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0478058.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13739..14728)
                     /locus_tag="ACG90W_001699"
     CDS             complement(13739..14728)
                     /locus_tag="ACG90W_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0478059.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14808..15641)
                     /gene="msrA"
                     /locus_tag="ACG90W_001700"
     CDS             complement(14808..15641)
                     /gene="msrA"
                     /locus_tag="ACG90W_001700"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0478060.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15816..>16325)
                     /locus_tag="ACG90W_001701"
     CDS             complement(15816..>16325)
                     /locus_tag="ACG90W_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478061.1"
                     /translation="TVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDY
                     FLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYA
                     YLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCL
                     FFKIVFHKR"
BASE COUNT         5089 a         3285 c         2770 g         5181 t
ORIGIN      
        1 aaaaaacctt ttttaacggc atttatatgc tttttcagaa attaactgat gatttcaaaa
       61 agaaaacatg tcataatatt tcccatattg tgcaaaaaaa tacaaggcgg aggctctact
      121 atgattgaag aatatattga gaaaaatatt ttaagacagc tgtttttatg tgggcaattc
      181 tatgtaaata aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc
      241 actttactca atgatatcaa taacataaaa aaagaatttg aagaacagat cgcttataca
      301 catcgggaaa aggaccgtta cactttatat ttttcagaac atatacctcg atgcaagatc
      361 atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa
      421 gatgaacctg attatcttca attaactgaa tgtgaattca tctctgtcag caaagcttat
      481 tcattaaaaa aacaagtatt agcttatttt aatgactgcg ggatcgaaat cgaccgctac
      541 tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta
      601 ggcggtttta attcttggga gctacctgaa tcgttttttg aacgtgccga tcgtttcatt
      661 gaatccgtaa cggaaaatag tggcagattt tatgataaag aaaataaaga aatcttatcg
      721 attggttttg ctatcagttt cctacgccag caagtgtgtg ctgtgacaat agattcaaaa
      781 tttatcgaag aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc atgggaaaac
      841 actgatttcc aaacctatta taaaaaagaa gaattcgcct ttattttgac actattcaat
      901 ctttgtaatt atggtttcca ttcttatcag ttgattgcgg aagactttca acagctacac
      961 caagtgttta ttgacaatac tcccgaaata aaagaactag ttgctacctt tgaatctcac
     1021 ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt
     1081 tcggcttggg ataactatca attattcatg ccagaaaaat tttatctttt aaatgaggaa
     1141 cagacaaatt tatataaaga agtccagaca attttttcca gctggtccag ccagttacct
     1201 tatgatctac ggttgaatcc taattgcatg cgggcttttg tgatcgaatt gagcggaatt
     1261 ctccgtctga caaaagaaca tctgactatc tatatcgtca caaattcgga tgttcattat
     1321 ctgatttatc gggaagcctt ggaagctgta actactttcg attttcaagt tgcaccaaca
     1381 atttattcaa gtatatctga tataaagaag tatgcgcagc agtcctcaaa ccgggtatta
     1441 tgtgaacgta cattatatac accagatgcg gtccaatatg aaaatattat tcctatttcg
     1501 atcaatacga tcgatcgagc tatcatttca gctgtgcaaa acaaataagg ggctgtgaca
     1561 aaaatcatgt cacagcctct tatttgttga agatgcccat ttctgtaaca atctcttgat
     1621 acacgtcagg ctcacaacct cacttatttt ggacatctac gggtccgatc tctttattta
     1681 aaaaataaga aatgatggtt cggataacta ccaacgccgc tagttggaaa atatcctgca
     1741 aggtaggttt gataatcgat tcgatgatcc cagctgaaac aagaatttcc aaacttagaa
     1801 gtatatagcc tcccaacatc tttttaatag cattgttctc gctgactcgg tcgctttgca
     1861 cttttgtatg gacaaataaa tatttcaaaa aatccttgat tgatagacaa acaccccaaa
     1921 ccaataaaca gatagcaaag atatcaagtc ccagtacaag tttttcgaaa tatggaagaa
     1981 gaaactgcat caactctgtc gtcatcctac ctctcctctt ctttgttttt ctttgtcttc
     2041 tatttcctga ttcaagaaat aagaaatgac tgttcgaatg ataataataa gtgcaagctt
     2101 caaaatatct gtccaagtcg gcttgataac tgactcgatg atatcagcaa cgactagtac
     2161 ttccaagctt aacaaaatat aacttgctag cagttttctt gtatctgcat tacgagtatt
     2221 acgttctgca tagtttttct tgacatgaga ccaatcaaaa agtttgaata gaacaagaac
     2281 gatcccgaca attaagatac cgatagaaca aatatttaaa atagcaatga tccattctag
     2341 aaattcaatt aaatttgagc gaaacatata cgctacctac tttcttttaa tttgttccta
     2401 ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt ctaatgataa
     2461 cggttcgctt aaatttttct ttttcactct cccatttttc tccctccatc cataaatcgt
     2521 ggaagtatcc aaaacaacgt agcaagttaa aataaggctt agtccgtcat cagcttttca
     2581 aagaagcact gtctcggtgc tttttttgta cccaaatttc taaaaataga accatacacc
     2641 tcctcaaatc ttttcttctt acctacccta tttttattta caggaaaatc agacacttct
     2701 tcttttttgt caaaatttcc tttttcttct cgtcctatca tttggcattc taataaaaac
     2761 gaactatcct tggatataga aagagaaaac aaggaggcgt ttttcatgag taaaaaatta
     2821 atcaaagtcg gcatcggttt aggtttgctt gcccttggcg ctgcatatct aggaaaaaag
     2881 acaggactat tcgaagatga cagtcattta tatgatgaat tcgaatcaat ctagcatcct
     2941 atttagttga atcactacgg acatacttgg aagacattgg aggaatcaca atgagaaaaa
     3001 gtaaagcaat aatgattggt gccggtattg ccaatatggc agcggctgtt tatttgattc
     3061 aagaaggcaa atggaaaggg gatcagatca ccttttattc attggatgat cacggttcaa
     3121 atgatggtgc accgaccgac actgtagtgg atgaatattg gaataaaaac catccattag
     3181 aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct taattaccgt acctatgtag
     3241 atttgatgga tctgttaagt cgaatacctt ctgttactga gccagggatg actgctgaag
     3301 aagatacgag agattttgat gcaaaacacc gaacctttga caaagcacgt ttgatggaag
     3361 gtggcaaagg aatcattgat ggcggaaaac tgggattcaa taataaagac cgtattcttc
     3421 tgactaaact gattgctatg cctgattcag aagaagaaaa actagacaat atcactatcg
     3481 aagaatattt caaagatgat ccacactttt ttgaaacaaa cttctggttt atgtgggaaa
     3541 caacatttgc tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa
     3601 tgatttatga attcactcaa atcgaacatc tagtgggcgt caatcgaaca cgttacaatc
     3661 aatacgaaag tattatgctt cctttgatcg agtacttgaa agaacaagga tgcaaaatca
     3721 ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac accgatgcaa gatgaaatca
     3781 ctgtgactgg tttgaaaatt accaatacac tgactcaaga agaagaatac gttgtggtcg
     3841 atgaagatac agcagttatt ttcactaatg ggtccattac agattcagct actttaggag
     3901 attacgaaac accagctgta gaaaatatgg attatggtgc agcttctagt ctctggaaaa
     3961 aagccagtga acgtttctac aacttgggaa atcctgacaa attctttgct gatcgagatg
     4021 caagcgaatg ggtcagcttt accctgacga cgaaaaacca tttgttgttg aatgaaatca
     4081 cccgtatcac gactcaagaa ccaggaaatg ccttgaattc atttatttca accacaccga
     4141 tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat caaccgcatt
     4201 ttacgtctca gaaaccaaat gaaacagtta tttggggtta tttcctttat ccaagacgac
     4261 gtggcgaatt tgtggataaa cagtatatta aaatgaatgg aaaagaaatg ctagaagaac
     4321 tgattggaca actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag
     4381 aaattttcga cagcgtaata aataatatcc ctgtctatat gccttatgct tccgcgttgt
     4441 tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc taaacattcg accaatctgg
     4501 cctttacagg agaatttgtc gagcagcctt atcagatgat ctttactgaa caaagtgctg
     4561 tacgttctgg agaaatcgct gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca
     4621 aaacaccgcg ttacgacaaa gatattccta ctctagctcg tgccgcaaaa aaaatgtttg
     4681 aataatcaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca accaggctaa
     4741 aacagcttct tatgtttttg atttggttgg acttattacc gcagatgcaa gcttttgaac
     4801 accgtttacc aagaggttat gaaaaagctg ccctgcatca agtaataaga gcagccaagc
     4861 taaaacagct tcttatgttt ttgacttggt tagacttatt accgcagatg caagcttttg
     4921 aacaccgttt accaagaggt tatgaaaaag gcgtttagtt tcgagtatga agaaaaattt
     4981 tgtgaaaatg gttcctcacg tttttgcata aatttgactt aataccgaga aactgatccc
     5041 ttctgtcata acctcttttt atttatgtct gtttcttttt gaaaacaact attttttcaa
     5101 cgtttactct cttgccaatc caacatcatc taacggatac aactatttca atgattacca
     5161 ttacactaaa aaatcggaaa cacacctctc cgaacacaaa tacataagac tatagaatgt
     5221 ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt aaaatgaaat
     5281 tatctgtagt actaataatg cagacaattt tatgaatctg aaaggagcat ctccaatgaa
     5341 agcagtcact tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg atccaaaaat
     5401 attagaacca acagatgcaa tcatccgtat cacagcaaca gccatttgcg gctccgatct
     5461 gcatctttac catcatggtg aagcagtttt ggaaaaaggg tatgttgtag gacacgaacc
     5521 aatgggaatc gttgaagaag tgggatctca agttcaaaca ttgaaaaaag ggcaacgtgt
     5581 ggttatccct tttaatatta gttgtggtca ttgtcatttc tgcctgaatc acatggaaag
     5641 ccaatgtgat gaatcaaatc cagatcagtt atatggcggg cttttcggat ttggcaagtt
     5701 gaacggtaat cattggggtg gtcaagcgga atatctaaga gttccttatg cagactctac
     5761 ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa gttttatttc tatcagatat
     5821 tttgcccaca gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg atacagttgt
     5881 gattctaggt tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta tggctggagc
     5941 agagcgcgta atcgcagtag atccagtaca gcaccgtcta gataaagcgg ctaattacaa
     6001 tggggttgaa acttatttct tagaagacac ggcacaagct ggaacagaat tgtatgaact
     6061 ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga atggacggat tagagcctgt
     6121 caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc acaatctctc cattacagat
     6181 ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta actggtgttt atatgactcc
     6241 agcttcttct tacccgctgc aagaattttt tatgcgggat atcgctgtca agcatggaca
     6301 ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg attgcagaag gactattcga
     6361 tcctacacaa atcattactc attcgctgcc actatcagaa gcagcacaag catatgagat
     6421 ctttgacaaa aaagaagata aaaatataaa agttgttttg aaaccttgaa ttcagtaaaa
     6481 aatacaggtg attttcttta tgcccaaaat caaacgaaat acccatgaga tcgaaatagg
     6541 aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc aaaatcgtta
     6601 cacattgatc tcactggctg tcgatttatt aaccgggatt ttctatattt ttggaagcct
     6661 tgcttcctta actaaaatcc ccgatcgata tggcacctat ttttatttgg ctggtgctat
     6721 tttcttgacg attcgtccta ttctcagaat cgtaaggaac atttttattt acgacgaaaa
     6781 aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga gaatttttca aatctcatgc
     6841 ctcttttttc accctatcgt atcactttga tacagacgag cggtcgtccc actttcttcc
     6901 ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg atctgtaatt
     6961 aagtaaagtt tttcgtaccc aagttgatgc atttcttttc tgatttccgc taatatccgc
     7021 ttggctatcc cctgcttacg cttttccggt tcaacaaaca gggcacaaag atttggtgtc
     7081 agatcagggc ggttatggaa gtcgttttca atgacgcccg ctcctccgac gatggtttct
     7141 ttttcatcta agaccacata ccattgagga atactttcct tattagcgat tccagtacga
     7201 atactttcct catacacaac agcaggaact ccccattttg atgaaaacca attcgcgctt
     7261 tgttttacta attctgggtg ttctcttaat ttgatccagt tatatgtaac caatgtttaa
     7321 acctccgact aaaataaaac gtttacaaaa attatacaag tctttatgaa aattagcaat
     7381 gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc acttgttctc cgttttttga
     7441 ttaatcaagc atctaaagct tcttttcttc tttcttggat cgtagctacc ttgacgaacg
     7501 gtagatagat caaaatagaa agaaccaaat tgacagcggc tagtacacca cctgcaatac
     7561 tttttgttgc caatacaccg ccaataattg gtggagttac ccatggaggc ataacggtag
     7621 ctgctggcac taaacctgta gaagtcgcaa agtaagcaac actaactaag accattggtg
     7681 tcaaaataaa tggaatgaac atgatcgggt tcaacacgat aggtaaccca aacatcgttg
     7741 gttcattaat attgaagaca cctggcgcaa tcgacaagtt tgttacaacc atatatggtt
     7801 tgtttttacg tcctactaaa taaatagcta acagtaaacc taaagttgca cctgttcctc
     7861 ctaagttgac aaagctgtca aagaaaggtt tgttcacgat aaacggtgcg ctatgtccag
     7921 ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt gattgtttgc atcaaaggat
     7981 cgaccatatt cgctccgtgt aaaccaaaga accataagaa tggtgtgata aatgcaagca
     8041 gtaaggcaga tggataagag tttgcaagtc ccataaatgg ttcttggacc actgtataga
     8101 aagaacctac gatatcatca acaccgaatg cagcaaaaat tgcagcaact aaagcaaacg
     8161 cagagatagt gatcattgct ggtagtaatg aagcaaatgc ttttgcaact gccggtggca
     8221 caccatcagg cattttgatg atcaattttt catttccgct taaacgtaca aagatttctg
     8281 ttgaaataat cccaacaata atagcaataa acaaaccgtt tgagctcata cctaatgctc
     8341 cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc ggaaacaccg tcttttcggt
     8401 agccgttagc taaatgataa gcaagcaaga aagcaatcaa gacagaaaga atggcaaatg
     8461 taccgttcca agcagcagcc ccgaatcctt tccatgattc accaccaaaa attgagttca
     8521 tcaattcttg gaacccggga atcggtaagt tattgatcat tactgctaat gctcctaaga
     8581 tcattaacgg catactcacc ataaatgagt cccgaatcgc aactaaatgc cgttgtgcac
     8641 caatttttga tgctacaggt ataaaatgtt tctccataaa tgcaacaaat ttatccataa
     8701 atcttccctc ttttctttac tgtagattac gcgttcaatc gacgatacag atcaatgatc
     8761 tcttttgcta aatctgtaaa agctatagat gtcatcagat gatcttgact gtggaccgtc
     8821 aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg tcagcatctc tgtttgagag
     8881 tgatgtgctt ggactaaaga ttcctctgca tctttgattt tttcatctgc taaagtgaaa
     8941 tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc tttttgcatt tccgccaaac
     9001 atgatcagac ccatgatggc ttctagattt ttttgatctt ccatttattg tatcctccga
     9061 taatttttat tatttgttta ttagtttttc tgcttggtcg agtacttttt caccattcat
     9121 catgccatag tcagccatat tgattacatc taaaggaatg cctttaggtg ctagcttttc
     9181 agcaaattga ttttgcataa agcgtacttg cggtcctaaa agtaaaacgt ctacattttt
     9241 agattctagt tgattatccg catctgaagc agatacagca aagatgtctg cttccattcc
     9301 ttttgcttct gctgcctttt gcatttttgt gactaggaga cttgtactca ttccagctga
     9361 acatacaagc atgattgttt tcttttccat tttaaacacg ttcctttcta tacttacact
     9421 tgtatataag caagtagcgt gccaacttta aaagcgcttt aaaaagttga tatcatcaca
     9481 tttaaggtct ataccaagtt gcatacacac taaaaaagaa agcgcacact aaacacacaa
     9541 attaattttt attacctttt ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt
     9601 gtcacaactt ttttcaaata aacggtattc aaaagcttgc atctgcggta ataagtccaa
     9661 ccaagttaaa aacataagat gttgttttta ctttgctatt cttattactt gatgcaggac
     9721 agctttttca caacctctca ataaacggtg ttcaatcagc tgaccctggg tattagctca
     9781 aaacgctgga ttcacaacct cttttatttc aaataaccag taaatacaat gaacatctac
     9841 aaaaaacttc tatttattta tagatttcgt caggcaaatc caattctctc ttatcaatca
     9901 aagtctgata ccacgaagca gattttttca atgttctgct tcgttcatcg ttcaattcga
     9961 ttcgaactaa tccatatcga ttcttaaatg cattcattgg tgacacacag tcagtgaatg
    10021 cccataacat atagccttcg caatttgcgc cttcttctgt tgctttgatc aagtaatgca
    10081 aatgctgtcc gataaattcg atacggtaat catcttgcac gattcctttc tcatccatat
    10141 actgttcttc atattctctt cccataccgt tttctgttat cagccaaggg atattatgat
    10201 aattttcttt tagatacatg gctatatcat acatgatttt cggatagatt tcccatcccc
    10261 tggaattatt cattttcttc ccaggtaaat caaagacttc ataatatcgt tcaggatgga
    10321 aaggtgtctc ttcattccat tgataacgag gggcgcgaac gcgtttagga taatattgat
    10381 tgattccaag gaaatctact gtatgatgct gaatcgtttc taaatcctct gttgcaggat
    10441 cgaaataaat cgattgtttt tgacacaact ccaggaactc ttctgtgtag tatcctttta
    10501 ccatcggatc aaaaaatact cgattataaa acaaatcata catataagca gctttttggt
    10561 cttctggaga attggatcga gcatagacca tttcaggatt taaaatacag ccaatccttc
    10621 caccataatt tccgctgcgg taaatctcta ctgctttagc cgttgctaaa actttatgat
    10681 aattccacat catccacttt tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat
    10741 agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa ccagtttttt accctatcac
    10801 cgtaacgatc aaaggcaatt tgagcatact tagcataaaa atcaactact tttcttgaag
    10861 accagccatc atatttttcc aaaagaaaag ctgggacttc ataatgttcc aaacaaatca
    10921 tcggttctac gtctgcttcg atcaaagagt cgatcatttc acttatatgc tgagcatatt
    10981 cttcatctac tatacagttc tcataatcag taaaaaaacg agcccaattg atagaagtcc
    11041 gataatgtgt cagatggatg gatttcatca gatccacatc ctgctggtat tgttccataa
    11101 aattagttgc tacggctggg ccatacccat tatgccaaac aaaatgttca tttttatacc
    11161 agctatctat aaacgaatcc tgtccttctt tttttccttt ccaaccttcc gtttgccaag
    11221 cagaagatgc agcaccaaga ataaaatttt ttggaatagt aattttcatg atttcctcct
    11281 gttttttaga tcccatcatt ttccacacga tttgctactt ttacaaatgg cagatagatc
    11341 aaaatagaaa caataataca aacaagttgc gtaataactg caccgatatt tcctgctgtt
    11401 gccagataag cattgacgat cggcggcatt ggccaaggga ccattacaac tgctttcgcc
    11461 gcaaatccta aagcggttgc aaaataacca atcgttccag tgataagagg cgttaagata
    11521 aacggaatag ctaaaattgg attcagcatg atcggcatac caaaaataac tggttcattg
    11581 atgttgaaga tcccaggagc aagggataac ttggtgattt gtttcatatc ttcccgtttt
    11641 ccaactaaca atactgcaat cagtaaaccg atcgtgactc cagacccgcc catactcata
    11701 tacatatccc aaaatggcat agtaataatg ttcggtactt cttttccagc ttcaaaagct
    11761 tccgtattta cagcaattgc cgctaaaagc aacggttccc gtatcggctt caccatttga
    11821 tttccatgga taccaatcac ccaaaagact tgcgcaataa acatcaatag caaaataccc
    11881 ggtaaccctt gtacgacacc ttctaatggg cgttgtacga cattgtaaat gatatcataa
    11941 aggtacattc ctgtaagttc tttgatgata aatcccgcag ccgcgatacc cgatacagtc
    12001 agaatcgtcg ggaataaagc agaaaaacta gcagaaacat tcgttggcac ggtatcaggc
    12061 attctcagct gtagtttctt ctgtttcctc aaccagcaat aaagttcaat agaaaggatg
    12121 gagaccaaca ttcccaaaaa taaccctttc gtatctgtat attgtttagc caatacgttt
    12181 tctactataa cagtctgatt ttcattcact aattcataaa cagttggatt aacagataaa
    12241 taagacatca ctgcgatgat accagggaat acaccatcca ctttattcag cttggctaat
    12301 tcgatcccta tcagaaaaac agcatagatg gtcaagaaac ttagtgtgac atagctgatg
    12361 gcagatgcga taggctttaa ttcttctaaa aaacgcaaga aacttatctg tgctaagcca
    12421 ttttccgagt caaagaccat tgctgaaaac aatgtgccaa aagcacccgt aataattacc
    12481 ggaatcaatg cagcaaatgc gttttttatc accatgatat accttaaatt attgattctc
    12541 gttgccacgt tccctaaaac attggctaat ttatccataa aactcattcc agatcccctc
    12601 attcctgctt cttctccttg acgtcatctc tttttgtcta ttagtcagcg cttacatttc
    12661 gatgaacaga aactatttac tcctcttttt taccaaatat aaaacaaaaa ttcatctgat
    12721 gtatttatca taaaatacat cagatgaact gtcaattagt ttctttttct cctattattt
    12781 tctgtaattc tctccgattt attgccatat ggacagtcat tgcatcaaac gcagcaagtg
    12841 cattccgcct ttcgatggcc tgaacgatct ctgtatgaat atcgatcgtc tcgattttca
    12901 gatgctggat atcataattt ttattaaaca aagcaattga ttcattaatg attgggataa
    12961 tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta
    13021 attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc aatcttttgc gttaattttt
    13081 tcatttggac gatttgattc ttcgttgcat attccgctgc caaacctgcc atcttaggct
    13141 ccaataaata tctcagttca aataagtctc gtatcatccg atcctgatct tcaatcaaag
    13201 aaaaaccgag tgggtcattg gcaatccctc gcttatcact gacaaatgtc cccgctcctt
    13261 gccgaacttc aaggatattc ctggaagcta gagcccgcac agcttctcgc accgtgcttc
    13321 gccccacctc taaataatcg gacaattcat attcattcgg cagtttatct cctacttgca
    13381 actgcatttc ctttaaatat ctaaggatct tgtcagtagt tacttctact agggttttat
    13441 tttcttccaa accacatacc tccttttcat ctgatgtatt ttttcgtaat tattataaca
    13501 cgtttgaatc atctgatgat tttttgtata caaaattgat taatccgttc atagacatac
    13561 aaacgaagaa gatatttacc ataaaaaaga ctaggataaa attcactcct agccttcgat
    13621 catttttatt ttgaacgaca aaaaatatga tgttcaataa cagacgatct tcattcgatt
    13681 cttgatgtca tcttttgttt tttttaatat atggataaaa agtttgatta catcgtttct
    13741 attctagaat ttggtcgatt ctcaattctt cttctggtga gaaatctaaa ttatccatca
    13801 ttcgaacact ttcttgtaac tgacttaacc gacttgctcc gaccagtaca cttgtaacag
    13861 atttttgtcg caaattccat gcaagggcca tttgcgctaa tgattgtcct cgtgatacgg
    13921 cgattgtttg cagtgctttg attttttcca gagttgatcc aacttgttct tctgaaagga
    13981 atggtatttc cttacgatgt gctctagaat cttcaggaat accatgtaga taacgatttg
    14041 tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc tagcccttct tcttctaata
    14101 ctggagatag atcgtcttct agtagtgcac gagaaaacat attgtagcgc atctgatgaa
    14161 taatgaacgg tgcttccttt ctttttaaaa tttcagtcat tttttttgta tcttctccat
    14221 tatagttgga tataccaata tataatgctt ttccttgtct tacaagttgc atcaaagctt
    14281 ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg atgatagaag atgtcaacat
    14341 aatctagccc cattctttgg agactttggt cacaacttga aatgatggat ttacgagagc
    14401 cccattcccc atatggccct tcccacatat gataacctgc tttgctagaa ataatcagtt
    14461 catcacggta ggcttgaaaa tcttctctaa aaatacgccc aaagtttttc tctgcagctc
    14521 cagcagggcc tccataatta ttcgcaaggt cgaaatgggt aattcccatg tcgaatgcgc
    14581 ctcttaaaat ctctctttga ttatggatag gatcataatc accaaaattt tgccataatc
    14641 cgagagataa aatcggtaat ttcaaacctg aatgaccgga tttacgatat tccatttttt
    14701 cataacgtgc actatctgca atatacaatt tttctcctcc tcatcaaatc tttttaaaac
    14761 gctttctttt ttctattttt agtttactac attttaaaga aaaggagtta atcaaaatga
    14821 ttttttcgta tatttttttg ccatttgttt tttactcgtt gatagatcag aaattgatta
    14881 cgtgcttgct ggattttttt ataacgtttc ggttgttttt tgtaaaattg ctgatgatag
    14941 ttttctgctg gccaaaacgt accggctggt tcgatttttg tgacgattgg ttggtggaaa
    15001 gttcctgaac tgtctagttt ctgttttgat tgttccgcta attctttttg tcgttcgact
    15061 tgataaaaaa tcactggacg atattgcttc cctctatctt gaaattgtcc aaatgtatct
    15121 gtgggatcag taatttgcca atagattgtc agcaactcgc tataacttat ttctcttgtg
    15181 tcataaatga tctccacagc ttccacatga cctgtgtaac caccacttac ttgatcataa
    15241 tttggttttt caacatgtcc gcccgtgtat cctgacaaaa cagataggat cccttttttc
    15301 gattcaaacg gctcgaccat acaccagaaa catccccctg caaatactgc acgttcttga
    15361 tagtcagcat cttgcatggc atgcttggaa aagtcatatt tttgctcctt atcaaatcct
    15421 tccgtaatca ataaataaaa gtccattaca tcaggcgtca gattcattcg gatagctaat
    15481 ggacgtagtg ttgcttctag ttttgataac tgttctttaa cggatatatt ttctcttatg
    15541 tcattctttg tctgaatcaa tacttttcgt tcccaatctc gtgtattcgg attcaaaatc
    15601 agattataaa gtgtttgaat gaatatttgt tcctcatcca tagattctcc tcctttccga
    15661 agaagacaag ctagtgactt tagctgtaat ttatcataaa gtgaggaaat aagcgattac
    15721 ttcatccccc tacagatttt tcgtataaaa aagagagaat ccttatcgac agattctcct
    15781 ttttaatcaa tttattttat aaatgaaaag ttaatttacc ttttatggaa aactatttta
    15841 aaaaataaac ataaatatgc agttaatgta acaagataag aaataatagt cgttactgta
    15901 taacttgtgc ttactttaat atcagcaact cctttgactc cgcttatttg taccaatcca
    15961 ttagcattca cctgctcctt tacaggttga tgatttattt ctacattcgt tcctaaataa
    16021 gcatatacag gaagtgtgat tacttggctc ttctcattag ttactctata tatgatagaa
    16081 ctaccagttg ttttataagg aaggtttacc cattgactgc caatctttgc ttcgtgtttt
    16141 attatatgct ccttattctc caatgaagcg ataggagaat aatcatataa aaaataatcc
    16201 attcctttag tggtatacgc ctctactgtt tctttgttta caacaatatg tcccggctga
    16261 gaaattattt tttgattgaa atttttttta ctagaaacag ttaaagtgat taataacaaa
    16321 acagt
//
LOCUS       DAZKOK010000061        16208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000061 DAZKOK010000000
VERSION     DAZKOK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.061"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG90W_001702"
     CDS             226..894
                     /locus_tag="ACG90W_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0478062.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG90W_001703"
     CDS             complement(1094..1414)
                     /locus_tag="ACG90W_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478063.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG90W_001704"
     CDS             complement(1451..2833)
                     /locus_tag="ACG90W_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0478064.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG90W_001705"
     CDS             complement(2989..4176)
                     /locus_tag="ACG90W_001705"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0478065.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG90W_001706"
     CDS             complement(4183..5214)
                     /locus_tag="ACG90W_001706"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0478066.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG90W_001707"
     CDS             complement(5230..6684)
                     /locus_tag="ACG90W_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0478067.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG90W_001708"
     CDS             6859..7764
                     /locus_tag="ACG90W_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0478068.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG90W_001709"
     CDS             7855..8532
                     /locus_tag="ACG90W_001709"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0478069.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG90W_001710"
     CDS             8529..8699
                     /locus_tag="ACG90W_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478070.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG90W_001711"
     CDS             8813..9709
                     /locus_tag="ACG90W_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0478071.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG90W_001712"
     CDS             complement(9764..11134)
                     /locus_tag="ACG90W_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0478072.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG90W_001713"
     CDS             11372..12118
                     /locus_tag="ACG90W_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478073.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG90W_001714"
     CDS             12115..12954
                     /locus_tag="ACG90W_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0478074.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG90W_001715"
     CDS             12955..13905
                     /locus_tag="ACG90W_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0478075.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG90W_001716"
     CDS             14046..14798
                     /locus_tag="ACG90W_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478076.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG90W_001717"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG90W_001717"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0478077.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5262 a         3018 c         3250 g         4678 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa catagaaaaa cgattacttt gcttatacaa caaggctttt atggttttgt
    16201 atcaaaaa
//
LOCUS       DAZKOK010000062        15746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000062 DAZKOK010000000
VERSION     DAZKOK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.062"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..726)
                     /locus_tag="ACG90W_001718"
     CDS             complement(28..726)
                     /locus_tag="ACG90W_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0478078.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="ACG90W_001719"
     CDS             complement(719..2071)
                     /locus_tag="ACG90W_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0478079.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="ACG90W_001720"
     CDS             complement(2294..3694)
                     /locus_tag="ACG90W_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0478080.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="ACG90W_001721"
     CDS             3855..4592
                     /locus_tag="ACG90W_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0478081.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="ACG90W_001722"
     CDS             4717..5196
                     /locus_tag="ACG90W_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0478082.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="ACG90W_001723"
     CDS             5193..5960
                     /locus_tag="ACG90W_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0478083.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="ACG90W_001724"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="ACG90W_001724"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0478084.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="ACG90W_001725"
     CDS             complement(6863..7465)
                     /locus_tag="ACG90W_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0478085.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..7801
                     /locus_tag="ACG90W_001726"
     CDS             7571..7801
                     /locus_tag="ACG90W_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478086.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7792..8040
                     /locus_tag="ACG90W_001727"
     CDS             7792..8040
                     /locus_tag="ACG90W_001727"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478087.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8146..8730
                     /locus_tag="ACG90W_001728"
     CDS             8146..8730
                     /locus_tag="ACG90W_001728"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0478088.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="ACG90W_001729"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="ACG90W_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0478089.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="ACG90W_001730"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="ACG90W_001730"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0478090.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="ACG90W_001731"
     CDS             10631..10810
                     /locus_tag="ACG90W_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478091.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="ACG90W_001732"
     CDS             10832..11857
                     /locus_tag="ACG90W_001732"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0478092.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /gene="glyA"
                     /locus_tag="ACG90W_001733"
     CDS             12032..13276
                     /gene="glyA"
                     /locus_tag="ACG90W_001733"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0478093.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="ACG90W_001734"
     CDS             13429..14376
                     /locus_tag="ACG90W_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0478094.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="ACG90W_001735"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="ACG90W_001735"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0478095.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="ACG90W_001736"
     CDS             complement(15278..15520)
                     /locus_tag="ACG90W_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478096.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="ACG90W_001737"
     tRNA            15641..15713
                     /locus_tag="ACG90W_001737"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3196 g         4378 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg attttt
//
LOCUS       DAZKOK010000063        15738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000063 DAZKOK010000000
VERSION     DAZKOK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.063"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG90W_001738"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG90W_001738"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0478097.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG90W_001739"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG90W_001739"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0478098.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG90W_001740"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG90W_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0478099.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG90W_001741"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG90W_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0478100.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG90W_001742"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG90W_001742"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0478101.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG90W_001743"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG90W_001743"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0478102.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACG90W_001744"
     CDS             complement(8557..9027)
                     /locus_tag="ACG90W_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0478103.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACG90W_001745"
     CDS             complement(9073..9498)
                     /locus_tag="ACG90W_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0478104.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACG90W_001746"
     CDS             complement(9565..9834)
                     /locus_tag="ACG90W_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0478105.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACG90W_001747"
     CDS             complement(9845..11440)
                     /locus_tag="ACG90W_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0478106.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG90W_001748"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG90W_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0478107.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG90W_001749"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG90W_001749"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0478108.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         4078 a         3523 c         2768 g         5369 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaa
//
LOCUS       DAZKOK010000064        15673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000064 DAZKOK010000000
VERSION     DAZKOK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.064"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            235..1875
                     /locus_tag="ACG90W_001750"
     CDS             235..1875
                     /locus_tag="ACG90W_001750"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0478109.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2029..3351
                     /locus_tag="ACG90W_001751"
     CDS             2029..3351
                     /locus_tag="ACG90W_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0478110.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3413..3844)
                     /locus_tag="ACG90W_001752"
     CDS             complement(3413..3844)
                     /locus_tag="ACG90W_001752"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478111.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3908..4744)
                     /locus_tag="ACG90W_001753"
     CDS             complement(3908..4744)
                     /locus_tag="ACG90W_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0478112.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4934..5236)
                     /locus_tag="ACG90W_001754"
     CDS             complement(4934..5236)
                     /locus_tag="ACG90W_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0478113.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="ACG90W_001755"
     CDS             complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="ACG90W_001755"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0478114.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5690..5956)
                     /locus_tag="ACG90W_001756"
     CDS             complement(5690..5956)
                     /locus_tag="ACG90W_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0478115.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6210..6935)
                     /locus_tag="ACG90W_001757"
     CDS             complement(6210..6935)
                     /locus_tag="ACG90W_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0478116.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7301..7371)
                     /locus_tag="ACG90W_001758"
     tRNA            complement(7301..7371)
                     /locus_tag="ACG90W_001758"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7337..7339),aa:Gly,seq:ccc)
     gene            7488..8240
                     /locus_tag="ACG90W_001759"
     CDS             7488..8240
                     /locus_tag="ACG90W_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0478117.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8256..8735
                     /locus_tag="ACG90W_001760"
     CDS             8256..8735
                     /locus_tag="ACG90W_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0478118.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8986..10566)
                     /locus_tag="ACG90W_001761"
     CDS             complement(8986..10566)
                     /locus_tag="ACG90W_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0478119.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10559..11302)
                     /locus_tag="ACG90W_001762"
     CDS             complement(10559..11302)
                     /locus_tag="ACG90W_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478120.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11478..12254)
                     /locus_tag="ACG90W_001763"
     CDS             complement(11478..12254)
                     /locus_tag="ACG90W_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0478121.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12502..13338)
                     /locus_tag="ACG90W_001764"
     CDS             complement(12502..13338)
                     /locus_tag="ACG90W_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0478122.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13349..14278)
                     /locus_tag="ACG90W_001765"
     CDS             complement(13349..14278)
                     /locus_tag="ACG90W_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0478123.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="ACG90W_001766"
     CDS             14470..14823
                     /locus_tag="ACG90W_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0478124.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="ACG90W_001767"
     CDS             complement(14897..15553)
                     /locus_tag="ACG90W_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0478125.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
BASE COUNT         4803 a         3120 c         2717 g         5033 t
ORIGIN      
        1 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
       61 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
      121 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
      181 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
      241 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      301 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
      361 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
      421 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
      481 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      541 aacgaaattt tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat
      601 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
      661 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
      721 ggtaaattga tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta
      781 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
      841 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
      901 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
      961 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
     1021 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
     1081 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
     1141 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1201 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
     1261 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
     1321 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
     1381 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
     1441 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
     1501 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
     1561 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
     1621 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1681 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1741 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
     1801 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
     1861 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1921 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
     1981 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     2041 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
     2101 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
     2161 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
     2221 ttatttcatt ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc
     2281 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
     2341 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
     2401 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2461 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
     2521 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
     2581 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
     2641 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
     2701 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
     2761 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
     2821 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
     2881 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
     2941 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
     3001 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
     3061 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
     3121 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
     3181 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
     3241 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
     3301 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3361 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
     3421 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
     3481 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
     3541 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
     3601 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
     3661 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
     3721 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
     3781 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
     3841 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
     3901 ccttttttca cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt
     3961 ctaatgtttg caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa
     4021 tcgcttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4081 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4141 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     4201 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     4261 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     4321 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     4381 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     4441 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     4501 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     4561 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     4621 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     4681 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     4741 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     4801 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
     4861 gtgctaaacg aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat
     4921 cgattctttt gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat
     4981 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
     5041 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa
     5101 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg
     5161 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat
     5221 ggttatgttc tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct
     5281 gagccgtcta aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt
     5341 cgtttcgctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
     5401 agtcgttcat cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat
     5461 gacatggaag cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact
     5521 acaaaacggt ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct
     5581 tcttctgcct cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg
     5641 ctcacagcaa cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca
     5701 ctccatgatt tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac
     5761 ggcgaatcaa atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca
     5821 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
     5881 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
     5941 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
     6001 acacccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat
     6061 aaattaagaa acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct
     6121 ctttccataa agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata
     6181 aagttcataa aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc
     6241 tcataattga tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
     6301 ttgtcttcaa tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg
     6361 aaatgaggag tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata
     6421 tcattttcgt gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt
     6481 ttaatgttat tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac
     6541 cattgtccgt cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct
     6601 ctcttttcag taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc
     6661 aatttgtcta tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt
     6721 gtaaactccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
     6781 acatccgcta cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag
     6841 atatctgaat ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg
     6901 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc
     6961 cctaaatctt aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa
     7021 aaaataaaac cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct
     7081 ctactcaatc acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta
     7141 atactcaaaa ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca
     7201 tctgcggtaa taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct
     7261 tattacttga tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa
     7321 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa
     7381 acagtatacg cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg
     7441 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     7501 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     7561 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     7621 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     7681 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     7741 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     7801 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     7861 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     7921 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     7981 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     8041 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     8101 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     8161 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     8221 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     8281 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     8341 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     8401 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     8461 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     8521 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     8581 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     8641 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     8701 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     8761 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     8821 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     8881 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     8941 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     9001 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     9061 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     9121 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     9181 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     9241 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     9301 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     9361 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     9421 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     9481 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     9541 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     9601 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     9661 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     9721 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     9781 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     9841 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     9901 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     9961 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
    10021 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
    10081 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
    10141 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
    10201 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
    10261 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
    10321 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
    10381 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
    10441 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
    10501 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
    10561 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
    10621 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
    10681 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
    10741 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
    10801 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
    10861 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
    10921 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
    10981 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
    11041 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
    11101 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
    11161 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
    11221 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
    11281 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
    11341 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
    11401 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
    11461 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
    11521 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
    11581 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
    11641 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
    11701 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
    11761 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
    11821 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
    11881 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
    11941 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
    12001 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
    12061 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
    12121 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
    12181 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
    12241 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
    12301 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
    12361 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
    12421 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
    12481 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
    12541 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
    12601 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
    12661 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
    12721 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
    12781 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
    12841 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
    12901 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
    12961 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
    13021 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
    13081 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
    13141 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
    13201 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
    13261 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
    13321 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
    13381 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
    13441 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
    13501 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
    13561 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
    13621 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
    13681 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
    13741 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
    13801 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
    13861 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
    13921 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
    13981 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
    14041 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
    14101 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
    14161 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
    14221 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
    14281 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt att
//
LOCUS       DAZKOK010000065        15384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000065 DAZKOK010000000
VERSION     DAZKOK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.065"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1018..1359)
                     /locus_tag="ACG90W_001768"
     CDS             complement(1018..1359)
                     /locus_tag="ACG90W_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0478126.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1359..1589)
                     /locus_tag="ACG90W_001769"
     CDS             complement(1359..1589)
                     /locus_tag="ACG90W_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0478127.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1876..2214
                     /locus_tag="ACG90W_001770"
     CDS             1876..2214
                     /locus_tag="ACG90W_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478128.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2419..2981)
                     /locus_tag="ACG90W_001771"
                     /pseudo
     CDS             complement(2419..2981)
                     /locus_tag="ACG90W_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3713..4294)
                     /locus_tag="ACG90W_001772"
     CDS             complement(3713..4294)
                     /locus_tag="ACG90W_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0478129.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4477..5103)
                     /locus_tag="ACG90W_001773"
     CDS             complement(4477..5103)
                     /locus_tag="ACG90W_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0478130.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5125..6456)
                     /locus_tag="ACG90W_001774"
     CDS             complement(5125..6456)
                     /locus_tag="ACG90W_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0478131.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6611..7012)
                     /locus_tag="ACG90W_001775"
     CDS             complement(6611..7012)
                     /locus_tag="ACG90W_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0478132.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7098..7304)
                     /locus_tag="ACG90W_001776"
     CDS             complement(7098..7304)
                     /locus_tag="ACG90W_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478133.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7399..8901)
                     /locus_tag="ACG90W_001777"
     CDS             complement(7399..8901)
                     /locus_tag="ACG90W_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0478134.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8919..9374)
                     /gene="argR"
                     /locus_tag="ACG90W_001778"
     CDS             complement(8919..9374)
                     /gene="argR"
                     /locus_tag="ACG90W_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0478135.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9472..10089)
                     /locus_tag="ACG90W_001779"
     CDS             complement(9472..10089)
                     /locus_tag="ACG90W_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0478136.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10310..11683
                     /locus_tag="ACG90W_001780"
     CDS             10310..11683
                     /locus_tag="ACG90W_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0478137.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11758..12870
                     /locus_tag="ACG90W_001781"
     CDS             11758..12870
                     /locus_tag="ACG90W_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0478138.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12942..14579)
                     /locus_tag="ACG90W_001782"
     CDS             complement(12942..14579)
                     /locus_tag="ACG90W_001782"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0478139.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14747..15355)
                     /locus_tag="ACG90W_001783"
     CDS             complement(14747..15355)
                     /locus_tag="ACG90W_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0478140.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4256 a         3159 c         2686 g         5283 t
ORIGIN      
        1 ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa
       61 tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca acatataccc
      121 tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt
      181 ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc
      241 aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
      301 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
      361 caattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
      421 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
      481 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
      541 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
      601 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
      661 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
      721 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
      781 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
      841 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
      901 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
      961 ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta
     1021 aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat
     1081 ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc
     1141 ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt
     1201 aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag
     1261 tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat
     1321 atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca
     1381 ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat
     1441 ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat
     1501 tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt
     1561 ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat
     1621 ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg ttttgtttaa
     1681 agtatatctt attggatata aatagaaata aattgtagcc agttcatttt ttctttaact
     1741 aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa atactatagc
     1801 taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca
     1861 acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt ttgctctgac
     1921 aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac tgttcggatc
     1981 aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag acaagacttt
     2041 gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt acatgatcca
     2101 actcatatca atgtggataa atggcaacct cttatctata aattcagaga atatacaact
     2161 gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc ataatgataa
     2221 acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa tagaagctat
     2281 acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat gagcatttct
     2341 tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg ccgctctttc
     2401 tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct gctgagtaat
     2461 gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt cagtcaagac
     2521 aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag tcattatgat
     2581 tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat acactatttt
     2641 ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcattgg gaaaacacaa
     2701 cgattttgca aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca
     2761 ttaatcatca attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg
     2821 aggatgtttt cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca
     2881 gctgctaatt ctgctgcctg cttcggttgt ttcgtctggg cttctcgata ataattctga
     2941 aggagctctg actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt
     3001 tttttctatc atacactgca acaagaagtt cgcaccaaca aaacgctttc aatttttgta
     3061 gaactaaaaa agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact
     3121 cctcggtcac aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct
     3181 tgtgactcgg agtgaaacgg caggcttacg acctctgggt aaacggtgtt caaaagccag
     3241 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gctattttca caaaattttt
     3301 cttaatactc gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc
     3361 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agcagctatt tttatttggc
     3421 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctccactaaa cggtgttcaa
     3481 tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     3541 tgtttctcta atactcaggt caaagcgctg atttcacaac ctctaataca cggtgttcca
     3601 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt
     3661 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa
     3721 ccttttgcga agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata
     3781 gatcctgtgt gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg
     3841 cttgacggtc tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt
     3901 tcgtatactt gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca
     3961 ctgtactcaa ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct
     4021 catgatcagc gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag
     4081 catttaagat gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg
     4141 ctagacaaac ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag
     4201 aagctgctag aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt
     4261 tggtcgcttc caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat
     4321 agtcctatat tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt
     4381 gccaaattat ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt
     4441 gattcaagaa ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc
     4501 ctctgttgtc atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc
     4561 ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac
     4621 caattcacgc atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt
     4681 gtcgattcca ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt
     4741 atgaggagag ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc
     4801 ggctcctgaa ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc
     4861 aagcatcata tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata
     4921 atgaggagaa ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat
     4981 attttcgatc gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc
     5041 tggtttcaag aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa
     5101 catggtggat tctccattcg ttatttattt taatacatca tttagagctt cactcattgt
     5161 cggatgagaa tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag
     5221 cgaaatcaag ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa
     5281 gtcagtttca gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc
     5341 tttagggaca ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc
     5401 ttgtttttct gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac
     5461 agctttccgg ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg
     5521 ataatcatct agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca
     5581 aatgttgtct gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag
     5641 tttaacatct gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc
     5701 caaaactttt gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc
     5761 tgttgattct tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc
     5821 atttgtcatg tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc
     5881 agaatgggca tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc
     5941 caagccaata tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat
     6001 cgcttgcgta gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat
     6061 tggttcagaa ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt
     6121 attcgtcact tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc
     6181 agttgcctca tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc
     6241 tttccgagca acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt
     6301 agaaggaata cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac
     6361 gactaaaacc gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc
     6421 aaaaccgatc acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct
     6481 accgattggt acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta
     6541 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
     6601 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
     6661 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
     6721 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
     6781 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
     6841 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
     6901 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
     6961 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
     7021 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
     7081 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
     7141 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
     7201 ttttctacat aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc
     7261 ccataaacca atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc
     7321 tggtctccct accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag
     7381 cctttcaaaa agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa
     7441 taattttgct aattttacta acgccaactt gttctttccg aattcatcat tttctatctg
     7501 taatactagt aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg
     7561 ctcttctaga tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc
     7621 ttcttccaac agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc
     7681 gctgaacaat tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg
     7741 ttcatctaat gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat
     7801 gacatatgca ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc
     7861 gatggctagc tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt
     7921 tcgatagccg gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag
     7981 atgagaagaa gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat
     8041 tgtacttaat actgcgatca aattatttcg attgatcact gttccagaag cactgacaac
     8101 tgcagaacga tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac
     8161 agcctgcttg atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa
     8221 tcggccatct ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac
     8281 tttttcagca aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt
     8341 tagtggtttg ctttctagtg ttccaatccc ctttttaatt tttgtgacta aatcttgcgc
     8401 ataaggtgtc aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg
     8461 ctgctgtgat aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa
     8521 ttttattcca ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca
     8581 cgagaactgt tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc
     8641 gatgacatgt atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc
     8701 gcttatccta ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt
     8761 cttctttttc tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt
     8821 caagtttact tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg
     8881 cagcgctttc atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc
     8941 ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca
     9001 ccggccatcg ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca
     9061 tcggcaccat ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa
     9121 acctcgcgtc taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta
     9181 aataatgtat agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga
     9241 atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc
     9301 agctcttcct gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc
     9361 tgtttctttt tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt
     9421 tcttcatatt ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt
     9481 ttctatttta acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct
     9541 gatgtaaatc tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc
     9601 ttctaatttc tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc
     9661 acaaagacct tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt
     9721 gctagaacgc tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat
     9781 catcaattga taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg
     9841 cagtccccat tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca
     9901 atttttttta ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg
     9961 tttaaagtct tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg
    10021 aacctctagt atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat
    10081 tttttccatt tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt
    10141 tgagtgactt ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt
    10201 aaataaatcg tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta
    10261 taatagaaat cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca
    10321 aaaacagctg cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt
    10381 attgatcgag acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac
    10441 gactgcacaa gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc
    10501 gctgtctact tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct
    10561 gcttttcttg gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt
    10621 tggtcgattg ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat
    10681 cttgacattt gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac
    10741 ggccattgct ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg
    10801 gaatcatatc ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt
    10861 tgctattccc gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg
    10921 aatttgtctt ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag
    10981 ctggattgct ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa
    11041 agtggagcat ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt
    11101 gattggcttc ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt
    11161 ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc
    11221 agcggttggt gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa
    11281 gaagccaatt tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact
    11341 cataggacac gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat
    11401 cggattatct tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca
    11461 aggagacggg aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc
    11521 attagtagcg gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc
    11581 tactacatta ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt
    11641 tgctcgattc gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg
    11701 tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg
    11761 gaaaaaattg ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat
    11821 acatcaaaat tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc
    11881 gctgggatta tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct
    11941 aaagacggcg ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt
    12001 tacgcacaat cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt
    12061 cttgccgaga aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct
    12121 gaacagacat ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt
    12181 ggtggtcgtc tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa
    12241 caaatggagc agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc
    12301 aagggaccgt ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt
    12361 aagcctttat tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc
    12421 ttagcatttc agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga
    12481 gaagctgatt tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac
    12541 gatcaaggct gggatttggc tcctactagc gaagcttata agggattgac taagagtaca
    12601 gaacagttct tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt
    12661 acccgtgctt atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc
    12721 ttactggctg ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat
    12781 ctattagcca acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc
    12841 tccacctata ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga
    12901 gcgatattgc gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc
    12961 aaattctcca aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca
    13021 atgaatcgta aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca
    13081 aaagaaacag gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac
    13141 ttgaattctg ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg
    13201 atttttttat catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa
    13261 aacaactctc gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca
    13321 aatggttcaa tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat
    13381 cgttttgttg ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata
    13441 attggcggaa gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga
    13501 tgatcgactc ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt
    13561 atatctcctg ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga
    13621 caagtaagga aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag
    13681 taagaaggtt ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca
    13741 tgcaagatcg aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca
    13801 gaaccggaca acccaaatac agccatcgta taggtctggt cattactagc atattgtttc
    13861 atcccaccgt ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa
    13921 gtcgcttttt ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga
    13981 gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag
    14041 atataaagat ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa
    14101 ttcgctggct gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc
    14161 agcaaatgac tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa
    14221 aactggcgct tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt
    14281 tccttgtcta agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca
    14341 tcattgaata ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt
    14401 gttaaatctg tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta
    14461 tcaaaaactt catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg
    14521 gataacagtg gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg
    14581 gctcctcctc attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt
    14641 atttgtactg ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa
    14701 cttgaataaa caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt
    14761 ttgctaacca gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt
    14821 ctgctacacg ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt
    14881 gataacggcc gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg
    14941 cgatccattc ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag
    15001 ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg
    15061 tagatgctgt attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg
    15121 ggattgttaa ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag
    15181 caattgcatc gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat
    15241 cacctgattt tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg
    15301 cagtagcacc agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa
    15361 actccttttt ttctccgttt ttta
//
LOCUS       DAZKOK010000066        15314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000066 DAZKOK010000000
VERSION     DAZKOK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.066"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG90W_001784"
     CDS             278..787
                     /locus_tag="ACG90W_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0478141.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG90W_001785"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG90W_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0478142.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG90W_001786"
     CDS             2322..3302
                     /locus_tag="ACG90W_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0478143.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG90W_001787"
     CDS             3424..4842
                     /locus_tag="ACG90W_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0478144.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG90W_001788"
     CDS             4913..6382
                     /locus_tag="ACG90W_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0478145.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG90W_001789"
     CDS             6392..6859
                     /locus_tag="ACG90W_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0478146.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG90W_001790"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG90W_001790"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0478147.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACG90W_001791"
     CDS             7849..10383
                     /locus_tag="ACG90W_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0478148.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACG90W_001792"
     CDS             10539..11312
                     /locus_tag="ACG90W_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0478149.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACG90W_001793"
     CDS             11305..12096
                     /locus_tag="ACG90W_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0478150.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACG90W_001794"
     CDS             12106..12597
                     /locus_tag="ACG90W_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0478151.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACG90W_001795"
     CDS             12615..13025
                     /locus_tag="ACG90W_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0478152.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACG90W_001796"
     CDS             13029..14429
                     /locus_tag="ACG90W_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0478153.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACG90W_001797"
     CDS             14452..14826
                     /locus_tag="ACG90W_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0478154.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5315 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta gagtaagcgc
    15241 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
    15301 tctggaggtt ttct
//
LOCUS       DAZKOK010000067        15258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000067 DAZKOK010000000
VERSION     DAZKOK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.067"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1024
                     /locus_tag="ACG90W_001798"
     CDS             <1..1024
                     /locus_tag="ACG90W_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0478155.1"
                     /translation="KISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLI
                     TGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFF
                     FTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGM
                     IISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRS
                     VFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNI
                     LLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLT
                     VRRLKR"
     gene            1021..1788
                     /locus_tag="ACG90W_001799"
     CDS             1021..1788
                     /locus_tag="ACG90W_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0478156.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1888..3456
                     /gene="liaX"
                     /locus_tag="ACG90W_001800"
     CDS             1888..3456
                     /gene="liaX"
                     /locus_tag="ACG90W_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0478157.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3753..4073
                     /locus_tag="ACG90W_001801"
     CDS             3753..4073
                     /locus_tag="ACG90W_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0478158.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4073..4423
                     /locus_tag="ACG90W_001802"
     CDS             4073..4423
                     /locus_tag="ACG90W_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0478159.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4590..5528
                     /gene="hprK"
                     /locus_tag="ACG90W_001803"
     CDS             4590..5528
                     /gene="hprK"
                     /locus_tag="ACG90W_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0478160.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5543..6373
                     /gene="lgt"
                     /locus_tag="ACG90W_001804"
     CDS             5543..6373
                     /gene="lgt"
                     /locus_tag="ACG90W_001804"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0478161.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6417..7442
                     /locus_tag="ACG90W_001805"
     CDS             6417..7442
                     /locus_tag="ACG90W_001805"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0478162.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7457..8395
                     /gene="galU"
                     /locus_tag="ACG90W_001806"
     CDS             7457..8395
                     /gene="galU"
                     /locus_tag="ACG90W_001806"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0478163.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8520..8960
                     /locus_tag="ACG90W_001807"
     CDS             8520..8960
                     /locus_tag="ACG90W_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0478164.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8963..9565
                     /locus_tag="ACG90W_001808"
     CDS             8963..9565
                     /locus_tag="ACG90W_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0478165.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9628..10731)
                     /locus_tag="ACG90W_001809"
     CDS             complement(9628..10731)
                     /locus_tag="ACG90W_001809"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0478166.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10941..11942
                     /gene="ccpA"
                     /locus_tag="ACG90W_001810"
     CDS             10941..11942
                     /gene="ccpA"
                     /locus_tag="ACG90W_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0478167.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12297..12692
                     /locus_tag="ACG90W_001811"
     CDS             12297..12692
                     /locus_tag="ACG90W_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478168.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12692..12943
                     /locus_tag="ACG90W_001812"
     CDS             12692..12943
                     /locus_tag="ACG90W_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478169.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12986..13381
                     /locus_tag="ACG90W_001813"
     CDS             12986..13381
                     /locus_tag="ACG90W_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478170.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13378..14634
                     /locus_tag="ACG90W_001814"
     CDS             13378..14634
                     /locus_tag="ACG90W_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0478171.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14646..14969
                     /locus_tag="ACG90W_001815"
     CDS             14646..14969
                     /locus_tag="ACG90W_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478172.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14962..15228
                     /locus_tag="ACG90W_001816"
     CDS             14962..15228
                     /locus_tag="ACG90W_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478173.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5245 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aaaaattagc atcatcacta tattttttat ttgtgtgctt acttcatggg ttattcagca
       61 tcaagccgct ttatatgaag tacctgtcat cgagatagaa aaagtacgaa taaaagagga
      121 tcatcaattg attacaggaa aactgatgaa ctcggatcaa aaaggagaat cgatttattt
      181 atcggttcct tatcaaaaaa atcaaataga aacagcaaat ataaaagcga gacaaatgta
      241 tctagtccaa atgcatggag atgaatggca gctagtagca aaaaaaaatg atggctggct
      301 tttcttcttc acaagtatct tcattggcgc catgttgctt attggcggaa aacagggtct
      361 atttgcttta ggaagtctca tcatcaatat cgttttactt ttacttttgt tgctcatctt
      421 tactaaaaca gaagggattt ctctattaaa taaatctatc ctgttcgtcg ttatagggat
      481 gattattagc atattggctt tagatggatg gaatagaagc agtataatca aaatttcagc
      541 tgcagttacg agtgtttttt tagcattttt tatttgtctt ttgacaatga atcattggaa
      601 agaccaagga ctgcgctatg aggaactgaa ttatctgaca cgaccgtatc gttctgtttt
      661 tctttctagc gtactgattg gaacagctgg aggaacactc gataccgtta tcacagtcat
      721 tgcgacatta gaagaattga ctaagcgaca accagaaata acagcgaaac agttatggct
      781 ttcaggaagg caagtgggtc gagatgtgat tggctcgatg accaatatcc ttttgttcgt
      841 ctatatcagt gggtcagttc catcgcttct tttatattta ggaaatcaat ggtctttcaa
      901 agaaacgata gaacagcatt tatcattgga atttttacgc gtcattgctg gcagcttagg
      961 aatagtatta tcgatcccca tctcggtcct cttttttctg actgtaagga ggctgaaacg
     1021 atgaatgtac ttattatact tgttgggatt ttagcaatca gcgtgctact tattggcggt
     1081 acgcaaggta tgtatattct tttaggctta ttcatcaatt tagggatctt tttcctgttg
     1141 ttattcggtt atcatcagaa gtggccgata ctggcattgt cggtcatagg atttttattg
     1201 attgctgtgg ttatcttatt ttttatcaac ggttataatc taaaaatgcg tgcagctttt
     1261 gtgagtatac tgatcttttt attttgtttc ctactattgc ttcctatcac tgatttttta
     1321 gcgatccaag gttttacatc ggtagaacta gaagaattat ctggcttaga taagacttta
     1381 gcgattgatt ttagattact tacgagatcg ttgcttctga ttagtcttag tggtgctgtt
     1441 ttagatgcga gtgtagcaat ttctagtggg acattcgaag tatatcaggc aaatccgcaa
     1501 ttatcttttt accaattgcg ccattctagt ttcgctattg ctaaaaaaat attggcgtca
     1561 acagtaatga cacttctttt tgcattcatg ggaagttctt tagcgttgat cctctggttt
     1621 attgatttag acattccttt tcagcaagtg atcaatgaaa aatcatttgt tctagaatat
     1681 acgatggcta ttcttaccac attgtccgca ctaattgttc ttcctttaac ctgcgtgact
     1741 acagcatatc tctttacaaa aaagaaagaa caactcaaga tggagtaaaa gttacaactt
     1801 aagtcctatg acatttttac tgaatcagtg cataatatgg atgtaaggaa aagcttgcaa
     1861 acaattttgc ttaaggaggc aatttccatg aaagaaagag aacgtatttt agatttagtg
     1921 aaaaaaggaa tcctttcgac tgaagaaggc ttagatttat tagaaagcat ggcaactgaa
     1981 aaagacgaaa agcaaatcaa aaaagaagca gacaaggtaa ctgctagtca taaagaaaaa
     2041 gataaggcaa gtcaattgat cgataaactt gaaaacggcg aagaagaaat ttctgagcca
     2101 gttgatccaa aggaaagaga aagacaagat caagaaaact tagaaaagat cttagatgaa
     2161 ctagcaactg aagcgaataa aacttcggcg cgtctagatg aagtaaatgc tcaattcgct
     2221 gacaataagg cagcacgcaa tgagaaacag gaagcattga tgcagctgaa tacaaaagaa
     2281 gaattaggcg agttgacaga agaagaatta gcacagcgtc aggcgttaga ggcagaaatc
     2341 aaagagttag aagctagtgg cgatacactg ttagaagaac aattgaaatt agaagcagaa
     2401 ttaaaagata tccgtaagaa tcaatggtct gagaaaaaag aaacgtttac tgataagttt
     2461 gaattgccag atgattggaa agatcaagca acagatacac tgaatcaagt aggcgaaaaa
     2521 atgtcagaag ctggatcaca gttaggcaaa ttcttgaaga aaacattcca aactgtttca
     2581 gaaacagtga atgacaacat ggaatggaaa gatgtcagct tacgtgtccc tgggattgcg
     2641 acaacgaaat ttgagcatga attttactat gaagctcctg cagcaagtat ccttgatatc
     2701 aaggcagcaa acggaaacgt gacgttaaaa acgtgggaca gcaacgatgt gaaagttgaa
     2761 gctaagatca aactttacgg aaaaatgggt gctgaaccat ttgaagcatt ctctgaacgg
     2821 agccagattg aggttaatga agatcatatc tccttccaaa ttccaaataa acgtgtgcgt
     2881 gcagaccttg ttttctactt acctaaacga gtatacgacc acgcagctat taaacttcta
     2941 aatggcaaca tcatgattga aacattggaa gctaaagata tttatacaaa atctacgaat
     3001 ggtaacatca tcgtggacca attaactgca acgatgctgg aagtagaagg tgtaaacggc
     3061 aatatcgata ttcgaaacgg gaacatctta gattctatta tcgaaacagt aaatggtaca
     3121 gtgacatttg gtgcgacagc agaaaatctt tctgtgtctt tagtaaatgg tgatgtacgt
     3181 cttacgatta aagaagataa tttgaaaaaa gtagaagcaa gttcagtgaa cggaaatgtc
     3241 aaagtcgctt taccagatgg catcggtttg gaaggacatg ctaaaacaag tcttggaagt
     3301 atcaatagcc gtctgtccaa ttacgaagta gttcgtgaga aaaaagaacg gacaaaccaa
     3361 atgttgcaat tccgtcgtgt ttcagatgat gagatcgcgc aagtgcaact ttcaacgaca
     3421 acaggaagta tctatttaaa agatacagat aaataaagag gttgtgaaag agatgttcag
     3481 ccttgagcaa taagaagcga ttttgtaaaa tagcttcccc tattttacaa aattgtgcct
     3541 tattgccgat agtctatctc ttgaacaccg tttaccaaga gattatgaac ctgccaatta
     3601 tcgccgagtc acaaggaata atttcagaaa aagctcgtca cgttttatga atatggtaat
     3661 ttgtgaccga gaattttgca gaaagacacc gattaagaag aaggttgtca caacgatttt
     3721 cttcatttaa aatagtagaa cagaggtgag acatgatgaa aaaaaagttg acaaaatcta
     3781 ataaaaatgt tgttttgaca gggactttag caggaatagg agaatacatc gggattgatc
     3841 cgactgttat ccgtgtgctt tatgtattcc tgagcttagt tgcttttgga agtccaatta
     3901 ttttatacat tttattagct ttgattatcc catctgcgcg ttctgcacga acaaactatg
     3961 gacataacaa tgcatattat gaaaaaaaca attatcgtga acaaactcgt gaacaaacaa
     4021 aaaaacgcaa agaagcggaa aaaatcgatg atgacgaatg gagtgacttt taatgtcgta
     4081 ctttcaacgt attttagtta atacgttaac attcatatca ttggctgttc tttttcctgc
     4141 gatggtccat gtgagcagta tttggacggc cgttcttgcc agcttcgtat tgtctgtgtt
     4201 gaatatgttt gtcaaaccga ttttgacgat actgtccctg ccctttacat tagtcacatt
     4261 gggattgttt acgtttgtta tcaatgcagc gattcttaaa atgacctcct tttttgtggg
     4321 agcagaacat tttagttttt cttcattcgg tgcagcgttg ttagtagcta tcttgatgtc
     4381 gcttgtcaat gtaattgtca gcgaacataa cagtagaaaa taaaagaaag ctgtgccgaa
     4441 ctatcggcgc agttttttta tttagaatca ggaatgcgtt ttctattaca ttggtaatta
     4501 aataaaaacg ttttttttaa ttggtagtac ttttggctag acaatgataa aatgatagag
     4561 aacactctaa tagaaatgag gtagcatgaa tgtcagaagt tgtaaaagtt agccaattag
     4621 tgaaaaaatt atctttggaa atcgtgacag gcgacgagaa aagcttagac cgtgtcatcg
     4681 taactgggga catctcacgc ccaggactag agttgactgg ttattttaat tattattccc
     4741 ataatcgttt acagctattt ggcagtaaag aaatcagttt tgcggaacgg atgatcccag
     4801 aagagcgttt gatcatcatg agaagattat gtagtgaaga tatgcccgca tttattattt
     4861 caagaggatt accagctcca gaagaaatga tccaggcagc aaatgaaaat gggatagctg
     4921 tactgcgttc tcccatctca acttctcgtt tattaggaga attatccagt tatttagatg
     4981 gtcgccttgc ccctcgaaca agtgtccacg gtgttttagt ggatgtttac ggattaggtg
     5041 tactgattca aggtgacagt gggataggaa aaagtgaaac agcgttggag ttgatcaaac
     5101 gcggccatcg attgatcgca gacgatcgag tagatgttta tcagcaagat gaattaacgg
     5161 tgatcggaga accgcctaaa atcctggaac atctgattga aatccgagga atcgggatca
     5221 ttgatgtaat gaatcttttt ggtgcgagtg cggtacgtgg ctctatgcaa gtccagttgg
     5281 ctgtttatct ggaagcatgg gcaaaagata agaaatacga ccgtctgggc tctgaagaca
     5341 cgaccgttga aatcgcggaa gtgggcattc cacaaataaa aatccctgtc aaaacaggac
     5401 gaaacgttgc aatcatcatt gaagtagccg cgatgaattt ccgtgcacgt acaatgggct
     5461 ttgacgcaac gaaaacattt gaagaaagat taagtcgctt gattgaagaa aactcaggca
     5521 acgactagca aggagtgaca atatgttagc acaaatcaat cgagttgctt ttgagcttgt
     5581 agggattcct atctactggt atgcattgat catcgtatca ggtatcatta tcgctatgtg
     5641 gcttagcagc agagaggctg ttcgggtagg attaaaagca gatgatgtga cagattttat
     5701 gttgtgggga ttgcctatct ccattattgg cgctcgctta tattatgttt tatttgatct
     5761 tccccagtat atagctgatc caatccaaat attcaatatc cgttcaggag gcttagctat
     5821 ttatggcgga ctgattgcag gcgcgatcac gctgtatttt tttactaaat atcattttat
     5881 ttctttgtgg acttttttag atatcgctgc accaagtgta ttattagcac aggcaatcgg
     5941 tcgttgggga aatttcatga accacgaagc atatggacca gacacgacgc gaagcttctt
     6001 agaaagtctg catttaccgc aattcatcat tgataatatg tatatcgaag ggatctatcg
     6061 tcaaccaaca tttctttatg aatcactttg gagtttgatt ggatttattt tgcttttgtt
     6121 tttacgaaag aaaccacacc ttttgaaaaa aggcgaagtc cttttatgtt atctaatgtg
     6181 gtatagtttc gggcgtttct ttattgaagg attacgtaca gacagtcttt actttttagg
     6241 tgtgatccgt atttctcaat tattatctgc tgtgttattt gtcggtacat tgattctatt
     6301 tatttggcgc agaaaaaaag gaaatttacc ggattatgac cgctcattag gaaaaaatca
     6361 acaaattatc tgatttttag agcgttaaaa atgaaaaaaa tttcaggagt ggtaaaatga
     6421 attatagagt agcggtttta ggaccaggct cttggggtac tgctttggca caagtcctcg
     6481 ccgaaaacgg acatgacgtt cggatttggg gacatcgtgc ttcacaagta gaagaaatca
     6541 acacccaaca cacgaatcaa cgttatttac cagatcatta tctacccgat tccatccaag
     6601 cattcaccaa tatggaagat gcagtaaaag acgcagatgc ggtgctattc gtcattccaa
     6661 cgaaagcaat acgttctgtt gcaaaagaac tagtgcctaa attgaataca aagccagtga
     6721 tcatccatgc aagtaaagga ttagaacagg gaacacataa aaggatctct gacatcttgt
     6781 tagaggaaat cccagcagaa aaaagaaaag ctgttgttgt tctttctgga ccaagccatg
     6841 cagaagaagt agcaaaacat gacatcacta cgatcacagc tgcaagtaca gatgcaaaag
     6901 cagcaaaata tgtacaagag ctgttcatga ataattattt tcggatctat accaatcctg
     6961 atgtgatcgg tgtagagatg ggggcagctt tgaaaaatat tattgctatt ggtgctggcg
     7021 ctattcacgg cttagggttt ggcgataatg caaaggcagc aatcatgacg cgcggattag
     7081 ctgagatcag ccgtttaggt gtagcaatgg gtgccaaccc gctgaccttt atcggattaa
     7141 gcggaatagg cgatctagtc gttacttgca caagtgttca ttctaggaac tggcgcgcag
     7201 gcaacttgtt aggacaaggg cagcccctag aagaggtgct agccaatatg ggaatggtcg
     7261 ttgaaggtgt gaatactacc caagcagcga tggaactagc ccaaatgatg gaaatcgaaa
     7321 tgcccatcac tcaaacaatc tatgaagtgc tgtatgaaca aaaagaaatc aaaacagccg
     7381 caaaagaaat catgttacgt gatggaaaaa aagaaaatga attctcatta acaatttttt
     7441 gaaggagcga atgtatatga aagttaaaaa agcagttatt ccagcagcag gattaggaac
     7501 aagattttta ccagcaacaa aagctatggc aaaggaaatg ttgccaattg ttgataaacc
     7561 aacaatccag ttcatcgttg aagaagcgtt gaaatcaggt atcgaagaca tcttgatcgt
     7621 tacaggaaaa gctaaacgac caatcgaaga tcactttgat tcaaatattg aattagaaac
     7681 aaacctaagt gaaaaaggaa agaccgaatt attgaaatta gtagaagaaa cgacagatgt
     7741 caacttgcat ttcatccgtc aatctcatcc aaagggttta ggacatgccg ttcttcaagc
     7801 aagagccttt attggaaatg aaccatttgt cgttatgcta ggcgatgata taatggaaga
     7861 cgaagtacca ttgacacagc aattgatcga tgactatgag cgtacacacg cttctacgat
     7921 tgccgttatg caagttcctc acgatgaaac ttcaaaatac ggaatcatca acccagggga
     7981 agtactggaa gatgggttat acaatgtcaa aaactttgtt gaaaaaccag atccaagcaa
     8041 agcaccaagt gatttagcta ttattgggcg ttacttattg actccagaaa tctttgatgt
     8101 attatcagaa caacaaccag gtgctggaaa tgagatccaa ctaactgacg caatcgatac
     8161 gttgaataaa acacaacgcg tatttgcccg taaattcaca ggtaaacgtt atgacgttgg
     8221 tgacaaattt ggttttatga aaacaagtat tgaatatggt ctgacacatc cagaagtagg
     8281 ggcacctttg cgtgaataca tcatgtcttt aggtaaaaca ttagaagcag aaggaagcaa
     8341 gatcaatcat aatccaaaag gaataacaga aatcaaagaa gaaaagaaaa aataatcttt
     8401 ttttctaacg agcttcagct gctaattgaa taaaaagtag ttgaagctcg tttttatttg
     8461 tttaaaaaac acgaaaatgc tatccttata gaaaagagag acaagaaggg agtttgatca
     8521 tgtcaggagg agaaattgca ggattgatcg cagcaatcgc ctttgcgata cttgtcgttt
     8581 tcatcgttcg cgtattgttg caagtttcca aaactttaga aaaagtagac cagactgtcg
     8641 cagaagcaaa tacgacaatt gagatcgtca cacaagacgt agatctttta tctagacaag
     8701 tagaaggctt gctagtcaaa agtaatgagc tgctcttgga tatcaatgga aaagtcgcaa
     8761 cgatcgatcc attgttcaca gcagttgctg atttaagtga aagtgtatcg gaacttaatc
     8821 attctagtaa aaacatcgtg acgaaagtcg gcggaatcgg aaaaacgaca gctaaagcga
     8881 ctgttgccgg aaaagtcggt ggaacagcga tgaaattttt taaaaacaaa aaacatactg
     8941 aaacgacagg aggaaaataa gaatggctaa aaaaggtgga tttttattag gtgcagtagt
     9001 tggaggtaca gccgcagcaa tcgccgcatt attgttagca cccaaatctg gtaaagaact
     9061 tcgggatgaa ttggctgctc aagcagatga tttgatggac atggcttcag attacacaga
     9121 tatggcaaaa gaaaaatcat ctgagttaac aggtattgca aaagaaaaag cgcaaggtct
     9181 ttctcaacaa gcaggggatc tagcaggcaa tgttaagcag aaggtaagtg aatcagcaga
     9241 ggaagcagga agtgaatcag atgatatctt gttaaacctc aaagaacaat cagctgattt
     9301 atctaatcgt ttcaaaaaaa cagcagaaga tctgaaaaag ccagcagacg aattaggtag
     9361 tattgctaaa gatacttcag aagatatcat gatcgatgta aaagatgctg cagataaagc
     9421 aggagagacg gtcaatgaag gaatgtcaga agcaaaacca gttgcagatg acacaaaaga
     9481 aatgttgaaa atggcaaaag aagatgcaaa agaagctaaa aaagaaatga aagatacaat
     9541 gacagattcg actaccaatc actaagatta ctcatttcta gaataaaaaa ggtgagagcg
     9601 gaccagctct cacctttttt attttcatta ctctaaaaca agtaattctt ttggtgtagt
     9661 cgtgaatggc acacaaccat cacttgtcac atacacacaa tcttcgatac gtacccctac
     9721 tttttcagga atatagattc ctggttcaag agagaagcac atcccttctt caatcactaa
     9781 gtcgtttcct tctacaagag aagggaattc gtggacggtt gttccgattc cgtgacctaa
     9841 acggtgattg aaatattcgc cgtagccata gctgttgatc acgtttcgtg cgatttgatc
     9901 aagttcacct gctgtcacac ctgggcgaac tgcctcttgt gcggcaagct gtgcttcaag
     9961 agtgatgtta tagatttttt cttggaactc agttggtttt tgataagcaa ctgtacgagt
    10021 agcatcactg caatagccat tccaaataac gccaagatcg aataacacta gttcatttgg
    10081 tgaaaccttc gtgcttcctg gtgtaccatg agggcttgca gcatttgctc ctgcaaggac
    10141 taatgtgtcg aaagacattt gtgaaacacc ttgttttttt aattggtatt cgatttctgc
    10201 gataattgct tgttcagtta cgccttcttt tactgttttg aagccaattt caaaggcaac
    10261 atcggcccat ttaccagctt cgatcagttt gtctttttct tctaccgttt tgatcagttg
    10321 cagacgctgg ataatagaag ttagatcggt gtaagaatct gtacctgtca agatatcaga
    10381 gatagcatcg aagcgatcga cagtcagagc attcttttca atacctacat taccaggttg
    10441 cccatagcgt gcgcgaagct cttttccgat gatctcccaa ggattttcgc tgtctaagta
    10501 gccacgtaca tcgtacgccc agctgctgtt ttgagcatct tcaacttcca gagcaggagt
    10561 gaaaaggaaa ggatcttgtt ccagagggat aaaaagagct agtacacgct cgtgaggatc
    10621 actttcatat ccggaaaagt aagcgatgtg gccagggtcg ctgatataag ccaattctac
    10681 ttggtgttgt gccatccatt gacgtagttc attgattttt acgttattca tttggcaacc
    10741 tactttctaa attaattttg tattcaataa ttgtaccact gattgattaa aagaccaacg
    10801 ttcttaaaac tgaaaaaagt aaaaattttc atgaaaacag agataaaacg gttgcaattg
    10861 ttgagattat ttgatatcct attttagaaa tgtaagacgt attttcagaa aacatataaa
    10921 atatgaatag gagaacacaa atggaaaagc aaacaatcac tatctatgat gtagcgagag
    10981 aggcaaatgt ttcgatggct actgtttcac gtgtcgtaaa tggcaatcca aacgtcaaac
    11041 cagcaacaag aaaaaaagtc ttggaagtta tcgatcgctt ggattatcgt ccaaatgcag
    11101 tcgcacgtgg cttggcaagt aagaaaacaa caacagttgg ggtaatcatt cctgatgtca
    11161 gcaatatgtt ctttgcctct ttagcacgtg gtatcgacga tgtggcaaca atgtacaaat
    11221 acaatatcat cttagcaaac tcagatggca acgatcaaaa agaagtcaat gtattgaata
    11281 atttattggc taagcaagtg gacggcgtga tctttatggg tcaccatatc acagacgaaa
    11341 tccgtggaga attttcacgt tcaaaaacgc ctgttgtact agctggttcc attgatccag
    11401 atgaacaagt aggtagcgta aacattgatt atgtgtcagc aacaaaagat gcagtaaata
    11461 aattagcaaa aagcggcaat aaaaaaatcg cttttgtcag cggtgcgctg attgatccta
    11521 tcaatggtca aaaccgtcta aaaggctata aagaagcatt aagcgaaaac aatctaccat
    11581 atagcgaagg attgattttt gaggcacaat acaactttaa agatggtctg tctctagttg
    11641 accggatcca taatagcggt gcaacagctg tctatgtttc agacgatgaa ttagccattg
    11701 gtatcttgga tggtttatta gaccgcggcg taaaagtacc agaagaattc gaaatcatca
    11761 caagcaacaa ttctttactg acagaagtag ctcgtcctcg tttgacaagt atcacacaac
    11821 cattatacga tttaggagcg gtatccatgc gcctattgac gaaaatgatg aataaagaag
    11881 aagtcgaaga aaaaacgatt atcttgcctt atggaatcga agaaaaagga tcaactaaat
    11941 aataaaagat caatcattta aaggttgttc caaaagtggt caacttctgg aacaacgttt
    12001 atacgagaaa gggggttatg acaaaatttt gtcataaccc cttttttttt gtgactttaa
    12061 aaaaagagaa aatggcggaa acaaacctta cgtccatcgt atacgacaca atctgtgcgt
    12121 agatgctaca ttttgttcac tactatcaaa attactttaa aatgaaaacg tgataaaaaa
    12181 gaaagtagaa aagtagataa gtaactctaa aaaatccaaa aaaatccagg ctcgattgcg
    12241 tacttatctt aaaggagaaa caatccatca tatataaccc gaaaggagac ttctatttgc
    12301 ctaagaaaag acgaaagacc atagtcatca ttttgaccat tcttactata ggaatcggag
    12361 ggaaatttta catggacaaa agagaaatgc aaagacagaa agatttactc gcagtagaaa
    12421 aacagtccgt caaagttctg aaaaatacct ttgcggatat caaggaagtg aagattgaaa
    12481 aatctgctcg aaatgaaatg acgggttcat accggatagt tattctaatg actaataaac
    12541 aagatcaatc gatttatttt tcttatagtt tttggaaaga aagaaatgag ataggaagtt
    12601 acggaatagt tgatgaaaaa aagcaaaaag aaggaaatac gctcaataaa gttaaagtaa
    12661 cttactctaa tggaaatgag gaaagtattt aatgattact gacaaacaat atagtgaatt
    12721 aagtgatgct gtttactggc tagatcctaa acatagagac tatgtacctg aaatgcaaga
    12781 aaatttaagt tttaaaatta acggcactat gtataaaatc ctaaaaatca aaaactcctt
    12841 cgacggcatg caggcgatgg ctgtagcgcc aatcgtccat agcaaattgg agaaaaattt
    12901 caaaaataaa aaaattcctg caaactttcg cgtacttaaa taatgaaggt gtcaatactt
    12961 acataaaaag aaaggaggca cgttcttgtc aaggaaaata agaaaactca gcttagccat
    13021 tttattaatc gtcatattag ggatcggagg gaaagtctat atggacaaaa gagaagcaca
    13081 aaggcagaaa gatttactcg cagtagaaaa acagtcagtc aaagttctga aaaatacctt
    13141 tgcggatatc agggaagtga aggtagaaga atttaaaaaa aaccctgtta ccggaagtta
    13201 tggtgcactt gtgacaatga caaataatga agggaaatct gtttattttg attatagttt
    13261 ttggaaggag agaaatgaac ttggtggttt tggcattgag aacagagcgg tacaaaagac
    13321 aggagtcact ataaaaagag taaaagtaat tttttctaac ggacaggagg agttagtatg
    13381 acaataaacg ataagcatta caatgatatt agtgagaagg tttattggct agatcccaaa
    13441 tatccccgct acaatgaggg atacaaaaag aactctgtta aagaatttgc tggtatggaa
    13501 tttcaaatcc tacaaatcaa agactcattg gacggcatgc aggcgatgtc tgtagcgcca
    13561 atcaagaacg gtaaagtgga tctttctcaa gtagtgattg cttttgcagg aacgaactta
    13621 agtgattgga aagatttgga aacggatgca agaagtatca tgaatctcta cgggaataga
    13681 ccaggagcaa gtttttctga aaaagcaaca acaaacaaga ttttatccgt tagtggattg
    13741 aaaacagcgg ggcaaatcaa ttcagcgatt gcatttgcta atgaagtaaa aaaggaatat
    13801 ggagaagctg ctatttctac taccggccat tcattaggtg gattcttagc attatatgta
    13861 gcagcagaga aacaatggaa aaatgtcggc ttcaacgggc cggacccgta tgatctctta
    13921 tcaccagaag caaaaaaatg ggtggaagaa aatcccggta tgctcaccaa ttaccgaaat
    13981 cacgccgatt ttatcggtaa ttttggtggt aatggcacag gtgctgaagt tcggatcagt
    14041 atggaaacag cattcttgaa tctgcttgct tcccacagtt taacctcttg gaagtttgat
    14101 gaaaatggaa agctgattat tcctgaaaat gaaaacaatg ttaaagctcg aaaacaacag
    14161 aaagaaaacc aagcgaccca gttgttttcc tatcagttgc aagaactgaa caacatacgt
    14221 aaaaaactac gattaagtgg cggccggctg acagttaatg aacaaattta cttagatgac
    14281 acacaagcta ggttagtagt ggaaaagtta tcggcaaaaa tgaaaagcgc tatggaagaa
    14341 gttatccgca tcaatcaaag aggaattttt gagctagaga aaaaatggca agaaggcttg
    14401 cggcaagcac gtgttgtagc cccttcgttg actgaacagg aaatcataga agcattagct
    14461 agtgtgggcg ccacaaaaga aaacttagtc gattctgcga aagaagtcta tctgctacga
    14521 atcaataagg caagacgtat gggggaggcc tttgatacat tggcgaagga gatccaagac
    14581 aaaatcaatg aaatcgtggc acgagatcga gaattagcac agcaattcaa ttaaaggaga
    14641 agtagatgaa actagaagaa ttggaaagag attatcgaag tgaacgaaga caattagaag
    14701 ctcaagaaga tgaattgatc cactttaaga atcaaggatt acgattgatg gagcaagagg
    14761 agcagcgact gcatcatgta ttaaagaact tagctttaga taatgagcct ctgtttcaag
    14821 caagacgaag ttattcgaat tatcaggagc attatcaaca agccctacaa gtagaacgac
    14881 gaaaactcta tcagcagcaa gatgaactag aacgttccta tcgtcaaaaa atcagacaaa
    14941 taactatgga gaaatcatct tatgaatgaa tttttaatgc aattactaaa agaattagag
    15001 aaaaatctac atcgatcaaa tgtacgttca gttggtatgg ggatatctca gcaaatttgg
    15061 gcagaaaaac gattgacaga tcaaaactac caaatgactg cagaagaagc tgctcaagaa
    15121 ttttctaacc gacatgtaca cttgaaagga tcagtaaaag ggcaatttgc agaaaaacta
    15181 actgaattat tccaaagaca agaaaagaaa ttaaaacagt ttcgttagta aagttatata
    15241 gaaaaatttt gtgaaaat
//
LOCUS       DAZKOK010000068        15124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000068 DAZKOK010000000
VERSION     DAZKOK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.068"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG90W_001817"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG90W_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0478174.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG90W_001818"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG90W_001818"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0478175.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="ACG90W_001819"
     CDS             1947..2645
                     /locus_tag="ACG90W_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0478176.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="ACG90W_001820"
     CDS             complement(2813..5452)
                     /locus_tag="ACG90W_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0478177.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="ACG90W_001821"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="ACG90W_001821"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0478178.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8469
                     /locus_tag="ACG90W_001822"
                     /pseudo
     CDS             7295..8469
                     /locus_tag="ACG90W_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8473..9114
                     /locus_tag="ACG90W_001823"
     CDS             8473..9114
                     /locus_tag="ACG90W_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478179.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9111..10028
                     /locus_tag="ACG90W_001824"
                     /pseudo
     CDS             9111..10028
                     /locus_tag="ACG90W_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            10025..10696
                     /locus_tag="ACG90W_001825"
     CDS             10025..10696
                     /locus_tag="ACG90W_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478180.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11237..11725)
                     /locus_tag="ACG90W_001826"
     CDS             complement(11237..11725)
                     /locus_tag="ACG90W_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0478181.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11926..12192)
                     /locus_tag="ACG90W_001827"
     CDS             complement(11926..12192)
                     /locus_tag="ACG90W_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0478182.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12192..12455)
                     /locus_tag="ACG90W_001828"
     CDS             complement(12192..12455)
                     /locus_tag="ACG90W_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0478183.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12976..13116
                     /locus_tag="ACG90W_001829"
                     /pseudo
     CDS             12976..13116
                     /locus_tag="ACG90W_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13258..15081)
                     /locus_tag="ACG90W_001830"
     CDS             complement(13258..15081)
                     /locus_tag="ACG90W_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0478184.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4381 a         3243 c         2851 g         4649 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatca atttgataca ccagaaaata ttttacgtca tcctgcaaac
     7981 gatttcgtag aagagttgat tggagaagat cgcctactac aagccaagcc aaatgcgaca
     8041 agagtcggcg aagttatgct aaatacagca atcacgatta caccggaaaa atcattacaa
     8101 gaagcaatca aactgatgag ggaaaaacgt gtagatacgt tgttagttac cgataattcg
     8161 aatgtattaa aaggatttat cgatgtagaa acgttgaata aaaaacgagg aaaagtatcc
     8221 agtgtcggcg acattttgaa taaacatgtt ttttacgtaa aagaatccgc ctatcttcgt
     8281 gataccttgc aacgcatttt aaaacgcggg ctgaaatacg tgccggtcgt tgatgaacag
     8341 aacaaagtcg taggtatcct gactcgtgca tcattagtgg atatcgtcta cgatgtgatt
     8401 tggggagaag atcccgcaac tactgatgtt gcagagtcag ctactcctga agaatctaag
     8461 gaggtctgac ctatgcagca gttttttcat cagcacggtt cagaaattct ctcaaaaggt
     8521 tttgaacatc tttatatttc aggacttgct ttgatcattg gtatcatcat tgctgtaccg
     8581 cttggtgtct tactgacacg ttttccaaaa gctgccacga ttgtgattgg cttaaccagt
     8641 gccttgcaga caatcccttc tcttgcttta ttggcattga tgatcccgct atttggcgta
     8701 ggtaaattac ctgcgattat cgcactcttt atttattcat tattgccgat tttacgtaat
     8761 acgtacattg ggatgaaaaa tgtcgattgg aattatcggg atgtggcaaa aggcatgggg
     8821 atgactgagt tccaatccat tttttccgtt gaattgccca ttgctatgcc aacgatcatg
     8881 gctggtattc gtttagctgc cgtttacgtg attgcatggg ctacacttgc ctcttatatc
     8941 ggcgctggtg gtttaggcga tttgattttt agtggactga ataattacca accagatctc
     9001 attttggcag gtacgatccc tgtaacaatt ttagcactat tggctgattg gcttctaggt
     9061 ctcttggaac accggctgac accgatcgca cttagagagg agaatgaaga atgaaacgta
     9121 tactaaaaat gaccatggtt cttttctctc ttcttatact tgcgagttgt tccttccctg
     9181 gtcttgccag caatacagat gaagacacca tttcgatcac aggtggaatc acttcagaag
     9241 cacagatctt agcaagtcta gttgccggaa tggtttaaca ttatacagat aaaaataccg
     9301 caatcatcaa caatctggct actacaacta tcaaccatca agcgatgatg aacggagatg
     9361 cttcaatttc agcggctcgc tacacaggaa cagatttaac gactacattg aaccttcctc
     9421 ctgaaaaaga tccgaaaaaa gcgtttgcta ccgtgaaaga tgaatttgag aaaagatacg
     9481 gtcaaacgtg gttcccgtcc tatagttttg aaaatacgta tgtctttctt gttcgaaaag
     9541 acaccgcaca aaaataccat ctatctaaag tcagcgatct gaaaaatgta gcggatgaat
     9601 tagtagctgg tgtagatact tcttggatca atcgtaaagg agacggttat gacggcttcc
     9661 aagaaactta tggcttctcc ttcaattcga tattacctat gcagattgga cttgtttatg
     9721 acgcagtcga agcaggaaaa atggatattg ttcttggcta ctctacggat ggacggatcg
     9781 ccagttatga tctagtcatg ttggaagatg atcgtcaatt cttcccacca tatgatgctg
     9841 ctccagttgt agacaacaag atattagctg atacgcctca tttaaaagaa gccttaaata
     9901 agttaggtca taccatcagt acggaaaaaa tgcaggaatt gaactacgaa gcagataata
     9961 atctagtgga accatcagta gtagcacaac gtttcttaca agaaaaccac tattttgaag
    10021 ggaagtgaag caattggata aaatgaatgt ctttcaacaa tttttctatt atttccagga
    10081 aaatggctcc tatatctttg cacaatttat ccgtcacttc ctgatttcta tctatggtgt
    10141 cctctttgca gcagttgtcg ggattccggt tgggatcatg atttctagga gacgtaaatt
    10201 agcaaactgg gtgattcgtt tggcgaatat catccagacg atcccttctc tagccatgat
    10261 ctccatttta atcattggat taggcctggg agtcaatgtc gtgattgtta ctgtattttt
    10321 gtattccctc ttaccgatca tcaagaatac gtataccggg atgatccaag tagacaaaaa
    10381 catccttgat gtcggcaaag gcatggggat gactgctaga caacgactat tcatggtaga
    10441 gttaccactc tctgtttctg ttatcatggc aggcataaga aacgcgttag ttgttgccat
    10501 cgggataacc gcgatcggtg cattcgtcgg tgctggtggt cttggagata tcattatccg
    10561 aggaaccaat gcaacagagg gcacatcgat tattttagca ggtgcattgc caactgcatt
    10621 aatggcgatc atcacggact ggcttttagg tattttggaa agacggctag atccagccag
    10681 ccgtacatca cgctgatgtt attgtaaaaa acaaaagctt ctgcgaccat ttgttggtcg
    10741 caaaggcttt tttagttctg tcatttttaa ggcaataatc cactctgatt cttccctcct
    10801 tatgggcaaa gagttgcttt tcttagctat ttatccattc cgtatactcc aacttcatac
    10861 tgccaacggg ttcatatctg catccaccga ctaagttttt ttcttatccg ctaaggcatt
    10921 ttgccaatcc attttcccat cactcgtcat ggaattcata ccacatgatc tctaaatact
    10981 cgtctgcttg ttctttgaat tgggcatcaa ttcttacatc ctttggcgtg gctttcattt
    11041 aagccttttc catcagtcct cgattgttcg atgccttcag ctgcatcatc aaaactggtg
    11101 actgccgtat cggtaacttc gatcatcttt tatacaatcg ggaacttgcc atcctcatcc
    11161 ttggctaaaa acatatctag ccattccagc ttctcgccat gggaggtttg cggttcctaa
    11221 aaccggtaca cgattcttat ggactgcatc cgtcgcaact agacttggcg atacgatgat
    11281 ccttcaccag aagagcaccc ccgtaaacca attgatcgat atattgccaa taagaaaaga
    11341 cattggcgta aaagatatcg atccctcgtg atgcattgcc gttgatactg ctattcatgg
    11401 ccgaaagcgc cacgacttta acttcttccg tttgagtgac attcatgttc ggcaatgtcg
    11461 ctttggatac ccgctctgct aaaggaacga cactggtatt gtatttcgta tctgtatctt
    11521 ttccaaaaga ccatgccaac gaatcctctg ggaaccaata agacgactcc ggttgattgt
    11581 ccattgcttt tttcaacata cttttggctg tttctcgata aacgacattt ccaattgtct
    11641 tcgcctcact ttggcgataa tcgatggtcg ttgccactgc caacactaac aacgatgctc
    11701 caattttact gattttcctt gtcacacttc ttcctccttg ttttacataa acccgtcaac
    11761 attccattcc taaactcaaa caatgaccct tcaaaggtgg agatatagga acaaattaaa
    11821 gaaatcatgc aataaatcca agaaggttgg ttttagaaca aaaaaagagt ctcctttttg
    11881 gggagactct ttgttaacta tctaaaaagg ttggctcttc tcacttcact cataatgtgt
    11941 ccaggcagaa taaatttcta caactttatt ttcctcatca actttgtaaa ccactcgatg
    12001 ctgtctattc agcctgcgtg agtatctgcc ttcatgtgta ggtctcagct tttcaaaaga
    12061 ctgcgtgggc atatatggat cttccttcaa tgtctgaatc acttcttcaa attgtttctt
    12121 taaattcgtt tgcttgattt ttcttaaatc cactttcgca gagtttttga ttgcgactgt
    12181 atagttactc atcaaagagc ttcccaatca atatcatcga tattcgtaaa gctactgtcg
    12241 tctttttcgc ggtctcgtac tttatctaaa gtacctgttt gctcaagaaa aagggtttct
    12301 tgaatcgctc tccagtcttc cagcccaatc aatacagcat tgtttccact acgatcacta
    12361 ataatctcga tttcagtatg attttcattt acctctttca ataactgata aaaattttta
    12421 cgagcagtgc taggattcat cgttgtatta gccattgtct ctcctccttt ttctgatata
    12481 attatagcgt acttgattaa gtacgtcaaa aaaatacaat aaagcactta aattcagcaa
    12541 acttttcctc ttttttacat agagaaaaag accccttttt agggaggatc tttgtccacg
    12601 ctcacttctt actcctttac gactagcggt agccaaacgt aaagtaattg gtaacaacgt
    12661 atcatcttta gcaaaggtcg aatccaaagc ctgtgagtct atgcaacaac accaatacac
    12721 gaaggttcct cgttgcagtg tgacttgatt gatatcttga ataacctgag gatacgcacg
    12781 aaagatttgt aacggttatt caaaactgat tttggccgtc aagtcttcta ctgcctcaaa
    12841 aatgattgcc tctttcgtct cctgtcattc atgttcacta tcgatttgat acttgcttag
    12901 accgttgggt ttgtgaatca ccaattaaaa atggcactga gttatggtaa caaaaaatgg
    12961 tcacttgacg aggctgagat tcctacttat gaagacacat ggcacttaag agtcgaggta
    13021 aaggaagaag ctgaaaatat aggaggctgt acattatttg gcaaaggatt cggaaattat
    13081 gatcaagaca tcaaaatcaa aatgttttac agctaaatag gaagaataat aacatttcat
    13141 acgctgttcc catctttaat ggcttataga gggcaccttt agctgagaat aagacaaaac
    13201 agaccctcta gcttcaatta agagctagag ggtctgtttt tgattttaaa acttggttta
    13261 tttcttcaaa tggtagctat aggtgctgac aacgatattt tcacgccaag acaatcgcaa
    13321 tcttaaccgc aaacttgttt ggttatggag aatattctgc cagcgtctgt ttggtacaaa
    13381 ttgaggaatc aacacagtag ttgtatagtt ttgtttcgct gcatttttac ttacgatatc
    13441 tacataacgg ataattggat tttcaatcga gcgataagaa gagtggacga ttgaaaaacg
    13501 gacatctggg aaatgctttt taaattctgc ttcgatttct ttttcttttt cgacatcttc
    13561 gtctagcgaa acatgcatcg caacgacata atcaccgatc gaacgtgcgt aattcaacgc
    13621 accgatattt acccgtgtca cattcccgac taatacaaga acggtattcc cgtcataatc
    13681 atgtagtttt gcatcatttt ctaaacgaag ctgttctgct acttttttat aatgatcatg
    13741 gatcttataa aaaatgaaca taacgattgg cataatgatg aagaaaggcc aaatgtcccc
    13801 taaccgatag acaaacaaaa tcgcaatgat tgcatatgag atgaacgcac ccgtaatatt
    13861 ggcaatcgat ttgcttaacc atctttttcc ttcttttttc catttgacga ccatccctgt
    13921 ttgagataaa gcaaatggaa tgaacacgcc gatagagtat aacggaatca agcgttctgt
    13981 tgagccatgg aaaataaaca gcaatacgat cgaacctagc gctagtgtga taatcccatt
    14041 ggaatagcct agtcgatccc cacgatcttg atacatgtga ggcatgaatt tatcttttgc
    14101 cagattgtat gcaagaactg gaaaagcaga aaaaccggtg ttagcagcaa ctgctaaaat
    14161 cagcgctgta gcaaattgaa ggacgtaata caaaatacca tgtccaaaga cagctttacc
    14221 aatttgagac aaaacagtga cttctttttc aggaacgatc ccataccaat agttgataaa
    14281 tgtgatcccc acgaagaaga aacctaagat caatgccatc atcgtcaatg tagcagcggc
    14341 attttttgct cttggcttct taaagaatgg tacagcatta ctaatcgcct ccaccccagt
    14401 taaagaggaa gaaccagaag aaaatgctcg taaaatcaaa gcgatagaaa cacccggcac
    14461 agccgctcct ggaagtgcag tagcttgcaa tggcacagca cctgtcacga tattatataa
    14521 accaacaacg atcagtaacg agatcacaag gataaatgtg tacaccggaa ataataggaa
    14581 cgatgcagat tccctcagcc cacgaagatt cagcatcatc agtagcaaaa cgattgtcac
    14641 agaaatagcg acttgatgac cgtagagtgc tggcaccgct gatgtgatcg cttcagcacc
    14701 tgctgagaca gatactgcta ctgtcaacat gtagtcgatc aataaagagc caccggaaat
    14761 caagccagca ttctttccaa gattctcact gctgacgaca tatgctcccc cgccatgagg
    14821 ataagcatgg atgatttgac gatatgatag cgttaatgaa atcaataaaa taataacaaa
    14881 agcggcaatg ggtagcgaat accagatggc tgcagcagac aaagcgacta aaacgacaac
    14941 gatttgttcc gtaccatatg caatagatga taacgcatca gacgacaata atgccaaagc
    15001 agcaaatcgg gttaatttgt gctcatcatt ttcagcagat tttaaaggct tacctactaa
    15061 caaccttttt aagtaatcca atttctaaac tcctttacac acaattttac ttcaagtttt
    15121 atag
//
LOCUS       DAZKOK010000069        15072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000069 DAZKOK010000000
VERSION     DAZKOK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.069"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG90W_001831"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG90W_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG90W_001832"
     CDS             706..1734
                     /locus_tag="ACG90W_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0478185.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG90W_001833"
     CDS             1731..2489
                     /locus_tag="ACG90W_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0478186.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG90W_001834"
     CDS             complement(2575..3315)
                     /locus_tag="ACG90W_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0478187.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG90W_001835"
     CDS             3376..4629
                     /locus_tag="ACG90W_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0478188.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG90W_001836"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG90W_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG90W_001837"
     CDS             5300..5626
                     /locus_tag="ACG90W_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0478189.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG90W_001838"
     CDS             5694..6407
                     /locus_tag="ACG90W_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0478190.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG90W_001839"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG90W_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG90W_001840"
     CDS             7690..9555
                     /locus_tag="ACG90W_001840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0478191.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG90W_001841"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG90W_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG90W_001842"
     CDS             11120..11821
                     /locus_tag="ACG90W_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478192.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG90W_001843"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG90W_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG90W_001844"
     CDS             complement(12857..13066)
                     /locus_tag="ACG90W_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0478193.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG90W_001845"
     CDS             13307..13882
                     /locus_tag="ACG90W_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0478194.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG90W_001846"
     CDS             13882..14322
                     /locus_tag="ACG90W_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0478195.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG90W_001847"
     CDS             14338..15039
                     /locus_tag="ACG90W_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0478196.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5083 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tt
//
LOCUS       DAZKOK010000070        14672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000070 DAZKOK010000000
VERSION     DAZKOK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.070"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG90W_001848"
     CDS             complement(11..1942)
                     /locus_tag="ACG90W_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0478197.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG90W_001849"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG90W_001849"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0478198.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG90W_001850"
     CDS             complement(2871..3623)
                     /locus_tag="ACG90W_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0478199.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG90W_001851"
     CDS             complement(3782..3991)
                     /locus_tag="ACG90W_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478200.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG90W_001852"
     CDS             complement(4002..5330)
                     /locus_tag="ACG90W_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478201.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG90W_001853"
     CDS             5466..5621
                     /locus_tag="ACG90W_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0478202.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG90W_001854"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG90W_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0478203.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG90W_001855"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG90W_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0478204.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG90W_001856"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG90W_001856"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0478205.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG90W_001857"
     CDS             complement(9305..10237)
                     /locus_tag="ACG90W_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478206.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG90W_001858"
     CDS             complement(10394..11053)
                     /locus_tag="ACG90W_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0478207.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG90W_001859"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG90W_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0478208.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG90W_001860"
     CDS             complement(12094..12384)
                     /locus_tag="ACG90W_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478209.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG90W_001861"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG90W_001861"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0478210.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         4175 a         3105 c         2552 g         4840 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct ta
//
LOCUS       DAZKOK010000071        14604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000071 DAZKOK010000000
VERSION     DAZKOK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.071"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(172..738)
                     /locus_tag="ACG90W_001862"
     CDS             complement(172..738)
                     /locus_tag="ACG90W_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0478211.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(738..1694)
                     /locus_tag="ACG90W_001863"
     CDS             complement(738..1694)
                     /locus_tag="ACG90W_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0478212.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1694..2350)
                     /locus_tag="ACG90W_001864"
     CDS             complement(1694..2350)
                     /locus_tag="ACG90W_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0478213.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2720..4738
                     /locus_tag="ACG90W_001865"
     CDS             2720..4738
                     /locus_tag="ACG90W_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0478214.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4808..5014
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5165..7579
                     /gene="leuS"
                     /locus_tag="ACG90W_001866"
     CDS             5165..7579
                     /gene="leuS"
                     /locus_tag="ACG90W_001866"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0478215.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7927..9480
                     /locus_tag="ACG90W_001867"
     CDS             7927..9480
                     /locus_tag="ACG90W_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0478216.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9861..11645)
                     /locus_tag="ACG90W_001868"
     CDS             complement(9861..11645)
                     /locus_tag="ACG90W_001868"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478217.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11660..13381)
                     /locus_tag="ACG90W_001869"
     CDS             complement(11660..13381)
                     /locus_tag="ACG90W_001869"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478218.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13481..14242
                     /locus_tag="ACG90W_001870"
     CDS             13481..14242
                     /locus_tag="ACG90W_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0478219.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4741 a         2700 c         2910 g         4253 t
ORIGIN      
        1 ataagaacag ccaatcaaaa acagcttctc atgtttttga tttggttgga cttattaccg
       61 cagatataag cttttgagca ccgtgaataa agaggttatg aaatcaacgc tctaacctga
      121 gtattagaga aaaaaacgca aaacatatca tttatgtttt gcgtttttta tttatctttt
      181 tttctcaata caatataaaa taggcggctg gttcttttgg ttgataaacc gataattcag
      241 cacattatat gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat ccagttcttt
      301 ttctccacct tcatgaccat agtagacgac aaggatcaat cgtccgcgag gcacaagtcg
      361 tttcaatatt tcttccatag ctgtgcgtgt agtctctggc atagtgatga ttgctttatc
      421 actttttggg agatacccta gattgaaaat ccctgctttg agattttgtt gttcagctat
      481 cacttctcct aacgtttcgt ggccttgtag aaacaatgag acacgttctt ctaactgacg
      541 ctgttctagc ctttcacgag tagcatcgat tgcttgttgc tggatatcaa aactatagac
      601 atgaccagtt tttccgatgt gttctgctag aaaaaccgta tcgtggccat tgcccatcgt
      661 tgcatctacg acgtgatccc ctggttgcaa gatttcttgc agtaatgtat ggctgaaatg
      721 caaagcagtt tgcaacatca gacgctcacc tttttccgta catcatattt tccttggaag
      781 ctatctctgc gaagaagttc ttcatcaatt gcattcaaga cttcccattt tttcaagctc
      841 cacattggtc caatcaagga atcccacggc gcatcccctg tcaaacgatg gatgatgatc
      901 tcttttggta tcatttccag ctggtcgcaa atgacattga catagtcagg acggctcatt
      961 aattgaaggc gaccttcatg atagtcccgc aacattctcg ttttgcgcat cagatgcatc
     1021 aaatggagct tgattccttg gatatctgaa tctaaaatgg tgcgtctgac attttctttc
     1081 atcatttcta aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac acggatatta
     1141 tgtttgcgta atttagccac gccatctaaa taagtttgat aatcatgtgc tcggttgatc
     1201 agttttgaag tttcttcaaa agttgtttgc aatccaagtt ctacccataa atacatccgc
     1261 tcgttcagtt cagcaaggta atcaactact tcatctggca gacaatccgg tcgtgtaccg
     1321 actgataatc ctacgacacc tggtaaattg actacttgct caaatcgttc tttgattatt
     1381 tcaactggcg catgggtgtt tgtaaaattt tggaaataaa cgatgtattg ttggacatga
     1441 ggccattttt gatgcatcat gtcgatttct ttctggaatt ggattggaag agggtcttca
     1501 ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa aagtacaacc gccatgggca
     1561 acagtcccat ctcgattcgg acagtcaaat cctccatcga tcggcacctt gaaaattttc
     1621 ccgccaaact catttcgtaa agcgtaattc caagaatgat atcttttatt tccttctgaa
     1681 taaggaaaag tcattaaaag tccttctttc tgtaagagtt atctatttgt tttctttttt
     1741 ctctatacac tggataagtc aggcacagcc aagaaagact gagtaagtaa ccgccgatga
     1801 tatcagtagg aaaatgaacg cccagataaa tgcggcttat cccgacactt actataataa
     1861 acatcatcag caattgaagc aatgtgcgta aggttctttt ttgaacaaaa gctggaagca
     1921 gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc atgtccacta gggaaactgt
     1981 aacttgtttc aactactaag tgcgccaatg aaggacgctc tcgcatgaaa aatagtttga
     2041 tcaccgggtt gatgattcca gcaattacaa taaatccaga aacgaaccaa aatgtttctg
     2101 cccttttttt cttataccac atccataaag ccgcacaagc aaataaaatg atgacggtaa
     2161 gaggattacc gaatttagtc ataaagagtt ggtagctgtt cagatcagga taagaagctc
     2221 tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg aactttacga
     2281 tccagcccaa aatgaaaaac aggacaagaa agaagctacc agccacctga aatgatgttt
     2341 tatatttcat acaattcctc ctttgaagca atgacttatt atagcatttt tggtccagcg
     2401 aaaaaaacgg taaagagata aaaccaatgt atgattcctc tttgaataac caatattctt
     2461 ataaaaaaaa taactgtaat cggaaagaca ttttttggta atgcaaatga aatgttaggg
     2521 aaataaaatc agtgtttttc gaaatccttt taaacaagaa cgtagacgta ggaagaactt
     2581 gaaaatgaac agaagttgtt cattacggta aattacaata tgattacaaa aaacaacaaa
     2641 gctcttaaat aacttttaat aaatattaag agaagttata attattaata atagataaat
     2701 tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac gtttagaaca
     2761 gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg cgatggtagg
     2821 ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg aaacacctgt
     2881 tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta cttatgctca
     2941 accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag cagttgtaga
     3001 aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt ttgggataaa
     3061 aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa ataatcaatg
     3121 ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt tcaatgacaa
     3181 tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg gtacttggaa
     3241 gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt atgcgactga
     3301 tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga cccaatatga
     3361 tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata atactggaag
     3421 tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt cagcaacttc
     3481 gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata aattcggtat
     3541 cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt atccaggaca
     3601 aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt ccggtaacaa
     3661 taccaattct tcaggaaatg ctggtacatc aaacggagga caaacaactg gtgcgaaata
     3721 cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa ttacgatggc
     3781 tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac aacagctaat
     3841 cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata ctgggaatac
     3901 aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa cgaaatatac
     3961 agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta cgatggctca
     4021 gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac aactgactgt
     4081 ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa ccaatacaaa
     4141 taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata ccgtgaaagc
     4201 aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc agttaatcga
     4261 atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg ttaaaggtgg
     4321 aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa atacaccgaa
     4381 tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg tgaaagcggg
     4441 cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt taatcgaatg
     4501 gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga agaaaggttc
     4561 atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg tgaaatctgg
     4621 agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac taatcgaatg
     4681 gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag ctaaataaaa
     4741 tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta taaggctact
     4801 taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag agagcgcatg
     4861 gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct tgctgaatac
     4921 ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc actaaggtaa
     4981 aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt ataggatttt
     5041 tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat aaacagattc
     5101 tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa caggaggaca
     5161 gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact gggcaaaaac
     5221 gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac tggatatgtt
     5281 tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata cggcaacgga
     5341 tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa tggggtggga
     5401 tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc cagctgagtt
     5461 tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat ttagttatga
     5521 ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac aatggatctt
     5581 tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa actgggtacc
     5641 tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg aacgtggtgg
     5701 ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg cttatgctga
     5761 tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag aaatgcaaag
     5821 aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg gtacggataa
     5881 atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt actcggttct
     5941 tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag ctgctgtaga
     6001 agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc ttgcaaaaga
     6061 aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca aggaaatccc
     6121 aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca tggctgttcc
     6181 agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa ttcttccagt
     6241 tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc acatcaactc
     6301 tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg aatggctaga
     6361 agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc tattctcccg
     6421 tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa cagtcacagc
     6481 tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca agccaagcgg
     6541 tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg atcctgtaac
     6601 aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa gctcatggta
     6661 ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg aaaagttgaa
     6721 gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac ttcatttact
     6781 atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta caaaagaacc
     6841 attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa aaatgtcaaa
     6901 atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg cagatacact
     6961 gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga gcgaaaatgg
     7021 cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg atgagaacaa
     7081 taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag tgtatcatca
     7141 aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag caatctccca
     7201 attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg aatatatcga
     7261 aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac tatgggcaat
     7321 ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg aagcagcact
     7381 tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg cgaaaagcaa
     7441 tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca atgaaatagt
     7501 gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa acaagttagt
     7561 caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt gtgcaaaatc
     7621 aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc aaattgatta
     7681 attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat gtctttttct
     7741 tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt aatcttagta
     7801 ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata aattttttat
     7861 tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg agaaaaggag
     7921 agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca aaaaattatc
     7981 gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt agtatttttc
     8041 tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag tgcagtagtc
     8101 cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg gggagttggc
     8161 ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg ttaccatcaa
     8221 ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt acataataaa
     8281 ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag tcaagctttt
     8341 cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt acctatctac
     8401 ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt actagaaaac
     8461 gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac taagatgagt
     8521 acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta tgggagatac
     8581 actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat gaactcaact
     8641 aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa gcaaaaggta
     8701 aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca ctctctctat
     8761 atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca atggttgcgt
     8821 tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat ggacaacaca
     8881 tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga tgtacgtctg
     8941 ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca atcagttcag
     9001 ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat ctgtacatca
     9061 gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg cggaatcact
     9121 tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag cttcgttaca
     9181 acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa cggaggacgc
     9241 tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg gatagatctt
     9301 tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac aaaattttat
     9361 ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt ccgttcaact
     9421 gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca aggagattaa
     9481 agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa ttcaaaaaac
     9541 agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag agcaacaaga
     9601 agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg ccgataggtt
     9661 gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg tcactactta
     9721 ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt tgtgtttcta
     9781 gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata catgatcgat
     9841 agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag actggcataa
     9901 agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc gccattcttc
     9961 attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat cacaaaactt
    10021 gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt tcgtgtatct
    10081 acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat gactcgagca
    10141 attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga aatgatcgta
    10201 tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc agccgcttcg
    10261 ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc taccgttcct
    10321 tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc tccacgtgaa
    10381 agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc ataaaaacgc
    10441 atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat cgctaccgtt
    10501 tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga tgtgtaacca
    10561 aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct tgcatctgga
    10621 aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat tgctgccatt
    10681 gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga aatctgattg
    10741 atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag aacaagcata
    10801 gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat ggccggatag
    10861 atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt atttacttcg
    10921 tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag attaccagct
    10981 aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg ttcagaaatc
    11041 tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat cagcaataag
    11101 acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc agctattgtt
    11161 acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag ttgattgaga
    11221 ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa cgacatcggt
    11281 aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt ttcgcttaac
    11341 gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga acagccgatc
    11401 agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag ctgtccaata
    11461 cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat caagggcatg
    11521 gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa atatttatgt
    11581 tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt ttttttcttt
    11641 tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga taagtttggc
    11701 aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca tctaaaacaa
    11761 tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg agagttttct
    11821 ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag tctaaagcgg
    11881 agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga gcaatacata
    11941 tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag ctgtccaatc
    12001 cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct ttagacaact
    12061 cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc ccgctgaata
    12121 agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga gtcatttgag
    12181 taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt ctcaagagaa
    12241 gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt gtttttcctt
    12301 taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta tatttaaatg
    12361 tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct actgttttga
    12421 tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt cgtaatgagg
    12481 caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa gcaagtgtca
    12541 gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct tctgctacaa
    12601 gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa aaagcaattg
    12661 gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag tcagcaaagc
    12721 tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc atattaatcc
    12781 cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg atactcaaag
    12841 ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa acaacagaga
    12901 tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg gtgaaaacgc
    12961 aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc tggacattat
    13021 cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg atatccttca
    13081 ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca aacctagcgg
    13141 aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga aacgctacaa
    13201 gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct atacctgtgt
    13261 cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct aaacagatca
    13321 tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt aagatagaca
    13381 tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta acttgaaggt
    13441 caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa tgtatacgat
    13501 tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt gggatgaaca
    13561 aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg atatcgatga
    13621 tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga tcaaacagat
    13681 gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg aaacagaaag
    13741 aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa gggagcaaaa
    13801 aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag aagagatccc
    13861 ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa aatttctacc
    13921 taactattca agctttggga tgatgagtat gccagcttcc agttcacaga cgatgtatgg
    13981 attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt gggagaaaaa
    14041 agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg cggaacgtaa
    14101 taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag gagaagtgat
    14161 cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat attatcatgc
    14221 atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa tgatggttgt
    14281 tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg gattattagt
    14341 cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat ggaatggttg
    14401 caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga aaaataattg
    14461 tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa aattcatatt
    14521 gttatgagtg agattgtatc agatgagaat gataaattaa ataggtgcat aaaagcatta
    14581 cacttaaaat acattgttgc ttac
//
LOCUS       DAZKOK010000072        14549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000072 DAZKOK010000000
VERSION     DAZKOK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.072"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG90W_001871"
     CDS             4..816
                     /locus_tag="ACG90W_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0478220.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG90W_001872"
     CDS             813..1613
                     /locus_tag="ACG90W_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0478221.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG90W_001873"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG90W_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0478222.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG90W_001874"
     CDS             3110..4819
                     /locus_tag="ACG90W_001874"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0478223.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG90W_001875"
     CDS             5031..9389
                     /locus_tag="ACG90W_001875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0478224.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG90W_001876"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG90W_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0478225.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG90W_001877"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG90W_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0478226.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG90W_001878"
     CDS             11227..11520
                     /locus_tag="ACG90W_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0478227.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG90W_001879"
     CDS             11517..11828
                     /locus_tag="ACG90W_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0478228.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG90W_001880"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG90W_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0478229.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG90W_001881"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG90W_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0478230.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DAZKOK010000073        14280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000073 DAZKOK010000000
VERSION     DAZKOK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.073"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..428)
                     /locus_tag="ACG90W_001882"
     CDS             complement(<1..428)
                     /locus_tag="ACG90W_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0478231.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEG"
     gene            complement(582..1112)
                     /locus_tag="ACG90W_001883"
     CDS             complement(582..1112)
                     /locus_tag="ACG90W_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0478232.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1211..1561)
                     /locus_tag="ACG90W_001884"
     CDS             complement(1211..1561)
                     /locus_tag="ACG90W_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0478233.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1718..3247)
                     /gene="ahpF"
                     /locus_tag="ACG90W_001885"
     CDS             complement(1718..3247)
                     /gene="ahpF"
                     /locus_tag="ACG90W_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0478234.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3257..3820)
                     /gene="ahpC"
                     /locus_tag="ACG90W_001886"
     CDS             complement(3257..3820)
                     /gene="ahpC"
                     /locus_tag="ACG90W_001886"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0478235.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3964..4626)
                     /locus_tag="ACG90W_001887"
     CDS             complement(3964..4626)
                     /locus_tag="ACG90W_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478236.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4810..6456
                     /locus_tag="ACG90W_001888"
     CDS             4810..6456
                     /locus_tag="ACG90W_001888"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0478237.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            6447..7529
                     /gene="uxuA"
                     /locus_tag="ACG90W_001889"
     CDS             6447..7529
                     /gene="uxuA"
                     /locus_tag="ACG90W_001889"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0478238.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7637..9190)
                     /locus_tag="ACG90W_001890"
                     /pseudo
     CDS             complement(7637..9190)
                     /locus_tag="ACG90W_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9338..10480
                     /locus_tag="ACG90W_001891"
     CDS             9338..10480
                     /locus_tag="ACG90W_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0478239.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEETETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPTLSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHLTPYSQRQTIEQDSAID
                     SGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNKSKPEIT
                     PGEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQ
                     GIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTF
                     ASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(10606..11685)
                     /locus_tag="ACG90W_001892"
     CDS             complement(10606..11685)
                     /locus_tag="ACG90W_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0478240.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11794..12234)
                     /locus_tag="ACG90W_001893"
     CDS             complement(11794..12234)
                     /locus_tag="ACG90W_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0478241.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(12291..12701)
                     /locus_tag="ACG90W_001894"
     CDS             complement(12291..12701)
                     /locus_tag="ACG90W_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0478242.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(12882..13316)
                     /locus_tag="ACG90W_001895"
     CDS             complement(12882..13316)
                     /locus_tag="ACG90W_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0478243.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(13416..14264)
                     /locus_tag="ACG90W_001896"
     CDS             complement(13416..14264)
                     /locus_tag="ACG90W_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0478244.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         4833 a         2672 c         2496 g         4279 t
ORIGIN      
        1 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
       61 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
      121 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
      181 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
      241 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
      301 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
      361 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
      421 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
      481 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
      541 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
      601 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
      661 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
      721 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
      781 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
      841 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
      901 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
      961 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     1021 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     1081 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     1141 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     1201 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     1261 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     1321 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     1381 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     1441 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     1501 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     1561 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     1621 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     1681 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     1741 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     1801 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     1861 tcactatttc atttcttgga ttcatttcga tcgttctatc tagccaatcc gtattaggga
     1921 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     1981 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     2041 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     2101 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     2161 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     2221 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     2281 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     2341 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     2401 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     2461 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     2521 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     2581 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
     2641 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     2701 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     2761 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     2821 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     2881 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     2941 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     3001 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     3061 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     3121 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     3181 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     3241 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     3301 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     3361 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     3421 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     3481 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     3541 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     3601 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     3661 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     3721 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     3781 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     3841 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     3901 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     3961 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     4021 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     4081 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     4141 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     4201 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     4261 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     4321 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     4381 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     4441 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     4501 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     4561 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     4621 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     4681 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     4741 catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     4801 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     4861 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     4921 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     4981 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     5041 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     5101 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     5161 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     5221 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     5281 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     5341 attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     5401 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     5461 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     5521 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     5581 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     5641 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     5701 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     5761 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     5821 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     5881 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     5941 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     6001 ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     6061 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     6121 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     6181 ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca taagctttcc
     6241 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     6301 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
     6361 gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt acaggattac
     6421 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
     6481 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
     6541 cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa gaactaaaag
     6601 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
     6661 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
     6721 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
     6781 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
     6841 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
     6901 aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag
     6961 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
     7021 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
     7081 actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat tggttgtgtc
     7141 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
     7201 cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga atcccatttg
     7261 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccacataaat
     7321 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
     7381 acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
     7441 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
     7501 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
     7561 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
     7621 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
     7681 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
     7741 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
     7801 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
     7861 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
     7921 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
     7981 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
     8041 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaata
     8101 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
     8161 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
     8221 caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt gaataaagaa
     8281 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
     8341 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
     8401 ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca atatattcat
     8461 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
     8521 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
     8581 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
     8641 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
     8701 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtactatg
     8761 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
     8821 gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
     8881 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
     8941 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
     9001 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
     9061 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
     9121 gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta atcgcttctt
     9181 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
     9241 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
     9301 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
     9361 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
     9421 gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
     9481 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
     9541 catcaaagca aaaattgact attcgggaga cttagcacct atactacaat cagctgttac
     9601 atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt tgactatcca
     9661 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
     9721 gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa cgccgtatag
     9781 tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc ttgttgaaga
     9841 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
     9901 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
     9961 gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg taactccaga
    10021 accagatacg gattctggaa accaaacagt accggaaaca aatccagaca cagataatga
    10081 aactgaaaat ccagaaaaac tagaggtaga tccagaagaa aaaccagatg tgactccaga
    10141 accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa aaacaattca
    10201 agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa taaatgaaga
    10261 gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag
    10321 tagcaaggat acaacctttg ctagtcctga aacgaaaaat aaacagctac caaaatccgg
    10381 agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta tagcaacaat
    10441 gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata atgtctgtga
    10501 acgcattttt caaataaaaa gaaatatcca gaaaacgacc ccaaaatacc agattgaaat
    10561 ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag ggatatctgt
    10621 ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa agaaggtaaa
    10681 gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa caagtaagac
    10741 cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg gattcagcac
    10801 ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa catctcggat
    10861 gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa cacctgctac
    10921 tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat gaaagccaaa
    10981 tacagccaag gcgattgtag tgagcaacgt gtttaaaaga gcgataacaa attgagcttc
    11041 taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat cttgaaagaa
    11101 ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt tcttttcaat
    11161 cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt ttcctatatc
    11221 ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa aatcgtttcg
    11281 ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct gatgctcgta
    11341 aaaatcaata acggactgga tcatatcaaa tgtttgatga ataagcatcg ggatatactt
    11401 tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag ctaaaacacc
    11461 tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca aataagtaaa
    11521 gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa caaaaacgac
    11581 aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct gataaacggt
    11641 caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat gatggaatat
    11701 gaaaataaga aagagaaaga gcaaaaagaa aaaccgctaa atgacgttat cttatgagtt
    11761 ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt cttgatgaaa
    11821 attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct gttcaagtgg
    11881 aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg cagcaccttg
    11941 aggagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac gattaatata
    12001 atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca gacacaaatc
    12061 atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca tcacaagata
    12121 ctgggtatag gtaagattca gcggcttgag gagggaagtg tagtgtctaa tgatttcctt
    12181 agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt ccatttttcc
    12241 acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt ctattcacta
    12301 actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg acacacttta
    12361 tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat agatccttca
    12421 atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac ttgtgcactg
    12481 acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc actgttaaaa
    12541 catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc gacgcgttta
    12601 ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgaacttc tccagagcgt
    12661 ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc acctttaatt
    12721 atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa atggattggc
    12781 ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag caaacatata
    12841 caaaaaaata tcagagacaa aaactaatct catcataaac attacatata tttctcaaaa
    12901 agttgttttc tttcttcaaa acttacttca cgatggacct gatgaagcat ccataaataa
    12961 ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc tgtcatttct
    13021 tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat atcagagacg
    13081 atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa accaagagac
    13141 gtgttttcat ccagcatatg gaatctcgtg ccatacaagg taaaaatgac ttcattattg
    13201 cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt ttgatacagt
    13261 tctagtgctt tcaaactatc atttaataca aaatctattt ccgttccttt atgcataaag
    13321 atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca gttaaagaaa
    13381 acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg ataagtaaat
    13441 ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg atcgatttct
    13501 tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa taaaacctct
    13561 tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg tccaatctca
    13621 gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat aacagatttc
    13681 tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc aatagataaa
    13741 atagcataaa gaaactcttc tattccaaaa acaaacagat taagagaaac aacgaatgcg
    13801 tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa aatgagggga
    13861 gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac tcctaaagca
    13921 aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat tgtttctgga
    13981 actattgcaa tgattaaagg aaaaaacaag cttccgaaaa gtactttaaa aaagggtttt
    14041 ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa aataactata
    14101 gctcggtcaa atcctaaaac agcttccagt aatattccta gaccgctgac acctccagca
    14161 gcaatatgat gaggccctaa aaacatattg atagcgactc ctaatatgaa gagggcaaaa
    14221 ataatcttaa tgtatgtcca tataaattgt ctcgtttctt tcatttatcc acttccttaa
//
LOCUS       DAZKOK010000074        14186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000074 DAZKOK010000000
VERSION     DAZKOK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.074"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..901)
                     /locus_tag="ACG90W_001897"
     CDS             complement(77..901)
                     /locus_tag="ACG90W_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0478245.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(874..1554)
                     /locus_tag="ACG90W_001898"
     CDS             complement(874..1554)
                     /locus_tag="ACG90W_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478246.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1577..2515)
                     /locus_tag="ACG90W_001899"
     CDS             complement(1577..2515)
                     /locus_tag="ACG90W_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0478247.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2760..3611)
                     /gene="ispE"
                     /locus_tag="ACG90W_001900"
     CDS             complement(2760..3611)
                     /gene="ispE"
                     /locus_tag="ACG90W_001900"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0478248.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3765..6338)
                     /gene="mprF"
                     /locus_tag="ACG90W_001901"
     CDS             complement(3765..6338)
                     /gene="mprF"
                     /locus_tag="ACG90W_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0478249.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6479..7285
                     /gene="proC"
                     /locus_tag="ACG90W_001902"
     CDS             6479..7285
                     /gene="proC"
                     /locus_tag="ACG90W_001902"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0478250.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7364..8920)
                     /gene="rny"
                     /locus_tag="ACG90W_001903"
     CDS             complement(7364..8920)
                     /gene="rny"
                     /locus_tag="ACG90W_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0478251.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9331..10380)
                     /gene="recA"
                     /locus_tag="ACG90W_001904"
     CDS             complement(9331..10380)
                     /gene="recA"
                     /locus_tag="ACG90W_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0478252.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10489..11730)
                     /locus_tag="ACG90W_001905"
     CDS             complement(10489..11730)
                     /locus_tag="ACG90W_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0478253.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11970..12548)
                     /gene="pgsA"
                     /locus_tag="ACG90W_001906"
     CDS             complement(11970..12548)
                     /gene="pgsA"
                     /locus_tag="ACG90W_001906"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0478254.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12757..13680)
                     /locus_tag="ACG90W_001907"
     CDS             complement(12757..13680)
                     /locus_tag="ACG90W_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0478255.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
BASE COUNT         4067 a         3152 c         2477 g         4490 t
ORIGIN      
        1 tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc
       61 ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga
      121 tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat
      181 ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga
      241 tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca
      301 tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag
      361 taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat
      421 cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg
      481 caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa
      541 acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg
      601 ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga
      661 tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg
      721 taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg
      781 tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc
      841 cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca
      901 ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc
      961 atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag
     1021 acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa
     1081 taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga
     1141 caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg
     1201 tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc
     1261 tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg
     1321 aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat
     1381 tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc
     1441 attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag
     1501 tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca
     1561 acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga
     1621 cagaaatata attttcgcct gctttgattt gttcattagt caagctttct aaaggattca
     1681 acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat
     1741 tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta
     1801 agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt
     1861 cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg
     1921 catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat
     1981 cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa
     2041 tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat
     2101 gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac
     2161 ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta
     2221 acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa
     2281 tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca
     2341 aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa
     2401 aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg
     2461 ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata
     2521 tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa
     2581 aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg
     2641 attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa
     2701 ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt
     2761 tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact
     2821 ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc
     2881 aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc
     2941 cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct
     3001 tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct
     3061 acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag
     3121 cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta
     3181 cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct
     3241 tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct
     3301 gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca
     3361 ccggttttga tattgtattt attttttagc aataaagcag cttgataaac attgttccgt
     3421 tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct
     3481 tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac
     3541 ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca
     3601 atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga
     3661 aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt
     3721 tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc
     3781 acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc
     3841 atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc ttcccaatga
     3901 tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa
     3961 ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta
     4021 gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac
     4081 agattggcta acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa
     4141 tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga
     4201 tctttacgga caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca
     4261 gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga
     4321 tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt
     4381 tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct
     4441 gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct
     4501 gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc
     4561 gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa gcatttgttt
     4621 cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta ataaaaataa
     4681 ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa
     4741 cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat
     4801 tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac
     4861 acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac taaaaagcgt
     4921 ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca
     4981 aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac
     5041 cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg
     5101 attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact
     5161 tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc
     5221 tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac
     5281 agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac agcacttacg
     5341 agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg
     5401 attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa
     5461 taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac agacaattga
     5521 ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac catcgtcagc
     5581 attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt
     5641 ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat
     5701 gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt ctttctcttt
     5761 agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa
     5821 tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac
     5881 catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt
     5941 tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac cacaccacca
     6001 aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt
     6061 tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac
     6121 atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt
     6181 tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga
     6241 tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc
     6301 tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta
     6361 tacttttgtg tcatacccca atgttatctt atttatttta atttttccac tttagacctg
     6421 ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat
     6481 gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa
     6541 aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca
     6601 aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca
     6661 atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact
     6721 aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat cattaataga
     6781 tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa
     6841 tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca
     6901 gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc
     6961 agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat
     7021 cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat
     7081 tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg
     7141 ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt
     7201 agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga
     7261 tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag
     7321 aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag
     7381 ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt
     7441 cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg
     7501 gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg
     7561 cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt
     7621 cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga
     7681 taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt
     7741 tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat
     7801 ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt
     7861 cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga
     7921 cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat
     7981 gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat
     8041 ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat
     8101 cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg
     8161 aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa
     8221 gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa
     8281 gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta
     8341 atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt
     8401 ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc
     8461 gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca
     8521 ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt
     8581 ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt
     8641 gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct
     8701 cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt
     8761 tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg
     8821 caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga
     8881 caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat
     8941 ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat
     9001 gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc
     9061 gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt
     9121 tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa acttttctat
     9181 taaattattc tttgtagatg aacagatatt tttattttat gtacccactc gtataattaa
     9241 ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt
     9301 cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg
     9361 tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag
     9421 agcagaaact tctgccatca tttcaggatg attagccata tagattttag cgttttcccg
     9481 tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc
     9541 tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac
     9601 atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt
     9661 acggttacca acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg
     9721 gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat tcgcaaacat
     9781 cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat
     9841 cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga
     9901 gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac
     9961 tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc
    10021 tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg
    10081 cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc
    10141 tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat
    10201 gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact
    10261 tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc
    10321 atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa
    10381 tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct
    10441 tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa
    10501 aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg
    10561 atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca
    10621 tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac
    10681 actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt
    10741 gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt
    10801 ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc
    10861 taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt
    10921 ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa
    10981 atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc
    11041 ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg
    11101 tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga
    11161 ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg
    11221 ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc
    11281 gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt
    11341 acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt
    11401 catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg
    11461 tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg
    11521 tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc
    11581 ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc
    11641 agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc
    11701 tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct
    11761 gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac
    11821 agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata
    11881 tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc
    11941 aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt
    12001 ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt
    12061 tgatctactg gaataccgat caggctgaat gggatattat tgataaacag caagataata
    12121 gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca
    12181 tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct
    12241 actacccaac caggggcctt tccttggtca accaacatga taaaagcagt cataactagc
    12301 attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg
    12361 gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact
    12421 agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa
    12481 cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt
    12541 aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac
    12601 cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc
    12661 tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac
    12721 ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag
    12781 gttcacattt tttggactct gattgttttc ccctggatta actggtacgg attctccatt
    12841 cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc
    12901 cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt
    12961 gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt
    13021 ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc
    13081 cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg
    13141 cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc
    13201 tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac
    13261 gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact
    13321 tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg
    13381 acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc
    13441 tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc
    13501 aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt
    13561 aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc
    13621 tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat
    13681 ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc
    13741 cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca
    13801 ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc
    13861 tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc
    13921 ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga
    13981 tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac
    14041 caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc
    14101 agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc
    14161 gaaatatcac aaaaatttga gaagca
//
LOCUS       DAZKOK010000075        14104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000075 DAZKOK010000000
VERSION     DAZKOK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.075"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..2183
                     /gene="acm"
                     /locus_tag="ACG90W_001908"
     CDS             18..2183
                     /gene="acm"
                     /locus_tag="ACG90W_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0478256.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2455..2940)
                     /locus_tag="ACG90W_001909"
     CDS             complement(2455..2940)
                     /locus_tag="ACG90W_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0478257.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3030..3375
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3420..4061
                     /locus_tag="ACG90W_001910"
     CDS             3420..4061
                     /locus_tag="ACG90W_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0478258.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4080..4715
                     /locus_tag="ACG90W_001911"
     CDS             4080..4715
                     /locus_tag="ACG90W_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478259.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4733..5548
                     /locus_tag="ACG90W_001912"
     CDS             4733..5548
                     /locus_tag="ACG90W_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0478260.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5867..7303
                     /locus_tag="ACG90W_001913"
     CDS             5867..7303
                     /locus_tag="ACG90W_001913"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0478261.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7492..9939
                     /locus_tag="ACG90W_001914"
     CDS             7492..9939
                     /locus_tag="ACG90W_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0478262.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10169..11236
                     /gene="rlmN"
                     /locus_tag="ACG90W_001915"
     CDS             10169..11236
                     /gene="rlmN"
                     /locus_tag="ACG90W_001915"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0478263.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11226..11432
                     /locus_tag="ACG90W_001916"
     CDS             11226..11432
                     /locus_tag="ACG90W_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478264.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11429..12532)
                     /locus_tag="ACG90W_001917"
     CDS             complement(11429..12532)
                     /locus_tag="ACG90W_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0478265.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13085..13633
                     /gene="aac(6')"
                     /locus_tag="ACG90W_001918"
     CDS             13085..13633
                     /gene="aac(6')"
                     /locus_tag="ACG90W_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0478266.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(13881..14000)
                     /locus_tag="ACG90W_001919"
     CDS             complement(13881..14000)
                     /locus_tag="ACG90W_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0478267.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         4830 a         2342 c         2969 g         3963 t
ORIGIN      
        1 taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta
       61 ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca
      121 gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa
      181 aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga
      241 tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa
      301 tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt
      361 ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg
      421 ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg
      481 ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt
      541 attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata
      601 ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg
      661 gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg
      721 tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta
      781 atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta
      841 ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga
      901 atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca
      961 atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat
     1021 tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat
     1081 taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg
     1141 aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag
     1201 aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa
     1261 acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca
     1321 tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat
     1381 tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag
     1441 acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg
     1501 aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt
     1561 atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct
     1621 caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca
     1681 cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg
     1741 attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata
     1801 ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa
     1861 acagttatac accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga
     1921 aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc
     1981 aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag
     2041 ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa
     2101 aatctagttt tgctgtaatc attggtttat tgatagtaac tatctgtgct gggatattct
     2161 tacaaaaata taaaaaagtt tagttagaat ataatatttt ttgttaaata tgactgaaat
     2221 caaaagaata aaattccgat ttataatcga aaaaagaagc atttttatat atgcttcttt
     2281 tttcgtagaa tcaataaata tggatttggc taactatgga agaatactcg tattagtttt
     2341 cagcgttcat actagttgtt acattgtttg attggccaaa agaatttctg atatattttg
     2401 tgctaaataa agaaaaagac gcttgaattg ggaaataaga aaactactct atcattacaa
     2461 attttcataa actggcagat gttctaagta aacaagtatc ttttcaaagt cggaaaaatc
     2521 cataatcaac gtagtggtat tgatatttgg atgaaagcct acggtatctt gacgatcaat
     2581 agcagcatcg atcacgactt ggattttttt ctctttgtcg tgcatcaatg ctaatggagt
     2641 cactgtacct gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa caaaagataa
     2701 gcgcttttct tttaattgtt cagctagttg ttttaaatcg gcgtgctttt catcaggcaa
     2761 gatgacaaga tagatttgtt ttccttttct tgatttcaat aatagatttt ttacttgcgg
     2821 accaggtagg gaaaaaggga catttttgac tgacgtaatc gcaggatgat cgactttttg
     2881 aaaagagata cccagttttt ttagtagttc ataagcttct tgttcggttg cacgactcat
     2941 cgcgcatcac cctccttttt ttattagtgt atcataaaaa agagttgaca aatgaaaaat
     3001 aatcatgtaa attacacgta aagttaaaac aaatgacatc gaaaagaaga gtagccgtta
     3061 ggtgttttta gagagtctct ggttggtgga aagaggcagc agttaaccgt gaaacacatc
     3121 ttggagtcga ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt acagctgcgg
     3181 tcgttgctag atgaccagat aaggctggct tttccgtgag gagtcggtaa aaaaaggtgg
     3241 aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt tcatctgaac
     3301 ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg gaaccacgta
     3361 taatacgtcc tcttggcatt tgccaagagg atttttttat tttaaaggag gaaaagaaga
     3421 tggagtattt gattgaagtt atgccgcaat tattagtagg agcactcaca acattgaaat
     3481 tatttttatt taccttgatt ggctcattac cattagggat tgttttagca ttaggattgt
     3541 ctttgcattt taagccgcta cgctacttgc ttcaaacata tgtatgggtt atgcggggaa
     3601 caccgctgtt gttgcaactg atttttattt tttatgggtt gccgacgatt gggatcgttt
     3661 ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa ctatgcagct tattttgcag
     3721 agatctttcg tggagggata caatcgattc ctcaaggtca atatgaagca gcgcaagttt
     3781 tgcaattaag tccctttcaa acggtgacga aaatcgtcat cccacaagtg acaaagattg
     3841 ttttaccttc tgtagggaat gaagttatca acttggtcaa agattcttcc ttgatctata
     3901 tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc gacgcgactt
     3961 tactgccttt gctaggcgtg gcaatcatct atcttacatt gactggtatt ctaacaatca
     4021 taatgaaaca agttgaagga aaattaaatt attaccgcta actgatagga ggaagaaata
     4081 tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc aaagatttga
     4141 atctaatcat tccagatggg aaaacattag ctatcgtagg tccttccggt ggcggtaaaa
     4201 ccacgctttt acgtatcttg gctggcttag aaagttctga cagcggagaa ttttatttag
     4261 acggtgtacc gttcaaccca ttcgagctta aaatgaaaga acaagttgta ggagtcgtat
     4321 tccaagactt ccagctattt ccacatctga atatttttga aaacattacc ttagcaccaa
     4381 aaatggtatt gaaacagaca aaagaagaat acaccaaaaa agcagaagaa ttagcggctt
     4441 cattagggat tagtgattta ttgaatatct atccttatca gctttctggc ggacaaaaac
     4501 agcgggtagc tattgccaga gcattggcta tgaatccacg ggtattggct tatgatgaac
     4561 caacaagtgc tttagatcct gaattacgcc agcaagtcga gcgtttgatc ctatcattga
     4621 aagaagatgg ggtaacccaa attattgtca cccatgattt agtatttgca gaaaatgtcg
     4681 gtgatcaatt actaaaagtt tccccaattc attaagaaag gagaagatag agatgaaaaa
     4741 aaccagtttt ctattcgctt tgatcgcagg tcttcttctt tttgcaggct gttcgaacaa
     4801 aaaaacttca gcagataatt gggcgactat caacgaagag aaaaaaatcg ttataggttt
     4861 agacgataca tttgttccta tgggatttag agataaagac gggaagctca caggatttga
     4921 tattgattta gccaaagcag tctttgaaca atatgggatt acggtagact ttcagcccat
     4981 tgactggtca atgaaggaat ttgaactgaa caatggaacg atcgatttga tatggaacgg
     5041 ctattccaaa acgccggcaa gagaaaagaa agtgcaattt acaaaacctt atatggaaaa
     5101 cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca aactttggac aaatgaataa
     5161 taagagatta ggtgcgcaaa atgggtcatc tggttatgat gtatttacga aacaaccaga
     5221 agtattaaaa gagatagtca aaaaccaaga tgctgttttg tatgatagtt ttaatgaagc
     5281 attgatcgat ctgaaaagca gacgggtcga tggtctgctg atggataaag tatacgcaga
     5341 ctattatttg aaacaaagaa acgaactaga agacttcaac ataacaaaag gttcttatga
     5401 gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt acattagtga aaaaaatcaa
     5461 tgaagcccta gatcaactag agaaaacagg tgaattccaa aaaatatccg ataaatggtt
     5521 tggcgaagat gttacgccga gaaattaaca aataggggac tgtgacaaaa gacgtgtcac
     5581 agtccctttt tccgaataaa cagtgttctc ctgccaactc cttgtgactc ggagctgaat
     5641 ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc agtttttcgg tattaagcca
     5701 aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac tcgaaactaa
     5761 gcgacttttt cacaacctcc tttacataat tttattttgt tgtggaaggg gtttcgtgtt
     5821 attctaaaag aggaacgatt gaaggttata ggaaaggaag agatgaatgg aacacaaaga
     5881 acttacctca tttccaaaag attttctgtg gggctctgct tctgcagcgt atcaagtaga
     5941 aggagcttgg caagaagatg ggaaaggaga atcagtctgg gatcgatttg tacggatacc
     6001 tggaaagaca tttaaaggaa cgaacggtga tttggcagta gaccactatc atcgttataa
     6061 agaagatatt gctttaatga aagaacaagg tctgaaagct taccgcttct ctgtagcttg
     6121 gacacggatt tttcctaatg gacgaggaga agtcaatcaa gctgggctcg ctttttatga
     6181 gagattgatc gacgaattga ttgaaaacga tattgaacct atgttgactt tatatcattg
     6241 ggatcttcca caagcattgc aagatgaata taatggttgg gaatcacgtc agatcattga
     6301 agatttcact cattatgcag aaacattatt tgaggcattc cgcgggaaag tccattactg
     6361 gattagtttg aatgaacaaa atatcttcac ttcattaggt tatttgttag cagcacatcc
     6421 gccaggtgtg acagacccga aacgaatgta tgaagttaac catattgcta atttagcaaa
     6481 tgcatctgtc atcaataaat ttcacgagtt agaaatgcct ggaaaaatcg gaccaagctt
     6541 tgcgtatacg ccaaattatc caattgacag taatcctgaa aatgttctgg ctgctgaaaa
     6601 tgcagaagac ctgatggcca attattggat ggatgtctat atgtggggaa aatatccgat
     6661 tgctgccatg aggttcttag aagaaaaagg atgggcacca acgattgaag ctggcgatgc
     6721 agaattgcta gaatctgcta aaccagattt tcttgggatc aattactatc aaacggcgac
     6781 gaatgcattc aatccattaa acggagttgg tgctggaaaa atgaatacga caggaaaaaa
     6841 aggttcatca gaagaaactg gagttcctgg aatgtataaa aaagttgaga atccatttgt
     6901 cgaacgaaca aattgggact gggagattga cccagaagga ttaaggatcg gactgcgtcg
     6961 tatcacaagc cgttaccgca tgccggtact gattacggaa aatggcttag gagagtatga
     7021 caagttgaca gaagataaac aaatccatga cgactatcga atcaattact tacaaagcca
     7081 cgtcaaagcc atcaaagaag caatttcgga tggagcagaa gtcctaggct attgcacctg
     7141 gtcttataca gatttgctta gctggctgaa cggttatcaa aagcgatatg gatttgtata
     7201 tgttgatcaa gacgaaacac aagaaggatc tttggaacga tataagaaag acagttatta
     7261 ctggtaccaa aaactgattg aagaaaatgg agagaacctt tagttcacat acttaaaaca
     7321 agaaggtacg gctggatatg gccgtgcctt ttcttgataa aatcatttag aaattaagaa
     7381 aaactgtaaa ataagctgaa aaatgaaaga aagcgtcagt tctttcaaac aaaactttag
     7441 ttcccttact tttttttgta taatgaaact tgatgaaatg agggataata aatggcacaa
     7501 aagaaacggc ctactaaaaa aaggaaaaag acgaaaaaaa cgaaaaagca aaaacagcag
     7561 caggaacaaa tgctgacgat tggtgttggg tttgccttga ttttatttgc tgtcttcggc
     7621 tttttaaaat taggtttttt aggaatattg attgcaaacg gctttcggat cattgcagga
     7681 aatacttacc aaatcctctg tcttttatta gccgtattag gattttggat tgtcattaag
     7741 aatacagagt tttcaattgg taaaaaccga cgctggtttg gcggcatact cttttatttc
     7801 ggtatcttat tattgttaca tgcccattta tttggcaaac tccatactgg ggaaccaaat
     7861 atcatgggga cgacatggga tttgttagcc agtgatataa aacaaagtca agtagacaat
     7921 aacgttggtg gtgggatgat tggagcaatc ctttatcatt ttacttactt cttgattgca
     7981 cagcctggaa gttatttagc agcagtgcta ttactggcag gaggagcatt cctgttcagt
     8041 aatctggaag gctatcagct gttaaatggt atccaatcaa ttggagaacg agtacaggaa
     8101 ttattggaag gtgatcctgc caagcaagct cgaaagcaag cggcaaagga agagagaatg
     8161 aaacagcgcg cagaagcgaa agaagcaagg cgcttagctg caaaagaagc tgcagaaaaa
     8221 gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa tactgctgat
     8281 gaatggcagg aaccagaaaa tacagagccg gagcagctaa gttttgttcc aatcgacagt
     8341 tttcaagaaa atatccatcc tgcgaatcta gagaaaccag taccagatac accaaaacag
     8401 accaatacag cagaaggttt ttcagatgag ttgccagagg atgacggtac ctcattagaa
     8461 tttgagattg aagcagaaca ggaaaatcaa gactatgaat taccgacagt tgatttgctt
     8521 gattctatac caactgtgga tcaaagtgat gagtataaaa aaattgaaaa aaatatcggc
     8581 gtattagaac aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa agccagctta
     8641 ggaccatccg tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt aagtaaaatt
     8701 gtgaacttaa cagatgatat cgcgcttgct ttagcggcga aagatgtccg gatggaagca
     8761 ccgattccag gtaaatcact gattggtatt gaagtaccaa atggaaagat cagtatggtt
     8821 tcctttagag aaatcatcga agcacaacca aaccacccag ataaattact agaagtacct
     8881 ttaggacgtg atgtttctgg acgggtacaa acggctgatc tttcgaagat gcctcactta
     8941 ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa ttaatgggat tatcacaagt
     9001 atattaatgc gtgctaagcc ccatgaagta aaactgatga tgatcgatcc gaaaatggtt
     9061 gaattgaata tgtacaacgg catacctcat ctgttgacgc cggtcgtgac gaaccctaga
     9121 aaagcagcgc aagccttaca aaaagtagta caagaaatgg aagaacgata tgaaaaattc
     9181 gcggcaaccg gagtaagaaa tatttctggt tacaatgaat ttgttcaaca gaaaaacttg
     9241 gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga tgaattagct
     9301 gatttgatga tggtagccag caatgaagtt gaagatgcga ttattcgttt agcgcaaatg
     9361 gctcgggcag cggggattca tatgatccta gctactcaac gtccaagtgt ggatgttatc
     9421 acaggaatca tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc aagcggtaca
     9481 gattctcgga cgatcattga ttctaatggg gcagaaaagc tcttagggcg aggagatatg
     9541 ctattcttgc caatgggtga gaataaaccg atccgtgtgc aaggagcatt catttctgac
     9601 cacgaagtag aacgggtcgt tcaatttgtt acagatcagc aagaagctca ttatgaagag
     9661 aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc acaagatgag
     9721 ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc
     9781 caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga actggaagct
     9841 catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat tgagcaggaa
     9901 gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga tgaagggttg
     9961 aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa aatggttttt
    10021 ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga gattaaagtt
    10081 ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata gtatgtagcg
    10141 aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg gcttgacgaa
    10201 tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt
    10261 ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga atctatcgaa
    10321 acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac agatggtcgt
    10381 ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata aaaacatgat
    10441 tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg
    10501 atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac
    10561 tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg agcgcaatct
    10621 tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg ataattacga
    10681 caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg
    10741 gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg ctaataacgg
    10801 cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc gaacatcgat
    10861 catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg atgagtattt
    10921 agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag taaatgatag
    10981 accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat tagcatatgt
    11041 caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc
    11101 tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa
    11161 agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa
    11221 aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt
    11281 tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa
    11341 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
    11401 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
    11461 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
    11521 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
    11581 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
    11641 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
    11701 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
    11761 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
    11821 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
    11881 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
    11941 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
    12001 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
    12061 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
    12121 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
    12181 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
    12241 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
    12301 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
    12361 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
    12421 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
    12481 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
    12541 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
    12601 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
    12661 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
    12721 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
    12781 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
    12841 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
    12901 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta
    12961 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
    13021 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
    13081 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
    13141 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
    13201 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
    13261 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
    13321 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
    13381 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
    13441 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
    13501 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
    13561 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
    13621 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
    13681 aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta
    13741 cttttggaac accgtgtacc agaggttgtg aaaaagtcgc ttagtttcga gtagtaagaa
    13801 aaattttgtg aaaatggctt tctacatttt tgcataaatt tggcttaata ccgagaaact
    13861 ggcttttgaa caccgtttat tcagataagg gactgtgaca agacttttgt cacagtttct
    13921 ttttgtttat tcttttttat ctttgtatgg caataataga caatataacg aaaattattt
    13981 aatggctttt ggaagaccat ggaaaacctt gtacaaagct attttttctg aaaaattcct
    14041 tgaaacctca cgaaaaaatc attattttat gaaaaagaca aaaaaaagtg ttgatttttt
    14101 aata
//
LOCUS       DAZKOK010000076        13807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000076 DAZKOK010000000
VERSION     DAZKOK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.076"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG90W_001920"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG90W_001920"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0478268.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG90W_001921"
     CDS             complement(2260..2511)
                     /locus_tag="ACG90W_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0478269.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG90W_001922"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG90W_001922"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0478270.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG90W_001923"
     CDS             3461..3679
                     /locus_tag="ACG90W_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478271.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACG90W_001924"
     CDS             complement(3968..4480)
                     /locus_tag="ACG90W_001924"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0478272.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG90W_001925"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG90W_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0478273.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACG90W_001926"
     CDS             complement(6933..7376)
                     /locus_tag="ACG90W_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0478274.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACG90W_001927"
     CDS             complement(7377..8165)
                     /locus_tag="ACG90W_001927"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0478275.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG90W_001928"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG90W_001928"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0478276.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACG90W_001929"
     CDS             complement(9280..9867)
                     /locus_tag="ACG90W_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0478277.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG90W_001930"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG90W_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0478278.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACG90W_001931"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACG90W_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0478279.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACG90W_001932"
     CDS             complement(12784..13719)
                     /locus_tag="ACG90W_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0478280.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DAZKOK010000077        13780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000077 DAZKOK010000000
VERSION     DAZKOK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.077"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACG90W_001933"
     CDS             112..306
                     /locus_tag="ACG90W_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478281.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ACG90W_001934"
     CDS             575..874
                     /locus_tag="ACG90W_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478282.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ACG90W_001935"
     CDS             complement(971..1405)
                     /locus_tag="ACG90W_001935"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478283.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ACG90W_001936"
     CDS             complement(1637..2347)
                     /locus_tag="ACG90W_001936"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0478284.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ACG90W_001937"
     CDS             complement(2337..3197)
                     /locus_tag="ACG90W_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0478285.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ACG90W_001938"
     CDS             complement(3202..3840)
                     /locus_tag="ACG90W_001938"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0478286.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ACG90W_001939"
     CDS             complement(3855..4154)
                     /locus_tag="ACG90W_001939"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0478287.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ACG90W_001940"
     CDS             complement(4197..5681)
                     /locus_tag="ACG90W_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0478288.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ACG90W_001941"
     CDS             complement(5701..6162)
                     /locus_tag="ACG90W_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0478289.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACG90W_001942"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACG90W_001942"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0478290.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ACG90W_001943"
     CDS             7465..8235
                     /locus_tag="ACG90W_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0478291.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ACG90W_001944"
     CDS             complement(8508..9335)
                     /locus_tag="ACG90W_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478292.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="ACG90W_001945"
     CDS             9903..11138
                     /locus_tag="ACG90W_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0478293.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="ACG90W_001946"
     CDS             complement(12137..12397)
                     /locus_tag="ACG90W_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0478294.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="ACG90W_001947"
     CDS             complement(12424..13725)
                     /locus_tag="ACG90W_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0478295.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4428 a         2827 c         2172 g         4353 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga ccccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag
//
LOCUS       DAZKOK010000078        13724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000078 DAZKOK010000000
VERSION     DAZKOK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.078"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACG90W_001948"
     CDS             complement(45..1364)
                     /locus_tag="ACG90W_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0478296.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACG90W_001949"
     CDS             complement(1361..2014)
                     /locus_tag="ACG90W_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0478297.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="ACG90W_001950"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="ACG90W_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2820..3539)
                     /locus_tag="ACG90W_001951"
                     /pseudo
     CDS             complement(2820..3539)
                     /locus_tag="ACG90W_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3564..3749)
                     /locus_tag="ACG90W_001952"
     CDS             complement(3564..3749)
                     /locus_tag="ACG90W_001952"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0478298.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="ACG90W_001953"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="ACG90W_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="ACG90W_001954"
     CDS             complement(5356..6387)
                     /locus_tag="ACG90W_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478299.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="ACG90W_001955"
     CDS             complement(6394..7224)
                     /locus_tag="ACG90W_001955"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478300.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="ACG90W_001956"
     CDS             complement(7221..8036)
                     /locus_tag="ACG90W_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478301.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="ACG90W_001957"
     CDS             complement(8033..8857)
                     /locus_tag="ACG90W_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0478302.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="ACG90W_001958"
     CDS             complement(8847..9947)
                     /locus_tag="ACG90W_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0478303.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="ACG90W_001959"
     CDS             10125..10910
                     /locus_tag="ACG90W_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0478304.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="ACG90W_001960"
     CDS             10943..11326
                     /locus_tag="ACG90W_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0478305.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="ACG90W_001961"
     CDS             11402..11533
                     /locus_tag="ACG90W_001961"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0478306.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="ACG90W_001962"
     CDS             11502..11738
                     /locus_tag="ACG90W_001962"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0478307.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11750..11836
                     /locus_tag="ACG90W_001963"
                     /pseudo
     CDS             11750..11836
                     /locus_tag="ACG90W_001963"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11816..12787)
                     /locus_tag="ACG90W_001964"
     CDS             complement(11816..12787)
                     /locus_tag="ACG90W_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0478308.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13439..>13724
                     /locus_tag="ACG90W_001965"
     CDS             13439..>13724
                     /locus_tag="ACG90W_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0478309.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         4163 a         2983 c         2379 g         4199 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tact
//
LOCUS       DAZKOK010000079        13694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000079 DAZKOK010000000
VERSION     DAZKOK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.079"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..312
                     /gene="yaaA"
                     /locus_tag="ACG90W_001966"
     CDS             70..312
                     /gene="yaaA"
                     /locus_tag="ACG90W_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0478310.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            299..1423
                     /gene="recF"
                     /locus_tag="ACG90W_001967"
     CDS             299..1423
                     /gene="recF"
                     /locus_tag="ACG90W_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0478311.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1420..3366
                     /gene="gyrB"
                     /locus_tag="ACG90W_001968"
     CDS             1420..3366
                     /gene="gyrB"
                     /locus_tag="ACG90W_001968"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0478312.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3386..5857
                     /gene="gyrA"
                     /locus_tag="ACG90W_001969"
     CDS             3386..5857
                     /gene="gyrA"
                     /locus_tag="ACG90W_001969"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0478313.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6093..6392
                     /gene="rpsF"
                     /locus_tag="ACG90W_001970"
     CDS             6093..6392
                     /gene="rpsF"
                     /locus_tag="ACG90W_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0478314.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6442..6954
                     /gene="ssb"
                     /locus_tag="ACG90W_001971"
     CDS             6442..6954
                     /gene="ssb"
                     /locus_tag="ACG90W_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0478315.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6980..7216
                     /gene="rpsR"
                     /locus_tag="ACG90W_001972"
     CDS             6980..7216
                     /gene="rpsR"
                     /locus_tag="ACG90W_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0478316.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7359..9332
                     /locus_tag="ACG90W_001973"
     CDS             7359..9332
                     /locus_tag="ACG90W_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0478317.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9338..9790
                     /gene="rplI"
                     /locus_tag="ACG90W_001974"
     CDS             9338..9790
                     /gene="rplI"
                     /locus_tag="ACG90W_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0478318.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10064..11431
                     /gene="dnaB"
                     /locus_tag="ACG90W_001975"
     CDS             10064..11431
                     /gene="dnaB"
                     /locus_tag="ACG90W_001975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0478319.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12211..13503
                     /locus_tag="ACG90W_001976"
     CDS             12211..13503
                     /locus_tag="ACG90W_001976"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0478320.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4563 a         2477 c         3014 g         3640 t
ORIGIN      
        1 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
       61 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
      121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
      181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
      241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
      301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
      361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
      421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
      481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
      541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
      601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
      661 ttctttagta aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca
      721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
      781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
      841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
      901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
      961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
     1021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
     1081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
     1141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
     1201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
     1261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
     1321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
     1381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
     1441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
     1501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
     1561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
     1621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
     1681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
     1741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
     1801 tcttctgttg taaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
     1861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
     1921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
     1981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
     2041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
     2101 tatgacggcg ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
     2161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
     2221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
     2281 ggtggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
     2341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
     2401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
     2461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
     2521 atgaaattct tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta
     2581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
     2641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
     2701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
     2761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
     2821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
     2881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
     2941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
     3001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
     3061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
     3121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
     3181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
     3241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
     3301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
     3361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
     3421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
     3481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
     3541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
     3601 agatgtcatg ggtaaatacc atcctcatgg agacattgcg atctatgagt caatggtacg
     3661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
     3721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
     3781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
     3841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
     3901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
     3961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
     4021 attacctgga ccagattttc caacaggtgg tttggttatg gggaaatcag gaatccgaag
     4081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
     4141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
     4201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
     4261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
     4321 cgcatccgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
     4381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaatccttga
     4441 aaactatatc gaacatcaac gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa
     4501 agcagaagca agagcacaca tcttagaagg tttgcgtatt gctttggatc atatcgatga
     4561 aatcattgcg attatccgcg ggtctaaatc agatgacgag gcaaaagcaa caatgatcga
     4621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
     4681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
     4741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
     4801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
     4861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
     4921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
     4981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
     5041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
     5101 cgaaattcca gaatacgggc gtacagccaa aggaatccca atcatcaatt tactaggtat
     5161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
     5221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
     5281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
     5341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
     5401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg tcagagggat
     5461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt
     5521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
     5581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
     5641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
     5701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
     5761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
     5821 agaacaaaca gttgaagaac aaggatcaga agaataacca tcaagactaa gtgtgtaagc
     5881 tagcaagaat aaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
     5941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
     6001 taattagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
     6061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
     6121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
     6181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
     6241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
     6301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
     6361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
     6421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
     6481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
     6541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
     6601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
     6661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
     6721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
     6781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
     6841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
     6901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
     6961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
     7021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
     7081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
     7141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
     7201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
     7261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
     7321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
     7381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
     7441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
     7501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
     7561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
     7621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
     7681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
     7741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
     7801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
     7861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
     7921 tagatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
     7981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
     8041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
     8101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
     8161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
     8221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
     8281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
     8341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
     8401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
     8461 cttacatcgt tatcaatgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
     8521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
     8581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
     8641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
     8701 agtttccaag taaacctctg ctgacttata ttgaatcttc agcttcttca gcttcagagc
     8761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
     8821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
     8881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
     8941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
     9001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
     9061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
     9121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
     9181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
     9241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
     9301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
     9361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
     9421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
     9481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
     9541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
     9601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
     9661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
     9721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
     9781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
     9841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
     9901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
     9961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    10021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    10081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    10141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    10201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    10261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    10321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    10381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    10441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    10501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    10561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    10621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    10681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    10741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    10801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    10861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    10921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    10981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    11041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    11101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    11161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    11221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    11281 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    11341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    11401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    11461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    11521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    11581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    11641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    11701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    11761 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    11821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    11881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    11941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    12001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    12061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    12121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    12181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    12241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    12301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    12361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    12421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    12481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    12541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    12601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    12661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    12721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    12781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    12841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    12901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    12961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    13021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    13081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    13141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    13201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    13261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    13321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    13381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    13441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    13501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    13561 ctatacgata ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata
    13621 ggctgtctct tgaacactgt ttattcagaa taagggactg tgacaaaaac cgtgtcacag
    13681 tccctttttc cgaa
//
LOCUS       DAZKOK010000080        13324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000080 DAZKOK010000000
VERSION     DAZKOK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.080"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..144)
                     /locus_tag="ACG90W_001977"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="ACG90W_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="ACG90W_001978"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="ACG90W_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0478321.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1716..2498)
                     /locus_tag="ACG90W_001979"
     CDS             complement(1716..2498)
                     /locus_tag="ACG90W_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478322.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2563..3399)
                     /locus_tag="ACG90W_001980"
     CDS             complement(2563..3399)
                     /locus_tag="ACG90W_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0478323.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3417..4826)
                     /locus_tag="ACG90W_001981"
     CDS             complement(3417..4826)
                     /locus_tag="ACG90W_001981"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0478324.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4840..6663)
                     /locus_tag="ACG90W_001982"
     CDS             complement(4840..6663)
                     /locus_tag="ACG90W_001982"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0478325.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7506..7832)
                     /locus_tag="ACG90W_001983"
     CDS             complement(7506..7832)
                     /locus_tag="ACG90W_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478326.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7878..8240)
                     /locus_tag="ACG90W_001984"
     CDS             complement(7878..8240)
                     /locus_tag="ACG90W_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0478327.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8277..8491)
                     /locus_tag="ACG90W_001985"
                     /pseudo
     CDS             complement(8277..8491)
                     /locus_tag="ACG90W_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8619..8954
                     /locus_tag="ACG90W_001986"
     CDS             8619..8954
                     /locus_tag="ACG90W_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0478328.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9030..11027)
                     /locus_tag="ACG90W_001987"
     CDS             complement(9030..11027)
                     /locus_tag="ACG90W_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478329.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11125..11400)
                     /locus_tag="ACG90W_001988"
     CDS             complement(11125..11400)
                     /locus_tag="ACG90W_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478330.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11632..12366
                     /locus_tag="ACG90W_001989"
     CDS             11632..12366
                     /locus_tag="ACG90W_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0478331.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12405..>13324
                     /locus_tag="ACG90W_001990"
     CDS             12405..>13324
                     /locus_tag="ACG90W_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0478332.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDW"
BASE COUNT         4260 a         2634 c         2038 g         4392 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
     1501 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
     1561 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
     1621 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
     1681 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
     1741 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt tttcccattt
     1801 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
     1861 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
     1921 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
     1981 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
     2041 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
     2101 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
     2161 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
     2221 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
     2281 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
     2341 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
     2401 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
     2461 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
     2521 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
     2581 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
     2641 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
     2701 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
     2761 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
     2821 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
     2881 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
     2941 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
     3001 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
     3061 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
     3121 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
     3181 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
     3241 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
     3301 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
     3361 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
     3421 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
     3481 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
     3541 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
     3601 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
     3661 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
     3721 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
     3781 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
     3841 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
     3901 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
     3961 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
     4021 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
     4081 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
     4141 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
     4201 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
     4261 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
     4321 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
     4381 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
     4441 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
     4501 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
     4561 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
     4621 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
     4681 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
     4741 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
     4801 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
     4861 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
     4921 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
     4981 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
     5041 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
     5101 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
     5161 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
     5221 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
     5281 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
     5341 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
     5401 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
     5461 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
     5521 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
     5581 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
     5641 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
     5701 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
     5761 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
     5821 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
     5881 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
     5941 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
     6001 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
     6061 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
     6121 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
     6181 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
     6241 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
     6301 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
     6361 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
     6421 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
     6481 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
     6541 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
     6601 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
     6661 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
     6721 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
     6781 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
     6841 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
     6901 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
     6961 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
     7021 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
     7081 tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
     7141 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg atagaatgat
     7201 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
     7261 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
     7321 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
     7381 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
     7441 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
     7501 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
     7561 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
     7621 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
     7681 ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc taatccagat
     7741 gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat aactataatg
     7801 ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
     7861 tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
     7921 gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg atataagtag
     7981 cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc tactaccata
     8041 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
     8101 ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc agttgtctca
     8161 tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt tatttctttg
     8221 ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt tattagttaa
     8281 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
     8341 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
     8401 cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct ttttcttttc
     8461 tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt attatataca
     8521 acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca ttacaaatac
     8581 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
     8641 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
     8701 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
     8761 atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
     8821 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
     8881 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
     8941 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
     9001 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
     9061 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg aactaattca
     9121 actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt tgctaggtag
     9181 actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt agaagctcgt
     9241 tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc attttccacg
     9301 ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc tttctctgtc
     9361 attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
     9421 aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct tttagcctct
     9481 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaaat
     9541 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
     9601 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
     9661 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
     9721 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
     9781 ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
     9841 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
     9901 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
     9961 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
    10021 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
    10081 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
    10141 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
    10201 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
    10261 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
    10321 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
    10381 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
    10441 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
    10501 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
    10561 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
    10621 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
    10681 ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact atcatcttta
    10741 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
    10801 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
    10861 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
    10921 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
    10981 ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
    11041 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
    11101 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
    11161 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
    11221 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
    11281 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
    11341 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
    11401 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
    11461 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
    11521 tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt gtaaaattta
    11581 tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa tatgataaaa
    11641 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
    11701 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
    11761 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
    11821 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
    11881 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
    11941 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
    12001 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
    12061 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
    12121 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
    12181 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
    12241 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
    12301 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
    12361 atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa
    12421 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
    12481 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
    12541 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
    12601 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
    12661 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
    12721 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
    12781 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
    12841 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
    12901 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
    12961 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
    13021 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
    13081 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
    13141 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
    13201 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
    13261 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    13321 ggtt
//
LOCUS       DAZKOK010000081        13240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000081 DAZKOK010000000
VERSION     DAZKOK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.081"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..635)
                     /locus_tag="ACG90W_001991"
     CDS             complement(111..635)
                     /locus_tag="ACG90W_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0478333.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACG90W_001992"
     CDS             complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACG90W_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0478334.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACG90W_001993"
     CDS             complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACG90W_001993"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0478335.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2357..3037)
                     /locus_tag="ACG90W_001994"
     CDS             complement(2357..3037)
                     /locus_tag="ACG90W_001994"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0478336.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3163..4032
                     /locus_tag="ACG90W_001995"
     CDS             3163..4032
                     /locus_tag="ACG90W_001995"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0478337.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4058..4573
                     /locus_tag="ACG90W_001996"
     CDS             4058..4573
                     /locus_tag="ACG90W_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0478338.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4620..5183)
                     /locus_tag="ACG90W_001997"
     CDS             complement(4620..5183)
                     /locus_tag="ACG90W_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478339.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5238..5417)
                     /locus_tag="ACG90W_001998"
     CDS             complement(5238..5417)
                     /locus_tag="ACG90W_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478340.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5494..6003)
                     /locus_tag="ACG90W_001999"
     CDS             complement(5494..6003)
                     /locus_tag="ACG90W_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478341.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6099..6479)
                     /locus_tag="ACG90W_002000"
     CDS             complement(6099..6479)
                     /locus_tag="ACG90W_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0478342.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6809..7558
                     /gene="pnuC"
                     /locus_tag="ACG90W_002001"
     CDS             6809..7558
                     /gene="pnuC"
                     /locus_tag="ACG90W_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0478343.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7879..9096)
                     /locus_tag="ACG90W_002002"
     CDS             complement(7879..9096)
                     /locus_tag="ACG90W_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0478344.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9354..10631)
                     /locus_tag="ACG90W_002003"
     CDS             complement(9354..10631)
                     /locus_tag="ACG90W_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478345.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10710..11354)
                     /gene="pcp"
                     /locus_tag="ACG90W_002004"
     CDS             complement(10710..11354)
                     /gene="pcp"
                     /locus_tag="ACG90W_002004"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0478346.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11553..12497)
                     /locus_tag="ACG90W_002005"
     CDS             complement(11553..12497)
                     /locus_tag="ACG90W_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0478347.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12499..13185)
                     /locus_tag="ACG90W_002006"
     CDS             complement(12499..13185)
                     /locus_tag="ACG90W_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0478348.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3758 a         2770 c         2393 g         4319 t
ORIGIN      
        1 accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt
       61 tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt ctattcaatc
      121 aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc ccctctaggc
      181 atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct ttcattacga
      241 acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca actgatgatt
      301 tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat actgataccg
      361 acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc tgttccaata
      421 atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta tatataatcc
      481 aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa aactaaaaat
      541 tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc tcctggcact
      601 gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt tttctagaat
      661 tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat accgcttttc
      721 taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca gtgcttcttc
      781 taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt catctaaccc
      841 taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg gaatccgacc
      901 ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg ttatccctaa
      961 tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac cagtcaatat
     1021 cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt ctgtcattgt
     1081 taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt ttgccagtat
     1141 atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc agctgctgtt
     1201 tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt gataatcgat
     1261 aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg tttgttcaag
     1321 ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat tttatagccg
     1381 atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc tacttctgaa
     1441 tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg caattcgccg
     1501 tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac tactttgtct
     1561 acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac ttttggttcg
     1621 atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa ttcttgcgca
     1681 gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc tcttccacgt
     1741 accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat gatctgaagt
     1801 gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc gtctgcaatc
     1861 cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt
     1921 tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc atcccactta
     1981 tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct ggcatgcgct
     2041 gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc ttctgctttc
     2101 aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac gattttgatc
     2161 ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg acctccagac
     2221 gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat tttatctgta
     2281 tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg cactataaca
     2341 ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt tcttctccta
     2401 acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag ccacgacttg
     2461 cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta tcgatcattt
     2521 tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt tcccgttcat
     2581 ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga tgagaatacg
     2641 cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt ttagcccatg
     2701 acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc tctttataga
     2761 tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat aagccatgag
     2821 cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact tcttcataag
     2881 gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt tgggacgcat
     2941 attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat tctgaggaca
     3001 aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc taataaaaag
     3061 aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac ttgattaata
     3121 tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa attaattgca
     3181 tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga aaatgccgag
     3241 gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg tcgaaactat
     3301 caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat cactttgaac
     3361 ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat tccaaataat
     3421 caagcaatct ctgttttaga tattctagat gctaacggta tttactatga agtagcaacc
     3481 aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc ttccatggtg
     3541 gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc agctaagctg
     3601 gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga aaagaatctg
     3661 gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg tccagttgga
     3721 aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa caatatcgaa
     3781 gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg
     3841 atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag catgctgcaa
     3901 gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa
     3961 tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg cgcgattgca
     4021 gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt tcattcagga
     4081 aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt
     4141 tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg ccatgaatgg
     4201 cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt ttgagctgta
     4261 taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg gtgactttta
     4321 ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc aatatcaaat
     4381 ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt caggagatta
     4441 ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta ttgctgcggt
     4501 tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt cttaccgact
     4561 taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt
     4621 tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact
     4681 ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga
     4741 ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa
     4801 ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg
     4861 ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag
     4921 ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact
     4981 cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt
     5041 ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa
     5101 aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct
     5161 tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa
     5221 aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca
     5281 tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta
     5341 tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg
     5401 catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct
     5461 tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc
     5521 ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt
     5581 ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg
     5641 cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt
     5701 gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca
     5761 aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa
     5821 atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt
     5881 caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag
     5941 tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt
     6001 cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa
     6061 tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc
     6121 aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc
     6181 ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact
     6241 ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta
     6301 gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct
     6361 cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca
     6421 cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata
     6481 tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa
     6541 tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac
     6601 agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga aagtaagaaa
     6661 ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta taattgaaag
     6721 actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca gaacacacta
     6781 catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt tagctcatca
     6841 gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg gctgccaggt
     6901 catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta tcggtaccac
     6961 gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg
     7021 tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact atttaagtat
     7081 cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa gtaaagaatg
     7141 gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt ttatcgcaat
     7201 catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt atacagatga
     7261 tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg gtatcatcag
     7321 tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg ctcaaatggt
     7381 cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc
     7441 tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata accaaaaaga
     7501 acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac acgcataaaa
     7561 aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     7621 cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     7681 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctctattt
     7741 cctacgatta gtcaagtaaa aggtgcaaga aaaatagctc gtaaacgaaa gcgacaaaat
     7801 caaagcacac caaataaagc cccgttcgta aaaaattttc gatctgggca aagaagtggt
     7861 atactgtaag tggtttagtt aatggtccac tgagaccgcg tacgcatagt cgtactctgt
     7921 atgtgcccta accatagcgt gcatacattt cagaagcttg ttcatacaag caactgtggc
     7981 aaccttatct ttcttaggga taggttgctt ttttaatttg tagtaataat cgacaatatg
     8041 attaggagct gcgcgttgct ggcgaatcat atttcgaatg gtgaagaaaa taattttccg
     8101 tgctttggga ttaccacgct tattgatgcg gtcttggcca gtatacttac ctgaatgata
     8161 ccgtcggatg tcgattccga caaaggcatt caattgttta tgggttttaa atcgtgtgat
     8221 atctcctaat tcaccgagca ataaagcggc tgtgagtgcg ccaatcccag ggaacgtttg
     8281 gtacaacaaa aagcaaggag agccttcggc ttttttgatc atttgtgtcg ccagtgcttt
     8341 tttttgttcg agtaaatctt ggagaagttc ggcgtaatag atcgtttttt gacagtggat
     8401 actgtctttt tcaacagctg gataggcaca ctgtgcatag tggatgattt gatctgcctt
     8461 ttgctcggca cgattcgcgg agattttttt ccgtgtttcg ttgatcagtt tattttttat
     8521 ttttgtccga gtagatgcca atacaaaatc tggatgggga aaaagacgga tcaatgtcaa
     8581 tgcgtaaggg gtcagcctgt ttgaaaagaa ctgttcaagc tctggaaaag tcaattgaag
     8641 gcagttgtgt aaatccatgc gcagacgagt gattttcttc tcaatttctt gataaaaacg
     8701 ggctaaatcg cgcatctcta agtaaaaatt gctttgtact tcttttggct gacgttgatt
     8761 tttaagatga gtctgtgcga gccggtgggc atccgattta tctgttttcc aactgcgtaa
     8821 ggtgctttct tccatctgtt ttttcgcctc taacgggttt aacaaacagt aagaaaagtg
     8881 atgatcttga aagaactttt cgactggtct ggaataaatc cccgttgctt caaaaacaat
     8941 caatggacgc tcaggcaagt gctggatctc ttcgagtaat gcatgaaatc ctctttgagt
     9001 gtgttcaatc tcgtcttcaa ataagcaggt gtcgtctttg tataggacac tgtagctgtg
     9061 tcccattgaa acatctaatg caagaatgta aggcatttgt tttcctcctt tttaagtttt
     9121 gaaagaactt tactgcttca tttcgattcc aatttcctat acacgggctc atggccccaa
     9181 catacgtaaa cctgattcta aaatgacagt aaagaagttc ggtttttgat agggactcgt
     9241 agtcctcaag gctttgttcg aactttcttt cactcttcac tataatctaa aaatagaaaa
     9301 tagaggagaa catcattcgt caatgatgtt cccctctaat cttagtatgt tttttatagt
     9361 aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc taacttctga
     9421 taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc tttccatttg
     9481 tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg ccatttttct
     9541 tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc tttacgcaat
     9601 acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc ttctgtaaaa
     9661 ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat gaatcgttgg
     9721 tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg ttttgtcttt
     9781 ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac ttcttcatat
     9841 acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa ttcttctaca
     9901 tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg tcgctttttt
     9961 gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc ttgataatcc
    10021 gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc taattttata
    10081 tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca gtatttttgc
    10141 catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc gtctaaggcc
    10201 agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga gacatgcagg
    10261 tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg cgatttaacg
    10321 atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg gcttcgtttt
    10381 gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc agctaaataa
    10441 tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac tagttcccgc
    10501 tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta ttgtttagaa
    10561 attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa gacggattgc
    10621 cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac taacataacg
    10681 ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt tccggaaata
    10741 tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc aatgtcttct
    10801 aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga gaatggcacg
    10861 tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag caaacgatac
    10921 atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg tagaccatga
    10981 tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc ggctggaccg
    11041 ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat tcttgcttct
    11101 gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc ttgtccgaca
    11161 cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg actgcttttt
    11221 tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc atctggcatt
    11281 ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc aaaacctgtt
    11341 actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt tatcttttat
    11401 cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata acataacaga
    11461 tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag aagaggttgc
    11521 gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat ataagcaatt
    11581 tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc gtatttgtct
    11641 ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg tgtcagcagg
    11701 gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt cgctccatga
    11761 ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc aaatgcattc
    11821 cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc gatgttcaca
    11881 tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac tccagcgctt
    11941 gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg ccctactgtg
    12001 cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg agaaaagaac
    12061 atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc acctaaattg
    12121 gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat aaaaattttc
    12181 attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg gctttctcct
    12241 ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc accgttcacg
    12301 attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca gaaaaaggct
    12361 gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt gtaaatcaat
    12421 acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa aatatcagga
    12481 tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg ctaatttctt
    12541 ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca ctggaacttc
    12601 cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg ttgattggac
    12661 tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc agacattttc
    12721 catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt ctaacccttt
    12781 tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc cagcaactcc
    12841 accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa tatctacaat
    12901 cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt ctttcagacc
    12961 atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca tcccgcgatt
    13021 gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac cgccagtcaa
    13081 agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa atgcaaaccc
    13141 gagaataaca atcaagacgc ctaaaaggct aagataattt tccatatatt ttcctccata
    13201 aaatgacaat atttttttaa ttataaagca aaacacataa
//
LOCUS       DAZKOK010000082        13207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000082 DAZKOK010000000
VERSION     DAZKOK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.082"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG90W_002007"
     CDS             147..1016
                     /locus_tag="ACG90W_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0478349.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG90W_002008"
     CDS             1225..2250
                     /locus_tag="ACG90W_002008"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0478350.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG90W_002009"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG90W_002009"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0478351.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG90W_002010"
     CDS             3333..5036
                     /locus_tag="ACG90W_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0478352.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG90W_002011"
     CDS             5038..7071
                     /locus_tag="ACG90W_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0478353.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG90W_002012"
     CDS             7082..7522
                     /locus_tag="ACG90W_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0478354.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG90W_002013"
     CDS             complement(7722..8849)
                     /locus_tag="ACG90W_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478355.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG90W_002014"
     CDS             complement(8852..9529)
                     /locus_tag="ACG90W_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0478356.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG90W_002015"
     CDS             9793..11559
                     /locus_tag="ACG90W_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0478357.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG90W_002016"
     CDS             11574..12011
                     /locus_tag="ACG90W_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0478358.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG90W_002017"
     CDS             12024..13175
                     /locus_tag="ACG90W_002017"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0478359.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3694 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaa
//
LOCUS       DAZKOK010000083        13151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000083 DAZKOK010000000
VERSION     DAZKOK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.083"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..1857
                     /gene="yfcC"
                     /locus_tag="ACG90W_002018"
     CDS             118..1857
                     /gene="yfcC"
                     /locus_tag="ACG90W_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0478360.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2014..3198
                     /gene="iadA"
                     /locus_tag="ACG90W_002019"
     CDS             2014..3198
                     /gene="iadA"
                     /locus_tag="ACG90W_002019"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0478361.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3309..3512
                     /locus_tag="ACG90W_002020"
     CDS             3309..3512
                     /locus_tag="ACG90W_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0478362.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3613..4275)
                     /locus_tag="ACG90W_002021"
     CDS             complement(3613..4275)
                     /locus_tag="ACG90W_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478363.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4288..5790)
                     /locus_tag="ACG90W_002022"
     CDS             complement(4288..5790)
                     /locus_tag="ACG90W_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478364.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6338..8017
                     /locus_tag="ACG90W_002023"
     CDS             6338..8017
                     /locus_tag="ACG90W_002023"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0478365.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8144..8623
                     /locus_tag="ACG90W_002024"
     CDS             8144..8623
                     /locus_tag="ACG90W_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0478366.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8842..9522
                     /locus_tag="ACG90W_002025"
     CDS             8842..9522
                     /locus_tag="ACG90W_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0478367.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9530..10861
                     /locus_tag="ACG90W_002026"
     CDS             9530..10861
                     /locus_tag="ACG90W_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0478368.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10876..11838
                     /locus_tag="ACG90W_002027"
     CDS             10876..11838
                     /locus_tag="ACG90W_002027"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0478369.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11949..12287
                     /locus_tag="ACG90W_002028"
     CDS             11949..12287
                     /locus_tag="ACG90W_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0478370.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12366..13073
                     /locus_tag="ACG90W_002029"
     CDS             12366..13073
                     /locus_tag="ACG90W_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0478371.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4179 a         2321 c         2883 g         3768 t
ORIGIN      
        1 aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg tataattcat
       61 ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg
      121 aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat
      181 acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca
      241 gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact
      301 cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg
      361 tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg
      421 gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta
      481 gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat
      541 acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt
      601 ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta
      661 gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga
      721 gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct
      781 ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag
      841 gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc
      901 gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc
      961 ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca
     1021 ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat
     1081 gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc
     1141 cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg
     1201 atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc
     1261 tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt
     1321 gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct
     1381 ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt
     1441 gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc
     1501 gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa
     1561 agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc
     1621 atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag
     1681 cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta
     1741 ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc
     1801 ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct gcagtagtac
     1861 tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat
     1921 tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat
     1981 catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg
     2041 tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct
     2101 gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag
     2161 ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga
     2221 gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt
     2281 gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg
     2341 atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg
     2401 taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat
     2461 aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt
     2521 gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct
     2581 gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc
     2641 gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca
     2701 gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct
     2761 agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg
     2821 aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa
     2881 ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc
     2941 aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc
     3001 gccttacgag ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa
     3061 atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat
     3121 acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact
     3181 ttcgaaaaaa gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt
     3241 ttgtctgtat ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag
     3301 gaagcacaat ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg
     3361 aaggaaaaga agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag
     3421 cacaatcact aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag
     3481 acaagttttc tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc
     3541 aaataaaaaa tccgaaccat atctgcgaga cagatatggt tcggattttt tatttgatca
     3601 ttatcctgtt ttttaaactt cgattttttg aaatttttta ttttttagtt caaagacttc
     3661 atcacagctt tttgctactt caatctcatg agtcacgatg ataatacagc gattttgttc
     3721 atgggcgatt tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat ctagatttcc
     3781 agtaggctca tccgctacga ttagttgatg ttcacagcag atagcacgaa cgatagcgac
     3841 tcgctgctgc tgacctcccg aaagtttggt cacctgtttt tttgctaata tttcatcaat
     3901 tcctacacga gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt
     3961 gatttccatt gctgctatga cgttgtctag agccgataaa taaggaagca agttgtaagc
     4021 ttggaaaata atcgagacat catttttacg ataacttctt aacccgattt tgttcagtga
     4081 acgatcttga tagtatacct ttccttcttt tggtgcatct agtccagaaa taagcgaaag
     4141 aaaagtcgtt tttccagaac cacttgctcc taaaatagcg tacatttttc cagcttcgaa
     4201 attcagttct acatctttat aaagcgcatc ttgcggatct tgataccaaa atcccaattg
     4261 ttctgtccgt aacatcaaat tcctccttta gtttaatagg atttttcttg ggtttaatct
     4321 taatatccct atagaagcta aaaagacaga aaacagactg attccaaatg ctactccggc
     4381 taatagtccc aattgtgcag gtttgacatt gatttccaat tcgctgactt gatcagaaac
     4441 agcaaatgga ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt gttcttgtct
     4501 gttacctggc atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg
     4561 ttggtcaagt agctgctgtc ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac
     4621 tcctaatgca agaatcatac aaatagccac ttcggtaaac aattggagaa tgattttact
     4681 tctggattct cctaaagata gcaagacgcc taactcatgc cgacgctccc gaatggtaat
     4741 cataatgatc agagtaagta tgataatacc ggctacagcc actagtaaaa caacattttt
     4801 agaaaaactt gccacgttgt ttaaaggttc caacattgac tgatacatgg aatcatttga
     4861 ttgtaagcta aatgtatctg tgtcgatcag cttctcagct tctttgagaa actcatccat
     4921 tttatctgga tcagatagag tgtaaatagc agaatctatc gtgttatcgt cagatgtccc
     4981 atttagttca tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt
     5041 gaatttcata cccatactgc cgcttgtttc tctggattca taaattcctt tgatcgtcat
     5101 ttcataagtg gtatcttcat cttcagtact cgtgattgtg aaagtatctc cgactgccag
     5161 gtcatttgtt tctgctagag tagaatcaat gactacattg tttgtgcctt tgtcgctgtc
     5221 atctattcct tcaccatccg tgatttttgc tgttccatca gaaaaattac tattttgtgc
     5281 agttgctgac acaccactga tttgaaagtc tgcttgagtc atttgcggat tcccgcccat
     5341 tcctccagca ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga
     5401 actagtgatg gcagttattc catcttttgc catagctgag gcggaacttt caaaggaata
     5461 actctttaca ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag
     5521 agaaaaactg cctggatctg gacgttcttc agtggtagaa ccgcttgaag aagtttgttc
     5581 ctgtttttta aatgaagctt ctcgattggc acttaaagtc actgttgctc caacttcttt
     5641 ttgtgcttga ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa
     5701 aataagaatg gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt ttacttttgt
     5761 acttttccat gcacgtttca taaaattcat attttgcatc cctcctagta gatgcatttg
     5821 ataatagaca acctatctta aagctagctt aaatcttatg ctgtttgcac accatcaaca
     5881 tgaggttctg tatatataaa aagtcactat agttattgca aaaaatgtgg taaaatgaaa
     5941 aaagtatgtt tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag aaatcctaga
     6001 tgctacagag ggatttttac gtgcatagtt gttctaacaa ttgctgatac tagcgattta
     6061 gtttatttgt tgtataataa tggggaacag ctgaatactt atataagtat tctggctata
     6121 gcgtttcgca aaagatccat tttgttaatg aagtatatcc aaaataaaat gcccatccaa
     6181 ctgtcagaac gcggatgtgc tggtaatttt cctgctgatt ctccataagt tgagaaatag
     6241 tgattttgca tatgcttcat tggaatggct catatattta gataaagaag aaaatcgaca
     6301 tctttaaact tggatgtcat aagaagaggt gaagtttgtg agtaccatta aaattatccc
     6361 attaggcggc gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat
     6421 ttttatttta gattgtgggc taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt
     6481 aatccctgat tttacgtatt tagaagaaaa cattgatcgt gtagctggtg tctttttaac
     6541 acatggacat gcagatgcga tcggggcact tccttacttg ttgtcaaaaa tttctgtacc
     6601 agtattcggg acagagttga cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc
     6661 agccaaagga tttaaggatt tccatgtggt agatgagcat acagaaatag actttggtga
     6721 aacagtagtg agctttttcc gtaccactca tacgatacca gattcagtgg ggatcaatct
     6781 gaaaacaaaa gaaggtagta tcgtttatac gggagatttt aaatttgacc aaacggctat
     6841 cccaatgtat caaacagatt atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc
     6901 cttactaagt gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg aagcccaaat
     6961 agcagatgaa gtatttgata cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt
     7021 agcaagtaat ttgcaacgag tgcaacaagt attggatgca gcagaaaaag cacaacgaaa
     7081 agttgtcttg actggtcaag atttcgaacg aattattcgc acagcgatgc gtttagaaaa
     7141 acttcaatta ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa actatgatcc
     7201 agaagaactg atcattttag aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa
     7261 aatggcaaac ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg tttatatcac
     7321 tactacgcca agcatcgcga tggaaaccat cgtggcgaaa acggaagata tcatttatcg
     7381 agctggtgga acagtgaagt tgatctctga gaatatgcgc gtttctggcc atgccaatcc
     7441 taatgacttg caattaatga tcaatttgat gaaaccaaca tattttgtgc cagttcaagg
     7501 agagtatcgt gaattagctg ctcatgcaga tttggctcat gcagtaggga tgccatacaa
     7561 aaatatctat attactggac gcggagacgt gctggaatat aagaacaagc gtatgagtgt
     7621 tgcaggaact acaagtgcag aaaatgtaat gattgatgga ttaggcatag gtgatatcgg
     7681 aaacattgtt ttacgtgatc gaaaaatctt gtcagaagac gggattttcg tagcggttgt
     7741 cactatcagt cggcgagaaa agaaaatcat ttcaaaacct caaattactt cacggggatt
     7801 cgtttacgta aaagctagcc gtgatctgat gaaagaaagc agcaatatga ttgaaacaat
     7861 cgttgagaaa catttagaaa gcaatgattt tgagtggagc aaattaaagc aagacatccg
     7921 agatcaatta ggccgttact tatttgaaca aacaaaacgc cgcccagtga tcttgccagt
     7981 cattatggaa gctactcaac gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc
     8041 tctatagagt gctcgtcttg ctcttttttt gcgaaaaaca aataattttt gaatacagca
     8101 tccctcgctt tataataaac gatagaataa agggagtgaa tcaatgatga gtcaaacaaa
     8161 agtcgaacgt tatttagaag aacaaaagat cagctatcaa ccacttgatt ttagtcatgt
     8221 ggtagaagaa tctttcacag aagaactaca aaaaagaggg attgatccag ctttgatatg
     8281 taaaactctc gtagtgaaag gaaacaaaac tggggtgatc atcgcattga ttcctttgaa
     8341 cgatcactta gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc
     8401 tggaatggat tttgtattaa tgcatacagg atatccccac ggagcaaaca cccctattgg
     8461 aataaaactc gcacatcctg attattgttt tttagcagac agttctttaa aagaaaaaga
     8521 agaagtaatt gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag tagccgattt
     8581 agaaaagatc gtccatccta tttacgctga tttaattagt taagggacga gaagagaagc
     8641 taagcagagc agagacctca aacttgagat tatatctgct tggtacttgg tgcatcctgt
     8701 attatctgtt acaattgctt tgtacatact gtactatcaa tccaatgaaa atatatttag
     8761 cagctatatt ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag
     8821 agaaaacgcg aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg tttaacaatt
     8881 gaaatggaaa atggccaacg cgtgatgatt gacccgttta ttacaggaaa tccattgaca
     8941 gacttaactg cagaagaagt aaagacagat tggttgctga ttactcatgg tcatagtgac
     9001 cacataggag atatgattcc tattgcaaaa gaaaatcaag ccacaattat cagtattgtt
     9061 gagattgctg attatgcgaa cagcagagga gtagattcat tcggtatgaa tatcggtgga
     9121 aaacatgcgt ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat
     9181 gaagtagacg gtatcgttca gtatatgggc gagcctagcg gaattattat tcaagcagaa
     9241 ggaaaaacga tttatcatgc aggggatact gcttatttca gtgatctagg attgcttgca
     9301 gaagaattcg atattgatgt cgctttcttg ccgattggcg ataactatac gatggggcca
     9361 gaagatgcag caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc aatccattac
     9421 aatactttcc ctgtcattga acaagatccc gaatcttttg tagagttact ggaagatagt
     9481 gaaggaaaaa taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa
     9541 acatgatttg attttagaat atattgaaag tttacctgtt ggcaatcgaa tttctgtaag
     9601 aagcatcgca aaagaattga atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc
     9661 agaaaatgtc ggactagttt caacaattca acgagtaggg accatccgta tcgaacgcaa
     9721 attaaaaaaa catattgaac gtttgacatt tggcgaggtc gtcaggatca ttgaagggga
     9781 cgttttaggc ggggcggcag gattagacaa ggtcttgaat aaattcgtca tcggcgcgat
     9841 gactaaacat gccatggaac gctatattac gccaggatca ctaatgatcg tcggcaaccg
     9901 ggcggaagtc caaaaattag cattggaaga tggcgccgca gtattgatca caggaggatt
     9961 caatacgact gctgagattg caaagctagc tgatgatctt gaaatgccga tcttacgcac
    10021 ctcttatgat acatttacag tcgcaaccat gatcaaccga gcgttaagcg accaattgat
    10081 caaaaaagat atcatgcttg tcagtgatat ctatatgcca gctgaaaaga ctcattattt
    10141 gcagcagaca gatacgattt ctgattatca gcgtttgtca gaagaaacgc agcattcgcg
    10201 ttttccagtc gtcaaccgcc atcatcgttt gatggggatt gttacggcaa aagatgtttt
    10261 agggaaatcg ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa
    10321 gaaaacaatg agtattgcct ctgttagcca tcaaatgatc tgggatgggc tagaagtcat
    10381 gccggtcgtt tccgatgact tgacattgga aggcttgatt acccggcaag atgtaatgaa
    10441 agcgatgcag cttgtccagc gacaaccgca gattgcagat acgatttcgg atcagatatc
    10501 tggaaatatc catacgattg atactgaccg tgaaggaaat cgtttggaaa accctaagtt
    10561 caaattcgta gtagcaccac aaatggtcaa tggcgttgga accatttctt ttggcgtact
    10621 tagcgaaatc atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat
    10681 cttgattgaa caagtgaacc tacattattt gcggctgatc caattggaaa gcgaattaga
    10741 cattcgtcca cgtgtattgg aaatcggcag acgttccgct aaactggata tcgaagtgtt
    10801 tattgaaaac accatcgttt ccaaagcaat cgttgtttgt caagtaatgg aacgttctta
    10861 gaaagcagga gtgaaatgac agtacagcaa gaaatagtag atacaataaa aaaatacgat
    10921 aaaatcatta tccacaggca tttgcgccct gatcctgatg cattaggatc acaagtagga
    10981 ttagccgaac tattacggga aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg
    11041 attgaagggc ttcgcttttt agcagaaatg caagaagttg aagatgtctt ctaccaagga
    11101 gcgctagtca tcgtgactga tacagctaat tcacccagaa tcagtgatca aagatatcag
    11161 ttggcagaaa aactgatcaa aatcgaccat catcccaatg atgaccctta tggagatctt
    11221 gtgtgggtaa atacaaaagc aagtagctgt agtgagatga ttgcagaatt tgctttgatg
    11281 tttcctgaat ctttaaagat gaacgttaag gctgcacggt tactttatgc aggaatcgtc
    11341 ggagatacag ggcgcttttt gtatccctcc acgacgtccc gtacattaga gattgcttcg
    11401 atattgagaa attatccatt tgatgcaagt gctttgaaca gagagattga acaaatgccg
    11461 atgaaagtcg ctaaactttc cggttatctt tatcaaaata tccaggtgga tgaaaatggt
    11521 gcaggaagaa taatccttcc gcaagaaatt cttgataaat atggaatcga tgattcagag
    11581 acagcttcga tcgtttcact tcctggagtg attgacgaag tgctggcttg ggggattttt
    11641 gttcagcagc cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat
    11701 gaattggcta aacgccatca tggaggaggc catccattag caagtggtgc gaatgcaaaa
    11761 gatttagcag aagtagagga aatctataaa gagatccaag atatatgcca atcgtataag
    11821 agcagaaaaa cagaatgata cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa
    11881 tgtatctttt caagatcttc tttttccgtt acaatagtgc tcactaaaga ttttaaagga
    11941 gaaagaagat gacattacca aattgcccag aatgtggctc agagtacaca tatgaagatc
    12001 gaggcttgtt gatttgccca gaatgcggga acgaatggaa caaagaagaa caagaaactg
    12061 tcatagaaga cggattagtg gtattagatg caaatggaaa ccgtctgtta gagggcgata
    12121 gcgtgacagt gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa
    12181 caaaagtcaa gaacatccgt ttagtagaag gcgatcataa tatcgattgc aaggtagaag
    12241 gatttggacc gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg
    12301 tcttttcaca ttcttttcct actactaatg ataaaataaa gtaaacgaaa aggagtgaaa
    12361 cgaaaatgta tggttatgga atgtatggtt ttttagatcc gacatttctt ttggtcattc
    12421 ttggattagt tatttccgga attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg
    12481 atcaagtgag aagcaaaaac aatgtgactg gtacacaagc agcacaatat atattacaaa
    12541 gtcaacagat aaataatgtc ggtgttcaac aaattgccgg agatctcaca gataactaca
    12601 attcaggtaa taagatgttg agtttgtctc aagcaactgc acagtctact tcagttgctg
    12661 cgataggtgt agcagctcat gaatgcggac atgcggtaca agatgcttcg ggttatgtcc
    12721 cattgaagat tcgtgcagca cttgttccag cagctaactt tggttcgatg atttcgttcc
    12781 ccttgattat ggtaggtgta ttgcttagct ggaactctac gttaatcaat atcgggattt
    12841 tcgctttttc tttagcatta cttttccaat tggtgacatt accagtcgaa tttaacgcgt
    12901 ctcggcgagc aatccaaatt ttgagtgaag gcggactact gacggaagaa gaagtaccga
    12961 tggcaagaca cgtattattt gcagcagcac tgacttatgt tgcagcagcg ctttcgacat
    13021 tcctgcagct gttgcgcttg atcttattgt tcggcggcaa ccgaagagat taaaataaag
    13081 aagttgtgaa atcagcgctt tgatctgagc taataccgag agttagctga ttgaatacca
    13141 tttatttgga a
//
LOCUS       DAZKOK010000084        12922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000084 DAZKOK010000000
VERSION     DAZKOK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.084"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(554..2167)
                     /locus_tag="ACG90W_002030"
     CDS             complement(554..2167)
                     /locus_tag="ACG90W_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0478372.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="ACG90W_002031"
     CDS             complement(2308..3216)
                     /locus_tag="ACG90W_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0478373.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3399..4523
                     /locus_tag="ACG90W_002032"
     CDS             3399..4523
                     /locus_tag="ACG90W_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0478374.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4548..4727
                     /locus_tag="ACG90W_002033"
     CDS             4548..4727
                     /locus_tag="ACG90W_002033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478375.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4750..5667
                     /locus_tag="ACG90W_002034"
     CDS             4750..5667
                     /locus_tag="ACG90W_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478376.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5660..6493
                     /locus_tag="ACG90W_002035"
     CDS             5660..6493
                     /locus_tag="ACG90W_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478377.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6576..7694
                     /locus_tag="ACG90W_002036"
     CDS             6576..7694
                     /locus_tag="ACG90W_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0478378.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7715..9586
                     /locus_tag="ACG90W_002037"
     CDS             7715..9586
                     /locus_tag="ACG90W_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0478379.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9580..10803
                     /locus_tag="ACG90W_002038"
     CDS             9580..10803
                     /locus_tag="ACG90W_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0478380.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10889..12823)
                     /locus_tag="ACG90W_002039"
     CDS             complement(10889..12823)
                     /locus_tag="ACG90W_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0478381.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
BASE COUNT         4183 a         2151 c         2501 g         4087 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     3421 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     3481 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     3541 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     3601 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     3661 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     3721 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     3781 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     3841 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     3901 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     3961 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     4021 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     4081 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     4141 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     4201 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     4261 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     4321 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcca
     4381 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     4441 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     4501 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     4561 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     4621 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     4681 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     4741 gacttaacga tgatgcaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     4801 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     4861 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     4921 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     4981 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     5041 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     5101 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     5161 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     5221 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     5281 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     5341 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     5401 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     5461 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     5521 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     5581 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     5641 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     5701 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     5761 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     5821 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     5881 gattgttatt gctgtaatca gtacagtttc agctacagcc agttctacaa tggcgggtta
     5941 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     6001 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     6061 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     6121 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     6181 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     6241 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     6301 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     6361 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     6421 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     6481 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     6541 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     6601 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     6661 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     6721 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     6781 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     6841 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     6901 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     6961 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     7021 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     7081 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     7141 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     7201 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     7261 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     7321 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     7381 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     7441 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     7501 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     7561 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     7621 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     7681 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     7741 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     7801 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     7861 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     7921 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     7981 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     8041 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     8101 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     8161 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     8221 atcagcactg aaagaagcat ttgataatat agaaaattgt tatcttcaaa atggctggta
     8281 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     8341 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     8401 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     8461 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     8521 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     8581 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     8641 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     8701 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     8761 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     8821 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     8881 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     8941 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     9001 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     9061 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     9121 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     9181 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     9241 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     9301 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     9361 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     9421 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     9481 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     9541 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     9601 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     9661 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     9721 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     9781 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     9841 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     9901 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     9961 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
    10021 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
    10081 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
    10141 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
    10201 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
    10261 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
    10321 caaggttata gtgataattc ctgttgggct agaggccaag catgggggat ttacggtttt
    10381 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
    10441 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
    10501 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
    10561 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
    10621 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
    10681 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
    10741 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
    10801 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
    10861 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
    10921 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
    10981 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
    11041 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
    11101 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
    11161 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
    11221 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
    11281 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
    11341 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
    11401 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
    11461 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
    11521 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
    11581 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
    11641 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
    11701 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
    11761 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
    11821 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
    11881 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
    11941 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
    12001 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
    12061 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
    12121 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
    12181 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    12241 aatggttcgt tggttcgtct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    12301 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    12361 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    12421 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    12481 tgtctggcgt accttcgcta atgaaaatct ctagctcgcg catccgctct tccatttgtc
    12541 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    12601 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    12661 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    12721 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    12781 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    12841 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    12901 ttatactgcg agatttgcag aa
//
LOCUS       DAZKOK010000085        12844 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000085 DAZKOK010000000
VERSION     DAZKOK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.085"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..644)
                     /locus_tag="ACG90W_002040"
     CDS             complement(<1..644)
                     /locus_tag="ACG90W_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0478382.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKL"
     gene            complement(654..1469)
                     /locus_tag="ACG90W_002041"
     CDS             complement(654..1469)
                     /locus_tag="ACG90W_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478383.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1731..2174)
                     /locus_tag="ACG90W_002042"
     CDS             complement(1731..2174)
                     /locus_tag="ACG90W_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0478384.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(2308..2646)
                     /locus_tag="ACG90W_002043"
     CDS             complement(2308..2646)
                     /locus_tag="ACG90W_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0478385.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2634..3011)
                     /locus_tag="ACG90W_002044"
     CDS             complement(2634..3011)
                     /locus_tag="ACG90W_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0478386.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            3235..4428
                     /locus_tag="ACG90W_002045"
     CDS             3235..4428
                     /locus_tag="ACG90W_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0478387.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4594..5016)
                     /locus_tag="ACG90W_002046"
     CDS             complement(4594..5016)
                     /locus_tag="ACG90W_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478388.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            5411..5605
                     /locus_tag="ACG90W_002047"
     CDS             5411..5605
                     /locus_tag="ACG90W_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478389.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5602..6096
                     /locus_tag="ACG90W_002048"
     CDS             5602..6096
                     /locus_tag="ACG90W_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478390.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(6258..7430)
                     /locus_tag="ACG90W_002049"
     CDS             complement(6258..7430)
                     /locus_tag="ACG90W_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0478391.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7636..8451)
                     /locus_tag="ACG90W_002050"
     CDS             complement(7636..8451)
                     /locus_tag="ACG90W_002050"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0478392.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8601..9047)
                     /locus_tag="ACG90W_002051"
     CDS             complement(8601..9047)
                     /locus_tag="ACG90W_002051"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0478393.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(9044..9559)
                     /locus_tag="ACG90W_002052"
     CDS             complement(9044..9559)
                     /locus_tag="ACG90W_002052"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0478394.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(9632..10546)
                     /locus_tag="ACG90W_002053"
                     /pseudo
     CDS             complement(9632..10546)
                     /locus_tag="ACG90W_002053"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(10543..>12844)
                     /locus_tag="ACG90W_002054"
     CDS             complement(10543..>12844)
                     /locus_tag="ACG90W_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0478395.1"
                     /translation="KIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFTFLGSLSSIY
                     VNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQLIDPTEKEIT
                     AAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVAVNGSMSADK
                     TALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNTIDSVTPNAD
                     KSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKGNVSNFPTYQ
                     AATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVTGYSFAQTNG
                     SFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQDLTTNTPTIN
                     GYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKTNATVSINAN
                     GSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNEKGVEVTAPT
                     EIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGSKTYDGKAGV
                     PSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTNSGIDKVKAA
                     NPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVTGVVLGESLD
                     YTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPSTGGIGLLPF
                     VFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3690 a         2505 c         2335 g         4314 t
ORIGIN      
        1 atcaatttac gattgttgaa gtaaatcaaa ccaaatacga tagaaggacc tagtagaata
       61 caccattggg gctttacgaa aaagaagaga aacaaacttg tcaatagtaa aaaatctagt
      121 aaactcacat accatttttt caacgtatag tagaacgcta tccgtagaac ttgatttggt
      181 ttcgacgctg gatttctcac ctgaaaatac cagtagttga tgctaaagga aactgataaa
      241 caagcaagta aaacaaacaa cggaatcaac cattttccat aagaaaaacg tataaaaaag
      301 aaacaatcaa aaatcattat cacactaact atccaagcaa atagccaata aaaaaatccc
      361 tttagccaaa attggcgaaa cgtctttatg aacaaacgta ctggtgaaaa acttttttct
      421 tttgctaatt cttctgtcat acctatcatc acgatgcaac ctggaccaat aaaaagtaaa
      481 gaaaaaataa aggaaaagac attccttcca tctatcgaca aaaggaacgc agctgctata
      541 tacaagatat tgacacacca aaaacaaagg ctacttgcca atgattgata caaccaataa
      601 aagaattcca tataaacatt tttgtcaaaa cgctgctctt tcataatcac ctcctattcc
      661 ttcattcctg atgaagcgat cccttctaca aaatattttt gcatcgctaa aaatataaaa
      721 gcaacaggaa gaacagaaac aactgaggca gccatgatta atgcgtactc tgcatcaaat
      781 agcccaataa acattttcaa ccctaactgg attgttttat tttccgtaga agacaaataa
      841 atgaatggcc ccatataatc gttccatgta ttaacgaatg tgatgatcgt caaactggca
      901 atcgccggtt ttgtaagagg taaaatgatc ttgcgataga ttccccactc tgataagcca
      961 tcgatccgcg cactctcaca taaagaatct gggatactac tgtagtattg cttcaacaag
     1021 aacacaccaa aagcattgaa cgcttgtaat agcaccaacg accacaacgt atatgttaaa
     1081 cccacttttc tcatcataat gaactgcggg atcatataag actgccaagg gacagcaatc
     1141 gtgccgatat atgctaaaaa taaaatatcg cgccctttaa aatttagttt agaaaacccg
     1201 taagcagcaa aactagaagt aaacaactga atcaacgtga tcacaacgct taaaaaagct
     1261 gtattcttaa aaaatgtcac taaaggaata cgagaccaaa taaccagata attctctaaa
     1321 tgccaagttt tagggatcca ttgaatcgga atcgtgaaca cttcattatt tgctttgaat
     1381 gaagcagaaa ccatccataa aaaaggaatg atcgtcaata aagctaatac aatcagcaga
     1441 atcataccca accattgaat aaagggcatg ttttttgaac gaatttttgc tttttttctt
     1501 tcatttcgaa cacctccaaa gttataatct tcatttcttt gtaatgatta caatccagaa
     1561 caacggttat tatagctgat tcatttccgt tcttcttatg tttgtaagcg cttatttaac
     1621 aagttatgta aatctcttta ttttttttga tttaaaaatg aaaaaataca aaagcaagac
     1681 tgtcatacgt ctcgcttttg tattgaaatt tcttcgatta gagatactta ttactgttca
     1741 aaagcttctt tgaaacggtg aatcaattca ttgactacag gtgataaaat ccgattcttt
     1801 ttccagacta gcatgcagtt cgtttcaatc attgggtcaa gcggaacgaa tgtcactcgc
     1861 tttgaaagat gacttacagc accttctatg actaaagcag cacctacctg attctcaatt
     1921 agcgcaaata cattgaaaat caaattgtac gttcctgtga tcagcaactg ctcctttttt
     1981 atgcctaacc agtcagcaaa cattcgctgt atttctggac gctttgagat caaaagaggt
     2041 acattcgcaa agtcttcttt tgttatcacg tctttttttg ctaaaacaga attttctaaa
     2101 atgagcaatc cccatttttc ctttcttggt aacaaaatcg tttcgtattt ctctgtatct
     2161 actggatcta gcaaaatcgc taaatctaac aggctttttt ctagtcactc cgtgatatca
     2221 tcacttgttc cgctgtggat atgaaattga acgctgggat aatcacggat caacttttct
     2281 agtaatagtg ccaatgtatt cgaattatca gcttccacac agccgatcgt aaatcggccg
     2341 ctgaataatt gtttttttct gttctcgaat tcttgagtgg tttgattcgt taaatctaaa
     2401 atttcttctg ctcggctttt ttagaaaata acctgcttct gtcaagatca ttttgcggtt
     2461 ttcgcgtaca aacagttctg ttcctagttc ctcttctaac tccttcagtt gtcggcttaa
     2521 ggttggttgt gtaagatgta gaacttctgc ggcttgagag attgttcctt cttctgcaat
     2581 tgtccaaaaa tattttagta agcgtaactc catagcgatg ctccttgatt tttttacttg
     2641 atacattcca ttttatttct agataacaca cgatcatatt tcttgatttt tccttgtaaa
     2701 cgttccaaac actcttgcat ttcagcaatt tctttcgtca aaagttcact ttcttcaatc
     2761 aaaagcttac gccgctcaaa tacagttgct tctccttcgc tagataggcg aatatattca
     2821 atcaaagact cgatcgataa tccggcattt cgcatacaga tcgtaaagtc gacccatttt
     2881 aggtcttctt ctgtataatc acggattccg ccttttgtcc gattaactgg tggaatcaac
     2941 ccgattcgtt cataatagcg caatgtatcg gccgaaatgc ccttttcttc actgacttgt
     3001 ttgatattca tctgatcact ctttctctta atttcttgcc actttactta tgtattttat
     3061 ttattctaca actttaagtg tactccaagt caatactcat ttctattaaa actaagctgg
     3121 attatctata tcggttgttt gtgtattatt tcaaataaaa aagtcttgtt tttttattct
     3181 tttttggata tacttttaac aatttaggga aattttagga aaaggggtat aaaaatgaca
     3241 acgtatcagc gaaaggtatt attatcgaca tcggcgggaa ttgctttaga gaatatggat
     3301 attatgtttt tagctttttc attatcttca atgattactt cttttcatat tagtggtacg
     3361 caagcgggtt tcatttcaac gatcactaat ttggggatgc tcattggagg tatttttttt
     3421 ggcatcatag ctgataaata tggacgtgtg aaagtatttt ctcaaacggt ccttctgttt
     3481 tcgattgctt ctttattgat gtattttgct tcaaacatct acttggttta tctctttcgc
     3541 tttatcgcag gtgtaggagc gggaggagaa tacggggcgt gtatgtcctt gatttccgaa
     3601 agtttttcaa aaaaacaaat tggacgagct tcttcggttg ctggtattgg tgcgcaggtc
     3661 ggtgcggctt tggctgcgat tttagctgct gtgatcattc caacgtttgg ttggaagatg
     3721 ttgtatgtcg taggtgtact gccggtgttg atggtcttgt tcattcgtcg cggcttgact
     3781 gaaccaaagg aattcaaatc tgtacaatct gccgaaaaga aaaccaaact tagcagactg
     3841 tttgaatcaa aacatcttgc ttggcaaaca tgtggcctta gtatcatggt aactgtccaa
     3901 attgccggct attttgggtt aatgaattgg ctgccttcaa ttatgcaaaa tcgattaggg
     3961 ctttcggttt ctgggtcttc attgtggatg gtcagtacta ttttaggcat gtctttgggg
     4021 atgatgactt ttggcataat catggataaa ttggggccta aactagcctt ttctttgttt
     4081 ttaatttgtt catccttatc tgtctatcta cttgtgttag cccatagcca gtggagtttg
     4141 gtagcagccg ctgtgatagt cggctacttt atcaatggca tgtatggtgg atatggcgca
     4201 gtgattagta gtttgtatcc aacagaaatc cgcgcgacgg cgaataattt cattatgaac
     4261 cttggacgag ctgttggtgg tttttcttct atcgtcatcg gtttcttgat ggatcattat
     4321 agtttaactg ccgtaatctt gtttttgagc ggtatttacc tagtcagtct gctggtcttg
     4381 acaactctta ctggtgttac agcattaagg gaaagcttgc agcattgata gttgagtctg
     4441 taaatgacaa gaaagaggtg tggcaaaagc ttgcatctgc ggtaataaga ccagccaaat
     4501 caaaaacatg agacactgtt tccgtttggc tggtcgtatt acttgatgca gggcagcttt
     4561 ttcaactgct aatttccttc tatctggcgg cgactattct ttgacgtccg tcatttcaaa
     4621 gtactttact aataacttag cataacgaaa aactgtggat acactcgtat attgagcgtc
     4681 tgcataatct tccaaatttt tctcaggatg ttgatacatg aattcaatca aatgataatt
     4741 atatgatttt ctgtagaaat gaagataaat aatgtagatt gggaagttct cctgcttttc
     4801 catagaaaag cgccgattat tctcattctt caaaatgacc ttatcaggat atttttggaa
     4861 caattcttct aaatctgtta gataattcaa gatagactta cttgatacct tcaattcgtt
     4921 ttgtagttct tctattgaac accaacgttc tgtttcaagc agtaaatgaa tcattttaat
     4981 tttcttttga gaaacttttg ttaaaaaagt ctgcatattt tctcctcctg tatctacttt
     5041 actatactac atacaagaaa aaaaacggat acaaatatca tattgtcaca ccgtcaatag
     5101 tgcataaacc tgttattatt tctctttttt tcctaagtat tctattgata tgaatcattt
     5161 aatcgtttat tatataaatg tcaataatct aataataaaa taacgtacat attttattat
     5221 tgcatactta ataacagaac tatagcaaaa aatcccgtgt atcaacacgg gattagatta
     5281 agtttcctca atcactattc ataacacaat tattataaca agtaagtaaa caattgaaaa
     5341 ggacatttat tgaaaacggt tgttttttac ttaaggagga aatttagcga aaaaattagt
     5401 tgcattgttt ttggtcgcag gaagtttgtt gagtgggtgt ggtgctacta cgtctactac
     5461 tcaaagcaca agttcagatc aggcttccag cagcagtacc acgatctccg aaagtacggc
     5521 agattcatcg aaagaacaag aacaaattga aaagaaaaaa gaagagaaac aaaaagcgga
     5581 aaaagctgca ctagaaaaaa agtgacagaa gcggaatctg ctttgtcaca agctgaagca
     5641 aatcctacaa aagaaaacta tgcaactgct tcagcagcta tccaagcgct acctgaaaac
     5701 aaacaagagt tgacaagccg tcttgctgca attgattcca caatcaaagc aaaagaagca
     5761 gaagaagaac ggaaaaagca agaagaaatt gcacgacaac aagcagaaca agcgcaacaa
     5821 gaagcaatcg cacagcaaca agcagaacag gcgaggcaag aagcaatcgc acagcaacaa
     5881 gcagaacagg cgcgacagca atcagcagct gaacaggcct ctcagcaggc acaagccgca
     5941 gctcctgagc aaaataatga acaaactgtc tatgtcacac cgaccggttc aaaataccat
     6001 acacataaat gtggaaatgg tacgtatacc cctgccactt taagcgaagc tcaagctcgt
     6061 ggtttaaccc cttgctccaa atgttatgga aactaattca aaagttatac tataataata
     6121 aaattcttct gaaacataaa aaaatagaag atggcgttcc agatagtaaa ccaaaaataa
     6181 cagtttacac gtccatttcg tcagcttctc tcttttttca tttagtctgt tccctctgct
     6241 tttttggaca tcttcattca ttctttctca atcaaatagt acacctcttt ttcttttcgc
     6301 agtttatatc ttcctttttt caatttcatt gaaaactgtt tcgaatgttt atgttttaca
     6361 tggattttaa gcaggagctc ctcatgttgc aagcgatagt tcctgccttt taaatcagaa
     6421 aatctcacta gtccgtattc atcagaagct ttcacaaatg gaactttttc tctagtaata
     6481 tagtgtttgc cgttgagcgt ttttacagaa aagaccattc cttcgattgg tcgtccgttt
     6541 gtgttcttta cataaaaaga taaaggatag tttcgcttag cgatggccaa agaatctaaa
     6601 taagcgatga gtagccaaac taagccgatt gatccaagta gtataccgat aaaccaaata
     6661 aaaaatttcc attttttcca ttgatcaatt ccttggatcg ctttttttgc ctcttttgct
     6721 tgataaggaa tacgatgccc cgtcacaagc agtcgatgtg tattgaccat ataaggagta
     6781 caggtaagca aagtcacgag atctctgcct ttttggatac ccaaagaatc tacttcatca
     6841 ggtaagacca cttggatttt ttctacttga tacgccaatg tctttccatt gatctggatg
     6901 aaaaactggt ctcctttttt caacttaggc aaatctgtaa ataattttgc ttctgggagt
     6961 ccacgatgcc cagaaagaac ggcatgggtg tttttcccgc cagtcggata agaactttct
     7021 tccaataatg acgttccttt ttgcaaaaat atttccgttg tctgatcgaa tataggtaag
     7081 ctgacattga tttttgggat ttcgatgact ccaagcatat gttccataaa aaaggcatta
     7141 ctaggtaaat cttttaatgc ttttgcatca actgcttggt tgaaactgcc tatccctggt
     7201 gatacgtttt gctctgccaa ctgttggttt ttctttgtca ttttttcctg ctgttttttt
     7261 atttcctctg aattcttatt cgatgcttgc ttttgataat ggatgatcat ttgctgggca
     7321 agaaaatcat tcagtgaatc ttctacaaaa ggataagaaa agatcccgat cccaacgact
     7381 aaaatgattg ccatgaagat gtccagtatt cttattctgc ttttattcat catgtccacc
     7441 tattttctaa tatgtatatg aagaggttgt gaaatcagcg ccttgacctg agtattagcg
     7501 aagcaaacgg caaaatagtt cctcctattt tgccgtttgt tgagcgaata ccaagagacc
     7561 gaccacttgt cacaaccttt ctccccattt gaatcactat cgtttattta tcgtatgatt
     7621 tttgtttcca atagtttaag cttctgtttc ttgatggcgt tttttgaaat agatcactgc
     7681 tcctccaact aaagcagtac ctaccaagat gaatacgatg atccccatac caccagttga
     7741 aggcaagatt cctttaggtt ggttatcgat cgttgtttca tcagaagttg ttggatcatc
     7801 atctactgta aattcagtgt acgcagcttc attcttaggt aatgcgtagc cttctggtgc
     7861 ttttgtttct tgaaggcagt atgccccttg tttcagccct tcgattgaaa atttcccttc
     7921 tgaatcagaa atcaatttca cttcttccca gtcagaacct gtgacttcat ctgaccaagc
     7981 atatgggtat tctcctgagg caaggtttgc tatttttttc attaatttaa cagttggatc
     8041 ttttgctctt agaatagaaa attctgcatc ggctaatttt tcttgcgtgt tcacatcttg
     8101 tttgatgaag tggaaatcac cgtagttttg gacattttct acagtaatcg tgttatccgt
     8161 agttgtattt tcccgattga ctacaccgtt tggattccca agattggctt catctgcgcc
     8221 atggatgata ccggttgttt gttgcaattt tccgtttttt actataaaag tagtttcttt
     8281 ttgtggattg gttaattgat aaccatctgg tgcttttgtt tcaactaaag tataattgcc
     8341 gtctattaat ccattgacac tgatggatcc ttcagtatca gttgtgaatg tcaacgctga
     8401 ctcagcagga tcatagagag cgacaccagt gttttcatca aaaccggtca cgaattgatt
     8461 ctctgaattt ttgatctgga attgcgcccc tgctaactta tctacccctg aaaagttccc
     8521 agctttctgg attttgatat tgctttcttt gatgacattt ttagcatata ggtgaatcgt
     8581 gtctaaaggt ttgtcagggt ctagggtaag cttaacacga tatttggcga cgtttcgaca
     8641 ccgggctttg gtgtttctac aaagacgtat acagcatctt tcccatcaga tttacctgga
     8701 agagtcaggt tagctaatcc ttcaccagaa gtcgttacac ttgcgaaggg tgtagcgtct
     8761 gctggaactg ctgtgtcttt acttggatag tacgtagcag tgatgtcata tacgttgaat
     8821 tcaacgccgt tcaacggttc gctattcgga aatgtcaatt ccgttccgtt gtttatttga
     8881 ttttctggtg ctgaagtgaa tttcaattta tgcaaattaa ccgtaacatt ttctggtgta
     8941 ggtgatacgg ctgcagaaac ctctgcagtt cctaggccga gaacgaaagg aagcagcatg
     9001 atagcagttc ctaaaaacgt ccatattttt gttttgatgt ttttcatttt atttccctcc
     9061 tattgtttcc tttttgttgt tttttataaa accaagctgt ccctgttgaa aggactagca
     9121 ataaccctaa aattgagaaa cctccaagca tctcttcgct tgtttttgga aagcttcctc
     9181 catttttttc atttgatgga ccatttgtct gcatctgaac tggcgttttt ccattattcg
     9241 tcggatttgc ttttggttcg ttgatgattt ccaatggtat aggatgaagc ggatcataac
     9301 tgcctttttc aacttcaaag agaatggttg attgactcaa tatatatcct ttaggcggtt
     9361 taatctccac tagttcgtaa cttccaaaag acaatccatg gacagaaaat gttccatctt
     9421 tactggaagt aaaaatcgtt gctcttgaat catctttggc tacccaatga tttatcccgt
     9481 tttcttgcgc taagtattgg tcattttttt tgactagaaa ttgagcatct gctagttttt
     9541 gtttcgtttg ccgatccact ttgacaaatt gtttgcctcc tgtttggatg gcaagatgtg
     9601 tttctactgt ttgattgtca gtcgtcaaac gtacatcgtt atcaaatgtc gttttttcac
     9661 tgttttcttt caatgtcatt tggtaacgga tcgttaattt ttttccagca aacgaactaa
     9721 gatcttttcc agaatctgat aagacaaaac catgttttat tttttgtgtg tgaaatgtat
     9781 gaatcttgtc tccaccaatc aaaaaatcga tcgtttctgg ttctagccat aactgaggat
     9841 cagctgtatc tttgatcgta taggttccta tctcgttgat gttctcagga attgttacgg
     9901 tgatgagata agggacttta tccccatctg caaaatctgt ctgcttattc tctactgttt
     9961 ttgttaatgt gagtgcagca ggtatttttt gttcgctctt tggatacaag tggatggtcg
    10021 tcaatttatg attagatgaa tctgtcacag gaagcacaac gacaaatggt gctgattttt
    10081 cttttactta atctgaagtc tttatttccg taaatcggta aacagcgtca cgaccttgct
    10141 catctttgtc cgatgcacta aaagaagcaa tgccctcttc ctcgtctact gtttttgtca
    10201 cattttctgc cagtattttt tcactatcac ttttttgtgc tagttttctt tgtgcgtctt
    10261 ccacactact tccttctgac cgcagcttgt aaaattcatc cgagacatca tatatagcga
    10321 aagtaacgcc gttcaaccct tttactcctt gaaagttatc cgggatattt attccgtcgt
    10381 tttgactgat acttggcatc ttatcgcttt cgaacattcg attatgcaaa gtaaatgtaa
    10441 taggtgattc tctagctgac acagtcggag aacctataaa aaagaccagt aggataaaaa
    10501 agagaacagc aaatatatcc acacgtacat tttttgtttt tttcatcgct gttcacctgc
    10561 ttttttctta cgatggataa aataaaagaa gccagaaaaa ataaagatca gtcctaaaaa
    10621 gacaaagggc agtagaccta tcccacctgt cgaaggaagc aagagagtcg gattgatcgt
    10681 caacttagcg gtatctgttg cgataatcgt ataattattg tttacacttc cttcgcctaa
    10741 actaatcgtt actatttgtg atccaatatc tgtccggctg ttaactgtta gggtataatc
    10801 caatgattcg cctaagacaa caccggtaac gtggagattt ccaaccgaaa tcgagtgact
    10861 ttgggcatca taattaaagg cagcgtcatc gacagtaatc actgcgttag ctttatagat
    10921 ggtataagtt cctgatgtgt tggctatatc agtaaatgac aacttgggat ttgctgcttt
    10981 gactttgtct attccgctat tcgttagttt aattgtatag ctgccagcat taaccatatt
    11041 ttttgctgat tgtgcatctg ctgtgtaatc catactgcta agtgccactt gtactggagt
    11101 aagcgaacta gtgacaccct ctgctagctt gacatggatt gacgggacac ctgcttttcc
    11161 atcatatgtc ttactgccac tgagctctac cgcaccgctg atttgcttaa tcgtatagac
    11221 atcagatgtc tgcacgatag taaataaata gtttgttctg gcagctagtt tagctagtcc
    11281 gctttttgtc aattgccaag acgtataggt tccaatttca gtaggcgcag ttacttcaac
    11341 tcccttttca ttcatcgctt tgaaatcagt tggattccat gtgatttgat caatactgag
    11401 atcattgttt aaaagggatg ttgattctgt tttatcatta aaggcatatt taacgatcgg
    11461 tacaacagaa agaccatcat agtacttact gccatttgca ttgattgata ctgttgcatt
    11521 tgtcttataa tataggatga cttcagaaat ctgttttcca tttgcgtcct ttacgattac
    11581 atttccattt gtatctaaac tagctaaaac actcattgct gattggttct ttgtttctat
    11641 cgctgaaaca gctgtaaagg tatatccgtt gatagtaggc gtattagtcg tcaggtcttg
    11701 tgcagcactt ccgtaagtaa gattcattat tttagaagga gcaatcatct gtccaaaata
    11761 gtcttgatac ttaatagtga ttggtgtttg tttgcctgta taggtcaccg tatcgacgtt
    11821 actttctagg gctgtgaaag aaccgtttgt ttgtgcaaaa gaatagcccg ttactgctgt
    11881 tggagtgata accactttgt cacccacatg gattccagat aatgtagtcc cgtacgttcc
    11941 gatttttgct gctccaccat tgactgtata agaaaaagtt cctgtccgat tcgcattgac
    12001 ctgaaccgtt gctgcttgat aggttggaaa atttgaaaca tttcccttac catcaacgat
    12061 ctgcccattt ttgatcgagt accccgtgaa tggaatatca tttgctaact gtataccgat
    12121 tgaaaaagta ctcatacctc ctccggcagg aagccctccc catgttgagg gtgtaaaaga
    12181 ataactttta tccgcattgg gtgtaactga atcgatcgta tttccagcgg tagccacccc
    12241 catatagata ctttttggga atgtgatagt agtaccattt actgtggagc caataaacag
    12301 attatggata tctggacggt tatcaataaa agcgattgct ttggttacgc cttgtaacgc
    12361 agttttatcc gcactcatcg atccgttaac cgcaacaatg actccatctt tggcatatat
    12421 cactgtatat ccatctggta tggtatgtcc gttgaaattc gcagtggccg caacagtact
    12481 aaatgtctgg cttctacctg tttctgaata agctttctcc cctgctgctt ttgctgctgt
    12541 gatttctttt tctgtcggat caatcagttg agtcgaggat gtggttgagg ttgtctcgtt
    12601 gttgctagac tgattggtgc tttctatgga ctgtatttca gaatagggtg gactatttgt
    12661 ctcctctgtc gtttggttag caaaatttga cgtttctgta taaacgttta cataaatact
    12721 tgataagctc cctaagaaag taaaaatcaa gatgatcgaa atcataatgg gatgtaagta
    12781 ttttctttta gatacagctt tgagtctaga aacaaaatgt ttttgacata tacgttggat
    12841 ttta
//
LOCUS       DAZKOK010000086        12670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000086 DAZKOK010000000
VERSION     DAZKOK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.086"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG90W_002055"
     CDS             6..305
                     /locus_tag="ACG90W_002055"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0478396.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG90W_002056"
     CDS             378..1739
                     /locus_tag="ACG90W_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0478397.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG90W_002057"
     CDS             1761..2021
                     /locus_tag="ACG90W_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478398.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG90W_002058"
     CDS             2035..2787
                     /locus_tag="ACG90W_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0478399.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG90W_002059"
     CDS             complement(2881..3486)
                     /locus_tag="ACG90W_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0478400.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG90W_002060"
     CDS             complement(3539..4513)
                     /locus_tag="ACG90W_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0478401.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG90W_002061"
     CDS             complement(4891..5610)
                     /locus_tag="ACG90W_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478402.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG90W_002062"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG90W_002062"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0478403.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG90W_002063"
     CDS             7161..8558
                     /locus_tag="ACG90W_002063"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0478404.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG90W_002064"
     CDS             8639..9079
                     /locus_tag="ACG90W_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0478405.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG90W_002065"
     CDS             9227..9553
                     /locus_tag="ACG90W_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0478406.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG90W_002066"
     CDS             9554..10303
                     /locus_tag="ACG90W_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0478407.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG90W_002067"
     CDS             complement(10383..10838)
                     /locus_tag="ACG90W_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0478408.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG90W_002068"
     CDS             complement(11265..11540)
                     /locus_tag="ACG90W_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478409.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG90W_002069"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG90W_002069"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0478410.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4054 a         2175 c         2588 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DAZKOK010000087        12635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000087 DAZKOK010000000
VERSION     DAZKOK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.087"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACG90W_002070"
     CDS             complement(146..1111)
                     /locus_tag="ACG90W_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0478411.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACG90W_002071"
     CDS             1345..2190
                     /locus_tag="ACG90W_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0478412.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACG90W_002072"
     CDS             2381..3568
                     /locus_tag="ACG90W_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0478413.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACG90W_002073"
     CDS             3574..3930
                     /locus_tag="ACG90W_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0478414.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACG90W_002074"
     CDS             3932..4270
                     /locus_tag="ACG90W_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0478415.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACG90W_002075"
     CDS             4665..5699
                     /locus_tag="ACG90W_002075"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478416.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACG90W_002076"
     CDS             5692..6378
                     /locus_tag="ACG90W_002076"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0478417.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACG90W_002077"
     CDS             6402..7250
                     /locus_tag="ACG90W_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0478418.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACG90W_002078"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACG90W_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0478419.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACG90W_002079"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACG90W_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0478420.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACG90W_002080"
     CDS             9516..10751
                     /locus_tag="ACG90W_002080"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0478421.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACG90W_002081"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACG90W_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0478422.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACG90W_002082"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACG90W_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0478423.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4179 a         2366 c         2798 g         3292 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atata
//
LOCUS       DAZKOK010000088        12493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000088 DAZKOK010000000
VERSION     DAZKOK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.088"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG90W_002083"
     CDS             <1..375
                     /locus_tag="ACG90W_002083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478424.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG90W_002084"
     CDS             complement(324..893)
                     /locus_tag="ACG90W_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0478425.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG90W_002085"
     CDS             complement(967..2256)
                     /locus_tag="ACG90W_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478426.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG90W_002086"
     CDS             2514..3458
                     /locus_tag="ACG90W_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0478427.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG90W_002087"
     CDS             3472..4545
                     /locus_tag="ACG90W_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0478428.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG90W_002088"
     CDS             4535..6706
                     /locus_tag="ACG90W_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0478429.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG90W_002089"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG90W_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0478430.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG90W_002090"
     CDS             complement(9009..11195)
                     /locus_tag="ACG90W_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0478431.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG90W_002091"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG90W_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0478432.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG90W_002092"
     CDS             complement(11417..11857)
                     /locus_tag="ACG90W_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0478433.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG90W_002093"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG90W_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2631 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aac
//
LOCUS       DAZKOK010000089        12175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000089 DAZKOK010000000
VERSION     DAZKOK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.089"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..301)
                     /locus_tag="ACG90W_002094"
     CDS             complement(137..301)
                     /locus_tag="ACG90W_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478434.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            596..1924
                     /locus_tag="ACG90W_002095"
     CDS             596..1924
                     /locus_tag="ACG90W_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0478435.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2126..2959
                     /locus_tag="ACG90W_002096"
     CDS             2126..2959
                     /locus_tag="ACG90W_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0478436.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2975..3712
                     /locus_tag="ACG90W_002097"
     CDS             2975..3712
                     /locus_tag="ACG90W_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0478437.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3867..4835)
                     /locus_tag="ACG90W_002098"
     CDS             complement(3867..4835)
                     /locus_tag="ACG90W_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0478438.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5067..5900
                     /locus_tag="ACG90W_002099"
     CDS             5067..5900
                     /locus_tag="ACG90W_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0478439.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5934..6773
                     /locus_tag="ACG90W_002100"
     CDS             5934..6773
                     /locus_tag="ACG90W_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0478440.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6799..7359
                     /locus_tag="ACG90W_002101"
     CDS             6799..7359
                     /locus_tag="ACG90W_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0478441.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7558..9279
                     /locus_tag="ACG90W_002102"
     CDS             7558..9279
                     /locus_tag="ACG90W_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0478442.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9444..10586
                     /locus_tag="ACG90W_002103"
     CDS             9444..10586
                     /locus_tag="ACG90W_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0478443.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10602..11813
                     /gene="thiI"
                     /locus_tag="ACG90W_002104"
     CDS             10602..11813
                     /gene="thiI"
                     /locus_tag="ACG90W_002104"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0478444.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2527 g         3455 t
ORIGIN      
        1 aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt tcttttatta
       61 ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac gaaagagtag
      121 agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa taaatcagca
      181 ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc ccaaagttat
      241 ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc aggtttttca
      301 ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt attttgaaag
      361 ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc attttcgtta
      421 tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac atttttcttc
      481 atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga gattgtattt
      541 ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg attggatgaa
      601 agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa atatcaaaca
      661 gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg aaaatgtttc
      721 agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga aagtggctaa
      781 tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc gtcataagat
      841 gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt ttttctggga
      901 taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa aagaggcaat
      961 cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg gccaatggga
     1021 tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga tgccggaaag
     1081 tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac tgcgtaaaga
     1141 tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta ttgaagaaaa
     1201 gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag gaacacctcc
     1261 aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg accgaggact
     1321 gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt atgttagcgt
     1381 gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag agatgctagg
     1441 aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa ctttcaaaaa
     1501 attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg atgtaggtca
     1561 atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg atgatctatt
     1621 tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa gtctgatgac
     1681 ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa aatggaaagt
     1741 cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga gtgatgcctg
     1801 gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga aagagcaatt
     1861 aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg gtgctttagc
     1921 atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg cttaagccag
     1981 ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta taattctaaa
     2041 taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac gaagtagaat
     2101 aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg atctgaaatt
     2161 aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc gtataaaaac
     2221 cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat tttttgaagc
     2281 agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata cgtatatgct
     2341 aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg gaaaagggga
     2401 tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc cacaatttgg
     2461 ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg aagaagattg
     2521 gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag aagaaacgaa
     2581 tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg tattgaaatc
     2641 ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga gtggtaatgt
     2701 gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt atgggaatcg
     2761 ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat ttttagatgc
     2821 ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga tttaccagtt
     2881 atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc aagtagatca
     2941 attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata ccaaaaagca
     3001 tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt cgatgttagg
     3061 agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca gtgaattcgt
     3121 caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta atcgtctccc
     3181 tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata agtttttctg
     3241 ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa tagaatctgt
     3301 tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag agatcattgc
     3361 tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg aaaagattga
     3421 ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta atcagcatgt
     3481 taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact ttttgactac
     3541 gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg ggaatgagat
     3601 cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac atgaaatccg
     3661 aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat aaaaaagagg
     3721 ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat tgtttctcaa
     3781 atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag cctctctttt
     3841 taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa ttcttcaccc
     3901 gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact attaatactg
     3961 ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata atcataaaca
     4021 ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt ttggacaaga
     4081 agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa agcttcgatt
     4141 accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg catcccagca
     4201 tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg ctttgtttct
     4261 aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg acccaatgta
     4321 ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg tgcagaatgg
     4381 atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca acttgcgaca
     4441 cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact tgaacgcatt
     4501 gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa aaaagcagtt
     4561 tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga tgcataagcg
     4621 gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg tgagggaaga
     4681 ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc tgcatctaat
     4741 aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt catggcgatc
     4801 gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt acgaataatt
     4861 ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat cagtgaagaa
     4921 taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa aagactcaac
     4981 cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa agcagccaag
     5041 gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg tttaaagaac
     5101 atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg tttctaccct
     5161 tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt gagaattgga
     5221 taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga atcagcggtc
     5281 aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat tttttagatg
     5341 ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa ttcccctcat
     5401 cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag tggtttgagg
     5461 gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa tttcttgaac
     5521 gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat gagccacagt
     5581 tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt gctctttttc
     5641 gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg cgtaaaaaaa
     5701 gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa gtcaaagtag
     5761 ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt ggtgatgcgg
     5821 ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga ctcaatccaa
     5881 cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag tctatgatca
     5941 aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa gcgttggaat
     6001 caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt gtagcaacgg
     6061 ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat atgtatgtcg
     6121 tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac ccttttgaac
     6181 aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa atcgtttttg
     6241 gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg atttttatca
     6301 agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa aaaatcctcc
     6361 aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata ttaaaagaac
     6421 caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta gaacatcgct
     6481 ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc ccaaaaggtg
     6541 gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa atggaagaaa
     6601 ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta ggaattggag
     6661 tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg actcaatcaa
     6721 atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat tagtcaatga
     6781 aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga aacattccac
     6841 aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa tgaagctcat
     6901 acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac ttcacctaat
     6961 aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa agcagaagaa
     7021 aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt agatgcactg
     7081 acagatatcc tttactttac ttatggttct ttttcattac taggaatagt gcctgatcga
     7141 atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg aaaacctcat
     7201 tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga atatgctcca
     7261 gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc taagaagaaa
     7321 tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca ctatttttgt
     7381 tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg catgaaaata
     7441 gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt ggatttatgc
     7501 caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga accataaatg
     7561 ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat tgtcgtcttt
     7621 ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt agaaaaacgc
     7681 aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa aaaaatgcat
     7741 ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa tgacatatct
     7801 acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct caatgaaagt
     7861 tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat ggaccaaatg
     7921 gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac ggaagaacgc
     7981 aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga aaaagtcaga
     8041 gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat caaagtgatc
     8101 gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt tgaagcccgc
     8161 gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat gaaaaaaatt
     8221 ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga aatcagtgat
     8281 acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct tgctgaagat
     8341 atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa aacagaagtt
     8401 gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt atatgatatc
     8461 ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac gattgcagag
     8521 tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga tcacacatct
     8581 caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca aaccgaagtg
     8641 gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca cgaaattcct
     8701 tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga tatcgaaaag
     8761 caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa agaagcgcaa
     8821 gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt agaaaaacaa
     8881 cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac tgaccgtatt
     8941 gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt caatcgttta
     9001 gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga tttagtagat
     9061 gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca tacacacgaa
     9121 gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata tcactatcaa
     9181 gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc tttcaaacgc
     9241 atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt tagtaaacca
     9301 gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt gaaaattgaa
     9361 acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata atagaaaaga
     9421 atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca accccaatct
     9481 ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg aatccatcaa
     9541 gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga aagcagatcg
     9601 ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga acagaaggag
     9661 ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat catatgatca
     9721 tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa gaattaggaa
     9781 tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac gaattgaaag
     9841 aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat gaagtaggat
     9901 cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa attcatttcc
     9961 atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg acagaaagag
    10021 ttgattttgc aacattttca gctcataaat tccatggccc acggggaact ggtttcattt
    10081 attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa gaaagaaatc
    10141 agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct ttacgtttat
    10201 cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta gctctaatca
    10261 atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac tatgcaccgc
    10321 atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat gccttagaag
    10381 aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat atggcaagca
    10441 gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt cgaatcagct
    10501 tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc aagcagcttt
    10561 ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac agtgaaatta
    10621 tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt atcatgcaac
    10681 tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc catgctgatc
    10741 gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt ccaaaactag
    10801 caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa gatgtcgaag
    10861 ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa gaaacattta
    10921 aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa ttgaatcaaa
    10981 cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg cgtcaaccag
    11041 atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat gaaacgatca
    11101 agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg ctttctgggg
    11161 ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa attgaagctg
    11221 ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca aaagatctaa
    11281 cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca tttaccgaga
    11341 ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt actcgtcgaa
    11401 tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc attttgaatg
    11461 gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct atcaatgaag
    11521 taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa atcattgaag
    11581 tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat tgctgtacga
    11641 tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat caatatgaag
    11701 agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag atggaagaga
    11761 tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta taagaggtta
    11821 tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag ctccatgtat
    11881 tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta tcaagaagtt
    11941 atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag agatcaagct
    12001 acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt tgtgacagaa
    12061 gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa tttttgtgaa
    12121 atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga ggttg
//
LOCUS       DAZKOK010000090        12141 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000090 DAZKOK010000000
VERSION     DAZKOK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.090"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..581)
                     /gene="frr"
                     /locus_tag="ACG90W_002105"
     CDS             complement(24..581)
                     /gene="frr"
                     /locus_tag="ACG90W_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0478445.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(584..1306)
                     /gene="pyrH"
                     /locus_tag="ACG90W_002106"
     CDS             complement(584..1306)
                     /gene="pyrH"
                     /locus_tag="ACG90W_002106"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0478446.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1442..2323)
                     /gene="tsf"
                     /locus_tag="ACG90W_002107"
     CDS             complement(1442..2323)
                     /gene="tsf"
                     /locus_tag="ACG90W_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0478447.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2422..3204)
                     /gene="rpsB"
                     /locus_tag="ACG90W_002108"
     CDS             complement(2422..3204)
                     /gene="rpsB"
                     /locus_tag="ACG90W_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0478448.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3563..4042
                     /locus_tag="ACG90W_002109"
     CDS             3563..4042
                     /locus_tag="ACG90W_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0478449.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4259..5350
                     /gene="phnW"
                     /locus_tag="ACG90W_002110"
     CDS             4259..5350
                     /gene="phnW"
                     /locus_tag="ACG90W_002110"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0478450.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5343..5471
                     /locus_tag="ACG90W_002111"
     CDS             5343..5471
                     /locus_tag="ACG90W_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0478451.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5475..6020
                     /locus_tag="ACG90W_002112"
     CDS             5475..6020
                     /locus_tag="ACG90W_002112"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0478452.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6474..8165)
                     /gene="argS"
                     /locus_tag="ACG90W_002113"
     CDS             complement(6474..8165)
                     /gene="argS"
                     /locus_tag="ACG90W_002113"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0478453.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8585..9532)
                     /gene="arcC"
                     /locus_tag="ACG90W_002114"
     CDS             complement(8585..9532)
                     /gene="arcC"
                     /locus_tag="ACG90W_002114"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0478454.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9647..10666)
                     /gene="argF"
                     /locus_tag="ACG90W_002115"
     CDS             complement(9647..10666)
                     /gene="argF"
                     /locus_tag="ACG90W_002115"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0478455.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10757..11986)
                     /gene="arcA"
                     /locus_tag="ACG90W_002116"
     CDS             complement(10757..11986)
                     /gene="arcA"
                     /locus_tag="ACG90W_002116"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0478456.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3314 a         2524 c         2195 g         4108 t
ORIGIN      
        1 gcccctctgt ttttatgatt ttattaaact tccaataatt ctttttcttt atcagcagcg
       61 atcttatcta cttctttgat gctgttatct gtcaatgttt gtacgtcttt ttctaatccg
      121 cgcaaatcat cttcggtaat atcaccattt ttttgttgtt ttttatattc atcgatcgca
      181 tcacgacgga tgttacgaat cgcgattttt gcattttctg cttctttttt cacatcttta
      241 gctaattctt ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat gacatttcca
      301 tcattggttg gactgattcc gatatcactt gccataatag ctttttcaac atcttgaaga
      361 atgctcttgt caaaaggtgt gatcattaaa acacgtgctt ctggaatttg gattgatgcc
      421 atttggttca atggtgttgg cgcaccgtag tagttcacag taatacggtc caaaaggcta
      481 gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt gaagattttg tgctgctttt
      541 ttcatttttt cttttgcttc tgtcataatt gcatctgcca tttttatttc ccccttacag
      601 ttgttccgat attttcgcct aagattgcac ggcgaatatt tccgtgttca tttaaattga
      661 agacaagcag tggaatatca ttatccatgc ttagtgaact tgcagttgaa tccatgactt
      721 gtaatccttt ggcaataact tccagatgag tcagttcttc aaattttact gcatttgcat
      781 ctactttcgg atcagcagaa taaacaccgt ctacattgtt tttagccatc aggataacat
      841 ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc tgtcgagaag tacgggttgc
      901 cggtaccgcc tgcaaagata acgatccgtc ctttttctaa atgacgttct gctttacggc
      961 gaatatatgg ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt gtcggtacac
     1021 ccacgttttc caacgtatcc tgtaaagcta aggcattcat aactgtagct aacataccca
     1081 tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc gatttgtccg cgccagatat
     1141 ttccgccgcc cacaacgatt gccatttcaa cgcccagctc gtgaacttct ttgatttctt
     1201 ggatgatttc tttgattact ggtggtttga ttccaaatcc tgcgtcccca gctaaagctt
     1261 ctccactcaa ttttaagact acacgctgat atttcggtgt gaccatgttc attcctccaa
     1321 tattttctat tgccatttta ccatacttag atgatttcga cactctaaga ctggtttttc
     1381 ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt attcaacaga
     1441 attatttttt cacttggctc ataacttctt ctacaaagtt atcttcgcgt ttttcgatac
     1501 cttcgccaac ttcgaaacgt acgaatgttt taacagttgc gccttttgat gcaacgtatt
     1561 tttcaacagt catatcagga tctttaacga atggttggtc caccaaagcg atttctgctt
     1621 tgaatttctt caagcgtcct tctaccattt tttcaacgat attcgctggt ttgccttcgt
     1681 tcaatgcttg ttctgtaaga acagtttttt catgttctaa ttcagcttct ggaatttgtg
     1741 attcgtttac ataacgagga ttgatagctg ctacgtgcat agctacgtct cttgcaactg
     1801 tttcgtcagt tgttccatct agaacagtca atacggcaat acgtccgccc atatgcaagt
     1861 atccaccaaa tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg aagctgattt
     1921 tttctccgat aacttgagtt gcttcgatca agtcagattc aattgtacct ttatctgttt
     1981 tgattttcat tgcagcttcc atgtctgctg gtttgttttc agcaacaagt tctgcaattt
     2041 cttttactaa atcttggaac atttcgtttt tagaaacaaa gtctgtttct gagtttactt
     2101 caacgattgc tgcagtgtta cctttcacag caactgaagc caatccttca gctgcaatgc
     2161 ggtcattttt cttcgcagcc tttgccattc ctttttcacg taaaagatca actgcttttt
     2221 ccatgtcgcc ttctacttca actaatgctt ttttcgcatc catcattcct acgccagtca
     2281 tttcgcgtag ttcttttacc attttagctg taacgtctgc catgtttaac tcctcctgtg
     2341 tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc ccagcctttg
     2401 aaacagctcc atcattcaaa attattctgc tgaagaattg tcgccttcaa caacatctac
     2461 gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa ctacttgatc
     2521 ttcaccttgg tttccttcga tgaaagcatc agccattttt gaagtaatca atttaacggc
     2581 acgaattgca tcgtcgtttg aagggattac tacgtcgatt tcatctggat cgcagtttgt
     2641 atcaaccata gctacgattg ggatatttaa tttgtgtgct tcttgaacag caatacgttc
     2701 tttacgtggg tcaacgatgt acattacatc tgggattctt ggcatatcag cgataccgcc
     2761 taagaatttt tcaagacgtt cacgttcttt gttcaaacca gcaacttctt ttttaggtag
     2821 aacttcaaaa gttccatctt cttccattgc attgatttgt ttcaaacggc tgatacgttt
     2881 ttggatagta tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa cgaagtattg
     2941 tcctgcacga gttgcttctt ctttgattgc ttcttgtgct tgtttttttg tacctacgaa
     3001 taatgctacg ccgccttctt cagcaacgct cttcatgtaa tcataagctg catctactaa
     3061 tttaactgtt ttttgcaagt caatgatgta gattccgttt ctttctgtga agatatattt
     3121 cttcattttt ggattccagc gacgagtttg gtgaccaaag tgtacgccgg cttctagtaa
     3181 ttgtttcata gaaattactg ccatgtttgt ttcctccgat ttggttttat ttttcctctt
     3241 cttatcttct accttgccgg caaacttttt caagcaccta ccgacaatcc aataagaatg
     3301 tggatttgct gatgcataaa gcaccctcag ctattataca gacaaaagat ccgtatcgca
     3361 agagcaaata tacttttctg ctcaacattc aatagtttac ctgaaagata aaaagaattc
     3421 aagaaaaaca gtagatttct ctttgtttct ttattatcct ttttccataa aataatttta
     3481 ggtataaaag acgaatatta ttgggcaatc cttctttttt ttgttatgat acatgtgaaa
     3541 tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga catttgctga ttaaacaagt
     3601 cattgaagaa ttcccgatcc gtacgcaaga agaactatta aacaaacttg ggcattatgg
     3661 cgttaccgcc acgcaggcaa cgatttctcg agatatccgt gatctaaaaa tcgtgaaggc
     3721 acctgatgaa aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa ctcctgagac
     3781 taggactgag gaagaggatc ggctggttca aatgattgaa gacattgtat tgaaagtcga
     3841 acgagtccac tttttgacga tcgtcaatac cttgccagac aatgctcatt tatttgcatc
     3901 tgttttagat gagatcaaac ctccctctat tgtcagtaca attgctggat tcgatacgat
     3961 catggtcatc tcggaaaatg aggaggacgc aaaacgagta gaagaatttc ttcaccatcc
     4021 aactgaagta tcactttttt aatcagagca cgtattccta atatggaatg cgtgtttttt
     4081 tattttcaat attttcctta ttgaataaac ctttacactc tttacaaaaa tacatcattt
     4141 tttattcaaa tttttcaaca ccttgattga atcacaataa tcatcattta tattggatgt
     4201 taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag gaaatcatat
     4261 gtataaatta ttaacttctg gaccattgac gacttctgaa acagtgaaaa atgctatgct
     4321 gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga taagaaaaaa
     4381 attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa tgcaaggtag
     4441 cagctccttc ggggtagaat cggtattaag ttctgtgatc aggccagaag atactgtact
     4501 gatttgttca aacggcgcat atagacaacg aatgatcaac atgtgcgaat gtctgaaact
     4561 gaactatacc gtttatgagg tatcggaaca tcaaatcctc caagctcatg tgattacaca
     4621 gctgattgaa gagaatccgt ctatctccgc actagcaatg gtccatagcg agactacttc
     4681 cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaagag atcttttgtt
     4741 tatagtggac gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg aactagggat
     4801 tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct tttcttttgt
     4861 acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt tgtcacttga
     4921 tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt tcacttcccc
     4981 tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag aaggaggaat
     5041 cgaaactcga tattcacgtt atctcgccaa taatcatgtg ctacgacaaa aaatgacaga
     5101 gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct cttttcttta
     5161 ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc aaaatggatt
     5221 tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa atattggcga
     5281 gatctatccg gaagatattt tggaactgac agcaattatc aaagactata aggagaacgt
     5341 taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt ggctgtatgg
     5401 ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aacccaactg actgacaaag
     5461 aaatccgcta accgatggga aaattaaaat gggatcatat ccaacagctt ctttccctcc
     5521 cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa aaagaggtgg
     5581 aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag cattctacct
     5641 tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc aatattggtt
     5701 ccacgaatgg atttaatgct cctatgatga caatcgtagc agaaaccgca aaaaaagaaa
     5761 ggtattcacc agacttcatg gcaacttttg aagacgtcga agggtatggt cgaccgtttc
     5821 cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat gaagtaatca
     5881 aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg ttgacagtag
     5941 gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa aaattagaga
     6001 tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag ccggcgctga
     6061 tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg acaagaatcc
     6121 agaatgactt ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc aagtaataaa
     6181 aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttaggcttat taccgcagat
     6241 gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac gacttttgtc
     6301 acagtcccct tatttgcaaa caaacagtgt ttaagagaca acctctccac taaacggtgt
     6361 tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa gctattttct
     6421 cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt ttcttacatc
     6481 ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac agtcactgca
     6541 taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt cgtatgagca
     6601 tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac tgacggttcg
     6661 tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt aaccacttcc
     6721 cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa tcctgctttt
     6781 tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt ttctccttca
     6841 aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt tttcaaatcg
     6901 tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt ttccaaatca
     6961 gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa tacttcttct
     7021 aataagacga tctttccttt acgagttgat aattttttac caccttgcgt gatcaaacca
     7081 aatggaatat ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa gacagctttc
     7141 aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg tttgaagtcg
     7201 taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag tgtcgcaccg
     7261 tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac gatctctgca
     7321 cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc atccatcttg
     7381 tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag atcatagatt
     7441 ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat agcctcttta
     7501 tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc tggttctgtt
     7561 tcagctactt catggaattg gacgtataaa cgaagcaatt cattgattgg ttctgcttta
     7621 acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa ctgtgttccc
     7681 cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat aatgaaaccg
     7741 atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt ggcaatatta
     7801 ggagaagaca tgtcgattgg tactgttcct tgattcccga tgttgctatc cccataatgt
     7861 tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt attcatgaaa
     7921 aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc gattttttct
     7981 gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa tgaaaaggct
     8041 ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg ctcgacttgt
     8101 tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag atctttatta
     8161 ttcatcttta ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa tctctagcga
     8221 cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac tccaattgtc
     8281 ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa aacagaattc
     8341 tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga attcgctagc
     8401 caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa ctttcttgaa
     8461 ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa aaaaaaaatc cagatggcct
     8521 aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa
     8581 caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt
     8641 ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgctt
     8701 ctactttcgg aagcatgctt cctggagcga attgcttttc atcaatgtat tgtttcattt
     8761 ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat
     8821 tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg
     8881 aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa
     8941 caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga
     9001 tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac
     9061 gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt
     9121 cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat
     9181 ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta
     9241 cttcgcccat ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg
     9301 tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt
     9361 taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt
     9421 tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg
     9481 tagacaagat ggcattgccc cctaaagcga caactacttt tcgtttggcc atttcattcg
     9541 tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt
     9601 tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga
     9661 taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg
     9721 cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag
     9781 tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg
     9841 gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat
     9901 tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg
     9961 atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg
    10021 taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt
    10081 cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg
    10141 tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac
    10201 cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat
    10261 gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta
    10321 ccattttttg actgaatcca cggaattcga ttccgtcaaa catacgtcct agtactttag
    10381 ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct agatattcag
    10441 gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt
    10501 tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt
    10561 tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt
    10621 ctttttctgc taataagctt cttccttgga atactgattc tttcatatta aacattctcc
    10681 tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagact
    10741 gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg
    10801 gaccaccacg acctcgtgac aattcacttg aattgatttc taacactttg atgccatagc
    10861 tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga
    10921 ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat
    10981 taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta
    11041 agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca
    11101 tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg
    11161 taaatacggt atccaaatgc atcattgcac ggttgttcgg aattttgatt gccaacactt
    11221 tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat
    11281 tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc
    11341 cttcgatatg atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc
    11401 ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag
    11461 tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca
    11521 agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcatatagac
    11581 tgctagaacg tacttcgata tcttttgtac gaaccccagc catgatttta tctaccattt
    11641 cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa
    11701 tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg
    11761 cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtcttcg
    11821 caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac
    11881 gatccataat atctggtgtc aaattttcca cttcttgtcc aggccgtttc agtaaaactg
    11941 tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc
    12001 ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct
    12061 cattcaaaat gagacattcc tcacattaat attcgataat taaataatta aacgtttttc
    12121 tatttttaag cgtaatttat t
//
LOCUS       DAZKOK010000091        12028 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000091 DAZKOK010000000
VERSION     DAZKOK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.091"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG90W_002117"
     CDS             140..460
                     /locus_tag="ACG90W_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478457.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG90W_002118"
     CDS             912..1112
                     /locus_tag="ACG90W_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0478458.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG90W_002119"
     CDS             1259..4048
                     /locus_tag="ACG90W_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0478459.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG90W_002120"
     CDS             4095..4622
                     /locus_tag="ACG90W_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0478460.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG90W_002121"
     CDS             4643..5833
                     /locus_tag="ACG90W_002121"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0478461.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG90W_002122"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG90W_002122"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0478462.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG90W_002123"
     CDS             7966..8640
                     /locus_tag="ACG90W_002123"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0478463.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG90W_002124"
     CDS             8637..8978
                     /locus_tag="ACG90W_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478464.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG90W_002125"
     CDS             9008..9367
                     /locus_tag="ACG90W_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0478465.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG90W_002126"
     CDS             9627..9749
                     /locus_tag="ACG90W_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478466.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG90W_002127"
     CDS             9891..11975
                     /locus_tag="ACG90W_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0478467.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4003 a         2108 c         2593 g         3324 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatg
//
LOCUS       DAZKOK010000092        11829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000092 DAZKOK010000000
VERSION     DAZKOK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.092"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..314)
                     /locus_tag="ACG90W_002128"
     CDS             complement(<1..314)
                     /locus_tag="ACG90W_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0478468.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     ML"
     gene            complement(501..1151)
                     /locus_tag="ACG90W_002129"
     CDS             complement(501..1151)
                     /locus_tag="ACG90W_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478469.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1694..2629)
                     /gene="whiA"
                     /locus_tag="ACG90W_002130"
     CDS             complement(1694..2629)
                     /gene="whiA"
                     /locus_tag="ACG90W_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0478470.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2663..3661)
                     /gene="yvcK"
                     /locus_tag="ACG90W_002131"
     CDS             complement(2663..3661)
                     /gene="yvcK"
                     /locus_tag="ACG90W_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0478471.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3658..4542)
                     /gene="rapZ"
                     /locus_tag="ACG90W_002132"
     CDS             complement(3658..4542)
                     /gene="rapZ"
                     /locus_tag="ACG90W_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0478472.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4677..5408)
                     /locus_tag="ACG90W_002133"
     CDS             complement(4677..5408)
                     /locus_tag="ACG90W_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0478473.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5412..6677)
                     /locus_tag="ACG90W_002134"
     CDS             complement(5412..6677)
                     /locus_tag="ACG90W_002134"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0478474.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(6940..9756)
                     /gene="uvrA"
                     /locus_tag="ACG90W_002135"
     CDS             complement(6940..9756)
                     /gene="uvrA"
                     /locus_tag="ACG90W_002135"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0478475.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9766..11760)
                     /gene="uvrB"
                     /locus_tag="ACG90W_002136"
     CDS             complement(9766..11760)
                     /gene="uvrB"
                     /locus_tag="ACG90W_002136"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0478476.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
BASE COUNT         3361 a         2628 c         1934 g         3906 t
ORIGIN      
        1 tctagcatag gaccgacaat tgaagaagtc actactgcaa atgctggacc tatccaagca
       61 acattagaat gttctttctc tcttttcctt tgtaaatctt tgttgagtat taatctattt
      121 atttcttctg caggcaagtt aattttcata accttatggg ggattatcca attaaaaatc
      181 ggaaaaaggt agcctcccca aaaagtatag tgatctgtgt caatcagata ataacctgat
      241 tttgattcca gaataacata tctcaaagcc gaacgaaaat ttttcatgaa tatccataat
      301 ctcactgttt ccacacctaa ttcctccaat tccaattaaa tggatttata gcatcaccaa
      361 ttatgttaac tgcctgacca acctcattga taaaattggc accactatcc catgcttcaa
      421 caatgctata tatctatcaa acaaaaaatg gaaaactaaa tactcgcgca cataaatttt
      481 acattctgtt ttttataaga ctaataataa tcattgaaaa aatttcttat cggtggcgca
      541 tattcccaat tgattaatgt gatcccatat tctgcaagta ttttacgagc ttcttctaag
      601 tcatagtgag aaaaatgcat aaaacctgca atacgatcac ggactgtgag ctgtggcttg
      661 cttccaccat cattcataaa atatccagca gagccactac ttaaagtgat tgttacatct
      721 tcactttcta ttattaagaa ttcataatga aatgctttca actctccact attatttaaa
      781 agttttatac tccaacttcg tctaggttga gttgtcacat tccctctaaa acttcctata
      841 aattctgcaa tcaccgtatc ttcaatcaaa attctttcct catctatttc atattcaaga
      901 ctaaacggaa attctccata tgtaatctct ggcaatggtg gtgctgtagt tctccaaata
      961 aatacaataa ctgtgaatag aaaaaatcca acgatcgata taaatgttat gagacctgct
     1021 cgaagtaaaa cccgatataa taaaaatgct gtatttcgct ttgctatatc tagaaaaata
     1081 aaaagtactg caatcataat gatagtaaaa gttgtaaaaa agcacatttt ttgccctccc
     1141 tttataaaca tcgattctca aattctaaac caaatcttga tagttcaagt tcaggtataa
     1201 cgtataccaa ccatacatcc caattactat ttatgaactt atgattatca ctgaataaat
     1261 cactccatcc ccttgttaaa acttctacat taattacacc tggttgtttt cgactagcta
     1321 atccaatgaa cttatcaacc atccttatcc accaaccacg agaatcttga ccagaagtaa
     1381 gaaaaacagt tctcaaggtt gatcgagcag ttccttccag tttttaatta atctccagtt
     1441 atagataaat acttgatcct atctatatca ggacttctat attaagcaaa agtttatgta
     1501 taaaactaaa atcttcattc agttgtctcc ccgcttttta tacgattgta ttaaaaaaca
     1561 atagaagcct ttcaacatta tgttactcca gtaaagttgg aattatagtt tgacggaaga
     1621 aaagcaagta tagcttttaa aaaaaactga tacattcgca tctcctgctt catgtatcag
     1681 ttttttcttt ggattaaact gcttcttcac gcagtttttc cgcaaattca tttattttgc
     1741 gaattcggtg attaattccc gattttgata ttgcacctga gggaatcatt tcccctaatt
     1801 ctttcaagct gacctctgga tgttgaagcc gaagttctgc gatttcctga agtttttcag
     1861 gaagggcatg tagaccgacc tttgcttcga tcaattcgat gttttcgatt tgtttagaag
     1921 ccgcatcaat cgtcttgttc agatttgctg tctcacaatt gactagacga ttgaccgaat
     1981 ttctcatatc ccgtacgatt cgaacatctt caaatcggag cattgagttg gttgcaccaa
     2041 tcaatgttaa gaaatcagca attttttctg ctccttttaa atacgaaata tagccattgc
     2101 gccgttcaag cgttcgagca tgtagaccgt aataatttaa catatcgcaa atgtcttggt
     2161 tatgctgttc ataaatagaa aatatttcca aatgataacg actcgtttcc ggattattga
     2221 ctgaacctga ggccataaaa gcaccacgta agtaagaacg cattttttgc gaatttccca
     2281 taatctcgtc cgaaacgtgt ccattgaaca tcataccatc catgatatca agatccatca
     2341 acacatcttt tgtgccttgc ttcaaccgga cgatatagac attgttcttt tttaatttca
     2401 tttttttacg aacgagtagc tcacttcgta cctgataatg atctttcaaa agagaataga
     2461 tacgtctggc aattgcagca ttttctgttt ggacgttcaa aataaactgc tggttcacaa
     2521 tacctaaaga gccgttcatt cgcagcaaag cagctaattc cgctttcgca tgttctcgat
     2581 gaacttccaa accagttagt tctttcttca catctgcagc aaatgacatc ctattctcct
     2641 cctttctcca acaataaatt gcttaatatt ttgaaccgaa aactaaacgg aacaattctt
     2701 ctactacttt gtctccatcg tggaagacac caccatcttt caattctaaa aagtcggtgg
     2761 aaatcactcg acatccttca tttctcaatc cgttgaagtc gtgtgcgact tgtactagat
     2821 actcatcata gatctctggg tccatataat cttcaggcac tttttccgta ttgaccaata
     2881 ctgtatcgat aaaaggctga ttcaaatgct tgtgtaaaac acgtatatga tcggcatctg
     2941 tgaaatgttc cgtttcccct ttttgggtca tgatattaca gatatagacg gtttcagcag
     3001 ttgtcttgag cattgcttcg ccgatttctg gtatcactaa atttggaagg atactggtga
     3061 ataaacttcc gggtcccaaa acgaccatgt cggcgtccat aattgctgaa acgacttttc
     3121 gagccgcttt ggcttcactt ttatcatttg tgttgcagac atacacacgg tcaatggttt
     3181 tacggttgat cgcgatttta gattctccta ctgcagtcgt tccgtcttta aaaacagcat
     3241 gcaaggtcaa aggagtttct gaagaaggat aaacatgtcc atctacatgc atcatttttg
     3301 ctaataattg aatcgcctca taagtgcttc cccgcatctc ggaaactgcg gcaatgatca
     3361 gattaccgat cgtatgattg gctaaaaact gatcttcctt tttgaaacgg tattggaaaa
     3421 tatcttcata gaattgtggc atatccgata acgccactaa gacatttctc aagtcaccag
     3481 gaggagccat agcgatcgat tctctgattg caccgctgct tccaccatca tctgcaacag
     3541 tcactactgc tgtgatatct gctccttgat ttcgtaaact tttcaggata actggcagac
     3601 cagtgccacc gccgaccact acgatctttg gttttcttat ccggtaggtt ttcattttca
     3661 cgaccggttc accgtctctt tccttttatc cttgtcccga tgtgtcacat tgactttata
     3721 gtttttccct aaagcatttg ccaaacgttc agtcaatgcc acagaacggt gttgaccgcc
     3781 tgtacagccg attgcaactg tcaagctgct ttttccttct ttaatgtatc caggcaatac
     3841 agtttccaga agatccaaaa atttatggta gaatgattgc gtttcatcaa aggacatcac
     3901 ataatcatag actggctgat cttttccggt caatggtctt agttccggga tataatgagg
     3961 gttgggcaaa aaacgcacat ccatcacaat atccgaatcg atcggcaacc cgtatttaaa
     4021 accaaatgat acaaattcga tccgaaatcc ttggtctccc tttgatttga atgactcatt
     4081 gattttttca cgtaattgtc gtggtgtcaa tgttgtcgta tcaatgatca cggaagcttg
     4141 ggctttcaag tcgtctagta tcgcccgttc tttacgtatc ccttcagtga tcaaaccgtc
     4201 catcgccatt ggatgtgctc gtcttgtttc tttgtagcga gccactagtt cttcgtctga
     4261 agcatctaaa aataagatag aagtgtcgat gaagttcgta ttttcgattt cgatcagcat
     4321 gttttggatt tcttcaaaga aggaacgaga acgcaaatcc actcctaaag ccatttttgt
     4381 gactttacct gattctttga tcaattccca gaattttgga atcaatcgag gcggcatatt
     4441 atcgatacaa aaatagccca tatcttcaaa actttggata gctactgttt tccctgcacc
     4501 actcatacct gttatgatga ctaattgcaa attatctgtc atatttacga acccctctca
     4561 cattgtgcat aataaaatta tatcatcccg ggcgtatcat taccagtttt ctttttcttt
     4621 tacaaaaaga tttggaccaa atatctttta ggatatttgc cccaaatcca atttttttac
     4681 ttttccgatg ctgtatccaa tgcctcgctt ctttcagcta atgcacgttc gatcgttcga
     4741 tccgctaaaa tagattgagc atcgatcact gggtaatgat tatcttcagc aaattcatgc
     4801 atcaaagata attctgtgca acctaggatg actttttcac agtttaggcg ctctaccgct
     4861 tcctctaaga tttcgtaata gagctcttga ttcaaataat ccgattcctt gatctcatga
     4921 tagatcaggt aattgatttt ttcttgaagc gcagtatcag gaatcactgt ttcgaaacca
     4981 agatttttga cttcgcgttc atagattcca gctttcatac ttccttctgt tcctagaatc
     5041 gctactctcc ctgtcgtatg ctgacggaca agttcatttg ctgcttctct tggcatatgc
     5101 aagataggaa tatccgttgc cgcttgtaac tcttcaaaaa agtagtgtgc ggtgttacaa
     5161 gtcaacacaa tgaagtttgg tcgcaataaa ttttgtttct caatatcatc cagcagaaac
     5221 ggcattggat tttcttctga tcgatctaaa atataagctg tacgatcagg aactgttgca
     5281 tgattgaata agacataatt caaatattct tgatcttttg tagctttcgt tcggtggttg
     5341 atcaagcgaa caaaactttc tgtcgccatc gtgcccattc cgcctaaaat actgaaaaaa
     5401 ttctccatga actagccttc tttcacatga aaatactttt tgaatcttgg aatatagtta
     5461 tggaacatat atttcatcaa caaccagcgc ttgattgatc ggtctttttc ataaaataca
     5521 gtagtaccgt aacgattttc tttgatcatt tgctcagcta gttttttgtc ttcattttcc
     5581 cgagcatatt ctaaaaagat ttttttgggc acacccatcc ataatcttgc tttatctgat
     5641 tgattcgttc cataggtcgt ttcaacaaag ggtttgttga acactcgatc ttctgtgaca
     5701 aaacgagcca gattcaagcc gttcaatgtc acaaagaaac tgctacgacc ttgtctcaaa
     5761 ttgatttcaa ataatttata ttctccatct ctaggatcat acttcatatc gaaattggca
     5821 aagcctgtat attcgatttt ttccaaaaat gccttaatgg tttgatagat tttttcgtta
     5881 taatctggca tgatcacaac gtaattacca atcgatgctg gtgtaggatc ttctaatagt
     5941 ggatgaccaa ggcacatcat ccgaacttga tgatcttcat ctacatacgc atttagcaca
     6001 cgcatattac tatcatcacc cgggataaaa tcttgtacaa tcatttcact tttataaccg
     6061 gcttcgtata tccggcctaa aattaaatcg aactcttctc tagtctctaa aataaaagct
     6121 ttttttcttc cttcaaattg gacagaaaga tactccacac tgtttgcagg tttcaaagca
     6181 actgggaatt caaaaggcaa ttcctgttcc aaatgtccat ttactaacat ttcttctctg
     6241 acgatcaatg ttttaggata cggaagatca tattcttcac aaacttcata aaagcttact
     6301 ttattgatca gcctttcaaa taaagaataa tcaatatatg gacagataaa atattctgac
     6361 agctcttgtt tatgttggga gatcaattct gcatatccat caccacaagc aatcaaaaga
     6421 tattttttac tcttatcaga atatctttct tttgccaaac gaagcatcgt ctcgataaat
     6481 acagggtctt tatcaaatcc tggaataact tctacattga caattttact gtagcgggta
     6541 ggggctaact gatccgaagc atatgcttgg cagattttgc cgtatgcttc attaaaagaa
     6601 cgtgccatac cataaacgtt catgtcgctt cctaataata tagggataaa ttcttcattt
     6661 tcaatactgt tcatcattat ctctcactta ctcataatct cttgtttttc ttttccaact
     6721 acacaaggaa tatcatacca taaagaaaaa agcaatcaaa ggaaagtcct ccgattgctc
     6781 aattcttttt tgaacggatt gaacttatag gaagttttta tctataccaa tcagtgtaga
     6841 aagaactacc atgtaatgat tctagaacac tgatattaat aaaacaagca aaaattcatt
     6901 tttttatgtt tttttaacta tctcagctat ctatcctctt tataccatta tttttttcaa
     6961 atagtgacct gtataacttt cttttacgtt aatgatttct tctggtgttc cagtcgcaag
     7021 gatcgttcct ccaccttctc caccttctgg tcctaaatca atgatataat ctgctgtttt
     7081 gattacatct agattgtgtt caatcactaa aactgtgtta ccagcatcta ctaatctttg
     7141 taatacatgc aacaagcggg cgatgtcatc gctatgaagt cctgtcgttg gttcatctag
     7201 tatatagaaa ttctttccat tagagatttt gtgcaattca ctggcaagtt tcatccgttg
     7261 tgcctcacca ccggacaatg tcgttgctgg ttgccccatc gtcacataac ctaagccaac
     7321 atcaacaatc gtttgcagtt tgcgatgaat ctttggaatg tgcttgaaga attctacagc
     7381 atcctctact gtcatttcca aaatatcagc aatgcttttt cctttgtaat gcacttctaa
     7441 tgtttcagaa ttatatcgtt taccatgaca tacttcacaa ggaacataga catcaggcaa
     7501 aaagtgcatt tcgatcttaa taattccatc cccgcgacaa gcttcacaac gaccgccttt
     7561 tacgttgaaa ctaaaacgcc ctttcttata accccgcatt tttgcctcgt tcgtttgagc
     7621 aaataaccca cggatatcat caaatacact tgtataagtc gctggattac ttctcggcgt
     7681 ccggccgatt gggctttgat cgatatcgat gatcttttcg atactttcgt agccggaaat
     7741 tgtcttgaat ttacctggct tagcagaatt cttatttaat ttttgcgcta acgatttctt
     7801 taagatacta ttgaccaatg tactctttcc agaaccagac acacctgtga ctgctacaaa
     7861 ttctcctaat ggaaattcaa cactaatatt tttcagattg ttttctgaag caccagtgat
     7921 cttgattgct ttcccattac ctttacggcg ttcttttggt acagggattg attttttccc
     7981 tgaaagatat ttccctgtca aagaattttc atcattagct acttcttcag gagtgcctgc
     8041 agccacgatt tgtccgcctt gttctccggc cccaggtccg acgtcaatca aataatctgc
     8101 tgcccacatc gtatcttcat catgttcgac tacaacgagg gtgttgccaa gatcacgcat
     8161 tttctttaat gagccaatca gtcgatcgtt gtcgcgttga tgcaatccga tcgatggttc
     8221 atctaaaata tacaaaactc cagaaagatt cgatccaatt tgtgtagcca atctgattcg
     8281 ctgcgcttct cccccagata atgttccgga agcgcgactc aaagttagat agtctagtcc
     8341 cacattttcc aagaatgaaa gtctgtcatt tacttctttt aaaatcggtt tggcaattac
     8401 tttctcttgt tcagataacg ttaagtttga gaagaaatct actgcttttt gaatagacaa
     8461 ctcatttgtt tgcccgatat tcgttccatt gatttgaaca gatagagctt ggggattcaa
     8521 tcgataacct ttacatgtct gacaagttaa ttctgtcata taaagacgca tttgctcacg
     8581 cgtgaaatca ctgttcgtat catgataacg acgctcgata ttcttcaata ccccttcgaa
     8641 agtcgtttct acatcgcgaa cgccaccaaa atcattttca taatggaaat ggaaaagatc
     8701 atctgtccca ttcaagatga tctcctgatg ctcttccggt aattcttcga atggtgtatc
     8761 taaatcgata ccgaaacttt cacaagcctg cgctaacatt tgaggataat actgagagct
     8821 gattggattc catggaacga tagcaccttg actaagtgtc ttcgttctat ctggaatcac
     8881 taaatcttga tcgacttcta atttgacacc taaaccatca cagtctggac aagctccaaa
     8941 aggcgcattg aatgagaaca gtcttggttc tagttcgcca acagtaaatc cgcaataagg
     9001 acaggcatag tgttcgctaa acaacatttc ttcttcaccg atcacgtcaa ccagagcata
     9061 gccgtctgct aatcgcaggg ccgcctctaa agaatcaaat aggcgagaac gaatcccttc
     9121 tttcacaatg atccggtcga tgacgatcgc gatatcatgt tttttgtttt tctccagctc
     9181 aggcacttca cttaaatcat agatctcacc atcgactcga acgcggacgt atccctcttt
     9241 ttggattcgt tcgaatattt ttttatgttg tccttttttc tttaccacga tcggtgccaa
     9301 cacttggatc tttgtgcgct caggcaaagc cattacttga tctgccattt gatctactga
     9361 ttgactggta atctcgatat gatcgttggg acaaatcgga tgtcctacac gtgcaaacaa
     9421 caagcgtaaa tagtcattga tttccgtcac agtacctact gtcgaccgag gatttttact
     9481 tgttgttttc tgatcaatag aaatcgccgg actcaatccg tcgatgctat ctacgtccgg
     9541 cttatccatt tgtcctaaaa actgacgagc ataagcagaa aggctctcta cgtatctccg
     9601 ctgcccttca gcgtacagcg tatcaaatgc tagggaactt tttccagaac cggacaaacc
     9661 tgtaacgacc acgaatttat ctcgtggaat cgttacatct atatttttca agttatgggc
     9721 acgtgcacca tgaatcgtaa ttttatcgtt agccatgttg tcctcctact ttgaagcttt
     9781 taattctaaa atcatatctc gcacattcgc ggctgtttca aaatccaaag cttttgccgc
     9841 atctttcatt tctttttcta atttcatcaa taacgtgtct ttttcttcct tcgtcatttc
     9901 ttcgtacgag acttgtaccg cttcttttgt atcatcttcc gattctttcg taatagaaat
     9961 caaatctcgg atttctttaa tgatcgtttt tggaacgatc ccatgttctt cattgtactt
    10021 ctgctgaatc gtacgacgtc ttgacgtttc atccatcgct agacgcatag aatctgttac
    10081 tttgtctgcg tacatgatta ctttaccttc ttcattccga gcagctcgtc caatcgtttg
    10141 gactaaggaa cgttcactac gtaaaaagcc ttctttatcc gcatccaaga tagcaacaag
    10201 ggaaacttca ggtacgtcta atccttcacg aagcaaattg attccaacta acacatcaaa
    10261 ttcacctaat cgaagatctc gaatgatttc tgttcgttcc agcgttttga tatcactatg
    10321 caaatatttg actttgatcc ctaattcttt gaaataatct gtgaggtctt ctgccatttt
    10381 tttcgtcaat gtcgttacaa acacccgctg gtctttttcg actcgttcat ttatttcgcc
    10441 tactagatca tcgatttgtc ccatgattgg acgaacttcg atcactgggt caagcagccc
    10501 tgtcggacgg atgatttgtt ggataacggt atctgtttgt tcatgttcat atggtcctgg
    10561 cgtcgcagag acataaatga tctgattgac atgtttttca aactcttcta accgaagtgg
    10621 ccgattgtct aaagcagatg gcaaacggaa accgtaatct actagcatct gttttctcgc
    10681 tcggtcacca ttatacattc ctctgatttg aggcatggtt acgtgcgatt cgtccgccac
    10741 gatcaaaaaa tcttcaggaa agaaatccaa tagcgtataa ggcggttcac cttctttacg
    10801 tccgtccata tgccgagaat aattttcaat tcccgacgtg tagcccattt ctaacatcat
    10861 ttcaatatcg taattggttc gctgttctaa acgttgagct tccagaagtt tgttttcatt
    10921 tcgcaagaca gttaaacgct gttctaattc ctctttgata ttcgctacgg catgctccat
    10981 atgttcatca ttcgtcacaa agtgagtcgc cgggaagatt gctacgtgtt ccgtttctcc
    11041 taaaacttca cctgtcagtg cattgacttc tcggatacgt tcgacctcat ctccaaaaaa
    11101 ctccacacgt aaggcacgtt catctcttga agccggaaaa atctccacga catcaccgcg
    11161 cactcgaaaa cgaccgcgct gaaaatctat atcatttctt tcgaattgga tactgactaa
    11221 gtcacgaatc aactgattgc gatctagttc tgctccctgt cgaatcgaaa ctacttgttt
    11281 ttggtactca aaaggcgagc ctaatccaaa aatacatgaa acggatgcaa tgacgatcac
    11341 atcatttctt tctaataatg aactagtagc cgaatgtcgt aatttgtcga tctcatcatt
    11401 tacacttgaa tctttttcga tatacgtatc gcttgaaggc acataagctt caggctgata
    11461 ataatcataa taagaaacaa aatattcaac agcattgtta gggaaaaatt ccttgaattc
    11521 cccgtacagc tgaccagcca atgttttgtt atgggcaata atcagtgttg gtttattcac
    11581 tttctcaata agattggaaa tcgtatatgt tttccctgtt ccagtcgcac ctaataagat
    11641 ttgtgctttt ttcccgccga ctacgccatc cactaattgg ttaatcgctt caggctgatc
    11701 accagccggt tggtatttcg agacaagctc aaagtgtcgg gaggtatctc tctcaatcat
    11761 tcttcgtcct ccttttcatt cattcctttt cattttacga ccaataaaaa gaaaaggaaa
    11821 gcaaaggca
//
LOCUS       DAZKOK010000093        11731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000093 DAZKOK010000000
VERSION     DAZKOK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.093"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACG90W_002137"
     CDS             complement(364..1482)
                     /locus_tag="ACG90W_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478477.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACG90W_002138"
     CDS             1715..1837
                     /locus_tag="ACG90W_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478478.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACG90W_002139"
     CDS             1953..3260
                     /locus_tag="ACG90W_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0478479.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACG90W_002140"
     CDS             3379..4578
                     /locus_tag="ACG90W_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0478480.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACG90W_002141"
     CDS             complement(4605..5873)
                     /locus_tag="ACG90W_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0478481.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACG90W_002142"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACG90W_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACG90W_002143"
     CDS             complement(7090..7593)
                     /locus_tag="ACG90W_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0478482.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACG90W_002144"
     CDS             complement(7716..8480)
                     /locus_tag="ACG90W_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0478483.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACG90W_002145"
     CDS             8588..8863
                     /locus_tag="ACG90W_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0478484.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACG90W_002146"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACG90W_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0478485.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10609)
                     /locus_tag="ACG90W_002147"
                     /pseudo
     CDS             complement(10430..10609)
                     /locus_tag="ACG90W_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11435..>11731
                     /locus_tag="ACG90W_002148"
     CDS             11435..>11731
                     /locus_tag="ACG90W_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478486.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3560 a         2449 c         2076 g         3646 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agcaaggtaa
    11581 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
    11641 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
    11701 gcctgacgtc attatcgggc gaaaagaaag g
//
LOCUS       DAZKOK010000094        11614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000094 DAZKOK010000000
VERSION     DAZKOK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.094"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG90W_002149"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG90W_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0478487.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG90W_002150"
     CDS             complement(323..892)
                     /locus_tag="ACG90W_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0478488.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG90W_002151"
     CDS             1078..1980
                     /locus_tag="ACG90W_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0478489.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG90W_002152"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG90W_002152"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0478490.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG90W_002153"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG90W_002153"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0478491.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG90W_002154"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG90W_002154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0478492.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG90W_002155"
     CDS             8554..8739
                     /locus_tag="ACG90W_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0478493.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG90W_002156"
     CDS             complement(8801..9997)
                     /locus_tag="ACG90W_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0478494.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG90W_002157"
     CDS             complement(10167..11603)
                     /locus_tag="ACG90W_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0478495.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3422 a         2480 c         2040 g         3672 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tcca
//
LOCUS       DAZKOK010000095        11441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000095 DAZKOK010000000
VERSION     DAZKOK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.095"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            244..450
                     /locus_tag="ACG90W_002158"
     CDS             244..450
                     /locus_tag="ACG90W_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0478496.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(652..1653)
                     /locus_tag="ACG90W_002159"
     CDS             complement(652..1653)
                     /locus_tag="ACG90W_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0478497.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1658..3571)
                     /locus_tag="ACG90W_002160"
     CDS             complement(1658..3571)
                     /locus_tag="ACG90W_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0478498.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3739..4245)
                     /locus_tag="ACG90W_002161"
     CDS             complement(3739..4245)
                     /locus_tag="ACG90W_002161"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0478499.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4405..4845
                     /locus_tag="ACG90W_002162"
     CDS             4405..4845
                     /locus_tag="ACG90W_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0478500.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4871..5986)
                     /locus_tag="ACG90W_002163"
     CDS             complement(4871..5986)
                     /locus_tag="ACG90W_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478501.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6031..6387)
                     /locus_tag="ACG90W_002164"
     CDS             complement(6031..6387)
                     /locus_tag="ACG90W_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478502.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6685..7659)
                     /gene="sgrA"
                     /locus_tag="ACG90W_002165"
     CDS             complement(6685..7659)
                     /gene="sgrA"
                     /locus_tag="ACG90W_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0478503.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7855..8694)
                     /locus_tag="ACG90W_002166"
     CDS             complement(7855..8694)
                     /locus_tag="ACG90W_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0478504.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8879..9766
                     /locus_tag="ACG90W_002167"
     CDS             8879..9766
                     /locus_tag="ACG90W_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0478505.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10360..11055)
                     /locus_tag="ACG90W_002168"
     CDS             complement(10360..11055)
                     /locus_tag="ACG90W_002168"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0478506.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11039..11437)
                     /locus_tag="ACG90W_002169"
     CDS             complement(11039..11437)
                     /locus_tag="ACG90W_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0478507.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3407 a         2291 c         1713 g         4030 t
ORIGIN      
        1 gggaaattct tttgggggta tcctaaatct tttctattat attcccatat tttctattat
       61 tggtctagac gtatgatggt aaactttgta cctatctgat tgctgaatca ttaattgctt
      121 atcattagcc atctttgttt caagttcgct agatgatcat tttattcaca tcctaacaaa
      181 cttgtcacat ttatagattc ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg
      241 cttatgaaag ctatccatat ttctttgctt tttttcgcta gtgcattgat tttatttgct
      301 attcatcttt gttcccagcc ttatatcaat aacaacggaa tattagtgga accagcattc
      361 ttttgcttac cactcggctt tttgtccctt cttgcctccg caggcttcgg atttgttgct
      421 ttttttaaac gatcaaaaca acaaatctga cttataaaat ttacgtaaaa gagatcgtga
      481 caataatcgt gtgatattcc caaatttcga ataaaaggtg ccccttgcca tctctaactc
      541 ctcagtcaca agttacaata ttcataaacc atgaaaagct tgtttattga aattgctcct
      601 tgtgacttgg agctaagtga cagattacaa cctcttttgc atcataagac tttactcctt
      661 gaaaaacatt actaactttt gatatgtttc ttgcgcttgt ttattgctga aatcaaactg
      721 atattcatgg ttcaaaacag catttttctc cgcataatct acttttgtga ctttcacccc
      781 cgctttctca aatacagaag ttacttcttc catttgtttc gtaaacgtat ttgtatttcc
      841 atccgttaag aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt
      901 gaccttctct gattgttcaa agtccttatt atcaaagtaa gcccatccac atttttcata
      961 aagaaagcta gagattttat cacttacttg agtaagtgca gaaggatcca acagaccaga
     1021 caaagaaata aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt
     1081 tttaatttct tcattttgaa gtgctaacaa atattctcca ctaatttgtg cacctgccga
     1141 atttccacca atcgaaatat tcgaccaatc tgcttgatct tttatctctt ttttagctgc
     1201 ttgaatggct tcgttcaatt ggataactgg tgtaggatgt tttgcttccg gtgatcgttc
     1261 ataatctata tttacgatga tttgctgcgt gtctgaacat agatattcca aataatcacg
     1321 acaatcattt ttatcccctc ctacatatgc gcctccatgc atccagaaaa gaatcggtaa
     1381 ttttttcttt tccactttcg gtgtgtagat atccatatta ttatttttgt actttgaagg
     1441 ataggatatt tcttttttta tatcgatttg ttcgattttt tgctggaaat catctggtcg
     1501 ggcatatact tgttcttttt cacttgtgtc aaattgtttt ctaacaagca agctaatcgg
     1561 ctttggtgtg atttgattaa tcaaaaatac agtgactaaa ataactacga tgataactgg
     1621 tactgcaatc actattcgct tgaaccattt cattcattta ccctctcttt cttcgccaaa
     1681 tccatgtgat aatcgtgcta acagataaaa atccacctga ataaatgaga agcgaatgtt
     1741 tcttttctcc agttttcggc aacattcttt tttttgcatc tgtctttttt tgtactttca
     1801 ctgtcatctg aatcggttgc gtatcatcac ttgattgatc ctctcgaggt tcattctttc
     1861 ccttattgtc tgcattctct gtcggatctt gcttttttgg tggagctact gggatttttt
     1921 tgtaatagaa aataatagtt ggtatcgtct cattaaaaat cccttcagtc ggtccttcaa
     1981 catttgctaa ttcgtatcct tcgaatgtct tttgctctgt catataattt tctccataag
     2041 ctccgtaaag ctggatttcg tcctggtctt tgagtagact tttattgttt tcatccacat
     2101 actttacgtt tatctgatca tggaacacta ctttcaaaaa gtccgttaca tgtttcgtgt
     2161 aggcagcatg gtcaacttcc tgtgcctttc catgccctgc tccttctact aataaaagat
     2221 ctttataccc tcctgataac tcatataatt tttgcgcgtt agacacaggt actacatcat
     2281 ctgctgttcc atggataatc aatttaggca atgaatctga cattccaatc gtaggtagag
     2341 cagcttttgt atccaaatgc atctcctgat caaaatagta ttgtttcaaa aggcccataa
     2401 ataaatcttc atgctcttga tccccaatga tgtctaactg attttttatg gcatccggta
     2461 gtgcgggggc tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc
     2521 cgcaattttc aataattcct ttgacattga caggaatatc tttcgctaat acagacatca
     2581 tcgtagctgc tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa
     2641 cttcttgatt tatccagtaa aggacgtcat ctgactcata ttgtccatag gtcacataat
     2701 cgcctccgct attaccagtc gctctggaat caacaaataa taaattatac ccggccttat
     2761 aaaaatcatt gtattcttct gtaacgatcc cgttattcca attccctcga aatccgccat
     2821 ggatcatgac tgttttatct gaaccttgat cgacataata tgcatataca tccatcattt
     2881 tgccatcttg tttgtttagt acagtttgat gaagttgttt tgtatttgga tagaagcctg
     2941 tgttttctgt aggcgttgtc ccatcatctg tacctccaag catcgacatc aacacactgt
     3001 tttttgcaaa agcgatatcg tacaatagat aagtagcaat cagatgtttg ataatctctt
     3061 tatctacacc ataatcatta acagcaagct cagtgactaa taactctatt aattttgact
     3121 tgatgtcttc tgttggtcca ttaagtattt ctgtcatctg tttaccgaag taattcaaat
     3181 attctttacc attgaaattt tcacctttac cgatatcatc attttctgtc ccattctctt
     3241 tgtcacttac tttagcagaa gaggattcgt cgtttgacat ttttgtagct gttgcttctt
     3301 ctgtttgttg gttatctgtg gtagcggtcg tttcttgatc tgtgctagta acagatgtct
     3361 caaccgttgt ttcagtattt gtttctgttt gtgaagtact cgatttcgtt tcatttccag
     3421 catcagtagt ttctgtggtt tctacctccc gagattccga ttcggaagaa atgctctctc
     3481 catatactgt ccattgcggg acgcttgtac ttacagcaaa taagatgacc aacgctccct
     3541 ttaatacttg ttttgttctc aagctattca ctccttttta tgtttctagg agaattatat
     3601 gtaaacgcaa acatttttta tacaaaaaat aaatggttta tcccataaaa atagaacagg
     3661 attattttct cattttacgt aataaagcag aatggataaa ccagtatcca ttctgctttt
     3721 tttaaacgaa gaaacaactt attgctccaa aaatgtcaga aatcgtttat cccaaatttt
     3781 ctcgtaaaaa gcgattgttt cctttgcttt cataaaagga ttatcataag ttgtcgttgc
     3841 tcctctacac atcataagct ggtatcctaa tccatgagca aacttcacag tcgtatctat
     3901 acaatactct gcttgtgccc cacataattc gatcgaatga cagtccaatt cagttaataa
     3961 aacactcaaa ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa aataactgtg
     4021 ctcaggaata gataattccg acaatagctc ccatggtttt gtttgtggca ctaattgagc
     4081 atcttcgtgt tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt tgattctttt
     4141 attcactaat gctgtcactg tttctaattc gtgtatcggc gaagaatcaa aacatacccc
     4201 tttttgtaaa tcgatcacta ctattgcgtc ggctgtgttt gacatacttg tgccttcttt
     4261 cttttttctt gatttaatag tatttcttat ctagctagtt ccattatacg tgacaacatt
     4321 ttcttatttt acacctctaa atggcacttt tcgactattt cttcatgtta ttatttcata
     4381 aatacttagc tattggagga atgtatgatg attaaattgt atacaggttc tggctgctcg
     4441 tcttcaagac gcgcaaaacg ttggcttatc gtccatgata taccgtttat cgaacagaac
     4501 ttaattcgtc aaccactaac caaaaatgaa tttatccact tactaaaatt gacaaacaac
     4561 ggcgtgaccg aaatgctctc tactcattct attagttata aaaatcttgt tcacagaata
     4621 gatgaactca gtttgagtga actatatatt gagattcgta catccccttc tttattgaaa
     4681 aaaccgatta tctacgacca atctgcaaaa cttttgactg gatttagcga agataaaatc
     4741 aggatgttcc tttctaaaac taaaagaaag caagagctca acgaactctt gctttctggt
     4801 catcttgtcg attttaaaga aaataatctt tttgcaatgt cttaattttg gcataaacaa
     4861 tctgcttttc ttaattaacg aaatccaatt tttccaatat ggatctattt ttctcattcc
     4921 tgatttttat gatttcagct ttcagacgcg cccaatcatt ttttgtaggt tgtccattcc
     4981 agtaaaatat ctgtgtattc gtttgatcat atgccttttt gtcgatagga taatcgctaa
     5041 tgacaatatc aggtaactga tcagcggatg cactatattt tattgggcgt tcagtcatag
     5101 ataatatcct tttctttgcc cataattcct gtgttttacc atactttgat tgaatataga
     5161 atatgacagg ttgttcgtgt tttataaaaa tatccctgac taacatacag tattgaaaaa
     5221 tcaaccgtct gttcttttca taataagtat taagctctgg aattctgcga gctaccccac
     5281 taaaaaactg ttgcatgatt ggatacatca ctggaaatat atgctgaaaa tctctgtctt
     5341 ctgctgattt tccaaaagca tccaccttct tttttgtccc aaaaaccctt gaatacaaat
     5401 ggatcattgc caaattgaca tatagataat ttttttctga agttgtaaaa caaatcttga
     5461 agtttttttc aaaattttct acaatagacg attcaatcca gccccaactt tcttgtactt
     5521 tatctggagt atccaacaaa tcttcttcca aataggtgtt cattacacca aacataaaat
     5581 aaaggtaagc ttgctctgtt ggagtgatgt gattcatcga ccagaagttt gcttcaaaaa
     5641 aaggctctag tatgtaatgc gtaaactctt cttttgttat caatggatgc acaaatttgt
     5701 tcatctcttc tgggacatac gtaatcgagt ggccttgttt cattcgttgc aacgaaattc
     5761 ttagtaggct gatccatttc tttctatcta tactacgcgt atatggcgca tgtttcgcaa
     5821 atttttctaa tagttgacgt ttatttttta tacttgataa tatatcagaa tgattttcgt
     5881 cgaatgactg ccaatacatc aagtgaaaaa agtatcggat acggtattct tctccttcta
     5941 tcggattacc ttttttcaag ctgcaagata aatgatagtt tttcaaaata gtattaagtt
     6001 ctcttaattt tctttgaatc gtacgcaaac ttatataatt ttttgactaa gattttctaa
     6061 ggattgataa gtacctttgt aaagtgaatc cagtaaaata taaccaatcg ttccctctaa
     6121 aagtatagaa taaactttac tcagtaaaaa ctgagaagaa aactcgactt ttatcatctt
     6181 gttttctgaa agaaaagata atttatattc tggatacctt tcgcaaaacg attctgcttg
     6241 ttgaatcacc gaattcatct tagccagggt gtagtttaga tctaaagcga tatccttcac
     6301 cacatataat ttctcgtctc tttcataaca atagttcaaa aattgatata cttgcctttc
     6361 attgatatca ataaattctt caaacaaagt acatactcct ttttatctat tatttagact
     6421 tattccgaca gctacatgaa taacgtacaa atagattcgt tactgatttt tcagagatac
     6481 taccatgcgc agtgtgcatt gtcaattatt cttgctctta ttttccattt gtgttcctgt
     6541 tatttcctgt ttctcaaata tgttagtaaa caaaaataga tctggcaaag ctacaacttc
     6601 gccaggcaat acagcactct cgaatgaaat attcactcaa aaatctatcg ttttattcaa
     6661 ttttagatct gttgctttta ttctttattt cactcgatgg aagagagaac gaaaatagct
     6721 tgctcctaca atcaaaatga gtaacacacc catccatgta attagcttgt tcaaggttct
     6781 actaccagtt tctggtaaat cgctttttct attatctgtt tctttcgctt tttcttgatg
     6841 atcaattcca cgagaaacaa aatcaggctg ttttagcaca ctttttaggt tgctccctgc
     6901 atttctcttt tgttccttag ttggtatgat aggtaccaaa tcaagttcat tatttacttt
     6961 tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc tcactactca tgcttcctgt
     7021 gctactttca ctactcgtac tctccgtact gctttcgctg ctcgtgcttc ctgtgctact
     7081 ttcactactc gtactctccg tactgctttc gctgctcgtg ttttctgtgc tactttcact
     7141 gctcgtactc tctgtactgc tttcaccgct cgtgttttct gtactgcttt cactacttgt
     7201 gctatccgta ctgcttggcg gtccaacaac tgtccaactg cttggtttca aattctccgt
     7261 gatcgtcatc tgtccatcat aaaaaacaat caaatcaact ttatctttac tcttgatctc
     7321 atcaatcgat agttctgcag aaatcattat tgtatactca ataccagcgg tccattgtac
     7381 gtcttttaaa taaacttccc caataacttt ttctgaactt ccattaccat cccaaccata
     7441 tgttagaaca gcatgtacat cactagcatt cacgcttttg tttggtttga ctggaatagt
     7501 aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt ttcacagtaa atgaaagatt
     7561 ctcatttgcc cgttcatttg cagaagtact gattccaatc attgctgcaa ttatactcag
     7621 cccaagaata acaaataata cggtcgctgt tttcttcatt tctatcactc tccatataat
     7681 taccttctat ttgataaagg aattatataa aatgaaaatg atgagaacac gacagtcagt
     7741 tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat ttgtttactt tttcgtttaa
     7801 tcaatgacag acttaaaaaa gcaagaccga tatcagcctt gctttcttca tcttttattg
     7861 ataaacagca gtgtatttct tcatccgctt tgttttatat gcatagagta acaaaccaac
     7921 aatcaagact aacaagaata atgtagttga tatagatgct tcagccccaa agtttccccc
     7981 agtaattatt gtattttgcg attggatcag agtggagaaa aaaccttgtt cttgcgttcc
     8041 gctgactggt atacccaaaa tagctccttg tgtccaattc caagtaatat gagcaccaat
     8101 agccgtaaat aaacttcctg tactgtagat aagtgcgctg aaaaagcaac ctgctaaaaa
     8161 gatattgaaa actgccagaa atgtcatgtt aggattcaat ccgtgcaaaa gagcaaataa
     8221 tgcagcagat ccaatcattg ctattaacgg accaaatcta agcatctgat gctgaaaaaa
     8281 gccacggaat aaaagctctt ctgatgcccc cactgcaata aataaaataa aactcgttat
     8341 cagcggcaca atgtttgcgt ttcctgaatc taaccgatac gcccccgtca aatataatgg
     8401 actagtaata agcagaaaca ctccaattcc aacaacgaga cctcccgcta aataccccca
     8461 gtcttttcct cgaacagaaa caccataatc ttctattctt tttccttcga ccttactagc
     8521 aagaaataaa gctagtaaaa aaacagctaa atgtccgacc gtaccggcaa ttgctccaat
     8581 ccattcacta tttccaataa acatatccgc aagtgcatta ttgacaagaa ttcctaaaac
     8641 aaaaccaact aagactatta aaacagatcc aatcaaccga ataatcctga tcatctttca
     8701 tccctccttt atattaagtt taacaaccct tttctttctt gtctcgtgaa aactatgtaa
     8761 atgttctcca tcatagagag aatagatttt tacgaactaa gaatatctac agcaacctta
     8821 cgaaaatgaa agactgtctc cctaattctt ctgttagact gtttttaata aggaaaggat
     8881 gggaaatttt ttgaaaaaac aactactaat tatgtgcctt tcagcatgtg caattttact
     8941 attctcaggt tgtagcaagt ccaatgaaac gattgcaacg tttaaaggtg gaacacttac
     9001 agttcaggat ttttatgata acccaaaagt taaatcaaat aatcaaacac tattaaaaaa
     9061 aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa gagatttctt caaaggaagt
     9121 aacaaaagaa tataatgaac aaatcaaaaa actcggtcca aactacaagg aacaattaaa
     9181 agcagttggc caaacagaag agacatataa gaaactcttt aaacagatgc tggctttcca
     9241 atacggtttg aaagccaatg taaaattaac agataaagat ctagacactg cttggaaaga
     9301 attctatcca gaagttagta cacagatcat tctattttcg actaaagaag aagcagataa
     9361 agcaaaaaaa gaagcaaatg aaggagaaaa tttctccaag cttgttcaag cttatggaaa
     9421 aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt gattcaacca atccagagat
     9481 tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac ggggaagttt ctgatatcat
     9541 tcctgtaaca gatccaacga cttatcagca aagctattat ctagtaaaaa tggtaaaaaa
     9601 acaggataaa ggttctaata aagacaaata caaatctgag ctagaaaaaa ttgctaccga
     9661 agcaaaaact cttgatacgg aattcatgga taaaacaatc cgtaaagtga tgaaaaaaga
     9721 caatgtaact attaaagatc cttatgtaaa aaatatattc aaataaacaa atttgaccca
     9781 attggggatg tggcaaaatt tgagaagtga tttttggaaa ttccttctta tttttgaggc
     9841 aaaccacttt tgccacaact tcatcggggc ctttttttat ttgtaccaaa acaaatcact
     9901 ggatttgtta caatcatgag gatataattt aaaaaagttt tttatttcta tcacatgaat
     9961 caatgcgcca ttaagtagat tctttcatgc ttccctattt tttcacttgc cgtatcgctt
    10021 caaaaacaac tatcataaac tcaaactcga ctgaaaagtt attttaaaaa agactaaaaa
    10081 tatcccttct ggacttgttc ttcttattct gttatactag agaaacacga aagatatttt
    10141 tatgcgacac ttttcatttg tttttatgaa atttattcaa aactggcgta acttcagagt
    10201 tacaaggatg tattagaggt tagggaatac atcgtttaaa tgagaaataa gtaatagtta
    10261 gtaatagtca cccaaacttt aaattaatga atattgaatc gtcagatagc ctcctcgagt
    10321 tttcgagaag gctatcgttt attttatgga gaaattgatt cagaagtaat cagcgacctt
    10381 ttttagttca ccaatcgttt ctgcttggaa agtttgcacc caatcttctt ggaagcccct
    10441 cactgcttta tcaatcgagg gcatctctaa tacttgatct agtagctctt tacctaacgt
    10501 tatcgcatga gctccagatc gaatggctgt agtgacttgt tcacaatttt tgaaactagc
    10561 tgctaatatt tttgttggtc tttgcgtacg ttcgatctca gtccgcaacg aatcaatcac
    10621 tttcatagca tcaatgcctt gattttgcat cctgttataa taaggtgcaa tataatctgc
    10681 tccattatta atagctaaat acccttgtaa ttctgtgtaa atagctgtac cggtaatcaa
    10741 ataaccttct tttttcaact ttttgatcgc ttgaaggcct gcttgattca ccggcacctt
    10801 gataaacgta tcttttccca gttcttctat gataactcga gcatcctcta tcatttcttc
    10861 ttccgttctt cctattgtct gaatatggat aggaatctgt ccgattattt ttttgatttc
    10921 tttcatatgc gagaagaagt ttgacgctcc ttcttttttc agaattgtag gatttgttgt
    10981 tacgcctgtt aaattcaaaa actgttgaca agaataaata ctttttgtat ttgctgtatc
    11041 aagcatgatt tccatatagc tttcccccat tctgatcctc ttgatcgatt ttaatgattg
    11101 tcccctttct taaatgcggc atttccttat cttctacata cattgttcct ggaagttccg
    11161 gctcatgaga tccatcaaat tttatcgtga tatgtcctaa ttcattcaaa ttagtttgga
    11221 caacatctcc tactgctgtg attacataca tttgtttatc aaaacagagc ttcatatcac
    11281 aagttatttc ttttcttacc tgattcaatt caatcttata acaataatct gttaatgttt
    11341 ccggtgcttc atctccaaat aaaatcatta ttttttcttt cctgaatact tctgcttcta
    11401 taccaacatc tgttacaacc gtttcaaaga tcgtcatctc t
//
LOCUS       DAZKOK010000096        11314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000096 DAZKOK010000000
VERSION     DAZKOK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.096"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            276..1535
                     /gene="lysA"
                     /locus_tag="ACG90W_002170"
     CDS             276..1535
                     /gene="lysA"
                     /locus_tag="ACG90W_002170"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0478508.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1522..2322
                     /gene="dapF"
                     /locus_tag="ACG90W_002171"
     CDS             1522..2322
                     /gene="dapF"
                     /locus_tag="ACG90W_002171"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0478509.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2325..3206
                     /gene="dapA"
                     /locus_tag="ACG90W_002172"
     CDS             2325..3206
                     /gene="dapA"
                     /locus_tag="ACG90W_002172"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0478510.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3203..3919
                     /gene="dapB"
                     /locus_tag="ACG90W_002173"
     CDS             3203..3919
                     /gene="dapB"
                     /locus_tag="ACG90W_002173"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0478511.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3925..4620
                     /gene="dapD"
                     /locus_tag="ACG90W_002174"
     CDS             3925..4620
                     /gene="dapD"
                     /locus_tag="ACG90W_002174"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0478512.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4636..5637
                     /locus_tag="ACG90W_002175"
     CDS             4636..5637
                     /locus_tag="ACG90W_002175"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0478513.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5634..6995
                     /locus_tag="ACG90W_002176"
     CDS             5634..6995
                     /locus_tag="ACG90W_002176"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0478514.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6955..8106
                     /locus_tag="ACG90W_002177"
     CDS             6955..8106
                     /locus_tag="ACG90W_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0478515.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8081..9250
                     /locus_tag="ACG90W_002178"
     CDS             8081..9250
                     /locus_tag="ACG90W_002178"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0478516.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9425..10804
                     /locus_tag="ACG90W_002179"
     CDS             9425..10804
                     /locus_tag="ACG90W_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0478517.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11085..11314
                     /locus_tag="ACG90W_002180"
                     /pseudo
     CDS             11085..11314
                     /locus_tag="ACG90W_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3770 a         1965 c         2514 g         3065 t
ORIGIN      
        1 aattgaaaat atccgaacta ttccgaacta tattgaagcg aaaatatttt ttcatcacta
       61 aatagtgttg aatttatgtt tttatgtata aatagcttct aatagttcgg gtattttgtt
      121 tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact agacaattca
      181 gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg aaattccaat
      241 aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata attggcggag
      301 tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat gatgaagaaa
      361 agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt tttgatacaa
      421 aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta gttcgtgaat
      481 atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg caagctgatt
      541 ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa ctgatctatg
      601 cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt gaattattag
      661 cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg aatgtcggag
      721 tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa ttcggcatga
      781 gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc agctatctag
      841 agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact gcttggttag
      901 ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt tccttgaatc
      961 ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca gtcgaagagg
     1021 cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa ctgaccattc
     1081 gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca acactataca
     1141 cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat ggcgggatga
     1201 cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta gcagcaaaac
     1261 tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa tcaggcgatg
     1321 tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta gctgtttatt
     1381 ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca cgaccagcag
     1441 ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca ttttctgatc
     1501 tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg aaatgatttt
     1561 atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc tttatgcaca
     1621 aaagaaaaat atgatataga cggattgatc gccgtaaaaa tcgatccgtt ggaaatgatc
     1681 tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg ctgttttgct
     1741 cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga gacattagca
     1801 ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt agatatggga
     1861 gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat aaaaaaccag
     1921 gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg aacgattcat
     1981 accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg tgaactgatc
     2041 tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt agtcaatgaa
     2101 caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc ttgcggtaca
     2161 ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc agatcaggta
     2221 gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat acaaatggct
     2281 ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata aaaggttcga
     2341 tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct ggtttggaaa
     2401 aattgatcca attccattta gatgaacaaa cagatggatt attagtttta ggtacaacag
     2461 gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg acagtaaaga
     2521 aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg aaagaaacga
     2581 ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc atcacccctt
     2641 actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt gcagaagcct
     2701 caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg attccaatcc
     2761 atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa gcctcaggag
     2821 atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc ctttacagtg
     2881 gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta ataagtgtat
     2941 gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag gacggaagat
     3001 ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc ttgttcagtg
     3061 agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct tccggacctc
     3121 tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct cagcttttgt
     3181 taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac agatgggaaa
     3241 tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac ggacggcacc
     3301 taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg atttttctca
     3361 tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc cgttagtcat
     3421 tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg ccaaacatgt
     3481 acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc aactaatcaa
     3541 gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac atcatagtcg
     3601 taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc aagaagtgca
     3661 aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc aaattggtgt
     3721 acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg ctgctactga
     3781 tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg caggcgcagt
     3841 agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag aagatttatt
     3901 aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta cattcaaacg
     3961 gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat cgaatttaaa
     4021 gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta tgagacgatt
     4081 gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga aactgctgca
     4141 agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat cgaaccaggt
     4201 gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat gggagcgatt
     4261 atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc tgtcttaggt
     4321 ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt agcaggtgtg
     4381 atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt aggtgcgaat
     4441 gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc aggagcagtg
     4501 gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg tgtattaaaa
     4561 ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg cgaattatag
     4621 aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac cggattagtc
     4681 ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg attgaaatta
     4741 ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa acacactgta
     4801 caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc tgcaggtgct
     4861 tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt catagataat
     4921 tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt caatctggaa
     4981 gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca agcggtactt
     5041 ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc gacctatcaa
     5101 gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca aaatgacgag
     5161 gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat cgatcagcct
     5221 ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagacccg taagattctg
     5281 catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga aaatggacat
     5341 ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat tagagagtgt
     5401 ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa atatccgacg
     5461 agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa agacacttct
     5521 ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg agccgctgcg
     5581 aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc aagatgagta
     5641 atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag caattcaaaa
     5701 aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt tctgctttag
     5761 gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt tatgcttacc
     5821 ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt ttcattcaag
     5881 taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc atacagaaac
     5941 aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa tatctgactg
     6001 ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac ctgattttct
     6061 ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca aaagcattta
     6121 aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca ggaaaagaaa
     6181 aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag atgttgcaag
     6241 cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat ccgcgaatca
     6301 tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag atggcttata
     6361 tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct gatatcccca
     6421 tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatcg aatcacgaaa
     6481 tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt tccatcactt
     6541 tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg agcatttttg
     6601 ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc ggagtgatcg
     6661 tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta aaagaaacac
     6721 ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc gttgggggtc
     6781 cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac aaattagaaa
     6841 ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc ggtgtaccga
     6901 ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat acggatgctg
     6961 aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga attcaagaca
     7021 aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg ttatccatga aatcggcgaa
     7081 acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt tcgaacagat
     7141 atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa gcatgaaggt
     7201 tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt gactgacttt
     7261 ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca gccatcagaa
     7321 gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa atatgaagta
     7381 caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt tttctctcga
     7441 ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg ccagtctgca
     7501 catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg atttttgaag
     7561 aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat gaaatttggc
     7621 caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt atccggaagt
     7681 atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga ccatttagca
     7741 gaacagtttc actctacagc tactatttct tttcgctata atgacggcta tccagctgta
     7801 atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca tgaattagat
     7861 gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc atgtgtattt
     7921 ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga ttttgatatg
     7981 gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga aaatcttctt
     8041 gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaatt tatccaatcg
     8101 atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg atcagcaaat
     8161 atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc caactcctga
     8221 acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact ataccggaat
     8281 gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc gttatggatt
     8341 aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag cgattgcctc
     8401 tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc ctgcttattc
     8461 aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag atacttctga
     8521 cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat atggatcagc
     8581 tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat tgtctgcaaa
     8641 acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta taagtgatga
     8701 agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt atcttccaga
     8761 acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt ggcgagtggg
     8821 ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc aatatctggt
     8881 gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa atggggtgaa
     8941 agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg tccagcaatt
     9001 ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt tttgcaaact
     9061 cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg aacaggcaaa
     9121 agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata tccggatttc
     9181 ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag actttttaac
     9241 agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg tatattctgc
     9301 aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg attcataact
     9361 aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca aggaggtttc
     9421 atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc atcaatcgat
     9481 cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg aagcaggaga
     9541 ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg taacagatgt
     9601 aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc atattttaaa
     9661 taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc
     9721 agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg gtgtcaaacc
     9781 aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc gtggatacaa
     9841 ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt
     9901 aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt
     9961 cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg agatgacaga
    10021 tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga
    10081 agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc
    10141 agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt
    10201 cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga atcttccaga
    10261 tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca aaaaaatgcc
    10321 gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac atctttttga
    10381 agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa gtgtctttga
    10441 ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag gagtagatgt
    10501 acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag aacaaggaaa
    10561 tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc taggaggagc
    10621 agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag cgatccaaaa
    10681 caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag gctttgaccg
    10741 acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac catttgtaga
    10801 ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa
    10861 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta ttaccgcaga
    10921 tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat ttgtgtaccg
    10981 acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggttaaa
    11041 tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa aggcgattgg
    11101 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg gagaagcaca
    11161 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
    11221 aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca tacacgcgta
    11281 aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DAZKOK010000097        11198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000097 DAZKOK010000000
VERSION     DAZKOK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.097"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG90W_002181"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG90W_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0478518.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG90W_002182"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG90W_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0478519.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG90W_002183"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG90W_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0478520.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG90W_002184"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG90W_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0478521.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG90W_002185"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG90W_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0478522.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG90W_002186"
     CDS             complement(6343..7062)
                     /locus_tag="ACG90W_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0478523.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG90W_002187"
     CDS             complement(7059..8372)
                     /locus_tag="ACG90W_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0478524.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG90W_002188"
     CDS             complement(8453..8773)
                     /locus_tag="ACG90W_002188"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0478525.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG90W_002189"
     CDS             complement(8881..9168)
                     /locus_tag="ACG90W_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478526.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG90W_002190"
     CDS             9295..9939
                     /locus_tag="ACG90W_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0478527.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG90W_002191"
     CDS             complement(9998..10705)
                     /locus_tag="ACG90W_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478528.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG90W_002192"
     CDS             complement(10925..11095)
                     /locus_tag="ACG90W_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478529.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAZKOK010000098        10998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000098 DAZKOK010000000
VERSION     DAZKOK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.098"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1061
                     /locus_tag="ACG90W_002193"
     CDS             <1..1061
                     /locus_tag="ACG90W_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0478530.1"
                     /translation="EKEDLNDEINQKYDETLKNVYSSLIVSNIEETSSSEIKNKLSLL
                     KLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKI
                     IKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEE
                     KVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELE
                     VIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEF
                     DVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTF
                     TKLAKAEQYRRSLELSKD"
     gene            1133..2254
                     /locus_tag="ACG90W_002194"
     CDS             1133..2254
                     /locus_tag="ACG90W_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0478531.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2336..4108)
                     /locus_tag="ACG90W_002195"
     CDS             complement(2336..4108)
                     /locus_tag="ACG90W_002195"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478532.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(4111..5823)
                     /locus_tag="ACG90W_002196"
     CDS             complement(4111..5823)
                     /locus_tag="ACG90W_002196"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478533.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5938..6606
                     /locus_tag="ACG90W_002197"
     CDS             5938..6606
                     /locus_tag="ACG90W_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0478534.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6673..7461)
                     /locus_tag="ACG90W_002198"
     CDS             complement(6673..7461)
                     /locus_tag="ACG90W_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0478535.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7544..9016)
                     /locus_tag="ACG90W_002199"
     CDS             complement(7544..9016)
                     /locus_tag="ACG90W_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0478536.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(9146..10339)
                     /gene="metK"
                     /locus_tag="ACG90W_002200"
     CDS             complement(9146..10339)
                     /gene="metK"
                     /locus_tag="ACG90W_002200"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0478537.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10337..10416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10605..10676)
                     /locus_tag="ACG90W_002201"
     tRNA            complement(10605..10676)
                     /locus_tag="ACG90W_002201"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10641..10643),aa:Glu,seq:ttc)
     gene            complement(10688..10761)
                     /locus_tag="ACG90W_002202"
     tRNA            complement(10688..10761)
                     /locus_tag="ACG90W_002202"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10725..10727),aa:Ile,seq:gat)
     gene            complement(10774..10844)
                     /locus_tag="ACG90W_002203"
     tRNA            complement(10774..10844)
                     /locus_tag="ACG90W_002203"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10810..10812),aa:Gly,seq:tcc)
     gene            complement(10849..10921)
                     /locus_tag="ACG90W_002204"
     tRNA            complement(10849..10921)
                     /locus_tag="ACG90W_002204"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10886..10888),aa:Phe,seq:gaa)
     gene            complement(10925..10997)
                     /locus_tag="ACG90W_002205"
     tRNA            complement(10925..10997)
                     /locus_tag="ACG90W_002205"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10962..10964),aa:Asp,seq:gtc)
BASE COUNT         3665 a         2221 c         1821 g         3291 t
ORIGIN      
        1 aagagaaaga agatttaaat gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg
       61 tctattcttc acttatcgtt tcaaatatag aagaaacttc atcttctgaa ataaaaaata
      121 agctttcttt gttaaaatta aaagaaaagg aatatattat tgacgatgct gtttctgtcc
      181 ctcttatttt aaataaacga gagaaaaata atttaaagaa aaaaatgctc attccatttg
      241 attcagaagt gaaaggatta ctcaataaac taactatttc taatatagac tcgactaggg
      301 aaaaaatcat aaaaatattt gataaaatta ataatttatt caagaatgac aatgctgaat
      361 taaggaaggg tcttttagag tttaaacttc aagaactaga acttcattac tcttatttag
      421 tgaaaattac cgaagaaaaa gaacaacaaa aagcaataaa agaacagatg atagaagagg
      481 aaaaagttcg tagagaaatt gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat
      541 tcaattctga aatttccaaa ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa
      601 aagaattata tgcaaataaa attcaagaac tggaagaaaa attaaaagaa ttagaagtta
      661 tacgtgaaaa tgtattacaa agagaattaa atactagagc cggttatgtg tatgtaatta
      721 gtaatattgg gtcttttggt gaagacatat ataaaattgg tatgactaga agattagaac
      781 ctatggatag aataaaagaa ttgagtagtg catcggttcc atttgaattt gacgttcatg
      841 cgatgatttt ttcagaggac gctccgtctt tggaaactaa attgcataat cattttagaa
      901 aacaagaagt taataaaatt aatcaaagaa aagaattttt caaagtttca ctagacgaaa
      961 tagagaaagt tgttttagaa aattataatg gaacagtaac atttacaaaa ttagctaaag
     1021 ctgaacaata tagacgatct ttagaattgt caaaagatta aatatataaa aagccttcgg
     1081 gctttttctt ttaacaatta ataggaacgt atattcgaaa ggaatagaaa taatgtggtt
     1141 agaaaaaata aatgacaaaa aatttaagta caccgaacgt tatacggatc ccttaactga
     1201 aaaaaagaga aaagtgtccg ttactttatc tagcaattca cgccaagctt ggaatcaagc
     1261 taacttactt ttgaatgaaa aaatcgcaga aaaaataaaa cgaaatgaag aattaccact
     1321 aacttttgga gaattaaaaa ctaaatggga tgaaaaatac aagccaactg taaaagaaag
     1381 ttcctatcga acaactcagg tttatctatc attaatttca aaatatataa aagatgatgt
     1441 acttgtaaaa aacgttaata gtaatcttat acaagatatg ctagattact actattttga
     1501 aaaaaattta tcatataatt atgtaataca tttaaaaact tttacaggaa tgatttttaa
     1561 attcgccaac cgtcgatatg gattaaaata taatcccgta aatggggttt ctttagcaag
     1621 aaaacctaag acaaacgaag aaataaaaaa agaaaaagat ggttatttat caaaagaaga
     1681 agttcaaatg attgctagta aacaaaaaag ttcacatcaa cagtcacgtt attctttaat
     1741 tactaaattt ttgtttttaa ctggactacg ttatggtgaa ttgatatctt tgactgaatc
     1801 tgattatgat ggtaataaaa ttacggtttc tggtacttat gattacgagt taaaaattaa
     1861 aacaacaact aaaaatacag gttcttatag aaccattgaa ttatcaagta atgctaaaga
     1921 aatcatagat caattaattg aagaaaacaa attaattaag aataataaag ataaatacat
     1981 tttcatctct aaaaatggta acccgatatc tattcaagca tataatcaat ctttaagagc
     2041 agtatccaaa gaattaaacc tagataaaaa agtttcttct cacatgttaa ggcattccca
     2101 catttctcta cttacagaat taggaatccc attaaaagcc attatggatc gtgtaggaca
     2161 tgaagattca aaaacaacat taaagattta tactcataca actaaaaata tgcaaaatca
     2221 actagtagaa aaactaggaa aaatagaaat ttaaattgcc ccttttctgc cccttataaa
     2281 aacaaacaac ataaaatata taaaaagaac cccgttataa cgaggttctt ttaatttaac
     2341 tttcaaatac aaattgatta tgataaagtt ccgcataaaa tccattttct gctaataact
     2401 cgtgatgact gccttcttca atgacttccc catcacgcaa gacaacgatt ctatctgcat
     2461 tcaagattgt tttcagccgg tgagcaatca caaagctggt tcttcctttg attgcttcat
     2521 ccattgcttt ttggatcctt gcttctgtta ccgtgtctac attacttgtt gcttcatcca
     2581 aaatcaataa agatggattt gtgatgatcg ttcgagcaat actgatcaac tgcttttgac
     2641 ctgtactgaa taagttattt tcttcggata tctctgtatc ataacctttt tctaacgtca
     2701 tgatgaagtc atgaatattc gcttgttttg ctgcattgat gacttcttca tctgaagcgt
     2761 ccggtttgcc gaatgtgata ttgtctttga tcgttccgga gaacaataca gattcttgca
     2821 aaacgattcc tacatgtcta cgtaatgcat caagatcata atctcggata tctattccat
     2881 cgatgactac tgaaccatca ttgacatcgt aaaaacggtt tagcagattc atgatcgtcg
     2941 ttttccccga tcctgttgga ccaacaagag caaccatttc tcctttgtcc acattgatcg
     3001 agacatcttt caagactagt ttttctggag aataaccaaa atctacgtgc tgcaacgcaa
     3061 gacctttttc aacaccatca aatgctttac agttttctgg tttgacttca tctttttcat
     3121 caaatatttc attcaatcgt cttgcccctg taatcgctaa ttggatcatg ctgtatccag
     3181 atgaaatctg catcagtggt tggtaatatt gttgtgaata ctggacgaat gtcacaacta
     3241 agcctaatgc caccgaacgt tccaaatcac catttaatgc taaccagccg ccgaaaaaga
     3301 taactatcgc tgtattgacc agtgacattc cttgcatcat cggaaatagt agaccagaat
     3361 aagcttgtcc tttgaaggtt gcttcgcgaa cactttgatt atgctctaag aatccttcaa
     3421 tcgtttcttc ttgtaagcca tttgtgatga tgacacgttg tccgctgatt ttttcatcca
     3481 tataaccatt tagtttcccc acttcatctt gctgaagatc cacatatttc cgtgctttgc
     3541 ggataatcaa gacagctatc aaaacggcta ctggagtgga agcaattgta gcccaagcta
     3601 actctacatt ttggcggaac atcatgatca agatcccgac aagtaaaaca gcattcgtga
     3661 tgacttgaag caaagcttgg ttcaaactat tttgaatatt atctaagtca ctggtaaaac
     3721 gacttaaaat ttcaccgtct tgatgagaat caaagaaacg gatcgtcaat ttttccaatt
     3781 tgttgaatag accgatacgc attcggttcg ttgacttccc tactacttgt gtgaacaaaa
     3841 tactatagat aaagttggct gcactagcta gtacataaaa cagcaagagt ttccatatta
     3901 cagaaacaaa tttgctttta tcgtctacgc cttgcatcaa gcctcctacg taattagcta
     3961 attcttgaaa agcttccccg atgtattgag gtgcctttac ttgtagatag gtagcaatga
     4021 tcactgtgat aaagataaag aaaaaggaga gtttgtaccg cttcaaatag tgatagaaaa
     4081 atttacttgc ttttattaga tcagtcatac ttactcctcc gttccttttt gagtttcaaa
     4141 gatttcctga tagacacggt ttccagctac caattcctca tgggttcctt cgccgactaa
     4201 tctgccttca tccaatacta agatgcggtc tgctttgaca acagaagcaa ttttttgtgc
     4261 gatgacaatc gtcgttgttc ccttcaaatc tcgatcgagt gcttctcgga ccagcttttc
     4321 agagcgagca tccagtgcac tcgtactgtc atctaggatc aagatctttg gattaccgat
     4381 aacgccacga gtaatggata aacgttgctt ttgaccacca gaaaagttac tgctacgttc
     4441 ttctattggt gcttcgtaac gatcagataa cttttcgatg aattcttttg cttgagcaat
     4501 agaagaagca tgttccatct ctttttctgt tgcattcttt ttcccttggc gcaagttttg
     4561 agcaatcgtt cctgagaata aaattgcttt ttgcaaaaca aaggaaaccg tttgtcttaa
     4621 actcttttca tttacttgac gaagatctac accacctact tcaactttgc cttctgtcgg
     4681 atcaaataga cgagggatca attgtgctag tgttgacttc ccggcaccag tcgccccgac
     4741 gataccaacc atttctccag gtttgataga aaagctgata tctttaagcg tatctgtatc
     4801 ctcaccagga taacgaaaag aaacatggtc aaaagtgaca gaacccgtca actcttgttc
     4861 tggaacgtct aaataagtaa gatctggttc agcatctaaa atttccgcaa tccgcttcag
     4921 cgatacggct gctcttgaag tcatcatcat catcattccg ccaataatga ttgccatcat
     4981 aatctgcatc aagtagttca tgaatgaagc aattccacca attaacgtag gatcatcttt
     5041 aacaagatcg ctcacaaaaa agatcgcccc aaccacagcc aaattggcag ccagcatgaa
     5101 ggacgggatc atcacagaaa ataatgtacc gactatgatg ttgtgttttg ttaagtcctc
     5161 acttactttg ctgaatgact tcaattggtt ttcttcttgt acaaaagatt tcactacacg
     5221 aatccctagt aagttttctt ttgcaatgct attcacacga tcaatcagtt tttgaatgat
     5281 catgaagtgc ttgcccatcc ttgtaaatga aagtgccgta atcagaaata ccgtaacgat
     5341 aagcgctacg atgatccacc ataattgcgg catcgtgatc atagctaaaa tgaaggcacc
     5401 aataaataag aatggaattc gaaaaagtga ttgcaaagaa atcatcacta aattttggat
     5461 ttgcgtgata tcatttgtta gacgaacaac aagatttcca gcagagaact gttcgatatt
     5521 tccaaatgaa aatgtctgga tctttcgaaa tgcttcttct cggatatctg cacttacgcc
     5581 ttgagctact tttgcagaga aaatcgtgtt cgttacacct gcggctaatc ctagcaaagc
     5641 cagactgatc aggtaaatac caattgtttt catttcatca ttatcttctg taataatagc
     5701 ttccaatact tgctgtagta acttaggttg ccataatgct gctgcaacca tgactgctac
     5761 ggacagcaag gaaataaaaa cagctacttt atattttttc gcatgtcgta agattaattt
     5821 cattcgttga ttacacccct ttgtttaaat tgacaggact aatttaacac tgtaaaatta
     5881 gaccgactaa caatttacca tgtttaaaaa gataatgcaa tacacgaggt tgataaaatg
     5941 gaaataaagc tttctcaagc aatcatcatt caggccgcct ttgatatcct ggctgaaaca
     6001 caagctcttt cgcagctttc catgcgaaac atcgcaaaca gaattcatgt tcaagcacct
     6061 gctttatatt ggtatttcaa aaacaaacaa cagctcttgc aaataatggc tgaaacaatg
     6121 gaagaagaat tagcgcttcc agatccaact ttgccttgga atgaacagtt gctgcagtat
     6181 atggaaaatt actatgacct ttatacacgt ttcccttgtg gggcagaatt agaaatcaat
     6241 actgtgcccg cttttccgag tcgattagaa catttggagc aaatgatcca attacttgtt
     6301 cgtgaaggat tttcagtcac ccaaagtcat caggccattg cctctctcca caatttattg
     6361 attggacagt taatggatca gcagcatgaa cagcagcttc gacaaaaaat tatggacgga
     6421 aatgaatttt tgaaagaaac agttctattt atgcgtaatt atgtagttga aaatcaatta
     6481 gagggattta aagaaagtat gcagtcccat gcagaattga atgaaaaagc actctttcta
     6541 acgagtgcac aaacctatat tgaaggctta tgccaacaaa ataaaagaag agaaaacgag
     6601 cagtaaaaaa gctcctatag agatcactat gtttgtgatc tctataggag ctatcttttt
     6661 aagactcttt ttttattttt cttccattag ccacgccaat gcacgttgaa tccattggtc
     6721 ccagaagatc cagttatgat cgccaggtcc ttgttcaaag gtgaccgcca agccttgttc
     6781 ctgcatagca gataccattt cctgatttgc ctgataaaga aaatcagatt ccccacaagc
     6841 catataaaat cttggaactg gttttccgct ttttactagt tctttcaata agtattgcgg
     6901 atcatttttt gaaccttcga tttgatccaa tggtccaaag atcccttccc aaaaagatgc
     6961 tttacgaacc atcaaaagat catccattcg atttcctaca gaaacagcgc ctgaaagaga
     7021 gattactgct gcgaaatttt ctgattttgc aaggcctagt tttaacgcgc catatccgcc
     7081 catcgataat ccagcggcat atgttttttc tctttttctt gttaattgcg ggaataattc
     7141 atgacaaatc tcaggtagct cttctgcaat gaatgtccaa tacttcatgt cataaactgt
     7201 atctgtgtac cagccaagat cagtagatgg catgatgaca gctaatccgt agtttgctac
     7261 ataacgttca atcgacgtac gtctttccca tacactatga ttgccaccca ttccatgaag
     7321 taaatataaa acaggtatat ccgttgtctc ttgctctgaa ctggtgccaa tcaccttttt
     7381 tgttgtttgc ggtagtatca catttagatt gacttccatt tctaacacat tcgaataaat
     7441 atttgcttga ataaatgcca tattttcgcc ttccttagta agaagttatt tttgaataac
     7501 tttaaatttg aatgattcgg ctacgtacat atcttaaccg aaactactgt tttattttac
     7561 cacttttttc atgaacgaaa agcgttaaga ccaaagcaat gatcacgagt aacaagccaa
     7621 ctagataaac ggaatgtaac ccatcataaa gaatttttct taatggtaac aaaatatccg
     7681 catcaatata tttagccgtt tgtggattca ccagtttatt catgatatct gcatcatttg
     7741 ttaaatttgc tgctgcaagt tctcgtttag ttaccccatt tatcaataaa ccaaaaatag
     7801 aaaccatgac tgtttgacca attgttcgaa ctaacgtatt aaaagaagtt gctacaccaa
     7861 gctggtcacg agatacacta ttttgtgcac tgacagtcga ggtagtcatg gtcacaccta
     7921 agcctatacc gatgaccgtt gatatagcaa aaaaccataa atagttactt ctgaaaggca
     7981 taagatacaa ccaagcgccc ccaatagctg cgattagtag tccaattctc aagacccaat
     8041 gagttttcat ttgagtcatc cacttgcttg caaggaaaga accgaccatc cagactagtg
     8101 ataatggtgc taataccagc ccaccaattg ctgcattcag ccctaataca ccttgcatcc
     8161 acatagggat ataaacatcg attcccatca aaaaaccact agccaatgct gcagccaagt
     8221 tgaccacaac aaatagtgga tttttgaata aatgcatcat gatgacagga tctttagctc
     8281 ttttttcggc aaaaacaaaa atacctaaca ttacgatact acctagcagc aaagcagaaa
     8341 caagcggact cattccttcg tctcctaata attggaaccc taacagcaat gagacaagca
     8401 tggccatcaa actaaaacta ccaaaaatat ccattggttt agactcatgt atgatttttg
     8461 gttcatttaa atatagccaa atcaaaataa ttaatagaat tccgatcggc acattgataa
     8521 aaaagatcca gtgccagctg atcgtttcaa cgatgaatcc tcccgcaagc ggtccaaaaa
     8581 cactggcaat cccccaggct gaactattca aacctaaaac ttttgctcgc ttttcaatag
     8641 aataaagatc gccgatgatc gtcaaagcta ctggcatcat tgccccagca cccatccctt
     8701 gaatcgcacg agcagcaatc aacccaatca tgctttgaga taaaccgcaa aataacgaac
     8761 cgataataaa caataatgtc ccaaaaataa agattggttt ccgtcccact ttatccgcaa
     8821 gttttccgta aatcggagta agcatagcgt ttgtcagcaa ataaatcgaa aatacccagt
     8881 tcatgatttc cattccatgt aatgaaccga caatcgttgg catagcggtt gttacgattg
     8941 taccttcaat agccgtcatg aacgttgcaa tgaaaatact cactgtgacc aaagtaacat
     9001 ttgtttcttt tttcatctta ttcctccctt tcattatgac aggatctaaa aatagataaa
     9061 taattactat ctttttcaag aggttaactg ccataaaaaa agctggaaca accaccactt
     9121 ggcgatcgtt ccagctgaat ataacttatt ttgctaaact tgctttcaac gcatctactt
     9181 tgtccgtatg ttcccaaggc aaatctacat ctgtacgacc aaaatgaccg taagcagctg
     9241 tttgtttgta aatcggacga cgcaaatcaa gcatctcgat gattcctgct ggccgaagat
     9301 cgaagttttc tctcactgca gcaatcagtt cttcttccgg aaccgttcca gtaccgaaag
     9361 tattgattga gatcgaaaca ggttgagcaa caccaatcgc ataagctagc tgtacttctg
     9421 ctttgcgagc aagccctgct gcaacaatgt ttttagcaat ataacgtgca gcatagctgg
     9481 cagaacggtc aacttttgtc gcatctttac cagaaaaagc accgccacca tgacgagcat
     9541 aaccgccata cgtatcaaca atgatttttc ttcctgttaa tccggcatcc ccttgaggac
     9601 caccaatgac aaatcggcca gtcggattga tataatattt cgtttgatca tctaataatt
     9661 cagctggaat aacttccttc actacttttt cgatgacatc atgacgaatt gtttcattat
     9721 ctactgcatc atcatgctgt gttgaaataa cgattgtatc tacgcgttcc ggttgtcctt
     9781 gatcatcata ttcaaccgtt acttgagatt ttgcatctgg acgtaaatac gttaattcat
     9841 ttgatttacg caaatctgcc aagcgtcgta ccagacgatg actcaaagcg attggtaaag
     9901 gcatcaattc tggtgtttca tctacagcaa acccaaacat taaaccttgg tcaccagcac
     9961 caatctcatc taatacgtct tttttatctt catcccggat ctcaagtgct tcatcaactc
    10021 cttgagcaat atcaggagat tgttcatcaa tcgcaaccaa tacagctgct gtatctccat
    10081 caaatccgaa ttttgcacgt gtataaccaa tttcttttat tgtttcccgt acgacttttt
    10141 gaatatctac atatgccgtc gtagatattt ctccaaatac taaaacaaga ccagttgtta
    10201 cagacgtttc acacgctact cgtgctgttg gatcttgttt taaaattgca tccaagattg
    10261 catcactgat ttggtcagca attttatctg gatgtccttc agaaacagat tctgatgtaa
    10321 ataagtgtct ttctaccatt ataatgattc cccctagtta aattcggtta caaggcatct
    10381 ccgtcctgcg gatcctttcg ggaacttgca acgaaccaag tatagcagat aaaacagttt
    10441 tctgctcgta ttttttcatt tttttgctta aaaaatataa aaatatttta cattagaact
    10501 gtccaaaaaa gcttagcaca atcatccata aagataactc tgctaagctt tttaaaatca
    10561 ttctttcaga taaaaagaaa aagatactca aaaagtatct tccatgaccc gtacgggatt
    10621 cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaata
    10681 aaataaatgg gcctaaatgg actcgaacca tcgacctcac gcttatcagg cgtgcgctct
    10741 aaccagctga gctataggcc cataaaaact aaaagcgggt gacgagaatc gaactcgcga
    10801 caacagcttg gaaggctgtg gttttaccac taaactacac ccgcataatg gcgcgggaca
    10861 gaatcgaact gccgacacat ggagcttcaa tccattgctc taccaactga gctaccgagc
    10921 caaacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcatgttaac
    10981 ccctacacca cggaacca
//
LOCUS       DAZKOK010000099        10936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000099 DAZKOK010000000
VERSION     DAZKOK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.099"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG90W_002206"
     CDS             <1..154
                     /locus_tag="ACG90W_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0478538.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG90W_002207"
     CDS             complement(208..486)
                     /locus_tag="ACG90W_002207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478539.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG90W_002208"
     CDS             complement(601..1218)
                     /locus_tag="ACG90W_002208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478540.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG90W_002209"
     CDS             complement(1613..2437)
                     /locus_tag="ACG90W_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0478541.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG90W_002210"
     CDS             2780..3196
                     /locus_tag="ACG90W_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0478542.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG90W_002211"
     CDS             3193..3663
                     /locus_tag="ACG90W_002211"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0478543.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG90W_002212"
     CDS             3677..4450
                     /locus_tag="ACG90W_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0478544.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG90W_002213"
     CDS             4464..5276
                     /locus_tag="ACG90W_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0478545.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG90W_002214"
     CDS             5330..6295
                     /locus_tag="ACG90W_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0478546.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG90W_002215"
     CDS             6431..9154
                     /locus_tag="ACG90W_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0478547.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG90W_002216"
     CDS             9167..9799
                     /locus_tag="ACG90W_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0478548.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG90W_002217"
     CDS             10007..10231
                     /locus_tag="ACG90W_002217"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0478549.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG90W_002218"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG90W_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0478550.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG90W_002219"
     CDS             10556..10825
                     /locus_tag="ACG90W_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0478551.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3625 a         1742 c         2137 g         3432 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct ttttta
//
LOCUS       DAZKOK010000100        10456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000100 DAZKOK010000000
VERSION     DAZKOK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.100"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..1076
                     /locus_tag="ACG90W_002220"
     CDS             180..1076
                     /locus_tag="ACG90W_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0478552.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1226..2167)
                     /locus_tag="ACG90W_002221"
     CDS             complement(1226..2167)
                     /locus_tag="ACG90W_002221"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0478553.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2293..3414
                     /locus_tag="ACG90W_002222"
     CDS             2293..3414
                     /locus_tag="ACG90W_002222"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0478554.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3672..5174
                     /gene="eat(A)"
                     /locus_tag="ACG90W_002223"
     CDS             3672..5174
                     /gene="eat(A)"
                     /locus_tag="ACG90W_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0478555.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5241..5768
                     /locus_tag="ACG90W_002224"
     CDS             5241..5768
                     /locus_tag="ACG90W_002224"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0478556.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5979..7040
                     /locus_tag="ACG90W_002225"
     CDS             5979..7040
                     /locus_tag="ACG90W_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0478557.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7043..7939
                     /gene="murQ"
                     /locus_tag="ACG90W_002226"
     CDS             7043..7939
                     /gene="murQ"
                     /locus_tag="ACG90W_002226"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0478558.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7953..9935
                     /locus_tag="ACG90W_002227"
     CDS             7953..9935
                     /locus_tag="ACG90W_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0478559.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10000..10446)
                     /gene="nrdI"
                     /locus_tag="ACG90W_002228"
     CDS             complement(10000..10446)
                     /gene="nrdI"
                     /locus_tag="ACG90W_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0478560.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3424 a         1848 c         2259 g         2925 t
ORIGIN      
        1 agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa tgatttattt
       61 catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt tttactggct
      121 ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag gcggaaaaga
      181 tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt ttgccttcca
      241 tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc attaaatcag
      301 ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga tttgatatgg
      361 agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt ttttggaatc
      421 acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg gcaatcgatg
      481 agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat aatagttcat
      541 tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta ttttttggtg
      601 caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt ataaaaagag
      661 atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct ggatatctta
      721 cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa ctgttgcggg
      781 agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa aagaaactag
      841 tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat atttttttgc
      901 ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt aagccaatcc
      961 tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt atcactaacg
     1021 attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac aaataaaaaa
     1081 cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat ggcatcatga
     1141 gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca gctgctgatt
     1201 cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga
     1261 ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc
     1321 aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt
     1381 acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt
     1441 acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca aattcagata
     1501 cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca atattacaat
     1561 ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc caaatggaat
     1621 aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact tctacaactt
     1681 tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta
     1741 acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga
     1801 tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca tttagtaaac
     1861 acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata tcatctaatt
     1921 gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg atgatttctt
     1981 ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca gcaatcaacc
     2041 catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg acctcatttc
     2101 cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca gctattgcga
     2161 ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaatgga
     2221 agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt aagaagaaag
     2281 tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag tgcttcttca
     2341 tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag aaaaatcaat
     2401 gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcagggga
     2461 acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat cagctattta
     2521 ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc attggggtta
     2581 gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta tgccttagga
     2641 gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac tttaggagga
     2701 agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc tttagaaggg
     2761 tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg aagaaacgtt
     2821 cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata tacaggtcaa
     2881 caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac gctagctttc
     2941 ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga ggtggattta
     3001 gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt tttgttggga
     3061 gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga aaaaaagatc
     3121 gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac gatccacgga
     3181 aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt
     3241 tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat tgtcttaagt
     3301 attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac attagaaaaa
     3361 acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa gtagttgaca
     3421 aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca
     3481 ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaagga
     3541 agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc tttttgtgtg
     3601 tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct ttttttacga
     3661 ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac gacagccaag
     3721 gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa ctaggactta
     3781 tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac aaactacctt
     3841 atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag acaaaagata
     3901 aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata tgggaaatcg
     3961 aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac
     4021 tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat gacactcatt
     4081 tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa caagtagcgg
     4141 cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg ggatttatcg
     4201 atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt tatcaaggga
     4261 atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg gctcaaaatg
     4321 aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa gccgaatggt
     4381 ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat actgaatcta
     4441 gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa cgatccaaag
     4501 caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta aaagatatcg
     4561 aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga cttttaagcg
     4621 tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat tttacaatcg
     4681 agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca tccattatcc
     4741 attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag ccaaaacatc
     4801 tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg gcggaatttg
     4861 cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag cttgggatgg
     4921 aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg aaaaaagtgg
     4981 aattggctaa atctttatca cagccagctg aactatatac atgggatgaa ccattgaatt
     5041 atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg aaacctgcca
     5101 tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag attatttctc
     5161 ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag gggaactttg
     5221 ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg
     5281 cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga cagatgcagg
     5341 cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt
     5401 cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc agcttgccgc
     5461 tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga ctgccaagca
     5521 aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca aatggggagt
     5581 cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc gaagacgaag
     5641 aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat gcttattttc
     5701 atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa ttggtttctt
     5761 atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg cttttgctta
     5821 gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac ctttagaaat
     5881 gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat atactctagt
     5941 catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt tcaatattta
     6001 tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca tccaatggct
     6061 ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca tatcaaaagc
     6121 gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg gtcgatattt
     6181 caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct ttgaaagcaa
     6241 ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt gccgactggt
     6301 cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat atcgatgaat
     6361 tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat tatccacgtc
     6421 ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc aaaattcaag
     6481 gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca ctatatcaag
     6541 gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct ctagatctat
     6601 tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa aacgttcagg
     6661 agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa cctgtagatg
     6721 aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat gcaagagatg
     6781 tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca agaaatacag
     6841 ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc tatatgggtg
     6901 agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta gtagctaaag
     6961 ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac tatcaattta
     7021 taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa caacggaacg
     7081 cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg ttcaactaat
     7141 gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa gtatcgaacc
     7201 agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt atatgggagc
     7261 cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga cattcggtgt
     7321 agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga ctgtagctgt
     7381 cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc tgaatttgac
     7441 aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg tgatcggtgg
     7501 attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca ataaacaagc
     7561 cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag aatttttgac
     7621 aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata tgatttctac
     7681 tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg tcaaaccaac
     7741 aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag aagctgatta
     7801 cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg ctatcgtcat
     7861 gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag caaacggatt
     7921 tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg actttggaag
     7981 aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa agcattgtcc
     8041 actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat gtctctgaat
     8101 taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa gtcatcatcg
     8161 gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc gtgaaattag
     8221 gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct ggaaaagacc
     8281 gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa aaaccatcta
     8341 aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt cctgcatttg
     8401 tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc acagccggaa
     8461 cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg aaaaacggga
     8521 tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt ggtgcaacac
     8581 caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat ccggaagcac
     8641 cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg atcctaggtg
     8701 tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa gtagtacctg
     8761 atgcggtaga tatcatcgtt acaccaactc tttcattgat tgccatcggt ctgattgaaa
     8821 ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt gggatcaact
     8881 gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta ttcttgccga
     8941 tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg attgatgaaa
     9001 ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa gtgggtgcag
     9061 cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg atcaaaggag
     9121 ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta acattacctc
     9181 taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca gtgatcggaa
     9241 tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg atcccattga
     9301 tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat gctggtggct
     9361 tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa gaattagcga
     9421 ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact tcagctgaaa
     9481 gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat aaagcaagtg
     9541 atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca gaaaacggac
     9601 atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa cacgctattg
     9661 ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac acagttgact
     9721 taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca cctgatacgc
     9781 ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca tccatgatcg
     9841 tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca ggtaaagcaa
     9901 aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat agaaacgaaa
     9961 gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt tttctgctaa
    10021 aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat ctgccaagaa
    10081 aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat tattgaagtt
    10141 tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat gttccagata
    10201 ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat ttccgccgtc
    10261 taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat cactattttc
    10321 atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca ttttttcagc
    10381 atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata tatataaaat
    10441 gttcatggat catcct
//
LOCUS       DAZKOK010000101        10274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000101 DAZKOK010000000
VERSION     DAZKOK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.103"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..233)
                     /gene="acpP"
                     /locus_tag="ACG90W_002229"
     CDS             complement(<1..233)
                     /gene="acpP"
                     /locus_tag="ACG90W_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0478561.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISS"
     gene            complement(355..1356)
                     /gene="plsX"
                     /locus_tag="ACG90W_002230"
     CDS             complement(355..1356)
                     /gene="plsX"
                     /locus_tag="ACG90W_002230"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0478562.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1405..3441)
                     /gene="recG"
                     /locus_tag="ACG90W_002231"
     CDS             complement(1405..3441)
                     /gene="recG"
                     /locus_tag="ACG90W_002231"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0478563.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3558..5240)
                     /locus_tag="ACG90W_002232"
     CDS             complement(3558..5240)
                     /locus_tag="ACG90W_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0478564.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5292..5654)
                     /locus_tag="ACG90W_002233"
     CDS             complement(5292..5654)
                     /locus_tag="ACG90W_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0478565.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5924..6112
                     /gene="rpmB"
                     /locus_tag="ACG90W_002234"
     CDS             5924..6112
                     /gene="rpmB"
                     /locus_tag="ACG90W_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0478566.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6350..9019
                     /locus_tag="ACG90W_002235"
     CDS             6350..9019
                     /locus_tag="ACG90W_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0478567.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9474..10220)
                     /gene="truA"
                     /locus_tag="ACG90W_002236"
     CDS             complement(9474..10220)
                     /gene="truA"
                     /locus_tag="ACG90W_002236"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0478568.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2792 a         2223 c         1850 g         3409 t
ORIGIN      
        1 tggctgctga tataatctac tgcgccaccg actgtttcaa tttgttcagc gtcttcatct
       61 gaaatttccg taccgaattc atcttcaagc tctaaaacga attccataat actaattgaa
      121 tcagctttta aatcatcttt gatgttcaat tcatccgtca ctttatcggt atctatctca
      181 aaatgatttg aaataatttt cgctactttg ttaaatactt cttcgcgagt caactttttc
      241 acctccaaga atcatacact tgtcaattac ctacaccatg gctaatttta ctggcatttt
      301 tcaggaatgt ccaccttttt ttcaatggaa cggatcgctt cagatatcca aatactattc
      361 ttcgcttttt tcaaattgtt cgactaattg tccaactaca tctgtctcaa gcattgtgtg
      421 gatttggcgg atcgtatagc gaacagcatc ggggcctgtt gctccatgag ttttaatgac
      481 tggtgctttc aagccaaaca aaacggcacc gccatgtttg gaataatcca tttctgcttt
      541 caaagaacgt aagccgtctt ttaaaagcat agcgcccatt tttcctttga tcccttcatt
      601 tagaatagca gattttaata gattcatcat attcattgct gttccttcaa tagatttcaa
      661 aacagcgttg cctgtaaatc catctgtgac cactacatca gccacaccat tgagtagctc
      721 acgagcttct acgtttccaa taaaattcaa gcttgtttca ttctgaagga gttcataggc
      781 ttttttcgtc acttcactac cttttgttgc ttctgttccg ttgtttaaga gtgcgacacg
      841 aggacgtccg atacctcgga ctttacgtgc ataaaaagaa cctaaaatac cgtattgcaa
      901 caaatgttct ggtttgtttt ctgcgttagc gccaagatcc agcatatcga aaccggcacc
      961 ttctttccct actactggca aggtagacat caagccaggg cgttcgatat ttttgatacg
     1021 tcctacgatg aacaaccctg ctgctagtag cgcgcctgta ttccctgctg aaaatacagc
     1081 atctgcttca ccgtttttca ctgcttgtgc agcaaggacc attgatgcag tcttttttcg
     1141 tcgaattgct tttactggct catcatcact attgattttt tcgtctgtat ggataatcgt
     1201 gatgtttttt tcatttgtta agtatttctt gatttctgct tcttttccgt aaagtaagaa
     1261 ttctacttct ggaaaatctt gctgcgctaa agccacgcct tctacaattg cttgaggtgc
     1321 gtgatctccg cccattgcat ctacggcaat tttcattgtt tccactcctt tttccgtcat
     1381 cagtataaag tatagcacag tctgctaatc gaaataacga ttatcctgat tttctatttg
     1441 gaggctttgc gctaaaggct gatactcagg aagcatctgc caatccttta tttgccaaag
     1501 ggctgcagcc tcttctctgg ctacctccaa aacatttcca tccgtcacca agtcagcaat
     1561 cgcaaattga ggcagaccag attgacggaa gccaaatact tcaccaggac cacgtaattc
     1621 cagatctttt tcgcttagta caaaaccatt ggtggtctct gtcatgattt tcatccgttc
     1681 cacacctagt tcatttttag gattagcaac taagatacag taggaagcat cacttccgcg
     1741 tccaacacgt ccacgaagtt ggtgcagctg tgccaatcca aatcgatccg catccatgat
     1801 cagcatgacg gtcgcgttcg gcacgtttac ccctacttca attacagtag tcgaaactaa
     1861 tatctgcatc tggttgtctt tgaaggcttc catgattact tctttttcct gattcttcat
     1921 tttaccatgt aacaagccaa cttcatattt tggtgcaaat agttcacgaa gtttttcata
     1981 gatttccact gcatttttta catctaatgc ttctgattct tcaatcagcg gacagatgac
     2041 atacatttga tgtcctcttg ccagttcttt tttcgtccaa tccagtacac tgtccaattg
     2101 aggtgttctt acccagcgtg tttcgatcgg gatacgtcca gctggaagtt catcaatgat
     2161 agatacatcc atctcaccat atgctgtaat agctaatgtt ctaggaatcg gagtcgctgt
     2221 cataaataag acatctggtc gccagccttt ttcacgaagt actttccgct ggttcacgcc
     2281 aaaacggtgc tgctcatccg taataactag acctaaattc tgaaattcga ctccatcttg
     2341 gatcaatgca tgtgtgccga tcacgacgtt aatttcccct tgctctagtt tttcaaggag
     2401 ctcacgtcgt tcttttgctt tcgttgaccc cgtaatcaat gcgatggtta cttcattggg
     2461 atcaaacagc tgttgcaagc tttccatatg ttgttgtgcc aagatttctg taggcaccat
     2521 caacgcacct tggaaacctg ctgtaacggt agcgtataac gcaatcgcgg caaccactgt
     2581 ttttccgcta cctacatctc cctgcaaaag tcgctgcata tgctgcggac tgcgaagatc
     2641 ccgacagatt tcattcgtta cacgtttttg tgcatttgtc agttcaaaag gtagcttttg
     2701 agtaaattgt ttcaattttt ggacatcata aaggatctcc aaaccattcg tttctgcttt
     2761 ttctgctttt ttcaatcctt gaagacgcat ttgaaataaa aagaactctt caaaaacgac
     2821 acgtcgtttg gcttgatggt tttcttcgtg gtcttttgga aaatgcatac ttcttacagc
     2881 agaagctcgg tcaagcagtc gatatttctc cacaaggttg tttggcagat tttcttcaat
     2941 caatgtgccg tattcttgaa accctttttt gattagatca actaatgtcg tttgacgaat
     3001 tgatttgttg acgtgatata tcggcgaaaa gtcatccatt gtttgagaac ctaaaatctt
     3061 catcccgttc aaggctttac gctttgcatc ccatttaccg tatacggcga tttcctccga
     3121 taaaatgact ttttctttta gataaggttg attaaaaaaa gaaacgttga acacatcatg
     3181 ttcttgcatc atacgaaatt gcaatcggct ttttttataa ccaaatcgat tcatcacagg
     3241 aggcgagact acgattcctt tcaaagtaac cttttcttga tcctggatct cgtttagatt
     3301 tctttcctga atatcttcgt agcgaaaagg ataataagtc agtaagtctt caatcgtttg
     3361 gatgcccaag ctggcaagac tagctgctct tttttctccg actcctgcca gcatagtgat
     3421 tggatcatct aaagaacgca taaatttttc ctccctcctt taacaaaaaa aggacttggt
     3481 cagcgatcat acctgatcac taaccgaagg ccttttatga atgaatactg tgttagaaag
     3541 atattctgac acagaaatta ttcagcagaa aatagataag gatagacagg ctgatctcct
     3601 tggtggatct cgatttccaa atcaggatgt ttttctgtta gtttctcagc aaattcattt
     3661 gcttgttcaa ctgttccttc atcacctgcc aagatcgtta cgatctctgt gtcctcattg
     3721 atcatttgtt ctagtgtact taacgctgtt gctgtgatat ctgggttaga gatcaggatt
     3781 tttccatcga tcatgcccaa aaagtctcct tctttgatct ctacaccgtc aattgaggta
     3841 tctctgatcg cgtgagtgac ttgaccgctc acaacgctag acaacatttc tgtcatcgtt
     3901 tctttgtttt cttctaaaga agcttgatca ttgaatgaaa gcatagctgt caatccttgg
     3961 gaaatcgttc ttgaaggaac aactgctact gggatatctg ctacttctgc tgcttgatca
     4021 gctgccatga agatgttctt attgtttggc aagacaatca ctttatctgc gtttacttct
     4081 tgaatcgctt taacaatgtc ttccgtactt ggattcattg tttgtccacc actgataaca
     4141 taattagcgc ctaaactttc aaacagctct tggacgcctt taccagcagc gatcgctacg
     4201 ataccatacg ggatacgtgg tatttctttt ttcgcaaact cgttcacttc ttcatcgtgt
     4261 tccaaaatcg tctcatgctg caagcgcatg ttgtcgactt tgattttgac taatgaaccg
     4321 aatttttgtc cataattcaa gacttctcct gggttttctg tatgcacgtg gactttgatg
     4381 atctcgtcat cattgaccac aagtaaagaa tctcctatac catctaaata gttacggaat
     4441 tcttcgtaat cgaatgtact atcgactgta ggtccttctc cgatttttac catgatttct
     4501 gtacagtaac caaaatggat atcttctgtc gccatttgac cttggacact gcgatgatgt
     4561 tctgcattga ccatatcatc catttctgct ggagaaggtt catatgtttc atccgcttgg
     4621 aattctccat tcaatgcact taaaaagccc tcataaacaa acagcagtcc ttgccctcca
     4681 ctgtctacga caccgacttc ttttaggact ggaagcaagt ctggagtttt agctaaagca
     4741 cgctttgctc cttttacaac agctgtcatt acttcaacga catcatcagt actttttgct
     4801 ttcttttctc cagctttcgc tgcttctcta gctactgtca aaatcgttcc ttctactggt
     4861 ttcattactg ctttatacgc agtttcaact ccatgagtga aggctgcagc aagatcagaa
     4921 gcatttaatg agacaacatc tggaatttgc ttagaaaatc cacgaaacaa ttgagacaaa
     4981 ataacgccgg aatttccgcg cgcacccatc agcaatcctt ttgaaagcat tgctgctaat
     5041 tctcctactt tttctgaggt tgaatctact actgccttag ctccactagt catagataga
     5101 ttcatatttg ttcccgtatc gccatctggc acagggaata catttaatga attgacgtat
     5161 tctgcattta cttgcagacg attcgcacct gcctggacca tttcctggaa ctggcttgca
     5221 cttatttctg ttacactcac ctagataatc ctccttcaga tcgactgctg agttaccagc
     5281 cagtctcctg cctagtctgg cagtacacga acaccttgta caaagacatt aactgaattg
     5341 gcagttacgc ctaataatgc ctctagattg tatttcactt tttcttggac gttgcgggaa
     5401 acttcagaga ttttcgtacc atagctgata atcgtataaa catctacagc aataccatta
     5461 tcttcttggc ggacaactac tcctcttgag taattttctt tacgcaaaat ctcagtaata
     5521 ttatctttaa tttgattttt gctggccatt cctacaacac cgaacacatc tgtcgcggcg
     5581 ccaccaacaa ctgttgcaat tacatcattg gtaatttcaa tggttccagc tgacgtttga
     5641 atttttacag ccatgaatga gcctccttaa cgggtaaaaa actcccattt ttcttctatc
     5701 atattttatc atagcgcaac ttgaaataaa aggcagttac ccaaagtact caaaaacctt
     5761 gcaaaatttc aggtataacc ttgtttaatt tacaattatt cttctcaatt gtcaattgta
     5821 tctatgaaaa tgtacttgca aattcccatt ctatatgata tgttagtctg gtatgagctg
     5881 aattattcta ttgctccaaa tcaaggcaaa ggaggaaatt accatggcaa aagtttgtta
     5941 ctttacaggt cgcaagacaa aaagtggaaa caaccgttca cacgctatga actcaacaaa
     6001 acgtactgtt aaacctaact tacaaaaagt tcgcgttatg gtcgacggaa aacctaaaaa
     6061 agtttgggtg tcaactcgtg ctttgaaatc aggaaaagtt gaacgcgttt aattgaatga
     6121 tacttggacc gttcagatag tgaacggttt tttatttaat aaaaattttt aagggtggga
     6181 cttttgattc ctaccctttt tttgcgttta tttttgttat acgaacaaat tatgaagtta
     6241 tttattctaa attttatttt tttgtgaaaa ctagactctt tttaagcaga gaaaaacaag
     6301 gatatgataa actgaataca acttcataga aatttggtgg aaaataaata tgaaaaaaaa
     6361 tttaaagtca ttctttagag aaaacggacg ctacgcagca gctagtttcc tgattccgtt
     6421 tttgatttta gcacttgttt atctaagtat cggaatctat cctggaagca gcagaagtat
     6481 tttggcaagt gatgcctttt cacagttttc taattttcat gccagcttcc gaaatgttct
     6541 tcttggaaag caaagtattt tttatacatg gaatgcttct ctaggattga attatttatc
     6601 tttaatttct tactatctag gcgggatctt cacgccactg gtgttctttt ttccaaacca
     6661 gatgatgcca gatgcactgt atgttctgac tttgcttaag gtcggctgtg ccggattggc
     6721 tttttggttt cttgcacgta cgttcaagat tcctcgctgg gggcatgttg cacttagcgt
     6781 cagttacgca ttgatctcat ttatcactgc acattcggag atcatcatgt ggctggatgc
     6841 atttttttat cttccattag tgattttggg gattcatcgt gttatggatc ttaaacggcc
     6901 gaaactttta tttatcagtt atttactctt gtttttatcc agtttttata tgggttttat
     6961 gattggcgta ttttctttct tatattttat cgcccgtcta ttgacaaact ggtcagaata
     7021 taaaaaaacg attgttcctt atggtatcac ttctttactg gcaggcggcg cttcaatgat
     7081 cattgtattg ccagcagtgc tggatcttcg gtcaaatggc gaggcactga gtcagatcac
     7141 tacgttgaaa acagaagcga cttcttatct ggatctaatc atgaaaaata tgattggtgt
     7201 ttatgacacg acaaaatacg gatcgattcc gttcgtttac gcaggtctgc taccattagt
     7261 cctctgtctg ttttattttg ccagtcggaa agtaccgttg aaaaataaat ttttgtacgg
     7321 cagtcttttc gccatactta tcgcaagttt ttatcttgtt ccattgaatt tgttctggca
     7381 tggtatgcat gcaccgaata tgtttttatt ccgttatgcc tatctgttct ctttcttagt
     7441 gctcctgctt gcaggaaaag gatgggaaaa gatagaacaa gacgatcgtg ggctgttgtt
     7501 aggaatcatt ttggtcgtag ctgctctctt tgcggtggca tggggcatca aacctgaagg
     7561 aagctacacc tatgtgacga tgacttcctt tgtattgaca gtggttttct tagggattta
     7621 tgcttttgct gtgggatttt atcagcataa aaaactttca ttcaatcatc tgagtatatt
     7681 gttattattg atcatgtctg ctgaagcact ggtcaatacg aacagtatga tccatggcat
     7741 tcttgatgat tggaattatg cttcccgcag tttatatact gatccttatc ctgacttgaa
     7801 agctcttgta aatgaaacaa aaaaagattc cgactcgttt taccgcttgg aaaatctgga
     7861 tccagtatct gcaaacgatt cgatcaacta tggttacagt ggtatcagtt tgttttcttc
     7921 tataagaaat cgtaattctt cctcttacct ggataaatta ggtttccgtt caagagggac
     7981 aaatttgaat atccgttata acaataatac tttgctgatg gatgggttta caggaatcaa
     8041 atataacatt tctaaaaacg aaagcacgtt cagtaaatat ggcttccgcc aagctggtca
     8101 aagcggcgac tatacactgt atcagaatgt caatgcattg ccactagcat ttactgcacc
     8161 gttgtccgtt aatgatgttg agcagccagt gacagataat ttgaccagtc agacaaactt
     8221 gatcaataca ttgtctggtc taaatgaaca gtattttaca ttctatacac ctactttgaa
     8281 gaatcagttg aatacgacga tcacaaagac aacgactggc gtcacttact cagaaacagc
     8341 agataaccag ccaaaagagc tcacttggga agtccatgtg cctgcaaata cacaagctta
     8401 cttaagtttg ttcccaacca attttgcgca attagaaagt tctagtgcga cgatcactgt
     8461 caatggtaca tcgcgaaaaa cgcaaatcaa catttctggt caatactatg atcttggcta
     8521 ttatcctcaa gagacaacag tgacatttac tgcaagtttt tatgggacga aagaaataag
     8581 tttcatggag cctaaagtcg ttggtctaga tgttgtagcc tatcaagccg caatgaatca
     8641 gatcaaagaa aaaggtgtcg aaatggaagc cactggcaga aaagcttctg ggactgtaaa
     8701 taccgctgaa gattccatgt tggtgacaac tattccttat gacaaaggat ggaaagctaa
     8761 aattgatggg aaaccagtca aggtcagcaa tttcaaagac gcattcttga tgataaaagt
     8821 tccagcaggt aaacatactg tcactttcag ttacttgcct gaaggattta cgataggtgc
     8881 aatccttttt gtcctatgta ttgcattgtt tattctttat gtctggtacc ttgaccgcaa
     8941 tcgccccttt tatggcacga ttgatccagc aatagaagaa caaccacgaa acacacacag
     9001 aagaagacgt agaaaataaa gaaacgatgg aacctgttca gactgtccgc accaagggca
     9061 atcaaaacaa cgttccatcg ttttttggtt acagaaaaaa taggtaacaa aaagatgatt
     9121 cacttatcaa cgcctcgtcc acaagtcacg atattcatca aagatgagaa gctgtttttt
     9181 gaaattgctc tttgtgactc agagttaaat gacaggctca cgacctcttt cttaaacggt
     9241 gttcaatcag ctgaccttcg gtattagctc aacaaacggc aaaatatgag gagctatttt
     9301 cccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacgacctct tccttaaacg
     9361 gtgttccaga agtagcttct tcgaaaataa gccgaaattt tgcaaaattt tgagaagtaa
     9421 ttttcgtaaa taccttctta tttcttgaag ctgaccactt ctatcataac ctcttattca
     9481 tatttcactt catacaaata aagtccttca gggtgtgctg taggtcctgc tgcattacga
     9541 tcttttttcg caataatatc tgggatacta tcttcaggca atcggccatt gccaattttc
     9601 aaaagcgttc ctactaagat ccgtatcatc ttatacaaga agccatttcc cctaaatgta
     9661 aacaacaatt cgtcttttgt ttcatttacc tccatcttag cctcgtagat cgtacgtatt
     9721 ttatcctcta tcgagcttcc tgatgcacaa aaagaggtga aatcgtgagt ccctaataaa
     9781 tcaggaagcg cccgctgaat tttttccaaa tcaagtgggt aaggaaaata acttgcataa
     9841 tggcgtcgaa aaggactgcg tggtttccct atatccactc gaaattgata cgtcttttct
     9901 ttgaccaaat atcttgcgtg gaagtcttct gaaacaattt ctacttgtct tacagcaata
     9961 tcctctggtg actgggtatc tagcgcgaaa cgcattcgtt ctaacggacg ctcctctgga
    10021 taatcaaaat ggattacttg cccgatcgca tgtactccgg catctgtcct tcccgaacca
    10081 aagaccgtga tttcttttcc atttgccatt ttttgtaatg ttttttctac ttcttcttga
    10141 acagtacgcc catttggctg tttttggaat ccattaaaat ttgttccgtc gtacgcaatg
    10201 attgctttgt aacgtaccat gtttttctct cctttgttcc taaaaggtta tctggaataa
    10261 tgatttttct ataa
//
LOCUS       DAZKOK010000102         9950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000102 DAZKOK010000000
VERSION     DAZKOK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.104"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1557)
                     /locus_tag="ACG90W_002237"
     CDS             complement(46..1557)
                     /locus_tag="ACG90W_002237"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0478569.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG90W_002238"
     CDS             complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG90W_002238"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0478570.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="ACG90W_002239"
     CDS             complement(3384..4460)
                     /locus_tag="ACG90W_002239"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0478571.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="ACG90W_002240"
     CDS             4656..6401
                     /locus_tag="ACG90W_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0478572.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="ACG90W_002241"
     CDS             complement(6681..7118)
                     /locus_tag="ACG90W_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0478573.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="ACG90W_002242"
     CDS             complement(7102..8475)
                     /locus_tag="ACG90W_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478574.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="ACG90W_002243"
     CDS             complement(8797..9285)
                     /locus_tag="ACG90W_002243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478575.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="ACG90W_002244"
     CDS             complement(9307..9909)
                     /locus_tag="ACG90W_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0478576.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2913 a         2119 c         1798 g         3120 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc
//
LOCUS       DAZKOK010000103         9836 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000103 DAZKOK010000000
VERSION     DAZKOK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.105"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..241)
                     /locus_tag="ACG90W_002245"
     CDS             complement(35..241)
                     /locus_tag="ACG90W_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478577.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(257..871)
                     /locus_tag="ACG90W_002246"
     CDS             complement(257..871)
                     /locus_tag="ACG90W_002246"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0478578.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1018..1701)
                     /locus_tag="ACG90W_002247"
     CDS             complement(1018..1701)
                     /locus_tag="ACG90W_002247"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0478579.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1810..2268)
                     /locus_tag="ACG90W_002248"
     CDS             complement(1810..2268)
                     /locus_tag="ACG90W_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0478580.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2352..4115)
                     /locus_tag="ACG90W_002249"
     CDS             complement(2352..4115)
                     /locus_tag="ACG90W_002249"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0478581.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4336..5796)
                     /locus_tag="ACG90W_002250"
     CDS             complement(4336..5796)
                     /locus_tag="ACG90W_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478582.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6004..6930)
                     /gene="glsA"
                     /locus_tag="ACG90W_002251"
     CDS             complement(6004..6930)
                     /gene="glsA"
                     /locus_tag="ACG90W_002251"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0478583.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6953..8380)
                     /locus_tag="ACG90W_002252"
     CDS             complement(6953..8380)
                     /locus_tag="ACG90W_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0478584.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8411..9625)
                     /locus_tag="ACG90W_002253"
     CDS             complement(8411..9625)
                     /locus_tag="ACG90W_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0478585.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2913 a         2043 c         1628 g         3252 t
ORIGIN      
        1 ttttatatgt tttttcactt ttcggaataa atatttattt gttaaagtaa ttaaacaatc
       61 cgctaatcgt ttgatctggc atttctattt ccttaccatc taaagtaaca gtagtaacga
      121 caaaatggtc ttgcaatggc ggaacttcta agtactgccc ttgcagaaaa tcattcactg
      181 tttcatattc ttttttcatt tcttttgaat ctgtcgatgg tttatagctt atttttaaca
      241 taactagtgc tcctttttat tcgggacgtg caatcgtgaa aatttctcct attgctgcat
      301 aatcatttcc tgccaaacgg cttacagcat tcaattttct tggatcgatc cttcctgtct
      361 tatcatcata gactgtttca tttaaatgat atttctgtac ttctagcaaa aataaatcag
      421 ctgttacttt atttttattc cgtatctcga catgttcata taaggatgtt tcaaaacgaa
      481 cttttgcttc tgtcaatgct ggtactgaaa tgattactga atcagttaaa gtcaagtcag
      541 tcaggtacag ttcgctttcc tcattaggca gattggctgc tgttttattt acttcttcta
      601 cgttgtcctc atcaaggata tggataacag cttctcttgt ctcaagaata ttctttgctg
      661 tgtcttttac tgcatcttgt tttctctgga tcgccaagga taagatgggt cgattcgaac
      721 tgacgatatt gaagtaactg aagggtgcga tattcactat atcttttgct gatttagtcg
      781 ttactactgc tactggtcta gggatgactg aaccaatcag caacttgtaa ttttcacgtt
      841 ctgtcaattc tgaaggactc cgttcaaaca tggtttcgcc ttctttctat aaaattcgtt
      901 aaaaagcaga tcttgaagac aaaatgaaaa gtaatgcttt tttcatcttc ttagaatctg
      961 ctttaaacta tctatcaatt tttctttttt gtcaagaatc tgtgccgtgc gtgtttttta
     1021 gttatttgct ttacttgcgg cttcgacata tcttttagtg atcaattgcg gacgggtgat
     1081 aatagatcca actacgactg aaaaagcacc taattcatat gctttctcta cttgttcagg
     1141 agagtcagta tgtccttcca taattactgg gatatcgact tgttctatga tttgattcaa
     1201 taaatcaaaa tcaggtgtct gcgtctttcg tgaatattct gtatagcctg ctaaagtcgt
     1261 agacaaaaga tcaaaaccat cttcttgcgc ttgaagcgct tcctcaaaag tcgaaatatc
     1321 tgccatagct aaccgaccgt gttcgtgaat catttggaca agttcagcag ttgttttttc
     1381 tgtcggacgt ttacgagaag ttgcatccag tgcaatgatt tctgcttgcg tctccaaaag
     1441 ttcttccact tctttttctg tcgcagtaat aaacacttct gacccatcgt aatctctttt
     1501 gataatccct attaccggta aatcaacgat tcgcttgatt tcattgatat cttctgtcgt
     1561 atttgctcga ataccaactg ctccgccttc ttttgcagcc aaagccattc tccccataat
     1621 aaaagaactg tgtagcggct cgttttccaa agcttgacaa gaaacgacga ctccgccttt
     1681 gatttgttcc agtgtatcca caatatctcc tccagtaatg ttatataaaa atacagcttc
     1741 atataaaaat gtaacccttt tctattcagg agacaataat gagctatttc tttcagcaga
     1801 cctttcttac taaaagaaaa ttacttcaaa ttcttcgtat acgaccagct tctcttctgt
     1861 aaattctatg ttcaacgctt ctttataagc atgtttaaaa aactcagcat ggactttccg
     1921 ccaataagct aatgtccgat ccccttctcc ttctttccaa gcatgctctt tcgtgacttc
     1981 actaaaaggc aagatggtca catttatatt tttgatgata caaactggtt cattttttcc
     2041 attcaggacc acgtctaatt gaccaacttt tggtaaaggc tcattctctt gtttatacag
     2101 atcgtaacca cttgatgtac cagtctttat cttttttaca actagttcgc tcaactcgtc
     2161 tgccattttt tctgtatccc caaatgaaaa gaaacctaat ttttctgatt gataactagg
     2221 atatttttct tggaactttt tgacatattc ttctacttgc tgatacataa catccacccc
     2281 tttgcttttt ataatgaaaa aaccatccag atactagtat aaacaattat ctggatggtg
     2341 agcataatga attattgaat attcaagaca cgcagactat cttcaacgat ttgttttggt
     2401 gttaaatgat aacgttcata aagctgccct acaggaacgc tatccgtgaa ttctttctct
     2461 gcaccaaaat ttaatacgtt cattgaagaa tcaccataat aacgactgat attttcgcca
     2521 aatcctcctg acaaactacc atcttctaat gtgatgacta attcgtgtgt ttcttttaat
     2581 ttatctaatg tttctacgtc attttctgtg atcgttcctg gattgatcaa cgttgcttcg
     2641 attccttttt ttctaaaagc ttttactgtt tcttttccta attcaagaaa accgcctaaa
     2701 gctaaaattg ctacttttcc tccattattt actgtttgat aggctggaat actataatca
     2761 tttgcgatag ctgtaccatg ttctacacca tgtgtaggaa ttcggattgc tacaggctgc
     2821 gctgtttgtt cgaacgccca atctagcata gagatcatat cctcttttgt tgctggagca
     2881 agatatttga tgttcgggat acttgataag taaggaatgt caaatgttcc ttggtgtgtt
     2941 cggtcactag ctgagatcgt tccgccaccg actaaaataa tcactggatt ctcgttgatc
     3001 gctaaatcat gtgataattg atcatatgcc cgttgcaaga aagtacttga ttggaagacg
     3061 actggacgta ctccctgaga agccaaagca gaagcgaaag tgatagagtg ctgttcagcg
     3121 atccccacgt caaaataatg atctggatgt ttttcttgga atgttttcag tccaaaggcg
     3181 cctggtatcg ctgcattgac tgcggcaata ggagctgtac ttgtttgcat tttcttatcc
     3241 aaataatcca tgatgatcat attaaatgtt tctccagaag ttacttgctt tgcctccccg
     3301 gtggacagat caaatggtac atgccaatga tatttcattt tattatcgat tgccggctga
     3361 taaccgcgac ctttttccgt atggacgtga agtacaatcg gatgatcaat atcttttact
     3421 tcttcaaata aacggatcac cgtttccaga tcgtttccat tatccacgta acgataatca
     3481 aatcccattg ctttaaatag gttattgctg cttttaccgt tggtttgacg aagctcagct
     3541 aagtttttat ataaactgcc atgattctca tcaatagaca tctcattatc gtttactaat
     3601 acgattagat tggattgtaa aacaccacca ttgttcaatc cttcaaaagc taagccacca
     3661 cttagggagc cgtcaccgat aaatgctacc acatttcctg attctttctt cagatctctc
     3721 gctttagcta aacctgttgc taagctgata gatgtagatg tgtgccccac tgtaaaatag
     3781 tcatgtttac tttcatgttg gttggaatat ccgctcactt cacggtaatg tcctttttca
     3841 aaaccatttt ttcgaccagt taaaagctta tgaccataag actgatggga aacatcccaa
     3901 acgattttat cctttggtga atcaaataca taatgaaacg caatcgttaa ttctactaca
     3961 cctagattcg gaccaacgtg cccacctaca tcgctgtcct tttgcaagat tttttggcgc
     4021 atctcttctg ccaactgttt catttcctca acagataatt ttttgacatc tgcggggcta
     4081 ttgattgcat ccaatacatg taatactgta ctcataatac attctctcct ccgtactatc
     4141 ttcttttaat gaaaatagat ttgttacact ttatctattc tactacttag agttgactct
     4201 aagtcaatag cattttaaaa aaatttcaga gaaagcttgt tatataagta gatcttcgca
     4261 cgaatcagga ctcaaataaa aaccttctac agacaattaa aaagctttgt agaaggtctc
     4321 ctaattaagg tctcatcaag aaatatgttt ttttaatagg tgaagttcct gagcaatatg
     4381 atacaaatgg atgtttgcac cttttaatga gattggatag atctttgtct tttcagtttc
     4441 taatccattt tgatagctag cagcatcgca caaaaccact ctttttttcg cacatagaca
     4501 ttcatcaggc aattcttcta atgaacgaaa taaaatagga tttatttcag cgatgccttc
     4561 ttccgcatac aaatgaaatt gggttcgata aaaaaattgt ttgactgaac tttctgaaac
     4621 aacaaaaatt tctatttcct gtctccccgc ttgttcactg ttgatcaaag aaagactttt
     4681 gacgaatttt cgaatgaaat tttcattcca tcttagttgg atttgatttt tctttttcca
     4741 ctcttccaat atttctacaa tttttccgta cagaagttgt tctttttctg atagaagata
     4801 gatcctttcc ggtaaaaata cttgtccatc tgcccacaac gtacggacaa attgtaaaaa
     4861 ggattgcttg aaaataaaat tttctgcatc gtcgatttca gcgatactaa taatctgtaa
     4921 aatcaattca ttcagtgata ggtcattgag aacaaaattt ttataaacaa cttcgaaatc
     4981 ttttcttaac agctccatgt tctcattggt ataatttcta ctgttgaaca aagaaaaaat
     5041 ataatagaca tcttcttctt gtaagtctag aattcctttt tgctcttcca atccttgaca
     5101 gactaattga aataacggct tcgtttctgc gcttttctta tcttcttctt tcagtccaag
     5161 gggaaatgcc tttcttccga ttgcgatctc tattcctcgt atgataaagg acttttcatc
     5221 ttccgaatat cttcttttaa aaaaatgagt ctctacataa tcaatcaact cgttacaagc
     5281 ttcttctaca tacggatttt tattttcata gccttcccaa ttgacataac gtactaaagc
     5341 tagcaagatg ttccgcgagt caaattctgg gataatgatt tctttatttt tcaaataatc
     5401 attttcttta aaaaaatcca atacgatttt tttgattgta tatactttgc tcaaagaaat
     5461 aaattcaaga tcagccagtt ctgtactcgt gaatatacca ttaaaataat aacttaaaaa
     5521 ctttaaaaaa taagattgac cgtacagaaa ctgtgtgagc tcagaaagag caataccatg
     5581 tttgaatacg agtttatact tatgcttctc tcttacttgt tcttttatac aatattctag
     5641 acactcggct agactttcta gatcgtgaag gatcgttggt gtagtcaccc ttagagaaat
     5701 agctgtttgt tcgatgtcta tttcttggaa ttcaaataat tgttcacaca gatacacttt
     5761 tctaaatata tttcgctcga tgtaattttc cagcattcct gatacacgcc tccagttttt
     5821 cttatgattt tatttaatca tatctgtaaa tcgtattttt gtttcctttt tatttagaaa
     5881 agccctttca aaaaccaata aaaaaagaaa ctgggacaaa agtcaaacaa gctcacaatt
     5941 tcagcctcat tgtaggtagc tttgagccgt ttggactttt accccagcct ctttcaagaa
     6001 tggttattca aagatgtcag cgtctagttc tttgactaca tctttcaaca attggatacc
     6061 agctgcgctg ttaccaaatg gatctagtgc cgggctgaag acacctatgc cgtaacgatt
     6121 tggcacagct gccatcaacc cgccgcctac accgcttttt gcaggtacac caacatggac
     6181 ggaaaactct cctgattcat catacaaacc agcagttgtc ataattgatt tgacgattgt
     6241 agcactttct tcagtaatga tttgtttgcc attccaagga gcaattcctt tatttgccag
     6301 cacagcagct aattttgcta tgcctgtagc ggtgacattg agggagcatt gtctgaaata
     6361 agcatcgagt atctccggta cgtctccttc gatcatttga ttacctttca tatagtaagc
     6421 aagagagcga ttgatatcac ctgttcttga ttcagaatga tagatttctt cattcaatgt
     6481 gatattagga tcatcacaga tctctttcat aaatgacaag attcgctcaa atttttcatc
     6541 tgcatcttta ccggcaatca acgaggttgt tgcaatcgct cctgcattaa caaaaggatt
     6601 cattggatgt ttgcgatgat tgatttccat attcatgata gaattgaagg caaaaccggt
     6661 tggttccgtg ttgattgttt gaaagacttt ttctattccg ttgtctttga ttgctaataa
     6721 caatacggga acttttgaca tgctttcgat ggcaaagcgc acatcggcgt ctcctccctc
     6781 aatcagttgt tttgtatcca aatcataaat cgccatacct aattgtttgg gatccacttc
     6841 agctaatgct ggaatgtaat ccgccacttg tcctgtttga taatagtgga aattttttcc
     6901 aatcaattgt tctaattctt ttacttccat ttgaataacc agctttcatt ttttattttt
     6961 ctgcatgctg gaatttatga cggcatgcgt agatcgtaaa tggaataact aagacgataa
     7021 gataagaaac aatcagaatc gtttcataaa tcccatgctg tccagctgcg atactagcag
     7081 gtggaacaaa tgagatacaa aaagcaaaaa gtgaagtcaa aagacctaaa caagcaacga
     7141 ttacttttac aacttttccg cctggaacct gataggctct ttcaagactg tcatctttaa
     7201 aaaccagctt caaatagccc gcgaaaaaca taaagtacaa catcaggtag attacaacag
     7261 tcaatgacat cgctgcaaga aatgaaagat tattcccccc gccgcttaat gtaaagacgg
     7321 ctgcccagat tgttgcaacg atcccatttg cgatcatcaa atgagcagga acaccgtgtg
     7381 tgttttcttt tgcaaaatat tcaggaagta atccttcttc tgctgctacg tgaagtgctt
     7441 ttgaaggaga ggttacccaa gaagcaattt ctccgatgac acctaaacaa acaagaatcg
     7501 caagaatttt cgcaatccat gcaagatttg gatcaatgtg tcttaatagg aattctaacg
     7561 attgaatcac ccctgtatta agagacaaac catgttgagg tactgtagta gcgacggtca
     7621 atccgccaaa agtatctaaa atgattgcca gaataaccaa tacaaacatt gctaatggat
     7681 aatccttttt agggttttcc atttcatttg catgactggc agatgtttct acgcccatat
     7741 atgctaaaat aaaggaaaca aaaacaacca gcgtaggcaa tttcgaaaaa tcaggaatca
     7801 atgttttcat agacagtatc gtttgtagtg gagcgcctga agcgacataa tggatcccta
     7861 agccaaacaa gactaatgct ggcaacaaga taccagctac aaaaccgatc cgagcaatca
     7921 atgaagtgta tttggttcct tttaactgtg agagtaaaat agcccagaaa ataatcaata
     7981 ggattgccaa tttcagatat ttattgtttt gtatagcacc aatacccgtt gcataggata
     8041 atgcaccagt taagaaatac agcatggtca agtagccaac tgttatctca aaccattgga
     8101 aaaagattgc ggcaaaaccg aatcgttttc ccaatgtgcg actgacccaa gtatagacac
     8161 cgcctttttc ccatcctttc accgttgcca tctctgctgc gcacaatgcc acagggataa
     8221 accatagcaa accgcctaat aacaagaaaa atactaagct gaagcctgag gtcgcgaatg
     8281 tgggatattc atataatgaa acgaccattg agatggtcat agccaaaaag ccaaagaacg
     8341 ttaatttttt tgcaggaatt ttcgttttgc tttgttgcat gaatcttcct cttttctatt
     8401 tgaatttgtt tcaattgagt ttgttgccaa agaagaaaaa gtccaatcct tcttcttgtt
     8461 cttcttttgt agtagtgatc gttgtaaagt gcatcgccga ttttaaaatc actgttgtca
     8521 gcacataggc atacacggca gtaatgatga caccaatcac ttgaatccct aagaagtacc
     8581 agctgcttgg attcgttagt aaatggctgt tagatgcaaa gatccctatc agaatcgtac
     8641 ctgtgaatcc gcccatgcca tgaacacccc acacatcaag tgcatcatcc cattgtttct
     8701 ttttacgaaa atcaacagca gcatgacaga cgatacccgc aatgatccca atcagcatgg
     8761 cactttttgc ttctacataa ccggcacaag gcgtaatggc tgccaatcca gcaacggatc
     8821 ctgttagtac gtcgacaaaa tcaaagccat ttccttttac ttttgcaatg atcagccaag
     8881 taatcatcgc aaatgcaaga gcgatgaatg ttgaaccaaa tgcattgact gcttgttcat
     8941 ctgctcgaag tgcaccaccg gaattaaaac cgaaccagcc aaaccaaagc agtcctgtac
     9001 cgatagcagc tgccataaga ctgctatgct gacttctttc tcctaaagga atctttcttt
     9061 gaccaagtgt gaggatacat gctaaagagc cgaatccagc tgtcgtgtgg atgactgctc
     9121 caccagcgaa atctctaaat cccatctgat ccaaaaatcc gccaccccat atccagtggc
     9181 aaacagggaa ataaatcagc agattccaaa aaataacaag tataatatag ccttttaaat
     9241 tcaatctccc tgcaaaagct cctgtcatca aaggaacggt aatcacgata aacatcagtt
     9301 ggtaaagaaa gaacattaaa aatggaatcg ttgcaccatg aaacatgttg gggaagcttg
     9361 taacatgtct catcaaaaag aaatctgccg ggtttccaat aacaccgccg atatcccgtc
     9421 caaaggccaa tccgaatcca ccaaataccc acataatcgt tgttatccca acggagagaa
     9481 aagattgaga catgatcgtt agtatattat tcttccgtgc taatccgccg taaaaaaaag
     9541 ctagaccagg agtcatcagg caaaccattg ctgtacataa aatcatgaat gctgtatctc
     9601 ctgtattgat aggtaatgta ttcatttttt tcgcctcctt tctacgatta cgagtataaa
     9661 agaaggtgta gttgtttttt tcttctgttt ttttgaatta atacttgcat ttataaaaga
     9721 cagacaatta ataaaagctt gatgtgttgt gctctttttc gtttttcaca caaataaaga
     9781 caaaaaataa acggtcttct ttttatagaa aaccgtttat tttcgtcttt ttttat
//
LOCUS       DAZKOK010000104         9811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000104 DAZKOK010000000
VERSION     DAZKOK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.106"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG90W_002254"
     CDS             complement(34..2154)
                     /locus_tag="ACG90W_002254"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0478586.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG90W_002255"
     CDS             2384..3562
                     /locus_tag="ACG90W_002255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0478587.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG90W_002256"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG90W_002256"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0478588.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG90W_002257"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG90W_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0478589.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG90W_002258"
     CDS             complement(4915..6168)
                     /locus_tag="ACG90W_002258"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0478590.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG90W_002259"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG90W_002259"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0478591.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG90W_002260"
     CDS             7746..9092
                     /locus_tag="ACG90W_002260"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0478592.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG90W_002261"
     CDS             complement(9400..9786)
                     /locus_tag="ACG90W_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0478593.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAZKOK010000105         9731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000105 DAZKOK010000000
VERSION     DAZKOK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.107"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG90W_002262"
     CDS             34..657
                     /locus_tag="ACG90W_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478594.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG90W_002263"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG90W_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0478595.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG90W_002264"
     CDS             2153..3034
                     /locus_tag="ACG90W_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478596.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG90W_002265"
     CDS             3037..3807
                     /locus_tag="ACG90W_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478597.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG90W_002266"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG90W_002266"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0478598.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG90W_002267"
     CDS             5118..6281
                     /locus_tag="ACG90W_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0478599.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG90W_002268"
     CDS             6398..6808
                     /locus_tag="ACG90W_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0478600.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG90W_002269"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG90W_002269"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0478601.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="ACG90W_002270"
     CDS             9381..9665
                     /locus_tag="ACG90W_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478602.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3377 a         1648 c         2089 g         2617 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa a
//
LOCUS       DAZKOK010000106         9644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000106 DAZKOK010000000
VERSION     DAZKOK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.108"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG90W_002271"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG90W_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0478603.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACG90W_002272"
     CDS             1995..4661
                     /locus_tag="ACG90W_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0478604.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACG90W_002273"
     CDS             5025..5777
                     /locus_tag="ACG90W_002273"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0478605.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACG90W_002274"
     CDS             complement(5799..6953)
                     /locus_tag="ACG90W_002274"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0478606.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACG90W_002275"
     CDS             7138..9579
                     /locus_tag="ACG90W_002275"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0478607.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3111 a         1736 c         2066 g         2731 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tttt
//
LOCUS       DAZKOK010000107         9593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000107 DAZKOK010000000
VERSION     DAZKOK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.109"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG90W_002276"
     CDS             complement(<1..641)
                     /locus_tag="ACG90W_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0478608.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG90W_002277"
     CDS             complement(643..1407)
                     /locus_tag="ACG90W_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0478609.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG90W_002278"
     CDS             complement(1415..2092)
                     /locus_tag="ACG90W_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0478610.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG90W_002279"
     CDS             complement(2129..3514)
                     /locus_tag="ACG90W_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0478611.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG90W_002280"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG90W_002280"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG90W_002281"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG90W_002281"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0478612.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG90W_002282"
     CDS             4453..5310
                     /locus_tag="ACG90W_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478613.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG90W_002283"
     CDS             complement(5391..5726)
                     /locus_tag="ACG90W_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478614.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG90W_002284"
     CDS             complement(5793..7163)
                     /locus_tag="ACG90W_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0478615.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG90W_002285"
     CDS             complement(7241..7777)
                     /locus_tag="ACG90W_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0478616.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACG90W_002286"
     CDS             8086..9567
                     /locus_tag="ACG90W_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478617.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DAZKOK010000108         9530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000108 DAZKOK010000000
VERSION     DAZKOK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.110"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..987
                     /gene="gnd"
                     /locus_tag="ACG90W_002287"
     CDS             85..987
                     /gene="gnd"
                     /locus_tag="ACG90W_002287"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0478618.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            989..1990
                     /locus_tag="ACG90W_002288"
     CDS             989..1990
                     /locus_tag="ACG90W_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0478619.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1987..2271
                     /locus_tag="ACG90W_002289"
     CDS             1987..2271
                     /locus_tag="ACG90W_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0478620.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2304..3101
                     /locus_tag="ACG90W_002290"
     CDS             2304..3101
                     /locus_tag="ACG90W_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0478621.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3115..3948
                     /locus_tag="ACG90W_002291"
     CDS             3115..3948
                     /locus_tag="ACG90W_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0478622.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3966..4823
                     /locus_tag="ACG90W_002292"
     CDS             3966..4823
                     /locus_tag="ACG90W_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0478623.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4845..5354
                     /locus_tag="ACG90W_002293"
     CDS             4845..5354
                     /locus_tag="ACG90W_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0478624.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5391..5792
                     /locus_tag="ACG90W_002294"
     CDS             5391..5792
                     /locus_tag="ACG90W_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478625.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5904..6257
                     /locus_tag="ACG90W_002295"
     CDS             5904..6257
                     /locus_tag="ACG90W_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478626.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6291..6734
                     /locus_tag="ACG90W_002296"
     CDS             6291..6734
                     /locus_tag="ACG90W_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0478627.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6751..7836
                     /gene="uxuA"
                     /locus_tag="ACG90W_002297"
     CDS             6751..7836
                     /gene="uxuA"
                     /locus_tag="ACG90W_002297"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0478628.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7829..8542
                     /locus_tag="ACG90W_002298"
     CDS             7829..8542
                     /locus_tag="ACG90W_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0478629.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8749..8898)
                     /locus_tag="ACG90W_002299"
     CDS             complement(8749..8898)
                     /locus_tag="ACG90W_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478630.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9294..9494
                     /locus_tag="ACG90W_002300"
     CDS             9294..9494
                     /locus_tag="ACG90W_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0478631.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3210 a         1496 c         2110 g         2714 t
ORIGIN      
        1 aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag
       61 ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga
      121 ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca
      181 agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa
      241 tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata
      301 accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa
      361 ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa
      421 atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc
      481 ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta
      541 gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg
      601 atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta
      661 caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg
      721 gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta
      781 agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg
      841 ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca
      901 caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga
      961 catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc
     1021 cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt
     1081 tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg
     1141 attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa
     1201 agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc
     1261 agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt
     1321 agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc
     1381 aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa
     1441 caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg
     1501 taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga
     1561 aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg
     1621 tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca
     1681 gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc
     1741 gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga
     1801 agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca
     1861 tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt
     1921 cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat
     1981 aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat
     2041 ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa
     2101 agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt
     2161 ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct
     2221 gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg
     2281 gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg
     2341 gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata
     2401 aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta
     2461 taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag
     2521 atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg
     2581 aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc
     2641 attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc
     2701 tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa
     2761 gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc
     2821 gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc
     2881 gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc
     2941 tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg gctgaattta
     3001 ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac
     3061 agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa
     3121 acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc
     3181 tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc
     3241 gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg
     3301 atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca
     3361 gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt
     3421 gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact
     3481 tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga
     3541 tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt
     3601 gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc
     3661 gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact
     3721 gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt
     3781 gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc
     3841 gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac
     3901 aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat
     3961 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
     4021 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
     4081 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
     4141 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
     4201 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
     4261 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
     4321 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
     4381 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
     4441 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
     4501 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
     4561 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
     4621 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
     4681 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
     4741 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
     4801 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
     4861 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
     4921 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     4981 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     5041 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     5101 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     5161 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     5221 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     5281 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     5341 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     5401 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     5461 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     5521 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     5581 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     5641 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     5701 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     5761 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     5821 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     5881 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     5941 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     6001 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     6061 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     6121 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     6181 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     6241 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     6301 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     6361 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     6421 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     6481 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     6541 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     6601 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     6661 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     6721 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     6781 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     6841 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     6901 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     6961 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     7021 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     7081 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     7141 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     7201 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     7261 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     7321 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     7381 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     7441 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     7501 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     7561 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     7621 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     7681 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     7741 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     7801 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     7861 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     7921 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     7981 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     8041 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     8101 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     8161 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     8221 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     8281 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     8341 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     8401 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     8461 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     8521 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     8581 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     8641 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     8701 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     8761 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     8821 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     8881 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     8941 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     9001 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     9061 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaaattta
     9121 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     9181 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     9241 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     9301 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     9361 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     9421 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     9481 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa
//
LOCUS       DAZKOK010000109         9432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000109 DAZKOK010000000
VERSION     DAZKOK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.111"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACG90W_002301"
     CDS             <1..680
                     /locus_tag="ACG90W_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0478632.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACG90W_002302"
     CDS             800..1000
                     /locus_tag="ACG90W_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0478633.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACG90W_002303"
     CDS             1076..1273
                     /locus_tag="ACG90W_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478634.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACG90W_002304"
     CDS             complement(1570..3117)
                     /locus_tag="ACG90W_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0478635.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACG90W_002305"
     CDS             complement(3201..4004)
                     /locus_tag="ACG90W_002305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0478636.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG90W_002306"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG90W_002306"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0478637.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG90W_002307"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG90W_002307"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0478638.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG90W_002308"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG90W_002308"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478639.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACG90W_002309"
     CDS             complement(6389..7318)
                     /locus_tag="ACG90W_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0478640.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACG90W_002310"
     CDS             7513..8196
                     /locus_tag="ACG90W_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0478641.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACG90W_002311"
     CDS             8357..9400
                     /locus_tag="ACG90W_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0478642.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAZKOK010000110         9350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000110 DAZKOK010000000
VERSION     DAZKOK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.112"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(423..920)
                     /locus_tag="ACG90W_002312"
     CDS             complement(423..920)
                     /locus_tag="ACG90W_002312"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0478643.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(960..1769)
                     /locus_tag="ACG90W_002313"
     CDS             complement(960..1769)
                     /locus_tag="ACG90W_002313"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0478644.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2103..2960
                     /locus_tag="ACG90W_002314"
     CDS             2103..2960
                     /locus_tag="ACG90W_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0478645.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2953..3855
                     /locus_tag="ACG90W_002315"
     CDS             2953..3855
                     /locus_tag="ACG90W_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0478646.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3888..4238
                     /locus_tag="ACG90W_002316"
     CDS             3888..4238
                     /locus_tag="ACG90W_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0478647.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4287..5210
                     /locus_tag="ACG90W_002317"
     CDS             4287..5210
                     /locus_tag="ACG90W_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0478648.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5288..6181
                     /locus_tag="ACG90W_002318"
     CDS             5288..6181
                     /locus_tag="ACG90W_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0478649.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6219..6782
                     /gene="amaP"
                     /locus_tag="ACG90W_002319"
     CDS             6219..6782
                     /gene="amaP"
                     /locus_tag="ACG90W_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0478650.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6796..6984
                     /locus_tag="ACG90W_002320"
     CDS             6796..6984
                     /locus_tag="ACG90W_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0478651.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6997..7899
                     /locus_tag="ACG90W_002321"
     CDS             6997..7899
                     /locus_tag="ACG90W_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0478652.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7931..8173
                     /locus_tag="ACG90W_002322"
     CDS             7931..8173
                     /locus_tag="ACG90W_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0478653.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8429..8800)
                     /locus_tag="ACG90W_002323"
     CDS             complement(8429..8800)
                     /locus_tag="ACG90W_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478654.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3283 a         1605 c         1803 g         2659 t
ORIGIN      
        1 taaccgtccg gtcgtaggtt cgagtcctac aaggtacata agaggttgtg aaaaagctgc
       61 cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt tacttggctg
      121 ttcttattac tgcagatgca agcttttgaa taccgtttaa ggaagaggtt gtgaaatcag
      181 cgttttgacc tgagtattag cttaacaaac ggaaaaatag ttcctcatat tttgccgttt
      241 gttaagctaa taccgagggt cagctgattg aacaccattt aagaaagagg tcgtgagcct
      301 gccgtttcac tctgtgtcat aaaaaagcca atctactttg atagaaaaag cacgtaggac
      361 taaaacagtt ctgcgtgctt tttttattgt gtgttaaata tcgtaaatga agatagatat
      421 ttttatgatt cgacgataga gacgttgaaa tctttcaaca gctctaaagc ggattctccg
      481 tgttttctac tggagactaa cattggatca acaaaaaagt ctgcttcagg aaaagcactc
      541 tgcaggccta tcagattaga gagtacacac aagtttgttt caatcccgac tacttcgatc
      601 tctttttgtt cttctttgct ttcaaataaa gtttcctgga tttcagctaa agtttcaggt
      661 ggaatcgtaa agtaatactt tttaatcact cgttcatttt cttgtggaga aagaagaggg
      721 atcaattgca aatcttctcg ttcttcttct ttgtccttca gttcaactgg tatatcccgt
      781 gtgaagagaa tatattcatt attttggcgt gctttagcta atctttgagc aatacgttct
      841 gccaattgat ctgactctgg aatataaaat tcgctgtttg gatcaagaat atgattttgc
      901 atatcaataa taactaacat tttctttcct cctacattca tatcgtcatt ttattctttt
      961 taatagaagt tttttaaaat ataagcaaca ccagcttctt cattcgtcag tgttacttgg
     1021 tttgcaattt cttttacttg tcgctgtgca ttccccatcg caatactcaa cccgctgtaa
     1081 ttcaacatag caatgtcgtt ttcttgatcg ccaaaagcca tgatgtcact cgattcgatt
     1141 ccaaggtaat ccgccagaaa aacaagtgaa tgagctttag tgatacctgt aggcgtgatt
     1201 tctaagaaat gcggatcaga gaatactgat tggataccat ctgcttgcag atctctttgt
     1261 agcttctgta gtttttcttc actcttatct gttaatgcta atttaagata gctaagagaa
     1321 ggatcttcca acatatttaa aatttgatca aaagaatggt tctgtaaatt gaaatgttca
     1381 taataagggt catctttgac tagatttgat tcatcgaatg tcagctgatc atcttgggtt
     1441 tctcggctag caatgatgga tattttcgct tcttgagcat agaagaaggc ttttctcagt
     1501 gtatcgctat ctatttttga acgtctgaga actttcgcca tttttccatc cttcatttca
     1561 gcaatcagtg aaccattgga agaaatcaaa tagccaccga ttcctaattc ggaaaaaata
     1621 tctttagcag aaatgctgtt ccttccggta gcgacggtta cgattccgcc attttcatca
     1681 aatttccgaa gtgcttggat attttcttct ggtatctttc catttcgatc aagcaaggta
     1741 ccgtctaaat caatcgctgc tagtttcatt ttatcttcct actttctttt tacatatcta
     1801 ttttcctgtt accttaagat taaatgagaa tttaaaaaaa tgaaactaac ttgctttgtc
     1861 attatgaatt ttttcaaaaa acattcttaa gaaaaaggtg gctatatata gtagtttttg
     1921 gtcaatacct ttgaataaaa agccatgaaa aacgaatagt tagcgcttac cgatgcgttt
     1981 tgtgataaaa aaattttaaa atattcaatg aaaacaaagc atatattttg ctttaaaacc
     2041 aaatgaggaa tattctagat gtagaacagg ttagaaaaag attagaaaaa ggagtgattt
     2101 tgatggagaa gagatacggc gtagttcctg tggtaactgc ttcagacgaa aattatgctc
     2161 cttacttgag cgtgatgatc gcaactgcat tagaaaattg caataaaaca agacgcataa
     2221 aattttatgt gattgacgat ggtctatcag aatatagtaa agaaggattg gaagaaacgg
     2281 taaataaata ttcaagtaat gcaagtatcc aattcctgac agtggaaaaa gatatctacg
     2341 aagacttcct agtcagtgac catatcacaa ctaccgctta cttaagaatt tctttaccaa
     2401 acttattagc taaagaggat tataagaaag tcctttactt agattcagat gtgttagtac
     2461 tagacgatat cgtaaaacta tatgatgagc cgttaaatgg aaaaacaatc ggcgctatta
     2521 tcgatccagg ccaagtgaaa gctttggaaa gattaggaat cgattccgat gacttgtact
     2581 tcaattcagg agtgatggtg attgacattg atcaatggaa taaaaaagaa attacagaaa
     2641 aaactatcca ttacctcagt gaaaatggcg atcgaatcat ctatcacgac caagatgctt
     2701 tgaatgctgt actttatgaa gattgggaac aacttcatcc aaaatggaac atgcagactt
     2761 cgctgatttt tgaacgtcat ccagcaccaa atgaaaaata tgaacgtcag tataaagaag
     2821 gaaatgaaaa accttcgatc gttcacttta caggacatga taaaccttgg aatacgttga
     2881 aagatcatcc gtataccaat ttatacttaa aaaaattagc acacagtact ctaacgaaag
     2941 taggcgagat caatgaataa aaaagaaata gcagtagtcg caagttgtaa cacaaaattc
     3001 gtaccacatt tagctgcatt atttgtctca gtattagata attgcaaccc gtctaaattt
     3061 gttcggttct atgtgattga cgatgatatc gattttgaaa gtaaacagct tttacgcttc
     3121 tcagtgaaaa acgcacgaat gaactcagat gtggaattct tgaaaatcaa taaagaattc
     3181 tttacaaatg tggtaatcag cgatcgtatt ccagaaacag cttattatcg tattgcgatt
     3241 ccggaattgt tccgcggcac agaagttgaa cgtatcttat atatggattg cgacatgatc
     3301 gctttacaag atatctctaa attatggcgt cttgacttcg gtgattcaat cgtagcagct
     3361 gttgaagacg ctgggttcca tcaacgactg gaaaaaatga aaatttcagc taaatcaatg
     3421 cgttatttca actcaggttt gatgctgatc aatgtgaaaa aatggttaga tgaaaatatt
     3481 acgcaaaaag tattagattt catcgaacat aatccagaaa aattacgatt ccatgaccaa
     3541 gatgcattaa atgctatctt acatgaccgt tggttaccac ttcatccgcg ttggaatgca
     3601 caaggctata tcatggcaaa agcaaaaaaa catccaacag cagcaggtga acgtgaatac
     3661 gaagaaactc gcaacaatcc ttacattatc catttttcag gacatgtgaa accgtggagc
     3721 aaggattttg aagggccaac gaagaaatat tacgaaaaat atgcagggat gacggctttc
     3781 cgttgtgtag ctaaattccc taaatatcca aaatatgcaa aagttcaacc aaaaacagat
     3841 gtagttgtta aataatagaa gacaacaact gaagaggggg agtaggaatg gaaattacaa
     3901 tgtctaaaaa tgcagtagag acgttaattg aaaaggttgg ggaaaataca aaaatcgcac
     3961 tcgcgcttat caatgattcc gatccatttc taagggacaa aggtgctttt gcaaaaggca
     4021 gcttttttca aatcattcct tttgtttcgg aatttggaga atatgcgact aaaatcgagc
     4081 atccattatt ggacatctat acatctaaat tagagcaaaa ttatttcggt aaacgattga
     4141 acatggactt caacaaacag cttgacagct tttcattaga aaatgaaatc gcagtactcg
     4201 attataatat caaattaaag aactgcttct tcagctaata gtttcgagaa agtccaaaaa
     4261 ggaggtatac attgagatac tactttgtgg atgaagaaaa gaatgtaata gaaacagatg
     4321 aaaaaaaata taattggctc gtacttgata gtgcaaagcc cgaagagatt gagcaagcaa
     4381 tcgaagaatt cgagcttcct gatgatattt tcgtaggaac cgaatatccg gaagaagttt
     4441 ctcggatgga gcgcctttat gagacgaaac tagagcatcc attttctttg gtagtgatta
     4501 atctagatca gtcagataat cgtatcgaaa aaaaattaac gccaatatct tttgtacttt
     4561 ccgataatct cttgattact tgtatcgata gaaaaaccga atttgtggat cggctgctga
     4621 caaagcatgg aaatcattta aactcgtttg aaaagatcat cacgtataca ttattagata
     4681 tttacaccca ttacgtgaaa gaactaagag agatgaaaaa gcgaattgac gctttggata
     4741 aagcagctcg taaaacaacg gaaaatgaag aactgtataa acaggcagat cttgaaagag
     4801 acatcgtata tatcgatcat accttaagag atcaacggga aacaatggat caattatgga
     4861 attctgaaac tttcatcaaa cgtttagacg atgaacagct actctatgat gtccatttga
     4921 gacagcgaca aacagaaaaa atgatcgaga tctatcgaga tttattagag agtattggtg
     4981 atctattcaa cgggatgatg gacaataatc taaatcatat catgaaatat ctagattcag
     5041 ctgcattaat catttccgtt ccggcgttgt tcgcagggat ttggggaatg aatacaggag
     5101 gattaccggg taaaagttct gctctaggct ttatactcgt agtctttggc tctttgatag
     5161 cagcagtagt tttaggttac catttatacc ggaaagatta tacaaattaa agaatctatt
     5221 taaaacaggt atcaaacaaa gaaatttgtt ttggcaatac aatttaaaaa ctaggaggaa
     5281 tacagcaatg acagaaccaa aattaatcga cccgcgaaac ttgtaccata cggagaaatt
     5341 tcctaaacaa gatcaggata caccagcttt acagcagaat atgattcctg ttcctgattg
     5401 tggagaagaa tcctatgaag gcaataatca actagaaaac cgccgtgtac taatcactgg
     5461 tggagattca gggatcggtc gtgcagcagc gattgcattt gctagagaag gggcagatat
     5521 cgctcttcaa ttcttcccag gagaagaaaa agatgcagag gaagtagcaa aatacgtgaa
     5581 agttgcagga cgtaagattg tcttactacc ttttgatctt cgtgacgaac aagcacctaa
     5641 agaaatcata gataaagccg tgagtgaact tggcgggcta gatacgatgg tactaaacgc
     5701 aggacagcaa atttcttgtc agtctattgc tgagttacca cttgagcaag tcaaagacac
     5761 atttatgata aacatcatcg ctatgtttgc tttagtgaaa gaagctgttc cgcatatccc
     5821 agcaggagga gcaatcgtaa caacaacttc tgttcaagca ttcaatccaa gcgaacattt
     5881 attagattac gcagcaacaa aagcttcaat cgctaacttt acgatcggtt tagcaaaaca
     5941 gcttgcacct aaaggaatcc gtgtaaatgg tgtagcacct ggtccaatct ggacaccatt
     6001 acaactagac gaaggacaac cttctgattc gatccctgaa tttggtcagc aaagtttact
     6061 agaacgtgca ggtcagccag gagaattagc accagtttat gttttcttag catcgaataa
     6121 agcaagttat gttacagccc aaatttacgg agtaactggt ggagaagcga tcaacttata
     6181 agaagttgat cgtttcaccc ctatgaggag gtgcgaaaat gaataaaggt gtaaaaacga
     6241 tcggagtgat cggaacatta cttttgctat ctgtgctttt gtttgcgata ctattaagtg
     6301 caacctatcc attgcctttt cagctagaga agtttcgatt cttttccatc actaattcat
     6361 acgtgcaaca atatgtattt tggcttgccg ttgcatttgc tattttgaca attatcacgt
     6421 tacttgtttt cctgttttat ccaaaaacaa tcggaaactt tctactgaaa catggtgatg
     6481 gggagttgac gttggataaa aaagcaattg aaggaatggt tcgttctcac ttgcatgaag
     6541 atgaattcat ccattcacca aaagtaaatg tcaaagcaac gaaaaacaag atcgccgtca
     6601 atgtaaaagg ggagttgaaa cgaacttcag cactcattgg taaaacagga acattgatga
     6661 aagaaatcga aaatgaagta caacacatat tgggaacaaa agagccagtg aaagtatcgg
     6721 tgaaatacac aggctttgaa gaaccagcta aacgaagcaa tgaccgcaaa cgagtagaat
     6781 agggggggac caaaaatgaa agaactattt tcgacataca agctccctat catctttggg
     6841 gtacttggat tgatattagc cgttttatgg atcaccgtgg gctttttcaa gaccttattg
     6901 atcttgatca tggcagcgat cggcgtcgct atcggttttt atctgaaaca aacgaaaatc
     6961 ttagatcact attttaaatc ttaggaggaa acatccatga ataacaataa caaaaataat
     7021 aacaataaaa tgaacaacca aaaaccagtg tctgcacatc cagacccaac gacaccaaac
     7081 caatcaaatg gtgtgaacga tgaaaagaac aaaacagcac cagtatctgc acatccagat
     7141 ccagtagtcc ctaacgttca acaaggagta gaagagaaga aacatgatgc agaaaagcat
     7201 gcacctcatc atccaggaac tccagaatct aaagttccag aacaacatga tgcaatcgat
     7261 ccttctaaac ctgtggaaga gcaaaaacat ccttctgacc atgcgaaagc acaagaaaaa
     7321 cttagttctg accacacaaa cggacaagga aaacttggtt ctgatcatac aaataaccaa
     7381 ggaaagcatg attctgaaca ggaagattca atgcgcggtg agctgaatta tgaagataaa
     7441 gttgtgcaaa aaatcatcgg aatcgcaatt gaacaagtag acggtttatt atctgcaaac
     7501 ggtggtttct tctctaatgt ggcaggaaaa cttgtgaata cagataatgt aacggctggg
     7561 atcgaaacag aagtcggtaa aaaacaagta gcagttgata tggatgtcat tgtggaatat
     7621 ggcaaagata tcgaaaaaat ctttgaagaa atgcaagaag taatcggcaa agaagtaaaa
     7681 aatatgacac atttagaagt gatcgaagtc aacgctaacg tagtggacat caaaacaaaa
     7741 gaagaatttg aaaaagatca agaaacggtg caagacaaag taactgacgc agcgaaaaaa
     7801 accggtgaat ttgcttcgaa tcaaacagat aaagtaaaat cagcagctgg aagcggcgca
     7861 caaaaagtaa aagaaaatac agaaccacgg gttcagtaat aaggaagtaa aacattagga
     7921 ggaacgaaac atgggattta tttggtcact tatcgtcgga ggaattatcg gagcaattgc
     7981 aggagcaatc acgaataaag gttcttcgat ggggattatt gcaaatgtcg tagcaggttt
     8041 agttggatca gcacttggtc aagcattatt aggttcttgg ggaccaagta ttgcaggaat
     8101 ggcaatcgtg ccatcattga ttggtgccat cattttagta ctaatcgtat cattctttgc
     8161 tcgaaaactg taaaaagact tttaaaaaga ggtcgtgaaa aagctgttct tcatcaagta
     8221 ataagaacag ccaaacaaaa atagcttctc atatttttca tttggttggg catattatcg
     8281 aagatgcaag cttttgaaca ctgtttatac ggaataagag gctgtgacaa gacttttgtc
     8341 acagcctctt atttatgcgg gcaaattccc aattagattt ttaaaagttt ttccttaaat
     8401 gtgtctttat tgatacagag aagcttcttt aataaaacaa tgcctcccta aattttcttg
     8461 agataggaaa ggtgaacata caaaataata cgaccacttt gaaaacccag atgatccaca
     8521 aatggatcgt atgatactga tgaagtaaaa agcaatggat cagaaactca aagagattga
     8581 aaaagacaag gaagaaataa tatctctttt ttactcttgt ctgttttagc caataatagc
     8641 tcgtaaagta atctagacaa acaaatagaa gtaaacaaag tttcgtaaga gcagcaatcg
     8701 tatcatgttg ctctgctaga cgattgacga gcaggaaaaa tgttggtaat ggaatataga
     8761 tcacgagtaa tgaaagaaaa aagtaaacaa taaatggcat actattcctc ctaattgtgt
     8821 gagttcgccc tcattttaaa ataacgatat ttgtttgtga agctttttcg tgtcgtaaca
     8881 aatgttatct aatgtatttt taaataaata atcattatta tcaaaataat gttaaaaata
     8941 aaaaagaatt gtccatcact gttattcaaa tcattttatc aaaactacat agtaaatgtt
     9001 aaaaagcaac atgacataat atatagaagg ctttacagta tatatgggag cttactaaaa
     9061 aagaatggag taagagttaa cacaatgggc cattagctga agttttaagc acttcaaggt
     9121 atgaaacaga atggcctgta atactacatg attatttaga ctgctcaaac tgctatcagt
     9181 ctggtggaat gaataaagta ttatactaaa agcagatcca actacaacgt aacaggaaac
     9241 cagttgtatc aggaaaagtg atagtacatg gagaagaaaa ggcccccttt gtatgagtca
     9301 gtacatatct tatttataga taaaacggta ttcaatcgaa ataaaaaatc
//
LOCUS       DAZKOK010000111         9285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000111 DAZKOK010000000
VERSION     DAZKOK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.113"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..684)
                     /locus_tag="ACG90W_002324"
     CDS             complement(16..684)
                     /locus_tag="ACG90W_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0478655.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            818..1105
                     /locus_tag="ACG90W_002325"
     CDS             818..1105
                     /locus_tag="ACG90W_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478656.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1521..3059
                     /locus_tag="ACG90W_002326"
     CDS             1521..3059
                     /locus_tag="ACG90W_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0478657.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3056..4021
                     /locus_tag="ACG90W_002327"
     CDS             3056..4021
                     /locus_tag="ACG90W_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478658.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4008..5330
                     /locus_tag="ACG90W_002328"
     CDS             4008..5330
                     /locus_tag="ACG90W_002328"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0478659.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5393..5497
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5571..6314
                     /locus_tag="ACG90W_002329"
     CDS             5571..6314
                     /locus_tag="ACG90W_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478660.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6329..7321
                     /locus_tag="ACG90W_002330"
     CDS             6329..7321
                     /locus_tag="ACG90W_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0478661.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7333..8070
                     /locus_tag="ACG90W_002331"
     CDS             7333..8070
                     /locus_tag="ACG90W_002331"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478662.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8060..8782
                     /locus_tag="ACG90W_002332"
     CDS             8060..8782
                     /locus_tag="ACG90W_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0478663.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8866..>9285)
                     /locus_tag="ACG90W_002333"
     CDS             complement(8866..>9285)
                     /locus_tag="ACG90W_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0478664.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3039 a         1603 c         1964 g         2679 t
ORIGIN      
        1 agtgaaaaat aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc
       61 tgtagtctgt tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt
      121 attcaatgta taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgagatc
      181 caaataaagc aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat
      241 cttaatatga aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc
      301 gccgaatcga aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt
      361 gatcagtaga ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa
      421 aactagcgca aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa
      481 tgagataagc aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa
      541 aagattatgc aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat
      601 attgattcct gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc
      661 tgtaaataaa tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact
      721 tctttattat actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg
      781 gctgtttctt tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac
      841 aattcttaca aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca
      901 aaaactggtg cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt
      961 tgaacacggc aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg
     1021 tgagaaagct gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga
     1081 gatagaaaat aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa
     1141 aacaaccatt ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga
     1201 acaggccaat aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta
     1261 ttaaaataaa agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat
     1321 agatttttat ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg
     1381 cttggattct ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct
     1441 gtaaggttaa ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga
     1501 ataacagaaa ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg
     1561 agttgctgga agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag
     1621 cactgattgc tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct
     1681 tattgcagat cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta
     1741 tcccatttgt agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc
     1801 ttccgatttt ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag
     1861 cagcggtagc atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg
     1921 cattacctgt cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta
     1981 ctttggcagc attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc
     2041 gaaatacacc tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat
     2101 tcagcggact gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag
     2161 cagcagtggt gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg
     2221 aaaccatcac gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata
     2281 aagaattgat tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga
     2341 cgagtttttt gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag
     2401 gtactgtcct agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa
     2461 aagaaaaaac atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat
     2521 taaaaccaat ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg
     2581 aacatcaact taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat
     2641 tcacactgga atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct
     2701 tgaatttttc aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg
     2761 gagatgtcaa tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag
     2821 tgactctaga agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa
     2881 aagattttgc taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa
     2941 taacagagga tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga
     3001 cagaagtagc acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac
     3061 taaattaatc gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga
     3121 cctaaatctg gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg
     3181 aaaaacaact actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta
     3241 cttcaaagga aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata
     3301 tgtgctgcag caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat
     3361 ccctgaaatg ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca
     3421 aaaagttggc ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg
     3481 agaacagcaa cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat
     3541 ggacgagcca tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt
     3601 gtccttacac gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc
     3661 aatcaagcta ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac
     3721 accagagcag ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc
     3781 gaaacagacg aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga
     3841 aataccagaa aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc
     3901 gcttctttcg gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct
     3961 gacaagtaaa gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg
     4021 atgcaatcat catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag
     4081 caggtaaaac agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg
     4141 gatgtgatcc taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat
     4201 tgagtggaaa aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca
     4261 aacgaacatt tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa
     4321 tcgatcatct ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag
     4381 aagtcttcca tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag
     4441 tggagggaaa ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta
     4501 aagaaatcat ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg
     4561 ctgcagggag caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg
     4621 cgtctttggt agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg
     4681 gtgttgaaac ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga
     4741 cggaattagt agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt
     4801 tagcacttga gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc
     4861 tgcaaacgag caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa
     4921 aattgacacc agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa
     4981 caagcgaacc tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg
     5041 ctgaagtagg agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc
     5101 ttaccagttg gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct
     5161 ttgaccagca aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga
     5221 tcgatgattt gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata
     5281 tcatggggta tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat
     5341 aactaaagcg cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt
     5401 tctggggatg tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc
     5461 agttaatact ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc
     5521 ccatttcaag cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa
     5581 atctttatcc agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag
     5641 tttggaaaat acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg
     5701 cagatgcaca agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt
     5761 tggtaatagc tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt
     5821 tagaaatgtc tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag
     5881 ggccattact taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct
     5941 tgataagcta ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag
     6001 agcaaatcat ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata
     6061 aaataccagc gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg
     6121 tcagcggagc agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga
     6181 tacgcgtaaa aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc
     6241 tattgagttt attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc
     6301 tttatatttt ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact
     6361 gttgctttta tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga
     6421 tttgaaaaaa gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta
     6481 cctagcaaaa gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc
     6541 aggagataat tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag
     6601 ttatcaagaa gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat
     6661 tgctacagtg atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca
     6721 ttcaccaaaa gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc
     6781 tgtcttaaaa gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg
     6841 aagcaatgga gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga
     6901 aggttgggat atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa
     6961 agaattagat gaacggttag attactatac tccggtaatt attacgaatg atcaactgat
     7021 caaaagtgat ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga
     7081 agctattaag gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga
     7141 tcggacgttt ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga
     7201 gaattgggga ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa
     7261 cggattgatc aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata
     7321 ggagtgatag atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa
     7381 atattagaag acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca
     7441 agcggagccg gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct
     7501 ggaacgatca aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg
     7561 ccgcaagaag atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa
     7621 aaaatcaatc atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg
     7681 ttaaacggct atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg
     7741 gccttgttac gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga
     7801 gcgttagatg ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa
     7861 gcaaagcgga ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac
     7921 aggattcttg tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa
     7981 aaacgacggt cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag
     8041 ttattggcag gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag
     8101 tttagcactc cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga
     8161 agaatggaag gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat
     8221 ccatccttgg aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc
     8281 aaggatcatc ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca
     8341 aaaaaaagta tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact
     8401 gcacacaaaa ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta
     8461 tttgatccat tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta
     8521 tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt
     8581 cccaatcgac cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg
     8641 ggggattttt tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc
     8701 tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg
     8761 tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt
     8821 ggctttcttt gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt
     8881 tgcttcccag aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc
     8941 tgctactctt tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg
     9001 gtaacgtccg atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc
     9061 gatccattct tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg
     9121 agcaggttgt gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac
     9181 aacaggttgt tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc
     9241 ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAZKOK010000112         9253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000112 DAZKOK010000000
VERSION     DAZKOK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.114"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG90W_002334"
     CDS             <1..435
                     /locus_tag="ACG90W_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0478665.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG90W_002335"
     tRNA            complement(759..842)
                     /locus_tag="ACG90W_002335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG90W_002336"
     CDS             1370..1870
                     /locus_tag="ACG90W_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478666.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG90W_002337"
     CDS             complement(2060..2404)
                     /locus_tag="ACG90W_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0478667.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG90W_002338"
     CDS             complement(2558..3478)
                     /locus_tag="ACG90W_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0478668.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG90W_002339"
     CDS             3665..4156
                     /locus_tag="ACG90W_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0478669.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG90W_002340"
     CDS             complement(4180..6555)
                     /locus_tag="ACG90W_002340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0478670.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG90W_002341"
     CDS             6881..7507
                     /locus_tag="ACG90W_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0478671.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG90W_002342"
     CDS             7530..8234
                     /locus_tag="ACG90W_002342"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0478672.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..>9253
                     /locus_tag="ACG90W_002343"
     CDS             8426..>9253
                     /locus_tag="ACG90W_002343"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0478673.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     "
BASE COUNT         2816 a         1785 c         1648 g         3004 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gca
//
LOCUS       DAZKOK010000113         9022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000113 DAZKOK010000000
VERSION     DAZKOK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.115"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..366)
                     /locus_tag="ACG90W_002344"
     CDS             complement(46..366)
                     /locus_tag="ACG90W_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0478674.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="ACG90W_002345"
     CDS             complement(514..2748)
                     /locus_tag="ACG90W_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0478675.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="ACG90W_002346"
     CDS             3227..3493
                     /locus_tag="ACG90W_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0478676.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG90W_002347"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="ACG90W_002347"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0478677.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="ACG90W_002348"
     CDS             5384..6430
                     /locus_tag="ACG90W_002348"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0478678.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7685
                     /locus_tag="ACG90W_002349"
     CDS             6447..7685
                     /locus_tag="ACG90W_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0478679.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKRRKFQV"
     gene            complement(7858..8937)
                     /locus_tag="ACG90W_002350"
     CDS             complement(7858..8937)
                     /locus_tag="ACG90W_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0478680.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         2658 a         1759 c         1918 g         2687 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtagtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagacgt aaatttcaag
     7681 tttaagttag ccacccatgg tgacccggtt gctcgttacc accaaccgtc taatttttct
     7741 aatcgagtgg gcccttgaca atgagctgag gcatggtagt ctcccgactg gctggccccg
     7801 gccagctttc cagacacaag ccatgccgag ggaggctcgt tgtcaagggc ccacgtgtca
     7861 agccgtcaac cgttcctgcc taaatgcctg gacgaacgtg tcatgcggcg ttgcatagcc
     7921 caaaatcttt cgcgggtaat cgttcatcca acgctgaatc cgcaagattt ggttttcgct
     7981 cacgttgccg attgggctgc ccttggggat gaaacggcga atcagcttgt gttggttttc
     8041 gctcgtcccg cgctcccagg aagcataggg gtggctgaag tagatgtttg ccacatcctg
     8101 caaggcatcg tgcagtcccg cgaactccga gccgttgtcc gaggtaaccg tcttgaacag
     8161 ctggccgaag tacgcaccag cccgttctct cagctgtcgg accgcttggt tgaccgattc
     8221 ggcatccttc ccggccagct tcagaatgac ctcgaagcgt gtctgccgtt ccaccaacgt
     8281 cagcagcacg gcatccgact tggtcttgtt tcctacgacc gtgtcgactt cccaatggcc
     8341 gaaggttttc cgggtaccaa ccgcctccgg gcgctcttca atggatttcc ctagcacccg
     8401 tttattcggg cggttgccat gacgggacga tttcggtttg cgggacagtt tttctagcag
     8461 gtccatattc ttcgttttca tgatgccacg gtcgatccac tgatagaggg tggtggtccc
     8521 agggataatg gccgggtcga acaattcgtg ttttctggca aagcccacca ccacgtccgg
     8581 ggaccatttt tccaacagca tcatgtcgtc cgcccattca atgaaggcgt ccgtgtcggc
     8641 ccataagggc cgtctgccgc agttcaagcg gttcttctcg taggccgcct gcccggcatc
     8701 ggcgtcgtag acgtcatgat aatagtcgta gaccttcccg ttctgattct ggcggttaag
     8761 ctgagtaacg gtcccgcgtt tgatttcgtt gtttatggtc tgcggcacgc agcccaggac
     8821 atccgcaacc tgacggttgg aatagttttc ctttttcagg atggcgattt ggcaacgttc
     8881 tgagtaggtt aggtgttttc ccttgcgtga aactgtgtta tcatggtttt gcgtcatgtg
     8941 aattcatcct tgtctattga gagttgtggt aacttcaata taacatggaa ttcacgtggc
     9001 gtttttttat ttccaaatgg ct
//
LOCUS       DAZKOK010000114         8942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000114 DAZKOK010000000
VERSION     DAZKOK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.116"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG90W_002351"
     CDS             <1..260
                     /locus_tag="ACG90W_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478681.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACG90W_002352"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACG90W_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ACG90W_002353"
     CDS             complement(1379..1984)
                     /locus_tag="ACG90W_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0478682.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ACG90W_002354"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ACG90W_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2881
                     /locus_tag="ACG90W_002355"
                     /pseudo
     CDS             2666..2881
                     /locus_tag="ACG90W_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3019..3990
                     /locus_tag="ACG90W_002356"
     CDS             3019..3990
                     /locus_tag="ACG90W_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0478683.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ACG90W_002357"
     CDS             4130..5443
                     /locus_tag="ACG90W_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0478684.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ACG90W_002358"
     CDS             5486..6352
                     /locus_tag="ACG90W_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478685.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ACG90W_002359"
     CDS             6349..7197
                     /locus_tag="ACG90W_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478686.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ACG90W_002360"
     CDS             7211..8683
                     /locus_tag="ACG90W_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0478687.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="ACG90W_002361"
     CDS             8700..>8942
                     /locus_tag="ACG90W_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0478688.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAZKOK010000115         8890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000115 DAZKOK010000000
VERSION     DAZKOK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.118"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(494..718)
                     /locus_tag="ACG90W_002362"
     CDS             complement(494..718)
                     /locus_tag="ACG90W_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478689.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            909..3686
                     /locus_tag="ACG90W_002363"
     CDS             909..3686
                     /locus_tag="ACG90W_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0478690.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3767..4186
                     /locus_tag="ACG90W_002364"
     CDS             3767..4186
                     /locus_tag="ACG90W_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0478691.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4198..4668
                     /locus_tag="ACG90W_002365"
     CDS             4198..4668
                     /locus_tag="ACG90W_002365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0478692.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4685..5464
                     /locus_tag="ACG90W_002366"
     CDS             4685..5464
                     /locus_tag="ACG90W_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0478693.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5451..6272
                     /locus_tag="ACG90W_002367"
     CDS             5451..6272
                     /locus_tag="ACG90W_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0478694.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6289..7338
                     /locus_tag="ACG90W_002368"
     CDS             6289..7338
                     /locus_tag="ACG90W_002368"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0478695.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7351..8355
                     /locus_tag="ACG90W_002369"
     CDS             7351..8355
                     /locus_tag="ACG90W_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0478696.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2967 a         1470 c         1837 g         2616 t
ORIGIN      
        1 ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa aaaagtgggt
       61 tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg
      121 tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat ggtttggcgc
      181 agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca aaagctactt
      241 gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg tttctacctt
      301 gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa aaaggatggt
      361 gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc attatgccaa
      421 taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac aggaaagttg
      481 aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc aagataaaga
      541 tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa cgagtttgtt
      601 ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa gatccgatga
      661 tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc gatttcattg
      721 aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa actggaagga
      781 actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc tataataaga
      841 tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt ttctatgatg
      901 gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag aaccagacag
      961 cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta gctgatcatt
     1021 ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat gagcaactac
     1081 tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt gaaaaacaat
     1141 tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag gaacagcctt
     1201 ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc agtttgaaag
     1261 aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta ccacttttga
     1321 ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat cagtattgtc
     1381 tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt gcacaatatg
     1441 cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga gcgtacactg
     1501 gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc ttgtttttag
     1561 acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat cttgatcaag
     1621 gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt
     1681 ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga agaataccta
     1741 tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta gaactgattt
     1801 actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc agtggaccag
     1861 ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg aaaaatatcg
     1921 tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa atccatgtca
     1981 cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat acagcacagc
     2041 aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag caacagcccg
     2101 aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag aaaaataact
     2161 gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta tttgattttt
     2221 tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg acacaatcta
     2281 tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat ggaaacagtg
     2341 tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta ccggaagaac
     2401 atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct tatccatcta
     2461 gttatcacta tgtccaacga attctggaat taagtaagat caaattagat attgaagaca
     2521 caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa
     2581 aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc agtatggcaa
     2641 atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg ccactagatg
     2701 tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa
     2761 atggattgat catattagta gacatgggat ctctgaaaga aatccatcag ttttttaaaa
     2821 aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta gcaatcacag
     2881 taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag actgttcgac
     2941 agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa gcactcatta
     3001 ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta gaaaaaagct
     3061 tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta aaagataaga
     3121 aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc accacagatc
     3181 ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg gaaaagatgt
     3241 ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa atattcaatc
     3301 agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact attctagata
     3361 cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg cagttgaatc
     3421 gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac
     3481 gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg aagcagtgtc
     3541 aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag cattatagtg
     3601 tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa gatacagagt
     3661 ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa ttttggcatg
     3721 caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat
     3781 tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag agctgatcta
     3841 tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt ttgatttagc
     3901 ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg ttctgacgga
     3961 tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg atcatgtcta
     4021 tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt tggacgctga
     4081 agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt
     4141 ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg aagaaaaatg
     4201 attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt ttcatggaca
     4261 caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac gaatgagata
     4321 cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat caaatcaatt
     4381 gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt attcatcgta
     4441 gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat caaaaccatc
     4501 aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc ggtaaatatt
     4561 ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga agtagaaatc
     4621 cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga aaggaagaac
     4681 ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac aatcagaata
     4741 tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta caggaatcgt
     4801 gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag catttatcgg
     4861 atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga ttttagggac
     4921 ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag gtttgccgat
     4981 tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc caattatgaa
     5041 ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc atatgcactt
     5101 gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt cttatatggt
     5161 gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc aaaaggggtt
     5221 gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc gtttgctgat
     5281 caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt acttagagat
     5341 tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag tcaatattat
     5401 gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag atgaagaatt
     5461 ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt tggcgttctt
     5521 ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat gtttatgcaa
     5581 tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca gctttgaaac
     5641 ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta ggaatcacgg
     5701 ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg atcgatagta
     5761 tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt tggggaactt
     5821 tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat attttaggcc
     5881 ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat ctagcaacag
     5941 gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg
     6001 aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca atgactgcca
     6061 cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct gtgactgttc
     6121 aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt ggcatagtgt
     6181 tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta gctttctttg
     6241 gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat gtttacgatg
     6301 caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt
     6361 tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac gggttcatct
     6421 tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct tactgtcgat
     6481 attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat cgatactgtg
     6541 attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa aatgatacaa
     6601 aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact tgctttagaa
     6661 gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt tacaaaaggc
     6721 tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat aagtaaaaat
     6781 aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat aatcaaccat
     6841 ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag tttatttcgt
     6901 ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc taaagaagca
     6961 gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt agaagcttat
     7021 atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat tgaaggggaa
     7081 agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta cgttggagag
     7141 actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt agcaatagaa
     7201 tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt cgcttataaa
     7261 accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt tgatacagtc
     7321 ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc
     7381 aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat cgataaactt
     7441 tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta tgcttcctcg
     7501 atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt acagcatgct
     7561 gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt tattttgtcc
     7621 tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa gaaagtagga
     7681 gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct atgcgatttt
     7741 gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc agcagatcga
     7801 ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca gctcgaacgt
     7861 tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg cttatcattt
     7921 gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa tcaatgggga
     7981 gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc atcgaaatct
     8041 atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac aaccccagtc
     8101 acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc taaacttttg
     8161 ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt agaaggtatc
     8221 agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt gacacaacgc
     8281 atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg ctactatcgt
     8341 cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga gagcagcgat
     8401 aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa cacatttttt
     8461 gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc caaccatttt
     8521 ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt ccaagtacaa
     8581 cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa caaaagttcc
     8641 tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat ttagaaatgt
     8701 tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc ctaacctcct
     8761 aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca agcaaagcat
     8821 aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt tcaagtaaaa
     8881 acgataaaag
//
LOCUS       DAZKOK010000116         8832 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000116 DAZKOK010000000
VERSION     DAZKOK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.119"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG90W_002370"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG90W_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG90W_002371"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG90W_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG90W_002372"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG90W_002372"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0478697.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG90W_002373"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG90W_002373"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0478698.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG90W_002374"
     CDS             5704..6489
                     /locus_tag="ACG90W_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0478699.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG90W_002375"
     CDS             6502..7353
                     /locus_tag="ACG90W_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0478700.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG90W_002376"
     CDS             complement(7477..7929)
                     /locus_tag="ACG90W_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478701.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG90W_002377"
     CDS             complement(8394..8762)
                     /locus_tag="ACG90W_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0478702.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         2964 a         1486 c         1835 g         2547 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aa
//
LOCUS       DAZKOK010000117         8635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000117 DAZKOK010000000
VERSION     DAZKOK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.120"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..125
                     /locus_tag="ACG90W_002378"
     CDS             <1..125
                     /locus_tag="ACG90W_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0478703.1"
                     /translation="NQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(78..290)
                     /locus_tag="ACG90W_002379"
     CDS             complement(78..290)
                     /locus_tag="ACG90W_002379"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478704.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFLMILFVKLSETKLSIYVTQKNF"
     gene            complement(734..1855)
                     /locus_tag="ACG90W_002380"
     CDS             complement(734..1855)
                     /locus_tag="ACG90W_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0478705.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2183..3592
                     /gene="istA"
                     /locus_tag="ACG90W_002381"
     CDS             2183..3592
                     /gene="istA"
                     /locus_tag="ACG90W_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0478706.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3589..4317
                     /gene="istB"
                     /locus_tag="ACG90W_002382"
     CDS             3589..4317
                     /gene="istB"
                     /locus_tag="ACG90W_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0478707.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4445..5356)
                     /locus_tag="ACG90W_002383"
     CDS             complement(4445..5356)
                     /locus_tag="ACG90W_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0478708.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5373..6380)
                     /locus_tag="ACG90W_002384"
     CDS             complement(5373..6380)
                     /locus_tag="ACG90W_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0478709.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6377..8506)
                     /locus_tag="ACG90W_002385"
     CDS             complement(6377..8506)
                     /locus_tag="ACG90W_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0478710.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8511..8621)
                     /locus_tag="ACG90W_002386"
     CDS             complement(8511..8621)
                     /locus_tag="ACG90W_002386"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478711.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2651 a         1754 c         1503 g         2727 t
ORIGIN      
        1 tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct agaaaatcac
       61 taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag ttttgtctcg
      121 cttaatttga caaataatat cattaaaaag ttagtaaata attgccctct aagttccacg
      181 gtctgttcat taataacttt atatcctcgt ttttcaaaaa atggctttgc ggtaatggaa
      241 gcgtgggtag tgattttttg cataccagtt tctaaaacat ggttttccaa ttcatctgca
      301 attttagtcg caataccttt tccttgatgg ctatgttttt gtcacaactt cagtattcct
      361 gtaacaatac ggatttcagt atacaacaac agcttttgaa aagatacttg caaaaggatt
      421 aagatatcta tgacctgcta tgctcactga tgttgaaaag aatgttttat taggattgag
      481 gtaagcaaga tagtgaatat gtccggaaag cctgtaaaat aaggcttttc ggacatatgt
      541 gaagttataa aaagatagcg aaaaatctat ttagtatcac taatgttgcg aacatatcgt
      601 cagaatagtc tgacatttat ttcttgctaa aaatttcaaa aaagtcaagt gctgacgcag
      661 cggatgacta ttttccagaa aattttagta tgtattcaaa aatcaaacgt gacaacagct
      721 attaactaca tcctcatcca ggtttgtaac gtttgaacca ctcttctgcg ggctcaaaca
      781 ttaagtaatg gaaggtacgt ttcaattgaa acagcgtcgc tgtcgtattc aactcaattt
      841 ttatcacata agtgagaagc gttgcgatta aggtgaggat gacttgattt atcgcccctt
      901 gttcactatg tgaatatagt cgtttaatgg tcagattttg ttttaagtgt ttgaacaaca
      961 attcaatctg ccaacgggct tgatacatgt cggccacttc gtttggcgat acatcaaaga
     1021 gattggtcac cattcgcagg attttttgtc ccttggtaag aaccgttacc aacctaaaac
     1081 gctctgtcac atggttgaca ccgccgacaa gaatcacttg ttggtcactg attatcgtcc
     1141 catcacgggt tgttgtatct gctacttcaa tttgatccaa gatggtaatt tttgtattag
     1201 attttgtccg tgtaacgaag aaatagccgt cacgatttag tttgtctaac aacttgtaat
     1261 caaagtagcc gcgatccatc acatacgttg cttgcgtttt atcaacaaga acttcaaggt
     1321 gattacggtc atgctcactc gcatttgtca tcgtaaaact ttcaggatac tgattggttt
     1381 tatcaagata acagagattc aaatgtagct tgatgccaga ctttgttttc cgaaacttgg
     1441 cccatggaaa ccatgctttg ttcaacggaa tagtcgttga atcaatcaat tgtagactgg
     1501 ttgtttttga gcttggctgc tgagcagaaa ttctggcgac taattgcgta aaaatttcca
     1561 tcaatacttc ttgcttgatt tctggcgctc tacgagataa cgagctgtaa cacagggaat
     1621 caatgccaag ttccttgcag aggcgctgat ctaaaaaggc gcgatcaatc tcgcgatagc
     1681 ttggcaattc ttcataaacc gcatgaagta acactttaag cgtcgtttgg aagtctagtt
     1741 ttttactgta gaaatcaaag tcgctaatta agctttgaga gttttctgat aaaccagaaa
     1801 aatttattgc agaaaaccat ttttgaaatg aggtttttac gttatactta tccatgcgtg
     1861 agtccttttt attggtcttg gataagtcat ccgagaatag tataaaggac ttttttgcgt
     1921 ttggatagct ttgtattagg cttttgatgg gttaaaaagt ctttgcaaca ctagtaattt
     1981 agtatttatg tataaattga aagatatttt accaccactt agtcttctta aaagtgatcc
     2041 aacattttgt ttgtggcaga atttaactac acactaatgc agccgaaagt acacaactat
     2101 gcagcagttt gactgcgaac tggtttcctt ccatcataag tttgaatgac ttagtgatgg
     2161 aggtacagaa aggttgttaa ctttggatga tattaaaaca ataaaacatt tgagaaataa
     2221 tcgtgatcag tcaattgatc agataagaaa agcaatgaat atcaattggc gaacagcgaa
     2281 aaaatacgcc gatagtgatc aactattcat tacagatccc actcgaaaaa acaaatccat
     2341 gatggatgat ggttggggag aaatcatcgg tagttggcta gaagaagacc tacgtttacg
     2401 cagaaaactt cgtagaacgg ccaaagctat ccatgaacaa ttgaaacctt tgggtttccc
     2461 tggctcctat cgaacagttt gtgtatacat ttctcaatgg aaaaagcagc gttacgacga
     2521 aacgaattac caagctgtcg atcgtcttga tcatccgatc ggagaagcac aggtcgattt
     2581 tggaacaatg gaagttatgc atgaagatca catgaaagat atacaacttc ttatcatgtc
     2641 atttccctat agtaatgcga cgtccgcaag tgccatgcca gctcaaaacc aagagtgttt
     2701 cctggaagca ttaaaacaac tatttaatca atgcggcggc gttccaaaaa cgatcagaat
     2761 agataactta actccagcgg tgaaaaaaac aaaatcaaaa tttgaagaag cacaactaac
     2821 cgatgaattc ttacgtttca ccattcatta tggttgtgat gttcaagtct gtaacccacg
     2881 tagtgggcat gaaaaaggac atgtcgaaaa taaagtgggt tatgtccgat acaatttctt
     2941 ctcgacttcg ccaatcatgg actcttttga gtccctaaat aagcaacttg aagaacaatt
     3001 aaaagcagat cgaaaaagga cgcattattc aaaacatcaa ttaatcgaag atttgtggaa
     3061 gaaagaaaat aaagcgttac attctcttcc acaagaggat tacccaattt ttaaagaagt
     3121 ggaagctaaa ctaaatcgtt atcacgaaat gaaattggat caaacggtta ttcatattcc
     3181 taaagcatat ggctattctc aattgactgt tgttttgact tggaataaat tcaaagttct
     3241 tacgcctcat ggagaaatat taatgaacga atatcgcccc tatatgaaca aaaaaagatt
     3301 gatcccatgg agtgacatca tgaaacaatg gagtaataaa ttgaatgtcg ttagttattc
     3361 gaggtattgg aagtacttgc ctgcacgcct tcaaaattat ctagcgattg ataataatca
     3421 cctgaaatac agacggataa aacaattact aagtttatta gcgacctacg atatccaaca
     3481 aattaatgaa caattttacg aattagtcgt tcaagaagac gccccctctg actttgatgt
     3541 gaattggtca aactatgatg cattaacaaa aacaggaggt gtgcaaaaat gattggacag
     3601 acgttagaag attattgtaa aaagcttcga ttaagccata tcatcacgaa cataccaaga
     3661 gcttctttcg aagctccaga acaatttatt accgatttat tccgtttaga actggagacc
     3721 agacagcaga tcaaattaga acggatggaa aaattagcca aatttcctgt accaaaatcg
     3781 ctaaagaact atgaatggca cgaagatatt tttctaccat ctcatctaga taaggagacc
     3841 cttgtctctc ttgattttct aaaaaggcaa gaaaacattg tttgtgttgg ctctcctggt
     3901 acagggaaaa cacatctagc gatcgcatta gggagaaaag cctgtagtga aggtgtttca
     3961 accaaatttt tccgagttaa caatctcgta gagcaattag agaatgcatt aaaaaaacaa
     4021 cagctagcca gtttcaagaa gcgattcgaa acaacagaat taattattct tgatgaaatg
     4081 gggtacttac cattgacaaa agaaggagct gaacttctct tccaactgat ttcagattgg
     4141 tatgagcaaa agagcattat tattacctct aatttagaat tcagtcaatg gaatcgagtg
     4201 tttatggatt ctcgattaac agctgctctc gttgatcggc taatccatca cgctcacatt
     4261 atcagcttca aaggcgaaag tttcagattg aaaaattcat tatctaaatc atattgaagg
     4321 aaaaaactag ttgcagcaaa actctgcact ttcggctgca tttttttgta aaaagctatt
     4381 gcacaacaca aacatttgaa cgacgtaatt ccaaatgttg gattgctttt agataatctt
     4441 ttatttattc acaattgcta atagtccaat tttcattttt gtatagtttt aatctatact
     4501 gaaaaacact tgttgttttg gacaactcat caagatactc aacaccaaca atcacttgaa
     4561 tcgctttacc cttttgtttg taaataggat tcactaattc cacaaatttc aaattagtat
     4621 taattggttg catcgcgtct ttttcaacat aatgctctag ttctttttct gaggctgctg
     4681 gataaagcct gaaaaacgtc tctaaaaact cggtgatttc ttccgttgtc tttgaatcaa
     4741 tttttgaatc actttttact tgtcttactt tatgttttgc cttttctggc ttaccaatca
     4801 tagtgggtaa actcgttaca atatgattgc ctttttcatc ttcaaagatc atcagacgat
     4861 atgcagaaga aattgattgt gtctttttcc cttctgtaat cttttgatct accacaaatg
     4921 aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttactgat acaacctcgg
     4981 aactagtggg aatatccgct cgaaccatat cttggctcaa agcgagccct tcttctgtaa
     5041 ggtactgctc aagattagtc attctctttt caattacctc cttgttattc ttccatgaaa
     5101 aatattcttt cacaaaatct acagtaaaat tttctactcc agaggtattc actattttct
     5161 ctttcacgac cactttctca tgagttgtat gctgatcaat ggccgtgaag tttttgtaga
     5221 ttccaaaagc aaggctacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct
     5281 tgcccacgga tactactcgt tctttcttct tttttggaat cttttccttg ttacttcgct
     5341 caatttttat cttcattttc cattcctctt ttctattgtt tataccgacc agcaccaata
     5401 atgtgctgtt gccaatagct atcggtaaga ttggtccaac ccaccggatc tcctgcatga
     5461 aacattctgt tctctcctgc ataaatgccc acatgagtca cataagtacc ggcatcataa
     5521 gttgaatgaa agaaaatcaa atcgcctggt ttggcatctt ttaagtcaat atgagtcata
     5581 gcatcatatt gatcctgagc cacccgaggg agtttcaaac caactttggc ataacaccat
     5641 tgagttaaac cactacaatc aaacccagtt ccgggacttg acccaccaaa tacataagcc
     5701 gttccttcat acttgaacgc ctcgtcaaaa attccttgga ctgttttatc atcaaactgt
     5761 gttgtagtta ggtactgctt cactaataag caataaaaca tattgccata gttataccgc
     5821 cacccactat tttccttaat cgcgatggga ttgggataat cgactttttg accacccgat
     5881 ttttcttttg aaaaagattc ggctaaagca aaactatgct tctttccgtt tttcgcgaca
     5941 taatccaaat acccgtcacc atagttataa gcctgaatga tactatcttg atcgcatcct
     6001 agttctttgg aacacttaac caaagacgaa aaatagagac acacttgttt gatggatgcc
     6061 tcggtactta atgaattcgg tgataaacct gctgattcag aactttgcat gacatcttcc
     6121 attgtacctc cagattcgac ttgaataatc gccaaaagaa tcgaaacata ctctgttata
     6181 tctgattctt tggcatactt ttccaccatt gaacggtgtt tcaaaactac ttctgagaca
     6241 ctactacctc caggagaagt tccagaacta ccacttccct caccgtcctc accaaacaac
     6301 aaacccacac acatcaagat gcccacaaaa gagatggtca ggaaggttaa cgaaataata
     6361 ctcaattttt ttaagttcat cttttctcac ctggctttgg ttttgggcga gtgttctttt
     6421 ttctttgtac ttgttgattg gttcgctttg gaaaagtgaa tctctgtttc acttgttgaa
     6481 aggaattgtt cttgcttagt ggctgagtgc ttccgtttgg aatgacttct tgactagaaa
     6541 gtttccgttt gatttcagga ttcgaatgta cttgtaccct tggttttaaa gacctatttt
     6601 ctggtagttc atttttctgt ggttttatct ccttttttat cggaacattt tcccttcctt
     6661 tctcatgacg attccatgct tcgttcaata ctttgcgttt ttcatccatt tttgcgtctc
     6721 ttcgttgtcg ttgtttttct cgcaactccc ctcgatttgc tttttcttcg aaaggccctc
     6781 gcttaaagtc ctctggtact tttttcgtct tttccatgcc tttatgaagg ctgtatttca
     6841 agttagttgg tgcatcggtt acttgatttt ttgcttgttt cgctttgtcc ttcacgattt
     6901 cttttgtatc gagtactttt ccaacttttc taccaactaa atttattcgt gattctttct
     6961 tcgtgcccac tggttgttct tgttttttca gatcagaatt tagctcgtta atgcttctcg
     7021 cggaatggtt ctttctattg gacactctat tgatttttga actacctttc ccttttgtag
     7081 aactggtagc tagtttccca ccaactgctg caccgccacc aaaagcaaaa gttcgaccca
     7141 catttcgatt aagctgtcgc aattttcgat tcatcaacat ttgcggtttc cgcatgactc
     7201 gccttcccat tgactgacta tcattacttt gaaggttaaa catgctcata atgtcaccta
     7261 atttgaaata gataccggca aaggtcacaa tttgtagaaa agctaccata aagaagggat
     7321 aggtggccga aatattgaaa aacatcgtgg agatactgaa tgcagtcgta atgactaagg
     7381 taactcccgc tcgcatcata atcgtattga acaaacgaat aatcgccctt ttgcccaaac
     7441 tctcatacgt cggcaacatc gacaataaga aactaatggg cagaaacatc gcaaagataa
     7501 tgaaaaggat ttgtgagaac aacataatcc ctgtcagaag aaacacaaaa aaggaaatcc
     7561 caatattaaa caacactaaa aagaccacgg ttcctaaacg gttcatggtc ttggtaatac
     7621 tgagattcgc attgtcatta tcttcaatct ctgctttgac tgcttcttcc cgatccttac
     7681 ccttattttc atccggactc acggataaaa tcttattaac ccgaccctcg ccaatttctt
     7741 ctatattgga atcatcgaat tgtaatagta accagggttg ttgaacttga atcgaaaata
     7801 aactgtctcg aatcaaatcc acactatctt ttccctggct ttctgaatta ggtacaacaa
     7861 tcttggttcc aaggctaaga gccgcttcac taacgtctga gctaaagtca ttgatttttg
     7921 taatataggt gggcgcatac gcaatgaaag aacccgagag tagaaaaatc atcacaaaat
     7981 taatgacggt tcggactgct ttggtagttt ctcgttttaa taatccggta tacgccacat
     8041 aaactcctag agctaaaatc atcaataata ggaacccaaa gtagaatccc gaagtctgta
     8101 atccattttc agtgataccg gctaaagttt ggatattctt tccaatactt ttagccgtat
     8161 ccgaaatgaa atccaacttg taagcttctt gaaccacata gcccgtggcg tttgataaat
     8221 acagacttac tgtccaaata aaattggtaa tggcataaag accatacatc acgcttttcc
     8281 ctaagccatc cccccaattc caaggtaacc aatcccaaga actatccaca aagaaatcca
     8341 gttgataatt gttcagagaa tatttggaat aaaggtttcc ggcttcaact gtatcatcaa
     8401 ccagacctgt cgcctcagct accgtaccga ctaaactcaa tagaactgtc aataggacga
     8461 tgctaataaa aacgattccc acaatccgaa aaagtttctt tttcatcccc tcactccatt
     8521 ctctcttgaa ccggcggacg agtgtcaaaa gcatggaaga gttcttcaaa gactggatga
     8581 acttgaacca cacctactcg cccatacaag tcttgcatca agcattgacc gtttt
//
LOCUS       DAZKOK010000118         8561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000118 DAZKOK010000000
VERSION     DAZKOK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.121"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..2540)
                     /locus_tag="ACG90W_002387"
     CDS             complement(159..2540)
                     /locus_tag="ACG90W_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0478712.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2530..3177)
                     /gene="recU"
                     /locus_tag="ACG90W_002388"
     CDS             complement(2530..3177)
                     /gene="recU"
                     /locus_tag="ACG90W_002388"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0478713.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3241..3780
                     /locus_tag="ACG90W_002389"
     CDS             3241..3780
                     /locus_tag="ACG90W_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0478714.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3870..4289
                     /gene="gpsB"
                     /locus_tag="ACG90W_002390"
     CDS             3870..4289
                     /gene="gpsB"
                     /locus_tag="ACG90W_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0478715.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4311..4678
                     /gene="rnpB"
                     /locus_tag="ACG90W_002391"
     ncRNA           4311..4678
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG90W_002391"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4887..6053
                     /locus_tag="ACG90W_002392"
     CDS             4887..6053
                     /locus_tag="ACG90W_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0478716.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6099..7595
                     /locus_tag="ACG90W_002393"
     CDS             6099..7595
                     /locus_tag="ACG90W_002393"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0478717.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7676..8359
                     /locus_tag="ACG90W_002394"
     CDS             7676..8359
                     /locus_tag="ACG90W_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0478718.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8368..>8561
                     /locus_tag="ACG90W_002395"
     CDS             8368..>8561
                     /locus_tag="ACG90W_002395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478719.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2677 a         1565 c         1821 g         2498 t
ORIGIN      
        1 tttctaattt ttaagctaaa tgttagatca cacatgtata tgctggatag tcttataaaa
       61 aatcatgtaa tacagcttgg tacatgattt ttatttgtag ctgtttttta catagacaca
      121 aaaaagaaac cacgcttgta gacgtggttt cttttttact atctgtttcc agaagctgag
      181 gtgctgctgg atggtgtatg gtcatttgca gcatccgctc cgctagttgc tggctcactg
      241 gaagaagcag gaggagcaga tgaagacggt gtcgagcttt cttcggatga agaagaagga
      301 accgttgttg aagacgctgg ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca
      361 gcagttgatt cgctttgact agtagagcta gagctttgag tcgttgtctc tgttgttgag
      421 cttccaggag tctggacata gctggaagat ggaatagttg ttgatggtgt aattgcattc
      481 gatctagctg tatattgatc tttgtagtac agttcaccgc ccacacgaat taatccgctt
      541 ggcatctccc agtctgtatt tgtcacgtta gctgaaacat actgcataag ctcgcgataa
      601 acatcagagg ctacgtgtga cgattctgaa gtgacaggcg tcattttttt attgtaacct
      661 gtccacacgg aaatcgaata atttggcgta tagcccgcaa ataggatatc tggatacacc
      721 ccagaagaaa taccaagttt tgcatactcg tcatctgtgt aattggatgt tccggtctta
      781 ccggcttgat ataacccagc gatttgcgca ttcgtaccag ttccttcagt gatcgtatct
      841 tttaaaatgt ctgtgatcat ataagctgtt tctggagaca tggctgtttt tccatctggc
      901 tcataagttt cttctgttcc gtcttggaag acgattttat taacatattg aggtttgtaa
      961 taagtccctc cattggcgaa agccgcatag gctgctgcca tttttaagga agaagctccg
     1021 tattttgtcc catcttgttc ttccgtatta ctagagatcg cattcgattg atggatcgta
     1081 ctgtattcga tcccaagatt ttttaagaaa gaagccactt tatctgaacc gacttcattg
     1141 aataatttga ctgctggaac attacgggat aagtaaagtg cttggcgtaa agtaatggtg
     1201 cccatatatt gattatccca gtttcctaca ggagtcgaag tcccttcata gttatacggt
     1261 gcgtcagtaa tagtttttcc agtagaatat ttcaaatatt caaaagctgg tccgtaatct
     1321 gtgacaggtt tcatcgtaga accaaaatca cgggaagtat tcactgctaa gttgttgcct
     1381 aaagttacgt cttctgcgat gtgacgtccg ccaatctggg cttttacttt cccggtattg
     1441 gtgtcgataa gggtggaggc aacttgcatc tcgtcgtctg ggtaagagac atactgatcc
     1501 gtgttgacga tatcatatag tttcttttgg gcgtccagat ccaaattcgt ataaatctct
     1561 aaaccgtctg tgtagacatt cttatctgtt ttttcttgta cttcattgat gacttctttg
     1621 acataattat cgacgatctt agtattgtca tcggattgag ttagttcttg cagaccatcc
     1681 gttaccggta cattaacagc ttgatcatat tctgtttgag agattttttc gttttgaagc
     1741 atcgtataca aaacagtatc tcgtcttttc ttcgcctgat ctggataaac ataaggatca
     1801 taggcggaag gggcttgagg catcccagca agcaatgctg tttgcggtaa agaaagttct
     1861 gataatttct taccaaagta catctcagaa gctgtctcca tcccgtacaa gccgtttgac
     1921 atatacactt tatttacgta gtaggtcaag atctcttgtt tcgatttttt ttgttccaat
     1981 cttacagcca tccacgcttc ttgggccttg cgtttcagag tctgatcctc ggcactggtg
     2041 gagaagaagg aaagtttgat caattgctgt gtcaaagtac ttccgccttg cagtccgcca
     2101 gaggtaaagt tagagagggc agaaccgatg atccgaatag ggtcaacccc gatgtgttta
     2161 taaaaacggc gatcttctac cgacacgatc gcatcttcta atgttttcgg taattcattc
     2221 gcagaaattt tttcacgctt ttctgcgcct aaatcttcga atagttcgcc atcttgtgta
     2281 tatagtttgg aagatacggt agcgtctaat tttttatccg tcaattcggg cgcatctttt
     2341 gcgtaatacc agaaaagtcc tacacctgct aaaaagagga tacagaaaaa tgagagtatt
     2401 cctaaaagga tcttgataaa taatcctttt ttatgtgttc cagaggactt tccagaacct
     2461 ttgccagaat tcttcttcac actcttttta ggagtaggct gtttctgtcg tctagagctt
     2521 ctagtttgtt catttgccat tcttgctttc tccttcaata gataatgttg ataaatagaa
     2581 gtcaaccgcg tccaaataag gaatccgcgg agaaatacca gttttgatct ctattcctag
     2641 ttcttcgatc aaagataaag gtagtgattt gcgtcctttt tccttttggg cgtcccagta
     2701 agcaatcaaa tgaacactat tcaaataaaa acagcgcttc aaagacgaaa accataacaa
     2761 gacaaagcag accccatttt gttcgaggca tgctttcata tgttcgattt gatgggcatg
     2821 gaagttttta aagggaaaag atgttttgtt cttggtttct tttgcttcaa agtccaagta
     2881 aaagcctcga tagacgccat tatagtctgt agttgaggct tgggtaaagt aggcttcctt
     2941 gatcacagca gcacttctct taggatagtc tactttcacg atttggatag gggtaggctt
     3001 tttatggata accgccaacc cgcgggaaag ataatattta ttactttcat tgattgcttc
     3061 ttcaaagcgc atcccccgat tgccgaattc agtgatctga tttctttttt cttgaatttt
     3121 ttcgacggga gaggcattct tataataagg ttggccattt ggatagcgca ataccatctt
     3181 taatcacact cctaggccac attatagcaa aaaagcggat agaaagaaaa ggaatctgag
     3241 atggataagt caaaagtaat ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt
     3301 aaagaaaatg atcctaaaat ccaagttgtt aaaaatgttt taaaaaaaga actccatcag
     3361 ttagcagaag aagggctgga atgggtactt gtcagtggga atttgggcgt cgaactatgg
     3421 gctgtgcaaa tagtagcgga attgaaagaa gaatatccgg aactatctct tggtattttg
     3481 tatccttttt ctgattttgg taataactgg aatgaaggga accaagaaaa aaaacaatta
     3541 gcagaacaat tagcagatta tgtcgaagct gttagccatc aaccctacca atcacctagt
     3601 cagctaaaga atcacactcg ttttatactt gagcatacag tgggctgtct gctgatctat
     3661 gatgaagaat tccctggaaa aacgaagtat ttttttgaag atgcacagaa ttaccaagca
     3721 gatcatccct acgaaattcg gctgatcagt atggacgatc ttcaaaattt tagtgaataa
     3781 tcgatattgt gtttgattta tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta
     3841 ttacgtaaat gaaaactaga ggtgtaaata tggcgaattt ggtttatagt cctaaagaca
     3901 ttttacaaca agaattcaaa acaaaaatgc gcggctatga tcccgttgaa gttgatgaat
     3961 tcttagacaa tatcatcaaa gattatgaaa cttacagcaa agaactcctt gctttacaag
     4021 aagaaaacga tcgattgagt gcaaaagtag ctcaattatc taaaactcaa ggagcagctc
     4081 aaacacgcgt gcaacaaaca gaggcaccaa agagcgcagc agtgaccaat tttgatatct
     4141 taaaacgctt gtcaaatctt gaacgtgaag tatttggtaa aaagcttgat caacaagcaa
     4201 gtgcagtcaa accagcacaa cctaatccaa ataactacac aaatgcagac acaagcttag
     4261 atgataatga aaaaacacgt caattttaat ttttgattaa tgaatttgcg gttttcgggt
     4321 aatcgcggtc tgacttgatc aggctgagga aagtccatgc tcgcacaggc tgtgatgcct
     4381 gtagtgttcg tgcttagcga aaccataagc taaggtactc tttggagtaa cggcaggaaa
     4441 acaagctaag gcttcggcta tgcttaagta tccttgaaag tgccacagtg acgaagcggc
     4501 tggggaaacc catccggtgg aacgcggtaa acccctcgag cgagcaaccc aaacaatggt
     4561 aggggcgctt tttgtctgga agaagaacag gacaaaaagg cagtataact gcagatagat
     4621 ggttaccgct ttgttttttc tccctggaaa agcaaagtac agaacatggc ttatagaaaa
     4681 ccgcaaacat cagggaatta ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa
     4741 catttgaaag ctatttttca atacttcaga tgaatcgttc ttattacaaa acaaactttt
     4801 gtatcaccgg ttattcggaa tttgagtaaa agtgtggggt aggactgatg tcccacctcc
     4861 tttttttata aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa
     4921 ctgcggcaag cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat
     4981 gccaagttga gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta
     5041 atttatggtt acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata
     5101 cgtttgatga attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag
     5161 atgcagcttt tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt
     5221 gccaagcgat cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag
     5281 cttctgtacc actgacggaa actggcgcac attttcagtt ggaagtagct ttgctgaaag
     5341 accatgtgat ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct
     5401 tagaaaaagg tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa
     5461 attggcgaaa agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg
     5521 aagcagcctt aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat
     5581 cttgggattg gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag
     5641 cagattatga tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa
     5701 ttgccaaagc caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag
     5761 cagtcaaaga cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg
     5821 gagaacgtct aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat
     5881 tccgtccatt aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat
     5941 tttacggaca aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc
     6001 tattccagta ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact
     6061 aattgattca ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac
     6121 aagaagtaaa agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac
     6181 aaacaggtat gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca
     6241 gcatttattt ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg
     6301 aacatccgga agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt
     6361 atgatttgga acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt
     6421 cagcacacgg acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg
     6481 cattgaatca aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa
     6541 agacgaatta tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg
     6601 ataaggtatt tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa
     6661 aagggactga acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga
     6721 gatttgtagt gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg
     6781 acactgtcca cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat
     6841 ggaacgaaac agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta
     6901 tgtatgaaca agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa
     6961 tgggtatcca tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct
     7021 tctggaaaaa gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc
     7081 cttttgacaa attttatcgt tcattgaacc atacaaaatc aagtttggtt cgtatcgagg
     7141 cagacagttt gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt
     7201 tcaatgaagg ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt
     7261 atctgggaat acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag
     7321 gtgcaagctt tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac
     7381 ttcattcttt gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc
     7441 caatcaaaaa ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc
     7501 aattaatcat ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga
     7561 aagacctgta ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa
     7621 attcatctaa tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga
     7681 taaattacaa gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca
     7741 tgggagcgat cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc
     7801 gcaggcagac cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa
     7861 agtcgttaaa aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc
     7921 aatagtagag gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa
     7981 aaatctctct gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg
     8041 tatcgaagca ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg
     8101 gcacccgatt tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg
     8161 aaaaggcacg acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat
     8221 gaatactgat tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt
     8281 agaagaattt tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat
     8341 caaaaataac aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg
     8401 atcattttcc gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg
     8461 ttcagctccg agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt
     8521 gtaacttgtg attcggaatt ggcaggagaa caccgtttat t
//
LOCUS       DAZKOK010000119         8285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000119 DAZKOK010000000
VERSION     DAZKOK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.122"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            361..630
                     /gene="rpsN"
                     /locus_tag="ACG90W_002396"
     CDS             361..630
                     /gene="rpsN"
                     /locus_tag="ACG90W_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0478720.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            645..824
                     /gene="rpmF"
                     /locus_tag="ACG90W_002397"
     CDS             645..824
                     /gene="rpmF"
                     /locus_tag="ACG90W_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0478721.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            856..1233
                     /locus_tag="ACG90W_002398"
     CDS             856..1233
                     /locus_tag="ACG90W_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478722.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1254..1403
                     /gene="rpmG"
                     /locus_tag="ACG90W_002399"
     CDS             1254..1403
                     /gene="rpmG"
                     /locus_tag="ACG90W_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0478723.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1427..2410
                     /locus_tag="ACG90W_002400"
     CDS             1427..2410
                     /locus_tag="ACG90W_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0478724.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2440..2562
                     /locus_tag="ACG90W_002401"
     CDS             2440..2562
                     /locus_tag="ACG90W_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0478725.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2580..4103
                     /locus_tag="ACG90W_002402"
     CDS             2580..4103
                     /locus_tag="ACG90W_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0478726.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4127..4366
                     /locus_tag="ACG90W_002403"
     CDS             4127..4366
                     /locus_tag="ACG90W_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478727.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4559..5020)
                     /locus_tag="ACG90W_002404"
     CDS             complement(4559..5020)
                     /locus_tag="ACG90W_002404"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0478728.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5215..5433)
                     /locus_tag="ACG90W_002405"
     CDS             complement(5215..5433)
                     /locus_tag="ACG90W_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478729.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5551..7167
                     /locus_tag="ACG90W_002406"
     CDS             5551..7167
                     /locus_tag="ACG90W_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0478730.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7408..>8285)
                     /locus_tag="ACG90W_002407"
     CDS             complement(7408..>8285)
                     /locus_tag="ACG90W_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0478731.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT         2839 a         1276 c         1656 g         2514 t
ORIGIN      
        1 aaagttttaa aaaattggcg ataaaccagt taaacataaa agattgaata aaaagttata
       61 gtttctttca tattgccgtt ttaagtagag gaaaagctac ttaaaacggt atttgtgttt
      121 gattggacta ataaaagtga attcttattt ttcagagcct aaactgcata ttcttttaaa
      181 tgatttgatt atctctatgt accgataaat tcgttttcaa atagttatct attcatatga
      241 gttttatttc atgttattta ttgttgacaa gagcaactct tgttggtaaa gtatcagtgt
      301 caaaacgtaa cgattacgat ttgaggtgtc taaatcaaaa taaaaggagt ataaaggtat
      361 atggcaaaaa agtcgaaaat tgctaaagca aagaaacaaa tggcgatgat tgaaaagtat
      421 gcagataaac gtcaggaatt aaaagccgca ggagatcgca ccgctctcgc taaattgcca
      481 agagattcaa atccgaatcg tctgaggctt cgtgatcaga cggatggtcg tccaagaggg
      541 tatatgcgaa agttcggtat gtcccgtatt aagtttagag aacttgccca tcaagggttg
      601 attcctggag taaaaaaagc aagttggtaa aaggaggaaa tatcatggca gtaccagcaa
      661 gaaaaacatc taaggcgaag aaacggttaa gaagaactca tcaaactgta gtaaaaccag
      721 aaatttcatt tgacgaagcg aatggtgatt atagacgtag ccatcatgta tctttaaaag
      781 ggtactataa tggaaagaaa gtaattaaag gagcttcaaa atagtttaat atataacttt
      841 taggagggaa tcaaaatgga aattatttac ccacctttag ttgaagaagg attaaagtat
      901 tatcttgaaa cgacccagca atcgctagat aaatgcactt tttatcgttc gatggtagag
      961 agaggaatta ttactgaaac gggtttaccg acccaacagg caattgaaaa tggattagtc
     1021 aaagattact atgaggacca aggcttatca tttgatgatt ttttaagaat ctatccaatt
     1081 tttgaagagt atgacgagga gttatttcaa tgtatcgatg gttattggga gatacctgtt
     1141 gatatgaaag aaaatttagt ttcacaattg gaatctggcg agttaacttt tgaagatgca
     1201 caacaaattc aagcatatct tgaagatcga taagtattga aggaggaaga tttatgcgtc
     1261 aaaatattat tttggaatgt gtcgaaactg gagaacgtct gtatctcacc agcaaaaata
     1321 aacggaataa ccctgaacgg ttagaattaa aaaagtattc gccaaaattg cgcagacggg
     1381 caatttttaa agaagtgaaa tagctaagtg gtaaaaaggt gacaaaatgg gcataccagt
     1441 tacaatcgtt tctgggtttt taggggctgg aaaaacaacg ctaatcaatc aagccttgca
     1501 agtctcaccc taccccaggg aagaaattat tataattgaa aatgagtttg gtgaagttgg
     1561 gattgatcac aaattattag ttcatagcga ggaacgggtg ttacaactaa ataatggatg
     1621 tatgtgttgc agtcttcgca gtgatttatt agcaatattg atttctttac tggaaacaat
     1681 agaagaacat caacaaccaa tttcacaaat catcattgaa actacaggta tagcagatcc
     1741 ccaaccaatc attcaaacac ttttaacagc gcctcaacta agaggaagag tatatatcga
     1801 tagtttattg actgttattg atagtgacag ttttgaactt attgaaagag aaccacaagc
     1861 ccttaagcaa cttgtcatgg cagatcgcat ttttgtgtct agaaaacaaa acttgaattt
     1921 atctgcacta aaaaaattac agagagaaat caaaagtatt aaccctttat ccgatattcg
     1981 aatgttcaca catagtgaaa cgattcaaaa aaaggatttt tataatttga aaaaatttaa
     2041 tcatccctta gtttttgaaa gtgattcatc agaaaatcac tataatggca aatactatca
     2101 tcaacaccat caagtacatg agtttaaatc aattctctta acttcagatc aagacataaa
     2161 aaaagatttt ttactccaat ggattgactg gctgatttta accaaccaag ggagcctgta
     2221 tcgttttaaa ggattgattg ctttacaaga gcaggatttt atagttgcca tgcaagaggt
     2281 aaacgaacag gttaattttc aatacaccac tcgacaaaaa gaccaaacca ctattattct
     2341 aatcggaaaa atgttggatg aagaaaggat caaacagagt tttgataaat tactggcgga
     2401 aagcaaataa tgataagtag tgattggggg taatttctaa tggagaaaac ggatatatct
     2461 agtgcatatc gacgacttaa aagcccgaat attaaaacta gaaaaagggc attaaaaatt
     2521 atcaaagaac ataaacgaaa taaaatgaag aaattagcat aattaaggaa ggaaaaagaa
     2581 tgaagaaaat cagtgtagga cttattggga tagcagctct aggattattg ggagcttgct
     2641 ccagcactaa tgatgcaaaa gtatcgaatg ataaagatgg aaaattagaa attgtaacaa
     2701 cattctatcc aatgtatgat ttcaccaaaa acatcgtcgg cgatgaagcg aacgtggatt
     2761 taatggttcc agcgggatca gaacctcatg actatgagcc ctcagctaag gatatggcaa
     2821 aagctcatga tgctgatgtt tttgtatatc ataacgaaaa catggaatca tgggtaccaa
     2881 aagctaaaga aagttggaag aaagctgggc ctaatgtagt agaaggaaca aaagacatga
     2941 ttttattgcc aggtagtgaa gaagaagatc atgatcacgg agaagaagat caccatcatg
     3001 aactagatcc tcatacgtgg gtttcaccca aaatggcaat taaggaagtt tcaaatatca
     3061 aggatcagtt agttaaattg tatccgaaaa aagcaaaagt atttgagaca aatgctgaaa
     3121 agtatttgac taaactgaaa cggttggatg ctgactatac cactagtctt aaagaagcga
     3181 aacaaaaaag ttttgtgacg caacacgcag cctttggata tttagctttg gattatggat
     3241 tgaaacaagt tcctattgct ggactaagtc ctgaagaaga gccatcttct ggtcgattag
     3301 ctgaattaaa agagtatgtt aaaaaaaata agatcaatta tatttacttt gaaaagaatg
     3361 ctaatgataa aattgcaaaa actttggcca acgaagctgg gataaaattg gaagtactga
     3421 atcctcttga aagtcttaca aaagaacaaa tggataatgg tgaagattat gtttcagtaa
     3481 tggaagacaa tctaaaagcg ttagaaaaaa caactatggt tgctggtgaa gaggtagttc
     3541 cagaaaaaga agcgaaggat gaaaagacag ttgctaatgg gtactttaaa gatgccgatg
     3601 tgaaggatcg cgaattgagt gattatacag gcgaatggca atctgtttat ccgttgttaa
     3661 aagatggaat tttggatgaa gttttcgatt acaaagcaaa aatcaataaa gacatgacag
     3721 ctgccgagta caaagattat tatactactg gctataagac agatattgat actatcaaca
     3781 ttaaagacaa caccattgat tttgtagtca acggtgaaca tcatcaatac acatacaaat
     3841 atgttggata caaaattttg aattatgaaa aaggaaaccg tggtgttcgt tttaattttg
     3901 aaactgatga tgcaggtgct ggtcgtttta aatatattca atttagtgat catggaattg
     3961 caccaagtaa ggcagagcat ttccatattt tctttggtgg agaaagtcaa gaaaaactct
     4021 ataatgaatt gcataattgg cctactttct atccagcttc attgtctaaa catgaaattg
     4081 ctcaagaaat gatggctcat taaaattcaa tttttggagg aaatacatgg taagaaaagt
     4141 attattgtct atggattcca agaatattga tgcttatcgg cgttatcgat ttgcaaagca
     4201 aatgagatta aatgggagta cattagctga tattgctgca actttacatg ttagtattga
     4261 cacagttcat agagtattaa aacgggatcc tgattgttgt tttatcaagg tcaatactgg
     4321 aagaagtaga cgtttagcaa aacggcataa tagcttggcc aattaattga taataccaga
     4381 gtagatcatt tttcccttat ttggatttga tctgcttttt attatgtctt gaattgtcaa
     4441 atcaagcgca acactttatc aaagaagact tcataaggtg ttctccaatc taaacacttt
     4501 ctaggtctgt tattcagttt ttcaatcatc tgatttatcg ttgagttatc taccgtagct
     4561 atctctttcg attttggcaa gtattctcga atgagtctat tcgtatttta atttgttcat
     4621 tgttgccaag ggcatgagga tctggaaaat aaaagggcat attattcaat gcttctgtaa
     4681 ctgcgaaatg attggagaat tcttttcctc tatctggagt gatcgttttg accgcctcat
     4741 ttggaatact gcttaaaagt tcaatcattt tctcgctcac ataagcggct ttctttctgg
     4801 ggaccttctt tcctgctaaa aggaagcatg attttctgtc tgtcaaagtc actaaacaag
     4861 taccaccagt tttgccagct acaatatcgg ctttccaatg accaaaatca cttctatcat
     4921 ctgcggattt tggacggcca tggatcgaat gagtaaggca aatctttcca cgagactcga
     4981 catgattttt tgtatgtctt gtttttcctc gatggtgcaa tgaacggatc aggcctttat
     5041 tgccgtgtga gaggggttct gtatcgaaaa gatcattgca aattgcacgt tagatcgtga
     5101 taaaactaat tgaaaagtcc atatgttcat gcttcaaacg attcgagatt tcctctggcg
     5161 accactgaaa atcgacaaat aaatgacgaa caattgacca aactcacgga tcatttagaa
     5221 tgtgataacg tccgcatttt tttcttcgat aagcataatt tttttccgct ttggaaggtg
     5281 aatagttttt ttcttgtttc ttctcagttc atgtaaaata gtaaacgggc tatgctgaat
     5341 gtcgttgcca attatactaa tagaaaatcc ttcttcgtga agcagaaaga ttctttctcg
     5401 ttcgagtata gtaagatgac gatactggct cataattatt ttccttctag gagtgttctc
     5461 gacaaactta tactagttgg atatcgatta tgactcattt ttatgtgttg cacttaaagt
     5521 gtaaattcaa ggtatagaaa gaggttccct atgaaaaaaa tacctattac aatcttaact
     5581 ggctatcttg gttcaggaaa aacgatactt ctaaatcata ttctgtacgg tgatcacgga
     5641 aaggaaattg ctgtcattgt aaatgacata ggggaagtaa atgttgatgg ggtattagtc
     5701 gacaagaaag gttttaaaag aactggagaa aaacttgttt cgatgccgaa tggatgcatt
     5761 tgttgtacat tacgcgaaga tttgattgtt gaactagatc aattagtgca attttctgac
     5821 ttgaatcaaa ttgttattga agcttcagga atcagcaaac caattccaat tattcaagca
     5881 attatattag ctaaaaaccc tttagacatt gatttgaccc aacgattaga gattggtgcc
     5941 attattatag tagtggatgt aaaacgtttt tgggataatt tcgaaacggg tgaaaagatt
     6001 caggaaagaa aagctgaagg taatgtagtt gaagaagaac aggatattcg tggcttgtta
     6061 attgatcaat tcgagtgttg taacatatta cttttaaata atagtgactt agtgtcaacc
     6121 aagacgattt acaaaattaa cggtctaatt gctaagttac agtccaatgc caaaataatt
     6181 aggacaatta aaggtgaagt agacttatcg aaaattctaa atacaaatct ttttaatata
     6241 gatgagactt cctattcagt gggctggttc aaagagttag agcttggcta tcaaaatcat
     6301 attcctgaaa cagaagaata tggtatctca agttttatct atcgaaatcg gttactcctt
     6361 gacgcacaaa aatttgccca tcttacggat aaaaattttc ctggcaatat tacaagagca
     6421 aaagggtttc tttggtttgc ccaacatagt gatttttcga cgatctttga aacatcgggt
     6481 agctataaaa gtattgaatc attaaattat tgggtagcta gtttacctaa aattgagtgt
     6541 caaatgatta tacaaaaaaa tcccgaattg aaggaagcct ggcatccgtt gtttggtgaa
     6601 cgcattaata aactagtttt tattggtatg aagatggatc aacaatgtat taaggaacaa
     6661 cttgagggtt gtttaactga ttctcttaat gcagataatt tccgaaaaat ttcaccattt
     6721 ccatggaaaa agtctaatat gtttagccag atatcgttaa tgaaattttt aagaaaagct
     6781 ggtgtcaaga taggcgatgt cataattatt ggcattttga ttattacctc ttttattccc
     6841 ttatttgtat tctctataga aaaggagaat gaaacaactg aagatgctat caaatatgcc
     6901 atcgttcgaa ttgatggcaa ggaaatggat cgcttcgatt tggataagat caatcataaa
     6961 ctcgtgaatt atcatcctgc gaagggacaa tataatattg ttgagattaa agatggaaga
     7021 attcgagtta aagaagataa tagtcctgat caaatcgcgg ttagaactgg atggatctca
     7081 aaacccggtc aaacgagtat ttgtttaccc cataagttag ttattagtat tgagaaaaaa
     7141 gaaagcacag attactacat ttactaatct ggaaatagat gaggaggttt ttaataagaa
     7201 aaagatatta ttattaataa tagcaggggc tttattaagt gtgttaagcg cttgtggaca
     7261 agaaaaagaa gcggtgacaa caggcacaag tatctttaac gggacctgca atggtttagt
     7321 taacagtcgg ttagattact tattttggct cttcgtcaat gagtgttggt gaagaaattc
     7381 aatagaataa tcgagatact cacacggcta gacagctatc ttgtctagcc gtgttgttgt
     7441 tctggcttta gtcagttggg tagtattctt agttttctta aaatattttc ctcgatataa
     7501 aaagattcgt tctcggtagt tttgaaagtt tctatagcca taggcaattc gttttagtac
     7561 tttgatatgg ttattcaaac attctatagg gccgttggaa tagggaacgc gcaacgctcg
     7621 tttgatttct gtttggtatt tcttgaaggt cgtgaacact ggttggtagt gctctggcaa
     7681 ctccttgtag tcttctttta ggagcgcgac gaattcagga acatcttgtc ttctgataac
     7741 ggaaagaaaa tcctggtaga cttcgtagcc tcgctttaag gaatcatcat aagataatag
     7801 ccgatcgact atctctgctt ctgtcagatg atctcgaaat gacggacgcc agtagcgatg
     7861 ctgacctttc aattcccacg cattcttttg aagtagtttc cagtatttct ttaagtgctt
     7921 cgaccgacga tccccttttt taaaggtctt cattacttga attcggtggt tggtaaaagc
     7981 ccgaccaatg tgttggctaa tatgaaagcg atcggcgacg attcgggcat tcggaaaaca
     8041 ctctttcacc aatgtgcgat agggtgtata gaaatctgaa accactagac gcacttgttc
     8101 acggactttt atagggtagc gcaagaaata gtcgcgtaaa ggattgagtt gccgattttc
     8161 cacaatatca atcagttgat gggtcttgcc gtccatcatg acaaagctca tggctcccga
     8221 gacatttttc actgatttga attcatcgaa acacaccact tctggtaaca gctggctcaa
     8281 agaaa
//
LOCUS       DAZKOK010000120         8048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000120 DAZKOK010000000
VERSION     DAZKOK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.123"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACG90W_002408"
     CDS             complement(17..538)
                     /locus_tag="ACG90W_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0478732.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="ACG90W_002409"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="ACG90W_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0478733.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /gene="cdaA"
                     /locus_tag="ACG90W_002410"
     CDS             4618..5505
                     /gene="cdaA"
                     /locus_tag="ACG90W_002410"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0478734.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="ACG90W_002411"
     CDS             5502..6671
                     /locus_tag="ACG90W_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0478735.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..>8048
                     /gene="glmM"
                     /locus_tag="ACG90W_002412"
     CDS             6695..>8048
                     /gene="glmM"
                     /locus_tag="ACG90W_002412"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0478736.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         2580 a         1489 c         1827 g         2152 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gattaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggatt
//
LOCUS       DAZKOK010000121         7968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000121 DAZKOK010000000
VERSION     DAZKOK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.124"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..757
                     /locus_tag="ACG90W_002413"
     CDS             56..757
                     /locus_tag="ACG90W_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0478737.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1366..2382)
                     /gene="tsaD"
                     /locus_tag="ACG90W_002414"
     CDS             complement(1366..2382)
                     /gene="tsaD"
                     /locus_tag="ACG90W_002414"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0478738.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2379..2843)
                     /gene="rimI"
                     /locus_tag="ACG90W_002415"
     CDS             complement(2379..2843)
                     /gene="rimI"
                     /locus_tag="ACG90W_002415"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0478739.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2850..3392)
                     /gene="rimI"
                     /locus_tag="ACG90W_002416"
     CDS             complement(2850..3392)
                     /gene="rimI"
                     /locus_tag="ACG90W_002416"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0478740.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3376..4098)
                     /gene="tsaB"
                     /locus_tag="ACG90W_002417"
     CDS             complement(3376..4098)
                     /gene="tsaB"
                     /locus_tag="ACG90W_002417"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0478741.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4289..5269)
                     /locus_tag="ACG90W_002418"
     CDS             complement(4289..5269)
                     /locus_tag="ACG90W_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0478742.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5293..6777)
                     /gene="galT"
                     /locus_tag="ACG90W_002419"
     CDS             complement(5293..6777)
                     /gene="galT"
                     /locus_tag="ACG90W_002419"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0478743.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6789..7778)
                     /gene="galE"
                     /locus_tag="ACG90W_002420"
     CDS             complement(6789..7778)
                     /gene="galE"
                     /locus_tag="ACG90W_002420"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0478744.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2250 a         1707 c         1407 g         2604 t
ORIGIN      
        1 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
       61 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
      121 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
      181 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
      241 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
      301 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
      361 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
      421 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
      481 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
      541 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
      601 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
      661 agtaatcaaa caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt
      721 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
      781 cctgataaat atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
      841 gttttagatc actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
      901 cactgtttgc aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc
      961 tagattattc gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg
     1021 gataatgata aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa
     1081 aaaatagggc tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg
     1141 tcaactcttc ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg
     1201 ttccttgtga atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa
     1261 agcttgcata cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg
     1321 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac
     1381 caagcttgga tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg
     1441 tgctgctgct ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag
     1501 ttctacctca gggagtttag ttgccaaagc atcctgcaat ccttcacgca acccttgatt
     1561 ggccgctaca cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag
     1621 tgttttagta atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt
     1681 gtctagatct tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa
     1741 tccgctaaaa ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt
     1801 atcttttccc tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa
     1861 tactcttcca actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat
     1921 ttcatatgat ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag
     1981 tgccatcaat ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat
     2041 atggtttaca ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat
     2101 accaatcagc aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc
     2161 ttctgccgac acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac
     2221 gtgatggcgg ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg
     2281 agaagcaacg atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc
     2341 atcacaactt gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt
     2401 aactttctgc tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta
     2461 cctaatatac gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt
     2521 acttctaaaa aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa
     2581 tgccgtgcat gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact
     2641 tcatctatta ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc
     2701 agataatcag agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa
     2761 ccatgtggat aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca
     2821 aaatcttttt ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc
     2881 aacgcatctt cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc
     2941 aatttccgat aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc
     3001 tcacagcgat tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt
     3061 ttacgctgat atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct
     3121 cttgaaccga taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga
     3181 agcggtgact tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag
     3241 acttctcgtt cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg
     3301 taacgacgat cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag
     3361 cttctggcat acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc
     3421 caaccatttt tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc
     3481 tgcaactggt tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca
     3541 tgctggaact gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt
     3601 gtgacaatca ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa
     3661 atcagaagca gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg
     3721 gtatgcccca gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc
     3781 cacaccgatg caattagccg cgatggtttt cagactagat actcctacta gctctttatc
     3841 taatgtataa gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc
     3901 gggaccatca gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa
     3961 caattggtcg attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat
     4021 ttgtccaaga atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc
     4081 tattcctaat gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt
     4141 agcatacttt tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat
     4201 gattatctgc tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca
     4261 ctccattttc ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg
     4321 tttccacaat aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct
     4381 ccactgctag ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta
     4441 catatttcgc tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta
     4501 aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa
     4561 gcgcatcagg aacatgcgga ttcgttttta aatactgctt cactgcttga tatcctgact
     4621 cgacagaaaa atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt
     4681 gttgaaatgc cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg
     4741 ttccagcaag catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt
     4801 gaacacttga cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat
     4861 ctaccaaaag caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt
     4921 ttcctagtgc gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca
     4981 ataaagtaac gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat
     5041 aaagatcttc aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag
     5101 tatttgctgc atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt
     5161 tttgctttgt ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag
     5221 tagcaggaga aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct
     5281 ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc
     5341 tcgcttgaat actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg
     5401 tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc
     5461 ccacggacga tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc
     5521 tactagttct ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc
     5581 aatgttctcc tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg
     5641 ttcttctgaa acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct
     5701 tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga
     5761 tactgtttca tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag
     5821 caagtttttc tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc
     5881 gatgacttgc ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg
     5941 aaattcatga cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg
     6001 taagtcagca ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct
     6061 agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg
     6121 ttcattgtaa taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat
     6181 tcgaataatc cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt
     6241 ttccatgcaa agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat
     6301 ttgttttgga tctttttctg gcttagataa attgattgtg atttccagtt caccatactc
     6361 cgactctgcc ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt
     6421 cttacttaaa ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc
     6481 aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa
     6541 aatctcttgt tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag
     6601 ctgatccaat aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc
     6661 atcttctcct accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca
     6721 tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc
     6781 tcattcccct attcttcata accatttgga tggctgacat gccaatccca tgctgttttg
     6841 ataatcgctt tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca
     6901 cttgaagcga ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt
     6961 tcttttcctg tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg
     7021 ctgcctaaat tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata
     7081 tgtgcagcaa tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta
     7141 tcataatcgt ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg
     7201 attgggacaa gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct
     7261 ccggctacat tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac
     7321 ttcatcattt tttccatcat caatttactt tcaccatacg gatttttagg attagctggt
     7381 gtatcttctg tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat
     7441 acgattttat tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg
     7501 ttgttgttga aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca
     7561 aaatggatta ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc
     7621 cggatgtctc cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc
     7681 aaattatcta ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa
     7741 ccgatataac cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt
     7801 aaaattgtgt ttgttgattc tatataattt aaaaaacgaa gtttactttt atggtgaaaa
     7861 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
     7921 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagc
//
LOCUS       DAZKOK010000122         7804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000122 DAZKOK010000000
VERSION     DAZKOK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.125"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1416)
                     /locus_tag="ACG90W_002421"
     CDS             complement(52..1416)
                     /locus_tag="ACG90W_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0478745.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1413..2972)
                     /locus_tag="ACG90W_002422"
     CDS             complement(1413..2972)
                     /locus_tag="ACG90W_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478746.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3028..5508)
                     /locus_tag="ACG90W_002423"
     CDS             complement(3028..5508)
                     /locus_tag="ACG90W_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478747.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5492..5827)
                     /locus_tag="ACG90W_002424"
     CDS             complement(5492..5827)
                     /locus_tag="ACG90W_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478748.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5841..6875)
                     /locus_tag="ACG90W_002425"
     CDS             complement(5841..6875)
                     /locus_tag="ACG90W_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0478749.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6965..7645)
                     /locus_tag="ACG90W_002426"
     CDS             complement(6965..7645)
                     /locus_tag="ACG90W_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0478750.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2237 a         1560 c         1421 g         2586 t
ORIGIN      
        1 gattgattaa tatttattat atccccacac aataaaagac cgtgatattt ctcacagtct
       61 tttatctact ctattgctat ttgctctctt agctagtact ttaaaagcta actcattgag
      121 gtagtgaaaa cgagaggtgt attcagacag aaatccaaaa tcctcaactg ctagctgtgg
      181 aatcgtagtt ttgtgtttca cgatatccgc aagcaggttc aaatctgata ataagaattc
      241 ttggcttatc agctgtccgc caattatttc taatgtatcc ggatggtaga tcagctttgc
      301 atttactgtt ttgttttctt cgtcgtattg tggaaataga tcaaattctc ctgaacacga
      361 accggtattt tgttccaaga atggcgcttc atcttctgtc agccctacag ttcctaaaaa
      421 gtagtctata atgactgctg ctgttatctt ttgtctgagg tcgtaagcgg ttggctgacc
      481 actcaaagtt agcgctactg ctcttgccat atgtacagct cgagtaacta aaggtaaaaa
      541 gagatgccgt ttatttttgt caaaactgac tgggacgata tctccaatcg catagatgtc
      601 cggatcactt gtctgcagat attcattgac tatcacagtc tcatcctcat acaaagccac
      661 tttttctttt aacaatgtcg tattaggacg agtttgggca gaaaaaatga ccatatctgc
      721 ctttattgtt tgagacagaa gttgtacgga tactactttc tcttctgatt ttttccaatc
      781 gatcaagaat tcatttggat aaaaatttat ttgcttagtt tgcagctgat tttgaaaact
      841 gttgatcata tccttatcga aatatcttga taaaacattc ggcatataat caactagatg
      901 aataatgtat cctttatttc tcaaagcatc tgctaattcc aatccaatgt aaccagcacc
      961 gaagatcact acttcttttg cttcttttaa agcggcaagt acttgcttag attgcactag
     1021 atttttatag gtgtagacat tttcagcttc aatttgttta tacaaggtga caggagagga
     1081 acctgtcgct acgattaatt tatcataggt tatggtctcg ttttttcctt ctttggatgc
     1141 gaggatgagc tgatgtttgt ctggatcaag ttcagttact ctccattcgg tgatcagttt
     1201 tgccccactt tcttgaagag tccgcatgtt atgggcaact tcagctaaac tacgtatctt
     1261 tccttttaaa taaaggttga ttccatttga aacatagcct agtccatctt tatgatcttc
     1321 atcgatcagt aaaacttctg ctttcggttg aagttttctt agatttaggg cagctgtgat
     1381 gccttcatgg gaagcaccaa tgatcacgat tttcatgttt tattcatcct taaattcaat
     1441 ttttctaaat aacattcttt taaaaattgg ttcaaatagg ggaaatcata attatattcg
     1501 ttgctagtga atacaaaaac tttagcggac ttctgtgacg ggtactcttt caattggcta
     1561 gtcaaaatga tatcatactg cttttctttc tcgtattcga ctaaatcata tttgatcgta
     1621 ttcagcaatt gttcctggat gatttctttc acgatatccg ccattagcct ttcataagga
     1681 agatcataag caatttgcag acgtaattct aactgcttga tcagcgtccg taaaatagat
     1741 gcatagcgag caaataattc aaagcgggca ttttcctcgt ctacttcagt aaaatcaaca
     1801 atagcaaatg tttgtctgac caatctttca cataaaacca ggaattgttt atcttctact
     1861 tcagttaacc gctgttttaa tccttcttgg ccaaaaatat tcatccatcc tctaaaatag
     1921 gcaaaatgag cgtgattttg catcactgtc aacgcaatct tttgcgaaat ctcttctgga
     1981 aaaacataaa aagagaaata ttcttctatc gattgtttaa acgttcgatt caattgagct
     2041 actacgtcgt ctttgtattt ttcgcttatc aaaaactcct ggacatgcga gttatgtgta
     2101 tctaatgtaa agttactgat catgaaaaga taaaaaacca acgtttcatt ttgattccaa
     2161 ggaaaggcat attgaaagaa gtaacgggtc aaaaatgtat ggaggtccaa taaaaattgt
     2221 gtcgtttgaa acaatccaac aggaagattg ctataatctg gtttggattt tttcgtacag
     2281 cgcattcgct tcttcataat ggaaagccat atttcaaatt tcaatcgtcc ttcttctgta
     2341 atagcaaaac taaactcttt ttgcagcaga tctaaaaaag tgtttcctga taaatcactg
     2401 tattcttttt tgttttcaag atagggacgt ccaaaccaaa ataattggaa ataaaaataa
     2461 ctaatttgac tttctggacc ggtcagccgc ccgcttttta ttttcagatg aaactcattt
     2521 aaagaatcat ttaattcctt cactctgcga gacaaagtag ccgaactgat attaagttcc
     2581 atgacaagtt tctgccgatc aaatgagcca ttttccaaca gataaagaag tatctgatat
     2641 tttgtagaat agcgcaagta acaatgataa taaacatcga ctgctgctat atttccatgt
     2701 ctgattaata aaatattccc ttcttggata acaatttcca attgtttgat attttccaag
     2761 ttagcttgta aataaaacag atcttctttc aaggtagccc gatcaactga taagcgggta
     2821 agtacgttat taacggaaac ctctcctttt tcctctaata aaagttggaa aagtttgatt
     2881 tgccgttgtt catttttttc aagcaaactg aaaagttcca agtccactcc ttcaagcatt
     2941 tctgatttta acagcagaag tttccttctc atccttgaaa aagaggatct gtgacagtta
     3001 tcacagatcc atacaaattc taatcactta gctggttggc ccagctgtca gttcccatgt
     3061 gaccatgcct tgatacgttg cgccaggttg agcattcgta ctgctattta cctctaagag
     3121 taaaccatcg gtatcgggaa agtcaacgag aacctctcct ttagctgttc catcactctc
     3181 ataaatcaac gtgttttgac cttgctgaat caattgcttt gatccagaat gattgaagta
     3241 taattttgtt ccgcttaatt cttgaccttg atcattctta aataaagaag tcaatgtacc
     3301 tgtcagttgc caaggagacg tgcttggaag ccgagtatcc cgcacatcaa aagaaaaatc
     3361 gccttttgat tcataataac ttgtcttcaa cggtttgggg agatcgttac caaatgtaaa
     3421 atcatctggt acattaacta atgttaattc ttgtttgggg acaagaattg ttttagcaga
     3481 tgtttggaca actgggttag gaagcttagc accatctgct aatgtaatat tgctggaaag
     3541 tacagctgcc tttgtcagta tgctgacgga ttgtaaagaa gtatctacag tatttaattg
     3601 atagttgtaa gtaagatgaa tggtatctcc tattttgctc aatagatctg ctgggacaga
     3661 aactccttgt tgaatgccgg tatctgcgat agttgtacca tttaatttta aggaaccatt
     3721 gatataagta gtattttcag gaagtgagat cgtgattttt ttgtcggtta tcgcatcccc
     3781 agattttgtt aacgtgaatg tcacattttc ttgaatcgta tctcctgata gagcagacag
     3841 gggatctcct gtctcaggtt gtctgctaag gttttttagc tgactatctg cactcacaga
     3901 tggaaaatta gccactgtta tttgaggaag agtggtgatt ccagcttgtt cagactgcca
     3961 tttgtacgca aaagacggat acatgttagt aagtacatcg tccaccaagc gataatcaaa
     4021 ataatattgc cctgaagcca atgcaggtag tgtagaagta gctgtattta cacccccgtt
     4081 atttgattgg atcgtttgga aaagttgatc tgtccatcct gtcgctaatt tttgggttcc
     4141 tttcgaataa agcctgtaat ggatcgaacc cttttttagt tcagttgctg accaattggc
     4201 cgtgacttgg tacgatgcag aagaaaggag ctttccccac tgactatcgg ctgttaagtt
     4261 tgttggtaaa accgttccgt ctagcttgta ctgctccaat acctttaaag caacaggaga
     4321 aagcaatccg ccttgcgcat ctttcccact gtacacgata ttggctgtct gattcaatcc
     4381 acttgtgctt gctggagtat gagtcagtat tgctccttgt gtaacgtttt ttcctgtaaa
     4441 atagttgact acgcgatcaa agcgactaaa tggcaactga tcaaaacgtc cttcagtcag
     4501 ccccatcgtc aatgtccgac tattggtaga gcctggaagg ttctggttat ttccatccac
     4561 gtattctgtg gtatcagcaa atgtattata ttttcctgtt gaatcacttg gtgagatgta
     4621 actattccac gtatttatct ctgcacttgc agaaaatgca tatgctcctg gattcaagtt
     4681 tccgccagtt aatttaggat aaaatctacc tgcagctgga gctaaaatcg aattgaacat
     4741 acgatcttgg ataatattat tcgaatcata aaaatcaaca gaaaaaacgg cctttttaga
     4801 catagaaggt tgtaatttac ccttggtcaa aattttgttt ggactcgtat catatttatc
     4861 tccgttttct gtcatcttcg cgacattagc tgtaacatca aattgaagcg ggatatcgtc
     4921 cacgttgaat tcgcctcgcg taataatagc aataatatct gtcaatagag tatttgtatc
     4981 ccacgacccc atcccaaaac gcaaggtatg gcttcctgct gataataaac ttccgctagt
     5041 aaccttgatg ggggcaccgg caatatacaa gtaggcgttg ttccagtcaa ttgcttttaa
     5101 tacatcactg cttgatacat gttccggcaa attgatgatc gccacaaatg caaatccttg
     5161 tgcagtgata ttcaccaaat caagtacatt tgttagtttc acatggcttt cccaatgaaa
     5221 acggaccgtg ccacctttcc aaggatcatt tgaaccatcg ttcaaataag tagcagtcaa
     5281 tttaggcaat tggttagccc cagttagttg gacaagcttc ccgccattga ctgcactacg
     5341 tccaccattt ggaggtacga tattttcata cgtgtatgga tcataagaaa tggcaggagg
     5401 cgttgctcca acagcttctg ccgaaggtga aaagataaat atacttaaaa caaaaagcga
     5461 agtaaaaaag aaggagcgaa aaaatgaaaa tttattttgt agcaacatgt tcatcctcct
     5521 tttcctttct tctccataac aacaaccacg caagaagcca taattctata ccaatgatca
     5581 aataaagaaa acggatcttt tgttcattcg tctccgggaa tacgccatgt tgattagatt
     5641 ctttataaat cggtagctgt gtctgctgtt ttgatgcgcc attgactgaa ccacttggat
     5701 gcggaattgg acttagatca taaaatgtaa tcgtacctga acttttggta tctgcttgga
     5761 ctggctcttg ccagctgaag aacagcaaac ttgcaataat ccctcccaat aagatcgatg
     5821 ttttcaacga aatcacctca tcatgttgtt tctccttttt tctttttctt gtggacccac
     5881 aaaaacagca aaatcaacca taacactaga actgcagcaa ccaatcctgc ataaagccat
     5941 gggaaagatt gttcttgtac tgcaaaaacg gaattttcat ttactttttg tgctttttct
     6001 tttttgatcg taaagttttg tgtaaatcgc cattcattac tgtcgcctga ttttgctatg
     6061 atttctgcgc ggtaatcgcc ggcttttgct ttatcaccag ctaaatcaag attcaagtga
     6121 aaattcgaat ttggtgcaac taaaaatgca ttagacgtat tttcaatata aagctgattt
     6181 tcataaaaga tcttgctcgt aaatatcaaa ttgctgataa tcctaggctg aggattttgg
     6241 aaacgaagct gaacagttgg ctttgcgttg acctcgtcca aatctcctga taaaagtttc
     6301 agatccggtt ctatcgtctc ttgactttct tgtaaaacga ctgcgagatt ataagcaata
     6361 cgatttttga tttgtgctcc ttctgtagca ttttctgttt cagcttcttc tttaggtgaa
     6421 acatgaatac ccgctaatac acgtccctta aagctttttg ctgggatttt cacttctata
     6481 ggtacagttg tttctgcatg tgctgataca ttgatttcct ttttcgcaat gagcaaaacg
     6541 tcacggacat caaacggcaa tgtctcatct aacgttgggt ttttttgact ataatcgatc
     6601 gttccaccat cactcgttac cgccggattg acagagacct caaatgttcg ttcttcatct
     6661 gaagaattgc tgataattac ttctagtgtc tgattttgtt ccggtgccac taataaatca
     6721 aaataaccga ttgctttatc tatttggttc tctggaaata ccggtttgat tccaaagtct
     6781 cctgctgaat cctttgcttg gctgattgac ggaaaaatga aaaatagact taaaatagaa
     6841 aaaaacgaaa caaacagtcg tttactttga ttcattgtcc accacatcct ttcaacaaca
     6901 actgcggtaa aaaatccttc acctatagta ttttcaccgc agtttgttga atttatttca
     6961 tactctatgc tactgggcca gccacgaggt tccaagtcaa atcagccgtg aatgttccag
     7021 ctgctgcttt cggcactttc agtgtgatat ttgctggatc ataaacagaa agccatgttc
     7081 ccagaccttg atcttttgct gctgtaaata ttggtgtcgc tgcacttgct ttactcaacc
     7141 cagttacagt ttgactagtt ggtccagtgg attcgttatc tgacgtactt ttaacagtgg
     7201 atgttccaaa atctaattca gcgccttgca aaacttgtga accgtttaca aaatctgaaa
     7261 ccgaaactgt cacgttccac ccttgtgctt ggccatctgc acctacacct cgacgatctg
     7321 atacttggag ataaggtgta tcgttttttg aagcgtctac ttgatacgtt tttgttccgc
     7381 tttcaatctc gtgtgtacca aatggcaatt cgggaactac atccagtgtt aaaggacctg
     7441 tctggccagt tgctggattt tccggatctg ctggctctac tggttttgaa ggatcagtag
     7501 ggttaagagg ctccacggta tttgttggtt cagtaaaggt aatatctcct tttgaagtcg
     7561 cttgaccctc agcaaaagct ggtgttgcca agccgcctag aagaagtgtt gaaagtaata
     7621 atgtcccaat catgttcttt ttcataataa atcactcctt tttcttagtg acctaagtat
     7681 agtagagaca gtatgcaaaa aatcctcatg aattcatccg cttacttgaa attatgaccc
     7741 tttttgtata aaagagaaaa aaacaataaa atcgtttaca tgaatattct tcctttttta
     7801 ttaa
//
LOCUS       DAZKOK010000123         7599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000123 DAZKOK010000000
VERSION     DAZKOK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.126"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACG90W_002427"
     CDS             complement(136..426)
                     /locus_tag="ACG90W_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478751.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACG90W_002428"
     CDS             complement(544..804)
                     /locus_tag="ACG90W_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478752.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACG90W_002429"
     CDS             complement(941..1354)
                     /locus_tag="ACG90W_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0478753.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACG90W_002430"
     CDS             complement(1810..2295)
                     /locus_tag="ACG90W_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478754.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACG90W_002431"
     CDS             complement(2434..2649)
                     /locus_tag="ACG90W_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0478755.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACG90W_002432"
     CDS             2873..3673
                     /locus_tag="ACG90W_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0478756.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACG90W_002433"
     CDS             3692..5560
                     /locus_tag="ACG90W_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0478757.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACG90W_002434"
     CDS             5553..6044
                     /locus_tag="ACG90W_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0478758.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACG90W_002435"
     CDS             6032..6586
                     /locus_tag="ACG90W_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0478759.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="ACG90W_002436"
     CDS             6604..7599
                     /locus_tag="ACG90W_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0478760.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZKOK010000124         7585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000124 DAZKOK010000000
VERSION     DAZKOK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.127"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..529
                     /locus_tag="ACG90W_002437"
     CDS             56..529
                     /locus_tag="ACG90W_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0478761.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            526..2658
                     /gene="feoB"
                     /locus_tag="ACG90W_002438"
     CDS             526..2658
                     /gene="feoB"
                     /locus_tag="ACG90W_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0478762.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2660..2794
                     /locus_tag="ACG90W_002439"
     CDS             2660..2794
                     /locus_tag="ACG90W_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0478763.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2888..3532
                     /locus_tag="ACG90W_002440"
     CDS             2888..3532
                     /locus_tag="ACG90W_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478764.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3719..4912
                     /locus_tag="ACG90W_002441"
     CDS             3719..4912
                     /locus_tag="ACG90W_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0478765.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4905..6632
                     /locus_tag="ACG90W_002442"
     CDS             4905..6632
                     /locus_tag="ACG90W_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0478766.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7026..7223
                     /locus_tag="ACG90W_002443"
     CDS             7026..7223
                     /locus_tag="ACG90W_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0478767.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7225..7536
                     /locus_tag="ACG90W_002444"
     CDS             7225..7536
                     /locus_tag="ACG90W_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0478768.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
BASE COUNT         2492 a         1280 c         1667 g         2146 t
ORIGIN      
        1 gaacatataa tgaatgattt taatacatga tttatttcac aagagaggaa tcaatatgca
       61 gaccttagcg caggcaaagc agaatttcgt ttatcaagtc gaacgaatcg aaacagaaga
      121 aaaaacaaaa cgccatttgc aaaatatcgg gatcgttgcc ggaagtaaaa tcgtcgtcgt
      181 caatttatca ggcaacaatg ccattttact ggtaaaaagt catcgagtgg cagtaagtcg
      241 ggaaatacta ggtcaaatct ttgtcaataa aaagactgct tcctctgaaa gctggacttc
      301 tcttgatcaa ttaaaagcag gagagactgg gatcgtcgta gcaatccacg gacaaggcgc
      361 agtgaagcga cggttaatgg atatgggatt gacgcgaaat gtaaaaatag ttatccgaaa
      421 actagcccct ctgggagatc caatcgaact gacagtgcga gggtatgaac tgacgttacg
      481 aaagtcagag gccgaactaa tcttgataca aagggaggaa aaagaatgaa tatcgcgtta
      541 gtcggaaatc caaacagcgg gaaaactagc ttgttcaatc tcttgactgg ttcgaatcaa
      601 tatgtaggga attggccggg cgtaacagta gaaaaaaaag aaggatcgtt gaaaaaaaat
      661 ccgaacattc atattcagga cctacccggc atctattctt tgtctcctta tacaccagaa
      721 gaagtgattg caagagatta tttggttcag gaacagcctg aactgatcat caacattatt
      781 gatgctacca atttagaacg aaatctttat ttaactacgc agttattgga tttagacatt
      841 cctatgatca ttggtctgaa tatgatggat gtcatcaaaa aagaaggaaa aaaaatcaat
      901 ctagaaaagc tttcctatgg tcttggtgta ccagttttac caatcagcgt attgaaaaag
      961 caaggaatcg aaaaactaat cgaaaagatt caggagtctg caaaaaagcg gccttctatt
     1021 cctgttccct gtacatatga tccacgttta gaagcagctt tacatgaaat tgaagatgta
     1081 ctaggagaac aaagcggcaa gaaacgctgg tatgcaatga aatattttga acgagatcag
     1141 aaggtaagag aggattacga gatagcagtt tctcagcaaa aagagatcga gcaactgatc
     1201 cagttaacag agaaactatt ggacgacgat agtgagacga ttctggtcaa tgaacgttat
     1261 gaatttatta cccaactatg tacactctgt gttgtgtcaa atgattcttt ccaattatcg
     1321 atgagtgaca aaatcgatca gattgcaacg aatcgttggt tagccttgcc gatcttcgca
     1381 tttgtgatgt ggctgatcta ttatctggct attcaaacag taggaacaat ggggacagat
     1441 tggatcaatg acacattgtt tggtacatgg ttaccagaac atgttggcag attgttagct
     1501 gaatggcaag tagcgggatg gatgcaggag ctgatcttga acggcatcat cgcaggcgtg
     1561 ggagcagtgt taggatttct accgcaattg attatattgt ttttatgctt gagctttctg
     1621 gaagactgcg gatatatggc gaggatcgct tttgtcatgg atcgactttt caggaaattc
     1681 ggtctttcag gaaaatcctt cattcctatg ctgattgcta caggatgtgg agtgccaggc
     1741 gtcatggcca gtcgcacgat tgaaaacgag caagatcgtc gtttgacgat catggtcaca
     1801 accttcatgc cttgttctgc aaagttgccg attattgcct tagtagcagg ggcctttttt
     1861 cctcatcaat cttgggtagc tccttctgcc tattttcttg gtatgactgc aattattttc
     1921 tctggaatca gcttgaaaaa aacacgtttg tttgctggag atacagctcc ttttatcatg
     1981 gaattgcccg catatcattt tcctcaatgg tcagcggtcc taagacaaac ctatgaccgc
     2041 agcaagtctt ttgttaaaaa agcaggaacg attatctttg tttctagtat cgtgatctgg
     2101 ttcagctctt cttataattt ttatgctcaa cctgtttctg aagaccaaag tatcttagcc
     2161 tttttcgggc gaatcctggc agttgttttt cagccgcttg gttggggaaa ctggcaagga
     2221 acagtcgcag cgattacagg attggtcgct aaagaaaata ttattggcac atttggtatt
     2281 ttgtttggtc atgcggaagt atccgaaaat ggcagagaaa tctggcaagt gctacaacac
     2341 acatatactc ccgcagcagc ttattcattg ttagccttca atttactttg tgcgccttgt
     2401 tttgcggcaa ttggggctat ccatcgggaa atgaatgcca agaaatggac ttggatcgcc
     2461 ataggctatc aatgcggttt agcttattta gttagttttg tgatttatca gttgggacac
     2521 ttattggagg gtggacaggt agcgttaggg acgtatcttg ctattttact gatgatagga
     2581 ctcgtttatg ttttgttccg tcagccaaaa tccaagaacc agttttatac tatattgtca
     2641 ttagaaggag aggaatagga tggcgacatt cttattaagt gtgttaatct ttggaagtgc
     2701 aggcgcaatt gtctatactc gtttaaaatc cggtaagaat tgtgatgact gtcaaacagc
     2761 ttgcccagta aaaaaggaac agatcaagcg atgatcagtc gttgcaagag gctattctcc
     2821 ccttttttgc ttattttgct agagcatagt aagatgaggg aaagtaaaaa aagaaagagg
     2881 aaagaaaatg tctggaaaga aaaaaatcgt ccgcccgcgc taccagcaga ttgctgttga
     2941 tttagccgaa cgtatcgttg aaaaccgata taaagtgggt gagaagatcc atgcccgctc
     3001 aacacttgcc agtacgtaca atgcttctcc tgagactacc cgcaaggcta tcaatgtgct
     3061 ggtagatctt ggaatcatgg aagtacggca tggaagtggg gcatttgttg cttcgaagga
     3121 aaaagcaaaa gatttcctat tgcaatatca agatgtacaa tccatccaag atacaaaagc
     3181 agaaatcatg gcaagtgtgg agaagcaaca agatgagctg aatcattttt ctcaattatt
     3241 ggatcagctg gtcaatcaaa cgaagcgaat acataagatg aatcctttac ttccgtttga
     3301 attatcttta tcagaaaaag caatgcatct ggaccgttca attagcgagt tgaatatctg
     3361 gcaggcgact ggcgccacag ttatcgcaat ttctcataat gaagaactgc ttgtttcacc
     3421 tggcccctat gctaaattta cggcagggga catcgttcat tttattggaa atgaatatac
     3481 actacagcgt atgacaaact ttttttaccc ggagcatcag cttgcagaat gattgtgtga
     3541 aatattaaaa aaatattaac ttggctaaac cttttattta caaagattta gccaagtttt
     3601 tttcagtttg gacaagcaaa tttctttcat ttttgacaaa gtgactacat gatgttaaca
     3661 tagtgtggtc acttttttca gaaagaaaaa tgaatttaca taaaaaagga gaatttgttt
     3721 ggtaaaagta gaagtaaaac atttaacaaa gatatttggt aaaaagacac aagccgcttt
     3781 agatatgatg aatgatcacc agccgaaaac ggagatttta aagaaaaccg gtgcaacggt
     3841 tggtgtgtat gacgtgaatt ttgacgtaaa agaaggagaa atcttcgtga tcatggggct
     3901 ttccgggagt gggaaatcca cattgatccg tctattgaat cgtctgattg agccaacttc
     3961 gggcagtatc tatattgatg gagaagatgt atcgaagtta agcaaagaag aattgcgaga
     4021 agtccgtcgt cataaaatca atatggtttt ccaaaacttc ggtctgtttc cacatcgaac
     4081 gatcttggaa aatacagaat acgggttaga agtccgtggt gtacctaaag aagaacgaca
     4141 agaaaaagca gaaaaagcat tagaaaattc tagtttgctt tcatttaaag accaatatcc
     4201 tagtcagtta tctggtggga tgcaacaacg ggttggccta gccagagctt tagcaaatga
     4261 tcctgagatc ttgctgatgg acgaagcgtt ctcggctctt gatccgctga tccggcgaga
     4321 aatgcaagac gaattactgg acctgcaagc caatgttcaa aaaacgatta ttttcattac
     4381 ccatgatttg aatgaagctt tacggattgg ggacagaatc gccttgatga aagacggcga
     4441 aatcatgcag atcggaacag gtgaagaaat attgaccaat ccagcaaatg attatgtacg
     4501 tgaatttgtg gaagaagtcg atcgttccaa agtgcttacg gctcaaaaca tcatggtacc
     4561 tgcattgacg acgaatatcg aatctgatgg accaaacgtt gcattgacaa ggatgcgtaa
     4621 tgaagaagtc agcatgctga tggctgttga cagaaagaga cacctgaaag ggatcatcac
     4681 tgcagatcaa gcgttagaag ctaggaagca aaaacgtcca ttgatcgact tcctagatga
     4741 aaatgtgacg gtgattggga aagacatggt ggtaagtgat attttcaata ttatttatga
     4801 ctcaccgact ccacttgcag ttgtagagga tggaaaatta aaaggtgtcg tcatccgagg
     4861 tagtgtgatt gaagcattag cagaaacaag cgaggtgagt gaacatgaat aatctattga
     4921 actatcaatt accagtggca aactgggtag aaaatatcac agaatggttt acgacaactt
     4981 tttctggcct attttcattt ttacaaacga ttggacaggc agtgatgtcg gggattacaa
     5041 atttactgtt agtgattcca gctcctttgt ttatcttgtt attgacggta gcagctttct
     5101 ttatttctaa gaaacgtcca ggacttacgt tgttcacatt aatcggtcta tggtttattt
     5161 ataaccaagg tctatggaat gatttgatga atactgtcac acttgttctt ttatctagtg
     5221 tgatctcgat catcattggg gtaccattag ggatcttgat ggcaaaaagc agtaaagccc
     5281 aaagtattat caaaccgatc cttgacttta tgcagacaat gccaggattc gtttatttga
     5341 ttccagcagt tgcatttttc ggtatcggga tggttccagg ggtatttgcc tcagtcatct
     5401 ttgctctgcc accaaccgtt cgttttacga atttagggat tcgacaagtt cctaaagaat
     5461 tagtagaagc ttctgattca ttcgggagca caagtcgtca aaaactattc aaactagaat
     5521 tgcctttagc taaaagtacg atcatggcag ggatcaatca aacaacaatg ctgtcacttt
     5581 caatggtcgt tattgcctct atgatcggtg cacccggctt aggacgcggc gtattatcag
     5641 cattgcaaag agcacaagtc gggaatggat ttgtcaacgg ggttgcatta gttatcttag
     5701 caatcatcat tgaccgtttt acacagcatt tgaaccagcc aaacaataaa aaagctgcag
     5761 gagcaacaac acagaaaagt aaaaaacgtc aaggactgat cattggtgct gtagtagtag
     5821 tgatcttagg tgcgattggt atagggagtt tttcatctgc caaagaaaca aaacgtatca
     5881 acttatctta tgttgaatgg gatacagaag ttgcttcaac aaatgtagtc ggtgaagtat
     5941 tgaaacaaat gggctatgat gtaacgatga cgccattaga taattcaatc atgtggaaat
     6001 ctgtttcaaa tggcgagtca gatgccatgg tttcagcttg gctacctaaa actcatggtt
     6061 cacaatatgc acaatataaa gatcaagtcg aagacctagg tgccaatttg acaggagcaa
     6121 aagttggttt agcagtgcca gcctacatgg atgtaaattc gatcgatgag ttaaccgatc
     6181 aggcaggtaa gaaaatcata ggaatcgagc caggagctgg tgtagttacg gctgctgaaa
     6241 acacgatcca aaaatatgac aacttgaaag actggaaagt agagacatct tcttcaggag
     6301 caatgaccgt tgcactaggt caagcgatca aaaaacacga accaattgta gttacaggat
     6361 ggacacctca ttggatgttt gccaaatatg atctgaaata cttagaagat cctgaaaacg
     6421 gaatgggaag cgaagagcag atccatacga tggttcgaaa aggattgaaa gaagaccagc
     6481 cagaagctta caaagtatta gacaatttcc attggtcaga aaaagatatg gaaaaagtta
     6541 tgttggaaat caacaatgga aaagatccac agcaagcagc aaaagactgg atcaaagaga
     6601 accaagaatt agtcgaaagc tggaaaaaat aaaaagacag acacgaaata aatggagctt
     6661 ccaaaattgg atcaaatatc caaatttgga agctccatgt tcgtctctat cttttttgtc
     6721 tataaaaaaa gagaatgcgt agctcatctt cgaaacgtcc aaagacgccg acaaactatt
     6781 atgaatttat gggtgcgagt caaagagatc gatcgtaggt atgaaagact aatgtgatta
     6841 aagtttatta tgtacgaagt gcattctcaa aaaatattaa agaatataca tagtttatat
     6901 acttaaaagt gattaaattc ttattcagga attattttgc tacgtttcaa gaaggaattg
     6961 ttttttgaaa tttaatgaat gttagaataa tagtctaaaa acattaattt aggggtgatt
     7021 agattatgaa acatttaaaa attttgtcta ttaaagagac acaacttatc tatgggggta
     7081 ccactcatag tggaaaatat tatggaaatg gagtgtattg cactaaaaat aaatgtacgg
     7141 tcgattgggc caaggcaact acttgtattg caggaatgtc tataggtggt tttttaggtg
     7201 gagcaattcc agggaagtgc taaaatgaaa aaaaatgcta agcaaattgt tcatgaatta
     7261 tataatgata tatctataag taaagatcct aaatattctg atattcttga ggttttacaa
     7321 aaggtatatt taaaattaga aaaacaaaaa tatgaattag atcccggtcc tttaataaat
     7381 agattggtga attatctata ttttactgct tatactaata aaataagatt cactgaatat
     7441 caagaggaat taataagaaa tttgagtgaa attggaagaa ctgctggaat aaatggttta
     7501 tatcgagcag attatggaga taaatctcaa ttttaatgat acttataata ttaaatatgt
     7561 ctcaaaattt tttgattaga aaaat
//
LOCUS       DAZKOK010000125         7562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000125 DAZKOK010000000
VERSION     DAZKOK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.128"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..666)
                     /locus_tag="ACG90W_002445"
     CDS             complement(<1..666)
                     /locus_tag="ACG90W_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0478769.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQ"
     gene            complement(786..1433)
                     /locus_tag="ACG90W_002446"
     CDS             complement(786..1433)
                     /locus_tag="ACG90W_002446"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0478770.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1517..2944)
                     /gene="uxaC"
                     /locus_tag="ACG90W_002447"
     CDS             complement(1517..2944)
                     /gene="uxaC"
                     /locus_tag="ACG90W_002447"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0478771.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3018..4097)
                     /gene="uxuA"
                     /locus_tag="ACG90W_002448"
     CDS             complement(3018..4097)
                     /gene="uxuA"
                     /locus_tag="ACG90W_002448"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0478772.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4100..5731)
                     /locus_tag="ACG90W_002449"
     CDS             complement(4100..5731)
                     /locus_tag="ACG90W_002449"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0478773.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6035..6706
                     /locus_tag="ACG90W_002450"
     CDS             6035..6706
                     /locus_tag="ACG90W_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478774.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(6866..7057)
                     /locus_tag="ACG90W_002451"
                     /pseudo
     CDS             complement(6866..7057)
                     /locus_tag="ACG90W_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         2441 a         1483 c         1024 g         2614 t
ORIGIN      
        1 ttgagattgg tcaattctta ctcgttcagt agttagttta taacatatta aataacaaat
       61 aagtgcacat atcgaaaata ttcccgcaac aattgtaaaa gttgatccac cacgaactat
      121 ttgagcgcct gaatcatctc ttgtataaat aaataatggt gttaatacac caatgaccaa
      181 acttgctaaa gtagctccaa catttcggaa tgtcgatagc attgttcttt cttctgtaat
      241 agaagtaata gctgatgcca ttgatccata aggaatattt atagatgtgt aaaaaatact
      301 cccccataat atatacgtaa catacatgta tactacctta acgctcatag aagctgaaat
      361 catagaagtt tgatacatta aaaaacttgc taaagcgact ggaccactca ttcttaaaat
      421 ccacggttta aacttaccct gcttagttgc tttcgatcta tcaacaatac gccccatagt
      481 tacatcagta actgcatcga taaatcttgc tactaaaaat aatgttccaa cggcagctcc
      541 gcttatcccc aatacttttg tataaaagat cattaaaaaa gaactagcaa aaatgaaagt
      601 aaaatcgttt ccaaaatctc caaacatata tcctattttg tctcttatac caaattttct
      661 ttccatacaa ttctctccct tctgttgtta agcgttttca ttctgatgaa agtacgtact
      721 atactctgaa gcgtaaagag tcagtattta ttttttacat ttcagagtat tatttgtttt
      781 ttctcctaat aattctttat ctcataaaac ttatctatat atttactagc tactcgactt
      841 acttcttcaa aatcaccttt ttcaacagaa gccaataaat tcccacctac accaactgca
      901 acaactccta cttcaaacca atcttctaag ttctctatac ttactccgcc agtaggcatt
      961 atatttacgt gtggtaatgg agcttgatag gcttttacaa tggatggacc aaaagcagaa
     1021 cctgggaata gtttaatgat atctacgcca gcagtaagtg cctcctttat ctcagtaatt
     1081 gtcatacaac ctggtaaata aggaatttga tataaattac aaatctcagc tgtttgtcga
     1141 tcaaaagatg gactaacgat atattctgcc ccagccataa ttgcgatacg agcagtaaac
     1201 gcatctaaaa ccgtaccggc tccgattatt acatcttttt gatcagcata ttttttagca
     1261 atttctttga ttatttcttc agcattagga actgtaaaag ttagttcaat ccctttaatt
     1321 ccacccgcaa taattgaatt tattgcttta atggcctcat ccttattttt cccacgtaca
     1381 accgcaatga ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc
     1441 tccttccttt atggatacat actttgtcta gctgtactaa cttacaaaga ataaatttaa
     1501 gcttttattt caagtattat tttctacttc cgatttcaaa aaaattaaaa taattatacg
     1561 cattacgata acaaatattc tccactatct gactaagata catttcatca tctggaattc
     1621 ggcctgcttt aacccactcg cttagtaatt gacataatac tcgacgaaaa tactcatggc
     1681 gaggatagct tagaaaactt cgagaatcag tcaacatacc tgtaaaattc gacaacaaac
     1741 tttgttgtgc catcgtagtc aactgttgac gcattccttc agctgtatca ttgaaccacc
     1801 aagcacatcc caattggatt ttttgaatca tacccccttg gaagctttgc attgctgttg
     1861 ctagtggaag ccaatcagtt ggatttaatg aatataaaat tgtttttggt aagcacccac
     1921 tcttttcagc ggtatcgaac aatgctacaa cattttgtgt caaactagct tgatccccca
     1981 tcgaatcacc accgacatct actccaattt gttcctttaa tatacttgaa ttatttctta
     2041 aagcatttaa atgcatttgc atcgtccaac catatcttgt atactcagac atcaaatgta
     2101 attgaatacc tgtaacaaat tgtgcaattt ctgataaatg gaaatccgtt tttcctgcta
     2161 aagcttttat taacagattg tcgagtattt tttcatctgc tatttcataa aaataagtat
     2221 tcaaaccgtg atctgctaat cgacagccaa tttgatgaaa atgatccact cgttggctca
     2281 atgcttgctt caacccatta aagtctttga ttggtatagc cgctgtttga ctaagtttta
     2341 aaatatagtt cgaaaagcct gatgatttga tactccagac cttatctgga cggaaagtag
     2401 gtaatacctt gaaagaatta tcctgtttag aaagctcagt atgataccac aagtcatctg
     2461 taggatcatc cgttgtacag atggctttca catccatcat ctccaacaat tctttcgggc
     2521 gataggaatc cgtttgaatt ttttcgttag cacgtcgcca aatttctggt gcattcttct
     2581 cagaaattgt caaatcaata tcaaagtaac gttgtaattc caaatgagac cattcaaaaa
     2641 taggattacc tggcgccttc tccactgttt ttacaaaagc taaaaatttt tcataatcat
     2701 cacctggacc actgatcaac tcttcttcta taccattagc tcgcatcaat cgccatttat
     2761 aatgatctcc agctccttta tcatttaacc atacctgagt caaattcttg tattgcttat
     2821 tttctagcaa atatgctggg tctaaatgac aatgatagtc gataattggt tggtgttttg
     2881 caaatttatg aaaaagtctt ttagctattt cattctctaa taaaaagttc ttatctaaaa
     2941 acatatttaa acactccttt ccttccgtta gtttttagaa gagaaaattt ttcctctaaa
     3001 taaactccct tatataatta tttactcgcc cccttttcaa gcgcttccca aataccattt
     3061 aaataaactg cacctagagc tcggtcatat aatccataac caggtctacc ggtttctccc
     3121 caaatcatcc gaccatgatc tggacgaata tagccattaa atccattatc gtacattgtt
     3181 tttaatatct tatacatatc tagagaacca tattctgatt tatgcggcgc ttcgtaaaaa
     3241 tcgagtccat tgtcacttaa atatttgata ttacgaacat gtgcaaaagg aatccgatcc
     3301 cgttgacaaa attcagatag aatttcatag acatcattat ctggtcgttc tgcaatactt
     3361 ccagtacata atgtaatacc attagaagga gaatccacta cattacacaa ccaatctaaa
     3421 tcgtcacgat ttttaacaat acgaggtaat ccgaaaatgg aatatggtgg atcatctggg
     3481 tgaactgcca ttttaatccc aacttcttca catgtcggga taattgcttt gagaaaatat
     3541 gcaaagttct ctcgtaattt ctcttcatca acttctttat aggcttctaa taattgctta
     3601 atttgtgata accgatgagg ttcccaaccc ggcaattcaa aattattagt agaattttct
     3661 acttctctaa taatttcctc cggatcagta gggatatttt ttgaaatata ggctaacgta
     3721 gaagacccat catctaaacg ataatccaaa tccgatttta cccaatcaaa tactggcata
     3781 aaattataac agataacctc aatcccaaat tctgccaaat tgcgaatcgt ctgtttataa
     3841 ttctcaatgt attgatctcg tgttggtaaa ccaattttga tatcatcatg aacattaaca
     3901 ctctcaataa ctttcaaggt aagtccattg gcttcgactt catcttttaa agatttaatt
     3961 cgttctctcg gccaaacctc tccggctggt atatccatta atgttccaac aattcctttc
     4021 attgctggga tctgacgaat ttcttgtagt gagatttttt cttcatttgc cccataccat
     4081 ctaaaagtca tttccatagt tactttcctc caacttctgt tatatattta ttgattgttt
     4141 tacgaactgc ttgaggacct actagcattt cgcaaaagat tttttcagtt ttctttccta
     4201 ccccaacttg atataaatct aaaccaaaga tttctttatt ttctaaaatt tcctgtaccg
     4261 cacaatgaat ctttgaagga tcttgttcac ctaatttcaa tactgataat ttatcctgca
     4321 actctaccaa taacggatca ggacttggca caaaattttc cccttgatca tcgactgcca
     4381 gtaaataacg taaccaagca gcaatagcga taggaataaa tgttagattt tctgcatttc
     4441 cgtctttttc gacatacttt ttaattgttt caccatagcg aatcgctact ttttgagaag
     4501 tatctgttgc aattcgttgt ggtgtatctg gaatataggg attaggcaaa cgtttattaa
     4561 ttacttcatc aataaattgt tttggatcaa taatgcctgg attttcaact actggtaaac
     4621 cttctaaata acctattcct ttgattaaac caaccaaatc atcatcctgc atctctgctg
     4681 aaattgattg atatcctagc agacaacctg ttactgccaa agcagtatga agtggattca
     4741 agcttgtcgt aaccttcatg atgtctgctt tatcaaccgt ctctctatct gtcataatca
     4801 caccagcctt ttccaattta ggccggccat ttggaaaact atcttcaata actaaataat
     4861 gaactacctc agtattagtg aaagcagcaa tattggtatg tttttttgtg tgaatgatat
     4921 ctaaatcctc gagccccatc atcaatagat cttcctttac attctctgaa ggatttggcg
     4981 taattcgatc aatcatactc caaggaaaag tcactttttc tttatccttt aaataagcaa
     5041 taaatttttc agaaacatat ccttcttttt gccaagcttc tgcaatcgta aaaatactat
     5101 cttgaaaacg ttgaccattc tgtgagaaat tatcagtaga aaccatcgca atcggcaact
     5161 ctccagcatt aaatctgcta tataacaagg atgttacaat actcatcgta tgttgaggat
     5221 tgctaggtcc agatttgata tcagctttga ctaccgaaaa gaattgccct ttagaatctt
     5281 ttaaactgta acctttttca gtaatagtaa acgttgtaaa ctgtaattta ggatcttcaa
     5341 aatatttaac cactctttga tagtccgcta tattcttcgg attgcaatat aaactatcag
     5401 ccgttactgc caaaagtctt tttttgagtt caccatctcc actcataaca atttccaaaa
     5461 tgtcattgtt atagtcatga taagcttttt ccactacttc ttcatcaaat gtttcacaca
     5521 caactactcc tgtttctaat tcattttgat ctgctaagca ttgagcaatt tctgcatgaa
     5581 atcctcgata aagatttcct ccaccaaaat gaagccatac tggatgttgc tggccttctt
     5641 gcttaacttc tttgacatca aaacacggca gtagaatctg catttcattg aattcttcga
     5701 tagaattttg ccaattttta atcgaaacca taattaacct cctcaaactg atatgttagt
     5761 tttgatacat tttatatttt aaaacccatt gtaaccgttg tcaatataaa aagggaataa
     5821 acttgctttt ttctaccata tcagaatgat tgtatttttt attcagaaaa ataaaatact
     5881 ccttcttttt ggactgattt tatcgaaact tattttttct ttttttcatg tattctttta
     5941 tttttataac tagtgtatta cttcaaaatt tgatattata tggcatgaac agaattaaaa
     6001 tatcagttaa aataaaagtt aaggagcatt gtgtatgaca attaaaacta gtctccaaaa
     6061 tcaagcttac caatctattc gccgacaaat tatctatgct gacttagaac ctggaaaaaa
     6121 agtatcagaa aaaaaattag aagaaacact ttctattggt cgtacaccaa tacgtgaagc
     6181 actgattcaa ctccgacaac aaggtcttgt tgatacaatt cctcaaagcg gaaattatat
     6241 ctcattaatt gatttagatt cggccaaaaa tgctcgattt gtcagagaac atttggaacg
     6301 acaaattatg ttagagtgtt gtgccaaaat gaataaccaa aaaaaacgga ttttagaaac
     6361 tataatttca gaacaagaaa aagctgttag tcaaaaaaat gctcgtagtt tttttcataa
     6421 tgataattta ttccataaag cttgttttga aattgcagag cgagaagaag tatggcaatg
     6481 gttagaaaat aacaatacac acttagaacg atttcgatgg ctacgagtaa aaacggaagg
     6541 attaaagtgg gaaaaaatta tggaacaaca ttatcaacta tttgaggcat taacaaaaca
     6601 agatccagaa gaagctaact ttctaacatc gttgcatctt catttaatgc ttaatgagca
     6661 aaatattgtt ttagctactc atccaaaata tttcaaaaat atataacttt taactctttt
     6721 taaattaaat ggttgttctg atcagaaaaa tatcgcttgt tttcttatta tgtaatatat
     6781 ttcataaact attctgcata acaactattg tttgtacttt ttacattgga taactaatta
     6841 tgactgctga taacttttga tattaaacta gtatctccaa tacaaataaa aaatagatat
     6901 cttgttcatc aaaattagtt cgtggatact taccttcatt tacatcattc agcggtttcc
     6961 cattttcttt tattctatta tttcattttc taatagactt ggcttcattt cgcaatactt
     7021 tttttcaata ggcatcaaat tggtgttgtt tagatattta gttgtagttt tgtgacatga
     7081 taagtatgct cctttttctc ttttccacta atacaacgat caaagggtgc ttttttcaaa
     7141 aaactagaaa aatcaaaata agattcgtat tttgattcta tatatcctgt ttcaactaca
     7201 tcttatattt cacaatagaa taaatagcct tttgcatagc tgcttaactt ataagaaatt
     7261 gcaatttact tcttcaaagt gaaaacgaat atgagtaaaa taaaattatt cttcttacag
     7321 ataattaaaa atagcgcgta acaaaaatcg gcattcaatc acataattag gattgatgcc
     7381 gattacttat attctacgta tagttttagt attcttattt tttatcacca gtaaccaatt
     7441 gagaacagcc ctaaagcaga atagtctttt atctcaaaac gcttaatttc aggcttagga
     7501 atatgaataa tcttttgagt atattctttc tcttttaatc ttactgaaga aataccactg
     7561 cc
//
LOCUS       DAZKOK010000126         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000126 DAZKOK010000000
VERSION     DAZKOK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.129"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG90W_002452"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG90W_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0478775.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG90W_002453"
     CDS             1483..1935
                     /locus_tag="ACG90W_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478776.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG90W_002454"
     CDS             1949..2638
                     /locus_tag="ACG90W_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478777.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG90W_002455"
     CDS             2763..5114
                     /locus_tag="ACG90W_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0478778.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG90W_002456"
     CDS             5194..5589
                     /locus_tag="ACG90W_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478779.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG90W_002457"
     CDS             5914..6531
                     /locus_tag="ACG90W_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0478780.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG90W_002458"
     CDS             6572..6871
                     /locus_tag="ACG90W_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0478781.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG90W_002459"
     CDS             6905..7072
                     /locus_tag="ACG90W_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0478782.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG90W_002460"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG90W_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0478783.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKOK010000127         7489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000127 DAZKOK010000000
VERSION     DAZKOK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.130"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..403
                     /locus_tag="ACG90W_002461"
     CDS             86..403
                     /locus_tag="ACG90W_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478784.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            336..815
                     /locus_tag="ACG90W_002462"
     CDS             336..815
                     /locus_tag="ACG90W_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0478785.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            832..1008
                     /locus_tag="ACG90W_002463"
     CDS             832..1008
                     /locus_tag="ACG90W_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478786.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1265..1696
                     /locus_tag="ACG90W_002464"
     CDS             1265..1696
                     /locus_tag="ACG90W_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478787.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            1702..2019
                     /locus_tag="ACG90W_002465"
     CDS             1702..2019
                     /locus_tag="ACG90W_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0478788.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2138..2824
                     /locus_tag="ACG90W_002466"
     CDS             2138..2824
                     /locus_tag="ACG90W_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0478789.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            2799..4148
                     /locus_tag="ACG90W_002467"
     CDS             2799..4148
                     /locus_tag="ACG90W_002467"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0478790.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4151..4612
                     /locus_tag="ACG90W_002468"
     CDS             4151..4612
                     /locus_tag="ACG90W_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0478791.1"
                     /translation="MGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIARNKNN
                     EVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNWDKRR
                     NKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            4614..5114
                     /locus_tag="ACG90W_002469"
     CDS             4614..5114
                     /locus_tag="ACG90W_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0478792.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            5129..7441
                     /locus_tag="ACG90W_002470"
     CDS             5129..7441
                     /locus_tag="ACG90W_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0478793.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         2646 a         1286 c         1689 g         1868 t
ORIGIN      
        1 aaagacacga acgccggcaa gcatgatcgt gtcagggaaa taaactatct ttctatattt
       61 taccacagaa aggaatgaac cgtaaatgct aattgcaacg gatacactag accgcatctt
      121 tttaaaagac gaatacaaac tgcgcaatat agatgcgtca ggaattttag tatttgatct
      181 ttatgacaat ggaaaaattg gtatctatca agcaagtgat attgaagaaa caaaccttgc
      241 tttcgaacaa attgatgatt ctgtggaatt ggatttagat gaggcaatcc tagcttttga
      301 acaaattgca aaattattaa aggaggcaca aaagaatggc aactctttac caactcagcg
      361 agtcatatat caaagtcctg gaactggcag aacaattgga tgaagaaatt cttcgcgata
      421 ctctcgattc gattaatgaa gcgatcgagt ataaggcaga aaacttagca aaaatagtta
      481 aagaagtaga agggaaagct gagttaatcg atagtgaaat taaacgtctg caggaacgta
      541 agacatcgct tttgaacaat gctaagagta tcaagcacta tttacaagag gaaatggaaa
      601 agactggtaa aacgaagatt aaaggtgaat tattcaacat tggaattcaa aataatccag
      661 tatcggtcaa tgtaatcaat gagaacttaa ttccaaaagg attctttacc cctgtgcctc
      721 ccaaattgga taaaaagcaa ttgaaggagg aactgaagca cggagatatt cctggtgctg
      781 aactcgtcca aacaaaaggt ttgaggataa gatgatgaag gaggagaaat aatggaacac
      841 gaaaacgtaa aagacgcttt gaaagctgct atcgaaatcg cggaagctaa aggaataaag
      901 gttgacggca aaccagcaac tgtccatgac attcaaaatt tgacgaaaga acatctttat
      961 tttatcgccg acttgcttgg tttgtcggaa ttgtatttag acaaataacc ccacacggaa
     1021 ggagtggcta gggtggttgt attacaagta atagtctgcg taacattcat ttggtcgtgc
     1081 ataccggttt gtatatatat catgaagaag cttcgccctg agtcttacaa attatacgca
     1141 atttacagca taacagtagc tcttgcattt acattgtttg caatttctag gcttccgttt
     1201 taatcgtggg aatgaatttt taatgaacta aattatctat aaagcaaaaa aaggagaaac
     1261 aacattgaaa aacaaactag caaaaacaac agcaatcatc ggactagcac taggtagcgg
     1321 cgttatcggc tacgcagcaa gcaatgcgtt tcaagatttg gacacgatca aagcaaattt
     1381 caacacagtc ctacaatacg gtcaaacgaa atcgcaacgt gtgtcagaac ttgaatcaca
     1441 gctatccaac aacactcgca cacaggagca gctgaaagcc gaaattgagc aaatcaaatc
     1501 ggacaagcag aaggaaattg aagccaagca acgagaagtc gaacaaaagc aacaggagat
     1561 cgctacaaag caacaggaag ccgatagctt gcgccaacaa ctatccacag tgcaaaacga
     1621 caaggagcag ttagaacagc gcgtgagcga gttacggcag tatacggatc aaaaggtagg
     1681 ggagttgggg aaatgaaaga gttgactatt tatgacccaa atatttcatg ggctaaacac
     1741 acaattaaag tttcttttat gatttggggt tacaaaggat atgttactta caaagtgggc
     1801 ggtaacacta agggtttatc attaatagct attgattcag atgatctata tgatgctaat
     1861 ttcgaggata acccagttaa tttccgtgac cttgatgaag attggttctc tatggaactt
     1921 actaacgata aaggcgacag tacacttgta gaagatgaat tcgacagact aggtgattat
     1981 attgtcggtg ttgaaattat tgcgcacgaa ccagagtagg agagtgaata caaaatgatt
     2041 aaaagaatca ctatatcagt tgaagaagat agtggatcaa caatgcagca taccgacaat
     2101 cgaaaaactt ttttaaccaa tagaaaggag aattttgatg cagattaaga aggcgacgga
     2161 tatcgaacaa accaagggaa catatttgat ctacggtgct ccaggaaaag gtaaaacttc
     2221 aacggctaaa tattttccag ggaaaacact gatccttgat attgatcgga cttcaaaagt
     2281 ccttagaggt gagaaaaatg tcgatatcgt atatatcgac aacgaagata cctggaacga
     2341 ctggggcaac acgttggctg atttaactac caattatgta ggtgtttatg acaacgtggt
     2401 tgtcgataat gtcagcgaat tggaacggtg tattctgtcc agtttaggcg cagaaggaaa
     2461 aaacaacggc gttccttcgc aaggcgatta tcaatatatg caatttagaa tggtgaacag
     2521 cctacgctat atgaaaaatt tagacagcaa ccttatctgg accgcatggg aagaaatcga
     2581 tttgtggacg gattcggatg gatcttctta caacatcgct ttgccgcaga tcaatcgaaa
     2641 gattcgaaac aatattttgg gattatgtga cgttgttggt cgattgatgg taaaggaaga
     2701 cgatgaacgc ggatttattt tacaagccac agattcaacc tatgccaaaa atcaattgga
     2761 taatagatct ggctgtaagc aaagtgagct gattttagat ggaattgtac gactaccaga
     2821 atgatttagt cgatcgtgca agacaagcct atgttgatgg ttacaaggca ccatgtatcg
     2881 tatcaccgtg cggttctgga aaatcagtca tggttgcaga gatcgctaaa cgaaccacac
     2941 gaaaaggcaa cagagtgttg ttcttggtcc atcgtcgtga attgattgat cagatcaaag
     3001 atacgtttca aaagatgggc gtagataatc agcttgtaaa tttcggcatg gttcaaacag
     3061 tcgtacgaca tcttaaaacc atcaagaaac cacaactgat catcactgac gaaaaccatc
     3121 acgggttagc agcgtcctac agaaaaatct atgagtatta ttcggacgtt ccgcgattag
     3181 gttttaccgc gacacccatc cggttaaacg gtagtgggtt gggggatgtg aacgacttgc
     3241 taattgaagg agtatcggct aaatggttga ttgagaatca tagattagct ccttatgaat
     3301 attatgcacc taaattgatc gatacagccg aattaaagaa agcttcgact ggagatttta
     3361 caaagaaatc aatggataag gcagtgaaga atactattta tggcgacgtg ttgaaacatt
     3421 ataaaacgtt agcagaaggt gaacaggcaa tagcatactg tcacagtatc gatgcaagta
     3481 aacacactgc tgaaatattc aacgatgctg gttataaagc aacacatatc gatgcaaaaa
     3541 ctccaaaaga tgaacgtgca gatattatcg aagcgttcag aaatcatgaa attaaaatcc
     3601 tatgcaatgt tgatttgatc ggagaagggt ttgatgttcc tgattgttcg actgtaatta
     3661 tgttgagacc tactcaatcg ttatcattgt acattcagca atcgatgagg gggatgcgtt
     3721 accgaccaga taaaacgtca atcatcattg accatgtggg taatgtgaac cgttttggat
     3781 tgcctgatat ggatcgtgaa tggagtttag atacaaaaaa gaaaacaaaa actgatagtg
     3841 atttatctat cgttcaatgc gcgttttgtt ttggtgctta cgaacggcca aaaggcgata
     3901 atatttgtcc gtattgcgga gagttgcagc cgattgaaga acgaaagtca gagttagaaa
     3961 tcgatgaatc ggctgaatta atgaaagtcg gagaaacgaa aattactttg aattttgaga
     4021 acaataaata ttacaacatg acggaagatg aagcagaaag tatagaggac ttatatgcca
     4081 tagcaagagc taaaggattt aaacctggtt gggcttatat ggctgctaag aggaaggggt
     4141 ggttgtagga atgggcggta tttttggtaa aaaagcagaa gatgttcaag gttcaataat
     4201 aaatggcgta acaataatag gtgatactgg aaaaagagat agttcaggca atcaaattgt
     4261 aatcgctaga aataaaaata acgaagtttt tgaatcttct ctatctaata taaaaagagg
     4321 tgttgttaac gggacaggcg aagagagtgg ctacgttaat ttaaaaaaag ctaatgaaaa
     4381 ataccatgtt gaaggaactt acgttaaggc tttgtctgtc ggtggaacaa aaggcgttaa
     4441 ttgggataaa cgaagaaata aatggaaagc acgattatat ttcaaaggag aatatgtctt
     4501 acaaaaggat ttcaacacca aagaagaagc aatccaagca cgacttgcag cagaagaaaa
     4561 atatttcaaa cctatcttag aaaaatatga aaataaaaag gagaatgatt aacatgacag
     4621 gatttaactt agacttttca aaagcgcaac aagggaacga aatcaaagac ggtacgtacg
     4681 aagtggtcgt aaacaaagct gtagaaaatg caactaaatc aggcgcagaa tttatcgata
     4741 tcgatttgat cgttcgtaac gatgtggatc aaccgttcca aaataaacac attttcgcaa
     4801 aaatctggaa agccaaagct accggcaaat ataacgaagg tatgatcatg gcaattgcac
     4861 aagcgctaca gttggaagac ggaaaatcat ataacgggtt tgatgaattg ctggcggact
     4921 ttgttttgaa aaccgcctca gtacgcgtga aaactgaaga gtcaaatgga tataaaaacg
     4981 tcaatgtgaa gtcttgggat aaaacgaaca cacggggcgt aatgaatcac caattcaaaa
     5041 atggtgatga accatccttc ggaccggaac gttcaagagc gacaacggtt agaaatgaca
     5101 atcttccatt ttaattgagg tgtaatcaat gtatgaaaaa attccaaaag agcttaaaaa
     5161 tctcaaacaa tggtgtgttt ataagctagt ttgggatgaa aagcggcaga agcacacgaa
     5221 gatcccttat aacgccaaca acgggcataa agccaaatcc aatgacgaga gtacctggtc
     5281 tgattttcag accgctctcg ctgctattga tcactacgga atgagtggac tggggttctt
     5341 tttcaaacca ccttatttcg gcattgatat cgataatgct gaaggtgaag ttgagcgatt
     5401 taaatcaggt gatatagaag aaaatattat ctatgaattt atcgaaagca tgagatccta
     5461 tgctgagtat tctcaatcag gtactggcat ccacattatt gctcgcggag aattgcccgg
     5521 cggaagacgc aggaaaggtg acgtagaaat gtaccagaac ggccgattct tcgttatgac
     5581 tggaaatgcc gcatcaaaat atttagaaat aactgagccg aatccaaagg atatcaaacg
     5641 cctatatgat cgctacgttg gagatgaaaa aatcatccaa ttcaaagaag aaaatccgct
     5701 gatgaatacc gttgatttac cggttgaaga aattatcaaa cgtgctgaat cgtcttccca
     5761 aggggcaaga ttcaaagtct ttatgaacgg cggttgggaa gctttttatt cctcgcaatc
     5821 cgaagcggat atggcgttcg ccaacgactt ggctttttgg accggtagag actttgaaaa
     5881 aatggatgag attttcagag cgtctgcaat gatccgtagc aaatatgacc aaaaaagggg
     5941 atcggcaact tacggagaat ctctactaaa taaggcaatt gctgatacaa attccgtcta
     6001 caaccccaaa cggaaatctg attttaagat ttttatcaaa gaccaggaac agccaaaaga
     6061 ggaaaaatac tattcgtacg acgacaccgg caacgcggat cggtttactg atatctacgg
     6121 aacgttagtc aaatattcgt acattgataa atcttggtat tactacgatg ggaaagtttg
     6181 gctacaggac aataccggcg aagttcggaa aatgatcgat acgacagtag acatcatggg
     6241 aaaagaaccg ttaacgatcc ctgaaggcgc agacgatgaa accaaagaag ctttgatgaa
     6301 agcgaaagaa aaacacgtca agcgttctcg cagcaatgcc ggaaaaaacg ctatgatgga
     6361 cgaattgaaa catcgactat ccgtattgcc ggaagagttc gacaaagata aaacgctgtt
     6421 caacacacaa agcggctatc tgtctttgca tgacggtctt ctacacgagc atgagataga
     6481 caaaatgttt acacgtgttt caaatgtcga gtacacagaa tcggtcgatt gtcctatgtg
     6541 ggaagaattt attaatcaga tttttgacaa tgatcaagaa ttaatcagat atatccagaa
     6601 atgtgtcgga tattccctga ccggatctac cagagagcaa tgtatgttca tcctctacgg
     6661 tcatgggtca aatggaaagt cggtattttt ggaaattatt tctgaattga tgggaaacta
     6721 tgcgatgact atgcaggcac aaacgatcat ggtcaagcaa agccaatcat cagcaaacag
     6781 tgatattgca cgattgaaag gtgcgcggtt ggtcacttcg tcagaaccga atgaaggcgt
     6841 ccgactggac gaaggtctgg tcaaacaatt gactggtggt gataaagtca cggctcggca
     6901 cctttatgga aaagaattcg agttcgaacc tgaattcaag ttgtggctgg caacgaacca
     6961 caagcccatc atccgtggga ccgatgacgg catctggcga cggttaaatt taattccatt
     7021 cactgtccaa atcccggatc acaaaaagga taaaaatctg aaatttaaat tgcagacgga
     7081 gttgcaaggg atccttaaat gggcaatcga tggttgcctg ttgtggcaga gagagggctt
     7141 ggagaaacca aagtcggttg ttgcagcgag tcaggattat cgaaacgaaa tggatcaaat
     7201 aggtacattt atcgaaacgt gttgcgaaac aggtccagga ctaaaaatat caggcggaga
     7261 attatacaaa gtttatcgtg aatgggcatc agataatggc gagcatactt tcactaacac
     7321 gaagtttggc agagagattt caaagaaata tagcaaagaa aaagctggtg gatttatggt
     7381 ttataaagga atcacattaa aaccaagaaa atacgataac gttagagaac tttttaaata
     7441 aatctggagg gttctggagg gttgagaacg aaaccctcca acccttaga
//
LOCUS       DAZKOK010000128         7432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000128 DAZKOK010000000
VERSION     DAZKOK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.131"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG90W_002471"
     CDS             14..1141
                     /locus_tag="ACG90W_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0478794.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG90W_002472"
     CDS             complement(1359..2501)
                     /locus_tag="ACG90W_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0478795.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG90W_002473"
     CDS             complement(2506..2691)
                     /locus_tag="ACG90W_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0478796.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACG90W_002474"
     CDS             complement(3045..3290)
                     /locus_tag="ACG90W_002474"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478797.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="ACG90W_002475"
     CDS             complement(3284..3685)
                     /locus_tag="ACG90W_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0478798.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG90W_002476"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG90W_002476"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0478799.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..>7432)
                     /locus_tag="ACG90W_002477"
     CDS             complement(5703..>7432)
                     /locus_tag="ACG90W_002477"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFQ0478800.1"
                     /translation="LDEDAISWIEETIDSMTLEEKIGQLFVNMGSSRDKNYLSNMVNQ
                     YHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKIG
                     ATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQNPDKVLEL
                     SLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFGKVYSGLIEA
                     GLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASH
                     MLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIITDDRLLDALKR
                     ILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAITLVKDKQDIWPI
                     VPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKG
                     LSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWPASKGTPDIPFY
                     IHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGVSPIDA
                     YCGLKDTFI"
BASE COUNT         2388 a         1427 c         1032 g         2585 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa at
//
LOCUS       DAZKOK010000129         7248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000129 DAZKOK010000000
VERSION     DAZKOK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.132"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1463)
                     /locus_tag="ACG90W_002478"
                     /pseudo
     CDS             complement(37..1463)
                     /locus_tag="ACG90W_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1706..2161
                     /gene="tnpA"
                     /locus_tag="ACG90W_002479"
     CDS             1706..2161
                     /gene="tnpA"
                     /locus_tag="ACG90W_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0478801.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2426..2524
                     /locus_tag="ACG90W_002480"
                     /pseudo
     CDS             2426..2524
                     /locus_tag="ACG90W_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2747..2977)
                     /locus_tag="ACG90W_002481"
     CDS             complement(2747..2977)
                     /locus_tag="ACG90W_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0478802.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(3207..4025)
                     /locus_tag="ACG90W_002482"
     CDS             complement(3207..4025)
                     /locus_tag="ACG90W_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0478803.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            4186..4875
                     /locus_tag="ACG90W_002483"
     CDS             4186..4875
                     /locus_tag="ACG90W_002483"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0478804.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4889..6391
                     /locus_tag="ACG90W_002484"
     CDS             4889..6391
                     /locus_tag="ACG90W_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0478805.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6404..6880
                     /locus_tag="ACG90W_002485"
     CDS             6404..6880
                     /locus_tag="ACG90W_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0478806.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6977..7087)
                     /locus_tag="ACG90W_002486"
                     /pseudo
     CDS             complement(6977..7087)
                     /locus_tag="ACG90W_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2342 a         1149 c         1308 g         2449 t
ORIGIN      
        1 aatccactcc ttttttcatg ctctaaaatg atatttttac acaactgtta cggttgtttt
       61 acgaaattgg acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta
      121 atagatatag ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg
      181 aaaaatctct tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt
      241 ccctttccga gacatgcttt gaaagatttt gtactccttc aatttgtttc tataagcttt
      301 catttgatac gcccaacctt gatctgagtg aaacgtacga cgatagggac aatcgttagt
      361 ggtctggata gcttcctcca aaccttccat taccgctaat gcattgggct tttcagagat
      421 acgataggat aaaatctcac tattgaacat atccataaaa ggatctagat acaatttctt
      481 ttgacggata atgcctttgg catccacttc atggtattta aattcagttg tatccgtcgt
      541 gattttctga tggcagatac tggtgtagaa acgacgatgg atccgatttt tggctactgc
      601 tccgacttgt cctcgataag aattgtagcg ccgtgattta cgtgtgtatg tcgttacttt
      661 tattcctagc ttcttgatca ggcgctgaac tttcttttta ttgatagaga aaccgcggtt
      721 tcttagttca ttggtcatcc gtaaacagcc ataatccttg tgcttctccc gaactttaag
      781 catttccgct tcaatctctt gatctggatt tttacggtcc aatcgttttt gccagtacat
      841 aaagattgcc ttagggaact ttaaagtttc tagaagctca actaatttaa acggtcctcg
      901 gaggcttgcg atgattcgtg cgattgcttc attcgtcgtt gttgggcttc ctgttttcgc
      961 agcttcctca attcttttaa aaaggcattt tcaatttgaa gggaacggac tcgtttctcc
     1021 aactcctcga tgcgttcacg ttctttagac gttgctttag ttgtagattt tttcttattt
     1081 ttgggttcat ttttcttaga caagataggt ggacatcctt tcgtttttga gagtccatcg
     1141 atcccaccct cacggaattg acgcatccaa ctagcgatca aattagggtt gttcatgtta
     1201 agtgtgttgg caacttcacg ataggacagc tcacttgtta gatataactc tatcgcatcc
     1261 agcttaaatt gaacagaata ttttttcttt tgtcgctttc ttactagacc ctcttcacca
     1321 aattcttgat aggcattgat ccagcgcttc acttgtgatg gagccttaat ggaatatttc
     1381 tttgccagat aatcacttcc gcctttacca tccaagtaat catgaacgat ctttaattta
     1441 agttcaaaag tatacttagc cataaaaaat accgacctcc aaaagttaga ttttttggtc
     1501 taacttttgg gggtcggtac aaaacttaaa agttttagac ccttttattt tcttaatgac
     1561 ttcagtcagt tttgtgcgta aagcgtgcga aacaagaaaa ccgcgcgttt ctacgcgtgg
     1621 aagaagtatc tttagcgata aaaactcctt tcgatatact agtgagtggc taccaaccgc
     1681 tcatcagatc gaaaggagtt ttttcatgtc aaatgacgat aaaagtttag catatactcg
     1741 ttggaattgt aagtatcata ttatttttac accaaaatat caacgaaaag taatctatgg
     1801 gcaattacga aaagatcttg gaaaaatact taggaagttg tgcgaaatga aagatgtgga
     1861 aatcatagaa gcacatgcaa tgctagatca tattcacatg cttgttcgag taccaccaaa
     1921 aatatgtata tccagtttta tggggtatct gaaaggtcgt agtgcggtac taatccacga
     1981 gcagcacgca aatctcaaat ataaatacgg aaacaaatcc ttttggtcga agagatacta
     2041 tgtgagtaca gtcggattga atcaaaagac aaatgaaaaa tacatccgtg aacaagaagc
     2101 tgatgatcgc attcgatata ttataagtaa aagagagtac gtagaaccat ttaaaaaata
     2161 aagagaagta agtgagtgaa gatgagcagt tggcggtctt ttaaaaatcc ccttttaagg
     2221 ggcagcgagt gtcgacccct tctaggggga ttaaaaatac acccgtcctc acgggtgtat
     2281 gggttactga tagacttgat tttaactggt gtcagaaact tatgtccaga aagggtgatg
     2341 aaattttggc aatttacggc tattcaagaa ttagcacaag caaccaagat tacaaaacac
     2401 aaattcaaac attagaaaat gctggcgcag aaataatctt cctagaaaat cactggatta
     2461 tcgaacacct ttggaagtat ttttgagtta cgtaagtatt gatgatctgt ctaacttaat
     2521 ttgacaatta agagtatatt tacttcagat ggtaaggcaa caaaataaaa tacaataggc
     2581 agatgaacta ataaataaaa tctgtattaa atacagataa attaatataa aaatcaaaag
     2641 gatcataaac ttttaacaag tgatgaaatg ggtggaaatt gattcttata gacagtttgt
     2701 ctcataacat tgaagtattt tgcaatttta gcattgaaac acttttttaa tcttcaacgt
     2761 tgttcttata aacaagatga ttgggaatct tttgaattaa cactgtatag cttagaacac
     2821 cctttataga aaacattcgc ttagcaatct taataattcc ttgtgcccga tgtcgtttag
     2881 attcagtagg ttcctgctca tcgatcatgg aaaaaatttg aaggatcaga tccttaataa
     2941 atttatttaa gagaggatga tccattgctt catccatact cgataaacta gtgataatga
     3001 gctacacgtt tttctttcaa gttgatcaat agcttccacg ataaaaatat ctttagataa
     3061 aatcaagaac cgtaggaact acagggatat ttggtcaata agagccaccg ctattagtaa
     3121 aaaataagct atcaagtatg ctctcatccc aagaattgcc tacttcaatc gttaaaaacc
     3181 gttaggttta gctaagtggg ggtagttcac tcaatacttt tatcaataat agagcgcaaa
     3241 actttttctc ttaattcaac tgaatcaata ttattgtttt gttcataacc atgaactaat
     3301 agatcacaaa tataaagttg tgatattttt ccagctaaag atccaccatt aagaaattct
     3361 tcaatggctg tttgtaaaac taagtctgca gactttccta taggtgaatg aatatagtta
     3421 gttatagcaa taatttttgc gccatttttt ttagctattt tcaatgaatc ataagtatct
     3481 ttcgttttcc cagataaaga aaaaatgatt actaaatcat taactgttaa taaagaagcg
     3541 acttgggctt gaaaatgagg gtctagtaca gcttttgctt gaactcctac gcgaagaaac
     3601 atattttcta aatcaagact agtatttcca ctagatccca ctccaaatat atatatgttt
     3661 tttgattgag tgatcatttt aatggcttca ttcaattttt cttcgtctaa aagcctaata
     3721 gtggaataga gagcttctgt taaactatta gcaatttcat catagtactt tacactagtc
     3781 ggatgttctt ttttttgact aaagtcttcc tttgcaattt caatttttaa atcagaaaaa
     3841 ccagaaaaac caattttttg acaaaagcga ataatagtag catcgccaac ttttgtgttt
     3901 ttcttaatat cactcatcgt actataaata atagttttcc cagaatttaa aataaaatca
     3961 gcgacttttt tttcagactt cgttaaaaat ggataatttt ctttaattaa atagatataa
     4021 ctcatacaaa ctcctctaca atttcaactt ttctccatta tactccaaaa taataaaaaa
     4081 aactcctaaa tactccattt ttatctttac ttttatgaaa gcggatgcta tattgttttt
     4141 ggagttatta tccaaaaata ataaaaaatt ggagcgttta ggataatgga taaaaagagg
     4201 tttttagatc ttatttcaaa aggtttgatt gtttcatgtc aagcattacc tggtgaacca
     4261 ttttacaaag aggaagaaag tttaatgcct tattttgctt tggctgctca aagggctgga
     4321 gcagttggta tacgtgcaaa ttctgttcga gatattattg aaattagaaa gtgtgtatca
     4381 ttaccaatga ttggaattat taaaagagat tatccaccag aatctccatt cattactgcc
     4441 actatgagag aagtagatga attagtagaa accggtgttg aggtaatcgc tcttgattgc
     4501 actcttagaa atcgacatga tggactatct ataaattctt ttattactaa aataaaagaa
     4561 aaatatcctc atcagttatt tatggcagat atttctacat ttgaagaagg caaaaatgca
     4621 tataaagcag gaatagattt tgtaggaact actttaagtg gatatactga ggaaagtaaa
     4681 aaacaagatg ggccagatat agatttgatt aaaaaattgg tagaagaagg gatttgtgta
     4741 attgctgagg gaaggattca tactccagaa caagcaaaag agataaaaaa aataggagtt
     4801 gcaggtattg ttgtgggtgg tgctattact cgaccacagg aaattgcatc aagatttgtt
     4861 aaagcattaa gttaggggga gaaataaaat gtttaaaaaa ttttcacaat taggacgcgc
     4921 tttcatgtta ccaattgcta tattaccagt agccggatta ctattgggac tgggaggagc
     4981 gttaacaaat gagagtgcta taaatgctta tccattttta gatcaaccgt ggttgcatac
     5041 aattttaagt attatgagtt atgctggaaa tgcagttttt gcaaacctag cactaatttt
     5101 tgcaattggt attgctgtag gacttgcaaa tggagataaa ggaacagctg gtttagctgg
     5161 tggagtagct tatctagtat atactgcaac tattagtggg tttcttgaac ttttttcagc
     5221 aaaagatgca acactagata caggtgtagt gggatcaatt gttgttggtg gtactgttgc
     5281 atttttacac aatcgttata gaaaaattga attaccacaa ttcttaggtt tttttggagg
     5341 atcaagattt attccgattg tttctagttt agctgcaatc attattggtt ctttctttta
     5401 tttaatttgg ccaccaattc aaaatggttt agtagttgct ggtgaacaaa ttgcccaaat
     5461 gggtagttta ggaacttttt tatatggttt tttattgcgt ttaacaggtg ctgttggact
     5521 acaccacacc atttaccctt tgttctggta tacatcttta ggtggtaccg aaactgtagc
     5581 tggatcaaca attgctggag cccaaaatat tttctttgcc caactagctg atccaaacca
     5641 cactggtctg tttacttatg gtactagatt ttttgcagga agatttgcaa caatgatgtt
     5701 tggattacca gcagcatgtt atgcaatgta tcgtgcaatt cctaagaaaa atcgtaaaaa
     5761 gaatggtggg ctttatttta gcggggccct tacatcattt ttaactggaa ttactgaacc
     5821 agttgaatat atgtttttat ttgttgctcc ttggctttat gtgattcatg cattcttaga
     5881 tggattatct ttttattttg ctgatatctt aaatattcgt gtagggaatt ccttttcagg
     5941 tggtttgatc gactttttat tattcggtgt acttcaaggg aatgacaaaa ctaattggtt
     6001 aaaattaatt ccttttggta ttgcatgggc atttatatat ttctttgttt ttacattctt
     6061 tatcaaaaaa tttaaggtag ctattcctgg aatggaagaa gaatcagaag ttcattctga
     6121 aaaagtaata aacaaaaata catcattaca tgaagagtct cttatcatta ttgaagctct
     6181 tggtggaaaa gaaaatattg agtctgttga agcttgtgca actcgtttgc gtgttgctgt
     6241 tacgaatggg gagattattc aaaaacaaac aataaaagac actggtgcaa ctgctgtctt
     6301 cgaagtgaag ggtggcatcc aagctgtttt tggagggaaa gctgacttat taagtcaaga
     6361 aatcaatcaa atattaggaa aagataatta ggaggttttt aatatgtttg aaattttcaa
     6421 aaagaaaaaa caagaaactt tttatgcacc ttgttccgga aaaatcattt ccatcacaga
     6481 agtaaatgat cctgtctttt cacaaaaatt actaggtgat ggttttgccg tcatacctga
     6541 tggttcaaaa atttatgctc cattggctgg tcgagtaaca aatatatttc ctactaaaca
     6601 tgctattaat ataaaaagca actcaggcat tgattatttg attcatattg gaattgatac
     6661 agttgaatta caaggagagc ctttttcgat ttgtgttcaa gaaaatgatg aggttactcc
     6721 tgaaacctcg ttggttgatg tggattttga caaaataata aaatctggta aagatacttc
     6781 tgttatagtt gtttttccgg agaaaaatca gttcaatgat ttaataatag ataatggtcg
     6841 aattgctcat gggaattcat gtggaaaaat cactttttaa aaaagtgtat ataaaaagct
     6901 ataaaataag ctaaatagga tatttgtcaa atgaagttgg tgaacagaaa gaactgtttt
     6961 ctttctgttc atttttttat tttattagta atgattcccc atgccaaatc atgatccgta
     7021 atttaaagtt ataaaagctt cgaaatccat tggcatttcg ttggaatacc ttgatatgat
     7081 tattcaaata taaaatatag agagaagaga gagagggaag atatttataa agggcaaagt
     7141 tatgtttact caacttagga aatagaaata gatcaattta tattctcttg tcaatcgaat
     7201 atgaacctta gcactatata ccaaatatag taaaatgctg aaagatgt
//
LOCUS       DAZKOK010000130         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000130 DAZKOK010000000
VERSION     DAZKOK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.133"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG90W_002487"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG90W_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0478807.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG90W_002488"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG90W_002488"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0478808.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG90W_002489"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG90W_002489"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0478809.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG90W_002490"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG90W_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0478810.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG90W_002491"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG90W_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0478811.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG90W_002492"
     CDS             complement(4433..5290)
                     /locus_tag="ACG90W_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0478812.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG90W_002493"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG90W_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0478813.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAZKOK010000131         7041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000131 DAZKOK010000000
VERSION     DAZKOK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.134"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(500..3205)
                     /locus_tag="ACG90W_002494"
     CDS             complement(500..3205)
                     /locus_tag="ACG90W_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0478814.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3511..4458)
                     /locus_tag="ACG90W_002495"
     CDS             complement(3511..4458)
                     /locus_tag="ACG90W_002495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0478815.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4648..4917
                     /gene="rpsN"
                     /locus_tag="ACG90W_002496"
     CDS             4648..4917
                     /gene="rpsN"
                     /locus_tag="ACG90W_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0478816.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5093..5455)
                     /locus_tag="ACG90W_002497"
     CDS             complement(5093..5455)
                     /locus_tag="ACG90W_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0478817.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5466..6587)
                     /gene="alr"
                     /locus_tag="ACG90W_002498"
     CDS             complement(5466..6587)
                     /gene="alr"
                     /locus_tag="ACG90W_002498"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0478818.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6603..6953)
                     /gene="acpS"
                     /locus_tag="ACG90W_002499"
     CDS             complement(6603..6953)
                     /gene="acpS"
                     /locus_tag="ACG90W_002499"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0478819.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         1883 a         1506 c         1282 g         2370 t
ORIGIN      
        1 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
       61 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
      121 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
      181 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
      241 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
      301 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
      361 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
      421 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
      481 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
      541 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
      601 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
      661 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
      721 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
      781 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
      841 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
      901 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
      961 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
     1021 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
     1081 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
     1141 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
     1201 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
     1261 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
     1321 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
     1381 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
     1441 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
     1501 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
     1561 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
     1621 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
     1681 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
     1741 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
     1801 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
     1861 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
     1921 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
     1981 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
     2041 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
     2101 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
     2161 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
     2221 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
     2281 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
     2341 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
     2401 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
     2461 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
     2521 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
     2581 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
     2641 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
     2701 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
     2761 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
     2821 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
     2881 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
     2941 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
     3001 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
     3061 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
     3121 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
     3181 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
     3241 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
     3301 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
     3361 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
     3421 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
     3481 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
     3541 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
     3601 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
     3661 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
     3721 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
     3781 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
     3841 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
     3901 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
     3961 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
     4021 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
     4081 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
     4141 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
     4201 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
     4261 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
     4321 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
     4381 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
     4441 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt
     4501 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
     4561 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
     4621 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
     4681 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
     4741 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
     4801 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
     4861 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
     4921 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
     4981 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
     5041 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
     5101 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
     5161 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
     5221 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
     5281 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
     5341 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
     5401 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
     5461 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
     5521 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
     5581 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
     5641 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
     5701 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
     5761 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
     5821 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
     5881 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
     5941 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
     6001 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
     6061 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
     6121 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
     6181 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
     6241 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
     6301 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
     6361 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
     6421 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
     6481 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
     6541 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
     6601 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
     6661 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
     6721 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
     6781 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
     6841 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
     6901 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
     6961 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
     7021 ataagtccac aaataacaaa a
//
LOCUS       DAZKOK010000132         6886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000132 DAZKOK010000000
VERSION     DAZKOK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.135"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG90W_002500"
     CDS             complement(<1..182)
                     /locus_tag="ACG90W_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0478820.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG90W_002501"
     CDS             517..951
                     /locus_tag="ACG90W_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0478821.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG90W_002502"
     CDS             974..1294
                     /locus_tag="ACG90W_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478822.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG90W_002503"
     CDS             1435..1626
                     /locus_tag="ACG90W_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478823.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG90W_002504"
     CDS             complement(1697..1954)
                     /locus_tag="ACG90W_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0478824.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG90W_002505"
     CDS             2112..2375
                     /locus_tag="ACG90W_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478825.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG90W_002506"
     CDS             2399..2587
                     /locus_tag="ACG90W_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478826.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG90W_002507"
     CDS             complement(2670..2855)
                     /locus_tag="ACG90W_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478827.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG90W_002508"
     CDS             3070..3441
                     /locus_tag="ACG90W_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0478828.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG90W_002509"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG90W_002509"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG90W_002510"
     CDS             complement(3709..4137)
                     /locus_tag="ACG90W_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478829.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG90W_002511"
     CDS             complement(4197..4955)
                     /locus_tag="ACG90W_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0478830.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG90W_002512"
     CDS             5250..5447
                     /locus_tag="ACG90W_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478831.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG90W_002513"
     CDS             5553..6035
                     /locus_tag="ACG90W_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478832.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG90W_002514"
     CDS             complement(6093..6689)
                     /locus_tag="ACG90W_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0478833.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1058 c         1353 g         2144 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga attttt
//
LOCUS       DAZKOK010000133         6851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000133 DAZKOK010000000
VERSION     DAZKOK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.136"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            528..722
                     /locus_tag="ACG90W_002515"
     CDS             528..722
                     /locus_tag="ACG90W_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478834.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            913..1162
                     /locus_tag="ACG90W_002516"
                     /pseudo
     CDS             913..1162
                     /locus_tag="ACG90W_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1406..2236)
                     /locus_tag="ACG90W_002517"
     CDS             complement(1406..2236)
                     /locus_tag="ACG90W_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0478835.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2444..3778
                     /locus_tag="ACG90W_002518"
     CDS             2444..3778
                     /locus_tag="ACG90W_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0478836.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3819..4730
                     /locus_tag="ACG90W_002519"
     CDS             3819..4730
                     /locus_tag="ACG90W_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478837.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4792..5580
                     /locus_tag="ACG90W_002520"
     CDS             4792..5580
                     /locus_tag="ACG90W_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478838.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5577..6193
                     /locus_tag="ACG90W_002521"
                     /pseudo
     CDS             5577..6193
                     /locus_tag="ACG90W_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6195..6713
                     /locus_tag="ACG90W_002522"
     CDS             6195..6713
                     /locus_tag="ACG90W_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0478839.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2060 a         1186 c         1373 g         2232 t
ORIGIN      
        1 gttattttat aataaagata tgatgactaa tttagctcaa ctattccagc gtcagctgaa
       61 gatattgcaa ttcccgattc cttaatcatt tatctggatg agacagaaag tgaagttgca
      121 acagatgtgc atgtagttga ggataaaaat gatagtttaa aaattaatag ttcagatact
      181 ttactaagag taagtcctat cttttcagct gttatttaga tgcaagtagc aactaagtta
      241 ggtagtactc ctgcaatgga taggtatttg tgtgttcgat atataagtgg tcgaagtatc
      301 aaggttatgt atactggatg ggagatagaa gagttaaaaa ttggaggtca ccgccacaaa
      361 tggaatttag atttagaggt accttagatt tggtatagtt attaaacaca agatggcctg
      421 gtttgtaggg aaagtcgaag agtatttttc cacttctgcg cccctttaat ttttctagta
      481 atgccgtgaa tttgtgatta attggaaata tgaagaaagg agagtctatg aaaacaacac
      541 tagttctatt ctataaaaag catccttatt ttacacttct gataaatata ttgttggcta
      601 gtgttatcgg gatttctgtt gaatatctaa taaataaaga tttcataggt tcttgttttt
      661 atacggcttt atttcttgga cttcttgaag cattttccat ttataaaaaa tcaaaaaaat
      721 aaaatagatt gccgtaagag atctttttca tttcattttt ttctgaaaac ccgttgtttc
      781 tgtcagtgaa gcagcgggtt tttagattta ttgtctttag acctaattga gcgcgccgag
      841 cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa taaagggaaa tagtttagcc
      901 cgtacaaagt agatgtgtaa ataccacatt gtattcacac caaagtatag aagaaaaata
      961 atttataatc aatatcgagc caatattcag gagatcatca agagattgtg caaatctaaa
     1021 atataaattt gggtatcgac acttttgggt ggaaggatac tatgtgacaa aagtaggaca
     1081 aaatgaagcg accatcaaaa agtacattca agaacaagaa aaacatgata gtgcgttaga
     1141 taagctgagt gtacgagagt aagacgatcc ttttaagggc taaggagtag tacataaacc
     1201 ttttaagagg tgggcaaaac tggcaaaggc aatatggctt gaacataaaa tatgaaagcc
     1261 agcgtcttta ggcgctggcc ggtgatttga gcttatagct ctagagaaaa ccaccctttt
     1321 gaagggtggt tatgattaat gactttagtc agttttgctc acaaagcgtg tggaacaaaa
     1381 aacgggtgtt tctacgggta gatatttatt tcttgaatgg aggtgtccca tacatttttg
     1441 gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt aggttctttc aaccattgat
     1501 attcgccttc gccatccagt gctttgccac tagcccaaga accttcacta ctttggtctc
     1561 cctctgagaa gttatagaag tcataagcaa tgtcttttcg ttctttgtca cgcgggaaag
     1621 tacttgggac taagattgaa ccttcttttt cttctaattc tttcactgca cattcccata
     1681 gattttgatg atagctatct cttgcaagga gaacagacag catatctttt acgccacgat
     1741 catcgatcat ttcgtaaata cgtgctactt gaagtcttcc ttgtgattcg gcatttaaat
     1801 tagctctaaa gtctgctaac aagttgccac tcgcaatgat ataggcaccg ctccatggat
     1861 tgcccacgct gtcagcagga cgaggtccta agccagctac gatcgcgtgc tgtggattca
     1921 ttccagagat aatggcaccg attgttggat ctttttgcat cgctgcttct tgatcttcaa
     1981 caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa catttccacg tggccgattt
     2041 cttctgttgc aatgtccatc agcatatctt tatatttttc ttctcctcgg cagctccagc
     2101 cttggaacag gtactgcata gctacactca tctcgccata ttggccgccg atcagctcct
     2161 gcagatattt cgcgattaat ggattcggac gatctggttt tgcttcaaac tgcaattctt
     2221 tctggtgtct aaacatcaaa attcctccta atctattttt aatacattct cattctgcta
     2281 cgaaagatta aaaaattcaa agaaagcgct cgcgaattat ataaagtatt ggtatcatta
     2341 ataatttttt tagaatcata ccttataaat tgttattttc agaacaaaat gatatcgtta
     2401 tattaaaacc ggcatcggtt acattaaaat ttggaggtaa aatatgaaaa aattattgtt
     2461 tacagggttg ctggcaagtt cagcattgtt tttattagga ggatgcggat caagtaatgc
     2521 aagcgatgcc agttcaagtg cagcaaattc ttctgaaaaa acaagtgatg gaagaaaaaa
     2581 tattgatttc tggtcctttt ggggatccgg tgcacgaaaa gaagctatcg aagaaatcat
     2641 tgaggatttc aatcaatcac aaaacgagat ccatgtaaat tataagtatc agccatgggg
     2701 tgacatctgg actaaatctt tatctgcaat aacagctgga aatgcacccg atgttattgt
     2761 acaggacata aattccgttg cccaaagagc agaagcacag caagcaacta atctctccaa
     2821 atatgtagaa aaagacactt ctaaggattt ttatccgcag ctttgggata cagtagaata
     2881 tgaaggtgat ccttatgcga tcccattcaa tacagataca caagtcattt tttataataa
     2941 aaagatattt aaagaagctg gaatcagtga acaagatcta ccgacaactt gggatgaatt
     3001 agaagaaaca gcacgtaaac tagataaaaa agaaggaaaa gaatttacac gcatcggttt
     3061 ttatccatta tggaatttag gggctgatgt atgggcgcta aacgcagata acgggactag
     3121 ttggtttgac aaagatgaaa aagtcaagat tgatactaaa aataaagtgg aagccttgga
     3181 gtggatccaa gactggcaag attattatgg gaaaaatacg atcaataaat tagaagccga
     3241 atttgggtct ggtgtatctg atccatttat ttccggatta gtagccatgc gtgcccaaaa
     3301 tatcaattat tactctagtt tgatggaaaa tgcaccggat gattttgatt ttggcgtgat
     3361 gcagctccca gaaaaagaag aaggttcagg ccattggtcg tggggtggcg gattcgtctt
     3421 ggaagtacca tacggagcaa aagatcctga ggcctcttat gaattcatca aatatctttc
     3481 ttctccagaa gtacaagaaa aattcggcga aaaaagtttt gatatcatgg cgagcaagac
     3541 agccaatgag aatttagtga ataatgacaa cctaacggat aaaggaaaaa tgatctatca
     3601 aatggcggac gaaaacttta aaaatacggt gatcacacca gttcctttag atgctccgga
     3661 ttacacgaca ttagtgaatg aacagattga ccagattctt ttaggaacaa aatcaccaaa
     3721 agaaggattg gcagatgcac aaaaagcagt agaaaactta gtcaagcaaa atcaataatc
     3781 catttttgac tgtctaggta cagaggaggc aagaaaattt gaagcgttta tctatccgaa
     3841 agaaaaaaga agcaagatgg gcctatttat ttatctcgcc ttttattatc gggttcttgg
     3901 tatttatgct agggcctatg cttttttcag ttattggtag tttcaccgat tataatttga
     3961 catccaagat gaatttcatt ggtctagcaa atttcaaaag aatgttcgtt cacgatgatt
     4021 tattttggaa atcgttatac aacaccgttt atttcgtcat cttcaatgta ccgctgacga
     4081 cgattttcag tgtgttttta gccacagtac tcaatcaaaa aatcaaagga atcagtgtat
     4141 tccgtacagc tttctattta ccggcagtct tatcaggagt agcagtctac atcttatgga
     4201 tgcagctgct ttctccatct gcaggattga tcaacacatt tctgggctgg ttccatatcc
     4261 ctggaccagc ttggctgttt gatccacaat ggacgaaacc tgcgttgatt ttgatgaaaa
     4321 tatggagttc tggaggagca atgctacttt acttagctac attacaaaat gttcctagat
     4381 ctttatatga atcagcagaa atcgatggag caggcgtttg gggaaagttc aaaagtatta
     4441 ctttgccatt aatcactcct attatttttt acgatgtgat cacctctacg atcggttctt
     4501 tccaaatttt tcaagaagct tatgtcatga cgaaaaatgg aacaggcgga cctgcaaatt
     4561 ctttgctttt ttataatctg catatgtgga acaaggcgtt cgtagctttt gatatgggtt
     4621 atgcgatggc tatgtcaatg atcctgtttc tgatcgtgtt ggttctgacg tttgtcaatt
     4681 tgaagcttgc acctaagtgg gtctattatt ctggaggtga cggcaaatga tggattattt
     4741 caaaaagacc agtgtgaatc ggcaaacaaa aaaatactaa gttatgtcct catgacagta
     4801 ataggaatca ttttgatcat tccattgctt tggatggtgt ttacttcatt gaaaccaatg
     4861 gaagaaatcg ttcgttatcc tccgaccttt ttcccagaaa aaatcgtatg ggaaaactat
     4921 ctggatacga ttgccgcctt tcctttttgg cgctatgcaa gaaatacatt attcatcact
     4981 gtgctagtag tgatcggtaa tgtcctttca aattctttta ttgcttatgg atttgcgaag
     5041 cttgattttc ctgggaaaaa gctgatgttt gctttggttc tttctacgat gatgatcccg
     5101 gggtttgtaa cgatgatccc tcaatatgta ttattctcca agatcggctg ggtaggtact
     5161 tatttgcccc tgatcgttcc atcgttcttc ggcaatgcgt tcaatatatt tctgatgcgt
     5221 caattttact tatcgatcaa taatgaattg atcgaagctg ctgaaatcga cggagcgaat
     5281 catttgtata tttggagtca tttgatgctt ccattgacga aacctgcctt gattaccatt
     5341 gcgatcaatt ccttcaacgc agcttggaat gatttcttag gtcctctgtt atatatccaa
     5401 gatcaagaaa aatacacgtt acagattggc ttgcaagttt ttcagaacca agcaacgaca
     5461 caatggaatt atctgatggc aggagcaaca cttgtcttga tcccgacgat tttgctgttc
     5521 ttttttgcac aacgttattt cattgaaggg atggatttga caggaggttc aaaaggatga
     5581 tgaatcgatc gattggtaaa aaagccaatg attatttctt agaaatcatt gaatggattc
     5641 cacgtcttat ccatttacag ctactatggt tcctgtgcgt cttgcctgtt ttcacattag
     5701 gaactgcaag tcgaacagtt ctgtatatga tttatcatta caaaaaaatg aagagaaaaa
     5761 gctcggttca ctattctgga aaaagtttcg tgagaacttc tctctttatg gaaagcaaga
     5821 tagtttagca agtgtttatt ttcttcttct attgatcgat tatcagattt tccgctattg
     5881 gggtggggga attggctata cattgatgta tactacgttg tttttgcttc tattaagcgc
     5941 gcttctcttt agttacaaaa tcttgcttca actggaacaa aaagaagctt cttggattac
     6001 tgcatttttc ttgtttttta atgatttcaa aagcgcgctt ttctatttgg caggcacgtt
     6061 gatccttctt gtcgcattct ggtttgcagg accaatctat tttgctttat taggtttcag
     6121 tttcttgttt tattttcaag tcctgctttt tatccatcgt acgcaagaaa aggttcttaa
     6181 aaaggaggaa taacatgttg cgtttgattg ctaaaatcga tgatagtatt atcaacaagt
     6241 tcgccaagca acggaaaacg gatagtcgaa aaatcaattt tttgaaattt atcagtaata
     6301 tctctaatgg tggttttatt tggttgctcc ttgctgctgt tttagtcttg atacctgata
     6361 ctagagaata tggtctagcc attcttatcg gcagtttgat ctattttttt gttggtttgc
     6421 tttttatcaa aaaaacgaca agacggaaaa gaccttatga aaaaaatcgt cggcttgtcc
     6481 tttacacaag taaaccgaag acttcttctt ttccttcagg acatacagcg ctagcctttt
     6541 tatcagtagc tgcgttgttc gaattaagtc cagttgcagg aatattagcc tttttatttg
     6601 cttgtttgat cgcttattcg aggatttttt tacttttaca ttatcctact gacattttag
     6661 gaggagctat tttaggaagt gttattggca tcctatgcgc tgccgttatc tgaattaatt
     6721 gtacgaataa agcatgagtg cgtaaatgcg catttgtgct ttattttttt gtttcgagga
     6781 aagcgtagtt gtttattcat ttgtcttaat aacttaagtt ttgttaaatg agaacgttat
     6841 aaaattttat a
//
LOCUS       DAZKOK010000134         6840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000134 DAZKOK010000000
VERSION     DAZKOK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.137"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..624)
                     /locus_tag="ACG90W_002523"
     CDS             complement(166..624)
                     /locus_tag="ACG90W_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0478840.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(637..1968)
                     /locus_tag="ACG90W_002524"
     CDS             complement(637..1968)
                     /locus_tag="ACG90W_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0478841.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1979..3421)
                     /locus_tag="ACG90W_002525"
     CDS             complement(1979..3421)
                     /locus_tag="ACG90W_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0478842.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3408..4172)
                     /locus_tag="ACG90W_002526"
     CDS             complement(3408..4172)
                     /locus_tag="ACG90W_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0478843.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4276..5151)
                     /locus_tag="ACG90W_002527"
     CDS             complement(4276..5151)
                     /locus_tag="ACG90W_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0478844.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5406..5490)
                     /locus_tag="ACG90W_002528"
     tRNA            complement(5406..5490)
                     /locus_tag="ACG90W_002528"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5454..5456),aa:Leu,seq:caa)
     gene            complement(5508..5579)
                     /locus_tag="ACG90W_002529"
     tRNA            complement(5508..5579)
                     /locus_tag="ACG90W_002529"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5545..5547),aa:Gln,seq:ttg)
     gene            complement(5589..5661)
                     /locus_tag="ACG90W_002530"
     tRNA            complement(5589..5661)
                     /locus_tag="ACG90W_002530"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5626..5628),aa:His,seq:gtg)
     gene            complement(5723..5807)
                     /locus_tag="ACG90W_002531"
     tRNA            complement(5723..5807)
                     /locus_tag="ACG90W_002531"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5771..5773),aa:Leu,seq:caa)
     gene            complement(5828..5898)
                     /locus_tag="ACG90W_002532"
     tRNA            complement(5828..5898)
                     /locus_tag="ACG90W_002532"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5864..5866),aa:Cys,seq:gca)
     gene            complement(5944..6015)
                     /locus_tag="ACG90W_002533"
     tRNA            complement(5944..6015)
                     /locus_tag="ACG90W_002533"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5981..5983),aa:Gln,seq:ttg)
     gene            complement(6027..6099)
                     /locus_tag="ACG90W_002534"
     tRNA            complement(6027..6099)
                     /locus_tag="ACG90W_002534"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6064..6066),aa:His,seq:gtg)
     gene            complement(6102..6175)
                     /locus_tag="ACG90W_002535"
     tRNA            complement(6102..6175)
                     /locus_tag="ACG90W_002535"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6141..6143),aa:Trp,seq:cca)
     gene            complement(6182..6262)
                     /locus_tag="ACG90W_002536"
     tRNA            complement(6182..6262)
                     /locus_tag="ACG90W_002536"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6226..6228),aa:Tyr,seq:gta)
     gene            complement(6264..6339)
                     /locus_tag="ACG90W_002537"
     tRNA            complement(6264..6339)
                     /locus_tag="ACG90W_002537"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6304..6306),aa:Phe,seq:gaa)
     gene            complement(6375..6447)
                     /locus_tag="ACG90W_002538"
     tRNA            complement(6375..6447)
                     /locus_tag="ACG90W_002538"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6412..6414),aa:Asp,seq:gtc)
     gene            complement(6457..6529)
                     /locus_tag="ACG90W_002539"
     tRNA            complement(6457..6529)
                     /locus_tag="ACG90W_002539"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6494..6496),aa:Val,seq:tac)
     gene            complement(6545..6616)
                     /locus_tag="ACG90W_002540"
     tRNA            complement(6545..6616)
                     /locus_tag="ACG90W_002540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6581..6583),aa:Glu,seq:ttc)
     gene            complement(6631..6720)
                     /locus_tag="ACG90W_002541"
     tRNA            complement(6631..6720)
                     /locus_tag="ACG90W_002541"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6684..6686),aa:Ser,seq:gga)
     gene            complement(6760..6833)
                     /locus_tag="ACG90W_002542"
     tRNA            complement(6760..6833)
                     /locus_tag="ACG90W_002542"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6797..6799),aa:Asn,seq:gtt)
BASE COUNT         2078 a         1505 c         1109 g         2148 t
ORIGIN      
        1 ttaagaatcc tgaaaataag ttttctaaat cataggagca acaaaaaaat tcgataatta
       61 acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta
      121 aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca
      181 actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg
      241 agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat
      301 agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact
      361 tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact
      421 ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc
      481 atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa
      541 gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat
      601 gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt
      661 tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt
      721 ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac
      781 tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg
      841 ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac
      901 tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg
      961 acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa
     1021 attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt
     1081 atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc
     1141 aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa
     1201 atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt
     1261 aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc
     1321 tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata
     1381 cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga
     1441 aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc
     1501 agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg
     1561 tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc
     1621 gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc
     1681 tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc
     1741 aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat
     1801 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
     1861 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
     1921 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
     1981 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
     2041 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
     2101 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
     2161 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
     2221 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
     2281 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
     2341 cgtaatacaa agcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
     2401 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
     2461 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
     2521 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
     2581 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
     2641 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata
     2701 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
     2761 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
     2821 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc
     2881 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
     2941 agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
     3001 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
     3061 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
     3121 taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
     3181 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
     3241 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg
     3301 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
     3361 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
     3421 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
     3481 aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga
     3541 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
     3601 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
     3661 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
     3721 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt
     3781 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
     3841 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
     3901 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
     3961 ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
     4021 tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat
     4081 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
     4141 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
     4201 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
     4261 agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt
     4321 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
     4381 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
     4441 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
     4501 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
     4561 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
     4621 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
     4681 gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga atctcatttc
     4741 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
     4801 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
     4861 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
     4921 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
     4981 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
     5041 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
     5101 taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt
     5161 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
     5221 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
     5281 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
     5341 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
     5401 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
     5461 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
     5521 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     5581 gaataaaagg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
     5641 taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact
     5701 aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg
     5761 gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt
     5821 agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta
     5881 ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa taaattatag
     5941 aaactggggt agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg
     6001 cttggctata ccccaagaat aaaaagggcg aatgatggga atcgaaccca cgaatgccgg
     6061 agccacaatc cggtgcgtta accacttcgc cacattcgcc atggcagggg cagcaggaat
     6121 tgaacccaca ctaacggttt tggagaccgc tgttctacct ttaaactatg cccctataaa
     6181 atggaggaga gtggattcga accactgaac cctaaggaac ggatttacag tccgtcgcgt
     6241 ttagccactt cgctactcct ccatggtggc gcgggacaga atcgaactgc cgacacacgg
     6301 agcttcaatc cgttgctcta ccaactgagc taccgcgcca tattttaaaa ttattaattg
     6361 aataacaaga acgacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
     6421 gcatgttaac ccctacacca cggaaccagt tcattatgga ggttaacggg atcgaaccgc
     6481 tgaccctctg cttgtaaggc agatgctctc ccagctgagc taaacctcca acattcaatt
     6541 aaaatgaccc gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc
     6601 gcttgaccaa cgggccaggt aaatttgcta cggagagtaa gggattcgaa cccttgagac
     6661 agtttggcac catctacatg atttccaatc atgctccttc ggcctctcgg acaactctcc
     6721 agagtgaaag aagctcaata ctctttctca aaatgagaac tccggcagta ggactcgaac
     6781 ctacgacatc atgattaaca gtcatgcgct actaccaact gagctatgcc ggaataacat
//
LOCUS       DAZKOK010000135         6793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000135 DAZKOK010000000
VERSION     DAZKOK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.138"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1936
                     /locus_tag="ACG90W_002543"
     CDS             <1..1936
                     /locus_tag="ACG90W_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0478845.1"
                     /translation="IEQLTQRWKKRIHWLFVHKSYFLVAAFFYCFTFVQIFVLEPKEG
                     WKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFWYALTATLIINLLLTIST
                     VIKMEMREEPVFPSDLKMLTGLSELLSMVSPVLLIVGGLILLLLLITSIIVQRRLQKQ
                     YSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLFKVNKTFFNQQGAVKDNG
                     PVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQTRSDWLNNQTIVLNLSE
                     SFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGGTANMEWEGLTGLSISNL
                     SASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRRKQVFDKFGFDRFYYLDS
                     PDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQLSTMQNHMPYDDYYSELN
                     YTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKIDKPITFVFYGDHLPAIYS
                     GNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNFGAMALEHANIKVTPYYA
                     LLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEKNLTEKQKEILEDYRLIQ
                     YDLVAGNQYSATWATQKIKD"
     gene            complement(1996..2712)
                     /locus_tag="ACG90W_002544"
     CDS             complement(1996..2712)
                     /locus_tag="ACG90W_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0478846.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2939..3481)
                     /locus_tag="ACG90W_002545"
     CDS             complement(2939..3481)
                     /locus_tag="ACG90W_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0478847.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3486..3713)
                     /locus_tag="ACG90W_002546"
     CDS             complement(3486..3713)
                     /locus_tag="ACG90W_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0478848.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3792..5672)
                     /locus_tag="ACG90W_002547"
     CDS             complement(3792..5672)
                     /locus_tag="ACG90W_002547"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0478849.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5861..6475)
                     /locus_tag="ACG90W_002548"
     CDS             complement(5861..6475)
                     /locus_tag="ACG90W_002548"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0478850.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6617..>6793
                     /locus_tag="ACG90W_002549"
     CDS             6617..>6793
                     /locus_tag="ACG90W_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0478851.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT         2050 a         1374 c         1131 g         2238 t
ORIGIN      
        1 gattgaacaa ttaactcagc gttggaaaaa aagaattcat tggctttttg ttcataaaag
       61 ttatttttta gttgcagcat ttttttactg ctttaccttc gtgcagattt tcgttttaga
      121 accaaaagaa ggttggaaag aaactatcca ggttgcctta aaaatattta ctcagcgaca
      181 agcacccatg atcctgacaa tcatcattat tatgggattt ttcttcttac tattgttgat
      241 ttccaatcgt ttttggtacg ccttgactgc taccttgatc attaacttac tgctcactat
      301 ttcgacagta atcaaaatgg aaatgcggga agagcctgtc tttccttcag atttaaaaat
      361 gctgaccgga ttgtcagaac tattatcgat ggttagccca gtcttattaa tcgtgggcgg
      421 attgatcctt ctcctattat tgattactag tattattgta caacgtagac tacaaaaaca
      481 gtattccttg aagattcatt ggaaacgtcg atttatcagt atcgctgtat tattaggatg
      541 tttttcgggt gtctttttca ttaatcacaa aaattcgcct tctttcttgc tgtttaattt
      601 atttaaagta aacaagactt tctttaacca gcaaggcgcc gtaaaagata acggaccagt
      661 cattcaattc ctaaacaata tcgacattaa aatcatggaa gaacctgctg gctattcgaa
      721 agaaaaaatt aatggaataa tgaaaaagta caacacaaag gcagatgaaa tcaaccaaac
      781 aagaagtgac tggttaaaca atcagacgat cgttctcaat ttaagtgaaa gcttcagtga
      841 tcctgcacgt gtccctaatc tgacagtacc tacagaccct attccttaca tcaaaagcat
      901 tataacagaa ggaacaggag gattgatgtt atccgtcggt tatggtggcg gaacagctaa
      961 catggaatgg gaaggactga caggcttgag tatcagtaac ctgtctgctt cgttagttac
     1021 accttatacc cagctagtgg agcgtcagaa aataagtcca aatattaccg atctattcga
     1081 tgaaaaaata gccatccatc cttttacagc tgctctttat cgcagaaaac aagtgtttga
     1141 taaatttggt tttgacaggt tctattattt agacagtccg gataaattaa cttatacgga
     1201 taaaatagaa caaagcccac gcatatcgga tgattcagca tacaaagaaa ccttgaaagc
     1261 tttacaaagc aatacggaaa cgtcacaatt catccaatta tccactatgc aaaaccatat
     1321 gccttatgat gattactata gtgaattgaa ctacactgct gaaggaactg ctgtcattcc
     1381 taatagaaga aaagaattac aaacttatat gcaaggcctt cactataccg atacagcagt
     1441 aaaaaaattc atccaagaaa tcgacaaaat cgataaaccg attacctttg ttttttatgg
     1501 tgaccattta cccgcaatct atagtgggaa tgattcgaaa aaatatggat tagaacagca
     1561 ccagacagat tatttcattt ataacaacaa gtatagtcgt gaacaagcag ctacgttaaa
     1621 taaagaagtt gttgctccta ataactttgg tgctatggct cttgagcatg cgaatatcaa
     1681 agtaactcct tactatgcgt tgttgacaga agtatctaat catctacctg cattaacaat
     1741 tgatcctaca gaaagcttgt ctaatcgttt taatggaaaa caggtctttg ttacagagga
     1801 tagtcaaatt atccaagaaa aaaatttaac tgagaaacaa aaagaaattc tagaagatta
     1861 tcgtctgata caatatgatc ttgttgcagg taaccagtac agtgcaacat gggcgacaca
     1921 aaaaataaaa gactaacaaa taaactgagc aaggggttgt gataaaagac atgtcacaac
     1981 cccttgttta tctcattaat ttgcttgtcc ataaacgatt tgattaaagc catttttcaa
     2041 ttcattattc gctgtctcgt tgaagtaaag ataattatcg ggattcccct gattttgttg
     2101 tgtcaccaat acttttggct gattttgctg aaacccgaat agatagacgt gctttttcag
     2161 atatggttct gtctctgcat cggaatagac ggctgtgatt tgatagtctg cttgacctgt
     2221 cccactttct gaccattctg cagaaacagg cactccacca atagccatct tccactcccc
     2281 gttcaaaata gcttgaggga ctttaagacc atataagtcc acactcattt gattcgtata
     2341 acttttgtat tgttgtccca ttgtttggcc ccatttggtc ataaatgcct ctaattgtgc
     2401 ggatttgtct gcattccaca gtgtttgttc aacttgttcg ctgctttctg aagaggaaga
     2461 tgaagcctct acagaagaac ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga
     2521 ctctgacgaa gtagaggtat ctgctgtttc tgtacttgct gcagttgact tgctctctgt
     2581 gctagttgtt gtagtagttg caactgttgc tttcgctgtt tcttttgttg attctttttt
     2641 cgattcattc ccgttggtac aaccagctaa caaaaaagct agaccaattg ttactaaaat
     2701 cctttttttc aattaaaaaa ctcctttcga ttactcccac ttctttattc tatcaaataa
     2761 gcaatgtgat tttattgatt tttcgtattt tctcatcaat ctttcaaatc ttctaaaaac
     2821 tgagaagcac ccgctgatgt tgtctgtaat acatcagcgg gtgcttcttt tattttaatc
     2881 ttcgtgattt tccaatttac ctcacgcttt atatttgtat ttgaagaaac atagactttt
     2941 agtcatcatc gtcttcctca tccaaatctt gtcggacagt ttttccttgg agtctttgta
     3001 cttggtagat gatttcttta ttgaaactta aaagttcaac aagcaattgt tgcaagccat
     3061 atttttcttc tttttcagct aatttgattg cccgtgtgat aaagacattt tggaaatcaa
     3121 aatcttttgc aaagttttcc aacattgttg aggctggtaa atatttgttt tgaccatgtt
     3181 cgtaaatttt tccataacgc atgacttctt ctaaagtaga cggaacgatt tcattttcaa
     3241 tcactaactc atgagagatt ttattgaacc atctcgcttc ttgatcagca tattctgaaa
     3301 aaagggtact taattcttgc gaagatgtac ccaaaacata caattgtgct tggatcaatt
     3361 tcatttcttc taatttaaaa tttgctaata catgccccac catggcgcca gcagttggtt
     3421 tatgatggtc gatatctgct tgttttgttt cttgaacaaa tttttcttcc actatattca
     3481 ttgatttacg cctcttttac ttttgctgat tggacttcat aacctaattt ttccacaact
     3541 gcagctaatt gttcagggtg gacttcatct ggatcaaagt ttgctttgat tttgctcgca
     3601 ttaaacaaaa ctttcgtatt gcttacccca ggctgttgag aaaccgcatg ttcgattttt
     3661 tgcatacagc ttggacaagc tagtgtatct aattgcataa taattttttt catcgtagtt
     3721 cttccttcct ttttcttgat agtcttattc tagattacag cttccataaa aaaattgatt
     3781 cttatcaagt tttacgattt ttcttctttg ctttcgtgtc caaaatacaa taaacgcatc
     3841 gcgttaaaga taacgatcaa gatactagct tcgtgaacga acatcccact agccatgtaa
     3901 ataaacccgg caaataagcc gttgagtaaa aataagacga caccaatggc aatcactaca
     3961 ttttcacggg tatttaaaac agttttctta gccaagcggt aggcatgaac caacgattca
     4021 aaattagatt gcatcaagac aacgtcactc gtttcgatag caacatccgt tcctgatccc
     4081 atagcaatcc cgatatcagc cgcagccaaa gagggagaat cattgattcc atctccaata
     4141 aaagcaacgc gcaatccttg ttcttggtat tttttcacaa attgaacctt ttcgtctggt
     4201 aataattcag catggacttc atcaatgcct aacatatcag ctacataatc agcagtcatc
     4261 tgattatcac ctgtcagcat cactaactgt tttgctcctg cttgtttcaa cttagctaat
     4321 tgttcagcga cttctggtcg aatcacatct gacacaccta tgatttgagt gatttttttg
     4381 tcgattgcta caataatgac cgagctgcct gtttgttgta attgatgtaa atcttgtact
     4441 tgctttggtg tgagagaaag tttgtgagcg gcaatcaatt tggtgttgcc cgcacaaact
     4501 gtatgaccat caatctcagc tgtgattcct tgtcccttga ttgtctgatt atctttgact
     4561 gctaattgtt gaaaatcaat gtctttctcc tgagcataat ggatcacggc gcgccccaga
     4621 ggatgatcgg ataacgtttc taatcgagcc actaaagcta agctgtcccc ttcatcctct
     4681 gtatagttct tgatagtgga tacagcagta gttcctttgg tcaatgtacc agttttatca
     4741 aagacaaaag tatcgatttt ggaaaaagta tccattgctt caccgccttt gactaagatg
     4801 ccccgtttcg ctccattacc gattcctgct acattcgaaa caggcgcacc aatgactaaa
     4861 gcgccaggac aaccaagaac caaaacagtg attgctaaac gaaaatcacg actaatcaga
     4921 ccaacaacca acgcaataag taaaacggtt ggagtataat actgagaaaa tcgatcaatg
     4981 aatttttccg catgggattt ggtatcttgt gcttcttcta ctaattcgat aattttggca
     5041 aaggtcgtat catctccgac cttagttgct tcaatcgtca cgtagccatc tgctaaaatc
     5101 gttccagaga agacttcatc tccactgatt ttgacgactt gtcgtgattc accagtcact
     5161 gcggcttcat ccgcataccc tttcccgtca atgactcgtc cgtccacagg aatagctcca
     5221 ccagttttta ctaagacgat atcgccttct tccacgtcat cgatttccac ttcttcagtc
     5281 gttccatctt cttggacaac actagcagta gatggggcca tctcggtcaa agccttgatt
     5341 gactcccgcg ttttggtcaa tgttttttgt tccagatagc taccaaacaa aaacaagaat
     5401 gtaacgatag ctgactcctt gtattctccg atcataaagg caccaagcac agcaatagta
     5461 acgagtaaat caattgagat aactttgact ttcactgcct gatacgcatg gatcataata
     5521 ggtatgaccg caataataga agctatggct agagcaatgt tatagaacaa gacaaaacca
     5581 gtaaattttc cgataaaacc aaaaataatc aacacagctg ataaggcggt gatatgattt
     5641 cgatgctctt ggatccactt ttgtaatttc atctttctcc ctcttttcct ttcattctta
     5701 cgagactaag tatagatact tatccatcat aaaaaattga ccactatcaa gtttagtcaa
     5761 aaaggcggtt ttcttttggt agaatacact acaaaaaata gttagcaaga agactcacta
     5821 aaagtgatct tgccaactat tgtcgtttct atcgaaattt tcattgaatg attttcctgt
     5881 acagcataca tcggagatct atttctttat ctaagatttt tatcttgcta attttttcct
     5941 ttagtctttc aaatccacgt cgttcataga attgatacgt acagccacta tctgtaaaaa
     6001 ggaatatttc ttttcctttc tctctttttt caaattctgt tagcaacttg gtaccgatgc
     6061 cttttgtttt caaatcagga tttgcagcta aaaaaatgat ctctccatct ggcgaatgct
     6121 tctttaaata tgctgcaagc atttgttcat ttgctgtctc gtaagctcct ttactttctt
     6181 tagcaaatat attttgtaaa aaatcgaata tcttaacata tatttgctgt ccaaaagaac
     6241 gatgtttctt tttttctcct tttatctccg ccagcaaaac acctgccaac tcgtcctctg
     6301 catacaaagc aagaatttga gttgctcgat tcaactccat atacaaaaaa tatcttccgt
     6361 aaagattcag cagaagttca ttttccatat atctctcaaa gttcattcct tcaacagcaa
     6421 aatagatagc ttttttgtaa tctttctttt ctaagggttt aaaagttagt ttcatcattt
     6481 cgcctcctca acttcaatga ttgtgttata cttacattat aatagacaca gtgtctacta
     6541 tctataatca atcatctata agtttgtcga ttaagcgaca gctttgcttt gatatgtcgg
     6601 ataaggagat aggtaaatga aaaagacaga tagacgcgtc aaaaaaacag aaaaagcttt
     6661 ggctgaaaca ctatccacat tattagtaaa taaaaagatt caagcaatca ctatacgcga
     6721 actaacagaa acggcagatg tacatcgttc gactttttat acacattata aggatatcta
     6781 tgatctatat gat
//
LOCUS       DAZKOK010000136         6735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000136 DAZKOK010000000
VERSION     DAZKOK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.139"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..898
                     /locus_tag="ACG90W_002550"
     CDS             8..898
                     /locus_tag="ACG90W_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0478852.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(971..1795)
                     /gene="nadE"
                     /locus_tag="ACG90W_002551"
     CDS             complement(971..1795)
                     /gene="nadE"
                     /locus_tag="ACG90W_002551"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0478853.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1807..3279)
                     /locus_tag="ACG90W_002552"
     CDS             complement(1807..3279)
                     /locus_tag="ACG90W_002552"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0478854.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3916..4278)
                     /locus_tag="ACG90W_002553"
     CDS             complement(3916..4278)
                     /locus_tag="ACG90W_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0478855.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4501..5253
                     /locus_tag="ACG90W_002554"
     CDS             4501..5253
                     /locus_tag="ACG90W_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0478856.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5393..6316)
                     /locus_tag="ACG90W_002555"
     CDS             complement(5393..6316)
                     /locus_tag="ACG90W_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0478857.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6331..6603)
                     /locus_tag="ACG90W_002556"
     CDS             complement(6331..6603)
                     /locus_tag="ACG90W_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478858.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2054 a         1348 c         1152 g         2181 t
ORIGIN      
        1 gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag cattagctag
       61 taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg tcaaagattt
      121 ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa agctagagga
      181 agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga
      241 taaaatcgat attagttttc cagaaaaaat attcaatgct tttgatacga aagaaacatc
      301 gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt tagctacgat
      361 ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc gaaggatgga
      421 tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta attttttaaa
      481 tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg aatttccttt
      541 ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg agctaggaac
      601 atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg attggtatcc
      661 agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac acttgacgaa
      721 tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag agcaagacac
      781 ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct gtactttatt
      841 tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact acagctgata
      901 tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga cggctgtttt
      961 tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt
     1021 gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact tcttttcctt
     1081 ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct gcaatcattg
     1141 gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc ggtgcattca
     1201 atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg
     1261 ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc
     1321 ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata cgttgacgtg
     1381 ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt aatacttgta
     1441 cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc tggataaagt
     1501 caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga cgaacagcga
     1561 taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt agttgcgcca
     1621 aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa gttttaagaa
     1681 acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt tcttcttttg
     1741 gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg ctcatttatt
     1801 ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt ccatgatagc
     1861 cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg gattcaagtc
     1921 tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt tgatttcgtt
     1981 tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta aaactgggcg
     2041 agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga acatgaagat
     2101 agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt cagatttccc
     2161 atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta ctttttctgc
     2221 attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga tcgatactag
     2281 cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg tcccaacacc
     2341 ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt catccaagtc
     2401 attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt cccgaacacg
     2461 cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt tgattttgtc
     2521 acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg tatcataagt
     2581 gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg cttcataatc
     2641 atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta tcccaaatat
     2701 cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg ctctagttcc
     2761 ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata gtggatcttc
     2821 tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga aattgaccat
     2881 attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca cttggatcaa
     2941 tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg ctttaaattc
     3001 aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac gcaagtaagc
     3061 aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt ccaatcctgc
     3121 aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa aaacagcatg
     3181 taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat atagatccgt
     3241 atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact cctcttggca
     3301 agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa tcttccattt
     3361 tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc catctgctaa
     3421 aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt ttattcaatg
     3481 tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa ataagtagat
     3541 tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata accaaatgtc
     3601 tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc cttttttgct
     3661 tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg gaaaggcgaa
     3721 ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa
     3781 aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg cgagacatca
     3841 atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta ccataaagat
     3901 gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca ctacaatatc
     3961 tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca ctcctacatg
     4021 gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt cgatttcttg
     4081 ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat atgtgtctac
     4141 caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag ccgtattccc
     4201 aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac tattcacagg
     4261 tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga tccaatctta
     4321 agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc tttaaagaca
     4381 aaaggattta ccttttatca cttaatagta taacataatt tcatgagaaa aaaatacaat
     4441 tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg aggaaaaata
     4501 atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag aaccttgcgc
     4561 ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg ttatcggatt
     4621 tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac aatggcattc
     4681 aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca gaaagcttta
     4741 atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct cttgacaact
     4801 gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt
     4861 gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca agagattcga
     4921 caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa tatggataaa
     4981 gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct cactctttac
     5041 ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca taagaagtgg
     5101 ttatcttaca gctggcctga atatcagcca aaagcgcata aaggactagg aatgatgtac
     5161 gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa
     5221 gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa caaaaaagta
     5281 cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt atgctaactt
     5341 ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat attcatcgtt
     5401 gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac aattgaaatg
     5461 ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga agctcgctga
     5521 tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag ctttgcaaga
     5581 tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc tctttaaaaa
     5641 agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca agtgattcat
     5701 caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta taacttagcc
     5761 tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc aaataagtaa
     5821 gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt tttcccttga
     5881 tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca tctagtgtat
     5941 tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct tcaagatacc
     6001 ttcgctcttt ttctttctga tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa
     6061 acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg gtataaggca
     6121 gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt gcattaggga
     6181 tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca tctacatatg
     6241 gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc tttgcgatat
     6301 ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt gtaaatagtc
     6361 taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg aaaaactgat
     6421 ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca ttggtatttc
     6481 ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg atcccttgaa
     6541 ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta cataagtatt
     6601 cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc tcatagcaag
     6661 gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa gtctcttatt
     6721 ttaaattgaa tacat
//
LOCUS       DAZKOK010000137         6553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000137 DAZKOK010000000
VERSION     DAZKOK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.140"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..1097
                     /locus_tag="ACG90W_002557"
     CDS             123..1097
                     /locus_tag="ACG90W_002557"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0478859.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1267..2667
                     /locus_tag="ACG90W_002558"
     CDS             1267..2667
                     /locus_tag="ACG90W_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0478860.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2745..3467)
                     /locus_tag="ACG90W_002559"
     CDS             complement(2745..3467)
                     /locus_tag="ACG90W_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0478861.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3546..4052)
                     /locus_tag="ACG90W_002560"
     CDS             complement(3546..4052)
                     /locus_tag="ACG90W_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0478862.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4175..5974
                     /locus_tag="ACG90W_002561"
     CDS             4175..5974
                     /locus_tag="ACG90W_002561"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0478863.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(6042..>6553)
                     /locus_tag="ACG90W_002562"
     CDS             complement(6042..>6553)
                     /locus_tag="ACG90W_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0478864.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         2075 a         1346 c         1258 g         1874 t
ORIGIN      
        1 cttttacata taatacaacc ttgttatctg ttatatttta tagccaaata gcgtgacatg
       61 gggcttgaga aatgttagca taagcatggg aaaaaacaaa tcaatggaag gattgatact
      121 aaatgactgc agctgcagaa aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg
      181 ccgtaggttc tagctatgcc tttgctctag ttactcaaaa tatcgctcaa gaagtaggta
      241 ttgtggatat caatactgct aaaactgaag gagacgcaat tgacttgtct cacgctttgg
      301 catttacttc acctaaaaaa atctattctg ctacttacgc agacgcacat gatgctgact
      361 tagttgttat tacagctggc gcaccacaaa aaccaggtga aactcgttta gacttagtaa
      421 acaaaaacct aaaaatcaac cgcgatgttg tcacacaaat cgttgaatca ggatttaacg
      481 gtatcttttt agttgccgct aacccagtag atattttgac ttactcaact tggaaattct
      541 caggtttccc taaagaacgc gtaatcggtt caggtacctc tcttgactca gctcgtttcc
      601 gtcaagcact tgcggaatta gttgacgttg atgcacgtaa cgtccatgct tacatcttag
      661 gtgaacacgg agattcagaa ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa
      721 tctatgaatg ggtaaaaaac aacccagaaa tcgatgaaga agctatggtt aacctattct
      781 ttggcgtacg tgacgctgct tacacaatta tcgagaaaaa aggcgctact ttctatggta
      841 tcgcagtagc attagcacgt atcactcgtg ccatcttaga tgatgaaaat gctgttcttc
      901 ctttatcagt attcatgaat ggcgaatatg gattgaacga catctacatt ggtgcacctg
      961 ctgttatcaa ccgtcaaggt atccaaaaag taatcgaaat tcctttaagt gacagcgagc
     1021 aagaccgtat ggctgcttct gctaagcaat taaaagaaat tctagatgaa gcttttgcta
     1081 aactagacgc tgaataatct ttgaacatga cggaaaacat ctattcggtg ttttccgttt
     1141 ttttatttta tcacccttat ggtttctaaa ttttttcata aagtagagat aaccttcgtc
     1201 ttttcctcta aattatgcta tcatgagacc gttccaccct aaacttttac ttaaggagtt
     1261 gtcgatatga caagatcttc gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt
     1321 cttggtgttc tcttagtgct gatcagcttt tatacctatc gcagtacata ctacacaaag
     1381 cattttttgc cgaatacaga aataaatgga ataaatgtca gcaatctaac agtcgaaaaa
     1441 gcaaatgaaa aattaaaaga agcttattct gataaaaaac tggctattaa agaagatgga
     1501 aaagtctggc aggaagtacc aaaatccgag ctcggctaca aagatgattt cacttcggag
     1561 ctttctccga tcctaaatga acaaaacgga tggacttggg gaatgactta tgtctcagcg
     1621 gcagaaaaac aagaaataga tcctgtcagt caaaaccacc aaaaactaga cactaccgtt
     1681 cagacattga caactaagct gactgatttg aacaaagacc ggacacctac tactgatgca
     1741 acgatcgaaa aagcaggtga ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc
     1801 gacgtcgatg ctgcggtcaa acaattaaaa agtgcggtaa actctggtaa agatacaatt
     1861 gaattgactg aattcaaaga aaaaccaaaa gtaacatctg aagacagctc actgaaagaa
     1921 caactcgcat ccatgaatgc tatcgcaaat gtaaaagcta cttacagtat aaacggtgag
     1981 actttccaga tcccatcaag tgatattatg agttggctga cctacaatga tggaaaagtc
     2041 gatttagata cagaacaagt acgtcaatat gtaacagatt taggcacaaa gtataacaca
     2101 agtacgaatg acacaaaatt taaaagtaca aaaagaggag aagttacggt cccagttggc
     2161 acttacagtt ggacgatcca aacagacagt gagacagaag cattgaaaaa agcgattctg
     2221 gcaggccaag attttactcg ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca
     2281 ttgatcgaag atacgtatat cgaagttgat ctggagaacc agcatatgtg gtattacaaa
     2341 gacggaaaag tggcccttga aacagatatc gtttccggaa agccaactac acctacacct
     2401 gctggagtct tctatgtttg gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac
     2461 gatggaacac cttacgagag tccggtaaac tactggatgc cgattgactg gacaggtgtt
     2521 ggtatccacg attcagactg gcaacctgaa tacggtggag acttatggaa aacccgcggt
     2581 tcacacggct gcatcaacac cccaccaagt gtaatgaagg aattattcgg gatggtcgaa
     2641 aaaggaacgc ctgtattggt cttctaacaa aaataatata taaaaagaaa gcacccgtac
     2701 gattggtaca ggtgctttct ttttattatc taacaatgtg ctacttacat ttcagcagca
     2761 gtaaagactt ctgaaacatc atcatcgtct tctagcttat ctactagacg ttccaattgc
     2821 tctttttgtt cctcatttaa atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc
     2881 gccaaagtga actctttttc caactcatct cgtacagcag caaaatcttc tggtgcagta
     2941 taaatctcga atacttcagg agaagtttgc agatcttctg cccccgcctc taatacatcc
     3001 tcaaacatcg tctcttcatc aacagtcaac ccatcgcgtt caatagcgat atatcctttg
     3061 cggtcaaaca agtaactgac tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa
     3121 gctactcgga catttgtcgc tgtccgatta cggttatctg tcaatgcatg gactaagatt
     3181 cctactccac ccggaccata tccttcatac gtgatttcat cgtaattcgt ttcatctacg
     3241 gcactcgtag cctttttaat cgcccgttcc acattgtcgt taggcatatt tgcagctttt
     3301 gctttgtcca caacaagccg caaagctgga ttcatagatg gatcaggacc gcctgcttta
     3361 gctgccatat aaatttcacg agaaagtttc tgaaagactt tccctcgttt tgcatcttgg
     3421 gcattttttc ttccttggat attatgccat ttactatgtc ctgacatgat ttttcctccc
     3481 ttgttctttt gagaatacac ttctattcta gtactatttc acaaaaaaac aaagccacag
     3541 ccaatctata aacgcttctt gaatgccgca aaaagcacac ataatatcac agcagtcaga
     3601 gcaccgatcg aatccaacat gacatcttgg aacataggtg ttcgtccacc ggtcatcatc
     3661 tgatggaatt cgtccaaacc tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc
     3721 agccaccaaa tttttagctt ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa
     3781 taggtaccga aatgtgcgcc cttccttaaa aagaactcta aaaatttggc gtagcccaga
     3841 tggttgatac tcaccatact tccaccataa ttaaaacgta tacccgctaa tgtgtcttta
     3901 aatggctgat taggcagcca gctttctaat cgcgaaactt gtgattgctg tccgtaagtt
     3961 tgagaagaac tgtaaaataa gattataacg actatcagtg cgattactaa ataaatattt
     4021 ccgtttttca tttgtttttt tgaagaattc ataagtcacc tctgtatttt atcttagact
     4081 ttttttgaag aaacaacaat cattttcata gagacttctt ccataaaaaa gtgctatact
     4141 taattttgta ttacaatatg aagggagttt tttcatgagt tattcattaa attggaatct
     4201 agattctatt ttttccggag gcagtcattc tgatgctttg aaccaacgga tgaagcaatt
     4261 ggaagaccaa atgaacgagt attatcaccg cgtaacaaaa tggtcaccta gttctgacaa
     4321 tgcagagcag ttaaacgcta ttcttcaatt gcaagaaacg attacgaatg gttttaccca
     4381 atgcagcagc tatattactg cattgctttc tgccaatgtc aatgattcag atgcaaaggt
     4441 cttatcaggc aagctttacg caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc
     4501 aaagaaattt gcagaaatat ctgacaatga ttggaatcag atgctttctc aatcttcgtt
     4561 tgaaacaatt gcttttcgtt taaacgagat tcgtcgtgat ggctcacaat tattatcaga
     4621 agctgaagaa aacattataa atacgctttc tttagatggt ctaaatgctt ggagcagtca
     4681 ttatgatacg attgtagcca gcatttcgat tcccttcgaa caagatggac aagtagtcga
     4741 attatctgct ggacaagcct tcaataagat gatgggcgat ccagatccaa aagtcagaga
     4801 aacacttttc gcagcatggg aagaagcctg gaaagaaaag gcacccctct tcacggatac
     4861 tttgaatcat ttagatggtt ttagactttc tgactatgaa cttcatggtg tcacagattt
     4921 tctacaaaaa ccattagagt acaatcggtt gaagaaagaa acattgtctg tcatgtggga
     4981 tacaatccag aaaaacaaac aaccccttgt taattacttg actagaaaag ctaatctttt
     5041 cggtaaagaa aaaatggaat ggcaagatca agatgcacct attattttag gcgatttgaa
     5101 agaaaaaacc tttagttttg acgaagcagc agcatttatt atcgaaaact ttaacaaatt
     5161 cagtccaaaa atggctaaat ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga
     5221 agatcgtcct gggaaacgtc ctggtggtta ttgtactgag ttaccagaaa caaaagaatc
     5281 aaggatcttt atgacctaca gtaattctgt taatgaggtt gcgacacttg ctcatgaact
     5341 aggacatgct ttccatagca gcacgatgtg ggatttacct tctttaaatc gcgaatatgc
     5401 catgaatgtc gcagaaacag caagtacatt cgcagaactg atcgtagccg atgctacatt
     5461 aaaagtcgca aaaacaaaag aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc
     5521 tttagctatg ttcatgaata tccattcccg attcattttt gaaaatcgat tctatgaagc
     5581 acggcaagaa ggattagtct cagaagataa gatcacagag atgatggttg aggcgcaaaa
     5641 agaaggctat caaggagctt tagctactta ccatccatat ttctgggctg caaaattgca
     5701 tttctttatt gatgacgtac cgttttataa cttcccatac acatttggct acctctttag
     5761 tttgggcatc tatgcttacg ccaaccaaaa gggagcaaat tttgaagaag aatatatcca
     5821 tttactacgc gatactgcct caatgacgac ggaagatctt gcgaagaaac atttgaatgt
     5881 cgatcttacc caaccagatt tttggcaagc aggtatcgac atggtgactg aagatatcca
     5941 aacgttcatg gaattgacag aagaatttat ctgatttcta ttttatcaca ataaaaaaag
     6001 agttagtcca gaatgggcta actctttttg attaatcatt cttacatgct gacagcaaag
     6061 tcaggggcaa accattgaac tgaaccaact tttacgcttt cgcctggttg aggagcatgg
     6121 atatattgtc ctccgcctaa agcaatcgct acgtggtaag ttccgccttg tgacccccag
     6181 aataacaagt ctccagcttt tgcttgagaa actgaaattt ttgttccagc tgattcttga
     6241 ggaactgtcc aaccaccgat gtcacgacca gttacttgca tgtaaacata gcgtgtgaac
     6301 cctgagcagt caaagccact tggatcttta ccgccccaaa catatggagt accaatatat
     6361 ttgtaagctt ctgctacgat tgctgcacca ttcacacttc cagaaggagc tggagcaggt
     6421 gttacaggtt ttggttgttc aggtgttggt gttggcgttg gagccggagt actacttcct
     6481 gtgccgtttc ctgtatcaac actttgatct gttgatggtg ttgtaggcgc aggtgttgtt
     6541 gattcttctg ttg
//
LOCUS       DAZKOK010000138         6318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000138 DAZKOK010000000
VERSION     DAZKOK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.141"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..1228)
                     /locus_tag="ACG90W_002563"
     CDS             complement(98..1228)
                     /locus_tag="ACG90W_002563"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0478865.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACG90W_002564"
     CDS             complement(1877..3079)
                     /locus_tag="ACG90W_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0478866.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACG90W_002565"
     CDS             complement(3139..3342)
                     /locus_tag="ACG90W_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0478867.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="ACG90W_002566"
     CDS             complement(3484..4551)
                     /locus_tag="ACG90W_002566"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0478868.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACG90W_002567"
     CDS             complement(4609..4716)
                     /locus_tag="ACG90W_002567"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478869.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACG90W_002568"
     CDS             4879..5691
                     /locus_tag="ACG90W_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478870.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6065..6256)
                     /locus_tag="ACG90W_002569"
     CDS             complement(6065..6256)
                     /locus_tag="ACG90W_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478871.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1876 a         1299 c          996 g         2147 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaat
//
LOCUS       DAZKOK010000139         6066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000139 DAZKOK010000000
VERSION     DAZKOK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.142"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG90W_002570"
     CDS             complement(254..541)
                     /locus_tag="ACG90W_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0478872.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG90W_002571"
     CDS             complement(561..785)
                     /locus_tag="ACG90W_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0478873.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG90W_002572"
     CDS             complement(1240..1410)
                     /locus_tag="ACG90W_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478874.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG90W_002573"
     CDS             complement(1425..1958)
                     /locus_tag="ACG90W_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0478875.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG90W_002574"
     CDS             complement(1979..2923)
                     /locus_tag="ACG90W_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0478876.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG90W_002575"
     CDS             3560..3646
                     /locus_tag="ACG90W_002575"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0478877.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG90W_002576"
     CDS             complement(3767..4456)
                     /locus_tag="ACG90W_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478878.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACG90W_002577"
     CDS             complement(4463..4741)
                     /locus_tag="ACG90W_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478879.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACG90W_002578"
     CDS             complement(4701..5615)
                     /locus_tag="ACG90W_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0478880.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG90W_002579"
     CDS             complement(5597..5977)
                     /locus_tag="ACG90W_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0478881.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DAZKOK010000140         5943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000140 DAZKOK010000000
VERSION     DAZKOK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.143"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1031)
                     /locus_tag="ACG90W_002580"
     CDS             complement(33..1031)
                     /locus_tag="ACG90W_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478882.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            1250..2470
                     /gene="sstT"
                     /locus_tag="ACG90W_002581"
     CDS             1250..2470
                     /gene="sstT"
                     /locus_tag="ACG90W_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0478883.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            2617..3252
                     /locus_tag="ACG90W_002582"
     CDS             2617..3252
                     /locus_tag="ACG90W_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0478884.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            3443..4183
                     /gene="truA"
                     /locus_tag="ACG90W_002583"
     CDS             3443..4183
                     /gene="truA"
                     /locus_tag="ACG90W_002583"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0478885.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(4328..5941)
                     /locus_tag="ACG90W_002584"
     CDS             complement(4328..5941)
                     /locus_tag="ACG90W_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478886.1"
                     /translation="MDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNV
                     WSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVS
                     YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYI
                     RDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVL
                     TFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLG
                     LIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL
                     FIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAV
                     AIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNT
                     VLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYL
                     SIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2017 a          898 c         1120 g         1908 t
ORIGIN      
        1 aagataacta ggagggacac tgctaataaa tatcaaataa aaaactgccc gatatttatc
       61 agccatgcct tccctgagtt tggaaattca cgtttcataa atgtagatac gcgataagaa
      121 actaaaggga aattacatat aacttgagtg acaatcaaaa atacagcaag aaaatttcct
      181 cttcctttta tacctaattt atcagatagc atttctaaaa ataaaaacga agagaaagta
      241 taatacattg cgaagagaga atatggttta gaaaacctca gcgctgtatc tgtatttcct
      301 ctgatgatga gtgaaatttg tgtcatcaaa ataatcaaac cgaataaaac aatcaagctg
      361 ataaatactt gaggaagctt atagcggacc ttttctttcc agttccattc tttcaaacca
      421 taacccatta aaaataaata aaaaatccat aaaatactct gtccattctg taatgcaaac
      481 gggtctttat taaatagagt agagcagcca actaatccaa ttgttaaaaa taatagtaga
      541 tatttaattg ttgttttcga taatttattt aaaaatccaa ctgtataagg aatactacaa
      601 aaaagcaaag tacaagaaac agcataagta aagtaattct gactgatagg tgttaggata
      661 agccaataat cacgaatatt caagctgcca gcttgtacgt aaaaaaagat attgcaaact
      721 agtgtggcta ttaggaatat aaaggcaaaa cgacttaaat aatgtaaaac ttttaaacgg
      781 gcgatttttt gatattggta acccatgtaa agtgggatca aatttgcacc aataacaacg
      841 gaagtttgta aaaatgtata gatgtagaat cgaccagggt ttgttgcata tgtaggaata
      901 gtgactgctg aaatcaatat acgctgtaaa accatcatta tacaagcgac aaagattgtc
      961 aggaagccat aactctcctt ctttattttt ttcaagaata aacacctcac aaaaaaattg
     1021 gacaaaaaca taagaaaacg tttcctgata agttgcccat taataacatc ctaacttaga
     1081 atatctcaaa aaacaacgtg tagacagaaa aagtacaaaa ttagcaagaa atggttgttt
     1141 gtattgttag aatttttaga aaattatacc tttagtttct ttgcataaaa tgatacaatg
     1201 attgttaaaa ttgtgaaagg gtggtcattt gttagagaag tttttggata tgtcgttagt
     1261 tcaacgtatt gttgttggta ttttagtcgg aatattgttt ggtatctttt tccccacatg
     1321 gacatttatt tcaatattgg gaacgttgtt tgtcagttgc ttgaaggcaa tcgcacctat
     1381 tctggttttt ttcttgacga tggcgtcaat tgccaagcat aaaattggga ataagacatt
     1441 cgtgaagcct attttgatct tatatttagt gggaacattg ctttctgcac ttgtagcagt
     1501 tattgtaagt tcaattttta ctgttcctat tactttacaa gaaacagtat ctgaaaaagc
     1561 accgcaaagt ttggaaagtg ttttatctac tatgttgacg aatgtcacac aaaatcctgt
     1621 tcaatctctt attgatgcga attaccttgg cgtcttattc tgggctgtat tgttgggact
     1681 agcttttcga gcacgttcag aatcgacaaa agagttggtg gatcagatat ctatagcgtt
     1741 gtcacaagtc gtacaagtga ttattgcatt tgctccgata gggattttag gattggttta
     1801 tcaatcaatt gctacgactg gaatcgctgg attggcagag tatctgcagc ttttacttgt
     1861 tttgatcggt acgatgctgt ttgtcgcatt agttgtgtac ccattcctca cgtttttgtt
     1921 tattaaagaa aatccctatc cattgatttt cttttgtttg aaagaaagcg cacttcccgc
     1981 tttctttacc cgaagttctg ccgcaaatat cccgataaac atgatgttgg ctgaaagatt
     2041 gaaattgaca aaagagtctt attccatatc gatcccgtta ggtgctacga tcaatatggg
     2101 cggtgccgcg attaccattt ccttgatgac gttgactgct gtacattctt tagggattga
     2161 agcacctttt gttttgaaag tccttttatg tattttgtct gctttagctg cttgtggagc
     2221 ttcaggtgtt gcgggcggtt cactgttatt gattccatta gcatgtagtt tgtttggtat
     2281 ctctaatgat gtcgcgatgc aaatcgtcgg tattggattt attatcggtg ttatccagga
     2341 ttcaatggaa actgccttaa attcgtcaag tgatctgttg tttactgcaa taggagagct
     2401 aggcgcgcgt aaaagaagtg gggaaaaaat caaattgaaa gattgtttag atggattaag
     2461 tgaacgttga acgttagata tttagtattt ttaaaatgat tcgagttttt aatgagttat
     2521 atagatgttc gtgtaaatag aggtttctgt taaatcaaga taaagtcgtt tataataaca
     2581 attaaaagca taggtaattg ttattggagg cgtatcatgg attttttacg taaatttggt
     2641 tttttctttt tattattagg cgtgctgagt gattttttga ctccgtacgt attaggattg
     2701 tattatcctg aattagatca aatgaagagc gtgatcagtg tatttggaga ggtttccagt
     2761 cctgtaagaa aagcgtttct tatttggtct gtggtttcag ggatcttttt catcctgtca
     2821 ttgcctgcat tgtatgctgc atttgttagt acatctcgtg tgttagctac cctagttaca
     2881 gcagccatcg cgttctatgg tataggtgat tgtatcttta caggtttgtt cagtatcaat
     2941 acccatgaat cttcgtggaa tgtctcgaca tgggtccata atatcggatc aggtttaggt
     3001 tacagtggtt ttttattgtt tccagttctt cttttcttga tctatcgaaa aaatgggcaa
     3061 atggacgtaa gccacttata ccttggactg tctgtgatca gtttgctttt tgcggcgatt
     3121 tatggactgg caagaattcc ttctataaat tcttggccat tactgaataa aataggtttt
     3181 tgccaaagaa tcagtttttt ctttaattat gtgcccattg cctggttgag tatcttgcat
     3241 ctcaaacagt aaaacttgag ccgtcaaatt gaacagtcag cggatacttc atccggtggc
     3301 tgttttttgt ttttctgaaa tatatatttc cacagttttc ttgtatagct tctgtcttag
     3361 agaagcaagg agctatattt tgtgctacaa tcttaaaaag gtagagagaa gaccaaatgg
     3421 aagacagagg agccactaaa atatgagaaa catcaagttg acaatcgaat atgacggaaa
     3481 aagatattca ggatggcaaa gacttggaga tgacgataaa accattcaag gaaaaatcga
     3541 aaaagtattg catcagatga caaatgaaga gatagaaatt attggatcag gaagaacaga
     3601 tgctggcacc catgcgcgag gacaagtagc aaattttaaa acaaacacag aaattggact
     3661 ggcagaaatg atcgacttta tgaatcgtta ccttccgcgg gatattgtca tcaaaagaat
     3721 cgaagaaatg cctgagcgct tccattcaag atataatgcg gtgggcaaaa aatacagcta
     3781 ttatgtttgg aacaacgtga ttccttcagc atttgagcga aaccacagct tttatttccc
     3841 tcaagagttg gatatggaca agctgaatgc agcttgcgaa aaattagtgg gtacacatga
     3901 tttcattggt ttttctgcat tgaaaaaatc aaaaaaatcc acagtgcgta cgattgatga
     3961 attatctatc gaacgaaagg gagaaatgct tcattttact tttgtcggag acggattctt
     4021 atataaaatg gtgcggatta tcatggggac attactagaa atcggcacag gtgccatgcc
     4081 gatagaaaca atcgatgaaa tatttgaaag caaagtgcgg caacatgcag gagaaaccgt
     4141 ccctgcacaa ggtctgtttt tggatgaagt ttattatcga taaaaatact ttgataatgg
     4201 atgaaacgca tgtttcacag tataaatatc gatgttaaat aatgtatgaa gcaaattaaa
     4261 gaaatataaa aaaagacttt ttagcctgtt tatgtgtaca gctaaaaagt ctttcttaaa
     4321 taaaaatcta gtgagaatgt tgtacttctc ttctcggttg tttttgttta gaaaaagtaa
     4381 atcgataaag taaaaataaa atcaaagtaa cgatagataa atatcttgaa gcaattgcta
     4441 gttttgtgta ctgataagta ataacgactt tatttatacc tggaggaata gttaattcag
     4501 ttgtgccaaa cttagataat tttgtttgta ctagtttgcc attaatagaa gccacttgcc
     4561 ctttataacg ataaataggt gtcgtcaaga cagtattttt attattcgaa ttatctaatt
     4621 caattgtaag tttgttactc ataaaatgag ttgttggatt tatttcctgg ttacctagta
     4681 aatattgatc attattaatc atatcaggat gatagattga ctctttgttt gcataatcag
     4741 tatgattgta attttcagct atagcaacag cgtcttcagg tgctataagt gttaatgaat
     4801 ctttgttagc atggatagaa ttgcttactc ctgagagatg caatattagt gcaagaaacg
     4861 taaagaccat tattttccat gaattatttt tagtagaaaa aatataatag atcactatac
     4921 tcatcgtata ggctataaat aaagaagaaa aaccatttaa ccgccagaca aattgtagac
     4981 tcttaactgg tgtatggtta aaaagttgcc aaggaaataa attggttgaa caaaaaagaa
     5041 caaatacacc aagaaagaaa ataaataaat catcagcagt taattttttt ataaaaataa
     5101 aagcaccaat caatccgaga aataagaata atccaatagt atagttgtta tagttattat
     5161 ttaaaatatg tgtcagcata tcactaggag ccataccact taattctttt cccagtggga
     5221 ctttcaattc ttgtgctcta gattgttgaa taaaaggaat taagaatccc gcacttaaaa
     5281 aaaaggtgag gacggtagct tttaaaagac ttaagagtct agcgattttt tggtcccaaa
     5341 aataaaaaga aagaaacaac gttcctccaa taaatacaga aaccatagcg acagaaagta
     5401 aatgagtata cactactagc gtcatgccaa tagacaacca gtaccatttt tggtaatctc
     5461 taatataaat ttcgtaacac cccattagta tcaaaggcaa aaatgttaaa gcgacagctt
     5521 ctcccaatga agcacgatgg aatatgtcaa tggcacgata ggcggaaaat gtataaataa
     5581 tcgaaaataa cagcgaaata taacgattgt tttttatttg tttcatagaa aaataagaaa
     5641 cgaccattgt taaaaaagtg ataaaaaaat agtagatatt ataacttaaa acaagatttc
     5701 ccatcatttt ataaaataaa aaagctggat aaagagtaag ccatggataa aaaatattca
     5761 tcattgttcc atgatgatcg aagttattga agtttactgg agaactccat acattatcta
     5821 gtcctatgat tcgagataaa tgaaagtaag tatcctgatt gacactatga tagattccct
     5881 tagacatgaa tagagggatg acgtaagcaa aacttagaaa taacataaaa atgatatcta
     5941 tta
//
LOCUS       DAZKOK010000141         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000141 DAZKOK010000000
VERSION     DAZKOK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.144"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG90W_002585"
     CDS             complement(488..1297)
                     /locus_tag="ACG90W_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0478887.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG90W_002586"
     CDS             complement(1297..2043)
                     /locus_tag="ACG90W_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0478888.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG90W_002587"
     CDS             complement(2061..2528)
                     /locus_tag="ACG90W_002587"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0478889.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG90W_002588"
     CDS             complement(2528..2938)
                     /locus_tag="ACG90W_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0478890.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG90W_002589"
     CDS             complement(2949..3962)
                     /locus_tag="ACG90W_002589"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0478891.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG90W_002590"
     CDS             4179..4913
                     /locus_tag="ACG90W_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478892.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG90W_002591"
     CDS             4897..5607
                     /locus_tag="ACG90W_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478893.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKOK010000142         5311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000142 DAZKOK010000000
VERSION     DAZKOK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.145"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG90W_002592"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG90W_002592"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0478894.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG90W_002593"
     CDS             complement(1297..2133)
                     /locus_tag="ACG90W_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478895.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG90W_002594"
     CDS             complement(2317..3534)
                     /locus_tag="ACG90W_002594"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0478896.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG90W_002595"
     CDS             complement(3683..3844)
                     /locus_tag="ACG90W_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478897.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG90W_002596"
     tRNA            3917..4000
                     /locus_tag="ACG90W_002596"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5311)
                     /locus_tag="ACG90W_002597"
                     /pseudo
     CDS             complement(4240..5311)
                     /locus_tag="ACG90W_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAZKOK010000143         5190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000143 DAZKOK010000000
VERSION     DAZKOK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.146"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG90W_002598"
     CDS             137..748
                     /locus_tag="ACG90W_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0478898.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG90W_002599"
     CDS             821..1750
                     /locus_tag="ACG90W_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0478899.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG90W_002600"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG90W_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG90W_002601"
     CDS             complement(2693..3895)
                     /locus_tag="ACG90W_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0478900.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG90W_002602"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG90W_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5190)
                     /locus_tag="ACG90W_002603"
     CDS             complement(4706..>5190)
                     /locus_tag="ACG90W_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0478901.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1640 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta
//
LOCUS       DAZKOK010000144         5137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000144 DAZKOK010000000
VERSION     DAZKOK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.147"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACG90W_002604"
     CDS             complement(<1..447)
                     /locus_tag="ACG90W_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478902.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="ACG90W_002605"
     CDS             complement(754..1851)
                     /locus_tag="ACG90W_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0478903.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="ACG90W_002606"
     CDS             complement(1959..3986)
                     /locus_tag="ACG90W_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0478904.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4859)
                     /locus_tag="ACG90W_002607"
     CDS             complement(4131..4859)
                     /locus_tag="ACG90W_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0478905.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1828 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatattt
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgacgagag tgtaaaa
//
LOCUS       DAZKOK010000145         5110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000145 DAZKOK010000000
VERSION     DAZKOK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.148"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..1570
                     /locus_tag="ACG90W_002608"
     CDS             53..1570
                     /locus_tag="ACG90W_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0478906.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1542..2831
                     /locus_tag="ACG90W_002609"
     CDS             1542..2831
                     /locus_tag="ACG90W_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0478907.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3006..3902
                     /locus_tag="ACG90W_002610"
     CDS             3006..3902
                     /locus_tag="ACG90W_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478908.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3916..4779
                     /locus_tag="ACG90W_002611"
     CDS             3916..4779
                     /locus_tag="ACG90W_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0478909.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4785..>5110
                     /locus_tag="ACG90W_002612"
     CDS             4785..>5110
                     /locus_tag="ACG90W_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0478910.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYS"
BASE COUNT         1600 a          800 c         1065 g         1645 t
ORIGIN      
        1 caacgctttc aacgaggtgt attattcatg tataaattaa aggaggtcag ttatggaagc
       61 cacaatgttg attagtagtg tcaagcgtgt aggcaaagtt gatccgcggt tgtacagttc
      121 atttattgaa catatgggac gagcagtata tgaagggatt tatcagccag atcatccatc
      181 agctaataaa agtggatttc gccaagacgt cattcacttg gtaaaagaat tgaacattcc
      241 catgattaga tatcctggtg gaaattttgt gtcaggattt cgttgggaag acagtattgg
      301 gcccatagca aatagaccta gaaggttgga tttagcttgg cggacaattg aaacgaatga
      361 agttgggatt catgaatttt ataactggtg tcagcatgtg ggggccgaaa tcaatatggc
      421 tgtcaatctg ggtactcgtg gagtagatgc agctcgtaat ttagtcgaat attgtaattt
      481 tcctaaagga acttattgga gtgatttaag gcgttcgaat ggtcaagaga gcccttttgg
      541 aattaagaca tggtgtttag ggaatgaaat ggacggtcct tggcagattg gacataaaac
      601 agcggaagaa tatggacggt tagcagagga aacggctaag gcgatgaaac tcgtagatga
      661 ttcaattgag gtggttttat gcggaagttc gaatcggcaa atgcctacat ttggtgattg
      721 ggagttaaca gttcttgatc aagcgtacga tcaaatagat tatttgtctc tgcatcaata
      781 ttatggcaac caaaaaaatg atctttctca ttatcttgca cgttccttag acatggatcg
      841 gttcatttct gaagtgacag cgatgtgcga tacagtcaag gcgaagaaac atagtaagaa
      901 gcagatcaac ctgtcctttg atgaatggaa tatttggtat cattcagcgg aacaagatga
      961 gcaagcagcg ccttggcaga ttgctcctcc tttactagaa gataactata attttgaaga
     1021 tgcattacta ttaggctgtc tattaatcac tttattgaaa catgcagata gagtaaagat
     1081 tgcttgtctt gctcaattgg taaacgttat tgcaccaatc aggacagaaa caaacggaga
     1141 agcttggaga cagacgatct attatccatt tatgcaagct gctgtgtatg gacaaggaga
     1201 ggtgttaagc ccacaaattc gttcagcaaa ctatgcgacg gaagattttg aagaagtgcc
     1261 gtatttagaa agtatagccg taataaatga caaagaactt gtcatatttg cagttaaccg
     1321 tggagaagaa gaaatgaatc taactgttca cttgtctgaa ttatcattag agggagtgat
     1381 tgatttttct gaaatgagtg gatttgatac aaaagcaact aatcttccta aaagtgaaca
     1441 agtaaaacca agtcattcaa caaatgtaaa agctgaagat gggaaaatca attgtttatt
     1501 gaaaccgcta tcttggaatg tggtcagatg tcgtttatca gatgaaggag gaacaaaaaa
     1561 tgaaaggtaa aaaatggtta attggctctt tgttggtagc tgcaacaggt attttaggag
     1621 cttgcggtgg cggaagttca gcagaatctg attctgataa agaagtgact ttgacttttt
     1681 ggaatgggtt tactgcatct gatggtgaaa tattgcaaga aatcgttaat gaatttaata
     1741 aaacaaatga taaaaacatc aaaattgaaa tggatatcat gacttgggcc aatttgaatg
     1801 aaaaacttcc tacagcgatt tcttctaaaa aagcacctga ctttattgca ttaaattatc
     1861 cagattttgc acagtatgta gaaaacggag cggtacaacc attagacgat ttctgggaat
     1921 atgatggagt agataaatct gattttaaag aaacggctat tgaattaggt gaagtagaag
     1981 gtaaacagta ttttgtcccc atgcaggtac aaggcatgta tatgtattgg aataatgatt
     2041 tgtttaaaca agctggatta gatactgaac aaccacctac tacttgggaa gaattgacag
     2101 aaatggcacc aaaattaact gatagtagta aaaatgtatc aggttttgtc ttcaataagg
     2161 atggaactgc accgttatat aactggatgt tagcaaatgg cggtaaatta gtgaacgatg
     2221 attatactaa gtctgaattt gcgtctcctg agaaccttaa aacgttaaaa gcgatccaaa
     2281 aaatgatcca tgtagacaag actggtccag aatcgatttc tggcgccgaa atggacaatt
     2341 taatgaatgc tggtcaacta gccattgaaa tcaatgggcc atggttaaat aacggattga
     2401 aagcaaacga gattgattat aatgtgacta ccttaccaca agggagtgat tcagacaaaa
     2461 aaacagctat tttagatggc gtaggatatg ccattccggc aagtacagat aataataaaa
     2521 aagaagctgt ctatgagttt ttgaaatatt ggaatacaac tgaaattggt aaaaaatgga
     2581 gtatcgaaaa tggttttcct gcctacttgg attcagtagc aaaagatgaa gaagtaaaaa
     2641 atgatcctat tgtatctgaa ttatcgaaac aaatggacta tgcagagcca tatttaccag
     2701 gttttaccca aatctctgcg atcaataacg atatcatcaa tccactgatt gaaaaattat
     2761 tagcaggaga agatcctgag acactcatga acgatgcaga tgatgccatc aatcaattac
     2821 tgcaaaaata gtacagaact ttatacagga tagactgttg gcaagataca gatatctgaa
     2881 tgatcgacct agtatttgca gataggtgtt attgcaaaaa tcacagcgat tgttcggata
     2941 tcttattcag attcgtttaa accaagattg tatattttat caaaatgaaa gatgaggtgg
     3001 actatatgaa cggattcacc aaatattgga atcgtcctga tcgtgctgcg tatttgtttt
     3061 taactccatc ttttttagtt ttattattat ttactgtcat accgctttta ggaacatttg
     3121 ctattagttt tacagatttg aatattttct ttacatcaag aaattttgta agttttgaaa
     3181 atttcacacg tatatttagt gatgaacgag cagttaattc gttgattcat tctttatatt
     3241 tcaccgtact cgaaacgcca acacaaatta tcgttggttt attagcagca gtagcattat
     3301 ctaaaaatac cagattcaat cgtttttgtc gttcgacatt ttacattcca gttatttgtt
     3361 cattgacgtc aattgcgatt attttttcta tgttgcttga tccgaatgta ggggcgattc
     3421 cttacttgtt ttcacaagtc ggcttgccga tccctcaatt ttttcgcgat ccaacccttg
     3481 ctatgccgac ggtagccatc atgactgttt ggaagaattt tggtgttact atgacgattc
     3541 tcttgactgc tattcaggga atctctccct ctttgtatga atctgctcaa atggatggtg
     3601 caacaaatcg acagcagttt tttaacatta cgctacctca gatcgttccc tccttaggtt
     3661 tttgtatttt aacgaatctg attggttcga tgcaagtgtt tgaccaagta tatgttgcaa
     3721 ccgctggtgg cccacaattt aagacagaaa cagctgttca atatatttac agtcgaggat
     3781 ttacagcacc ttatgaattg ggctatgcat cagcactgtc ttcggttttg tttatcattg
     3841 ttgcaactat agctatctcc actaatgtgt acatgtcacg gaaagaaaaa caaatgaaat
     3901 aggagggaaa cctgtatggc accagaaagc tcgattacag ttactgtgaa tagtcgaaaa
     3961 gcagttagtg gtaaaaaaat cattcgaacg attagtatgc tagttatatt gttggttatt
     4021 gcgtttcctt ttttgtggct gattatttct tcttttaaac acgaaaaaga cattatctca
     4081 tttccgccta gaatatttgc agatagctat accctagata actatattaa ggtttggaca
     4141 acgatccctt tattagatta catcaaaaat acagtgattt ttgctggagg gacagtgatc
     4201 acttctgtat tctttgattc tcttgcgggg tatgcatttg ctcgtatgcg tttcaaagga
     4261 aaatcagttt tgttctattt cgttttgctg acaatgatga tcccattcca agtcttcatg
     4321 attcctctat ttattgaagt caatttgctt ggcatgcttg atacttatgc aggtttgatc
     4381 attcctcgta tgacaactgc atttggtatc ttcatgatgc gttccttttt tattacttta
     4441 ccagattctt tggaagaggc agcacgcatt gatggattaa gtgagtttaa tatttttttg
     4501 aaaatcatgt tgccattgag caaacctact cttttatcat tagggatttt cacgctgatg
     4561 aatagttgga atgacttgct ttatccattg atattgacaa gtagttcaaa aatgagaact
     4621 ttaccagcag ggttagcttt atttacaggg cagaacattt ctttttatgg acctgtaatg
     4681 gctggaacgg ttatttcaat gctgccatta ctagtggttt atatttttgc acagaaatat
     4741 tttgttcaag gaacagctat gtctggaatg aaagagtgag gaagatgaaa atagttgaga
     4801 ctaaatttta tggacagttg attgtggttt ctgattacat cttattaggt attctctggg
     4861 taatcgtgtg tttgcctgtt attactatcg tgcctgctac atgtgctgtt ttttatgtaa
     4921 tggcgcaatg gaaaggtgag ggatcaggaa atatcattta ttttttcttt caaggaataa
     4981 agcaacattt tggtaagaac ttgttcctta gtctgttaac gatcattatt tattttcttg
     5041 ttaatcaatt attagaagaa cagacaatcg tactttcaat tagtggatat gtagttttac
     5101 ttatttattc
//
LOCUS       DAZKOK010000146         5073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000146 DAZKOK010000000
VERSION     DAZKOK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.149"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1818
                     /locus_tag="ACG90W_002613"
     CDS             103..1818
                     /locus_tag="ACG90W_002613"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478911.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1821..3584
                     /locus_tag="ACG90W_002614"
     CDS             1821..3584
                     /locus_tag="ACG90W_002614"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478912.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3624..4160)
                     /locus_tag="ACG90W_002615"
     CDS             complement(3624..4160)
                     /locus_tag="ACG90W_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0478913.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     regulatory      complement(4198..4296)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            complement(4483..4905)
                     /gene="mgsA"
                     /locus_tag="ACG90W_002616"
     CDS             complement(4483..4905)
                     /gene="mgsA"
                     /locus_tag="ACG90W_002616"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0478914.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1520 a         1019 c          983 g         1551 t
ORIGIN      
        1 ttatgaaaag gcttgttctc ttttcattct aaatttccta agctacaatg tggtagtact
       61 tgttttttca attgtggaca aataaataat aggaggaatc atatgattca gttattacgt
      121 tatgcaaaag actatcgtaa gcaaatcatt ttgggcccgt tcttcaaatt tcttgaagct
      181 gtcttcgaac ttgtcctgcc tttaatgatg gcttccttga tcgataatgg actaaaaatg
      241 aatgaccgag gaaaaatcat tgaaatgggc ttatggatgg tcgctatgtc tgtgattggt
      301 ctgatttgtg ctatcatttg ccaatactat gcttcagtag cttcacaagg atttggtaca
      361 gagttacgta atcagctaat caaaaaaatc aatactttct ctcacaacga gttgaatcat
      421 tttggaacag ataccttgat cacccgaatg acaaacgaca tcaatcagct tcagctagct
      481 ttagcaatgg tcattcgttt attgatcaga gcaccgtttt taagtatcgg gtcagtggtc
      541 atggcttttg tgatcgactg ggaagtaggt ctgtttttct tagcattatt gcctattttc
      601 tcaatcattt tattttttat catcaaaaaa accgttcctc tctaccaaaa agtgcaagaa
      661 aaactagatc agctcaatga acacgttagt cagaatctta gcggtgttcg tgtcatccgc
      721 gcatttgcgc agacaaaaaa agaaacagag aagttcgata aaagcacaaa tgatctagcg
      781 aacaattatt tacgtgtttc caatatctct gccctattat cacctgctac aaccttgatc
      841 atgaatgtcg ggatcatctg tttgctgaca gttggcggga tcaaagtaaa tattggtagc
      901 ttgcaacaag gacaagttct tgctttgatc aattatatga atcaaatgct tttggcactg
      961 atcgttgttt caaacctcgt cgtgatcttc acacgtgccg aagcttcagg aaatcgggtg
     1021 aaagaggtgc tagacacaga aaattcgatt cttgaagctg aggtcagtac tactcccgat
     1081 ttttcttctg aagcaattat cgcttttgac catgttgact tccgttatac tcctgattct
     1141 ggtctttctc tccaaaatat tacatttcag ctgaagcgaa atacagtcct tggaatcatt
     1201 ggtccaaccg gaagcgggaa aacgactttg acacaattga ttccgcgttt ctatgatgtc
     1261 agttccggta cagttataat ggacggaatg gatgttcgtt cttggcagct ggatcatctc
     1321 cgtcagcaaa ttgctcttgt tccccaaact tctgtactat ttaccgggac tattcgtgaa
     1381 aaccttcaat ggggtaagaa agacgccaca gaaaaagaat gttgggaagc tctgaaaatc
     1441 gctcaggcag aagaatttgt ccgtcagctt ccaaatggtt tagatacaaa agtcatggaa
     1501 aatgggaaaa atttctctgg cggacaaaaa cagcgactga cgattgcaag agctttgatt
     1561 gccaagccag cattactgat tttagatgac tctttaagtg cgttggatta tcaaacagac
     1621 ttggatttgc gtaaggcgct acagacttat cttggcagta cagtgattat tatctcccaa
     1681 cgtgtccgct ctattcaaga agcacatcat atcttagtga tggatcaagg taagattgct
     1741 gctcagggta cccatgaaga attacttgct caatcagcag aatatcagga aatcgtcgca
     1801 tcacaggagg aagaatagtt atgaaagtaa aaaaattatc cttgcagacg ctccgccgat
     1861 tcattcctta ccttactgcc tatgcaaaag acatttggct agctgtgata ctaggaatca
     1921 tcaatgggat tgcaacagtt gtgatgacgt tacaaattgg tcaaagtatc gatcaaatga
     1981 ttggagaagg acaggtccgc ttcaatcgac tgttccattt acttattcta ttcggtggta
     2041 tcgttttaat caacgtagtc agtcaatggc tcatccaagt gttaagcaat cggttagctt
     2101 acttatccgt tgcaaaatta agaaaagatg catttgctca tctgaatcga ttgccacttc
     2161 gttattatga tcaaaactcc cacggaaata tcgtcagtcg atttacaaat gatatggaca
     2221 atatttccat tgcaatttct gcggccttca atcaattatt ttctggagtg gcaatcatca
     2281 ttctttcgtt tatttttatg atgcgtctaa gtccacttct gacattggtc gttctttgtt
     2341 ctacaccaat tattttcctt gtgagttgga tcgttgctcg tgcttcacaa acaaatttcg
     2401 atagacagca aaggattatt ggggatatct cgggctttgt gaccgaacga atcggtaacc
     2461 agaaaatcgt gaaagctttt cagcaagaag aagctagtca acagcaattt gaactgctaa
     2521 atgctgatct atatgaaaaa ggacaacggg cacaattttc ttcttccttg acaaacccgt
     2581 cttctcgttt tgtagatcac cttgcttatt tatcgattgg tttagttggc ggcctattgg
     2641 cattgaagag tggttcgtca atcactgttg gtgtgatttc cagctttacg atctactcaa
     2701 gtcagttttc taaaccattt atcgaacttt ctggaatcac gacacaactg caaaccgctt
     2761 tttctggtct agaacgagca tttgatttga tcgatcagcc agaagaaaag cctgatttgc
     2821 cagatgccta tgccttggaa cccaaaaata tcaaaggaaa agtctctttt gatcatgttt
     2881 ctttttctta tgaccccaag cagcgtttga tcgaagattt caattttact gcttatccag
     2941 gtcaaaccat tgctatagta ggaaaaacag gggccggaaa atccacactt gttaatttgc
     3001 tgatgcgttt ttatgacaca acaaaaggaa caattacagt cgatgaccat gacatccgct
     3061 ctatccaacg tgatacatta cgtaaaagtt ttggaatggt gttacaagat acttggctat
     3121 ttgacagtac attgcgagaa aatcttcgtt ttgggaatcc ggaagcaagt gacgcagaaa
     3181 tcaatgatgc cctaaaagcc tcttatatgt atgaatttgt agaacgtttg ccggaaaaat
     3241 tagatactaa gattggcggt caagggttaa agatatcaga tggacaacgc caactgttga
     3301 cgattgctcg aacgatgatc agtaatcccc ctatgttgat cttagatgaa gctaccagct
     3361 cagtcgatac attgacagag aaaaaaatcc aacaagcctt cttagcaatg atggaaggaa
     3421 aaaccagttt tgtgattgcc catcgcctgt ccacaatcaa aaatgcggac cagatattag
     3481 tgatggatca gggagctatt gtcgaaatgg gcacccatga tgagctgctt caaaaagatg
     3541 gttattaccg acggctctat caagcacaat tcacagcaca ataaaaacaa gcagtcttct
     3601 gggatgggaa gactgcttgt tttttattta gcgtggatcg ctaaacttct taaaattttt
     3661 ttgtaaggaa tactacgtcc tacgtaataa atggcaattg attgaatcgg taaccatatt
     3721 actgttttta acaggcgggg cgcccaaacg acccagctga acaatggcac atggtacatc
     3781 aacgcaagcc ataatggtgt caaaaataag ttgatgatca gcgtgacact aagcgtagcc
     3841 agaatggcat ttttccaagt gatctctttt cgatagaaaa agaatccgta gattgctcct
     3901 tcaataaatg cattcaatgt aaatccaata aagaatgcag atttcgagaa gagggccatt
     3961 cctactaaat cagctaaaac tgcgcctatc cctgaccaaa aaggaccaaa aagaatgccc
     4021 atgatcactt gtggaataaa tgtgacactg attttaataa atggggtctc gattgctacc
     4081 agtcgggaca aagtgaccat caatgcaatc aataagccca taatagcaat catacgtgcg
     4141 tctaaccggt gtttcttcat ttttagcatc tcctttaaga gctgctacat cgtatcatgc
     4201 agcgaatgcg aaaataaaac cgcgaacatt acgtccttcg tccaagaggc aacatcccat
     4261 cctcttagca cttaacgctt tattttctac tctgtattat ttttgggatc ttttcccaca
     4321 gaaactatag catattttca catttactgc aaatacgaaa aactaatatt attgtttata
     4381 ttatccaata cgttcgtatt atatcgcata ttatcagttt gtttagcttt tatagctaca
     4441 tataggcgaa cattaaacgt tatttttcca tacgcttatc catcaaaatg aaagtggatg
     4501 cttgtctatt tcatggacga ttcttcgaaa atctaggaag ccggcttcca gtcctcttag
     4561 tagcacctca gctgttccga tattcgtagc taacggaatc tcatagacat cagataaacg
     4621 gataagtgct gttacatctg gttcatgggg ctgtgcagcc agcgggtctc gaagaaaaat
     4681 caccaaatct aactgatcct ccgaaatcat ggctccaatt tgctgatccc cgcctaacgg
     4741 tccggattta aaccgatgga tcggcaagcc ggttgcttcc gtgatcctct gcccagtcgt
     4801 cccggtagca aaaagttcat gtttttccaa tattgtttta tacgctgttg ctaatttcac
     4861 catcataggt ttttttcggt catgagcgat caaagcgatt ttcataagag acactccttt
     4921 tgatattgtt aacatactta tttactccag tgtatcagtt tttatttctt atggatattt
     4981 attaaagctt aaaactagca cattgacaaa aaaaagaaaa aattcttgtt caacatgaca
     5041 ttgaagttag ttaacgcttt catttatact tta
//
LOCUS       DAZKOK010000147         4964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000147 DAZKOK010000000
VERSION     DAZKOK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.150"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..836)
                     /locus_tag="ACG90W_002617"
     CDS             complement(63..836)
                     /locus_tag="ACG90W_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0478915.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            980..1330
                     /locus_tag="ACG90W_002618"
     CDS             980..1330
                     /locus_tag="ACG90W_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0478916.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1311..2027
                     /locus_tag="ACG90W_002619"
     CDS             1311..2027
                     /locus_tag="ACG90W_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0478917.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2027..3475
                     /locus_tag="ACG90W_002620"
     CDS             2027..3475
                     /locus_tag="ACG90W_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0478918.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEEKNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(3545..4054)
                     /locus_tag="ACG90W_002621"
     CDS             complement(3545..4054)
                     /locus_tag="ACG90W_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478919.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4044..4769)
                     /locus_tag="ACG90W_002622"
     CDS             complement(4044..4769)
                     /locus_tag="ACG90W_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478920.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1705 a          847 c          996 g         1416 t
ORIGIN      
        1 cccctttctt aagaaaaaat aagcgatttt cttaagaaaa gaggtagctt caaaaaaaga
       61 tcttaaatca gatcaaaata cttcaatgcg tggacaattc cgccttctgt atttttcttt
      121 gtgataaagg tagaaagttc ttttaatccg tcataggcgt tgcccatagc gattttgttg
      181 tcgcaagctt ccaacaaggc aaaatcattc ggaccatcgc caaatccaaa agtaggaact
      241 ttttccagct gaagttcttt ttgcagacgt ttgacacctg ccccttttga gattcctttc
      301 ttcactgtat cgatcgagta aggaccattc cgataaaaag ttagctcagg aaaatgttcg
      361 tgatagtatt cgtcacctga ttgagataag atcagcaaca tatttactgt gttttcttta
      421 aagcctaaag gatcaatttc cggaacatct gaatgaatga agtcgtaagc attgatcatt
      481 gtttggctat gaccggtaca ccagattttc tgatcgttat agtaagaaag ttcatgaccg
      541 ttcaactgta catgatcata cattttttga cattcttctg aagaaaatgt ttgagaatag
      601 atttcttcgc cgtctactcg aatgaaagaa ccattcatgg tgaccgcaga agtaatgccc
      661 gcatcttcca tgatagagtg gatttctgct tctgtacgtc ctgtagcgat gacggggaga
      721 acattgtttt cttttaatgc gttcatagct tttgccactt cgggtgtgat ttttgatttc
      781 gcatctagta atgttccatc aagatcaaag aaagtgattg cacgataatt tgacacaaat
      841 aaaactcctt ttttattgga ataagtttat cttaacaaag aatgaacaaa agacaaacct
      901 ttcgtgaaga attcctgaac atattggttc agtcaacaat ttagagtata ctataagaag
      961 agaatttagg agggaaagaa tgattttttt cagtattggt atcttattct taataattgg
     1021 cagtcttttt cggattctgc cctcaaaagg aaatctgcct ttttatgggt atcattcgcc
     1081 tcttgccgca aaaacagacg ctcattggcg gctggctcaa aagacaagcg gaaactggtt
     1141 ttttcttatg ggcttactga tggcattgat cggctattat ttaaagacat ctgggcatac
     1201 gaattatttt ctgattgaaa tgcttctgct agtatttcca attatgccta tttttattat
     1261 gacagaaaag aaattacaga aatatgacct ggaaactgga gggaacgata atgaatattt
     1321 taatgattga agataatcaa tctgtttctg aaatgatgca gatgtttttc ttaaatgaag
     1381 gttgggaagc aactttcaag tatgatggaa aagaagggct cgatgccttt ttagaaaacc
     1441 cacaacattg ggatatgatt acactagacc tgaatttgcc cacactggac ggtatgctag
     1501 taggaagaga gatccgcaaa gtttcaagta ctgtgccgat tatcatgctg acagctagag
     1561 attctgaaag cgatcaggtg atcggcttag agatgggggc agatgattat gtcacaaaac
     1621 ctttcagtcc tctgacgctt attgctcgaa tgaaagcttt gcatcgccgt agtgagttgg
     1681 cagaagcaag agaagaagca gtagtttcag atgatcattt tgatatcgag actagtcatt
     1741 tcaagatgaa tacgaaaaca cgagaagcgt atctggacaa ccggccaatt gaaggactga
     1801 cgcctaaaga gttcgatcta ttgtttactt tagcaaaaaa accacgtcaa gtattctctc
     1861 gggagcagtt gcttgaatta gtctgggatt atcagtactt tggtgatgaa cggacagtgg
     1921 acgcccatat caagaaatta cgacaaaaaa tcgaaaaggt cggaccacaa gtcatccaaa
     1981 ctgtttgggg agtcggctac aaattcgatg attctggtgt tgcctaatgc gttatttgta
     2041 tcagcaactc ttagctttct gcggaatggt cgcgttgatc ttattgatcg ttggagtttc
     2101 ttttacacaa ctgacaaaac aaacgattga agaaaaaaat taccagcaac tgtttggata
     2161 tgctgaatca gttgaaaaga caacgcaaac ctatgccgat acgctcccgc agtttagtca
     2221 agatcaggcg tttcaaaatg cattgctgct gacagagcaa gtactgacag aacaaaatgt
     2281 gaactttata tttatcgata aaaacgaacg tgtcatttat ccgactaata cagctttact
     2341 gaattttacc ttaacagctg atcaatggaa aaatctgagg aatggacgtc aagaaaaatt
     2401 cacatcgaat aaaaatattt taggacaagc acaggcgacc tcttacgcac ttgtgccatt
     2461 taatttgaat catgaattct atggagcact tgtagttagt caacctgcgc gaaatatcga
     2521 taacagcgtt cgtccaatta cactgaacct gttcaaagga tttatttttt ccagtatcgt
     2581 tgcagttatt gccagctatg cttttgcagc attccaagtt aagcgtatca atcggttaag
     2641 gaatgcgacg aaagaagtga cgaatggtaa ttttgatgta cagctccccg tacatgacaa
     2701 agatgaattt gatgagttag cagatgattt caataaaatg acgaattcat tgaaagaatc
     2761 tcaagcagaa attgaagaac aagaaaatcg tcgaagacaa tttatggcag atgcttctca
     2821 cgagatgaga acacctctta cgacaatcaa cggacttctg gaagggttag aatacaatgc
     2881 aatccctgaa aatcagcgag aaaacgcgat taaattgatg aaaaatgaaa cagagcgtct
     2941 gatccgtttg gtgaatgaaa acttggatta tgaaaaaatc cgtacgaatc aaatcagtat
     3001 ggtcattaaa aaattcaatg gaacagaaac attgaaaaat attgttgcac agttagaagc
     3061 taaagcagaa gcagcaggag atacattgac attgaaagcc gttgatgata ttgatgttta
     3121 cgctgattat gaccgatttg tccaaatcat ggtcaatatt attcaaaacg cgatacagtt
     3181 tactgaaaac ggccaaatca cgattacttt agaaaaaggc tatcttgaaa cgatcatcac
     3241 gattgaagat acgggaattg gaatgtctga acaacaaatg aaaagtatct gggatcgtta
     3301 ttataaagta gatccttcaa gaaaaaatac aaaatatggt gaatcaggat taggtttacc
     3361 aatcgtgcag caacttgtac gcttgcataa aggaaaatta gaagtagaaa gcgaactagg
     3421 aaaaggaaca aaattcgctg ttcgtttgcc tgacgtagaa atcaaaaaag agtaaaaaac
     3481 aagcggtata aaagatcatg aatcgatctt ctataccgct tgtttttact ataaaatgat
     3541 tgatttagcc cgcgggctct aaacgatcaa tataaggact ttgagcaata gtagcatcat
     3601 gagtaaagag taagatacct tgattttctt catgagctaa ataagaaagc aagtggagta
     3661 gctcttggat ctccaataca ctaagttcat caaatgtatc ttcaataaca atattgtttc
     3721 gcttggaaag caaggcatga agtaattgca gctttacttt ttcaaaagac gtcaactcag
     3781 ctgccgcaca attaagcaag gaaggagaga tatcaagttt ttcttgatag gccaaacgat
     3841 cttgtttgat ttctttttcc ggtacatcca acaggacatg gtctttaaga ttcagataag
     3901 gaaacatcgg ccaatttttt tggatgatcg caaaattatc cagttttgca acaagaacct
     3961 cttttttcaa gtgctccaat attttatgct gtttttcgag aggacacaaa ataacggtca
     4021 agtgttcagc atgaatcaaa tattcaggtt tcattacgtc caggcctcct actttgcaaa
     4081 tgatggataa aaaaagtgaa agggaataga ctaagaatga gacagcatcc actgagccca
     4141 ataagagttt gggcaaatcc cttaagtata tctgtgaaaa aaagatcatt ttggaaatcg
     4201 atggtaaaaa aagtctgttg atccatcgga attgtaagtc cgcttaccga agtcaagata
     4261 tcacggatat ctggcagttc agtagtcttt aagacaagaa aattgatcct ttgaagaagc
     4321 gcttcataaa ctgactggaa gcttacgatc cacaataaaa ccaagataca acaaagaaag
     4381 agaggaaaga acagctgcca gaaaataaaa aggaataatt tggttttaga aagaccagtc
     4441 aggtgccaat tgatcaattc ctgcttgttt tgcctgactg aatagtaaaa gaaaaagata
     4501 aaaaggaagg caaatagaaa aaataaactg aatatgcctt ttatataaaa tcggctagct
     4561 aattctaaag ccggaagaag ctctgtagat gcagaagcgc caataaatga aagccgatct
     4621 tggacttgat tggctaaagc cgattgcaaa tggtaaagag tcaggcatgc tatcaataaa
     4681 aaagcagaaa agcttgaaaa taaacaatag agtattgtct gctttttgta ataaattaat
     4741 cgagcagtag catagcggat tattttcata cttttcacct ctataaaaag tatagaaaat
     4801 gaatatgaat aaagtatgaa attcctttag tgaaatcacg aattaaaacc agaaaatatt
     4861 ttgtaaaaag caagaggttg tgaagaagct gccctattct attaccgtag atgcaagctt
     4921 ttgaacactg tttattcgaa aagaggagca gcgacaataa tttt
//
LOCUS       DAZKOK010000148         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000148 DAZKOK010000000
VERSION     DAZKOK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.151"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG90W_002623"
     CDS             85..972
                     /locus_tag="ACG90W_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0478921.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG90W_002624"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG90W_002624"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0478922.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG90W_002625"
     CDS             2274..2720
                     /locus_tag="ACG90W_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0478923.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG90W_002626"
     CDS             2993..4345
                     /locus_tag="ACG90W_002626"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0478924.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG90W_002627"
     CDS             4516..4803
                     /locus_tag="ACG90W_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0478925.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKOK010000149         4746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000149 DAZKOK010000000
VERSION     DAZKOK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.152"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..1100)
                     /locus_tag="ACG90W_002628"
     CDS             complement(138..1100)
                     /locus_tag="ACG90W_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0478926.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1102..2541)
                     /locus_tag="ACG90W_002629"
     CDS             complement(1102..2541)
                     /locus_tag="ACG90W_002629"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0478927.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2726..4672
                     /locus_tag="ACG90W_002630"
     CDS             2726..4672
                     /locus_tag="ACG90W_002630"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0478928.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1509 a          812 c          780 g         1645 t
ORIGIN      
        1 gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacaagttga caactccttt
       61 ttgtttacaa atcaattgga tatctacaaa ttgtattttc taacgaattt tatagacatt
      121 aaaaaattaa tttaacgtta aatacttcct ccagaaagta ttgagattgg taaaatataa
      181 tccttatttg tcttttcgcc tttaattttt ttcagaagaa tatcaacaat aagacttgca
      241 atttcatcaa ttggttgtct tattgttgtc aattgtggga aaaatttctc aataaaagag
      301 gtaccatcgt atccaataat ttttaaatct tctgggatat taagtttaag ttgatgagca
      361 attttcatag tgagaatggc tgttaaatca tcagaaacaa aaataccatc tggtttgtga
      421 tttataatag ttgactttat ttccatttca cgacggatag tagacagatt attaggaatt
      481 ttgaaaattt ttccatttgg attttgaaaa gaaaaaccta gtgcacgtaa ttctgtggga
      541 gaatccgtgt tatcatttcc tgtaatcata atggtatttt gacaaccatt tttttgtaaa
      601 gtcttagctg caagttttcc cccttcaaag ttatctgaag aaacaatgga aacattagga
      661 gctaaatttc tatcaaaagc aacaataggt gctctcacac gctcataatc agcaattcca
      721 agattatggc ttgatgagat tatgccatca acttggttgg cttcaagcat ttcaatgtat
      781 tctctctctt tagcaggatc attttgacta ttgcaaataa tcgttttata tccatgttta
      841 aataactcta tttcgagata ctcaatgagt tcagagtaaa agatattact aatattagga
      901 aaaataagac caataagctg aggtgacttt ccttgaagtc cacgtgctaa attatttggt
      961 ttataaccaa gagttttcat agcttcatga actttttgtt ttgttttttc tgataaatat
     1021 cccttattat taatgacgcg tgaaacggtt gttgggctaa ctccagctaa ttcagcgaca
     1081 tcagttaatt ttgctaccat tttaatattt taattcaaag tattttccag taggttcgcc
     1141 tgatttgatt tgaataccat tttggtgatt ttcagggaat acacgactag taaacacttt
     1201 ttctccttta ttaataaaaa tctcaatgat tgagttatct acaaaaatgt tagcagttgt
     1261 ttctttagta tcaattgagc attcacgaat cgtaccaaag tcagtggcat attgaatgcc
     1321 tgctttgctg cgatctaaaa taatttttcc ttctttggta tctacaatca agctaagtcc
     1381 atttccttta ctatcagaga aaagaagtaa ctccgatttc tgatttggag gaaaagttag
     1441 ttcaagctca taagtattat ttgtttctgc ttttgctgta aattgttcag aactagcacg
     1501 taatgatgta atagcttcta ctggatattg gtaaagttta ccatctttta ttgaaagttc
     1561 ttttacaaga ctcattgctc cctgataatc atacttatca gttggataat cgatatcagg
     1621 tagtccaatc caactgacaa tcagagcacg accatcagga gtattgaatc cttgagtcgc
     1681 atacgcttca aatccataat caagattttg aatttctgaa gcgcccacaa gttttacttt
     1741 ttctatgtca agcgattgac agatcttaaa agtattcgga tagatattcc cataatttag
     1801 ctcagattta tccaaccctt gaggacaata aatgagaaca ggttttttgt ctacaaatac
     1861 tagatttgga cactcaatca tgtattctga accagtgccg ccaaaatcaa ggttgccaac
     1921 ttcctcccat gtttccacgt tattgtcatc cgctttgtaa agtttgataa agcctgattt
     1981 ttctaaattt tgtgctccaa caatcgcata caattgtcct tcataattaa acatttgtgg
     2041 atcacgaaaa tgatcagtta catcagcagg tttatgaata agaacattct cacctttcgt
     2101 aatgttgtag tctttatcca tccatgctcc gatttgaagt gtatcacgtt cccaattttc
     2161 atttcgaacg tttcctgtat agagtaagaa tagattcccc tctatttcat atgcagaacc
     2221 ggaataagcg ccgtggctat ctcgttttgt gtctggaaga agttctgctc ctgttacatg
     2281 aaaatgaact aaatcatctg attcattgtg tacccattct ttcaacccat gagcagctcc
     2341 aaatggccaa ttttgataaa aaagcgtata ttttccgtta aaaaaagaaa aaccattagg
     2401 atcgtttaac aatcctgttt taggttcgat atgatagcta gcacgccaag gtgatcgttt
     2461 tacattatcc ttaattttat ccatctcatc tttagtccaa tcagaataag cacgatatcg
     2521 tatttctcga ggtaagttca tattttactc cttcaattta tctttttaca actagtatgg
     2581 taaacgtttt ccgttgaaaa atcaatcatt tcatttaaaa aaataaacta tatgacaaac
     2641 gtttgacata tttttaagat atgctaaaat aaatttgcat ttcagtgaac cgttttcacg
     2701 taaaaataaa aaaggagtga ttcgaatgaa ttacagtaaa gtagcttccg acgtaataca
     2761 agcggtcgga aaagataacc ttattgcagc tgcacactgc gctacacgtc ttcgtctcgt
     2821 actaaaagat gacacgaaag tgaatcaaaa agctttagac gaaaatccag atgtgaaagg
     2881 cacatttaaa attgatggac aatatcaagt gattatcggt gcaggtgatg ttaatttcgt
     2941 ttatgacgaa cttatcaaaa aaactggact ttcggaaatt tcaacagatg atttaaaaca
     3001 aattgctagt aaagataaaa aattcaaccc cattatggca ttaattaaat tattatcaga
     3061 tatttttgtt ccaattatac ctgcccttgt tgcaggtggt ttattaatgg cattacaaaa
     3121 ctttttaaca gctgctggac tttttggacc aaaatcaatt gaagaaatgt atcctgcgat
     3181 tgaaggtatt tcctcaatga tccaactgat gtccgctgca ccattcatgt tcttaccaat
     3241 tttagtaggt atttcagctg ctaaacgttt tggagcaaac caatttcttg gtgctgcaat
     3301 tggtatgatc atgacaacac cagatcttgg tggctcaact gaatattgga atatttttgg
     3361 ttatcatgtt tcacaaacaa actatgctta tcaagttatt ccagttttag cagctgtttg
     3421 gatattatct atattagaaa aattctttca taaaaaattg ccatcatcta ttgactttac
     3481 attcacgcca ttattatcag taatgattac tggatttttg actttcacag ttattggccc
     3541 tgtaatgtta ttgttgtcta atggaattac agacattatt gtttggctat ataacactac
     3601 tggatttatt ggaatgggta tttttggagg aacatattca cttatagtca tgactggttt
     3661 acatcaatca tttccagcta ttgaaactca attactttca gcgtggacaa atggtatcgg
     3721 acatggagac tttatctttg tagttgcatc tatggcaaac gttgctcaag gtgctgcaac
     3781 atttgccatt tggttcttaa ctaaaaattc taagacaaaa agtcttgctt cctctgcggg
     3841 tgtctcagca cttcttggaa ttacagagcc tgccttgttc ggagttaact taaaataccg
     3901 ctttccattc ttttgtgctt taattggttc aggaattgct acagcagtag caggattact
     3961 taaagtagtt gctgtttcac ttggttcagc aggtttcttg ggatttcttt caattaatgc
     4021 aacatctatt ccattttacg tattgtgtga attgattagc tttgtagttg cttttgcaat
     4081 tacttacttc tacggtaaaa caaaagctgc tggtatcttt aaagcagaag aaattgctga
     4141 acaaacacca gttaattctt caaaagtagc aacaacagat ccaatatcta ctgatgttga
     4201 atcaacacaa acaaaagttt catcagaaac aattgttagt cccatttctg gtaaagtgat
     4261 tagtctttct tcagtaaatg atccagtctt ttcatcagaa gctatgggaa aagggattgc
     4321 cattaaacca aatggaaaca cggtccattc tccagtagat ggtactattc aactcacgtt
     4381 tgaaactgga catgcttatg gtattaaatc aaataatggt gctgaagttc taattcatat
     4441 tggtattgat actgtatcaa tgggaggaaa aggatttaca caaaaagtta ctactaatca
     4501 aaaagttaga aaaggcgata ttcttggagc atttgaccgt tcaatgattg agaaagaagg
     4561 tcttgatgat acaacaatga ttattgtgac caatacttcg gattattcag aagtaaatat
     4621 agtagctgaa aatacagtca ctgagggtac tgatttactt gaaatcaaat aaatatattt
     4681 ccctgagtaa agatggaatt ttctttccaa aatcaaataa tatagattaa tcaaatattt
     4741 gagaat
//
LOCUS       DAZKOK010000150         4678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000150 DAZKOK010000000
VERSION     DAZKOK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.153"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(415..1023)
                     /locus_tag="ACG90W_002631"
     CDS             complement(415..1023)
                     /locus_tag="ACG90W_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0478929.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(1029..2060)
                     /gene="vanA"
                     /locus_tag="ACG90W_002632"
     CDS             complement(1029..2060)
                     /gene="vanA"
                     /locus_tag="ACG90W_002632"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0478930.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(2053..3021)
                     /locus_tag="ACG90W_002633"
     CDS             complement(2053..3021)
                     /locus_tag="ACG90W_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0478931.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3353..4645
                     /locus_tag="ACG90W_002634"
     CDS             3353..4645
                     /locus_tag="ACG90W_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFQ0478932.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT         1338 a         1075 c          881 g         1384 t
ORIGIN      
        1 cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa acggcacaat
       61 ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc gccctactgg
      121 atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc tgaatttaac
      181 gaggctgaac tgcctgttga caatgggcgg tgtgaaatac cacccctcta tgcgatcagt
      241 cttattatca ggctatcttg ccgcagcgca cgcagataat gaccgcaata tcgctctgcc
      301 ttacatcttc aatactatct actatatata aattccctaa gaaccagtta catacgtcgg
      361 gtttcctgcc gaaagagtta gcttatatag tttgtccgtg caacggttaa aagtttattt
      421 aacggggaaa tcaaaatagc tattggggta tggttcgtct cttaatacat agtgccacca
      481 ttcgaggcta tatgcttcaa acccactgtt ttccatgatg gagcgcaaac gtctgcgatt
      541 ttgcgcttca ttgcatgata ttccatttgc cgcatgatga gagcgttcat ccataaaatc
      601 aaatcggctc cccattggta caagctcacc cgtgtctaat cgataaagcg taagatcaat
      661 ggcactgccg cggctatggc ttgattttga agccacgtat ccttttgaaa tcatctcagt
      721 tcggtcaata ttgggataat aactttcctt tgtcaggtta ttttccggct gtgcagccca
      781 ttgcataaaa cagtttacag cacgattagg acggtaaccg tcccatagaa gcaatccgta
      841 cccttgggta gcagccagtt cttttgcctt caaaagcgat tcagccaact cgtatgtccc
      901 tacaatgcga tttacttcat aaccgtcaac cggttttccg gtgaaattat cccaagtggc
      961 atatttagcg tcccaacgaa caccgtgtac tatttcatct aaaaaagtaa atcctatttc
     1021 catgcttatc acccctttaa cgctaatacg atcaagcggt caatcagttc gggaagtgca
     1081 atacctgcag cggccatcat acggggataa cgactgtatg acgtgaaacc gggcagagta
     1141 ttgacttcgt tcagtacaat gcggccgtta tcttgtaaaa acatatccac acgggctaga
     1201 cctctacagc cgagcgcttt atatattttt tttgccgttt cctgtatccg tcctcgctcc
     1261 tctgctgaaa ggtctgcggg aacggttata actgcgtttt cagagccttt ttccggctcg
     1321 acttcctgat gaatacgaaa gattccgtac tgcagcctga tttggtccac ctcgccaaca
     1381 gctaacgcgg cactgtttcc caataccgca caaccgacct cacagcccga aacagcctgc
     1441 tcaattaaga ttttgctgtc atattgtctt gccgattcaa ttgcgtagtc caattcgtcc
     1501 gcgctattga cttttttcac accgaaggat gagcctgaac gcgccggctt aacaaaaaca
     1561 ggataggtaa acgtagctgc caccggccta tcatctttat taataaccca aaaggcggga
     1621 gtagctatcc cagcattttt cgcaacgatg tatgtcaacg atttgtccat acaaattgct
     1681 gagctttgaa tatcgcagcc tacaaaaggg ataccggaca attcaaacag accttgtatg
     1741 gatccatctt cacctgactt gccatgcaaa gctgaaaatg ctacatcaac atggttgatt
     1801 tcatattcat ggttcttttt aacaagtaat ccgtgcattt ttttatccgg cgagagtaca
     1861 gctgaatagc aattgtcgtt ttcccattcc gcgcaaggtt tttcgcacat tttccataca
     1921 ccagatttcg taattccaat gtataacggc tcgtattttt ctttattaat gttagcggct
     1981 atctctattg cagattttac cgatacgtca tgctcctctg agcaaccccc aaacagtatt
     2041 gcaactttta ttctattcat gctcctgtct cctttcaaaa tccaaacagt ttttaatggt
     2101 tttttcaacg gtatcacgca acgcttgctc ggtataatag gccgtatgcg gtgtgattat
     2161 cacgttaggc attctttgaa gtttaagtaa aaattgatta tcaattggtt tttgggtgca
     2221 atcagagtag aaaaactctt cctctccttc caatacatcc aatgcggcac cgcccagttt
     2281 cccgttttct aatgctttaa ccaactcata ggtatctaca agtggaccgc gcccagtatt
     2341 gataagaaat gctccttgct tcattctctg tatttgttcg tggctgataa tatagtgcgt
     2401 atccgtattg agcggcacat gaagcgtaac gatatcgcta ttttgcagca actcatcaaa
     2461 cggtacatag tttacctcta tacttcggct gcgactataa gccaacactt tacatccaaa
     2521 tcctcgcagc cgctcaataa ccgctttgcc tatctggccc gttcccacca caccaactgt
     2581 catgtcgctg agtaccttgc cacggtcgct gtccaacctg aaatcatgtt tttccacaga
     2641 gcgcacaatc gattttacgt tgcgtactgc cataagaatt agcatcatag tataatcggc
     2701 aacgctatcc ggcgagtacg ccacattgtc gacagtgatg cccattctct tagcagcagt
     2761 tgtatctata tgattgcagc cgatgcttcg ggtagaaata tatttcacac cggctctctt
     2821 cagcgcaaga agaatagagg cggaaatctc tgatttatgt cccacactga tacattgatt
     2881 gaaaggcgcg gatttggcgt tggattccga cacgttggcg ttaattatcg ttgccataac
     2941 gccaaagcga ggcgaaagag catggaatgc atctgcctca tcctgctcac atccataaac
     3001 agtaatgccg atgttattca taattaagac caaccctttc tgtgaaaggc accaaataaa
     3061 acggggatgc caatggtaat cttgtcaaat ctttctctga agaacgaaaa cggctcgttc
     3121 ttacggtatt aagtctaccg tgtaagaacg agccgtttct taatattaaa ttgttaattt
     3181 cctaagaaaa agtaaagata accttgagat tttcctaaaa aattatggct ctatactttt
     3241 tgatgttggt ggatgacacc agcatctttt ttattttcca aaacatatag aaaagacacc
     3301 ttgaaatcct ttagaattaa tttggcgaac caacccaaaa ggaggaatca agatgtctta
     3361 cactcacctt atcaaagaaa cattagatat tctagactta tcagttactt ttaacgaaaa
     3421 ttgtctcaca aaagaaaaat acaaagggca aatctgtcat atttatcgtg gaaacttgat
     3481 ttatacagcc caagaatgca tccactgtaa acaccagatt gcttcagata ttgtgcgttg
     3541 gggtacaaca accgtccggt tgttaatgaa cgatgtttct gaataccgta cctacctcga
     3601 attgaaaaag caacgcttca aatgcaaagc ttgtcagcgg acctttgtcg cagatacttc
     3661 tgttgcgaaa aaacattgtt tcatcagtga gaaagtccgc tggtctgtcg tgactcgtct
     3721 gaaaaaaaat acctcaatga cagagatcgc cgctcagaaa aatctatcgg tctcttctgt
     3781 ttaccgtatt atgaagcgat tttatcgtcc gttgaatcct tttcggacac cgttacctaa
     3841 ggtactttgt tttgatgaat tcaaatcagt tcggggtgtt tcaggtgcta tgagtttcat
     3901 catgatggat ggacaaacac agcgcttatt ggacattgtc gagaaccgac aactgccttt
     3961 tttaaaacgt tattttagtc atttttcacg agaaattcgt gaagcggtcg aatggatcgt
     4021 gattgatatg tacgctcctt atgtatcgct cgttaaaaag ctgtttccaa aagcacagct
     4081 gattattgac cggtttcaca tcgtccaaca tattggtcgt acctttcgca atcatcgaat
     4141 caaagaaacg aaccaactat taaaaagcaa agaacaaaaa cactatcaat taggtaagca
     4201 attaaaaaga tactggaaac ttcttcaaaa agatgaacgc aagcttgatt atactcgacg
     4261 cctttggcga cctggcttca aagctcattt aacagagacc gatattgtgg atcgcttact
     4321 gaaagggagc cctgctttgc gtgtaggcta tcagttatat caagactttc tttacgctgt
     4381 aaaggaacga gactacgtat cgtttgaaga actacttacc aataatatca tgcttcccga
     4441 aggctatcag acgaccttga gaacgttcca gaaatttctg ccacaaatca aaaatgcttt
     4501 acaacaaagt tattcaaatg gcccacttga atgtctgaac aaccatatca aagtgttaaa
     4561 acgaaatgca tatggctttc gtagctttta taatttcaaa ttgcgcatca tgattcgtca
     4621 tggcaacgca ttgattttca actaaaaaag atcaagagga actgatccac ttgatctc
//
LOCUS       DAZKOK010000151         4604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000151 DAZKOK010000000
VERSION     DAZKOK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.154"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..417
                     /locus_tag="ACG90W_002635"
     CDS             <1..417
                     /locus_tag="ACG90W_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFQ0478933.1"
                     /translation="RFMMSIPDAARLVLSAAMNSKFGETYVLKMPAINILTLAQSLIK
                     IYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYENDKMYIIPPHYHATDELY
                     KDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            429..1433
                     /gene="neuB"
                     /locus_tag="ACG90W_002636"
     CDS             429..1433
                     /gene="neuB"
                     /locus_tag="ACG90W_002636"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0478934.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            1430..2578
                     /gene="neuC"
                     /locus_tag="ACG90W_002637"
     CDS             1430..2578
                     /gene="neuC"
                     /locus_tag="ACG90W_002637"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0478935.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            2551..3228
                     /locus_tag="ACG90W_002638"
     CDS             2551..3228
                     /locus_tag="ACG90W_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0478936.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3179..4360
                     /locus_tag="ACG90W_002639"
     CDS             3179..4360
                     /locus_tag="ACG90W_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0478937.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            4373..>4604
                     /locus_tag="ACG90W_002640"
     CDS             4373..>4604
                     /locus_tag="ACG90W_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0478938.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFI"
BASE COUNT         1698 a          600 c          906 g         1400 t
ORIGIN      
        1 cgatttatga tgtcaatacc ggatgctgca agattagttc tgtctgctgc tatgaattca
       61 aaatttggag aaacatatgt cttaaaaatg ccggcaatca atattttaac tttagctcaa
      121 tcattaataa aaatatataa tgatacacat gatgaaaaat atagaggtga aataaaagtt
      181 gtaggtatca ggccaggaga aaaattatat gaagagctaa tgactacaga cgaaattgaa
      241 cgctcatatg aaaatgataa aatgtatatc attccaccac actatcatgc tactgacgaa
      301 ctgtacaaag attatgaaaa aattaaaatc actgcatact cttcagatgc aacagaatta
      361 atgagtggac aggaatttat tagaatcgca aaagagaata atgtcgtgac ttggtaagga
      421 gttttatgat gaaatcagaa gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa
      481 gtctagattt agctttagaa agtattgata aagctaaaga atgtggagct aacgcggtta
      541 aatttcaaac atttaaaacg aataagctag tttccaaaaa ggctccaatg gcaaaatatc
      601 agaaggataa tcttaaagag gacagttctc aatttgaaat gctaaaaaaa ttagaattat
      661 ctcaatctga ttttattaga ataaaagatt attgtataca aaataaaatt gattttttgt
      721 ctactccttt tgatgaagaa agtgcagact ttctaaaaca aattggtgta caaggattta
      781 aaattggctc aggagattta acaaatttac cttttcttag aaaaatagat atgtatgggt
      841 tgccagtttt attatctaca ggtatggctg atattcatga ggtcagtgag gctgtagatt
      901 gtttcataga tagtccagtt actattttac attgtacttc aaattatccg gctagttctg
      961 aagatattaa tttattggcg attaacacat taaaagaaat gttcgggata cccgttgggt
     1021 actccgatca ttcattggga tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg
     1081 taattgagaa acattttacc ttggataagg aactacctgg tccagatcat aaagcttctt
     1141 tagatcctca ggaatttgca gattttgtaa agcatattag aaacactgaa atatttttag
     1201 gtgatggtgt taagcgagca atgccttctg aacttagtac aaaagaagta tcaagaaaaa
     1261 gcctagtggt cactgagaat ttaaatgtgg gagatattct tacagagaaa tctcttacaa
     1321 taaaacgtcc tggaaatgga atagctccta agtttttata tcaatatatt ggcaaaaaag
     1381 ttaaaagacc aataagtgca gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat
     1441 acttatagta agtggaacac gagctgatta tggaatatat aaacctgttg ctgagaattt
     1501 actacaagat aattgggatg tatcatttgt tgtttctgga atgcatttat cccataaata
     1561 tggatttact aaagaattga tcttagctga cggatttaga attagtggag aagttcaaag
     1621 tttgttttta gagaacaata agggaaacat ggctcggtct gtggcattag agataaatgg
     1681 tttcacaaat atatttgaac aaactagccc tgattttgta ttattactag gggatagagg
     1741 agaaatgttg gcagctgcga tagcttgttt gtatcttgga atcaaaacag cacatattca
     1801 cggaggcgaa gtgtctggta ctgtagatga atcaataaga catgccataa cgaaaatagc
     1861 atctgttcat tttgctgcta cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga
     1921 ttcttggaga gtttttcccg tcggagctcc tagaatagat actattttaa ataatagatt
     1981 acctagtttt agagatataa aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt
     2041 tttgcaagta tttcatccag ttgtaaccga actagaaaat atagaaaaac aaattttagc
     2101 tttgatcaat tcaatggaac aacaatctca accaattatt tgcattttac ctaatagtga
     2161 tgctggtagc gaaattatca gaaaaatgta ccagaaatat ttaaaaacgg atattattta
     2221 tattacgaat ttagagccgg aggaatacct gactatatta aaagagtgca aatttatgat
     2281 cggaaattct tcttctggga taattgaagc tgcatcattc aaaataccag taataaattt
     2341 aggaaccaga caaaatggac gagaacaatc taaaaatatt ataaatgcaa atctaaatga
     2401 aaatgaaatt gaaactgctt tgaataaaat acaagatcct cagtttataa aaagcttgaa
     2461 aaatgttaag aacatctacg gtgacggcaa cagttctaga agaataacaa aaacattgaa
     2521 tcaaatattg aatgatgggg aagatattaa gtggattcaa aaacaaatag cttattaaaa
     2581 tatactagtg atccatacat tattattggg gctggcggac acgcaaaagt cattattgaa
     2641 accttaagat tagaaaataa gttaatatat ggattttttg atgataagca aaataaaatg
     2701 caagatctga aatatcttgg gaaaataaac gaattgaata gctgggcgga tagcaaaaaa
     2761 aaaatctata aatttattgt agctattggt gactgtgtag taagaaaaaa gattataaaa
     2821 aaattgaata tgccaagtga gcaatatgga atagctgttc atccaagtgc tatagtggca
     2881 aaagaaattt caattggtta tggaactgtt atttttgcga atgctgtaat taacccagat
     2941 gctgtaattg gagagcatgc tataataaac acaggtagca ttgttgagca tgattgtaga
     3001 attaataact atgttcattt atctccaggt gtatgcttag cgggtggagt acatgtaggt
     3061 gtaggaactc aaataggaat tggttctcaa tgtatccaga tgaagaaaat tggatcttgg
     3121 tgtatgatag gcgcagggag cactattgtt aaggatattc cctcacattc cttagcttat
     3181 ggcaatccag ctaaaattaa gaaagaagga attaattttg aattctagaa ttttattatc
     3241 aagtcctcat atgggaggaa cagaagaaaa atatgttgct aaggctttta aagataattg
     3301 gatagcgcca ttaggtgaaa atgtggataa atttgaagaa agtattacaa aatatactgg
     3361 ttctaagggc ggagcttctg cgttaagttc aggtacagct gccatacatc ttgccttaag
     3421 actgcttaat gttggaaaaa acgatattgt ctttgctcct tcatttactt ttatagctac
     3481 agtaaatcct attttatatc aaggggctgt accagtgttc attgactcag aacctaatac
     3541 atggaatatg agccctgttg cactagagag cgcattagaa gaagctgaaa gaaacaacca
     3601 attacctaag gcaatcatcg tagtgaattt atatggtcaa atggctgatt atgaacgaat
     3661 tttagctatt gcaaaccaat ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg
     3721 ttctacttat aagggaattt cgtcaggctt atttgggaca attgggatct attcatttaa
     3781 cggtaataag atcatcacaa cctcaggtgg aggagctcta gtgtctagtg ataaaaaata
     3841 tgttgagcat tcaagatttt tagcaacaca agcaagagat caagcaccgt attatcaaca
     3901 tagcgaaata ggttataatt accgtctaag caatatttct gccggtatcg gacgtggaca
     3961 aatggaagta ttgaatgata gagtagctaa acgaagagaa attttcaatc gatataagga
     4021 agctttttct ggaattgata gaattgaaat gatgccagaa gtggatggca tggtgccaaa
     4081 tcgttggttg tctactttaa cgatcaatga ttataattct attgatcaag tagttaacgt
     4141 aataaataaa ctaaatgaaa aaaacattga aacaagagct ttgtggaaac cactacattt
     4201 gcaaccagta tttcaagggg taaaaatgta ttaccatgat aataatctta gtgtgtcaga
     4261 aaagttattt aatagcggct tatgcttacc ttctggaagt aatatgacag atagtgaaca
     4321 agaatttgtt attgatgagt ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat
     4381 aggaattatt ggtttaggtt ccatggggaa aagaagactt agactcattc aagaacactt
     4441 taatgatatt ttactatgtg gtgttgatac taatgaggcg agacgaaagg aagttgaaaa
     4501 ggaatttgga ataccaactt ataatagtct aacagaggca aaaaatttgt ttgaaataaa
     4561 ttcagttttt gtttgtacat cccctataac ccatgaattt atta
//
LOCUS       DAZKOK010000152         4447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000152 DAZKOK010000000
VERSION     DAZKOK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.155"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG90W_002641"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG90W_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0478939.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG90W_002642"
     CDS             complement(2020..2922)
                     /locus_tag="ACG90W_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0478940.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG90W_002643"
     CDS             complement(2919..3611)
                     /locus_tag="ACG90W_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0478941.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG90W_002644"
     CDS             complement(3654..4295)
                     /locus_tag="ACG90W_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0478942.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1377 a          948 c          768 g         1354 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagatt
//
LOCUS       DAZKOK010000153         4433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000153 DAZKOK010000000
VERSION     DAZKOK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.156"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG90W_002645"
     CDS             complement(543..2048)
                     /locus_tag="ACG90W_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0478943.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG90W_002646"
     CDS             complement(2045..2731)
                     /locus_tag="ACG90W_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0478944.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG90W_002647"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG90W_002647"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0478945.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1545 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAZKOK010000154         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000154 DAZKOK010000000
VERSION     DAZKOK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.157"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG90W_002648"
     CDS             690..1658
                     /locus_tag="ACG90W_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0478946.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG90W_002649"
     CDS             1726..2484
                     /locus_tag="ACG90W_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0478947.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG90W_002650"
     CDS             2552..3262
                     /locus_tag="ACG90W_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0478948.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG90W_002651"
     CDS             3298..>4425
                     /locus_tag="ACG90W_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0478949.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKOK010000155         4396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000155 DAZKOK010000000
VERSION     DAZKOK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.158"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..93
                     /locus_tag="ACG90W_002652"
     CDS             <1..93
                     /locus_tag="ACG90W_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0478950.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="ACG90W_002653"
     CDS             complement(151..1371)
                     /locus_tag="ACG90W_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0478951.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="ACG90W_002654"
     CDS             complement(1391..2002)
                     /locus_tag="ACG90W_002654"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0478952.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="ACG90W_002655"
     CDS             complement(2045..2977)
                     /locus_tag="ACG90W_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0478953.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="ACG90W_002656"
     CDS             complement(3398..4258)
                     /locus_tag="ACG90W_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0478954.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DAZKOK010000156         4377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000156 DAZKOK010000000
VERSION     DAZKOK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.159"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG90W_002657"
     CDS             73..234
                     /locus_tag="ACG90W_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0478955.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG90W_002658"
     CDS             253..1530
                     /locus_tag="ACG90W_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478956.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG90W_002659"
     CDS             1540..2196
                     /locus_tag="ACG90W_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478957.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG90W_002660"
     CDS             2196..3572
                     /locus_tag="ACG90W_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0478958.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACG90W_002661"
     CDS             3702..4115
                     /locus_tag="ACG90W_002661"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0478959.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACG90W_002662"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACG90W_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKOK010000157         4307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000157 DAZKOK010000000
VERSION     DAZKOK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.160"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..980)
                     /locus_tag="ACG90W_002663"
     CDS             complement(366..980)
                     /locus_tag="ACG90W_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0478960.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1586..1963)
                     /locus_tag="ACG90W_002664"
     CDS             complement(1586..1963)
                     /locus_tag="ACG90W_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478961.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2263..3144)
                     /gene="galU"
                     /locus_tag="ACG90W_002665"
     CDS             complement(2263..3144)
                     /gene="galU"
                     /locus_tag="ACG90W_002665"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0478962.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3187..4206)
                     /locus_tag="ACG90W_002666"
     CDS             complement(3187..4206)
                     /locus_tag="ACG90W_002666"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0478963.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         1267 a          915 c          671 g         1454 t
ORIGIN      
        1 aataatgata acaaatgtac tataattact ttgtaaagag ttttggggaa gactctttca
       61 tcaacgtgta ttaagagaca agaaagaatc catatggatt ctttcttgtc tctttttcca
      121 ctacttttta tttttctgac actacatatt cttataactc atttctcata actgtgaaac
      181 aacttataaa aataacaaac tttaaacaaa acaagcttaa aatcaaagat gatccatctt
      241 aaaataacta aaacaaaaaa agtagtcgtt gttaatcctc attaataatc gttttctttt
      301 tttcaaacgc tccattttta tgtggaacat tattttttta aggaactttg tcaacatgtg
      361 gataactatt ttccacttgt gtcctggact agctgttttg ctgtaaaata caaaacttgc
      421 tgcagcaaaa agatcgttgc aagagaggct gttccgcaga tcgctggatc tagatttggc
      481 aaagtcaaga ctgcatcatg taaattggcg tggttgtcca aaaccaaatc agcaaattca
      541 aataatcgca aaccactgct atgttgcgat ttgatacttc gtgacaaatc aaatccagta
      601 acaatgatta atggatgacc attttctttg atccactgag ctagttcgat gattgccggg
      661 tttcttcctt cattcgaaag aagaagaaaa atgtcttctg atttgacaga aagctgatca
      721 ataattgcct gtcctgcacc ttctagcgtt tcatagacac ctccggctgg attttcaatc
      781 aatatacttg gtacaagatc cggggtttga tcccaaaatt caaatgctac accacttagt
      841 gtgcggctag catatatctg caatctacca ccttgattaa tttgtttcgc gatccaagtg
      901 ccagcttgaa gcatcagcgg ctcttctatc cttaatttct cttccaattg gtgataatag
      961 tcaaaataac ataaactcat ttatctctcc cgcctagcta tacttgacat gaatagaaac
     1021 agctgcctaa tctaaggaag tacataaaaa aacagtagtt agacagtaac acatgtggtc
     1081 tttgctgttt caaacacaaa aaaggcattg aacgaatacg cccaatacct tgacatagca
     1141 aataaaacaa cgtgcagacc atggaaaata tctacatagg ggtagaattt tcaccctaga
     1201 ttaggtacaa taaattaggt cgatgcactg cattgactat ggcaaaatga tcggtcaggg
     1261 tgtatgtgtg tgactggtca ttccctgatt cttagcgcca actaagggtc agggagcctt
     1321 tttttattca gctgtttcta tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc
     1381 aagaaaatac aaaaaaacat tggtatgatt tatatatata taaataacat aaaaaaagga
     1441 atggcttatc ccgatacaac aagcattcct ttaaaaaaga ttcttttata taagtgaaaa
     1501 aagctttttt ccaccaatta aataaatctc tcatctcaat agtttatcca ttattttatc
     1561 tttttttgcg tatacctact aaatattacc gtcttcttct cgttcttcta ctttgttcga
     1621 gagaatctgt actagaaaaa gtagttgtat gtctaccttt tctgcctatc cgagactttc
     1681 ttttcatata tgggatataa atgattagat acagtacagc aaaaaataaa gcaaatacgc
     1741 aagcttccca actacctaac gccaattcaa ccatcaaggt agcaacccca acggatgctg
     1801 cataccagat aaaaatcaga aacgtttctt tttttctgac catgcgaaac atgataaata
     1861 gactaataag caatagtggc aacacaaata tccaattgct tccttccatc agccacttga
     1921 gtatatcaag cagcgactgt gcaacagcga ctaattttcc catagtgaac acacaccttt
     1981 ttttataatt aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat
     2041 ttctcttatg taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa
     2101 tattttctga atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg
     2161 atctagagtc aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa
     2221 taacaaatag cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag
     2281 ctttttactc aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag
     2341 tccatattca atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc
     2401 tttaaactct ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag
     2461 ctgtatctcg tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt
     2521 cagtagataa cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc
     2581 tacaaaccct tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc
     2641 gtatttagat gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc
     2701 ttcataatcc atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc
     2761 cagcatgacg acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc
     2821 cagtcctctt ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac
     2881 taaagcgagt aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc
     2941 atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat
     3001 tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag
     3061 catttctttt gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg
     3121 aatgactgct tttttgacct tcacttgctg ttcctcctaa acgacgatta actattgatc
     3181 atctatttaa cgaagagaaa attcattctc catttttccg tcacgtaaca tgatttcttt
     3241 tgctgctttt ttgatatctt ctccattata caaaacatta tagattgttt gagtaattgg
     3301 catctcaaca cctagctgtt gagctagctc tactgctgct tttgtcgttg ccacaccctc
     3361 aacgaccatt cccatgtttt ctagaacttc ttccagctta tgtccctgtc ctaacaagtt
     3421 accggcacgc caattgcgag aatgaacact tgtacaagta acgatcaagt cccccacacc
     3481 actcaaaccg ataaatgtca gaggattggc acccatcgcg acaccaagac ggctgatttc
     3541 tgccaatcct cgagtcatga ttgctgcttt tgcatcatct ccgaatccta atccatggat
     3601 agcgccggca ccaattgcaa tgatattttt cagtgctgca ccagtttcta cgccaatcac
     3661 atcattattt gtataaatcc tgaaataatc attcatgaac agtttttgta cataggctgc
     3721 tgctttttga tcaatacttg cagctgtaat tgttgtgata tcatgtactg ccacttcctc
     3781 agcatggcta ggaccagata atacaacaat tgcctgtctt ttttcttctg gaatttcttc
     3841 agccaaaact tctgagattc gtttatgcgt atcttgttct agtcctttac ttgcatgtat
     3901 aatgattggc ttagtattca ttttaccaat aagttcctgt gcgactgaac gaatcgcttt
     3961 tgttggaaca acaaataaaa tagcatctgc gttatccact gcttctgcca attctttata
     4021 tccgataatc gattctggga tcgtcaaatc aggtaaaaaa tgctgatttg tatgatactt
     4081 attgatttca tcaatctgtt caggtacatt tccccaaata cggacatcat gcccgttttc
     4141 tgccaatgtt tgtgctaaag cagtgcccca agatccagga ccaagaactg cgattttttg
     4201 tttcatggat attcctccaa ataaataatt gataatttcc attatagtat acatttctta
     4261 aagttgggac taatttccat tgtttttaag tgtttgttta cttcttt
//
LOCUS       DAZKOK010000158         4305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000158 DAZKOK010000000
VERSION     DAZKOK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.161"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1137
                     /locus_tag="ACG90W_002667"
     CDS             148..1137
                     /locus_tag="ACG90W_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0478964.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="ACG90W_002668"
     CDS             1134..1553
                     /locus_tag="ACG90W_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478965.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="ACG90W_002669"
     CDS             1562..1870
                     /locus_tag="ACG90W_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478966.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="ACG90W_002670"
     CDS             1874..2236
                     /locus_tag="ACG90W_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478967.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="ACG90W_002671"
     CDS             2458..3864
                     /locus_tag="ACG90W_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0478968.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DAZKOK010000159         4295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000159 DAZKOK010000000
VERSION     DAZKOK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.162"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG90W_002672"
     CDS             <1..792
                     /locus_tag="ACG90W_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0478969.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG90W_002673"
     CDS             889..3138
                     /locus_tag="ACG90W_002673"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0478970.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="ACG90W_002674"
     CDS             3141..>4295
                     /locus_tag="ACG90W_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0478971.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DAZKOK010000160         4293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000160 DAZKOK010000000
VERSION     DAZKOK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.163"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..1596
                     /locus_tag="ACG90W_002675"
     CDS             172..1596
                     /locus_tag="ACG90W_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0478972.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1777..2550)
                     /locus_tag="ACG90W_002676"
     CDS             complement(1777..2550)
                     /locus_tag="ACG90W_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0478973.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2547..3452)
                     /locus_tag="ACG90W_002677"
     CDS             complement(2547..3452)
                     /locus_tag="ACG90W_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478974.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3606..3926)
                     /locus_tag="ACG90W_002678"
     CDS             complement(3606..3926)
                     /locus_tag="ACG90W_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0478975.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1310 a          769 c          852 g         1362 t
ORIGIN      
        1 ttttaagttt tttggtggtg taatttggaa agggactagg cgctttgctt gtataatgag
       61 aaaaaagccg gtagaattat atttctatta aagtttttat taattaagta aaaataaaaa
      121 aggtttgtta tactttttga taaaagcggt tttattttgg aggatgctta catggataca
      181 gagcaacaga aaaaaggaat tggtttaatt ccacttgctg ccttagtaat tggttcagcg
      241 attggaggtg gcgtatttgg tattatgacg gacatctccg gatcggctgg cggcccagct
      301 attttcagtt gggtactcgt aggaacagca atgttgatgc tctccttgtc gatcaataat
      361 atcaataaaa aacgtccaga tttaaaaggc gggatcttta gttacgcaga agaaggtttt
      421 ggccgtttcg ctggttttat cagcggttgg ggttactggc tcacatgctg gttaggcaac
      481 gtagcttttg caacattgtt gatgagcgca ttaggatatt tcttcccagt atttgaaggt
      541 gggcaaaatc tggcatcgat cgtgatgagt acagtctttt tatggggata tgcatggcta
      601 gtgaatcggg gagtcgaaaa tgctagtttc gtcaatgcta tcgtcacatt ctgtaaatta
      661 gtgccattat ttttatttat tgtagtagct atcagcacgt tttcgatcca acaattcatt
      721 gataactttt ctagcgacat tatattgaat gcgaatggac agccgatccc gttttccagt
      781 caagtattga gttgcatcat ggtgatgctt tgggtatttg ttggtattga aggagcctct
      841 gtcgtaggtt cacgagcaaa aaaacaatca gatgttggga aagcaacgat tctagggata
      901 ttcggcttga ttattattta cgtcttggtt tccatgcttc catatgggac aatgacgatg
      961 aatcagctgc aaacgatcaa ggcgcagcct gctatgggct atgtgtttga aatgatggtt
     1021 ggtaaatggg gagcagtcgt gatcaacggc ggtttgatca tttctttagt tggtgtttgg
     1081 ctctcatgga cgatcttacc gattgaaaca atgggaaaca tggctgaaga cggacttttg
     1141 cctaaagcat ggggaaaact caacaaaaat ggggcaccaa cttatgctat cgtattgacg
     1201 acgttatgta cgaatgtctt tttactttca ttattattca ctgacagtgc ttataacttt
     1261 gcttacacac ttggtacagc agctattttc ttcacgtggc ttttcttagg attgtaccaa
     1321 atgaaacttt cttatcagcg gaaagaatgg gtccaatttt tagtagggct tttagcaagt
     1381 gcctttcaag tatgggctat gctagtcgta gcctttaatg aagtaatgct tgttcttgtt
     1441 cttttcttgc caggcattta tttttacctc aaagcacgta aggagcaaaa cgagcctgta
     1501 caacatatcc acttagataa gattttactt ataggtgtga cgatggtcgg agtgattgca
     1561 atggttttat ttgccactgg gatgcttcac gtatagaagt ataaaagaag tataaagaga
     1621 ggttgtgagc ctgtcgttta ttcaaaaaca tactaggaaa ggagctgtga cacaaatgtt
     1681 gtcacagctc ctttttcaga ataaacggtg tttaagagac agccaatcgg caataaggct
     1741 gaacgtctct ttcacagcct cttttttgat gagtcatcat acattttttc gcttaaatac
     1801 gagatatcct aatgctaaga acaaaacgat gtaagctaaa ttaccgaata gcatttgatt
     1861 agtagaaaga tgagtcaatt gcttcatgat ctcttcgttt ccaatttggt tttgcagatt
     1921 caacatactg atcggattcc atttgatcca ctcccatttt tggatgagtg caataaggat
     1981 accagatatg attgacgaag caaaataaaa gacgattcct gctgagatgg atgcgccgga
     2041 actattgata aagcaagcaa tcatcaacac taaacttaaa atcagccata aaccgatata
     2101 actacccaaa gtataagtga acaagctttg gatcagtgtt tgaccagtag aaaccttctc
     2161 cgtgaaggaa atcgaaggga aaagaatcag tttcatcaac acagtgacaa taatagctaa
     2221 aaggtataaa taaacagaat aaataactaa tgtgatccat ttgcttacta atatttctcc
     2281 tcgagaatac tttcgataaa ggagattttt caatgtacca tattgtgctt ccatcgaaat
     2341 gatcgtgctt gcagctgcaa tcatgataaa gacaatccaa gatgtggcac taaaaagctg
     2401 agcagtcatt tcaactggat caatgatcgt cgtgtatttt ttggctagaa gtgctgttcc
     2461 tatgagcaaa acaactaata gaacagataa aacagcagtt gactttttct tgatcaattt
     2521 aaataattct tgttttacaa gcaatgtcat ttttcttctt ccccctttac tgaatccaga
     2581 atttccaata gagaagattc caaatcattc gtcagatgct ggacgtctgt cacatggata
     2641 ttttgtgcag acaatagctg gatcacggtt cctaataatt cttcgttttc ttttaaaagc
     2701 ttgattttat caggatcttc caaagtatat ccagcttctg tcaagatcgt tcgagcagct
     2761 agatcatttg ttgtagagat aacgatgaaa tcttgcgctg tttggttcaa ttcagccatt
     2821 gtacattgtt tgatgatctt tccttgattg atcaggatca gctcatcgac cattttttca
     2881 agctcgctga gtaagtgact ggagatcaag aaagaggtgc cctgagtagc caagtcggta
     2941 atgatttttc gcacatctct tgttgcttgt ggatccaaac cgttcatcgg ctcatctaaa
     3001 ataacgatag acggatggtt cagtaaggca agagcaattc ccaatttttg tttcattccc
     3061 aacgagtagg atttcgcttt tttgtgaatg tacttttcca tcttgagctg attcacgata
     3121 gtgtcgatag cagatacatc gttggtttca gagaataact tcagatgatc atagcctgtt
     3181 aagaatggat agatacccgg gtattcgatc aatgctccta cttgttccaa ccctttatga
     3241 ctagagggag aaatctcatt ttcaccaata cagattgttc catctgagta atggatcagt
     3301 cctaaaatag ctttcatgat cgttgttttt ccagcgccat taggacctac taatccaacg
     3361 attttgccag gggataagct aaaactgaca tcgctgaggg cttgtttgtg accaaagttc
     3421 ttggataatt cttggaccga aagacttttc aaaaatattc actccttttc tatagatagt
     3481 acaagcatac acctgatgag tggagaagtc gctaggtcat tggacttatt tcttattttt
     3541 tcataaaaaa attgaatatt aaataaaaag gcttattttt tctgatcagt acttattcca
     3601 agtgtctagt ggccgctagt aatcttgatg ccaatgatac tgatgagtag catccccaca
     3661 aagaaccaag tggaaagatt cagcttatca tgaaacaaga ccacaccaat tatgaccgaa
     3721 ccaactgcgc caatccctgt ccagatagga taagagagac tcatcggcaa acttttgatt
     3781 gcctttgaca aaaagtaaaa actagcaatc atgccaatca ctgtgtaaaa agaataattg
     3841 attttagaaa aaccatcact ccatttcatc atcgttgacc agaatacttc caaaattcct
     3901 gctatgatta attccatcca tgccatactt acattcctcc ttaactataa aaaaatacac
     3961 aaaaagagga aaatcatccc ttcaaagacc taatggaatg atcatcctct ttatcctacc
     4021 cggtggcaga ttgtatatct ttttctgtag tatagcacac ctatccagat atcggcaatc
     4081 tcatactttt tagtaaagtg taccaagcac tgacggatat atcgttagcg gaaaagaaga
     4141 tgaatgcttt gcaatttcat agaaagaagt tgttatttat taatcattta aaaaaaacag
     4201 taactagacc ttatttctat atattatcgg gcaataaatt aaaaaaagct ttaccttaca
     4261 ataagtaaga taatttttgt gataaaaaaa taa
//
LOCUS       DAZKOK010000161         4246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000161 DAZKOK010000000
VERSION     DAZKOK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.164"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1873)
                     /locus_tag="ACG90W_002679"
     CDS             complement(107..1873)
                     /locus_tag="ACG90W_002679"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478976.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1833..3587)
                     /locus_tag="ACG90W_002680"
     CDS             complement(1833..3587)
                     /locus_tag="ACG90W_002680"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0478977.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3697..>4246)
                     /locus_tag="ACG90W_002681"
     CDS             complement(3697..>4246)
                     /locus_tag="ACG90W_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0478978.1"
                     /translation="FIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFV
                     YISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIP
                     QVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVL
                     VVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1437 a          803 c          597 g         1409 t
ORIGIN      
        1 ttctttcgtg tattcaaaat tagatgctgt ttaataaaat tattcttttt gaaaaagaat
       61 tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat cgtgattgag
      121 cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga tttttgtata
      181 tttctgacct tcgatacaac tcattatgag taccttgatc aactagtttt ccttgatcca
      241 tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata attagtactg
      301 tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt tccgcatcta
      361 aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta
      421 tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg accaatgtat
      481 gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga gctgcttttt
      541 tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa atacttgtat
      601 taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa gctggagata
      661 cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca tacagtctag
      721 acactaattt caagatacta gttttcccgg aaccactagc acctactaac gcagtcactt
      781 ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta tcatatgaga
      841 aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat ccctcttgga
      901 tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa aatatttcaa
      961 gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct agtaagtaac
     1021 caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct actagaatga
     1081 ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa aaagtactta
     1141 attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt ctgtcttgaa
     1201 cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt tcttgaaatt
     1261 cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt tgggatttct
     1321 ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct aatcccattt
     1381 tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc ataccgccgg
     1441 ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac atgatagttt
     1501 gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt gacaaatcgt
     1561 ctgcaatttg cactcttaaa tgcgcacttt ctttataagt actattatat aagttttcat
     1621 actccctatc taacaaccaa tacatgaaaa gcaaagtaac aaacgatacg gcaaaataaa
     1681 gccaacgggg ctttgtattt tctaaaacta attcgtctat cagcatcatt ataatgaatg
     1741 ctggtaccat attaatgcag tatacaaaaa aactagaaat agaagcttta gttaaagctt
     1801 ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa acttttcatt gtcaagcctc
     1861 caatcatttg cattctcata attagtgacc aattgtttat acacttgatg tttagccatt
     1921 aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag aatttcatct
     1981 acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc tttcatcaga
     2041 tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc actggcttca
     2101 tccataataa caatctttgc atcttttaaa attgctctgg caatagctat acgttgtttt
     2161 tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct ttcgggaaat
     2221 ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt acttgctggc
     2281 gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag tttcgtatct
     2341 tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt taaaggctta
     2401 ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag tttagctatc
     2461 gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg ttctaaaact
     2521 aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt ttcaaatgca
     2581 atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg cttcatttct
     2641 aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg catacttaca
     2701 tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa catcaacaag
     2761 tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat aggaactatc
     2821 aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt tttcccactc
     2881 aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa ggctttaaat
     2941 gatgttactt tagcaccaaa tatctttata acttgaattc cgcggatata ttctaccgtt
     3001 tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt attctctgtc
     3061 attttcttta acaagaatac accaattact aacatggcga ttagaaccaa tccaatttta
     3121 aagttaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc ttgagcacta
     3181 tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc aattgtcttt
     3241 cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta
     3301 attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga aaataagcca
     3361 gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac cgcataactc
     3421 ttagctaaat attcatctcc tacaataatt agagactgaa gaaatttgta aatactgtaa
     3481 tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa ccatccgtag
     3541 tatttcattt tagggacata actaaataat tttttgtata ctttcatttt ttcctccccc
     3601 gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat
     3661 tagagatgta caaacagata aaaggtaaac tacgttctat tttttgtatt tttttaagac
     3721 ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca ctgaaatgat
     3781 tccccatata cctaccgcat agtggatcat tgcatccatt gttgcagcag attgtccttt
     3841 atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc actgtccggc
     3901 agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc gatcatatcc
     3961 aaatacagat agaataatat cagctaagat cgccatacta atcgaactga taaaccaagg
     4021 taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa
     4081 aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag ataataatcc
     4141 aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca atactactaa ccattattga
     4201 aaaaaagtaa attactaggg aaataactgt agctaaacca ataaaa
//
LOCUS       DAZKOK010000162         4240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000162 DAZKOK010000000
VERSION     DAZKOK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.165"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..477
                     /locus_tag="ACG90W_002682"
     CDS             40..477
                     /locus_tag="ACG90W_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478979.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            572..1456
                     /locus_tag="ACG90W_002683"
     CDS             572..1456
                     /locus_tag="ACG90W_002683"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0478980.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1678..>4240
                     /locus_tag="ACG90W_002684"
     CDS             1678..>4240
                     /locus_tag="ACG90W_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0478981.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1387 a          712 c          959 g         1182 t
ORIGIN      
        1 aagaaaacga tattaaaaaa tgcacgtaga agtagataaa tggagtggtt ttgcaaattt
       61 cttttatttc tagaagttat tcacgtatac ggatttgttt ttctcacaaa taaaaaggca
      121 ggaagtacaa tggatcattt gtatataaaa tatattcatc aattttccgt tttggtatta
      181 ggcacactaa ttatagcggg gattgttgca tttattattc aaggtcgtga catattaaca
      241 gtacatcaag agtttttcag ttatgcattt ggtattcctc tgatcgctat aaggtctgat
      301 aacaaaaagt ggaagaaacg ctattacatt ctcttgctat ttttgtgttt ttatctgcca
      361 cctaagattt ttactttatt aggtatacaa tgcctatcat tttcttttat ttcctatatc
      421 attgttttaa ttttcctcag tttcatagta tgggtatttt atcttgtcaa aaaataaatg
      481 aatgtttcat gaacagtact tcatcacagc ctaatttttt tctttatgct atcgttatat
      541 taaagaaatc aatcagaagg gaaagaaagt gatgaaaaaa gttttattag tggtaaaccc
      601 cagttcaggc ggtgaacaag caaaggaatt tgagcaatta gcgatagcaa aattggaatc
      661 ggtgtttgat gaagtggtcg tcttgcatac aaaaaaagca ggggatgcaa aaaactttac
      721 tcgcgaagcg gctacggaag ggtatcatag tgtgtttgtc atgggcggag atggaacagt
      781 caacgaagga atcagtggaa tcgctgaaca agaacatcgg ccaaatttcg gatttttccc
      841 attgggtacg gtgaatgact tggctcgagc attagggata ccgctagaac ctgaggaagc
      901 gatcaatcat ttttcgattg aatcagtcaa gccattggat attggaaaaa taaatgacga
      961 ctattttatg aatgtcgtcg caattggctc tatccctgaa gcaatcaatg atgtagatgc
     1021 ggagaagaag acgaaatttg gaaaactagc ttatttcatg tcaggaatca aacagttagc
     1081 ttccacacaa tcctactcgt ttcacgtaga agttgatgga aagaaggaag aaattgagag
     1141 cagtatcttg ctgataggac taactaactc agtaggcggt tttgaaacat tacttccaaa
     1201 tgcaaaagtg gatgatggta agctccattt ggtttattta aaagattctt ctttgatcga
     1261 tacgcttaaa acattgccag acctattaaa aggagtagat gaatctacga aaaatctagc
     1321 ctatcttact tgtgagcaat tagttgtgtc attagctgat gaggaagaat tgacgacgaa
     1381 tgttgatgga gatgaagggg ataaattacc tgtgacaatc aaagtgttac cctcacattt
     1441 acaggtttat tgttaaaatg gaggttgtga ccgaagagtt gtcaggagga catcggttgt
     1501 tcggaataag ggactgtgac aagacttttg tcacagcctt tttttgtaca aaaaagaaat
     1561 tttcacctaa cagaaaaatt tgccataaaa atacatattt taaatatgaa agtacaatta
     1621 caaataaatt aaaacgttaa aataaaattg tcaaaacgtc agagaggatg gtggagaatg
     1681 aatcaatatg taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg
     1741 acatccgttt ttgccgatgt agagaaaaca agcgaatcaa cgcaatcaga gaatcaaagt
     1801 tttgacaaga ctactttttc agaagaatca attacttctg aaactacaga atcccaaagt
     1861 gaggcgccta cttctaatac aaggcaagaa gaaaataaga caagtgagac ctcagaagta
     1921 gctgaacaaa cgtctgatag cagtgaagag gtacaggtta gagttgtttc agcggatggg
     1981 tacagcgaag ctgccacaac agaagaactg acgcagcttc tcgcagacga atcagtagct
     2041 aagattcgtt taactcagcc tttgacttta gacagggaat tggagatcaa gcgagatatc
     2101 gttatcgatt ttggcggact tgctcataat ttcggcacac atcacatcta tatcaatgag
     2161 acacctcagt cgatcgagtt tcagaatttt aaaggaacgg cagctcatcc aggtgggttg
     2221 ataccaaata tcgatggaaa tgccattatc attgcttata tgtccgattt ttggggaaac
     2281 agatatcatt ttacgggaga aataaaattt accgggacac ttgacttata tgatggttct
     2341 aaattaggcc tgatttacgc ccctcgtgca actgtcacat tagatggtgt ttccggtgta
     2401 ttggatgtcc aaccagtgaa agaagggatg aatgccgctc cgggtaaagc ttatttttgt
     2461 agaagctatc agctaaatgt aatcaatggt agtcagcttt atggaccata tctaggaaaa
     2521 ttctatggtt ttctagattc aggtgatgag acgggcagtg gaaaaacagc accaggactc
     2581 catatcttga gtggttcaaa ggtctctctt gactatgatc gggcagatgg cgtcagcgat
     2641 gaaggagagg ctgtcgatac gctacccggg aatgtcacat ttaaagtcag tggctcagga
     2701 agtgaatttt cggtaaatac aaagatcaat attaccaacg acaacaatcg cggaattatc
     2761 caaatgagag gctcaggatc aagagtcgct gtgtcgaatg atgggaaaat cacaatcaat
     2821 acagcgacta ctggcggatt tcgcctgcaa ggagattcta gtcaaatcca tgtatcaagt
     2881 ggtggacaaa tctcggtcaa aatggctgga gatggcgacc aacctggaaa caatgggatg
     2941 cgttttgtag ggaaaggact ttctttgatc gtagaaggag ccggatcagt catcgatatt
     3001 gataagcaaa gtggtcgttc tagcgcagtt cgttttgaga acggtacgca aaaactaaca
     3061 gtaactaatg gaggctcatt gaaggtaaga aatgcagggg atggcaaatc ttatgtgaac
     3121 agactcaatc aaggaaacca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     3181 ccagggtcag ctgattttac ggttactgga gaaaaatcaa atatcgatat acgcgcagat
     3241 tccggagcta ccgtagatac aactggagat attgatttga actttatggc tggcgaaaaa
     3301 acttatttag tcatggtcgg gaaaactggg gaagataaga ctggcatcat cagtggggat
     3361 gtgttgaatg tagacttaga atctcctacc tatttcgatt tccaaaacaa acggatcgga
     3421 agcgagacta atggcatagg tggctgggta tttcaaggaa atggcgattc ttctttgaag
     3481 atcgctcatt cagaaattgc tttatggcga aaggcaggag taacgttcga taactttgaa
     3541 aaagatccgg attatttttc tgatatgaca gacttatcga tcaatggaac cgacttaggt
     3601 aattttgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     3661 atgaaaaatt ataatcgaat cagtgccaat aaccagcttc cgatgattga atccttaaga
     3721 gtaccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     3781 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttatgca
     3841 gatacgacga aatcacctgt agaaattact gctctcacac aaggacaaac agatgaaaat
     3901 gaagggacag gatataatcc gtggggagaa ggcgaacaag gcggtttgtt tgaggttcaa
     3961 gtacccaacg aagaaagact aacaacgggg gatcaagtgc aaatagttgc tgctaaaaaa
     4021 gtaagaggcg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     4081 cctcctgagc ctgctaaatt aagtaccaca ttgatcaatg cagcttctcg ctcaatcaca
     4141 ggtacaggcg aagcaggagc tttggtttca ttgaaaaaaa atcaagctgt tttgtccgaa
     4201 acaactattg atgatacagg taaatttgtt ctttctatcc
//
LOCUS       DAZKOK010000163         4225 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000163 DAZKOK010000000
VERSION     DAZKOK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.166"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG90W_002685"
     CDS             75..503
                     /locus_tag="ACG90W_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0478982.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG90W_002686"
     CDS             490..732
                     /locus_tag="ACG90W_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478983.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG90W_002687"
     CDS             complement(1032..1247)
                     /locus_tag="ACG90W_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478984.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG90W_002688"
     CDS             1354..1668
                     /locus_tag="ACG90W_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0478985.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG90W_002689"
     CDS             1772..2950
                     /locus_tag="ACG90W_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0478986.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(3131..3316)
                     /locus_tag="ACG90W_002690"
     CDS             complement(3131..3316)
                     /locus_tag="ACG90W_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478987.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            3353..3646
                     /locus_tag="ACG90W_002691"
     CDS             3353..3646
                     /locus_tag="ACG90W_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0478988.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3734..3880)
                     /locus_tag="ACG90W_002692"
                     /pseudo
     CDS             complement(3734..3880)
                     /locus_tag="ACG90W_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1446 a          632 c          821 g         1326 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcct
//
LOCUS       DAZKOK010000164         4182 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000164 DAZKOK010000000
VERSION     DAZKOK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.167"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..366
                     /locus_tag="ACG90W_002693"
                     /pseudo
     CDS             17..366
                     /locus_tag="ACG90W_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(638..928)
                     /locus_tag="ACG90W_002694"
     CDS             complement(638..928)
                     /locus_tag="ACG90W_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478989.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1330..1566
                     /locus_tag="ACG90W_002695"
     CDS             1330..1566
                     /locus_tag="ACG90W_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478990.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1573..3525
                     /locus_tag="ACG90W_002696"
     CDS             1573..3525
                     /locus_tag="ACG90W_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0478991.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3597..4094)
                     /gene="dfrG"
                     /locus_tag="ACG90W_002697"
     CDS             complement(3597..4094)
                     /gene="dfrG"
                     /locus_tag="ACG90W_002697"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0478992.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1423 a          736 c          714 g         1309 t
ORIGIN      
        1 tcttttaaat acttaattgt taaatacctt atttgatgca aaagaaatct acgatagtca
       61 acaaaaaaat tttcattata tccagaaaaa tttttcttag ttaaggacct atggattgca
      121 gtaatgcaga attcgtcgaa taaactgcca aagacataca atcatattgc ttttaaaatt
      181 gacgaacaag atatagattc gtttgtctca aaaatccaaa tgttaggttt aactgtcgaa
      241 ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt acttttacga ctatgataat
      301 cacctcttcg agttacatac cggtactctc caggaacgtt taaaatccta caattccact
      361 acctgagact agtaatgact agtctctttt tatggcctct gatcccccca ttgagcgaat
      421 actaataggg cttcgaattg atctgttcaa tctttaagca atatccctca aatgagtttg
      481 gaaagtttag tgattcatca caaatactta aatacatgta gttatatata gattgaggtt
      541 cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc tgcatccatc tttccaaaac
      601 aagcggctag gacatttttg tctcagccgc ttgttttcta gtttattctt tcatcttgtc
      661 ggaccttttt tcttgggtat agacttttct catattctac cacaattttg ggtttcccta
      721 acacatgata ggatctcaca acaaattgct ttctattatt ataataacct gtaacgacta
      781 acttcatccc ttcgtctact tcagctagga agttcaaact atgggaagca atcaaacaat
      841 tgaccccatt taaactgaaa cggatcaacg gtctggcact aacttgcaaa gtacggatct
      901 tatctactgt tccatatttt ttctccataa ccttcacctc aataccatta tacgaacata
      961 cattcccttt gtaaagaaga aactctgttt tatctttttg actttttgtc tattaccttt
     1021 cttaaaatca tattggcact tcctgcgaag atgaaggaca tgtactaaaa aaaagaggca
     1081 gcagtataga tttaagtagt tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat
     1141 gagtatcaaa ttgcaactga atttgatact cgttttttat tgttaaatca acgtttgttg
     1201 ttaatttaca ccacaaaatg taagttaaaa tgtatataaa aatgcgaacg tgaaataatt
     1261 attgtaggaa tgcaaattgg tgtgttaaaa tggttacaat ttactaagca aaaaggggtg
     1321 cattttccta tgcaatttct tgagccaaaa aataagaacg ctaagtctgt ggattgggaa
     1381 atatcagagc aagtaagggt gatagttaag caatacgctg aatatgcaga gcgtactgaa
     1441 agcgaagcag tggatgaatt cttattgaac attttagacg ataagaagtt tattgaatgg
     1501 attgccaaca agcgaagtaa taagcggatt gttgaaaaaa tgggtatcaa agatagagtg
     1561 ggttgataga caatgaccaa gcttaaaaga ttagccacga aggaagatga aattgtagat
     1621 gttaaaattc cgatttcaaa cgaagaatta aaagataggg ctaaacaata tgatttatta
     1681 actccaaaac gatttgcaac aaggtataac aaaatgttgt tcttgccaac gtcttttaaa
     1741 tggaacggtt ctgaatatcc cattcaatat aattactgca tcaatccgtt ttgttgtaat
     1801 tttgggaaag aacagcacaa attcaaagat gtaaaaggga aaccaagtcg atataaaatg
     1861 actggtagtt ccaaagataa aggtcataaa ggtatgtact gcaatgacaa tcctattggc
     1921 agaggtgtat cgcaaaactg tacagtaacc cctttatcta attggtctgt tgttgaagaa
     1981 attaaacgcc ttatagaaat caatagcatc caagatgttg agccagatta tcaatttcat
     2041 aaagaaggct gctcggaaga agaatcaacc ccattcaatg agccgaagca attttacaaa
     2101 agggggaaaa gtaggggcaa atctcaacgc tatcaatgta aggcgtgtaa gaagttcact
     2161 aatgttctac caaaaagaga agaaaccact acttatcacc aacaaaagaa caccattctt
     2221 ccaatgttcg ctaagatggt tgttggaagg gtttcagtca gtcgtacttg tgatatttta
     2281 ggaattggag ttggaaccta ctatcataag ctggagtggc tgtataggcg ttgtttggag
     2341 tttttagaac gatatgagac acaacccctt caaacaaaga aatttaacga gatgtggctt
     2401 aatacggaca agatgcacta ctacctaaat aacgtccgta agaaaggaca aggttcaaag
     2461 aaatatacgg ggtttgaaga ccttaatatg caaacgtata tagttgtatc tgctgaggtc
     2521 ttatcaagat atgtgttccg ttctgatgtt gcctatgatt ggaatatttc aatggatgaa
     2581 ttaaatgaag atacaagaaa attcaaagaa gaccatctca atacttttag tcgtaaaaat
     2641 gataggttag attggtctta ttatccacaa gaaccatccg ctaacgatag cgaaaatagg
     2701 aatgcttatc ttcacgaatt agggaaaatt actaatagga gtagatttgt cgatggttta
     2761 aatgttgatg ctccttatac tacaactgct cactattggt taattaaaca aatggttaat
     2821 gccgacgaat ggcgaatgat tagtgatgat gacttctcta ttagaaacgc cttttataga
     2881 gtattcacga aagaattaag gttgagcgat gcacatcatt ttatttgcca agttaacaag
     2941 acgaaaagca gaaaacaatg cttaaaagaa ttcggacaag caaaagcaga actgctcgat
     3001 tggggagata tcagaggatt caaaacaaag tttctacgga ctttggcatc tcattatcta
     3061 actgaattac taacaagtca tcaattccac gaagaggcta ttagtaaaga tggagaaaga
     3121 tatcgcaaat atgccgataa ccctatcaaa catccattag ccactaagga taaaggattt
     3181 tattcagttg attgtagaac ggatttatct gctctggaac caaacgaaat cgcaaagatg
     3241 ctattaaatg ttaatgacca ttcgaccaat agctttattc aacagataag acgttatata
     3301 tcatctcttg aaagaccatt aacaacagct cgtggtgata agaagagcta tatttacgct
     3361 aacttcaatc cgaagtatgc tcaatttgcg attacaattc taagaactta ctacaacttt
     3421 tgtcgccctt ttaaatcagc ggataaaaag gttttgacac ctgctcaaag attagggatt
     3481 acagataaac aatttgattg gaaagatata atttatttca aatgacaaaa agagcttgga
     3541 gtaatcccaa gctcttgtgc tttatcttta ctgttcttct tcaattaacg cacccgttaa
     3601 ctcaataagt tttttctttc atatacatga aaatagtagt tatacggatt tttatcattc
     3661 tttatccctt tttgggcaaa tacctcattc cattcctcat aattcacttc tggaaaaaaa
     3721 gtatctcctt cgaattcatg atgtattttt gtgatgtaca ttttctcaac ataagggaaa
     3781 aacaaattat aaatctgttc tcctccgaaa ataaaaattt cttcttcgtt tttacataac
     3841 tcaaaaacat cttctattga atgaacaatt tcacaaccat taaaggtaaa ccccttatct
     3901 ctcgtcagaa taatatttct tctgtcaggt aaggctcttc cgattgattc aaggttcttc
     3961 ctacctaata ttatcggatg tccctttgta gtatttttaa catattccca gtccttggga
     4021 atcctccaag gaatgtcatt ctctttgcca atcactctat tcttatccat cgcagcaatc
     4081 aaagaaactt tcatttataa cacagccttt ttaactaatt taccatctaa tttcttattt
     4141 ctatcaaaaa accttttctc attatcctgc ccgttagttg aa
//
LOCUS       DAZKOK010000165         4011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000165 DAZKOK010000000
VERSION     DAZKOK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.169"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..774
                     /locus_tag="ACG90W_002698"
     CDS             178..774
                     /locus_tag="ACG90W_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0478993.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="ACG90W_002699"
     CDS             898..2697
                     /locus_tag="ACG90W_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0478994.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="ACG90W_002700"
     CDS             complement(2975..3187)
                     /locus_tag="ACG90W_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478995.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3649..3798
                     /locus_tag="ACG90W_002701"
     CDS             3649..3798
                     /locus_tag="ACG90W_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478996.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1179 a          686 c          709 g         1437 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg agatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat t
//
LOCUS       DAZKOK010000166         3904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000166 DAZKOK010000000
VERSION     DAZKOK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.170"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            441..1934
                     /locus_tag="ACG90W_002702"
     CDS             441..1934
                     /locus_tag="ACG90W_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0478997.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2069..2365
                     /locus_tag="ACG90W_002703"
     CDS             2069..2365
                     /locus_tag="ACG90W_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0478998.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2466..3317
                     /locus_tag="ACG90W_002704"
     CDS             2466..3317
                     /locus_tag="ACG90W_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0478999.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3338..3550
                     /locus_tag="ACG90W_002705"
     CDS             3338..3550
                     /locus_tag="ACG90W_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479000.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1381 a          570 c          794 g         1159 t
ORIGIN      
        1 aaatatattt gtgcgttttg cgttgacaat ttagagtgaa aagaggtaaa cttaattcta
       61 tagaaaccac agataattca ctatttacaa agtagcaaaa gcagtggtac tctttaaaca
      121 gaaaaattac ataaaaaaaa gagacggaaa taattgattt tagcagaatc aattatttct
      181 gaatcacatg gttaggacat gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa
      241 ctacttatat taaaagttga tcggatacct tttccgattg cctttattct accaaaaaaa
      301 ggcgagcaac tcaacagaag aacgtttatt tagtgtactt aaattaaata ttcttaaaca
      361 ctgaagagag aacacatact atggctgaga ttaaagctaa actgtgttct ctttttgttt
      421 gagaaaggac gttacaatcg atgttgagcg aaatttattc agctattctt aaaaacggct
      481 tacgtacata caaatatcga aacagcacca tgaaacctgc aggtcatatt gattccagta
      541 ggagtggtgc tatctttgcg taccgtagca agcaattaat gaatgttggg cgtggtatgg
      601 tcattacttc ggaagaagct attttagaga acgaaaagaa actaacgcat tggacaccta
      661 atacctatcg ctttggtact tatgcggatg attatcgaac agtggttaaa ggtcattctg
      721 agaaaaactt aagtcaaatc aacacatttg tagttgatat cgattcaaaa gagaaccatc
      781 aaggcgaaat tattttagcc tgtttagatc aagtgggcta catgccaacg ctaattttag
      841 aatcagatca tggttatcaa gtttattttg tactaaagac accaggctat gtcacaaaga
      901 aaagcaattt caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt
      961 tggctgaaaa attgtccgga gtggatctag gatgtaacca cttcggaatt gctcgattcc
     1021 cacaagaaaa taatattgtt tttaaagaaa tgaactatca gtattctttt gctgactgga
     1081 ttcaatggtc gatgaagctt gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac
     1141 taattgtctt tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt
     1201 tacttcataa agcaaacatc gtcggcggag aaggacgtct tggtcgaaat aatgtgattt
     1261 tcacgctttc tttagcttat tactcttcag gttatgcatt ggatacttgc gaatacaata
     1321 tgttcacatt caacgaacgg ttacaggaac cgctatctga agcagaatta gtaaagattg
     1381 tacgtagtgc ttattctggt aattatcaag cagcaagcag agaattcatt attgaattgt
     1441 gccagcaatg ggtttcacca accttaaaaa ataatgatct gttcattcaa cgacgtggct
     1501 ggtggaaatt taaaaagcca cgagatcaaa gaaaatatag tcataaaaat gaatggaaag
     1561 aagatctatt ggcttatcta agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt
     1621 caaaaaaaga attggcttca caactaacga tgcccaagcg aacacttgat aagatcttaa
     1681 atgagttacg agaggagaat aaagtcttct acaaggtaac acgaggaaga aatggcggaa
     1741 tgatcattgc ttcagtcaaa acgttgttcg cgagggttat tcaactaagt aaagaaaaga
     1801 gactagatta ttcaacaagg atcacagagg tatttggact caacgagagc ttagtagaag
     1861 agacatttag acaattatca acccccttga accctcttaa agaagtgaat ctttttgaag
     1921 tagatacagg ttgaaaaata aaaggtgcaa attgccctat acaatatatc tatgatactt
     1981 ctgtaggtgt tgctcccttt gcctagctac gtgtaggtgg tatacttata actgcgtatg
     2041 cagtcttatg tatcatggag gaagttgtat gtcgttaaaa aattatgtct attcacatcc
     2101 tgttaatgtg tttatcattg gtcgtttggg attatctgtg gaagtgttct gtgagcttta
     2161 tggttttaag caggggactt tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt
     2221 gccgattgag tttcttcatg ggttgtcctt agcttctggg gagactatgg atcaggtata
     2281 tgattgtttg tgtgtgttag agcaggagta tattgagtat aaaatagcta acgagttaag
     2341 aaaaaggaag aagtatatcc agtagtagtt tttatttact taaaaaattg gttcattgag
     2401 caacctatcg ctgctaaagt agcaaaggaa aacgttaaag gaaagaattt agggaagaaa
     2461 tcgaaatgaa atcggacgac tggattgaac acacctggct tcctctgaaa agaaatagca
     2521 aacgaaagaa attttgggta ttgcaattat ggccattagg aaaactttgt agttacgggg
     2581 cacgacttat tcttgattta accgtttact cgattggagt atttggaacg gcttattact
     2641 tgataaatga tcccaatagg attgcagcat cagacatttt tacagtgcta atgacgttga
     2701 tttttgtcat gctattgtct atttttactc aattagtcat gcaatttacc aggcaaaaat
     2761 taacagcagg aaaacttttc aaaagcctat tcgatttaat tgttgtaaat gcaattctag
     2821 cgactttaat aaaacaaatt gaacaatctg gaagaacaac aaaaggagtg aaggattttt
     2881 tattgacgtt tattcacttt gtaaatggac acatgtattt gtttgaagtg ggtattattc
     2941 tcttttttgt cgggtatgta tccacgaaac ttattaaacg ttcaattgaa aaagaaattg
     3001 aaattgacct gattccgaca cttcttggtt atgagtacaa tgttgatttt ggaagatatg
     3061 acgaattgat caatacggat atcgttcaca cgagtatcca tgaatctact gttaagggca
     3121 tcagatctat tttaaaaaat ggccaggaaa tgaaagcaat agatcgaaga aaagagatct
     3181 acaattttta tgaagtcgaa gcaagcctac gatatggcat cggaaaattg tggttagatg
     3241 gaagtaatat tcgtcaaaac aaaccataca ttttttggaa cagtagtgat ttagaacttc
     3301 caatgaaaga taaataatta tggagaatcg tttgaaaatg attcctctat acaggattgt
     3361 gcttatagtt ggcatggagt taaaaacctt aaagaaggag cagttattca aagtgaaaga
     3421 acgtcataat cagttactat gtagtaaaac gggtacgatg aacattgaat accgaacaga
     3481 atatttagaa ttaacgaatc aaactgattt tttgttgatt cgaaagcaac aagagcaaga
     3541 aaaaaattaa tggctactta tagccaattg attgatgaat gggaacaatt caaggaatct
     3601 aaaattccag aacgtctcta aaagccttta tttgtaaaga gcataaggaa acgtgggaac
     3661 actttttaaa aaaagtgttc caataggctg tgcctattac gtttctcaaa aataaatgtg
     3721 ggaacaaaat atttaaaaat aggatagtga aaggatagtg aaggatagta aaaggagggt
     3781 agtaggatag caaaaaggat aggctccttt tcgctatcct ttttaaattg gaggaattaa
     3841 tatggaagta agaataagaa atgtggatga gaaaatcgta agagaaattg atcgaagatg
     3901 tgac
//
LOCUS       DAZKOK010000167         3882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000167 DAZKOK010000000
VERSION     DAZKOK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.171"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..546
                     /gene="nrdH"
                     /locus_tag="ACG90W_002706"
     CDS             322..546
                     /gene="nrdH"
                     /locus_tag="ACG90W_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0479001.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            705..2864
                     /gene="nrdE"
                     /locus_tag="ACG90W_002707"
     CDS             705..2864
                     /gene="nrdE"
                     /locus_tag="ACG90W_002707"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0479002.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2914..3879
                     /gene="nrdF"
                     /locus_tag="ACG90W_002708"
     CDS             2914..3879
                     /gene="nrdF"
                     /locus_tag="ACG90W_002708"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0479003.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
BASE COUNT         1319 a          702 c          717 g         1144 t
ORIGIN      
        1 taaatgagaa tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg
       61 tgatattatg agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca
      121 tagttttcca caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc
      181 agctcttttt tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag
      241 tgtttgttgt ttgacttttt tactaagagt tttatactat ctattataga taataagaat
      301 gtgttagagg aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa
      361 tgtaagatgg tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc
      421 gatgaacagc ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc
      481 attacttctg atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc
      541 agctaacatg actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca
      601 ccagtttttg tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt
      661 gcaatcaagc atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt
      721 tacaagatgt cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa
      781 tccctttgaa taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa
      841 atacgatgac ttttctatct attatggaaa aaatcaatta cttgatcgat caagaatata
      901 tagaagcaga attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt
      961 tggacgagca acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat
     1021 atgcgttgaa aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct
     1081 tcaatgcgct ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa
     1141 tgatccatca acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc
     1201 ggggcgaatt ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc
     1261 gctcaatcaa ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat
     1321 caaatcttcg tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg
     1381 ttccagtcat gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc
     1441 aaggagctgg cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag
     1501 caaaaaaaga aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg
     1561 tacctgataa attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat
     1621 atagtgtcga aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg
     1681 atcatctagt tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa
     1741 atgaaatctc aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag
     1801 cgaaccgcga gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt
     1861 tacaagttca aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacgg
     1921 atatctcatg taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg
     1981 gtcaatctgt gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg
     2041 atgtcgtacc accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca
     2101 tgggcttaca cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag
     2161 actttacaaa tatttacttc atgctgttga attattggac acttgttgaa agcaacaata
     2221 tcgctcgtga aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca
     2281 cttatttcga aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat
     2341 tgtttgaagg catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa
     2401 tgaaagatgg actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat
     2461 atatcaacga tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag
     2521 agaagaaaat cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt
     2581 attacacttc tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaatggcac
     2641 aacaacacgt cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag
     2701 gcatttatga atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac
     2761 attacgcttt ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg
     2821 cagaagaaat cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc
     2881 gcttattcat attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata
     2941 aactggaatg aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa
     3001 ttttggctag atacacgtat cccactttca aatgacctag atgattggcg tacattgtct
     3061 caattggaaa aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc
     3121 caatctgaaa gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc
     3181 gttttgaaca atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt
     3241 agtacactga atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct
     3301 tatctgcaaa aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa
     3361 aagaaaattg ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct
     3421 ctttattatt taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc
     3481 cgagatgaat ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag
     3541 ttacctgaag cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt
     3601 tacgaaaacg aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa
     3661 gtcaagacat tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca
     3721 ctctttccag atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc
     3781 acaagcaacc atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa
     3841 gcgatgaaag acgacgatta tttgattgga ttggaataag ga
//
LOCUS       DAZKOK010000168         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000168 DAZKOK010000000
VERSION     DAZKOK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.172"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG90W_002709"
     CDS             complement(245..466)
                     /locus_tag="ACG90W_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479004.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG90W_002710"
     CDS             complement(564..809)
                     /locus_tag="ACG90W_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479005.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG90W_002711"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG90W_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG90W_002712"
     CDS             complement(2033..3034)
                     /locus_tag="ACG90W_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0479006.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG90W_002713"
     CDS             complement(3193..3459)
                     /locus_tag="ACG90W_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0479007.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG90W_002714"
     CDS             complement(3449..3805)
                     /locus_tag="ACG90W_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0479008.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKOK010000169         3826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000169 DAZKOK010000000
VERSION     DAZKOK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.173"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG90W_002715"
     CDS             complement(573..1130)
                     /locus_tag="ACG90W_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479009.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG90W_002716"
     CDS             complement(1309..1920)
                     /locus_tag="ACG90W_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479010.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG90W_002717"
     CDS             complement(2033..2290)
                     /locus_tag="ACG90W_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479011.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG90W_002718"
     CDS             complement(2887..3165)
                     /locus_tag="ACG90W_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479012.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG90W_002719"
     CDS             complement(3199..3450)
                     /locus_tag="ACG90W_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479013.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1155 a          781 c          508 g         1382 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattt
//
LOCUS       DAZKOK010000170         3810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000170 DAZKOK010000000
VERSION     DAZKOK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.174"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      53..219
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            367..924
                     /locus_tag="ACG90W_002720"
                     /pseudo
     CDS             367..924
                     /locus_tag="ACG90W_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            982..3021
                     /locus_tag="ACG90W_002721"
     CDS             982..3021
                     /locus_tag="ACG90W_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0479014.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3130..>3810)
                     /locus_tag="ACG90W_002722"
     CDS             complement(3130..>3810)
                     /locus_tag="ACG90W_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0479015.1"
                     /translation="VLVLSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAP
                     FLLSFAEKVYANSSIIIVSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVY
                     HNVEALGYAVFIPVFFVSVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAG
                     FSQHSSLMVGAGMISRGEMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKY
                     FTKKVYEV"
BASE COUNT         1371 a          680 c          781 g          978 t
ORIGIN      
        1 cattgcctaa tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc
       61 ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca
      121 atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt
      181 acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga
      241 aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg
      301 ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt
      361 ggtggaggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
      421 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
      481 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
      541 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
      601 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
      661 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
      721 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
      781 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
      841 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
      901 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
      961 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     1021 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     1081 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     1141 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     1201 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     1261 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     1321 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     1381 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     1441 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     1501 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     1561 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     1621 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     1681 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     1741 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     1801 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     1861 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     1921 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     1981 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     2041 atagcttttg acagtctagg aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc
     2101 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     2161 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     2221 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     2281 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     2341 gacagttctt ggggatcttt taaaacaacg cgtgtcagta gtgatgtatc acaagtagac
     2401 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     2461 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     2521 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     2581 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     2641 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     2701 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     2761 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     2821 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     2881 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     2941 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     3001 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     3061 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     3121 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     3181 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     3241 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     3301 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     3361 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     3421 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     3481 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     3541 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     3601 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     3661 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     3721 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     3781 aaaagatagg ctgaaactta gaactaaaac
//
LOCUS       DAZKOK010000171         3809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000171 DAZKOK010000000
VERSION     DAZKOK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.175"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..362)
                     /locus_tag="ACG90W_002723"
     CDS             complement(72..362)
                     /locus_tag="ACG90W_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0479016.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(498..677)
                     /locus_tag="ACG90W_002724"
     CDS             complement(498..677)
                     /locus_tag="ACG90W_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479017.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(674..1024)
                     /locus_tag="ACG90W_002725"
     CDS             complement(674..1024)
                     /locus_tag="ACG90W_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479018.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1017..2342)
                     /locus_tag="ACG90W_002726"
     CDS             complement(1017..2342)
                     /locus_tag="ACG90W_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0479019.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2792..3406
                     /locus_tag="ACG90W_002727"
     CDS             2792..3406
                     /locus_tag="ACG90W_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0479020.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3571..>3809)
                     /locus_tag="ACG90W_002728"
     CDS             complement(3571..>3809)
                     /locus_tag="ACG90W_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479021.1"
                     /translation="FMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISI
                     TNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         1175 a          731 c          599 g         1304 t
ORIGIN      
        1 ttcttagaaa aaaacaatat tacgatatca tgatattaag atatcgtaat attatacaat
       61 tgtgattgat attatatatg tttttttatt tttttatcat gtttaatctt gaaggaatca
      121 tattcttttt ctaaagacag gagcttttca taaacatcag acatgtttaa actagaggcc
      181 aggcttaaat cataaacgaa tgaagcaggt aatgatttta ttctacgttg tcgggttatc
      241 cagcttgcaa ccgttccttg tgggtaggca tataactcac aaaattgttc tacagtgatt
      301 cctaaattct tgataatgta aacattgatc gggtgcgagt aaacaaatgt attgatagac
      361 atggtgcgta agcctacttt cttttattat tgcgtttgca ataatagttt aacacacgca
      421 ttattctagt atctactcct atttgtaaaa acaaaaaagg attcatagag ataactctat
      481 agaatccgta aaatacctta tatatataac gggaagatat aaaacctaaa attatgataa
      541 cctgctgcag cttctacata gaaagagaag cgctcaacac caacctcatt tatcaaacgt
      601 tggccataac cccaaatctc ttctgatagc tgatcaaagg ttatatactt tagctcatat
      661 tgctggataa tgttcattct aatcgactcc atttattatc ctggtaaaat tcgatatgac
      721 gtatttcgtc gtagtgaatt ttgtcttttc caacgtagat ccccagctca tcactaccta
      781 agatcttgcc aatgatatct tctggatagt atccttcagc gtttcgttct tctttttgaa
      841 tagatactga tctatttttt atgactgcag cgttcaatac ttccgcaatc tcttcagtcg
      901 tcatttgttc ttttgctaaa ttttgactgg ttcgttgttc attgtcggca tcaatttctt
      961 cagtatgatc agataaataa aaccctgccc actttaaacg tccacgatca ttgtaatcaa
     1021 tcatttttaa ttcccccatt tccgcccgca tgaccaccaa caagtgtgct tctagtaatg
     1081 cttctagcgc cttctaacat tgaagaagca tgtacaatcg acgtaaaacc atatttttgc
     1141 cgaatcttgt caataatttt atctaaattt tcttcgtcaa tttgcttttg tggatcacta
     1201 aataaatcca gctgaagcga atcggtgtac acaagcttgc tataggttat tccaacatgc
     1261 cttacttctt gtccttcata atacttatca aacaagaaaa gacaataatt ctgcaattcc
     1321 tttgtattat ctgtgatagg gatcttcatc tggtgtgaaa accctggtct cgtttctaag
     1381 atcgacgtgc caatgtgtaa atggacacaa cctgttttac agttatgctg cctaatgcgt
     1441 atcgctactt gttccgccat ttcttttacc accagctcga tttcatttct tctagtataa
     1501 tctcgtggta acacttgtga atttccatat gatttttcct ttgtcggttc tggtgggatt
     1561 gctatatctg ttcgatcgat cccattagca tggaagtaca gctgtaagcc aatcacacct
     1621 agtctattct taatcgtata aggattccag ttagcaagat ctctaatgct cataatcccc
     1681 atctgattga gtcttttttt cattcgagag ccaattcccc aaaagtccgt catttcagga
     1741 atattccata ctttttcagg aacgttttcg tacgtccatt ccgcaacaaa tccttcatta
     1801 tgctttgctt cgttgtctaa ggctagttta gccaaaaggg gattatctcc gacacctact
     1861 gtagcaatca atcctagctc ttctttaata ttctcctgga tcatttgagc cagcttcttt
     1921 ctccgttgac ttcgtgttcc ttcagtcgta aaaagattca gtgatcgggt cacttttaag
     1981 attgattcat cgatcgagta aatcagtaga tcttcatcag ccacatatct tctgaaaata
     2041 ttatttacct gcatatttcg cttgatatat aggttcatac gtggtggaac aacgtgtagt
     2101 gttttaggaa atggttgtgg taagtcacgt ggtctactca catttgtaat accataccgc
     2161 ttttttgctt caggagaaga agctaatatc aatcctgaac cagtattatc agctcgactc
     2221 ataacaacaa gttctgttgt taatggatct aaatttcttt ctatacattc gacacttgcg
     2281 taaaagcttt tcatatcgat taaaaagtaa tcatttactg gctcttttga ataatccatc
     2341 ataaataaca ctccattctt tcacattata caaacatatg ttcgatattg taaagaagaa
     2401 atagctaaat acataaaaaa agaagcatag gtatgctatc accctgcttc ttcttttgat
     2461 ttgtatgatc aatttattct atcactttac taattaattt gaaaatctaa taaaaactct
     2521 cactctaaaa gagtgagaga ctgcaaatta gagtacggac ttttttggag ttccgttctt
     2581 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
     2641 tacaaaaaat tctcatacta tttcctccat cattagtttt tgtctatata aggtttaccc
     2701 taaatagaca ctggtataac attgaaaaat atggcttcct cgtgtatgat tagggtgtat
     2761 tttaaatagt tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc
     2821 gatcgatcaa aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat
     2881 tttcatcgag aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt
     2941 agattacgtc cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa
     3001 ttatgacgag atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat
     3061 tactagttta ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa
     3121 ggatctgatc atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg
     3181 tcgccaggca caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa
     3241 attatacagt gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga
     3301 agatcttaaa aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca
     3361 aaccgtgtat agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct
     3421 tttattatct tctcatcttt aatttcctac atataattac tatctataaa attaagtctt
     3481 actcagaaca gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga
     3541 gtctgttaat ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt
     3601 atctatattt tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta
     3661 tgtcgacaca ttggttatac taattcctat agctagttct tgagactcta cttttctctc
     3721 tagctccttt gacaaagtga tttgagattt atttggttcc gttggagtca attctataga
     3781 ttcttcataa aaatctctat acataaagc
//
LOCUS       DAZKOK010000172         3664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000172 DAZKOK010000000
VERSION     DAZKOK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.176"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG90W_002729"
     CDS             <1..277
                     /locus_tag="ACG90W_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0479022.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACG90W_002730"
     CDS             420..641
                     /locus_tag="ACG90W_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479023.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACG90W_002731"
     CDS             657..1499
                     /locus_tag="ACG90W_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479024.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACG90W_002732"
     CDS             1562..3364
                     /locus_tag="ACG90W_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0479025.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ACG90W_002733"
     CDS             complement(3423..>3664)
                     /locus_tag="ACG90W_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0479026.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DAZKOK010000173         3602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000173 DAZKOK010000000
VERSION     DAZKOK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.177"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG90W_002734"
     CDS             <1..186
                     /locus_tag="ACG90W_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0479027.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG90W_002735"
     CDS             329..553
                     /locus_tag="ACG90W_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479028.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG90W_002736"
     CDS             568..1437
                     /locus_tag="ACG90W_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0479029.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG90W_002737"
     CDS             1418..2152
                     /locus_tag="ACG90W_002737"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0479030.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG90W_002738"
     CDS             2185..3093
                     /locus_tag="ACG90W_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0479031.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG90W_002739"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG90W_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAZKOK010000174         3601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000174 DAZKOK010000000
VERSION     DAZKOK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.178"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(218..895)
                     /gene="nth"
                     /locus_tag="ACG90W_002740"
     CDS             complement(218..895)
                     /gene="nth"
                     /locus_tag="ACG90W_002740"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0479032.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(902..1600)
                     /locus_tag="ACG90W_002741"
     CDS             complement(902..1600)
                     /locus_tag="ACG90W_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0479033.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1868..3052
                     /locus_tag="ACG90W_002742"
     CDS             1868..3052
                     /locus_tag="ACG90W_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0479034.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3058..3501)
                     /locus_tag="ACG90W_002743"
     CDS             complement(3058..3501)
                     /locus_tag="ACG90W_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0479035.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          931 a          720 c          604 g         1346 t
ORIGIN      
        1 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
       61 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
      121 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
      181 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
      241 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
      301 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
      361 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
      421 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
      481 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
      541 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
      601 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
      661 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
      721 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
      781 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
      841 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
      901 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
      961 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
     1021 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
     1081 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
     1141 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
     1201 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
     1261 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
     1321 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
     1381 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
     1441 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
     1501 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
     1561 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
     1621 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
     1681 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
     1741 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
     1801 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
     1861 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
     1921 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
     1981 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
     2041 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
     2101 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
     2161 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
     2221 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
     2281 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
     2341 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
     2401 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
     2461 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
     2521 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
     2581 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
     2641 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
     2701 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
     2761 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
     2821 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
     2881 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
     2941 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
     3001 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
     3061 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
     3121 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
     3181 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
     3241 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
     3301 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
     3361 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
     3421 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
     3481 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
     3541 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaagaaaa
     3601 a
//
LOCUS       DAZKOK010000175         3586 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000175 DAZKOK010000000
VERSION     DAZKOK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.179"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..402
                     /locus_tag="ACG90W_002744"
     CDS             166..402
                     /locus_tag="ACG90W_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0479036.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            421..636
                     /locus_tag="ACG90W_002745"
     CDS             421..636
                     /locus_tag="ACG90W_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0479037.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1339..2091)
                     /locus_tag="ACG90W_002746"
     CDS             complement(1339..2091)
                     /locus_tag="ACG90W_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0479038.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2106..3353)
                     /locus_tag="ACG90W_002747"
     CDS             complement(2106..3353)
                     /locus_tag="ACG90W_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0479039.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(3429..3575)
                     /locus_tag="ACG90W_002748"
     CDS             complement(3429..3575)
                     /locus_tag="ACG90W_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0479040.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         1221 a          564 c          533 g         1268 t
ORIGIN      
        1 aactttgaaa acagttcttg aacactaact ttttcattcc tgaatcaaat ttattttgga
       61 aattttacta ttcaagaata aacttgataa gtttcaggaa gatttttact gttaatcaaa
      121 tacgtatgtt acagtagcac cgtaaaaaat attatttaaa gaagcgtgtt taaacatgca
      181 aaatgtaaag gaactaagta ttgtggaaat gcacaaactg ttggtggagg caataatgct
      241 tggggaaaat tggggcaagt tgtaggtgga cttaccacag gggcagtagg tggtgcaggt
      301 ttaggaacag caatttgcgg accagcttgc ggtgttgttg gagggttata tggagctgtc
      361 gctggaggtg ctgcagctgg ctgggatgct cgaaaaaaat aaaaaaggag aataagtagt
      421 atgcatatta aaaacacaaa aacaacattt atattatctt cagaagagtt gaaaaatatt
      481 caaggtggga gtgccgtggg agttttaggc actacttttt ctggggcaac agcaggagta
      541 aaactttgtt cagccggagg accttatgca atagctgctt gtggtgtagg tggagcttta
      601 ttaggtgctg gattcagcat gtggaccggt gcgtaaacaa ttaaaaaata tcaaacctca
      661 agattggttt gtgtcgatta cgaacatagg tatgttcttt atcttttcaa aagtttggaa
      721 tggacagaac tttttactaa agcttcttgt gctagttttt ataattatat taaattttta
      781 tcttttatta aagtcagata aaaattcttt ggaaatgtaa tggtagagat atttttacaa
      841 aaggacttgc agcgctgtag ttggtgctgc aggaggacct atttcggccg ttacaggagg
      901 tattggtaat gtacttgtcg gatgtgcatt aaagcctgtt cctgcaagtt aaatttataa
      961 aaacaggaaa gtaattatct caaatcctat caagtacaat cataggattg cacttgacag
     1021 gacaaatttt ttaactaaaa aatgaaaaac actttctaaa aattagtaga atttgccaaa
     1081 tctttcgcat cttccaccat acatttaatt ttaattcccc tttatggtac ttaataaaaa
     1141 caagaaaaga actattcatt tggccctttt ttggttccag accatagaaa tattcctcca
     1201 gtaactaata ttgctgttat gcacgtaact aataacagct ataaaaattg gtacaacttt
     1261 aataggattc agaaaaattt gcactagtga aggaatctat tttattagaa aaatgcaatc
     1321 tccctaaaga aaaaaccact aaacagtgct ttctttcaac ttctccttaa ttttttgtgc
     1381 tttccctaaa gtaaagtttc tggccaactc tgaatcaaaa tatagtgaac gttttttaaa
     1441 atctatttct ttaacgttaa agatatttac taaacacgct cgactaattc ttgttaacat
     1501 aggatatttc ttttcaagct cactaattct tccataaaat tcgtactgcc catctttagt
     1561 acacaatgat aatctatgtg gtaattttga agcttctaca aaataaatgt catttatatc
     1621 aaaagtaaat gtttgagaac ctatggaaaa aacaaatgct tgattcgtaa cctgttttgt
     1681 tgcatctatc ctttcttgcg ctaataacaa taactcaaca atctcgtttc gatactcatc
     1741 tagtgtttga tcttttgtta caaatcctaa cgtttcaact cttcttttta ttgttacggg
     1801 taacatctca tcatgagtag ttgtaaaaat gatttttgct tgaacatcat attttctgat
     1861 tacttctgct aattctatac cgttaacttc atgatttaaa tcaatatcta agaaatatat
     1921 tccttgcttt ggtcggaatt gtttgaggta ttttttaact tctaacggac tttgtgtttt
     1981 caatactatc ttaaatactt tatcgtgaaa aagaatgaaa ttatcaataa ttcgttctaa
     2041 ctgatttaat tgtataattt gatcttcaca taatattatt ggataactca taaatcctcc
     2101 ttccctcaaa acaacaaaat aacttgagta gtaaattgat acggatcgtt ttgatagttc
     2161 acaaacatat tgggattctt tttattgatt tcctctatac tagataaacc taacccttga
     2221 tgtccctttt ttgttgttat tccttttttc attagagtat ctatagagag tagtatctct
     2281 ctacaactat tcgatatcga aatttctatc tgtttatcat ctcggtaaat agctagagac
     2341 agttctctat cttgactctc tatagatgct tcaattgcat tatcgagaat aattccaagc
     2401 accctaatac agtcaaatat tggaacagga acttcaataa tagtacttgg acattcgaat
     2461 ttacaacaaa tcttattgtt atttgcttgg tatagcttcg ctatcaacaa acttttcaaa
     2521 tattcatttt ttatattttt taaataacga tattcaaact tattggttag ataagaatta
     2581 gaatagatct ctaattcttt tatttctttt tccaatatcg aatcatggtt ttgattagcg
     2641 atttctttta aactcagcaa taaattttta tagtcatgtc taaatctggc aaatttttct
     2701 tgatcttttt ccagtgaatc agtatatttt tttagataaa ccaattcttt tcttttcaat
     2761 tgctgttcat attgttctcg ttgcctaata gttattctaa taaataaaaa tagaacaaca
     2821 aacatttgaa tgattaaaaa tattaagaca ccaaacacaa aatgatcaaa tacttgatat
     2881 tgatgagcaa cataagaaac aaataaacca attgtaagta aataaatcat gaatatagca
     2941 gtaagtttag aactggactg ttcaacaatc ctatttatag ctaattttct atataaccaa
     3001 ataaacatca gcaaaaacaa agaatcaagc attatctgga atactacata accataccct
     3061 tttatccctt caaatgaaaa catatacgtc ataaccatgg aagtaataat agatctgaaa
     3121 tcatcaataa gcatacataa tagtaattta tttaaaagca ctatattagt tttcttcttt
     3181 tcctttaata taaaataact tccgattata ggtatagctg cggaaacatc agtaaataat
     3241 tcaacaaaaa aagtgagtgg tatcaataaa catacaaaaa gatattttct tttgttaaga
     3301 tcagtattag tcatataaaa taagagaaaa aagaaaaagt ttcccacgaa cattcttaat
     3361 agataagctt ttgtatcgat agtaatcaag agatcccctc ctgattttag gatatcataa
     3421 aaaaaatttt agttacactt tttaaataat gaaaaaacac attccactaa atttgatgcc
     3481 ggttttcctt gaggttttgt tccagcacct cctttaattt ttttaagctc ttgatctgaa
     3541 cattgtttag catttagtct atttttttct tccattacga ttcccc
//
LOCUS       DAZKOK010000176         3531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000176 DAZKOK010000000
VERSION     DAZKOK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.181"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(257..961)
                     /locus_tag="ACG90W_002749"
     CDS             complement(257..961)
                     /locus_tag="ACG90W_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479041.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1148..1393)
                     /locus_tag="ACG90W_002750"
     CDS             complement(1148..1393)
                     /locus_tag="ACG90W_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479042.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1410..2261)
                     /locus_tag="ACG90W_002751"
     CDS             complement(1410..2261)
                     /locus_tag="ACG90W_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0479043.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2427..3431
                     /locus_tag="ACG90W_002752"
     CDS             2427..3431
                     /locus_tag="ACG90W_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0479044.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          601 c          687 g         1145 t
ORIGIN      
        1 tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa
       61 tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata
      121 ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt
      181 tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt
      241 atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag
      301 gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat
      361 tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa
      421 taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg
      481 aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt
      541 ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg
      601 tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca
      661 cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa
      721 ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat
      781 ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt
      841 gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc
      901 ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca
      961 tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag
     1021 gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc
     1081 cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc
     1141 attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt
     1201 catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga
     1261 tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca
     1321 aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt
     1381 cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct
     1441 ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa
     1501 tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg
     1561 aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca
     1621 gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct
     1681 tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt
     1741 gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga
     1801 tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct
     1861 tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg
     1921 tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct
     1981 tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag
     2041 aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc
     2101 ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag
     2161 gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg
     2221 taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta
     2281 ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc
     2341 ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt
     2401 aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata
     2461 ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag
     2521 aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa
     2581 ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt
     2641 ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag
     2701 aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat
     2761 ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag
     2821 ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag
     2881 atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg
     2941 agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac
     3001 cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat
     3061 ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag
     3121 aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca
     3181 cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc
     3241 ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat
     3301 caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat
     3361 ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga
     3421 atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa
     3481 ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt g
//
LOCUS       DAZKOK010000177         3440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000177 DAZKOK010000000
VERSION     DAZKOK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.182"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..385
                     /locus_tag="ACG90W_002753"
     CDS             68..385
                     /locus_tag="ACG90W_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0479045.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            382..543
                     /locus_tag="ACG90W_002754"
     CDS             382..543
                     /locus_tag="ACG90W_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0479046.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            546..845
                     /locus_tag="ACG90W_002755"
     CDS             546..845
                     /locus_tag="ACG90W_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479047.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            845..1159
                     /locus_tag="ACG90W_002756"
     CDS             845..1159
                     /locus_tag="ACG90W_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0479048.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1152..1682
                     /locus_tag="ACG90W_002757"
     CDS             1152..1682
                     /locus_tag="ACG90W_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0479049.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1679..1948
                     /locus_tag="ACG90W_002758"
     CDS             1679..1948
                     /locus_tag="ACG90W_002758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479050.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1878..2081
                     /locus_tag="ACG90W_002759"
     CDS             1878..2081
                     /locus_tag="ACG90W_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0479051.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            2078..2365
                     /locus_tag="ACG90W_002760"
     CDS             2078..2365
                     /locus_tag="ACG90W_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479052.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(2398..2649)
                     /locus_tag="ACG90W_002761"
     CDS             complement(2398..2649)
                     /locus_tag="ACG90W_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479053.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            2852..3265
                     /locus_tag="ACG90W_002762"
     CDS             2852..3265
                     /locus_tag="ACG90W_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0479054.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         1276 a          513 c          774 g          877 t
ORIGIN      
        1 ccaaactctc caccaaaatc ttcctaaatg ctttgatacc aacgattaaa cagtctggag
       61 ggttacattg aaatcagaac atcaaataca agacgagatc agaattgcct tatcaaaaca
      121 tggttgcacg atttttcgat ccaatgcggg acaagtgaca acgatcgaag ggcggcaatt
      181 ttacgggatg ccaaaagggt tcccagatct atgtggacat cgtaattctg atgggaaagc
      241 aatttatata gaagtaaaaa atgaacgcgg taagttgcgt gaagatcaaa aaaggtttgc
      301 tgaattttta aacggtcaac ctgtactata cggtgttgca cgatcagtgg aagaagcatt
      361 gaaaattgtg gaggaatcat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc
      421 cttcagcgtg agattgacga tctggaacgg acaaaggcag tgatggtcaa tgaaacggcg
      481 aggaaggcaa tcgatttgca catagagaat ttaagaaggg aaatccatcg attggaggaa
      541 tgagcgtgga taagaaagca gcaatgaaac gaatcatcga actgacacat tctgagaatt
      601 ggcaagaaga caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggagtg
      661 aaaaaaccaa acggagaaca ccgagaaaaa ttgcaatctg gcatggtgac cgaattctag
      721 taacaggtac tgctgaacag ttatctgaaa ttactggatt aagcaaaaac attatctggg
      781 atagagctaa acgcgagaac gttgattcga aaggtcgtca atttaaacat tgggagaaga
      841 aataatggag gaactaatca caaaagtaga gcagtgggca aaagataagg gattggatca
      901 agctgattcc agcaagcaaa tgttaaaaac gatcgaagag attggggaag ttgccgcttc
      961 tctagctaga aaagatgaat atggtttaag agacggaatt ggagacgtag tagtaacctt
     1021 gattatttta gctatgcaaa atgatatgga tttgtacgag tgtctgaacc aagcatacaa
     1081 cgaaatcaaa ggacgcacag gaaaaatggt agatggtgta ttcgtgaagt cgagtgattt
     1141 ggaggacagc gatgaataaa aaagtattaa ttgataaaca agcattgatt gatgaattaa
     1201 tgaaaatacc tggtgtggga tctaatagtg acgctttaga aacgattaaa cgtttcccat
     1261 cgtatgaacc gcagaagcca gttgtgccta agtttgtggc ggaactactc gactattatc
     1321 gtcagtcaac agatgttgat ttattagctt tattgataac tttccatgat tggtattatc
     1381 gcaaaactaa agacggtgag catgaagaag caattgattg gctagttgat catcctgaaa
     1441 tttatatgcg tgcatggctg tttggctacg agctcgagaa agagccgttg tattgcgtaa
     1501 taatcaatgg tagatatctt gtcaaagtat tcagcaatac aaacgttgtc atattagttc
     1561 cagcagatga attttcacag tacgttactc aaaaatttca attaactgaa aaacaaatca
     1621 aagcaattga tgaaagatac tggccgtttg ctgtgccagt ggatgaggtg gtagaaggat
     1681 gagcgaccca tacattacta aattagaaaa gttgtccgaa gattttgaga agaataaaaa
     1741 taaactaact aaagataaac ttatagaact ggttaggttg gttatcagag gagctattac
     1801 aagagaaaag gcgcagcaaa acatagaaat ttttgctcgt agtgtccgag atggattaga
     1861 gaagaaagtt aaacaaaatg aacaacagac accgcagaat aacaaaacta agaaaacggg
     1921 aactgaatgt actaaagaca aagtttgaaa aagaatatgg aatttcagca gaagaaacat
     1981 ataaagtggc aagtcagtgt gttgctgatg cgagcggcgc tattcgtaag tttggaattt
     2041 cgatattaag tgatgatcgt aaatgggagg aaaaagaatg aaactaaaag acggatttta
     2101 ctccagcagt catggtatcg gcggtttaat gctagatatg ccgacaaaga accctaaaac
     2161 acgtgagaaa ccaaaattca aagtcggtga catggttcgc tgtgaagcag aaggattcat
     2221 ctatccattt tgtggatatg tagaacatct ctataatcac tcagcaatca ttcgtattga
     2281 aaacacgatg gaatgtgaca agtggttagc gaaaagcaaa gagaatttag cagtagcgag
     2341 attggtggat atggaggtta tataaacagc gaaaaaatgt acttctaaga gaaaaattta
     2401 ttttttataa aaataggaaa gtatcattat gaaaaagacc acagagataa ataaacctac
     2461 cacataaaaa atgaatattt cttgcgattc aatataggta tatttaacaa tttgtacaat
     2521 aaaaaatact ataggtaaaa aagataacat gaggagctta tttgttctag agtgtttgac
     2581 aaagtaacaa aatgcgacga taccaataca aacgggaatc caaaataaca gcatgctcca
     2641 catagtcact caaccatctc ctatacgctt tattatcaat agataatatc atttttcgta
     2701 ataaagataa aaaaataagt aaggcttctc attaaaatat aaaaaagaca gccgaccact
     2761 gactgcctat ataagagtat tgaaataaaa agctgctgat ataataaatt ccacaagttt
     2821 attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg tagatttcaa
     2881 acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg agcggatggc
     2941 aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga aggcgccgaa
     3001 gcacggcaat aaggcagaag acgcaatcat tcagatgatg gatatagaag cggagagaga
     3061 cgcgattcta gcagccttga tggctcttag tctgattagt cgtcagatac tctactatag
     3121 cttttgtgtg ccagatagct tctcaaacta cagaattagc cgtgaagtgg gttattcaga
     3181 aagaagtata caacggatga agtcggaagc tctaatagag tttgcagaag catataaaca
     3241 cggaagaata attgcttata aataatttgg cggctttttg gcggaatgat ggcggttttt
     3301 agctatttac cagtgatatt atggtagtgt cgaaagatta gtgataggtc taagacaaaa
     3361 taataataaa aggaacatcg ttttattatt gtttcacaat taagcttcga tagacagcaa
     3421 cggaaatatt aagaataagg
//
LOCUS       DAZKOK010000178         3424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000178 DAZKOK010000000
VERSION     DAZKOK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.183"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG90W_002763"
     CDS             complement(31..1203)
                     /locus_tag="ACG90W_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0479055.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            2039..2371
                     /gene="traE"
                     /locus_tag="ACG90W_002764"
     CDS             2039..2371
                     /gene="traE"
                     /locus_tag="ACG90W_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0479056.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2990..3331
                     /locus_tag="ACG90W_002765"
     CDS             2990..3331
                     /locus_tag="ACG90W_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0479057.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1102 a          549 c          685 g         1088 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttatcttt tacacaatta tatggacttt atcaaataat agctatttta
     1321 atagaattta taagttaaaa tattatgata aaaatcacaa acgtagcctt ttacgtcata
     1381 agtgttaaaa gaaataatta atttcaaata tagaaaaggg aacgtatgtt tgcatactcc
     1441 ttttttttga gttataataa tacgcgaaga gagataattt cattatttaa acaagctaaa
     1501 aatcttgata ttacttcttt tcaggcaata cgaatattat tcgcttacat tttttgtata
     1561 aatttaattg tctgctatgc ttgtgatgaa ttgattttcg ttttgcactt ttggatgata
     1621 aatttggaca attgaaaata caaaaaaaag aaaggcggta tgaactcgga aggctggtaa
     1681 ccttcgtcag cgttcataca cggcattaag atttggccct taatgcgtta acctttcagt
     1741 gttacggtat tacccgcaat ggatttagta taacagatat tcaaaagtga taccactttt
     1801 tttggctgtg ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcataat
     1861 gttataaaaa tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt agagggaacg
     1921 catgtttgga tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat tctctatttt
     1981 tgaaaaataa caagaaaaac ttgttaaaaa tatttaaaga aattgggtgg tttattttat
     2041 gtcatataca ggaattttga gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc
     2101 tacagagaaa atcacgaaga aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa
     2161 gaaatatata atccagaaag acaaagtctc agaggaaatg atttattaca ttgggaaaca
     2221 aaagcaaata attttaaaag atcctatccc cttaaaagag ctatatccaa caatcaaaca
     2281 tgtttatgac caaaacggtg tactgattgg tcgaagaaaa aatggagtct tgcgttgtac
     2341 cgcaaaaggg atggggcgtt tgatcggctg aattgatcat tctcataact tgtattttat
     2401 cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa ggaacttaga gtcactagaa
     2461 aaataaggag tagacttgat ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt
     2521 aacgattggt aacgtaaaaa attggtcaat cgagtaaatg cgcttgtttg gatagtggtg
     2581 aaatgaattt gtgtaaataa taagcgcaga gtaaacaaga gggaatttag aagttagaca
     2641 agttagaata gcgtctagat taatcatgag cagctactaa gggatctaga ctttgacgca
     2701 aactcatgga ataaggagta ggtcttctcg taatggggag gacgcacaag aaattgtgtc
     2761 gtgtgctgca gtacatgtag atacttgcga ggacgttgat tattggatca atcgagcatt
     2821 taccgctgtc tactagggtt tctcgtttcg gacgccataa acgagtcgaa attttttatg
     2881 tatttttaag taaacgaaaa acgataaaat atgaacaagt cctgaataaa gtgaaaaaat
     2941 taatataaag catagagaaa atgaaattta cgtaatggag gaatgacaaa tgaaaaatta
     3001 tgatccgaat atacgattgg gcacacatac tattaaggtt tctttccaac gttgggatta
     3061 caaaggattt gtgacgttta gaaggggcgg aaattgtaaa ggattagatg ttttagcatt
     3121 agacgaggat gatttatacg atcaaacatt gactgataat ccaattggat tcggcttgct
     3181 acctgaagat gatgaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga
     3241 cgaattatct gttgaagata cttggtcata tttgagtgat tatattgtca gctttgaaat
     3301 cattgaattt gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa
     3361 ggcttagaag gagtcacgac gattaataaa aaggtagtat taccttaggc attgagtaag
     3421 gaat
//
LOCUS       DAZKOK010000179         3263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000179 DAZKOK010000000
VERSION     DAZKOK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.185"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..699
                     /locus_tag="ACG90W_002766"
     CDS             <1..699
                     /locus_tag="ACG90W_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0479058.1"
                     /translation="FKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNS
                     AENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIK
                     GIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPS
                     VQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQM
                     KRDAGAVGGTLYAT"
     gene            689..1429
                     /locus_tag="ACG90W_002767"
     CDS             689..1429
                     /locus_tag="ACG90W_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0479059.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1426..>3263
                     /locus_tag="ACG90W_002768"
     CDS             1426..>3263
                     /locus_tag="ACG90W_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0479060.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1226 a          397 c          632 g         1008 t
ORIGIN      
        1 ttcaaagata cttggaaaaa tattaaagat acggcataca atacgtggat ttctataaaa
       61 aataccatga ttaatacatg gaataacatt aaggactctt tctggaatat tgttactgga
      121 attgttaatt ctgctgaaaa tgcatggaca aatctaaaaa atggtgtttc aaatgctatt
      181 aatcgggtaa aagaaatctt taattcgtta agggaagtca atttatttga aattggcaag
      241 aacatcattg atggacttat aaatggtgta aaagaaaagt ggaatgcttt gaaagatact
      301 atcaaaggta ttgcaggtaa tatcaaaaat tggattaaag gagctttaga tattcattct
      361 ccatcaagat ggatgcgtga tatggttggt aaaaatattg ttcaaggtat tatcgttggt
      421 attgataaag agcaaagtaa gttagatcaa accatgacaa atttagtaaa aacaccgtct
      481 gttcaacctg tagtaaatgg aataagtcac ctaccaatta cacaaacaaa gcaaaatata
      541 tctgctagtg aaacaaaaga aattcattta catttaaatg tttatggaga tttacctgat
      601 tcaatggttc ggaagattac cacaaaaatt aaaacagaat tgacaagaca aatgaaacga
      661 gatgctggtg cagtaggagg aacattatat gcaacttaag agaggacaat tttttattaa
      721 tcaacattat tcttctgaat ttaatgtgta tattcaaaac aggcctgctt ctgtttcagc
      781 tagtcgagta attgaattga gagagcgaga aggaaatgat tctatcatta ttgataaagg
      841 ctattacaaa aatgtgacta gaaagattga gtgttactat aaagcacctt caattgatat
      901 ggtgcaggag tgggaagatc gaatcactga atggttagat atgtcgtctt atagtgattt
      961 tattctttac tatgatgaac aatatattta tcaagctgta gtaatagaag gacctgaatt
     1021 taaaggaaca agaaaaacag gaaatattgt cccttttgaa tttacagtga gtattcgacc
     1081 atttaaggaa aattacaatg gtagatttac tattcgacaa acagaaactt ttgaaattta
     1141 taacccagag aagtattctt caaaaccgct tattaaattg agtggttctg gagatgcttc
     1201 tttttatatt aataaggata aatatgattt gaaatcgttg gacagagaat tatttataga
     1261 ttctaaatta gaagaggctt atcgaaaatt agatggtaat ttagaacatc aggatcaggt
     1321 caccttattt ttagattttc catttttata tcctggaaaa aatgaaatta aatggacgaa
     1381 aaatattcat tcatttgaaa taatgcctag gtggtggaga aaagtatgaa accaagaata
     1441 tatagtccta ctgaaacaga tttctcaacg aatggcttag gaattttaaa ggattgtaca
     1501 agatgtggaa tatatgaagt agctaatgga aaatatgaat tagagttgga ttatccttta
     1561 ggaactagat ttgatgaata ttttgaaaat gactatcaaa taaaagcaaa gccaaacgat
     1621 caagaagagt atcatatctt ttttattgat gataaagata tagatacttt tttaaataca
     1681 gtaactattt atgctcagag tcgtacaaat cgacttggaa gacgggtagt cactcttgtg
     1741 gaagtggact ctaaaactgg tcaagaagcg atatcaatta ttgaaactaa aatggataaa
     1801 aaatctgaca tacgacttta ttctgatatt acggctgttt ctagcacaat ctttgaagcg
     1861 agaaatgttt taaattgtat tgctggtgaa caaggatcat tgcttcaata ttggggcgga
     1921 gaaattaaac gtgaaccatt taagctttct ttgttaaagc gaagaggtcg caataatgtt
     1981 ggaacgattc ggtatggaaa agatatgtct ggtttgaagg tcaaattaga ttggacaggt
     2041 ataaaaacaa gaattattcc atatgctgat cctcaaagtg aagtaggtac gactaatcga
     2101 atttatggtt cgccagtaga tagtgcctat attaataact atcctgatgt ctatacagaa
     2161 catgttcagt ttacagaaga acaaggagta aaagacatta agagcttaaa taaaatagct
     2221 aaaaattact ttaaaacgat caatcctggc tgtgataagc ctaaagtttc tattacggtt
     2281 gaatttgata agttgactga tagtgaagaa gcgaaagaat ttgcgaagat tagaaattat
     2341 ggtctgtttg atacatttaa aatatatcat aaaaaatata atatttatct tgaatcaaag
     2401 gttagtggac tacaatacga ttctttatct gaaaaaactt tgaaattaga agcaggagac
     2461 attcaagttg ctttttatca gcaacaagct gtaactattc aggataaatt aaaagattat
     2521 gcaacgaata attatatgag tgattttaat gattatgttt cttcaatgat tacaggtcaa
     2581 ggcagtgcag gaggatatgt agttttatgg ccaaaagaaa aaccttccaa catctttatt
     2641 atggataatc cagatttaga aaaggcaaga gaagtactaa gaatgaataa aaatgggatt
     2701 gctttttcta agaaaggatg gcgggggcct tttaattcag cttggacatt agatagtata
     2761 tttaatgcca attttataca aactggatta atcaaagcag atatctttca aaattctttt
     2821 aacaagactg gagatgtatt gaaattagtt aatggactac tccaaatttg gaacaacaaa
     2881 aagaaaataa tggagttaac taaaaaagga atggaatttt ggaattctaa gggttcaatt
     2941 ggaacgattg gaacaactga ttctgttggt aatccttttc ctggggcttc tactccaacc
     3001 cctattgaag ataattcttt agttattcgt acaaatggag acggcaaata tattttgatt
     3061 tctcctaaag taggaaaagg attagtttta ttaggaaatg gtaaagcaat ttattttgga
     3121 gacttagatg tacaaggaaa acttacagtt aaaggaaaag aaataattcc tggacaaggt
     3181 ggtggtccat cgggaggtgg agaatctact ggtgggtatc ctagtgaatt aaaaacagat
     3241 gcagaaaaga gagcttggag aat
//
LOCUS       DAZKOK010000180         3258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000180 DAZKOK010000000
VERSION     DAZKOK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.186"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..1032)
                     /locus_tag="ACG90W_002769"
     CDS             complement(85..1032)
                     /locus_tag="ACG90W_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0479061.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
     gene            complement(1037..1789)
                     /locus_tag="ACG90W_002770"
     CDS             complement(1037..1789)
                     /locus_tag="ACG90W_002770"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0479062.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            complement(1790..2698)
                     /locus_tag="ACG90W_002771"
     CDS             complement(1790..2698)
                     /locus_tag="ACG90W_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFQ0479063.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(2698..>3258)
                     /locus_tag="ACG90W_002772"
     CDS             complement(2698..>3258)
                     /locus_tag="ACG90W_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0479064.1"
                     /translation="YQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIID
                     TFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGE
                     KILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLL
                     EKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT         1071 a          612 c          405 g         1170 t
ORIGIN      
        1 tatcccagat ttgattttac aaaactcatc agtccttatt gacaaagagc ctgaatttac
       61 cctctaatct tatcttagta tgttttaatt gatttcagtt ttcaaataat tagtcattcc
      121 acttaaaata ccatatttcg gttgatagcc aattgactgg atcttagtaa tatctgctag
      181 agagtcatgg atatcacccg aacgctcttc tttgtattca agtgtcaatt ctacaccgag
      241 aatttcattg attgcatgaa tcaattccaa aagtgtggtt gctttacccg ttccaacatt
      301 gaattgtttc cccagtgcag cttcctcatt cgcaactaaa agtaatgctt ggattacatc
      361 atctatataa acaaagtctc tagactggct tccatcacca aataatgtaa atgaagttgc
      421 ttctcctacg atttgtttct tatagcgatc tacgagaata gaaatcacac cactgtatgg
      481 agaattcgga ttttgatttg gaccatagac attgaaaaaa cgaactgcgc taccgggtac
      541 atcatataaa tgacaataat ctagcacata ttgttctgca gcgaatttat caaccgcata
      601 aggcgtcaat gggcgaatca cggactcttc tttttttggt aaagtcggtt catccccata
      661 aacagccgct gaagaactaa acacgatccg ttttaaatca ggttgatact tgcggatcaa
      721 ttctaaaatc attaatacac tttcaaaatt cacacgatgt gtttctactg gtcgggcaac
      781 tgaatctgcc acactagcaa ctgctgctaa atgaaagata taatcaaatt tattagcttt
      841 catgatctct tccatcaagg atttctcagc gacatctccc tcaataaaag tgatattttt
      901 cgtcatatca agattttcta cttttcccat tgataagtca tcgacgacga ttaccacatg
      961 ttcttctcct aaatgattgg ctaaggtaga accaatgaag ccagccccac cggtgataag
     1021 ataacagctc atccttttac ctccgatata aataattata gatatcttga agctccgaag
     1081 aatcttctaa aaatttagga aacaaaaatt ctaaccattt aaaatcttct tcactatcaa
     1141 tatctaaaat tagataatct ctcattttta ttatcccaca ttgcccatca aaaatataat
     1201 tacttttttt tagaaattca ggtttaaaag aatacattga ggcattcatt tcaaaaactt
     1261 caggtgtctg ttgcctagat gtgtatcttg atttattaac tatagtaact tcattttcaa
     1321 ttctttttac catattaaaa tagggattac gtcttgcaga aacaactgaa aagtagacgt
     1381 ctttatctaa attttttaga tgttcatcta ttaattcttc aagattagaa acttcacgta
     1441 aaggtgaagt caaatctaaa tctataatta aatcaaatat attatcttct ctttctagtt
     1501 gtaagaatga atcacgtatc acttcaattt tccctacagt atctgtagct aattcaggtt
     1561 tacgttctac aattttcaca gacggattta acttagtcgt caattctact aactcattac
     1621 tgtccgtatt caaacaagtc gtgcaattat gttcaggatg tttttgttga tagatatcta
     1681 tcattgctaa agcgaaatgg actaggggct ttcctgccaa atatttcaaa tttttatttt
     1741 taaacccctt agacccagct ctaccacaaa tagtaaataa aatttccatt tatttctcct
     1801 cagctatttt caatacttcg tttgcatgat ataaatcatt ccagttttct ttgcctgtta
     1861 ctacgttttc aaagaaatat ttcaactcct tctcatacat atcattagca gattcaggca
     1921 tttctttaac aatgttttct tcaaaactta ttttattatt taataagtca acattgatac
     1981 attcatctaa agtaaacaat tgaaattttc ttgtaggaac tcttccaaaa taatctaaat
     2041 gaatttctat aattttatca tcgtatctca atatatatgt tgctaaatca ttactgttaa
     2101 tttcaagatt cgaatattta ccaaactgtt tttttactga tttagggaaa ccaaataggt
     2161 ttataatata atccaattca tgtatacagt ctaattctac tcctcccccc atagatttaa
     2221 aggcagaata attatttcta taatcagtac cttttctcca atcaggaaga taagtagaac
     2281 aaatagctct tacagaatat acttttttac tttgaatact attacgaaca acttccatga
     2341 tatttttata cctcagtgga gcagcaacat aaatattttt gtaatcataa ttatattgag
     2401 taagatcaat actctcagaa aaagcaggtt tttctaaaaa tatgtaatca actttttcaa
     2461 caattacatt tagtgtttcc agatgcttaa aagtcggatt tgtaataaaa gctatatcat
     2521 aatgtgtatc caaatcgtct ctattttgaa tatacgttat ttcttcaaac tctttttttc
     2581 cactgctact ataagctgtt atatcaaaat taatagaatt cttttttcca tagcttatta
     2641 gattttttag atgtctcttt ccaattgacc ctaacccaaa aaaaattact ttcatcatta
     2701 atttacctca atgtcgttaa ttaagttaat aatatactta tctttttcaa aagtatattg
     2761 gggttccatt ttcttttcca aaagcgtgaa aaattcttta atctcctcta aatatgcatc
     2821 ttcaataata gtatgtgaat aattattatc atgcataacc tcatcgtaca attgaatatc
     2881 tgtcatacta gaagtttctt cattccatga aaatagagaa tccgggttac cttcccattc
     2941 tattaaaatc ttttcaccaa ttatagttaa atttctttta gctactcttg aaacaacatc
     3001 tacacttaaa gttccaacta taccagtttc atgctcaata ataagagtga aactgtccga
     3061 ataatcaata tcaagattgg ataatttctg ggtcattaca gtaaacgatt taactttccc
     3121 aaatgtatct ataatccaag gtaattcaat agcgaatatt tctctacacc cattagtttt
     3181 tttgtttgaa acaaaaaaat tgcgataatc ttcccaagga tgccagtcgg gtaaatactg
     3241 tcctacatgg tattgata
//
LOCUS       DAZKOK010000181         3236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000181 DAZKOK010000000
VERSION     DAZKOK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.187"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG90W_002773"
     CDS             complement(451..1761)
                     /locus_tag="ACG90W_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0479065.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3131)
                     /locus_tag="ACG90W_002774"
     CDS             complement(1773..3131)
                     /locus_tag="ACG90W_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0479066.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
BASE COUNT         1008 a          659 c          504 g         1065 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct tgtttttata ttcacaatgt tggcttttta
     3181 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataa
//
LOCUS       DAZKOK010000182         3170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000182 DAZKOK010000000
VERSION     DAZKOK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.189"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2378)
                     /locus_tag="ACG90W_002775"
     CDS             complement(<1..2378)
                     /locus_tag="ACG90W_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0479067.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVV"
BASE COUNT          855 a          730 c          527 g         1058 t
ORIGIN      
        1 accaccgctg tctgacttct ttcgatctca aactcgattg gtgtcgcatc taattcatac
       61 cctattggcg cttgagtttc taccagctga tatgctcctg gtgctaaacc gtcaattgcc
      121 agttttccat cttctcctgt agttaaaccg gtttgtagcg tttctccttc tcggttctgt
      181 aactcaaaaa cagctccttg aaggacttca ccactgcctt cagcatgttt aattagttca
      241 acaccacccg tatcaaggat gttttcaatg gttaacgtca cttcttctat ggagtattca
      301 tttataaaaa catcatgtac cgtcgcatct aaaagatacc cttccggtgc ttctgtttct
      361 cggacttgat agctacctac aggaagcggt tcacttaaaa attctccgtt ttctgccgtt
      421 ataccttctt gtatcacttg tgaatcgctt gataaaattt caaattttgc tcctgccagc
      481 ctttggccat tctcatcagt tttaattact ttaatacgcc catcgatttt ccttgttctt
      541 acagtactcg tattcaatcg attgtcacta ttcacaagcg ttgcaaagtt taaaatttga
      601 tcgccgaccg catacgcctc tacattgacc ttaaaagaga gggtaaaact tgtcggacca
      661 ccaatcgtat tattcgcact gaaacgaact attccattat tgacttcggc atcaatgaaa
      721 gagggaatat ctgatagaaa tgacacaaat tccatgcctt cttccaaaac atcttcaata
      781 acaagcgtat ttcttgttcc agaaaacatc gagacatgca gagtatacgt tagttcattt
      841 tcattgagat tgataacatt tttgtccaca ctttttctta aagagattgc cggaagatta
      901 ctttcaggga ttctcaagcg gtagtctcgg ctcattacct gattattgta ggtcatactc
      961 gctcgattcc gagaaaaccc atcctcttgt cttccttcat tgaacaagtc gacatcggtc
     1021 acttcaagag catattcaat aaaatacgat tgaccctctg ctagattacc aaagtccaca
     1081 acaagtgtat tcgtttcctt attaaaagat actttgtcca caaattcctc ggaaacataa
     1141 tgagttctaa cgtttgcttc acctggattc gttacttctg cgatccgaac cccacctgaa
     1201 ataggttctc tatttccagt ggaactccat gtagtcggat tcggtgccaa agttgttcct
     1261 tgcggtagta catcggtaac cactacaccg tcttttcgtg atccttcata atttacctgc
     1321 aaaataaatt tattactgtt ggtcacgtct tgcccgtcaa tcagcagatt cccaagattt
     1381 ccttggctgt cgatttctct agagccatag tataatttcg aaaaaatggg tcttgatgaa
     1441 ggaataggcg taattgaaaa ttccgtgttt gctgctcgct cagcataagt aagactcact
     1501 tgaaagtcat tatttggctc agtaccgcct gattgaaaga aagcaacttc aaacgtgata
     1561 ctgaatgtat acgactgagc ggcttcattt aatccttcat aatcaaaggt aaatggaaaa
     1621 acaacgtctg tgccttcttg gtaaaatgcc agtggcttta aaggtgaatc atcagcgacc
     1681 tccactctaa tgaactcaat taagccaatc gatgctctag gaactcgtac gaccatctcc
     1741 ccatattctt caagaatatc gccagcgcct ccttgaatgt atccctttaa cgtttgtgta
     1801 tgcctgctcg gcaaagtggg gcgctcagca gagatgccga tgtcataatt caagagctcc
     1861 tcttgtcttt caatctgaga ttcctgcgtt atagatggct cagatgatct ggcttcatta
     1921 aaagattcac ttaagctttc ttctggtata ttaaaagatt cacttgtgct ttcttctggt
     1981 acattaaaag actcacttgt gctttcttct ggtacactga tttctattga gaccgtattt
     2041 gattgaatat caccgcttct tgcggtcaat tcgtatttac ctgaatgctg ggaagaataa
     2101 gtaatcagac gttcgtaatc attgagttgc ttcacttccc cttcaatcaa atcctcactc
     2161 ttatcaatga ccaatgcatt tagcaggtct ggtgttcctc tgactcgcaa ctggaactgc
     2221 tcacccaaag cagcctcctc ttgatcaact tcgatcgtca gcgaacgctc tggctgttct
     2281 gctaaagact cagctagaac cgttatgttc ccaaattgat tgatcacaat aagcataact
     2341 agaaacatac ttaacagttt atgaaatttg ttcctcacta ttactccccc ttataatttt
     2401 gactaaacaa caaggtaccc gataattttc taaaaagatt ccccaactca aaatggctgc
     2461 gttacctccc tcgtattttc ggttcaactc aagcgttgca atgtaagctg taaacaaagc
     2521 taatgattgc cgattgtctt ctgttgattg attcgatagc tttgtatgac ttttagttgt
     2581 ccgtgtggct taaccataat tatgatttca cgattcctcc tgccagtgcc ttgcacaagc
     2641 tggagcgtaa ccctaatagt tattttgtta atgtacttat atacttttat acttttccat
     2701 tagaaacaat cactaaatcg ttgataccaa ggtcagactc tagaatttca tcagtgagtc
     2761 cctgtattgg ttaaccttca tcaatcccaa aaaaatatat caatgcttag catcaaaatg
     2821 aatatatagt ttagaatact tgggattcat cgataactga tcgcttattt ggccatatta
     2881 tttggcactt ggttcaacta aacgcattta ggatgttcct tcattctcgt catggatgtt
     2941 ctcaaaccgt cattactaat aaactgtcca ccgaatcgat ggttcatttc ttgcattttc
     3001 cacgtccaat tactcttttt gctctttatt tgggcgtagt ttcactttct taacttgtat
     3061 cgtcttccat caactcctta atcatcttct tagctttatt ggctcggttt ccttgtaaaa
     3121 ggtatctaca aatagtgaca atctgtatta aatcttcatc attttctgta
//
LOCUS       DAZKOK010000183         3126 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000183 DAZKOK010000000
VERSION     DAZKOK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.190"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1040
                     /locus_tag="ACG90W_002776"
     CDS             72..1040
                     /locus_tag="ACG90W_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0479068.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1050..1538
                     /locus_tag="ACG90W_002777"
     CDS             1050..1538
                     /locus_tag="ACG90W_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0479069.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            1834..2115
                     /locus_tag="ACG90W_002778"
     CDS             1834..2115
                     /locus_tag="ACG90W_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479070.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            2117..2533
                     /locus_tag="ACG90W_002779"
     CDS             2117..2533
                     /locus_tag="ACG90W_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479071.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1251 a          543 c          475 g          857 t
ORIGIN      
        1 attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag ttaatcttga
       61 aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat acgattcctt
      121 tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct agaatgcgtg
      181 atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta agcaattata
      241 aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac aaattaatgg
      301 atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt gttttattta
      361 atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa gtccatgaga
      421 aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc caacaattca
      481 atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa tacagaacca
      541 aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc cctaaaagtt
      601 atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa
      661 tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag cctgtcattg
      721 gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac aaacatgctc
      781 caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt caatctgaaa
      841 aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta gaacttttac
      901 aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt aacaaacaaa
      961 tagcagataa caattccgat attaccccag aatttaaaga aaacttacta gataatctcc
     1021 aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa gaattagcag
     1081 atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta cctagtaatt
     1141 ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca acaattacaa
     1201 aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat tcattaccta
     1261 ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa aaagataggc
     1321 aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac cagcaacaac
     1381 aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc cagctaacct
     1441 atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact caaaaaggac
     1501 aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt tattcataaa
     1561 aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat ctcacgtcgc
     1621 tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt gctaggtagg
     1681 ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca ttgtggatag
     1741 cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt atgatataca
     1801 taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg gaatattgat
     1861 ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa aactggcaaa
     1921 aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga ccaggcactt
     1981 aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga tatacaagca
     2041 aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat tgaaacactt
     2101 atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc tttatcccag
     2161 tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata actactcgga
     2221 agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa ctaccagcaa
     2281 ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa tcattgcaga
     2341 atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtata
     2401 tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa atcaacccaa
     2461 aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc caacaaactt
     2521 tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct tttttagggg
     2581 gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag aaaaatccgc
     2641 ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa tatcggttat
     2701 ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa gactgctcta
     2761 acgagcagcc aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc gcttatgaag
     2821 cgctgccaaa aatttaccat attttatgaa aaataacaag tttctaaaaa ggaatcggtt
     2881 aagattcaca aagaatcgta aacagacaat agcggaaaat caagcttccg aacgtacctt
     2941 ttagctagac caatgaaaaa tcacaaagtg agcccctgta tcggttgata caagggctac
     3001 ttggcatact cgacattatg tctactagct cgataaataa cgattatgtt cccaaaacaa
     3061 tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa ttataggtgt
     3121 atatca
//
LOCUS       DAZKOK010000184         3102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000184 DAZKOK010000000
VERSION     DAZKOK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.191"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            776..>3102
                     /gene="esp"
                     /locus_tag="ACG90W_002780"
     CDS             776..>3102
                     /gene="esp"
                     /locus_tag="ACG90W_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0479072.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1133 a          460 c          602 g          907 t
ORIGIN      
        1 ttattctttt tgatacatga tgaatttatg gattatcaaa aattggatca gttaattgaa
       61 agagaaagct gggaagatta ttacgtgggt ctaacttttt cagaaaagtt aaacaactac
      121 aagttaatag attataagta tttgagtgaa agcaattaat tattcatgta gagtactaga
      181 cactttaaat atggacaatt caattataaa gaaaaagaat caataattta atgaggctgt
      241 gacaaatgtc attttttgtt tcgctaaaaa cgagtgaaaa aatgttgata actccaatca
      301 ttcgtttaag attctaggaa tcattcagaa atgctgtgaa accgatactt tataattata
      361 aagaaggtga tttcaaattc cgaagaagga atttgaagtc acctttttac ttatgtcaca
      421 gtctcattca gtaatgcggg ataaggctcc ttattccgtt atttgaatac tctgaagttt
      481 aatctaagaa agggtaatat tttgaaaaca tgcttcatcg catagaaatg aacttaattt
      541 cagaaagaat acatacatat ctttttatat gttttcagct gctgaatggt taaaaagcac
      601 aaatatccga aaaaaacatg aagaaatgta ctttttttgt tgtaatgttc atgaaattct
      661 ctaagtaaat tagttaacat aaggttagta agattcctat tctgtgaata atacgtatca
      721 gatttatctt ttattcttac taggaaagta attattaagg gagttgattt gaataatggt
      781 tagcaagaat aataagagag tatttctaga aaaaacaaag aaacgagtat tgaagtacag
      841 tatcaaaaaa ttaagtgttg gggttgcatc tgttttgatt ggtgttggtc ttgtcttggg
      901 aacaactgaa ctagttaaag cacaagatga aatttctcca agcagcccac ttgaaacagc
      961 gataagctcc gtacaagtag gtgacaaagt agcttcgggg aatacttttc aggagaatcc
     1021 tggttatacg aaaaattata acttttcgga tttacaattt agtcctcaag aactaactgg
     1081 agacacacta aaaggcaata caattggatt tgaagtttac ggaaaacata atattgcagc
     1141 atcaactaaa aactgggaaa tccgtcttca attagatgaa cgattagcta aatatgtaga
     1201 aaaaattcag gtcgatccaa agaaaggaat tggatcttct agacggacct ttgtaagaat
     1261 taatgattcg ctgggtagac ctacaaatat ttggaaggtc aattatattc gtgcaagtga
     1321 tggattattt gctggggcag aaacaaccga tacgcaaacc gctcctaatg gggtaattac
     1381 gtttgaaaag agcctagatg aaatttttaa agagataggc atagacaatc tcaagaccga
     1441 tcgtttaatg tatcgaatct acttggtaag tcatcaagac gatgataaaa ttgtaccggg
     1501 tattgatagt acgggctact ttttaacaga ttcagatgat ttctataata gcttagatgt
     1561 atctgaaaat aatcctgacc agtttaaaca tggatcagta agtgcaaaat atgagaaacc
     1621 taatactcaa acaaaagatg gttcaggatc cacaggtgct aatggagcta taatcttaga
     1681 tcataagtta acgaaaaatt ataatttctc atattcatct tccgcaaagg gcacgccttg
     1741 gtatgctaat tacaaaattg atgaacgatt agttccatat gtagcgggta tacagatgca
     1801 catggttcag gctgacaaag taacatatga tgtttcgttt gaatccggaa caaaagtagc
     1861 tgatttagca atcgagcgac gggaaggtca tgaaaattat ggcataggtt caataactaa
     1921 taatgattta actaaactta tcgattttgc taatgcaagt ccacgtccag tcgttattcg
     1981 atatgtttta caattgacta agccattaga tgaaatctta gaagatatga aagcaacagc
     2041 acaagttgaa gaaaataaac catttggtga agatttcatc tttgattctt ggttgtcgga
     2101 tacgaataaa aaattaatcc agaacactta tggaacaggt tattattatt tgcaagatat
     2161 tgatggtgat ggaaaccctg acgataaaga agagagcgga gacacgaatc catatatcgg
     2221 gaaacctgaa ttagaagaag tatatgatgt tgacacaaca gttaagggga aagtattcat
     2281 ccacgagtta gcgggaacag gtcacaaagc ccaacttgtt gataaagaag gtactgtatt
     2341 agcagaaaaa actatcgctc caaatgaaaa agatggggct ccaatttcag atactgtaga
     2401 atttgaattt acgggtgtag attcaagtaa attaatcgcg aaagatgaat taaaaatcca
     2461 aatcgtttct ccaggttttg ataaaccaga agaaggttca accgttatta aggaatcacc
     2521 aaaagcggtt gataaacaaa ccgtggtagt tggatttaaa ccagatgcta aagaatcaat
     2581 tcggaataat aaaaacttac ctgaagatgc agagtattca tggaaaacag agcctgatac
     2641 ttctaacgtt actgatagta cgaaaggtat tgtaactgtt aagatcggaa atcgaacttt
     2701 cgacgtggat gtagagtttg ctgtaaaagc ttctcaagct atggaaaatg atgcaacata
     2761 cgtacctata acaacaactc cagaaacgac agttcaaagt ggaaaaccta catttgataa
     2821 accagatgtt cctttagcta aagatgcctt ttcaatttca gatgtctata ataagtactt
     2881 cggtaatgca agtgttgatg caaacactgg tattgttaca ttcactccag ctaaaggtgt
     2941 aggagaatcg gagccgatta ctggaacaat tcctattaaa attgtttacc aagatggttc
     3001 tgtaggcacg accgatttag cagtaactgt aagtaaagat atttatgaaa atccaggaga
     3061 aaacattcct gcaggctacc acaaagtaac cttcaccgca gg
//
LOCUS       DAZKOK010000185         3070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000185 DAZKOK010000000
VERSION     DAZKOK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.192"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            286..1101
                     /locus_tag="ACG90W_002781"
     CDS             286..1101
                     /locus_tag="ACG90W_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479073.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1227..1484
                     /locus_tag="ACG90W_002782"
     CDS             1227..1484
                     /locus_tag="ACG90W_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479074.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1736..2308)
                     /locus_tag="ACG90W_002783"
     CDS             complement(1736..2308)
                     /locus_tag="ACG90W_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0479075.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2324..2929)
                     /gene="fic"
                     /locus_tag="ACG90W_002784"
     CDS             complement(2324..2929)
                     /gene="fic"
                     /locus_tag="ACG90W_002784"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0479076.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1017 a          539 c          473 g         1041 t
ORIGIN      
        1 aataagaaat tcaaaaaaga aaaaactttc cttaaaaaca aaaaatagga gtattcagtt
       61 tcttaatagt tttgtaatag ttgtatgtag aagttttctt aatagttttt gtaataattt
      121 attaatcgta gtttatataa cttatttgta agtgactaga gaattaaata aaaaataatt
      181 actatctagt acgaaattaa ttacatagga taatatatta aggtgctgga ctacaagtaa
      241 aaaatactgt atgatcatga cagattcaag aaaggagcaa taagattggt taagatagca
      301 ggactttcta aaggtggaaa aaccgtttca caactttata aacaaaacta cagagactgg
      361 agacagttca gaaaaaacat taaggattct tactttattc taccaacaga acttgaaaaa
      421 tatttaccga ataacaaagc tattaattta tatctgtatt actgttttaa ggcaaagaat
      481 gaaggcgggg attcctggca ttcggtcaat accatagcag agtctctagg agtgacaact
      541 aaaactataa acacctggaa taaaacctta gttgatttag gtatgattgc acgtatagac
      601 gataaccatc tcagtaaatc gacaatttta cttcctctct ctagttacta taaattagcc
      661 cttgatagtt ctttaaagga tgttaccgag agcactgtcc atgaaattga tggcaacttg
      721 gttgcagttt atcatttgtt tgaatggaga aaaaatgagg ctgatgaaaa ttataacgtt
      781 ccttataaca cactctgttg tgtttttaaa agaaaaacac aaaaagatac tatctataaa
      841 tttatcttgg tccaagatga gaaaataagt aatttcgaat taagcactcc ttctagtaag
      901 atttatgacg atgcatatag atttgaatgt caagatctag aagccctcct ttccgcaaat
      961 aatctaaaca atatagaaca tgaaaacttt gttgtaaaca ctagatttaa tttagtttct
     1021 gaaaaggact cagatttgtt agatttgctg atagagctta ctaaaaatct agataagaaa
     1081 gaaaacatta ctgtactata actagcatcg gtccatcatt aagtttggac tattagaaca
     1141 cttgctatta cagaaaaagg tatgctagcc acgcttacta tttattggta tcaaacaagt
     1201 atcttaattt gaaaggaaga tgatttatgg ctaatttata tatagagaac aaaggagact
     1261 tgaatgccat tattacttta atcgtagtta tggtaaaatc aataggtaat atcacaatag
     1321 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
     1381 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
     1441 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
     1501 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
     1561 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
     1621 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
     1681 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1741 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1801 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1861 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1921 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1981 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     2041 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     2101 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     2161 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     2221 tctctacgcc gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     2281 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     2341 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     2401 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     2461 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     2521 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     2581 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     2641 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     2701 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2761 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2821 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2881 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2941 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     3001 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
     3061 cacagtctag
//
LOCUS       DAZKOK010000186         3038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000186 DAZKOK010000000
VERSION     DAZKOK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.193"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG90W_002785"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG90W_002785"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0479077.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG90W_002786"
     CDS             complement(1034..1954)
                     /locus_tag="ACG90W_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0479078.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG90W_002787"
     CDS             2067..2819
                     /locus_tag="ACG90W_002787"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0479079.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DAZKOK010000187         3031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000187 DAZKOK010000000
VERSION     DAZKOK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.194"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..1763
                     /gene="alsS"
                     /locus_tag="ACG90W_002788"
     CDS             99..1763
                     /gene="alsS"
                     /locus_tag="ACG90W_002788"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0479080.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1776..2486
                     /gene="budA"
                     /locus_tag="ACG90W_002789"
     CDS             1776..2486
                     /gene="budA"
                     /locus_tag="ACG90W_002789"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0479081.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2616..>3031
                     /locus_tag="ACG90W_002790"
     CDS             2616..>3031
                     /locus_tag="ACG90W_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0479082.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDE"
BASE COUNT         1009 a          504 c          690 g          828 t
ORIGIN      
        1 ctatactttt gaatgaaaca ggtttagaaa ctgtatcaat tatttgtgat tatttctaac
       61 taataattaa aaataaagca taaaaaggac gatgttcaat ggacgaaaaa atgaaacaag
      121 gttcgaaatc tgtagtagaa agtttgatta atcatcatgt tgattatgta ttcggtatac
      181 caggtgcaaa aatcgatggt gtatttaatg aattagagga tcaaggtcct gaattaatcg
      241 tgactcgtca cgaacaaaat gctgcattta tggcacaagc aatcggacgt attacaggag
      301 agccaggagt agttatcgca acaagtggtc ctggtgctag taacttagct acaggtttgg
      361 taacagcgac agcagaaggt gacccagttt tagcaattgc cggacaagta aaacgaagcg
      421 atctattgaa gttgacccat caaagcatgg ataatgcagc actgttccag ccaatcacaa
      481 aatacagtgc agaaatccaa gatccagaaa caatttcaga agtgattgcc aatgcttatc
      541 gtatggctaa gagttcaaaa aaaggcgcga gctttatcag tatcccgcaa gatgtagtgg
      601 atgcacctgt ccaagggaat gtcatcaagc cattgagcga tcctaaatta ggttctgctt
      661 ctgcagatga tatccgttac ctagctgaac gcattagaga agcaaaacta ccggtactat
      721 tagtaggtat gcgaggatca agtgaaaaag aaacgttagc catcaggcaa ttagttggaa
      781 agacagcatt gcctgttgta gagacattcc aagcagctgg agtgatctca cgtgaattag
      841 aagcgcattt ctttggtcgt gtgggtttat ttagaaacca accaggagat atgcttttga
      901 aacgaagtga tctagttatt gctattggct atgatccaat cgaatatgaa gcaagaaact
      961 ggaatgcgga aaaagatgcg cggatcattg tgatcgatga agcgccagct gaaatcgatc
     1021 cttttatgca gcctgaaaga gaattgatcg gtgatatctc agccactctt gatttattga
     1081 caggttcatt agagcctcaa caagtgtcag aagatgcaaa agaatattta gcatctcttc
     1141 aagcagaatt aacggagcgg gacattgttc aatcaaaagg agaagcaggt atcttgcatc
     1201 ctttagaagt tattaacacg cttcagtcaa aagtaacaga tgatatgact gttaccgtag
     1261 atgtaggtag tcattatatt tggatggcac gccactttag aagttatgaa cctcgtcatt
     1321 tgttattcag taatggtatg caaacgcttg gtgttgcatt gccatgggcg atttctgctg
     1381 cattggtgcg tccaaataca caaatcgtat cagtttccgg cgatggggga tttttattct
     1441 ctgcccagga tttagaaacg gcggtacgta aaaaattgaa tatcgttcat ttgatttgga
     1501 atgatggaca ctacaatatg gtagagttcc aagaaaaaat gaaatatcag cgggcgtcag
     1561 gtgtagactt tggtccagtt gattttgtga aatatgcaga agcttttggc gctaaaggaa
     1621 tccgagctac aagtgtagag gaattagaaa aagctttaga agaaggattt gcgacagaag
     1681 gacccgtaat tattgatatt ccgattgatt accgtgataa tgagaaattg ggagaaacaa
     1741 tcttaccaga tcaattctat taaggaagtg tcgacatgac tgaaaaaatc ttataccagc
     1801 acgggacatt aggagctttg atggctggct tgatggatgg aacagaaacg attgctcata
     1861 tattagaaaa aggaactctg ggattgggaa cattgcatgg gcttgatggt gaagtcatct
     1921 ttttagatgg cgtagcttat caaggaagat cagatggctc agttgcccaa ttagatggaa
     1981 gtgaacttac cccctatgca gcaattacag attttacgcc agatactagt ttttctgtct
     2041 caaaaactgc agatggagaa caattgaaaa aagacatatt agtaagagaa gccggggaaa
     2101 acctattttt ggcagtgaag ataactggat tattcaaaaa tatgcacatc cgtatcatgc
     2161 cgaagcaaga aaaaccttac agaagactag caaaaatttc agagtctcaa ccagaattcc
     2221 aacaaagcaa tgtccaagga acattagtcg ggttctttac gcctgaatta ttccaaggag
     2281 ttgctgcagc gggatttcac ttgcatttta tcgatgatac acatacattt ggcggacatg
     2341 taatggattt tgaagtggct gaaggaaaag ttgagattag ccgcatctcg tcccttgtac
     2401 agcattttcc agttgatgat ccgacttttg ttcacgaaac gattgattat gctgatttag
     2461 cagcagaaat ccaacaagca gaatagaaaa agacagcgag atgcgttcaa ggtgattaac
     2521 ctgacgtatc tcgttttttt gaggaggaaa cctagcgttt acaaaaaaac tgagctataa
     2581 tcttctccat agatcaaaaa ggagcggttt ttatcatgtg gacagaaaaa cagaaaaaag
     2641 atgcatacga aattttatta ttacagcaac aaggattatt agaagcagaa gataactggt
     2701 tagcaaattt agcaaattca tctgcattgt tgaatgagac tttgcctgag actgtctttg
     2761 caggatatta tttattcgat ggtaatgaat tgattttagg accgttccaa gggcgagttt
     2821 cttgcacacg tatcaagatg ggaaaaggag tttgtggaga atcagctgaa aaacaagaaa
     2881 cattgatcgt tgatgatgtc aaaacccatg caaattacat ttcctgcgat tcagccgcac
     2941 aatcagaaat cgtagtgcca atggtaaaag aaggtaattt aatcggtgtt ttagatatcg
     3001 attgcggagt aacgaaaggg tatgatgaaa t
//
LOCUS       DAZKOK010000188         3025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000188 DAZKOK010000000
VERSION     DAZKOK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.195"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG90W_002791"
     CDS             complement(287..649)
                     /locus_tag="ACG90W_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479083.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG90W_002792"
     CDS             complement(723..1103)
                     /locus_tag="ACG90W_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0479084.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG90W_002793"
     CDS             1185..2360
                     /locus_tag="ACG90W_002793"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0479085.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG90W_002794"
     CDS             complement(2437..3021)
                     /locus_tag="ACG90W_002794"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0479086.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          578 c          499 g         1006 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattg
//
LOCUS       DAZKOK010000189         2932 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000189 DAZKOK010000000
VERSION     DAZKOK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.196"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..984
                     /locus_tag="ACG90W_002795"
     CDS             88..984
                     /locus_tag="ACG90W_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0479087.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1076..1291
                     /locus_tag="ACG90W_002796"
     CDS             1076..1291
                     /locus_tag="ACG90W_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0479088.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1309..1581
                     /locus_tag="ACG90W_002797"
     CDS             1309..1581
                     /locus_tag="ACG90W_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0479089.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1583..2446
                     /locus_tag="ACG90W_002798"
     CDS             1583..2446
                     /locus_tag="ACG90W_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0479090.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1163 a          464 c          510 g          795 t
ORIGIN      
        1 aaagagggct tttatatatt aatcacaaat cacttatcac aaatcacaag tgattaatca
       61 caaatcactt gtgatttgtg attcttaatg atacaatatt actatacaaa aaaagaatgg
      121 ggcgtagtta tggagaagga aaaactaaaa atacttgaag aattaagacg tattttaaac
      181 aataaaaatg aagcaattat tatcttgaat aattacttta aaggtggtgt tggaaagtcc
      241 aaattatcga ctatgtttgc ttacttgaca gacaaattga atttaaaagt tttaatgatc
      301 gataaggact tacaggcaac attgacaaaa gacttagcaa aaacttttga ggtagaattg
      361 ccacgtgtca atttttatga aggcttgaaa aatggaaact tggcttcttc tattgttcat
      421 ttgactgata atttagactt gatccctggc acgtttgatt tgatgttact gccaaaatta
      481 actcgctcat ggacttttga aaatgaaagt agattgcttg ctactctttt agcaccttta
      541 aaaagtgact atgatctcat tattattgat actgtaccaa cgccaagcgt ttatacaaat
      601 aatgcaatcg tggcgagtga ttacgttatg atccctttac aagcagaaga agaaagtaca
      661 aacaacattc aaaactatat ttcctatttg attgatttac aagaacaatt taaccctgga
      721 ctagatatga tcggttttgt tccttattta gttgatacgg acagcgcaac gataaaatca
      781 aacctggaag aactgtacaa gcaacataaa gaagataact tggttttcca aaatattatc
      841 aagcgaagta ataaagtaag tacctggtct aaaaatggaa ttacagaaca caaaggctat
      901 gacaaaaaag ttttatccat gtatgagaac gtattttttg aaatgcttga gcgaattatt
      961 caattagaaa acgaaaaaga atagaatcac aaatcacaag tgattaatca caaatcactt
     1021 gtgatttgtg attgttgatg ataaaataag aataagaaga aatagaaaga agtgagtgat
     1081 tgtgggaaat ttaggcgcac aaaaagcaaa acgaaatgat acaccaatca gtgcaaaaaa
     1141 agatataatg ggagataaga cggttcgtgt tcgtgctgac ttacaccata ttataaaaat
     1201 tgaaacagca aaaaatggcg gaaacgtaaa agaagttatg gatcaagcct tagaagaata
     1261 tatacggaaa tatttacctg ataaacttta aaaaggagtg aaaatgaaat ggcagttacg
     1321 tatgaaaaaa catttgaaat agagatcatt aacgaattat cggcaagcgt ttataatcga
     1381 gtattaaact atgttttgaa ccatgaacta gatactaaaa atactcgttt actagaagtg
     1441 aatcttttaa atcaattaga agtggcacaa gaagttgatt tatttcaaca accatttgaa
     1501 gaattacaag ctattcatga gtattggcgg tcaatgaatc aatattcaaa acaaattttg
     1561 acaaaagaga aagtggctta acatggcgaa tattactgac ttcaccgaaa agcaatttga
     1621 agatcgttta gaaaagaatg ttgaacgact aactaaaaat agactagcgg ttgaatcgcc
     1681 aaccgctttt ttacttggtg ggcaaccagg atcaggtaaa actagtttgc gatcggcaat
     1741 ttccgaagag acacaaggaa atgttgttat cattgataat gatacgttca aacaacagca
     1801 ccctaatttt gatgaactag tgaaacttta tgaaaaagac gtagtaaaat acgttacccc
     1861 ttattctaat cgcatgacag aagcgatcat aagccgtttg agagataaag ggtataattt
     1921 agtgattgaa ggtacaggac gaacaacaga cgttcctatt caaaccgcaa caatgcttca
     1981 agccaaagat tatgaaacaa aaatgtatgt catggcagta cctaaaatca actcatattt
     2041 aggaacaatt gaacggtatg aaactatgta tgcagatgat ccaatgacag ccagggcaac
     2101 accaaaacaa gcgcatgata ttgttgtcaa aaacttaccg accaatttag aaacccttca
     2161 taaaacgggc ttatttagcg atataaggct ttacaataga gaaggagtga aactctattc
     2221 aagcttagaa acgccttcca ttagtccgaa agagacctta gaaagagaat taaatcgtaa
     2281 agtatcaggg aaagaaattc aaccgacttt ggagagaata gagcaaaaaa tggttcaaaa
     2341 tcaacaccaa gaaacccctg aatttaaagc aattcaacaa aaaatggaaa gcttgcagcc
     2401 acctacacca ccaataccca aaacacctaa acttccaggg atttaaacct taaaaaagta
     2461 aaagagtagt taccaaaaaa cggtaactac tcttttttta taaaaacatt tcctcatttt
     2521 ttaaaagctg atcgagaaat aaagcacaca actgtattct ttcttttttc agttggtaca
     2581 ttttgcagtc tgtaacgtgc catttactgt ttgttacctt aacatatcca gtcgaaaaca
     2641 caagggtata taaacgagca agctcgccct tgtgttttct ttgtgtctat gttgcgttct
     2701 cgtcacagca aacgacacga acacactggc gaaaatgaac caacaaaacg aaaaaagaaa
     2761 ggagcgtaca ctgtaattta tttctagctt ttaaaacatg aggaaaacag cttcacttcc
     2821 tcaaacgcct aacaaaaatg gttgtcaaaa aactaaaaat caaaaaaaga aaagaggaaa
     2881 agaaaatgga aaaaacaaaa aaattacaat tagaagattt tacagaaaat gg
//
LOCUS       DAZKOK010000190         2810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000190 DAZKOK010000000
VERSION     DAZKOK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.197"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1110)
                     /locus_tag="ACG90W_002799"
     CDS             complement(70..1110)
                     /locus_tag="ACG90W_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0479091.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1459..1785)
                     /locus_tag="ACG90W_002800"
     CDS             complement(1459..1785)
                     /locus_tag="ACG90W_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479092.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1772..2560)
                     /locus_tag="ACG90W_002801"
     CDS             complement(1772..2560)
                     /locus_tag="ACG90W_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0479093.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT          824 a          465 c          387 g         1134 t
ORIGIN      
        1 ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct
       61 ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc
      121 tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata
      181 aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt
      241 cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata
      301 aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac
      361 ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc
      421 tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac
      481 ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt
      541 agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga
      601 ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc
      661 cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt
      721 aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc
      781 aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc
      841 taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc
      901 ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga
      961 ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat
     1021 gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc
     1081 ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt
     1141 tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta
     1201 ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt
     1261 accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta
     1321 tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag
     1381 cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat
     1441 cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat
     1501 caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt
     1561 gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg
     1621 aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt
     1681 cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc
     1741 tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt
     1801 cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa
     1861 aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat
     1921 aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt
     1981 ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct
     2041 tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt agtcctttta
     2101 atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga
     2161 ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga
     2221 ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg
     2281 cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat
     2341 ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta
     2401 tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat
     2461 ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa
     2521 ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc
     2581 ttatattata atataattat atcataatat tgtgatattg caatatcgta tatgcattat
     2641 attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt
     2701 attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa
     2761 tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
//
LOCUS       DAZKOK010000191         2795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000191 DAZKOK010000000
VERSION     DAZKOK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.198"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..1556
                     /locus_tag="ACG90W_002802"
     CDS             231..1556
                     /locus_tag="ACG90W_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0479094.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1549..1899
                     /locus_tag="ACG90W_002803"
     CDS             1549..1899
                     /locus_tag="ACG90W_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479095.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1881..2066
                     /locus_tag="ACG90W_002804"
     CDS             1881..2066
                     /locus_tag="ACG90W_002804"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479096.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT          986 a          442 c          539 g          828 t
ORIGIN      
        1 ttttctaata taactaagca aaactggata atacgacact tattttcaaa ttttcacaag
       61 actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac aaatcaacag
      121 aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc gattcttctt
      181 tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt atgatggatt
      241 attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt tacgcaagtg
      301 tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc atgagtcgag
      361 cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag aagaagtacg
      421 gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa acgttacatg
      481 tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac aatattttca
      541 gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg atcttaaaag
      601 tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg agaaagaagc
      661 tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct acagtaggtg
      721 tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa cataatgatg
      781 gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat attcctgaaa
      841 tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa attggtatca
      901 tgagtattaa agacttagct aattggaatc catacactat taagagccat ttaggtgtga
      961 ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata gcgatcccac
     1021 cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga gattatacca
     1081 ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct attcgtatca
     1141 ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg atcttagaaa
     1201 cgagaccagg tttttcacac cagatgaaga tccctataac agataataca aaggaattgc
     1261 agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta aggcacgttg
     1321 gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta tttagtgatc
     1381 cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat
     1441 atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt agtattacta
     1501 gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat gattgattat
     1561 aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac tgaaaaaatt
     1621 gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat gacgactgaa
     1681 gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg tattcaaaaa
     1741 gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat cttaggtagt
     1801 gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat acgtcatatc
     1861 gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag caatatgagc
     1921 taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc cagcgtttga
     1981 taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca ggataccata
     2041 aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt tatgttttac
     2101 attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa aaaaactagt
     2161 tacaacctta ggacgttgta actagcttga agcatactag agctaactaa aatagcacta
     2221 gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag cttattcagc
     2281 taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac tacgaataac
     2341 gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac taaacattgc
     2401 gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag ctttatgatg
     2461 ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa ttctcctttt
     2521 taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc atttgattga
     2581 gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt ataacataaa
     2641 tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca aacgatttat
     2701 tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat gtttgttaaa
     2761 accaccatta cctgtagata aaaaccaata tattt
//
LOCUS       DAZKOK010000192         2575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000192 DAZKOK010000000
VERSION     DAZKOK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.199"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1408
                     /locus_tag="ACG90W_002805"
     CDS             <1..1408
                     /locus_tag="ACG90W_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0479097.1"
                     /translation="TVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNT
                     PGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVT
                     NLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNAD
                     KYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYP
                     DGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTW
                     KEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGK
                     TEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVV
                     VSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLI
                     LLGTLGLLGYKNRKKENE"
     gene            1542..>2575
                     /locus_tag="ACG90W_002806"
     CDS             1542..>2575
                     /locus_tag="ACG90W_002806"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0479098.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNL"
BASE COUNT         1010 a          358 c          518 g          689 t
ORIGIN      
        1 aacagtagaa ggcgaaaaag tagaagttgg gggcacagta gatctaacag ataatgttac
       61 taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat
      121 tgataccaac acaccaggta attacgaagg tgtcattgaa gtaacgtatc cagacggtac
      181 gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctgacg ccgataaata
      241 tgaaccaaca gtagaaggcg aaaaagtaga aattggaggc acagtagatt taacagataa
      301 tgttactaac ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg
      361 tacaattgat accaacacac caggcaatta tgaaggtgtc attgaagtaa cgtatccaga
      421 cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt ctaatgcaga
      481 taaatacaat cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc
      541 tgagggaatt aagaacaaag aagatttacc aaaaggaact acttatgctt ggaaagagaa
      601 agtagatgtt agcacagctg gaaataaaaa aggtactatt gtagtgacat atccagatgg
      661 atcatctgat gaagttgaag tagacgttac agtgacagat actcgttccg atgcagataa
      721 atataatcct gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga
      781 gggaattaag aacaaagaag atctaccaaa aggaactact tatacttgga aagaaaaagt
      841 agatgttagt acagctggaa acaaaaaagg tactgtagta gtaacatatc cagatggatc
      901 taaagaagaa gtagaagtta ctatttctgt agtagataaa aaagcaccga ataaacctca
      961 agttgatcct attacagagg gtgaccaaat tgttactggt aaaactgaac caaatgcaga
     1021 ggtgacagtt acattaccag acggaagtca acaccatggg acagctgata aaaatggtaa
     1081 ttttacagtt aaagttccta aattagaggc agggacaaaa gtgatagtaa ctgcaactga
     1141 tgaatctggt aatactagtg aacctactaa tgtagttgtt tcttccaaca aaaaagacag
     1201 tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga
     1261 caaaaataga ggcaaaagcc agtcctcaaa agtttttcct aagacgggag agagtgacag
     1321 taatatcttt actattagcg gtggtttaat actgttagga acgttaggat tattagggta
     1381 taaaaatcga aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc tctgagaaga
     1441 ggaagaatct ttttcttcct ctttttgctt ttttatgatg ggaaataagt gttagttaaa
     1501 gatgaatata ctttttatag aaaggaaaag aatgtaatta agtggaaaaa atagtaatta
     1561 ttggttcaaa ccattcaggg ctagccgcag ctcgatttct aaacttaagc gattcaaatt
     1621 atgaagttat tctaatcgat aaaaaatcag aaattagtta cttggaatgt gggacatcgt
     1681 tatttataaa aggagaagta agtaatttag aagatctttt ttatcaaaat agaatgacac
     1741 taaattctga gataaaaaag ttatttctag agacagaagt tgacaaaatt gatattcaaa
     1801 ataaaaagat tagttgtgta aaaaaagatg gtacaagatt ctctcaagaa tatgacaaag
     1861 ttatattagc tacaggttca aagcaaatta tgctagatat accggggaat aatttagcta
     1921 atattttttc gataaagaat tttccaaatg caaaagagat ttttaaagct ttggatgata
     1981 gtactattaa aaaagtagga attattggag caggatatat tggagttgaa attgctgaag
     2041 ccataagaaa aagagggaaa gaagtatatc ttttcgatgt tgcagataga gtgttgtcga
     2101 cttattacga ccgtagtttt tcagataaag ttgaagaaat actatcaaaa aatggaatac
     2161 atctttgtct atcagaaagt cctaaaaaat atttaggaag aaaaaagatt gaaaaaatag
     2221 tcactacaag tggtacccaa tactctatgg atatggtcgt acaagctata ggatttctat
     2281 ctaatagtcc aattggggaa aaggaactct tgagagatgt tagtggagca tatctaacta
     2341 atgaatacca gcaaacaaat ataaaagata tatatgctat tggggattgt gctacaactt
     2401 tttttacgtc cattaataga agtagtgttg atttttctat ttctaatgct ctaagaacag
     2461 cttatattgc aactcaacat attaacaatc aagattttac accttctggt tcccaattta
     2521 caaatggctt ttctatattt aattacaatt tttacgcagc aggtttgaat ttaaa
//
LOCUS       DAZKOK010000193         2542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000193 DAZKOK010000000
VERSION     DAZKOK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.200"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1168
                     /locus_tag="ACG90W_002807"
     CDS             14..1168
                     /locus_tag="ACG90W_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0479099.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1141..1584
                     /locus_tag="ACG90W_002808"
     CDS             1141..1584
                     /locus_tag="ACG90W_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479100.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          892 a          431 c          440 g          779 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc caagcccttt
     1801 agtgtgtttt gtagaaaggg accttatagt cactttcaca ttacaaacga tacgtcctat
     1861 ggacgtatca aaagggtggg acttatgagc aaattaaagc aaaatttggc tagcaatcct
     1921 tctaaaaaat acacctattg atcagagtca aaataaatca gttttcgagt gagcgaatcc
     1981 aaatttgcaa ctctgaagct ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa
     2041 cattagtcga tatatacata gggctctaat ttgcgtttta aggcgtttta aacgttctgg
     2101 tatgtaatta taccaaagcg actttaaaac gccttaaaac gaattttaga ggttctgtag
     2161 aacgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttta aaaacaagat
     2221 aagggatttt atctagactt gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa
     2281 ggactcctta cgaactgttt ttaagcaaag gagcctttag gcgactgcgc agtttaatgt
     2341 gagagcgtag cgatcacacc tttaaagcga ccgtagggag cagattccca gtttaggaaa
     2401 tttcgcgaac ctgtttggag aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc
     2461 ctcttttata atctctttta taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt
     2521 actctcccaa ggtgtgcaga at
//
LOCUS       DAZKOK010000194         2502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000194 DAZKOK010000000
VERSION     DAZKOK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.201"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..991
                     /locus_tag="ACG90W_002809"
     CDS             104..991
                     /locus_tag="ACG90W_002809"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0479101.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            1077..1307
                     /locus_tag="ACG90W_002810"
     CDS             1077..1307
                     /locus_tag="ACG90W_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0479102.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1364..2329)
                     /locus_tag="ACG90W_002811"
     CDS             complement(1364..2329)
                     /locus_tag="ACG90W_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0479103.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT          841 a          401 c          491 g          769 t
ORIGIN      
        1 tcatttaaaa gataaaattc aaacattttt tatgaaaaaa tccttgtttc tagcgtatac
       61 tagcatagaa gcaaggattt ttgattttta ggaggtcgaa aaaatgaaac ttggttttat
      121 cggaacaggc gtcatgggaa gtgctgtcgc acgccatcta ctagaagcag gacacgaagt
      181 agctgtctat aatcggacaa aagcaaaagc tgatccttta gtaacagaag gagctatctg
      241 ggcagataca ccaaaagcgg tagcagaaca aagtaatatt ctctttacaa tggtaggtta
      301 ccccaaagat gtagaagaaa tctactatgg acagtcgggg attttttctg cagatatctc
      361 aggacatatt ttagttgatt tgactacaag tacaccaagc ctggcagaaa aaattgcaaa
      421 aacagcaaaa gaaaaaggcg cagatgcgtt agatgcaccg gtatccggtg gtgacttagg
      481 agcaaagaat ggtacactga cgatcatggt cggtggagaa gaagccgttt atgatcaagt
      541 actgccttta ttcaaagaat ttggtacgac atttacattg catggttctg ctgggaaagg
      601 tcagcataca aaaatggcta atcagatcat gattgcaggt acgatgacag gaatgacgga
      661 aatgcttgtc tatgcacaaa aaaacggact tgatttaaag aaagtgattg agacgctttc
      721 aggtggaagt gcagctaact ggtctttaag caattattct cctcgtattt taaaagaaga
      781 ttacacacct ggtttttttg tcaaacattt tatcaaagat ttaaagattg cattagaaga
      841 agcagaaaaa atggatcttg tattgccagc aactacccaa gcattgaaac tatatgaaga
      901 gttggcagat aaaggttttg agaatgacgg aacccaagct ttgatcaaac tttggtggcc
      961 tgaaggaaaa ataccagaaa aaaaatcata aaaaacgagt ttttttagag aaaatcaatg
     1021 caagtaggct atgcttttga tacaatataa cagaaataag aaaaggagga cctccgatga
     1081 cacattcaca attagtcgca atcataaaac gactggaggc tatggtcgag tcagcagata
     1141 atgaactgca agttcgccgt tttgaaaaag aaggcgtaga gaagtgtaca gtaacttatg
     1201 ataaagcgac cgatacattt gaattaactg aaacagatac tcatcagcaa tacgaatttg
     1261 ataatatcga tatcgtagcg atggaaatct atgacttaat ccaataaatt ttaggataat
     1321 tgacatttga agttgaagat gcctaaaaga agccgagaag caactaattt agttgctttt
     1381 cagcttcttt tttgtttaac agttcaattt caaactgttc tttttctaat tttttgcggt
     1441 aactgatcaa gatttgtaca ggatcgattt cttcttttga caattgacgt ttcaaagata
     1501 aatgagcttc tttcttttca taatagaagt caggagaaac gtcaccagag gtaatcattg
     1561 tttctgcaag ttgaccataa caaacgaggt agtgcttttt ctgactctca tctgctgcta
     1621 atggcatgct gaaggtgagc gatggtgttc ttaaaaactg cttttgttcc tggatccgtc
     1681 taataaaata agtaactaat tcgatatctt tttgaagcat accggctttt tcgaaattca
     1741 actgatagat gaaatgttgt tgtctttttt tcaagtaagt cagtagcttt ttatttcggc
     1801 cttgaaacag cccttggagt tccttttttt tctgttcaaa agtcatttcc tgtaggtcag
     1861 gaagctggac tcgcaaagct aacaaactaa tttcagacaa ccaaggcatt tgataagttt
     1921 cttccattat acgcaaaata gacggcattt ttttatagag acgaaaaggc ccgtatgttc
     1981 gagccgtagg agtatctgtt aatacaaatc ccttattggt aatatgtaca taattataat
     2041 tactgaaata attcatttgt cggttataaa aaggacggta atgttggatc aattgacact
     2101 cgagcaataa agcatctaat tccgtgtcta ctacgacgaa atcaaaatct gttatatgat
     2161 gaatcatacg taatactttt tttgaatgct gtttattctg atgaaaatag ctgcttactc
     2221 tatttttgag ccgttttgct ttaccaacat agatgacctt accatctcgg tctttcataa
     2281 gatagacacc cggcagcatg gggagagttt tcattttttc ttttaacata ggatctcctt
     2341 ttacgctttg aataaaaaga ctataacaaa ttttgaaata atggacaagt tttagggaat
     2401 tctatagttc tgttcacaga aaatactttg ataatcaaaa tatgtgagat agatgatttt
     2461 tctaataaaa gaaattataa atgctgattt atcagttgtt tt
//
LOCUS       DAZKOK010000195         2487 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000195 DAZKOK010000000
VERSION     DAZKOK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.202"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..2340
                     /locus_tag="ACG90W_002812"
     CDS             187..2340
                     /locus_tag="ACG90W_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0479104.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          854 a          428 c          547 g          658 t
ORIGIN      
        1 aaataagttt attttttaat gaaaaaaata gttggatatg taactttaag tgattgctgt
       61 gaaacaaaaa gttgatttta tcttgaaatt aggtaaagaa tttcttgctc cgcccatttt
      121 ttctttttta ttaagaagtt tttgggtaca atgaagcatg aatgtaaata ggcggaagga
      181 taggaaatga tgaaagattt tatgaaaaaa atattaaata cttcttggtt agaaaaaata
      241 agaaaaggtg cacaagaacc taagaacaaa acgtttcgcc ggtcccatgt tccttttcgc
      301 ttaaattttc tttttttcat tatatttacg ttatttgttg cgttgattgc tagattgggt
      361 tatttgcaga tcgtgaacgg cgaagagatg gaggcgcgag tgaagtctac ttctacgatt
      421 acggtccaag aaagtactcc tcgcggaatg atctacgact ccacaggaaa agctttagtt
      481 acgaataaag cgaatcaggc catcacatat acgagaggaa cacaagttac ggctaaagat
      541 ttgcttgaaa tcgcaacgaa gctgaactcg ctgatcgatg ttccggtaga tgaaaatctg
      601 acagatagag ataaaaaaga tttttggctg gctgatccca aacatttgaa agaagccaca
      661 aaaagacttt cggcaaaaga aaagcaatta gggaacagcg aacaatatag tgcgatggtc
      721 aataaagtca cagctgatga gattaattta aacgaggagc aattaaaaat tgctaccatc
      781 tttaaacgga tgaatagtgc atatgaatta aacactgtct atatcaaaaa ctctggtgtt
      841 actgatcaag agttagctgt tgtggcagaa cgcgcttctg aacttccagg agtttcgact
      901 ggcacagact ggacacgaga atataatgca gcaagttcac tgaaaagtat tcttggcagt
      961 gtgacgactg aaaagcaagg acttccagca gatgaagcag agaaatacct agcaaaaggt
     1021 tattctcgga atgaccgtgt aggacaaagt tatttggaaa aacaatatga agatgtcctg
     1081 caaggaacca aaacccaata tgaagtttcc cttgataatg aaggaaatgt ttcgaatcaa
     1141 aaagaaatct tctctgggga aaaaggttct aatttaatgt tatctatgaa tgcagaattc
     1201 caatcaaaag tagaagaaat tttgaaaagg aattatcaaa cattgatcaa caacggcaaa
     1261 gcacagtatt cgcctggtgc ttatgcagtg gctatgaatc ctcagacagg agaagtgcta
     1321 gcaatgactg gtttcagtca tgaacaaggt tccaaagaaa tcaccgagaa tgctttaggc
     1381 acgattacta gcgcctttga tccagggtct gtagtcaagg ctggtacttt gacagcaggc
     1441 tgggcaagta acgcgatcag cggaaatcaa gttttgattg atgagccgat tcgtctgcaa
     1501 ggtgcttcag aaaaaagctc tgtctttaat cgaagcggac aagttgctct tgatgcagtc
     1561 aaagcatcgg aattgtcttc taatacgtat atgatcaaag tggcgttaaa gatgttagga
     1621 ctagactata cacctggaat gggactacct agtttagatg aagaagctaa agcttatcag
     1681 caattgagag actctttcaa agagtttggt cttggtacaa cgactgggat cgatttgccg
     1741 aatgaatctc ctgggatctc acgttctgtt gattatatga agaagttcaa tgcggataat
     1801 ggcaaagaat ggtatgctcc aggtaacttt accgatttgg catttggaca gtttgacaca
     1861 tatacaccaa tccaattagc tcaatatgct tcaacagtcg ctaatggcgg gaaacgagta
     1921 cagccgcacc tagtcaaagc gatctatggc aatgacgaaa atggaaactt gggtgaagtg
     1981 aagaaagaaa ttggcacaac agtagaaaat acggtcaata tttctgcaga aaatatggcg
     2041 atcctccgtg aaggattcca ccaagttgtc catggtaccg atccttacac aacagccaaa
     2101 ccattagcca gtgcgaaaat ggacctttcc gcaaaaacag ggactgcaga aacggttgcg
     2161 gaaggtcatc cagatattgc gacagtcaat agtaatatcg tggcatacgg accaacagac
     2221 aatccggaaa ttgccatcag tgttgtcttg ccgaacttat tagacgaaca agaccacata
     2281 aacttgacga ttgctaaaga aataatggac gcgtattatg atatgttcat ggcaaaataa
     2341 aaaagaggtc gtgaaaaagc cgcttagttt cgagtatgaa gaaaaatttt gtgaaaatag
     2401 cttctcacat ttttgcttaa atttgacttc ataccgagaa actggctttt gaacaccgtt
     2461 tactcggcaa aaaaggttgt gacaaaa
//
LOCUS       DAZKOK010000196         2463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000196 DAZKOK010000000
VERSION     DAZKOK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.203"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG90W_002813"
     CDS             <1..189
                     /locus_tag="ACG90W_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0479105.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="ACG90W_002814"
     CDS             complement(1055..1585)
                     /locus_tag="ACG90W_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0479106.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="ACG90W_002815"
     CDS             complement(1582..2199)
                     /locus_tag="ACG90W_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0479107.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          812 a          469 c          274 g          908 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taa
//
LOCUS       DAZKOK010000197         2458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000197 DAZKOK010000000
VERSION     DAZKOK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.204"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG90W_002816"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG90W_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG90W_002817"
     CDS             1320..1778
                     /locus_tag="ACG90W_002817"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0479108.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..2407
                     /locus_tag="ACG90W_002818"
     CDS             1937..2407
                     /locus_tag="ACG90W_002818"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0479109.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          716 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctttaactt aataaaaata aaaaaagatg aactatttct agttcatctt tttgcctt
//
LOCUS       DAZKOK010000198         2422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000198 DAZKOK010000000
VERSION     DAZKOK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.205"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACG90W_002819"
     CDS             <1..572
                     /locus_tag="ACG90W_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0479110.1"
                     /translation="LSFFITIFITLNDNGQSYADNFTIQGLLEYASRDSGYDGVGDLN
                     RITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSFGNLHYQYFHHIWLFI
                     EVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVFFFVYNTTLRGETTGP
                     LIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            569..1288
                     /locus_tag="ACG90W_002820"
     CDS             569..1288
                     /locus_tag="ACG90W_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0479111.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1303..2388
                     /locus_tag="ACG90W_002821"
     CDS             1303..2388
                     /locus_tag="ACG90W_002821"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0479112.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT          866 a          262 c          482 g          812 t
ORIGIN      
        1 tattatcctt ttttataaca atatttatta cattgaacga taatggacaa agctatgctg
       61 ataatttcac aattcaaggt cttttagaat atgcgtcaag agattctgga tatgatggtg
      121 ttggtgattt aaatcgaata actggtatct ctagagtagt agatgaaata tttaataata
      181 attttagatt agtcctaatg ggagtaggca ttggcaatgc agagtttacc aatttttttg
      241 tcagtgagtt ttataaatcg tttggtaatc ttcattatca gtattttcat catatttggt
      301 tatttataga agttggaata ttaggagttt cgatattcct attttttata ataaagacat
      361 ttttcaatgc aaaaagatct tttatgaagg ataaagatgc aagtcttgtt aaagttattg
      421 caattatgtc ggtattcttt tttgtgtata acacaacact taggggtgaa acaacggggc
      481 cactgattta tatgatttta gccatgcctg aagttgtgaa aaggaaagag cattattata
      541 gtgaaaaaaa tacaaaagga gagatattat gattcctaag aagatacatt actgttggtt
      601 cgggggaaat ccacttccga aaaaagatag aaagtgcata gaaagctgga aaaagtattg
      661 ccctgattat gagattattg aatggaatga atcaaattat gatttaaata agaaccgtta
      721 tatggccgat gcatataaag aacgtaaatg gggttttgtt ccagattatg caagatttga
      781 tattatttat aatcatggtg gattttattt agatacagat gttgagctaa ttaagtcgtt
      841 agatatactt cgtgaaaatc atgcctatat gggatttgaa ggggaaatct gggttaatgg
      901 cggtattggt tttggtgcag aagaaggaca ttctgttatt aaaggtttaa gggatattta
      961 cgattcatta agtttttatc aaaatgatgg tacattgaac ttgaaaccga gtccgtatta
     1021 tattacagat tatcttgaat ctattggatt ggttcgcgat aacagcgaac aaataataaa
     1081 tggcctgcat atctatccta cggattattt tgctgccaag gattatgata ctggaaaaat
     1141 ttataaaact agtaatacaa taagcattca tcaatataat gcatcttgga aaacacctaa
     1201 acaaaaattt gtttatagat taaaatcaac aattggacaa aaaaaattta acaggttact
     1261 tgcactgaaa aaatttttac acttataaag gagaaaagta ctttgaagaa aatttgtttc
     1321 attagtggtg taatttctcg ttctggggga actgaaagag tagggagtat tattgccaat
     1381 agcttgtctg atttaggtta tgatgtcact attttaagct tttggaatag tggagagcct
     1441 tattttgggt tagataagac gattaaggtt gattatatgt taaatcctaa aacggaggga
     1501 aaattatata gaacttattt atatccggtt tggaaattgc acaagtatat atgcaaaaat
     1561 gaatttgata tagtaattga tattgatact ttgttgtctt tgtatactac aagggcaatt
     1621 aaaggaacaa agtgcaaatt gatttcttgg gaacatttta attactggac aatggaaatg
     1681 ctgaaagaaa agaagagatt caaggctaaa aaattgataa aaaaatatgc aaagaggctt
     1741 gtggttctca cagaagaaga tcgtcaaaaa catattgatg aatatagttt atcagaagat
     1801 tttgttataa ctatgccaaa tccgtgtata tctaatatcg agtacgaata tcattatgat
     1861 aaaaagagat ttcttgcagt agggcgatta gtggaagaga aaaattttgc tgacatgata
     1921 agtgcttgga aaattgtaga aaaaaaagta gatgattggg atcttatcat tgtaggaaaa
     1981 ggtgaattag aaaaagatct tcgccagcag ataaaagatt taaatctgca aagagtaact
     2041 atagcagggc attcagacca tgtggaggaa tattataaat ctgcatcttg ttatgtgctt
     2101 agttctgttt atgaaggatt tcctatggtt atattagaag cacagtctta tggattacca
     2161 gttatttctt atgattgtaa aactggtccg agagatttgg tacatcataa ttttaatggg
     2221 atgttggttg aagataagaa tatagaccaa ttagctaaga gcatgataat gtttactaag
     2281 aatactgatt tagcaatgag aatgtcactg aacgcatata ataatgtaca aaaatttaat
     2341 ttgaaagaaa ttacaaaaca atgggttgct ttgattgaaa atgtatagtt ttttgtggtt
     2401 gtccaaacat gaaaattagc gt
//
LOCUS       DAZKOK010000199         2405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000199 DAZKOK010000000
VERSION     DAZKOK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.206"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..103)
                     /locus_tag="ACG90W_002822"
     CDS             complement(<1..103)
                     /locus_tag="ACG90W_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0479113.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(135..458)
                     /locus_tag="ACG90W_002823"
     CDS             complement(135..458)
                     /locus_tag="ACG90W_002823"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0479114.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            920..2302
                     /locus_tag="ACG90W_002824"
                     /pseudo
     CDS             920..2302
                     /locus_tag="ACG90W_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          718 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt
     2341 aaaaaactat tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc
     2401 aagaa
//
LOCUS       DAZKOK010000200         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000200 DAZKOK010000000
VERSION     DAZKOK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.207"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG90W_002825"
     CDS             316..570
                     /locus_tag="ACG90W_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0479115.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG90W_002826"
     CDS             571..888
                     /locus_tag="ACG90W_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0479116.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG90W_002827"
     CDS             1447..2382
                     /locus_tag="ACG90W_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479117.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKOK010000201         2367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000201 DAZKOK010000000
VERSION     DAZKOK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.208"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..515
                     /locus_tag="ACG90W_002828"
     CDS             186..515
                     /locus_tag="ACG90W_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0479118.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(802..1287)
                     /gene="vanZ-A"
                     /locus_tag="ACG90W_002829"
     CDS             complement(802..1287)
                     /gene="vanZ-A"
                     /locus_tag="ACG90W_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0479119.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1440..2351)
                     /locus_tag="ACG90W_002830"
     CDS             complement(1440..2351)
                     /locus_tag="ACG90W_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0479120.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          779 a          416 c          350 g          822 t
ORIGIN      
        1 gattaggaaa ttatgtagaa taataatatg tttttaactt ggaatcaaaa acgcacttgc
       61 aagtaagttt acttcaatac atgaaattgc ttataatagt gtattggaat taaggtagat
      121 tgacaaaaga gtgatcaaat aggtatactt cattcaaata atcgtttgaa tgtgaggtga
      181 gatgaatgaa aaaagtttgc caagtgactg tcgtacataa agaaaaagta aatggaataa
      241 aaaataaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa tgtttcagcg
      301 atttttctag aattaaaatt ttctatgctt tagaaaccta taaggaaatg tgcgtctgtg
      361 atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta cgttttttaa
      421 aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattac tctttagcga
      481 atgaagacat cctttccgct attcgtgttt tttaaagtta tcagaaaatc tatctatgaa
      541 atcttagttt gggggtaccg tcaggaaaat gcggatttac aacgttaagg aaatttatta
      601 aagagaccaa ttcctcagca ctaaaattga taattgggct ttctttgtta ctatagtgtt
      661 aggtaagctt tttactttat caccggtttt cccctcactt cacacctacg ggcgagtttc
      721 cccacatccc gttttccatc taatccaatt cattcaatat ctttatgtgt atgaaagatc
      781 agattgcttg atttagacat cttacacgta atttattctt aaatgggtac ggtaaacgag
      841 caataatacg agcaaaagta tacctacaaa aataataact ctgtctaatt ttttttgatt
      901 catatgctta ttgcttaaac catataattt cagtccaaga aagcctccaa cagtatttgt
      961 aattacatct gttatgtctg tcgctccaat agcgaagata aattgaatta tttcaaaagt
     1021 aagacttaaa accagtacaa aagcaaactt aggtaaaaat ccgatttctt taaaattgac
     1081 attcaaaagc aagccaaatg gaataaagat tataacatta tctatcatct ctctgaaatt
     1141 cccagtagca gtaaatggag tcaagttaag acttctttga tgataattaa atactgataa
     1201 aatattgtat tgtaatttga ataacactaa ccagattagt gtcactaaat ataaagctag
     1261 caatcctcta gataatattt ttcccaatat aatttcctct aattcttttg tttaagttgt
     1321 atcattagta tagcaatgtc ctagttaacg tgggttttaa attaaaaagc taacgttacc
     1381 ccaaaagtgc tagtacaata atgctgttca ttaatttcgt tggtttcatt ccgccatcct
     1441 tacctccttg aattagtatg tgttgatccg ggaaacactg tcacaattac accgtctata
     1501 ttgtttcctg atatctcata acgaagatta gtcggcacat gaatggtggt atttttagta
     1561 acaggataat aaaagatctc atatttttcc ccatttacac taacagaaat ggttttttct
     1621 tcttttaggt aatccatata ttcctcgaga acgaaattct tttctttcat aatcgcacta
     1681 tgtggtaaac caacatagcg aatatgccat ggttcatatt gaattcctgt taactctgtt
     1741 ttgtcctctg gataacgtaa aatgaacccg tatttccaag cattttcttc tatccacttt
     1801 ccttcagggg ctcgttccat tttcgtcaag cttgatccta catctagtga taaacctgaa
     1861 ttatgctcac tataacctgc tggtaaggca tactcagccc ccatttcttg gtaaagcaca
     1921 ctttgctcat caaagtctcg atagccacta ttaataataa aatgactaac gccacccttt
     1981 acagcatcat tgaccatctc tgaaaatttt tgtgctattt cttttgacat ataaatatta
     2041 ctatcaagca acccgtatcc atttattaat tcgtcatgtt tagataaatt cacgatatct
     2101 gacttcacac tttcttggcg aacaggatat ttactattga ttaatagcag atttccttga
     2161 taaacctgtt cttctgtaat tgttttctct tgggtatttt cagaagtccc actattttct
     2221 aaatgttctt tgggattttg atcataattt tgaaattcga ctttttcctg agaaaacagt
     2281 gcttcattaa cgtagtcata acctaagtat attaagaata acaataacaa taaaaaaaac
     2341 aacttcttca ttttcagtct cctcctt
//
LOCUS       DAZKOK010000202         2313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000202 DAZKOK010000000
VERSION     DAZKOK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.209"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1594
                     /locus_tag="ACG90W_002831"
     CDS             62..1594
                     /locus_tag="ACG90W_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0479121.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            1772..1993
                     /locus_tag="ACG90W_002832"
     CDS             1772..1993
                     /locus_tag="ACG90W_002832"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0479122.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          787 a          240 c          437 g          849 t
ORIGIN      
        1 attttagaaa ttatatagta ttaggtaaca ataaaaaata gaaagtttgg agccatatga
       61 tatgataatt aatcgtaaac aaaatgctaa aaggaatata attactggaa caatttttaa
      121 gttatatcag attatcattc ccttttttat gcgtacactt atgatttatc agatgggaat
      181 ggagtatgct ggattgaaca acctgttcac atctatattt caaatgttaa atttagcgga
      241 attgggaata ggtgctgcac taactgtaag tatgtataaa cctatagcag aagacaatag
      301 aaaagagatt tgtgctttac tgaacgcata taaaaagtac ttttcaatta ttggatggat
      361 agtgtttaca ttaggattgt tatgcattcc ttttttgaga tttttagtta aaggttcgat
      421 tcctggaaat ttaaatatga cagttttgta tttaatgtat ttgataaata ctgttttgtc
      481 gtattggtta ttttcatata gaaagagtct aatatatgca catcaaagaa atgatgtaat
      541 taacgtgtct atgctaatta cttttacatt ccaatttgta atgcaggcat atgttctgat
      601 tttcttaaaa aattactatt tgtatttatt ggcttctatt ttgggacaaa tagttcttaa
      661 cttaatgtgt gctgttgttt ctcaaaagat gtatcctgat tatactcctg ttggtgagtt
      721 agagaaagaa actattgacg atattataaa aaaagtaaaa ggattagtta caaacaaggt
      781 aggcggtaca atattacgat cttcagattc aattattatt tcatctttct taggtctttc
      841 agtacttgcg atttatcaga attattactt tattatttct gccataatga gtatatttgc
      901 aataatttat gaatcatgta tagcaggtat aggtaacagt cttattgtag aaagtcaaga
      961 aaaaaattat atagattttt gtgatattac aacaattata ggttggtttc tggcaattag
     1021 ttgtgcatgc tttattacat tatttcagcc gttcatgatt atctgggtgg gaagtgataa
     1081 tcttatgagt atttcatttg ttatatttat gatagttcat tttttcttat atgaaattga
     1141 tcagttaatt gggttataca aagatgctgc tggaatatgg tacgcagaca ggtttagacc
     1201 attaattaca gcaatgataa acattgtatt aaatatcatc ctcgttaatt ggttcggatt
     1261 atatggagtt ttggcggcaa ccattgtttc agtattgttg gttgatttac cctggttatt
     1321 atataatgtt ttccaaaata tatttaaatt tgaatcggca ggtaaatatg tttggaagct
     1381 cataagtttg tgtgtaatag cttttgttgc ttcttattta agttattttg cttgttcgta
     1441 tgttaatatc ggaaatttca aagtgataag tttagtgata aatttagtta tatcggttgt
     1501 catatcgatt ttagttattc ttctgtttaa tatccgtaat aagaagttta agtatatttt
     1561 gaaagttctt acgtcggtat tggtaaaaag atgagaagag acatcagaat gatatataaa
     1621 gtaactacaa tttttaggct gccgaatagg cgaacgatgt atagtttttg ttccaacgaa
     1681 gactcttgtt gaaattggag atgatatgaa ggagttacaa ttttgactca tgtttatgat
     1741 tgatcggttt taaaagtagt atggagaagc tttgggatca tcggagagga tcaaaatagg
     1801 taataatgtt tttataggaa tgaagtctag aatactaaag ggtgtacata taggaaataa
     1861 tgtagttata ggtgcaaata gtttagtaaa caaggacgta tcagacgatt gtgttgttgc
     1921 aggaaatcta gcaaaagtaa ttatgtccct agatgaatat tataaaaaga taaacaatat
     1981 gaagaatccg taaaacttgt gaaactatac cgagaataat atgggaaaaa tattgatgaa
     2041 aaagcattat atgagttttt agggctgttt acagatggag atgacaattt gtcgaagagt
     2101 tgggagcaat gcaaaattta gtaggggatt ataaacagac tgacaaggta tttaaagaac
     2161 atgcacaagt atttcataat attgaagaat ttttgaatac tgtgagatag gagttgctac
     2221 attgagaaaa gtatgtattt ttccaatgta aaagattacg agtttgaaaa cgacaccata
     2281 atattatttt ttaagaagat attatagagc tta
//
LOCUS       DAZKOK010000203         2281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000203 DAZKOK010000000
VERSION     DAZKOK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.210"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1950
                     /locus_tag="ACG90W_002833"
     CDS             88..1950
                     /locus_tag="ACG90W_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0479123.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1950..>2281
                     /locus_tag="ACG90W_002834"
     CDS             1950..>2281
                     /locus_tag="ACG90W_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0479124.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          818 a          340 c          450 g          673 t
ORIGIN      
        1 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
       61 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      121 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      181 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      241 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      301 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      361 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      421 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      481 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      541 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      601 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      661 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      721 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      781 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      841 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
      901 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
      961 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1021 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1081 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1141 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1201 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1261 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1321 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1381 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1441 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1501 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1561 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1621 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1681 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1741 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1801 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1861 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     1921 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     1981 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2041 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2101 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2161 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2221 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2281 a
//
LOCUS       DAZKOK010000204         2221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000204 DAZKOK010000000
VERSION     DAZKOK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.211"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..476
                     /locus_tag="ACG90W_002835"
     CDS             <1..476
                     /locus_tag="ACG90W_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479125.1"
                     /translation="SVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKS
                     TEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSN
                     LCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(537..902)
                     /locus_tag="ACG90W_002836"
     CDS             complement(537..902)
                     /locus_tag="ACG90W_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0479126.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(978..1223)
                     /locus_tag="ACG90W_002837"
     CDS             complement(978..1223)
                     /locus_tag="ACG90W_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0479127.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1293..1547)
                     /locus_tag="ACG90W_002838"
     CDS             complement(1293..1547)
                     /locus_tag="ACG90W_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479128.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1711..2034)
                     /locus_tag="ACG90W_002839"
     CDS             complement(1711..2034)
                     /locus_tag="ACG90W_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479129.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          836 a          368 c          386 g          631 t
ORIGIN      
        1 aaagcgttat tattttagtc tattttcagg gggggaacct tatgataaaa aaactatttt
       61 tactatcaat attaacgcta gcaatggcag gttgttctga ctctggctta gcaactgaac
      121 tgatcacaaa atcaactgaa gccacgacag aggagttaaa atgtggaaac gatcacagtg
      181 actacagagg cttaaatcga cgatgcatgg gaatattagg cagaagcaat tgtaagcatc
      241 gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa aatcaaaaga aagatcaaaa
      301 gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga tgcggtactt ttaaaccacg
      361 tcaggagatg ttcatttatg tctagggaag ttggcgatga accaaaacta gagtacgctt
      421 ttagttttgc acgtgaggat gaagataacc ctaaaactta cgacatttta gcttgatcta
      481 agatggttaa agctttttaa tgaagtaaga ggagatagaa ggcttttcta gttatattag
      541 gtaagataaa ttcctttttg ctccaaattt gctaaacatt ctgctagata aacttcatcg
      601 tgttccggat aaatcggcac gttgcgctct acagtaggta aatcaaaaaa tgcttcgcat
      661 gatttgccac gatacatagg atttttccac tctggtgctg ggctgtaacc acgtttttcc
      721 atttcatcca tgacaagctg atgatattgg aataatctat aaggagaata gtgaaaaaca
      781 taatccacag tagcatgttt ctttccccag ccatttcctc ttaatgcaca gcattcccga
      841 tgttgtccta aaagctgttg acgaggaagc tttgaaatca atttttcatg ccataatcgc
      901 atagaaatgt ctcctttata tatagattgt taaccttaga cgttaaatga aaaaaccagc
      961 accaaggctg gaaaaaatca cttcagtttg cttgctgttt ctgagcgacc aattaggtaa
     1021 tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt caatgatgct tggaatttct
     1081 tctccttctt cgtacttacg atatttgaat aaaggaatac ttaacgcgct agaaacagct
     1141 acttgagata aagatcggcc agttcttaag aaattcagac gacaacgtaa agtatccagt
     1201 aaaatagctt cttcgatact catttaatca cctttcatta tagtagaata gttataatga
     1261 ctatagaaag ctacacatgc aatcaatagt actcaatgtt ttttatttaa acgtatcaaa
     1321 taaagaatga cgatcaataa agacaaaacc agtcctgata attcttctat aaaaacacta
     1381 tcgggttcaa agtagttgta attaaaaact ttccacataa agtagatagc cggaagagtc
     1441 aaaatagcta aataaaaccg ttctttgttc caattcataa gataaacaat acttgcgacc
     1501 agtgcgaccg caggcaccca aaacaaaact ctcaccaacg taatcatgta acggactcct
     1561 caacgattca tataataaaa gataacatgt tattgcatac atcacaaatg aaatgctagg
     1621 aaaataaaag aagaatagat caatgaaaaa tgaaaaagca gaacagtttt ctgctgataa
     1681 gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa aaaatcaatg gattgatatc
     1741 taagtaattc aatacatata aaaagaggac gatcgcattc actaaaaagg aaagaatgat
     1801 gatcccatag cgaatggaaa catatttaac aaatactttt gtttgaatac gggcatatct
     1861 ttttccttta tagctgagca tcatgacaag aagcaaaagg gtaatcacac cttgaaaaat
     1921 cattccaata aacagaattc ctgaggtttg ataatttaat tggagaaaat aagctgctgc
     1981 cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata atcactccta acattgagaa
     2041 taaataaaag aataacattt tttctaaaat aagcaaataa aaattaaagt aacaaaatag
     2101 ttatgacaca agtaaaagaa ataagaataa caatattatt ttacttatat tttgaaactt
     2161 gtgagagttt gtcattcgtg ctatagttga aaagtaacaa aaataaattt tatttatgat
     2221 g
//
LOCUS       DAZKOK010000205         2219 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000205 DAZKOK010000000
VERSION     DAZKOK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.212"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG90W_002840"
     CDS             <1..878
                     /locus_tag="ACG90W_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0479130.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            1035..>2219
                     /locus_tag="ACG90W_002841"
     CDS             1035..>2219
                     /locus_tag="ACG90W_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0479131.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LEC"
BASE COUNT          693 a          459 c          439 g          628 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      961 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc taccaattca
     1021 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
     1081 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
     1141 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
     1201 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
     1261 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
     1321 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
     1381 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
     1441 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
     1501 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
     1561 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
     1621 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
     1681 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
     1741 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
     1801 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
     1861 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
     1921 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
     1981 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     2041 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     2101 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     2161 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgc
//
LOCUS       DAZKOK010000206         2160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000206 DAZKOK010000000
VERSION     DAZKOK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.213"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..945
                     /locus_tag="ACG90W_002842"
     CDS             70..945
                     /locus_tag="ACG90W_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0479132.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1090..1242)
                     /locus_tag="ACG90W_002843"
                     /pseudo
     CDS             complement(1090..1242)
                     /locus_tag="ACG90W_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1510..>2160)
                     /locus_tag="ACG90W_002844"
     CDS             complement(1510..>2160)
                     /locus_tag="ACG90W_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0479133.1"
                     /translation="TIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDP
                     FLIQNMQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKD
                     FAKLSVTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVV
                     QIPWTTPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          757 a          392 c          346 g          665 t
ORIGIN      
        1 tcagctactt ttttcttaaa cgtttcttta tactaaatat aaataagtgt ttcatgaaag
       61 gagaactaga tgaataattg gcttgagtat tttcctgaaa acgttttgga acgcggttac
      121 agctatcatc tgcacggttt tgtccgtcat ttgaactata caagcaagta cttgtccgca
      181 acagtttctg gcacggaaga ttataaggta gtgatcactt gggatgaaaa gaccaacatg
      241 acttgtgatt gtctatatgc catagaagga aaaaaatgca aacacatggc tgctgtctta
      301 ttcgcttacg aagaacgtcc aataaaaaaa tccaactact cactttccga actctcgtcc
      361 cttgtttcct ccgctagttc ctctcttgta cgggagcttt taacagagat tcttatagaa
      421 catccgcaat ttatcgaacg cttcaaagtt aagatgccat ttcatgcaat aaattattca
      481 gataaattaa caactatcat ccataaatac gatcatatta tcaagaaaaa caaaaacagg
      541 aaaacagcga agttcatcat ggagatgcga aagttcatcc aagaagcagt tgaatcgctc
      601 attcagcaaa atgcctatct tcccgcattc gaattgatca atgaagtgat tgctactctc
      661 gaaacattct attgggagcc tgaggatgaa cgaacccttc ttctgataga ggactgttat
      721 tatttatgga aagaattatt agcagaagca cctcatgcag aaaaaaggca aatgttctct
      781 tggttcgtct gccaagtcga tcatactgac gcctcttatt caaaacgcta ctccataaaa
      841 atcctgaaag aagactttcg ggaaaaagaa ttttctaacc aaaagaaaaa aatagataaa
      901 aaaactaaag aaacggtaaa gaaagacgat ttcaatgaaa aatagcaagt aaacgataac
      961 aaccatccta aatccacaag aaatacagca gaccttgctt taaaaacacg aatcatgcaa
     1021 aggatgaagt aaagaagact taacgcgtac ccaatattag tcatttgact attaggtacg
     1081 cgttttgttt tataagtcat gacaaatttc cttcgttgct gggtagattt tccggcattg
     1141 ttgatagatt ttatgatact tttctacatt ttgtaagaga ggttccactg catctgtata
     1201 ttggataaaa ttctgacaac aactttcagg tgtgtcgaac cactctaatc tttaaagata
     1261 aatattaaaa taacgattaa taatattaac agtctttatt tttcaccacc aaaaacctat
     1321 ctttgcatat actaaataga cgtatataca cagataggtt tgtcttgtga aaaataatga
     1381 ttatccttac caaaaaatgt ttgtttttct acatgttcgt acttatcaaa aaatatgatc
     1441 cttattgaaa ttactggcta tttatattcc aacgacttct tgtgttaatt tttgttatct
     1501 catagtaaat tatgtttcat tttcacttaa tttagttgtc acgtgttcgt caattcgtaa
     1561 aaacggtaaa tatataaaca cacttgcggc tataattaat atttgtaaaa cagctgctcg
     1621 ccagtctccg gctgttgcta aaaatcccga tataactggt ggcgtagtcc aaggaatttg
     1681 cactactaca tgattaatta atcccacagc agttgcaaaa tatgctactg tcaaagaaaa
     1741 aatcggtgca agcacaaagg gaataatcag actgatatta aatacaatag gcaatccaaa
     1801 aataataggt tcgcttatat tgaatatagc gggagttacc gataatttag caaagtcctt
     1861 ataatctttt ctacgactga agataaatat tgcaatcaat aaagcaatcg tatttcccga
     1921 tccacccatt accgcaaagg tatctttaaa ggccatattg ataatatgtg gaatttgctc
     1981 tttgtttgca taagctgcca tattttcttg catattctga atcaaaaacg gatctaacaa
     2041 tgaacctgat attactgctt gatggatacc aaatccaaaa aatagattag caatagatgt
     2101 aattaacaaa aatcctggta aaccagttgt aattttactt aatggctctg taactatcgt
//
LOCUS       DAZKOK010000207         2052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000207 DAZKOK010000000
VERSION     DAZKOK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.214"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG90W_002845"
     CDS             complement(<1..914)
                     /locus_tag="ACG90W_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0479134.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG90W_002846"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG90W_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0479135.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          683 a          368 c          399 g          602 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat ga
//
LOCUS       DAZKOK010000208         2032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000208 DAZKOK010000000
VERSION     DAZKOK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.215"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..566
                     /locus_tag="ACG90W_002847"
     CDS             <1..566
                     /locus_tag="ACG90W_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479136.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
     gene            complement(613..849)
                     /locus_tag="ACG90W_002848"
     CDS             complement(613..849)
                     /locus_tag="ACG90W_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479137.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            947..1126
                     /locus_tag="ACG90W_002849"
     CDS             947..1126
                     /locus_tag="ACG90W_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479138.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1257..1526
                     /locus_tag="ACG90W_002850"
     CDS             1257..1526
                     /locus_tag="ACG90W_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0479139.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1519..1776
                     /locus_tag="ACG90W_002851"
     CDS             1519..1776
                     /locus_tag="ACG90W_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0479140.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          741 a          290 c          414 g          587 t
ORIGIN      
        1 aagaacaatt gtatttggaa agtgaaacga gtcaaacgaa ggtcacagtc agccataatc
       61 gtttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacggag cttctacgac
      121 tagaaacaga aaaatacatc atgaatgttt acctagaaag gagtgagctg gtcgaaaact
      181 ttgtcagcta cacgatttgc aggaaacact attactcaga atcagaatat tattcaattt
      241 ttgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa gatgcgacga
      301 tagacgatta ttaccttgta tttactagat ttttgacgtg tggaacggaa tctatcgtgt
      361 taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa tatgtagaag
      421 aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta gacacctata
      481 cttacgacga tacagaagaa atccaaaatg aacttgaccg taaacgaatg ccatatcaac
      541 gatttgaaag gaaaaaaagg caataatcat ttataccgaa aagtgagaat gtgtgaaata
      601 aggacaaaaa acctagtaac taagggaatg gttcttattt cgcaccaatc gctccaaccg
      661 cccccgccgc ttaggtggtg cgaaataagc gggcaacacc cgataaccta agagaaagaa
      721 ggagatccat atgaataaaa tcattcctct attgttagtc ctatttgttg gcgaaatact
      781 tatgcgccat gctgcagctg tgcatttaaa cttcgtagta tttttggcaa tactggtatt
      841 ttgtttgatc ttttggcgct ttgtattagc ccttgtgagt ctactattca agatgattgc
      901 agccatattt ttcatagggt tatttcgatg aaaggagaga gtactaatgc caattgcaat
      961 aggaaataaa cggttacccg ttacgttgga tgaaaagaga caaaaagaat tgcagcaact
     1021 aaagcagaag tacggcaaaa gtgaatccag gattatgtgt attgcgttag atttattgat
     1081 tgcccaagaa aaagcaggat ttgaggtacc agcactcaaa aagtgacgtc actttttatc
     1141 ctaaaaacta aaagtgatag cacttttaat tataagaagt tagaatatta atcatttgct
     1201 taattgtaca atataatgta caattgtttt atagaaataa ataaggggtg aaaggaatgg
     1261 aagcagtagc ttattcaaat ttccgccaaa atttacgtag ttatatgaaa caagttaatg
     1321 aggatgctga aacacttatt gtaacaagta aagatgtaga agatacagtt gttgtattat
     1381 caaaaagaga ttatgattct atgcaagaaa cgttgagaac actttctaat aattacgtca
     1441 tggaaaaaat tcgtcgagga gatgaacaat tctccaaagg tgcatttaaa acacatgact
     1501 taatcgaggt tgaatctgat gattaaggct tggtctgatg atgcttggga tgattatctt
     1561 tattggcatg agcaaggaaa caaaagcaat ataaaaaaga ttaacaagtt aataaaagat
     1621 atcgatcgtt ccccctttgc tggattagga aaacctgagc cattaaagca tgatttatct
     1681 ggaaaatggt ccagaagaat tacagatgaa catagactga tatatagagt tgaaaatgaa
     1741 acgatattta tttattctgc aaaagatcac tattaaccaa tcggaagtaa ggaaagggtc
     1801 agaaacttaa aagtttttga tccttatttt atttacccta gtcatttaaa aagctaatat
     1861 agcttagtgt tgattgttat taatgaatgt gtttgttacg cgtattacgg atataaggtt
     1921 agtaaaatca tttctaaagt tgaggaaaag taaatataaa tggcttaaat ttcaacaatt
     1981 tgaagttgaa tagatatgtt ataatactat tgtagtgtgg gatgttagtt ac
//
LOCUS       DAZKOK010000209         2020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000209 DAZKOK010000000
VERSION     DAZKOK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.216"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..571
                     /locus_tag="ACG90W_002852"
     CDS             101..571
                     /locus_tag="ACG90W_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0479141.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="ACG90W_002853"
     CDS             793..1896
                     /locus_tag="ACG90W_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0479142.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          328 c          460 g          545 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac
//
LOCUS       DAZKOK010000210         2012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000210 DAZKOK010000000
VERSION     DAZKOK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.217"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG90W_002854"
     CDS             200..742
                     /locus_tag="ACG90W_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479143.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ACG90W_002855"
     CDS             898..1347
                     /locus_tag="ACG90W_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0479144.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2012)
                     /locus_tag="ACG90W_002856"
     CDS             complement(1377..>2012)
                     /locus_tag="ACG90W_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0479145.1"
                     /translation="RGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTD
                     LTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTD
                     RGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQL
                     RLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          729 a          314 c          383 g          586 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cg
//
LOCUS       DAZKOK010000211         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000211 DAZKOK010000000
VERSION     DAZKOK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.218"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG90W_002857"
     CDS             <1..378
                     /locus_tag="ACG90W_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0479146.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG90W_002858"
     CDS             426..1826
                     /locus_tag="ACG90W_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0479147.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKOK010000212         1991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000212 DAZKOK010000000
VERSION     DAZKOK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.219"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACG90W_002859"
     CDS             <1..580
                     /locus_tag="ACG90W_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0479148.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            650..1989
                     /locus_tag="ACG90W_002860"
     CDS             join(650..1091,1091..1989)
                     /locus_tag="ACG90W_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0479149.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          787 a          288 c          365 g          551 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cctctttgat
      601 gtgcacccca aaagttagac tagaaatcta attaaaaggg gtgctttttt tgcggaaata
      661 tacatttgtt tttaagaaaa aagtagtttc agactattta aataacgaag gcggctacaa
      721 atatcttgca cataaatatc aaataaatcg tacactggtt agacattggg taaggattta
      781 taactatcat ggttgggaag gcttggttgg aggtggcaaa agctacacta caaaatttaa
      841 acttgatgtt atagaatata tggaaacaaa tggtctttct atccaagaaa ctgctaaaaa
      901 atttaatatc ggttcaaata gaactctaag taaatggata gagcaatatg aagaaggcgg
      961 tgcttcttca cttgagagcc aaaaaagggg cagaaaaatt agtatgaatt ccaagctaaa
     1021 cattcctaaa aaacttaaag atgagtctct tgaagaagaa gttattcgtt taagagcaga
     1081 gaacgcatat ttaaaaaagt tagaaacctt gattcaagaa caagatttga gcaagaagaa
     1141 atcaaggtta aaacaatcct tcagctcaaa aaagaattta aattgaatct attactttca
     1201 attgcacaat tagcgaagtc aacttattat tactgggtaa aaaaattaga taagccagat
     1261 aaatatagca agataaagca agaaattaca gcgattgtaa aagaatcaag aaactcttat
     1321 ggttatcgta gagtcacttt agcgttaaag atgaaaggat atacaatcaa tcataaaaca
     1381 gttagaaaat taatgtccca aatgggactt acctgtcaaa tccgaataaa gcgatataaa
     1441 tcttataaag gaacagtagg aaaaattgcc aagaatgtgt taaaacgaaa tttttcagta
     1501 gatacaccca ataaaaaatg ggtgacagac gtcactgaat tcaagataaa aggaagaaaa
     1561 atctatctat ctcctatact tgatttattt aacggagaaa taattagtta tagtatatca
     1621 actagtccaa catataaact gattgaagaa atgcttcaac aagcaattaa aaaaaaggga
     1681 actgagggtt cactgattct acattcagat caaggatggc agtatcagat gccacaatat
     1741 caaaaaaaat taaaagagaa taacattatt caaagcatgt ctagaaaagg aaattgctta
     1801 gataattctg taatagaaaa tttttttggt gtgcttaaat cagagttttt ttatcgagaa
     1861 aaattccgat caattgagat atttcaaagt aaattaaatg agtatattag gtggtataat
     1921 aacaaaagga taaaactaaa gttaaatgga ctatctccag tagaatatcg gaaacagtcc
     1981 attaaatagt t
//
LOCUS       DAZKOK010000213         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000213 DAZKOK010000000
VERSION     DAZKOK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.220"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG90W_002861"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG90W_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG90W_002862"
     CDS             complement(728..1744)
                     /locus_tag="ACG90W_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0479150.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKOK010000214         1957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000214 DAZKOK010000000
VERSION     DAZKOK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.221"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG90W_002863"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG90W_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0479151.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG90W_002864"
     CDS             852..1040
                     /locus_tag="ACG90W_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0479152.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG90W_002865"
     CDS             1053..1610
                     /locus_tag="ACG90W_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0479153.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG90W_002866"
     CDS             1642..1884
                     /locus_tag="ACG90W_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0479154.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          305 c          404 g          533 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttctt
//
LOCUS       DAZKOK010000215         1938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000215 DAZKOK010000000
VERSION     DAZKOK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.222"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACG90W_002867"
     CDS             complement(<1..252)
                     /locus_tag="ACG90W_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479155.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSD"
     gene            complement(254..1681)
                     /locus_tag="ACG90W_002868"
     CDS             complement(254..1681)
                     /locus_tag="ACG90W_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0479156.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            complement(1674..>1938)
                     /locus_tag="ACG90W_002869"
     CDS             complement(1674..>1938)
                     /locus_tag="ACG90W_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFQ0479157.1"
                     /translation="SFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWF
                     DERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          741 a          319 c          222 g          656 t
ORIGIN      
        1 atccgagaaa aattgattat ggttagttaa caagttaatt ttacgtttaa aggaagaacc
       61 gattttccgg tgattcacaa aaacaacatc attaaatagc cctaattctt ttatacgctc
      121 actatatctt ctagcgtttt caaattcaga ataaataaat aaatctcctt ttgattctcg
      181 gtaaaaattt tgataaacat ttaatatatt aatcaaatgt aaaggagtcc aagcaatgaa
      241 agctgtcttc atattaattc tcctttttta aatacaagta agaaatgctt cccaaataat
      301 accagaaaac aattgaaatg acatcgcgct tattgaaaag tacatgagcc tccaccattt
      361 catctataaa aatagatagt ataaaaatta aaacaatacc aagaaacgca taattcactt
      421 tacttctaaa aaatgttttg agtaaactaa agatatttaa cacatagaaa gcatagatac
      481 atataaaagc aattatacca cttgatacaa gtacttgaag aaatccatta tgcatgtttc
      541 catgtgctct ttttagttca ttcacatctg tagtatcaag atctactttt ttcaaaaatt
      601 ttgcatcgct attcccatag aaatcggtct ctccataacc aaataacaaa gttggtctta
      661 taactttaag cgcagctttc caaatcgtaa aacgaccagc tgaaatttcg gagttatcac
      721 tagaatgctg taatattact tctttttctg gaagtggagt tttatctgat tcatttgact
      781 ctataacttc tacttttcta tcaatcgata caggaattgt tgctaaaaaa tccttagcat
      841 aattagtacc aaaagaaaaa tataccaata gaactaataa tatgcttacc atccatttga
      901 tttttacagg tagtacttct ctaatgctct ttctaaattt tgaattggca aacaacaaaa
      961 gaaataatac taaaaaagaa caaattacta cctgggcacc tctagacgaa gaaagtgtaa
     1021 tataaaatat ttgtacgata aaattcatca tataaaaaac acgaatcgtt tttgttattt
     1081 ttgaaattaa tacactaatt aaacttaaaa taatagaagt atatccaaac atagctccaa
     1141 catttggact tgtatagaca ccaaataacc gatgttcaat aaaaccttgt cttgcaagta
     1201 atcctggcgt tcctgttggg acatgataag aaatcaaaaa aataaattga tagatagata
     1261 ttagcgcagc taaaaaaatt atgataatga aaacattatt aaaatacacc atttttttct
     1321 ttttttcctc ttcactttgt gaaaagtctg caggaaaaat tactatcaaa gtaatcagca
     1381 aatatccaaa attataaata gatggaatta aaaaattttc tctgttaatg attatagtta
     1441 agataaaact aatcattgct ccaatcagta gccaagaaaa acgcatttta aaaaaatttt
     1501 tctttataaa aacctcatac aaaactataa ttccacccca agcaaaagct attttcatcg
     1561 ggatattagt tacattaaaa gtactaggca taaaataaag cagacaaatg attaaattta
     1621 ttactcgaaa cgcatctcga cttaataaac tcttctctaa aacaggtact attctattca
     1681 tctatatttc caactttcaa aatttcttta tcaattctat ttaatagttt atcagagtaa
     1741 tccgatttaa cttttcttac aaataatttt tcactttctt ttatttgatc tatatccctt
     1801 tcatcaaacc atttgttcat ggaaggatca ataatatgaa tatttgctaa tttcgaagtt
     1861 tgtaaagtag tttcttcaat atttttcaat tcaagtggta aatctggtcc tccaataaca
     1921 tttttatttt tgaacgag
//
LOCUS       DAZKOK010000216         1907 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000216 DAZKOK010000000
VERSION     DAZKOK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.224"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1693)
                     /locus_tag="ACG90W_002870"
     CDS             complement(227..1693)
                     /locus_tag="ACG90W_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0479158.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          604 a          375 c          321 g          607 t
ORIGIN      
        1 aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat aaatggtgtt
       61 caatcagctg accttcagca ttagctcaaa aaacggcaaa atatgaggag ctatttttgt
      121 ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat tcacggtgtt
      181 ccagaagtag cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
      241 ctgtttctgg ttcagtaatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
      301 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
      361 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
      421 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
      481 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
      541 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
      601 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
      661 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
      721 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
      781 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
      841 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggga
      901 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
      961 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
     1021 tgacataagg ggatattttt tccgcccctc caactgcaaa tactagcatc gaaacggttg
     1081 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
     1141 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
     1201 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
     1261 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
     1321 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccacca gcaatcagaa
     1381 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
     1441 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
     1501 tatccttgaa ggctgatcct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
     1561 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
     1621 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
     1681 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
     1741 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
     1801 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
     1861 gaccttattg aatttcaagc tattttgaat tttctgcaaa attaaaa
//
LOCUS       DAZKOK010000217         1876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000217 DAZKOK010000000
VERSION     DAZKOK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.225"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..1135)
                     /locus_tag="ACG90W_002871"
     CDS             complement(95..1135)
                     /locus_tag="ACG90W_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0479159.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          647 a          281 c          231 g          717 t
ORIGIN      
        1 ttattttgta atattcgtaa tataattaga ataataaaaa atttttaatt ttaatgttac
       61 tccatgaagc aacaatcact ttgtcagtaa agtttcactc taaccagtct ttcgtaatag
      121 tgcttgggga gcttgcataa ttcaaacgtt tttcacttga tactttagca gctattggtt
      181 tttcgataaa ccaatttttt acatagacaa aaatcaggtt ctggatattt tcgacttttt
      241 cggtttttaa tttttgatag gcttttaaca gtgtcgtgta taattcttgt tctgcattga
      301 cataataact atcgagttct tcaaaaacta tcgaaatatc actttctatt tcagcttttt
      361 tctttgcatt atgaatagtc tggacggttt tttgagcttc actgtaactt gaagaaaatg
      421 tttttatcaa atttaatact cgttcaggga caaacgtagc aatgctgtcg tttgtcataa
      481 ttgtctcaaa attgtctaac aacaattgat cttgtaggcg gtctttttca gtgtctatat
      541 tgtgtctatt agtgtctaat atattattat taagttctgg tatgattttc ataccctctg
      601 atttatcaac gttttggcac ggtatgattt tcataccctc ttgagcccca agggattcag
      661 cgtctgattt ttcgtcgagg gtgggtatga ttttcatacc ctctgattta tcaacgtttt
      721 ccctattctc tctatcaaat ttttcgagta agtagatatc attttctgag acttttaatt
      781 ctgctagata tagtctatta ggattatttt tccctaactg tttgttaaat cccatctgct
      841 tttgtaataa taaaccaaag gattccaact cttttttgat tgctatcgct ttttgttttt
      901 ggcagtttaa tactctacat agttcatttg tcgtataagt aaaataaaca ttaccttctt
      961 catcgatttg ccgtttgctg attgcgatct ctaatcttgc ttttaaaagc atataggcaa
     1021 tcttagttga atctttcatt tttttgtatt cgtcagaaac aataaagact tttggaaatt
     1081 gataaaataa ttcgttgtaa actctatccg ccttaaaaaa tttaaaatca gtcattgtag
     1141 atcctctact ttcacgttgt tttatttatg taaaattacc tactcttttc gaaaatagca
     1201 aagataaata aaaaatgcaa acgcattaaa actgtttttt aattaaatac tacctaaaat
     1261 cagaaataaa atgaattatg tactaccttt aaagtcatta agaactactt taaataaata
     1321 ttatttataa ttaaatacta ccagaaaaac gtatgaataa aatttagttt taaaattaaa
     1381 gataattaag tactaccagt gtcaaaacaa aaaataaatt aagaactacc ttatgcatat
     1441 aaaacatact accattacta gtaatatata ctaccttatt tcagtagtat atattatatt
     1501 attcttataa gacaaggctt taggctagaa aacaatagat tatatactac taaaaaatac
     1561 aataagtact accaatgtca gatatatata ataattatgt actaccatta aaattaactt
     1621 atttaataag atactactgt gttaatctga aaaaaattac tttaaagtag ttacatacta
     1681 cctgttaatt tagatactac cattaatact taaattagat actacctata tttatattaa
     1741 gtactaccgt tctaagaata agataataaa aaaatactac caaattgcaa ttaaatacta
     1801 cttaaaatgt aaaaaataag aactacccaa attaggtagt tcttaataag agaatatcag
     1861 ataaatttgt acaagt
//
LOCUS       DAZKOK010000218         1856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000218 DAZKOK010000000
VERSION     DAZKOK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.226"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..562)
                     /locus_tag="ACG90W_002872"
     CDS             complement(53..562)
                     /locus_tag="ACG90W_002872"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0479160.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(674..1672)
                     /locus_tag="ACG90W_002873"
     CDS             complement(674..1672)
                     /locus_tag="ACG90W_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0479161.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT          562 a          353 c          332 g          609 t
ORIGIN      
        1 caaaaaagaa agaactcgtc taaatagacg ttttctttct ttttttgttg aattattgag
       61 taaggcgact tcttttttct ggttctttgt gtgctaatcg actggcagca gcagcggcga
      121 tcgctccgac gatatcatct aagaaggtat ggacatgaac gccatcgtgg ctgtttagtt
      181 cttttaaaat accaggtttc accttgtcga tataaccata attcgtaaag ccgatcgtac
      241 cgtaaacatt cacgattgac aaggctagga tctcatcgat accgtaaagg ccttcgtcat
      301 cttcgataat atgttgcaac ggactcatca attttttctt ttctgctaat atatctaatt
      361 ggattccagt gataatggcg ttatgtactt cacgctttgt tagcacgctg ttgacacttt
      421 cagtacaatc cgccaaagtc aaatcagaaa tatagtcttt ttgtaaaaac atgacaagtt
      481 gtgcgatatc ttctacagtt acgccacgtt ccttcaatag ttctcttgcc ttttcttcta
      541 aagtctcagt tttgacgacc atattattgc tcccccttta ttttttcttt ctctatttca
      601 tgttaacgga acatgaaaaa aatgcaagaa actgctttcg ggtttaactt gaaatgcaaa
      661 gagatataaa aaatcaacta ggacgaagat ttttatcgta taagcttgtg ctgtgaccag
      721 gctgagttcg ttcctttgga ttaatatagt gtaaagcgtt gttcactgca gtaggtgctt
      781 caccgagccc agtagcaatc agtttcactt ttccttcata ggtacagatg tcaccagctg
      841 cataaacgcc tggaatagaa gtagacatat ctgattttac agtaatggca tttctggtac
      901 tatcaagtcc ccaagaagaa agatgttcta aattagaaga gaatccataa ttgacgatca
      961 gagaatcaat catcaaatca atcgtctcgt cacttttcac tttttttaac cggatatctg
     1021 tcagttcccc attattgcct gataatccat cgatgagata gggagtaagc aagttgacag
     1081 atgaagattt caagcgtgat acactatgtt cgtgcgcacg aaattccggg cgacgatgaa
     1141 tcaagtaaac ttcgcttgca ataggctcta acatcaacgt ccaatcgata gcagaatcac
     1201 cacctccagc aatagcgact ttttttccag catacttcat caaatcattc acgaaataat
     1261 ccaatccgtg gttttcaaat gattcagcat tatctaaatt caattttctt ggctggaatg
     1321 aaccatttcc taaagctaaa atgactgctt tggaataatg gatgccttta tttgtagtaa
     1381 tctcgataac ttcgtcttca cgagtaagcg agagaacttc ttcttttaaa tggaatgtat
     1441 ggttaaaagt agttaattgt ttttctaatt gatcgattaa ttcacttgct tttattgctg
     1501 gatacccagg aatatcgtaa atatattttt cgggatacaa agttgctaat tgaccaccaa
     1561 gttgaggtag actgtcaatg atttttgttt tagcttggcg cattcctgcg taaaaagcgg
     1621 caaacatccc tacaggacct gcaccgatga ttgtaatgtc atagatttcc ataaaaattc
     1681 ctcccttttc acactatagc agaatttaat tgagaatgct tctcaaaaat gtttgaattc
     1741 gaaatcgaaa cggtaagtta tagaatgtat ctagtgatga aaaataaaag aaaacaaatc
     1801 tagtattttt acgagaggtt aggtattatg ttattattat tatgaagtat gcaaat
//
LOCUS       DAZKOK010000219         1752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000219 DAZKOK010000000
VERSION     DAZKOK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.227"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG90W_002874"
     CDS             134..307
                     /locus_tag="ACG90W_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479162.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="ACG90W_002875"
     CDS             947..1420
                     /locus_tag="ACG90W_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0479163.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="ACG90W_002876"
     CDS             1429..1656
                     /locus_tag="ACG90W_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479164.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          588 a          272 c          260 g          632 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tt
//
LOCUS       DAZKOK010000220         1722 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000220 DAZKOK010000000
VERSION     DAZKOK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.228"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..>1722
                     /locus_tag="ACG90W_002877"
     CDS             275..>1722
                     /locus_tag="ACG90W_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479165.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIRE"
BASE COUNT          627 a          242 c          309 g          544 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aa
//
LOCUS       DAZKOK010000221         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000221 DAZKOK010000000
VERSION     DAZKOK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.229"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG90W_002878"
     CDS             131..505
                     /locus_tag="ACG90W_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0479166.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG90W_002879"
     CDS             complement(619..1248)
                     /locus_tag="ACG90W_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0479167.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG90W_002880"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG90W_002880"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKOK010000222         1662 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000222 DAZKOK010000000
VERSION     DAZKOK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.230"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            220..>1662
                     /locus_tag="ACG90W_002881"
     CDS             220..>1662
                     /locus_tag="ACG90W_002881"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFQ0479168.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT          563 a          315 c          327 g          457 t
ORIGIN      
        1 gttattcgaa ccttatttta agttacttat tatttaatgt atcgaaaaag gtgaaatagg
       61 tttgttataa taagaattaa gtaagacatg aattgtctaa gcaaatgtag cttatccatt
      121 tcattaaaat acatatggtg aaaaaacaag ttcaaatagt aactaatttt ttaaaaaact
      181 gatttataaa aaatacaaca cgaatgagga aaataaccaa tgacagaaca aatcgaaaaa
      241 aattcaaaaa gcttatatca ggcactgtgg aattccgcag atatcctgcg ttcaaaaatg
      301 gacgctaatg aatacaagag ctatttacta ggcttggtct tttataaata cttatccgac
      361 aacatgctgc ggtatgtctc cgttctattg gaggaagaaa cggacgacct aaataaagca
      421 caagaccttt acgttgaagc atttaaagat gcagatataa aggatgatct cttgtatgaa
      481 ctaaaagatg agttttcata taccatcgca cccgcattga cctttaccgc gcaagtggca
      541 gccattcatg atggatcttt tcaattagaa gacctggtac aaggtttccg tgacattgaa
      601 caatccagcg aaaactttga gaacttattt gaagacattg atctgtattc taaaaaactg
      661 gggtcaaccc cacaaaaaca aaataaaacc atcgcagatg ttatgaaaga attagaaggg
      721 ttaaacatgg cggggcatgc tggcgatgtc ttaggcgatg cctatgagta cttaattggc
      781 caattcgcat ctgaatccgg taaaaaagcc ggtgaattct ataccccaca accggtcgca
      841 aaattgatga cacaaattgt tttacaagga aaagaagata aaaaaggctt ttctgtttac
      901 gacgcgacca tgggttcggg ctcgttactt ttgaacgcta aaaaatattc ccatcaaccg
      961 ggaaccattt cttatttcgg acaagagttg aatacgtcta cctataattt ggcgcgtatg
     1021 aacatgattt tgcatggggt tcccattgcc aaccaacact tgcataatgc ggataccttg
     1081 gatcaagact ggccaacaga agagccaacg aacttcgatg gggtgctcat gaacccaccg
     1141 tattctgcaa aatggtcggc tgataaaggc ttcttagatg acccacgctt ctctgcctat
     1201 ggcgtattag cccctaaatc aaaagcggac tttgctttct tattacatgg ctactatcat
     1261 ttgaaagata cgggcgtcat ggccattgtt ttgccacatg gggtgctctt tagagggggc
     1321 gcagaaggga agatacgcaa agccttgtta gaaaatggtg ccattgacac cgtcattggc
     1381 ttacctgcta acatcttctt taatacgtcc attccaacga ccgtaattat cttgaagaaa
     1441 gaccgtgcga aaaaagatgt gctctttatt gatgcgtcac aagattttga aaaaataaaa
     1501 acgcaaaata cgctacgaga ttaccatatt gacgctattt tagaggccta taaaacccgt
     1561 acaattaaag acaagtatgc ttatgtcgca gaatttgatg aaattgtgga gaatgattac
     1621 aacttaaaca ttccccgtta cgtggatacc tttgaagaag aa
//
LOCUS       DAZKOK010000223         1636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000223 DAZKOK010000000
VERSION     DAZKOK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.231"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1502)
                     /locus_tag="ACG90W_002882"
     CDS             complement(183..1502)
                     /locus_tag="ACG90W_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0479169.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DAZKOK010000224         1628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000224 DAZKOK010000000
VERSION     DAZKOK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.232"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..504
                     /locus_tag="ACG90W_002883"
     CDS             22..504
                     /locus_tag="ACG90W_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0479170.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            541..1551
                     /locus_tag="ACG90W_002884"
     CDS             541..1551
                     /locus_tag="ACG90W_002884"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0479171.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          588 a          254 c          350 g          436 t
ORIGIN      
        1 aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca cgaaaatata
       61 tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt ggtaataaag
      121 aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga taaaaaagat
      181 aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc attagggaag
      241 atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca aacgggacaa
      301 aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat tattgatgaa
      361 gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca aacgatggtc
      421 atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat atttttgatg
      481 ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag gtgaagcaac
      541 atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc accatcctca
      601 ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat ggaagaaaaa
      661 tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa atcaggtcca
      721 gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat ccaaatgatc
      781 tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga ttttgatgct
      841 ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact tgctgtcaca
      901 ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag aggagagtac
      961 ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg agaatcgatt
     1021 ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc atcatcttct
     1081 gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc agtaaggatg
     1141 aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg tactccttat
     1201 ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt gtggtttcta
     1261 gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat gaaactggca
     1321 ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga ggatttgtta
     1381 gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact accgatcttg
     1441 tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg tgcctatgca
     1501 gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta aaaaattttt
     1561 tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag tgaaagaagc
     1621 tgacaaaa
//
LOCUS       DAZKOK010000225         1569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000225 DAZKOK010000000
VERSION     DAZKOK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.233"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..641
                     /locus_tag="ACG90W_002885"
     CDS             <1..641
                     /locus_tag="ACG90W_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0479172.1"
                     /translation="ADNIGISEVLLSYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEI
                     NRDSEIFSLSKDDKIVMGKRIREVRKNREETLEKFGKNFTIYTGKNVISRWEKGINIP
                     DIEKLMNIAYLGKVTVPYLMYGEDYKNILPKDERVSDFKKINSFSMGLRMRKIRKDYY
                     LEREEFGKLFSPSISKWSIDRYENGRDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT          586 a          167 c          320 g          496 t
ORIGIN      
        1 tagctgataa tataggaatt agtgaagtat tactcagtta tgtgttaaat agaaagagaa
       61 atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct attaagtaat gaaaaagaaa
      121 ttttgaagga gattaatcgt gatagtgaga tatttagttt atcaaaagac gataaaattg
      181 ttatgggaaa aagaatacga gaagtccgaa agaaccggga agagacgctt gaaaaatttg
      241 gtaaaaactt taccatctat acaggcaaaa atgttataag tagatgggaa aaaggtatta
      301 atattccaga tattgaaaaa ctaatgaata ttgcctatct tggtaaggtc actgttcctt
      361 accttatgta cggagaagat tataagaata tacttccaaa agatgaaaga gttagcgatt
      421 ttaaaaagat aaattctttt agtatgggat taaggatgag gaaaattagg aaagattatt
      481 atctagaaag ggaggagttt ggaaagcttt tttctccttc aatttcaaaa tggtccatag
      541 ataggtatga gaatggtaga gatataccca atactaatcg aattatccaa tatgcttata
      601 taggaaatct ttctttagaa tttttgattt acgggatata aaaatagttt acgtcgatac
      661 ttattaagtt atcgacgttt tttattatag aaaaaattta taaaaggagg tttcttgata
      721 tgataagaaa caaaaagggt ttgttgaaag ttgctttact ggcagctttt ttctaagtaa
      781 ctttttgaca agctcaatgg caacacaagc ggaagaagta atacaagaac taactgttat
      841 gaataacagc tagataaaat gggctatgta tgttatccaa caatttagta atcactagaa
      901 gcctagcgaa agttcgcttg ataagttata gatgagtatt gatttgtagt gtttccaaat
      961 caaaagggta cagagccaca tgaaatatgt tgtcctctgc agcgtcagga tgggaatcca
     1021 taccatggag attcggagat ctgtgaggga atacttacgt ggttaatatg tcagttgtgt
     1081 tggacgtagt aatggtcata gtgggtagat tgactgaatt cttatatgag aaaatgaaac
     1141 caacaagaag atagggttat ttatacagaa ttggggatct tttctggaaa agtaatcgga
     1201 taatgcgaga ttcgtacggt cttttttaaa tttctctaaa aaaaacggca tcgaatgggt
     1261 tgggtgattg ataagcaatg aatcatagac tgatatagaa taaaaaatga ttctatttat
     1321 ctttttacat ctatcgtttt ttcaagtagc cgcaaattat atcagcattc aacaattttt
     1381 gtatacaagt tctagtaaaa ggatctgaag aaaaagttgg ttgttgtgaa gataaaaaaa
     1441 gaatcaggtt ttactgagtt tttcttcaaa atttgtgtta ttaggttagg acaaagataa
     1501 gaagaagtat gaagatttaa aaaaggaatg aggaaaagat agtaatttcc gattattgga
     1561 gttttttta
//
LOCUS       DAZKOK010000226         1556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000226 DAZKOK010000000
VERSION     DAZKOK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.235"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            311..643
                     /locus_tag="ACG90W_002886"
     CDS             311..643
                     /locus_tag="ACG90W_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0479173.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            553..747
                     /locus_tag="ACG90W_002887"
     CDS             553..747
                     /locus_tag="ACG90W_002887"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0479174.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            786..932
                     /locus_tag="ACG90W_002888"
     CDS             786..932
                     /locus_tag="ACG90W_002888"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479175.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1370..>1556
                     /locus_tag="ACG90W_002889"
     CDS             1370..>1556
                     /locus_tag="ACG90W_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0479176.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT          552 a          280 c          277 g          447 t
ORIGIN      
        1 cctaaaatta ctttatttac gtatgaagtg ggcaggaggt cgaatccaga actgggctat
       61 ggtacttcaa cgaggacttc tcctctagaa ttgaaaagca tgcgatttac cttccgtaac
      121 gacggtagat ttattcaact atgtgtccaa tcaaaatttg tctgatttca aaaatagtca
      181 agagaatttt ggttgttaaa aactcttatc tatcaacttc cacagtttag ttgacaaacc
      241 ctgatactaa gttgggctat gatacacttt tcttgactat cagttacgtt accggaagga
      301 atgaactcta ttgagaaaaa catatgatcg taaatttaaa ctaaagattt cacaggatat
      361 ccttgagaaa aaagtaacta ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac
      421 aatttcccga tgggtttcag aatatcgtcg atacgggaaa aatgcttttg ctggacaagg
      481 aaaaagatta cctgataaag cagatttcta cattcttgaa caggaaaata aacgactaaa
      541 agaggaagac gagtgttcta cgaacacaaa gatcgatacg gatctccagg gatcactcaa
      601 aaactttacg atgaaggaat cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa
      661 atgaatttat gcacaagagg atttcatcga cgcaaatctt ctcatggtag agaaaaaaca
      721 atcaaagaaa tggtaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg
      781 tttgggtgat ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct
      841 atattgactt agcaactcgt attctacgtt actatatggt agattcacac atgaaaaaag
      901 agattgtcgt ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg
      961 atagaggtag ccagcatcgg tcttttattt atcaagagct gttgaacgac caccacatca
     1021 cacattcagt gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc
     1081 gatcaatcaa atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg
     1141 tgttaatcca agattattta gaagactact acccaaacaa aagaatccat acaaaattta
     1201 tgatgactcc ttgacaattt gaagcgaaga aaataaggga tctataatat ctagtgttgg
     1261 tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttaa
     1321 aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc
     1381 agagtttacc aaagagttac tagatattct agacctaaat cttatctttc atgaagatgc
     1441 ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt tttgatggca cattgaccta
     1501 tcaaccggag gaatgctttc atcgccacta tcaaaatgaa caaacgatca ttaaat
//
LOCUS       DAZKOK010000227         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000227 DAZKOK010000000
VERSION     DAZKOK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.236"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG90W_002890"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG90W_002890"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKOK010000228         1532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000228 DAZKOK010000000
VERSION     DAZKOK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.237"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..1483
                     /locus_tag="ACG90W_002891"
     CDS             17..1483
                     /locus_tag="ACG90W_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0479177.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          419 a          289 c          325 g          499 t
ORIGIN      
        1 aattggggta tagattatgg aaaaacagca agacaagggc ttcttcgggc agccgaaggg
       61 attgtcaact ttgttcttca ctgagatgtg ggaacgattt agttattacg gtatgcgtgc
      121 attacttatc tattatatgt acgatacagt tgccaacggg ggacttggat taccacgagc
      181 aacagcatta gctatcatgt cgatctacgg atctttgatc tacatgtcta gtatcgtcgg
      241 cggatgggta tctgaccgag tgttaggagc aaaacgcact gtattcttcg gtggagtttt
      301 cattatgctt ggccacatcg tcttggcttt accatttggt gtaagtacat tgtttgtttc
      361 gatggcattg attattatcg gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg
      421 acatctttat tcaaagactg acttgcgaag agatgcagga ttttcgattt tttacatggg
      481 tgtcaactta ggggcattat tagcaccact aatcgttggt acaatcggtc aagaatataa
      541 ctatcattta gggttctctt tagctgctat tgggatgttc tttggattat tacaatacat
      601 catccaagga cgcaagacat tagacggagt gggtacaaca ccgccgaatc ctttgacaac
      661 tgaagaacgt aaaggattca tccgtaactt gatcatcgca cttgttgtta tcgctgcagt
      721 attcggcggg gcacaactaa ctggtcatct gacaattgac ttttttgtca atacaatcag
      781 tgtattgggg attatcttac cgttgtatta ctttatcaaa atgctgactt caaaagatgt
      841 aactgctgaa gaaaaaccaa gagtatgggc ttatatccca ttattccttg cagcaatcat
      901 tttctggtca ttacaagaac aaggttcatc tatcttagct ttatttgcta gtgaacgtac
      961 acaatctacg ctatttggtg taacgatcca agcaagctgg tatcaatcat tgaatccaat
     1021 tttcatcttt attctgacac cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca
     1081 accatctaca gtagtgaaat tctctcttgg acttttattt gcaggattat cgtacttgct
     1141 attgatgttg ccgggtatgc tttatggtac aagtggaaaa gtaagtccat tgtggttgtt
     1201 gggaagtttc ttcatcgtgg ttattgctga attgtgtctt tcaccagttg gtttgtctgt
     1261 gacaacaaaa ttagcaccac gagcatttga atcacaaaca atcgctattt ggttcttagc
     1321 tgatgcttct tcacaagcaa tcaatgcaca aattgcacgc ttctacacac cgggaacaga
     1381 atctgcttac ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct tgctatttac
     1441 tgtgaaagaa ccaatcaaaa aactaatggg ctctattcat taatcaatga acataaaaac
     1501 aggaattgct tatttgcagc agttcctgtt tt
//
LOCUS       DAZKOK010000229         1523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000229 DAZKOK010000000
VERSION     DAZKOK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.238"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..157)
                     /locus_tag="ACG90W_002892"
     CDS             complement(<1..157)
                     /locus_tag="ACG90W_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0479178.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDN"
     gene            851..1237
                     /locus_tag="ACG90W_002893"
     CDS             851..1237
                     /locus_tag="ACG90W_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0479179.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1331..1522
                     /locus_tag="ACG90W_002894"
     CDS             1331..1522
                     /locus_tag="ACG90W_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479180.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          564 a          234 c          208 g          517 t
ORIGIN      
        1 tattatcagt tagtaacttt ttataatcat aaaaaaaata agtagttcta tctgagcgat
       61 actcttttat aatactgaat tcggaattaa aaaccacgat aggtaacttt aaacaaacat
      121 gaagagactt taataagact tctttatctg aaaacacttt tgtttctctc tttcctttct
      181 acatcattaa atagtatttt atacgttatc taaaatacta ttcctctgtc aaaataagct
      241 aacttacttc attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt
      301 acattttatc accgagaact catcattttt catcatgatg aatactattt acatatatct
      361 agttcaaaga caataattat tatgatccgt tattatctaa gcactataca gataatagga
      421 atttttcgtt tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt
      481 gtctattcga actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat
      541 ctatacacga ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa
      601 aaagctcaag acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa
      661 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
      721 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
      781 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
      841 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
      901 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
      961 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
     1021 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
     1081 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
     1141 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
     1201 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
     1261 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
     1321 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
     1381 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
     1441 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc
     1501 gaaattcgaa attaaagaat aac
//
LOCUS       DAZKOK010000230         1516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000230 DAZKOK010000000
VERSION     DAZKOK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.239"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..862
                     /locus_tag="ACG90W_002895"
     CDS             2..862
                     /locus_tag="ACG90W_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0479181.1"
                     /translation="MGMIQVRQGNKNTEVMEATIVENGLPYDLSECKVYFESVVGGKY
                     PVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAV
                     SKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWR
                     EGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLY
                     QKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQN
                     VFTLEKVGVI"
     gene            862..1482
                     /locus_tag="ACG90W_002896"
     CDS             862..1482
                     /locus_tag="ACG90W_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479182.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          540 a          217 c          348 g          411 t
ORIGIN      
        1 cgtggggatg attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat
       61 agttgaaaat ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt
      121 aggtgggaaa tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat
      181 taactatatt tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat
      241 tatatttaaa ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa
      301 tgctgtttca aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat
      361 tgcggatatg acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga
      421 aaggaaagaa gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag
      481 agaaggacaa gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac
      541 gattgatcca ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg
      601 agttcgtcat gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa
      661 attgcgagca acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca
      721 ggatgatctc ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat
      781 tgaagaagga gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct
      841 tgagaaagtt ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa
      901 acgggtgtac aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa
      961 gaaataatag caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc
     1021 attactaaag aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa
     1081 gaagatgacg gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt
     1141 gactacgtgt tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta
     1201 ttgacgattg atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg
     1261 ctggagttaa aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact
     1321 attgatgaag atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag
     1381 tcatatgtag atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa
     1441 agaattccaa attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta
     1501 aaaataaaac aaagca
//
LOCUS       DAZKOK010000231         1479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000231 DAZKOK010000000
VERSION     DAZKOK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.240"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG90W_002897"
     CDS             <1..828
                     /locus_tag="ACG90W_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0479183.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="ACG90W_002898"
     CDS             828..1445
                     /locus_tag="ACG90W_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479184.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          544 a          192 c          311 g          432 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcg
//
LOCUS       DAZKOK010000232         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000232 DAZKOK010000000
VERSION     DAZKOK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.241"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG90W_002899"
     CDS             complement(150..1337)
                     /locus_tag="ACG90W_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0479185.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKOK010000233         1414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000233 DAZKOK010000000
VERSION     DAZKOK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.242"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(178..1197)
                     /locus_tag="ACG90W_002900"
     CDS             complement(178..1197)
                     /locus_tag="ACG90W_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0479186.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1194..>1414)
                     /locus_tag="ACG90W_002901"
     CDS             complement(1194..>1414)
                     /locus_tag="ACG90W_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0479187.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          403 a          292 c          254 g          465 t
ORIGIN      
        1 acataaagcc ataaacaatc aaccagtaga atgtgtggca acaaacctgt tatcgcatag
       61 cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac tattttatcg
      121 aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga gctattttta
      181 tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac cgcctttagt
      241 gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat tagggaagtt
      301 acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag cttgatttac
      361 atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag tattgtaatc
      421 cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta catgcaaatt
      481 tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat ctgtcagtgt
      541 aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag aagttgttgg
      601 attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac ttacgcccat
      661 ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg ttacccataa
      721 atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa taccatattt
      781 cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa acgttgcttt
      841 atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat acgaaccagc
      901 accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt ctcccacaat
      961 ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat tgcgtcctgt
     1021 tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta cttgtgagct
     1081 accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc cgttaattgg
     1141 caataaaaaa agagccgtta acaggctcat cgcagtaata gtaattttct ttttcatttg
     1201 tttcctcctt cttcgctttc agccgagaac attttgtagg ttcgatttga tacacccaac
     1261 acactcccta aaaacgcgcc aaaaccagta atgatgacaa cacagatatc tgtgtactgc
     1321 caattgagcg ctttaccaac taaccccacg aaagtagcta gtgcgggaat aattaccagt
     1381 gcgaaccatt ttagtacttc gaacgtttta ttat
//
LOCUS       DAZKOK010000234         1381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000234 DAZKOK010000000
VERSION     DAZKOK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.243"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..483)
                     /locus_tag="ACG90W_002902"
     CDS             complement(112..483)
                     /locus_tag="ACG90W_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479188.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(485..886)
                     /locus_tag="ACG90W_002903"
     CDS             complement(485..886)
                     /locus_tag="ACG90W_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479189.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(900..1307)
                     /locus_tag="ACG90W_002904"
     CDS             complement(900..1307)
                     /locus_tag="ACG90W_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479190.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          440 a          243 c          177 g          521 t
ORIGIN      
        1 ggacgcacgt agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
       61 aagatacaaa gattaaataa aaaaataaca aacaaactcg cttgaaattc attaggtgag
      121 tttgtttatt attttttctg cttctcgatt catcagcgcc atatctgttt tcgtaatggt
      181 ttcattagga atatagtcag cacattttct tttctctaac attctatacc cactattatg
      241 tattcctaag gtattcaatt ccttaactgt tgaaaacaaa ttccaaacgg tttggtggct
      301 tccttttccg ctacttccat tataaataat atcataattt tctttcaaat actcatcaga
      361 ttttttaaaa gcactgtagt atgctcttga aacagcagtt cttctataag attctttaga
      421 attatattta tcatctgatt caatttgttt ggaaatagat aaatattcat taaagtcaaa
      481 cattttattc ccccaaagtt gctactacag atttccccat atatttatag tcagataata
      541 cattatcgag agtatccaaa acatcgtaat ctgaggaaaa caattcagat gttgttttga
      601 taacaaagaa atacaagttt aaatcttcat catattttat attgatttca gcttttcttt
      661 ttaaaaccgt atttatcaca tcaaaaattt tgccatcatt acttgaaatt gtgttaatct
      721 cattttttgt agtaattaca agttgattat catttttagt tttcggagta gaacttgcat
      781 ccacaacaac tggagatgct agaattaccg atgcgcaagc aaaaatcgca gcaaaattta
      841 ttaagctacc actccgatgt gtatttgatg gtactatatc aagcatccgt atcgtctcct
      901 taattttttt ccactggttt gttgaccttg atatcaaaga tttgccttcc aatctcagtt
      961 ccattaaatt taacaacagc ataatgctct cctgcttctt caaccaagac atttctggcg
     1021 tttatattaa agtttatatc gttaacttga ggaggcaagc ttaccgatac ttcacccgta
     1081 gaaaaaattt gtttttcatt cttgtttaaa acttcaattt ctactatttt tatcttttct
     1141 gtatcctcta tctcagacac cacagctgtt acagaaaatg ataaagcagt aggaattaaa
     1201 ggcgcattaa tagaaagagc tggattaatt actttattaa taacattagt gccaaattgc
     1261 tgactttcta catcttctga aattattaat tgtactctat aagacattga tttctcctaa
     1321 tttttacatt tatttaataa atttatgttt cacttttgct agtcaattta gtttaacaat
     1381 a
//
LOCUS       DAZKOK010000235         1374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000235 DAZKOK010000000
VERSION     DAZKOK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.244"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..1340
                     /locus_tag="ACG90W_002905"
     CDS             39..1340
                     /locus_tag="ACG90W_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0479191.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          275 c          253 g          398 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DAZKOK010000236         1373 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000236 DAZKOK010000000
VERSION     DAZKOK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.245"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..359
                     /locus_tag="ACG90W_002906"
     CDS             <1..359
                     /locus_tag="ACG90W_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479192.1"
                     /translation="RDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCRTCI
                     QKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQSIIG
                     VKTLAYLSGMGRNRRF"
     gene            677..1267
                     /locus_tag="ACG90W_002907"
     CDS             677..1267
                     /locus_tag="ACG90W_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0479193.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT          506 a          152 c          268 g          447 t
ORIGIN      
        1 ttagagataa tgagttcgat tgtaatattg aattagttaa aaaaattgaa ggcgatttaa
       61 gctatatatt agaaaatgag atatttgtaa acaaaacaag gagacttttg ttgggacgct
      121 gtcgtacttg tattcaaaag gcagttagat atgctagttt taataaagaa tttgatttaa
      181 actcctattt gtctaagata cttatgaacg atgagttgat tgcctgtatt cactcatacc
      241 catataaaca aaatccaatt aaacaacgaa tatttaacta ttttttacta aaccaatcca
      301 ttattggggt taaaacatta gcatatttaa gcggaatggg tagaaatagg agattttaat
      361 cataaaaaac tgtactatag tgaatttttg gagatttata ttccagttat atattaatga
      421 ttgtgatact tgtactattg taaaaattcg ttaggtttaa ataactggaa atatcggaaa
      481 atgggcatta tacatcacgg acatcccttc ttttggaaaa aatgttacga tttttataag
      541 taatattgta tccggtggaa tcaacgatga agataacata tccatcgttg tagattcagt
      601 agcattgaat gatgctcttg ttgcaagaaa tccgaataaa attgttaagt ataaattgaa
      661 ttaataggag atatgaatgg aaattagcag taaagaatat ttaaaagcgg ttcttcgtat
      721 ggaaaagaat ctatatatag aatctaaagg acaacacaca aaattactaa aaataatgaa
      781 atcacatccg gactacaacg cttggcggta tactcgtcga atgagaatta catcttatta
      841 ttattcaaag agaaagaaaa atatcattta tgcgattatg tatattgtgt cttgcagaag
      901 aatgaatagt ttgggaagaa aatgtggtat agagtgtggt gaaaatgtat ttgatgaagg
      961 tgtaagattt taccatacac aaggaattgt tattaatggt aacgccataa taggaaaaaa
     1021 ttgttactta tatgggaata actgtattgg aaatgatgga atcgatccta agtgtcctgt
     1081 aattggaaat aatgtacgtg tatgcgtggg tgcaaagatt attggtaatg taaaaatagc
     1141 taacaatgta gttgttgcgg caggagctat tgttatcaaa gattgtctag agaatggtgc
     1201 aattcttgca ggtgtaccgg caaaaataat agggtatgtt aatttaacaa aagtattttt
     1261 agaataattt ttagcgaaag aaggtgaata acgatgcgta tttcaataaa aagatggtca
     1321 ggagcagatt ggacattgtt cacgttggca ttttttgcaa tctccatagt tct
//
LOCUS       DAZKOK010000237         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000237 DAZKOK010000000
VERSION     DAZKOK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.246"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG90W_002908"
     CDS             82..>1364
                     /locus_tag="ACG90W_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0479194.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZKOK010000238         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000238 DAZKOK010000000
VERSION     DAZKOK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.247"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG90W_002909"
     CDS             <1..459
                     /locus_tag="ACG90W_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479195.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG90W_002910"
     CDS             473..904
                     /locus_tag="ACG90W_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479196.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACG90W_002911"
     CDS             906..1043
                     /locus_tag="ACG90W_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0479197.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            1081..>1352
                     /locus_tag="ACG90W_002912"
     CDS             1081..>1352
                     /locus_tag="ACG90W_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479198.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          506 a          196 c          278 g          372 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
     1201 aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa atcagatcga
     1261 ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca tacagaaaaa
     1321 ttggtggaac acacgcaaca aattaaaact tt
//
LOCUS       DAZKOK010000239         1347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000239 DAZKOK010000000
VERSION     DAZKOK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.248"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..624
                     /locus_tag="ACG90W_002913"
     CDS             <1..624
                     /locus_tag="ACG90W_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0479199.1"
                     /translation="LAQLLIMSMNNYVFPDYYNDAQGIVLMNFINPILVLIVVSPLTL
                     FLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWAN
                     ITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGD
                     VLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            634..>1347
                     /locus_tag="ACG90W_002914"
     CDS             634..>1347
                     /locus_tag="ACG90W_002914"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0479200.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVL"
BASE COUNT          479 a          146 c          255 g          467 t
ORIGIN      
        1 cttgctcaat tattaattat gagtatgaat aattatgtat ttcctgatta ctataatgat
       61 gcacagggaa tagttttaat gaattttatc aatcctattt tggttttaat agttgtttcg
      121 cctttaactt tgtttttaag taaaaaatat ggaaaaaaag aactggcatc agtaggaatg
      181 ttcttttcag caatagtata tttgatctta tttttgataa aagctagtaa tatgtatgta
      241 ttcttaattc ttagttcggt tggctatatg ggtttgggtg tgtttaatac tgttatttgg
      301 gcaaatatta ctgatgttat tgatgatcaa gaagtaaagt ctgatcaaag agaagatggg
      361 accgtatatg cggtatattc ttttgctaga aaaataggtc aagctctagc tggaggcgtc
      421 ggaggttgga ctctttctat tataggctat gactctttag caaatgtaca aacgggagat
      481 gttttagaaa agttatataa cgcttcaaca ttaattccat caatttgttt tctgattgta
      541 ggattaattt tatttttcct ttatccatta tctaaagata aagtcttaga aaattgcgag
      601 cttttaaaac aaaaacataa ataggagaga aatatgaata attggatatt ttgtattgat
      661 tcagatggct gtgttatgga tacgatgact tataaacaca atctcttttt cggaccaatt
      721 gctgcagaga tttttgatgt tcatcataga gaaaaattcc tacaggattg gaataatatt
      781 aatttatatt cagagactcg tggaataaac agatttttag gtttagttaa aacattagaa
      841 aaaaatgatg taacatcgat taatcgatta aagcactggg tatctctaac aaaagaattg
      901 tctaatccat ctttgaagag ggaaatagaa tattctcagt cagaggattt attaaaagct
      961 ttaaaatggt cggaagaagt caatcaacag attagtcaag caaaaggatt agataaaccc
     1021 tttttaagtg ctagagatac tataaaagct ctgaaaaaat atgggaagat aattattgta
     1081 agctcagcta ataaggaagc tgttcaagaa gaatgggaaa gacatgaatt attatcatta
     1141 gttgatgaac tgtgttgtca agataaagga aaaaaagaag atattattcg aagtgtaatt
     1201 gaaaatggtt gtgatttgga taagatatta atgattggtg attctcctgg tgatctagaa
     1261 gctgcaaata aaaataaagt tttcttttat cctattttgg tcaataaaga aaaagaatca
     1321 tgggaaaact taagaactga tgtttta
//
LOCUS       DAZKOK010000240         1339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000240 DAZKOK010000000
VERSION     DAZKOK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.249"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG90W_002915"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG90W_002915"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0479201.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          458 a          180 c          223 g          478 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatatttt
//
LOCUS       DAZKOK010000241         1326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000241 DAZKOK010000000
VERSION     DAZKOK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.250"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1082
                     /locus_tag="ACG90W_002916"
     CDS             129..1082
                     /locus_tag="ACG90W_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0479202.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1054..>1326
                     /gene="prgO"
                     /locus_tag="ACG90W_002917"
     CDS             1054..>1326
                     /gene="prgO"
                     /locus_tag="ACG90W_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0479203.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
BASE COUNT          479 a          199 c          246 g          402 t
ORIGIN      
        1 tttagtatat ccactatatt ccttataaag tctgaattct tatcaatttg gagtatatta
       61 gatatatcga atatattaag tatatgaagt atactaaata tatttagtat aggaaattgg
      121 agggaactat ggaaaactta cggactgcat ttaaaacatc agataaacta ggaaaagttg
      181 taatgcttac aggtagaaaa ggcggcattg gtaagactac agacaatgat ttattagcga
      241 ttgtttctag tcaacttttt gaaaaagacg tgttgctaat tgattatgac caacaaagaa
      301 atactacttc caacattgga tcaacgtatc aaattactag ctttgatcga tccatgtctg
      361 cagcaatcaa aaaaggggac tgggtttcag gtattactca agtaagtcca catttatata
      421 ttatggctgg ctctcctgga tctgaagaac ttaatgaata cttgtccgaa aaatatcctg
      481 atcgtcgtaa gagatcattg gccttcatta aacctttaga agaattgaga aaaaatttcg
      541 attatatctt tatcgactgc ccaccatcaa cagataatgt ggttcgtgca ttcttaactg
      601 ctgcagatta tattattgca atgcaagaat taaaacggta cgcaatggaa ggaacagaag
      661 attttattaa taaagtttta gttccgattg tcacgaattt tgaagagtct catcttcaaa
      721 tcattggaat tttgcctgtt cttttctcag tgcgacgttc ctctcaacac gcaaactatc
      781 aaaaaacaat tgataagtat ggtgaatcaa acatttttaa aagcattgtt aaaggctcgg
      841 atcggttaga aatgtatggt gagaatggta tccaattaaa tgattatgta gatcgaagat
      901 ggtgggctat ttttgcagac atctttacag aattagaaga aagaattgaa tactatgaac
      961 aacatggtga tattgaagga tttgattata cacctaaata tgctgactca atggccaatc
     1021 atatcctacc tcttggaaag gagattaaac taaatggcat tattaccaac gaacaaagat
     1081 aagaagaaaa ttggtgctga agatatttca attaaagatg atattaatgt cgagttaggg
     1141 gaacgaaagt acactgcgaa agaaagaaaa ccagttcaag tagatcctcc cgtactaaaa
     1201 accattcggg atcttagtta tgcaaaagat attcctatgt atgaaatagt caaacttgca
     1261 atggacgcct atgttgatac tttaaacgac gaggaacgga ttatatataa tagacgatca
     1321 aaagat
//
LOCUS       DAZKOK010000242         1296 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000242 DAZKOK010000000
VERSION     DAZKOK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.251"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG90W_002918"
     CDS             85..624
                     /locus_tag="ACG90W_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479204.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          488 a          167 c          205 g          436 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt acttta
//
LOCUS       DAZKOK010000243         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000243 DAZKOK010000000
VERSION     DAZKOK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.252"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG90W_002919"
     CDS             <1..1244
                     /locus_tag="ACG90W_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0479205.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKOK010000244         1285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000244 DAZKOK010000000
VERSION     DAZKOK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.253"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..284)
                     /locus_tag="ACG90W_002920"
                     /pseudo
     CDS             complement(111..284)
                     /locus_tag="ACG90W_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG90W_002921"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG90W_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          454 a          216 c          175 g          440 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacct
//
LOCUS       DAZKOK010000245         1242 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000245 DAZKOK010000000
VERSION     DAZKOK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.255"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            677..763
                     /locus_tag="ACG90W_002922"
     CDS             677..763
                     /locus_tag="ACG90W_002922"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0479206.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          372 a          256 c          208 g          406 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa
     1021 gctaaaaaac tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca
     1081 tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt
     1141 tgcattaatt tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat
     1201 ttttcgtctg ttaaatcgaa cttgattgct tgaattgctt gc
//
LOCUS       DAZKOK010000246         1239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000246 DAZKOK010000000
VERSION     DAZKOK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.256"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1239
                     /locus_tag="ACG90W_002923"
     CDS             <1..>1239
                     /locus_tag="ACG90W_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479207.1"
                     /translation="RTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVS
                     ISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYF
                     WYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLF
                     ILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMII
                     TLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYT
                     TSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVT
                     VPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISIL
                     TYCLVVYSSFKRFAPNKLKD"
BASE COUNT          431 a          191 c          222 g          395 t
ORIGIN      
        1 atcgaacagt tgatatctat tatcgatccg caattgcaga agcaatcgct cttacgatta
       61 tcccagctgt tttatattat gcttatggat tgatattcaa tagagaaaaa aaagtcatcg
      121 ctttatctgt cagtataagt ctacttgtgt atactcatgt cttaactaca ctattgtgta
      181 gtgcattgat tgcttgtatg attatagttc gtttgttttt aagcaagcaa ataaaaaacg
      241 atataaaatt tgtggtcgtg aatttagtaa aggctgtttt tttgacttgt gcattaacta
      301 gctatttttg gtatcctatg ttgcagcaga tgaattatca agaaatacac agtcccttca
      361 aaacagattt gcagctagaa gcactttcta tttcagatag tatcattcaa gcgttaaata
      421 acgatattac aacatacact atggggttat taggattggt tgcgttgatt tttcctttat
      481 tcatactgaa agaaatggag aaaaaagaaa aaatcatcta tgttggcgta gtttctacat
      541 gggtatgcgt aactaattta tttccatggt ttttgtttca aaaaacaccg ataagcttac
      601 tccagtttcc ttggcgtatc ttaggtatcc aaatcttttt ttcttcaatg atcataacgc
      661 ttgtttttat gaaaaagact atgaataaaa aatattcatg gttaatcatt ggcgctactg
      721 tggtgtttct actcgtcacg tctatagcag caaaagaaaa ttacgcaaaa gttatccaat
      781 caaaacacgg tcatattgtt atcaatgaag aaacactccc acgttataca acatcagata
      841 tgggaggatt atacgattac gccccaacag aagcgataaa agaaaggcct cacttagaaa
      901 aacatgaagt aaaagtagga gaatcgtggg aaaaagggaa gtataaagct gcagataata
      961 acattactta cacagtggca agcaacaaaa agcaaaaagt gacagtacca gtatatgctt
     1021 atttaggtac acaggtaaaa gtaaacggta aagtcgtaaa tgactcttat aaaaaacatg
     1081 gcttagtgaa tgtggatgta aatgctggag aaaataagat agaaattaca acaaaatata
     1141 ctccaactgc tctcatggct ttatggatca gtattctgac ttattgttta gtggtgtatt
     1201 caagttttaa acgatttgca cctaataagt tgaaagatt
//
LOCUS       DAZKOK010000247         1233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000247 DAZKOK010000000
VERSION     DAZKOK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.257"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..223)
                     /locus_tag="ACG90W_002924"
     CDS             complement(<1..223)
                     /locus_tag="ACG90W_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479208.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSN"
     gene            complement(240..>1233)
                     /locus_tag="ACG90W_002925"
     CDS             complement(240..>1233)
                     /locus_tag="ACG90W_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0479209.1"
                     /translation="LYMFKTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNS
                     LQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQN
                     YDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFE
                     KLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEH
                     LSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKIS
                     TAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          426 a          210 c          140 g          457 t
ORIGIN      
        1 aattgctacc attaatatga ctgatagatt ctctagcttc ttcacataaa ctttttactt
       61 tattaattac tactttgtca tattcattta acacactatt attaccatta cttagacgct
      121 cagattctac gaaacaactc aaatcagaaa tttcccaaaa aaattttttg ttaaaatcca
      181 atttcctcaa ctcctccaaa ctacgtaaat gatatagtct cataataaaa taaaacctct
      241 cagctaatgt tgttgaaatt gtccactaaa cctaattgat attgcttagg agatacacct
      301 gcgtactttt tgaatactct cgaaaagtgt gaagaatctg aaaatcctaa acctaaagaa
      361 acttcactga tagtatggtt tgatttcaac attattttag ccgtagaaat ttttcgctta
      421 gagatataat tataaatcgt aatatgcatt tcattcttaa agctttctct taatttagtt
      481 tcacttacag aaaaataatt agctatttcc tttacagtaa ttcttttata gatatttaag
      541 ccgatgaatt gaacgacaga agaaattaag ggagaaagat gctctatctt agtttttccg
      601 atttccttag tataataaac aatcccagaa tccctcactt ttaagacctc aggtaacgat
      661 acagctaatt cgttttcttg tatcaaagca tctctacttt gatacgcttc tattatatcc
      721 attcctaatg ttatagacgt ttgtgctaac ttttcaaaaa caatgataga ataatttttt
      781 tcagaccgaa tcgtatctcc actaacactt ggaattattc cattactcag attaaaaacc
      841 atgttttcta aatttcgaat atctccattt ttaacatatt ccagtatttt attttcataa
      901 agaaacagat atatttctgg atcgtaattt tgtgataaca gattatctat tttgatttgc
      961 tcgatagttt tacttagatt ttctgtatat tcaattaacg gttcatggaa tagatcttct
     1021 atttttccag taaaaaaata atgaactaaa atcagaatat ctcgaatgtc tcctaaagaa
     1081 tacaaagtca aattttgcaa tgaattatag agtcgttctc tatcagccat tgcaacattc
     1141 aaattgttca tttttagttt aaaaaaaact ttatcaatat tatttgctct aaatggacca
     1201 aataagtaat atctggtttt aaacatgtat aga
//
LOCUS       DAZKOK010000248         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000248 DAZKOK010000000
VERSION     DAZKOK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.258"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG90W_002926"
     CDS             414..947
                     /locus_tag="ACG90W_002926"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0479210.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKOK010000249         1214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000249 DAZKOK010000000
VERSION     DAZKOK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.259"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1026
                     /locus_tag="ACG90W_002927"
     CDS             <1..1026
                     /locus_tag="ACG90W_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0479211.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="ACG90W_002928"
     CDS             complement(1080..>1214)
                     /locus_tag="ACG90W_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0479212.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DAZKOK010000250         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000250 DAZKOK010000000
VERSION     DAZKOK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.260"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG90W_002929"
     CDS             <1..1076
                     /locus_tag="ACG90W_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0479213.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZKOK010000251         1175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000251 DAZKOK010000000
VERSION     DAZKOK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.261"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG90W_002930"
     CDS             <1..969
                     /locus_tag="ACG90W_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0479214.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          396 a          209 c          234 g          336 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagtt
//
LOCUS       DAZKOK010000252         1170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000252 DAZKOK010000000
VERSION     DAZKOK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.262"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..480
                     /locus_tag="ACG90W_002931"
                     /pseudo
     CDS             166..480
                     /locus_tag="ACG90W_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(785..>1170)
                     /locus_tag="ACG90W_002932"
     CDS             complement(785..>1170)
                     /locus_tag="ACG90W_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0479215.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          421 a          163 c          212 g          374 t
ORIGIN      
        1 taatcttctt tatagagaat tgggctattt ttaatggcaa agaggggaag aagatgtttc
       61 aaaacaaagg acaacgctat gttgtaaaag gagtaaagga atcttactgg aatcaaaaat
      121 ctttattgag aaaattatct ttgaatttaa gcacaaagaa atgaaggtac actagatagc
      181 aatgaagata tctttgtcat taaacgtcgc tattttacac aagtaagtga tgatttgatt
      241 catgtctttt ccaatgagtg gtttgtttct gagaaaaaat tacatgcttc taatctgcaa
      301 tatatgccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
      361 gaaggatata aagccaaaca tccagaagcg aaaccattta aatacccaca agaaatgaag
      421 cgagcatggc gtaaaatgtt agatgatgag ctgattcccc ttgaaaatga attgaggtaa
      481 acgagaagaa ttcaataaac tggatgataa aaatataata tgactataaa ttggttacag
      541 cttcaaccta taagttgtaa taaattagcc taaaagtaat ttattaatga tgaggtgaat
      601 ttgattggta caacaagata tatcacttag agagggagaa gcagcactac aaactacttt
      661 tcaataagta ggaattatag aaattttact caaaaaatct tcagtaaaca atttggctaa
      721 ataagattca gttgttatac gagaaggtta cggttcttct taactaagct tagtatgttt
      781 ttattcaagg catgattttc tctatgtgat cattcagttt ctgtaagagt tctaataatt
      841 cttttttgga gtacttgtaa ttgctttcta cccaccgatt gagaacggat agacaacctt
      901 ggacgtggta cgtagtaaag aatctcattt cttctgtgat aatcgttttc ttgtcttcat
      961 atagccatat gttcagatag ttttcttcaa taacttgttt aactttcttc tgaaacgaaa
     1021 tatcaccatg gttgaggata aataagcgat ataaaaaagt atttgcgtaa acatagtcaa
     1081 tcaagcgagt gtatagttcc tcatagctat gggctggatc ataagagaga atttcattta
     1141 gatcccgaaa aaagttgctt tctaattgat
//
LOCUS       DAZKOK010000253         1169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000253 DAZKOK010000000
VERSION     DAZKOK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.263"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..744
                     /gene="vanR-A"
                     /locus_tag="ACG90W_002933"
     CDS             49..744
                     /gene="vanR-A"
                     /locus_tag="ACG90W_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0479216.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            722..>1169
                     /locus_tag="ACG90W_002934"
     CDS             722..>1169
                     /locus_tag="ACG90W_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HFQ0479217.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAK"
BASE COUNT          402 a          206 c          245 g          316 t
ORIGIN      
        1 taatctgata aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa
       61 atacttattg tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac
      121 gagaattata cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag
      181 tctgagattg accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc
      241 tgtcaaaaaa taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca
      301 gaggtagata aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt
      361 cgcccactgg agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt
      421 ggagtaaagg agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac
      481 acccatgagt gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata
      541 ctgcgaatcc tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag
      601 atatggggcg acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat
      661 ttgcgcgaaa aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg
      721 gttggttata aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta
      781 catgtatatc gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat
      841 gatccgaggg aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa
      901 tcacctggac gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat
      961 ttatgtggcg attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc
     1021 aaaatacttt gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca
     1081 aattgagctt tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac
     1141 tctggaaaag cgagagcagg atgcaaagc
//
LOCUS       DAZKOK010000254         1149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000254 DAZKOK010000000
VERSION     DAZKOK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.264"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..970
                     /locus_tag="ACG90W_002935"
     CDS             278..970
                     /locus_tag="ACG90W_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0479218.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          494 a          160 c          184 g          311 t
ORIGIN      
        1 tttttgtaat aaatacagta atctatgaaa tgaaaagggt tacgtttaat aaaacttgct
       61 tttttgtttt tgttcaccat aaatatttat ttaagaaagt attaacctgc atttttctct
      121 ataaaaaaga ttttttaaat agataaaaaa ggaggattaa atgagtattt gatagtattt
      181 atattaaaat tacatatgta atttaaaaac gcttgacatt gtgtttgaga tcacttactt
      241 ttagcatgta aaagtattgg attaaggagc tatgcaaatg aaaaagaaat ttattactgg
      301 tgggattgtc gttgcatttc tatgtctaat tggctacaca ggcacgcggt acgcaaatga
      361 aattattttg tggggtggag agacagatat tgatacaatc aatgagaatc tgacacagct
      421 tgatcataca ttgacagata aagagcaagc gattacagat cttaccgctc gactagcaca
      481 aaaagaacag acattgcaag attcaaaggc agatgtccaa gaataccaaa caaaagtaaa
      541 tgaattagaa aaccaaaaaa atcaatcggc agcagaaaaa caaaacttag aaagtcaatt
      601 agtcagcaag gatgcacaac tacaaagcaa acaaaatgaa atcaatgtca aactagatga
      661 gatcaaccaa aaaaatgatg aaatcaatca tctgaatgca gattggacag caagactaca
      721 agaagcacaa gcaaaacttt cagaattaca aaatactgta aatggtttga atcaacaaat
      781 cgcaacactg acacaagaaa aaacaaatgt agaaactcag ttaacagata cacaaaagaa
      841 attagacgaa gcaactgctg aaaactcaag cttgaaacaa tatattcaaa agttagaaaa
      901 agcaaaagat gaagtggaag atacagctga taaatctcag caaattgttg aagaacattt
      961 gcgtaaataa tctttcttaa caagtaaacc taaattagta aagaagcaac agtaatctat
     1021 actaagtagt tcagttttgg agtacaaaat gacaaaatgt acttcaaaat ggactacttt
     1081 tttcatttta acttacatac caaattacat acaaaatgat ttataaaatg caattgaaaa
     1141 tgataattg
//
LOCUS       DAZKOK010000255         1095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000255 DAZKOK010000000
VERSION     DAZKOK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.266"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG90W_002936"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG90W_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          383 a          199 c          220 g          293 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga agttctgcac
     1081 cttataaaat ttgtc
//
LOCUS       DAZKOK010000256         1081 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000256 DAZKOK010000000
VERSION     DAZKOK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.267"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG90W_002937"
     CDS             283..522
                     /locus_tag="ACG90W_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479219.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..>1081)
                     /locus_tag="ACG90W_002938"
     CDS             complement(884..>1081)
                     /locus_tag="ACG90W_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479220.1"
                     /translation="KVVGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYTHVY
                     ELETTEYLYNYSNKYEHTLVS"
BASE COUNT          350 a          204 c          154 g          373 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 t
//
LOCUS       DAZKOK010000257         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000257 DAZKOK010000000
VERSION     DAZKOK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.268"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG90W_002939"
     rRNA            <1..>1073
                     /locus_tag="ACG90W_002939"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKOK010000258         1071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000258 DAZKOK010000000
VERSION     DAZKOK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.269"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..847)
                     /locus_tag="ACG90W_002940"
     CDS             complement(38..847)
                     /locus_tag="ACG90W_002940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0479221.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          323 a          202 c          189 g          357 t
ORIGIN      
        1 ttgtcacaac ctcttttttg tggaatctaa ttctctatta tagtttaagt agtttttcca
       61 gataaacagc taaaccgtct tcctgattgc tcaaaggagt gacatcattt gcaatgcctt
      121 tgagttggtc agtaccgtta gccatagcta ctccccaacc tgcataatcc aacaattcca
      181 aatcattatg ctcgtcacca aaggcaatga cgtctttttg atcgatagac aggtagttag
      241 caatctcttg cacgcctttt gctttttgga ttccttttga aacaatctct aagatggcag
      301 taggtccacc ccatgtccga acatcgacat aactgccaaa ttgacgtgtt aattcagcag
      361 atacagctcc tgcaaaacgt ttgtctgttc ggacaagcaa agatgtagga tttgtcgtca
      421 aatttttagg agaaagcaaa ttcttctctc ctggacgaga agaagcaaaa attttttcat
      481 caaagaaatc aaaactgtca ataaaaaaag tatcacgatt ttcagcagcg ataaaatcta
      541 aatttagctg ctttttttgc gataagattt caaaagcgat ttcacgattg atcaacgttt
      601 ctctttcacc atcccagtgc tgattaggga tgtgtactaa tgcgccgtta aaattaacca
      661 tcggtgttgt taattctaat tgttggtaaa actgatggct cattcggaat gggcgtcctg
      721 tagcaatact gacatgatga ccggcattga ttgctttttt tagagtctct tttgttttag
      781 atgtaatcat cgaatcttga ttgagggtag ttccgtccag atcgatggca attaattttt
      841 tattcaataa gggtccttcc ttcctcaact agcatttgtt ttagtctagc atattttagg
      901 taattaacaa gagcaatcag ctattcctaa caaaaactac agttatggct atataaatag
      961 ctaaacataa gctaaacacg aacgttaagt attattttcg ctttttttgt taaaaaaacg
     1021 ttgaaaagcg aatgaaacgt ctatataatt tgaaattgtt cgatggattt g
//
LOCUS       DAZKOK010000259         1068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000259 DAZKOK010000000
VERSION     DAZKOK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.270"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..>1068
                     /locus_tag="ACG90W_002941"
     CDS             5..>1068
                     /locus_tag="ACG90W_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0479222.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILT"
BASE COUNT          332 a          168 c          173 g          395 t
ORIGIN      
        1 aagaatgaaa ataaataaaa aagtagtaat attgagtgga ttaattatag ttagtagtat
       61 atattctttc atctttttca aaaatacaca gagtatttat accactggag atctttcttt
      121 tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta attatgagac
      181 attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt tttttccagc
      241 tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt acgtttggtt
      301 attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt taaaacaaaa
      361 aaaagcggca tttttattta gttgtctcta tattttctct gcttatcgga cagtagacat
      421 ctactatcgt tccgcaattg ccgaagctat cgcaataaca cttgttatcc ctgtattatt
      481 ttatgcttat caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa agctagcatt
      541 gagtatgagt ttgctagtgt acagccatgt actatcaaca ttaatgagta cagcattaat
      601 tatcattttt atttttattc gtttggtttc taaaggtttc aagaatgcag attttattgc
      661 tatatttaaa aaattattta gtgcggcagg aatgacattg gttctgacga gtgctttttg
      721 gtatccgatg tttgaacaaa tgctctacca aaaaatcaac aaacctagcg tgacaaattt
      781 atatgctcat gcatctaatg tgttcgatag tctgactgaa gctatgaaca acgacttaac
      841 tacttactca atgggactag ttggtctgtt atctttatgt attcccctga ttttatttaa
      901 aaaattaaca aacatagaaa aaaagattta ttatggtacg tgtttgactt ggctagctac
      961 aactagtttg gtgccatggt atcttctgca aaatacgcca gccaaattgt tacaatttcc
     1021 ttggcggatc ttaagcttgc aaataatatt ctcttctctg atattaac
//
LOCUS       DAZKOK010000260         1067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000260 DAZKOK010000000
VERSION     DAZKOK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.271"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG90W_002942"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG90W_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0479223.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DAZKOK010000261         1035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000261 DAZKOK010000000
VERSION     DAZKOK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.272"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..159)
                     /locus_tag="ACG90W_002943"
     tRNA            complement(86..159)
                     /locus_tag="ACG90W_002943"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Ile,seq:cat)
     gene            complement(714..1028)
                     /locus_tag="ACG90W_002944"
     CDS             complement(714..1028)
                     /locus_tag="ACG90W_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479224.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          383 a          160 c          163 g          329 t
ORIGIN      
        1 tttgagtggc aatgaagaaa agctttagct tgtataatac tcttcaaaaa attctaacac
       61 agaacgattc aattggctac attaatgtac cctgtaggac tcgaacctac gaccggacgg
      121 ttatgagccg tctgctctga ccaactgagc taagggtagt ggttgttgcc acataaagcc
      181 ataaacaatc aaccagtaga atgcgtggca acaaacctgt tatcgcatat cttggagtgt
      241 gactatttat gagtgatagt gaagatatgc gtctccatat atgtatggtt gagacagata
      301 aacatgtatg aaaaacgttg ttaaatcaac gtttttttat tttggtcaag gagagaaaag
      361 tagagaaata caaaattttt tgcattgatt ttgcattcaa aaaaacattc aaacatttag
      421 aaatccccaa tacgtattca ggaaaatcta ttttaaggtt tacaaaaaga acttttgttc
      481 gtacactctt tttgaggtga acttttatgc tgatggaagg aaaaacacaa ttatggttta
      541 agcatgttcc ttcaaataag ttttttaatt ataaactcta gagaaattaa ttcatcctaa
      601 cataaaataa ttgttcactc aaaagttttt ctagaacaat taaaacagca ggcttcacat
      661 tcgctataca tttgtaagaa gtaataattc ctgtactaaa aggcataatt gctttaatta
      721 ttttttgact tctcttcaaa cacaagaagt agttgtgtaa taattgcaga aataaggatt
      781 ccaataatag atataaaatt tgacatggaa acgtctataa cattaaaaga ataaagtgtt
      841 gaagaaagta aactgattga aataacccct ccgtaaaaaa tagtctgtga atatacccac
      901 tttttttcag aagacgtact tcgtttggtt ctaaaaccta tccctgcatt tctactctta
      961 tcaaaaaacc aaatatttag aagtaataat attgctatta ttaagataaa attactgtaa
     1021 aaatccaaaa gctat
//
LOCUS       DAZKOK010000262         1034 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000262 DAZKOK010000000
VERSION     DAZKOK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.273"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(373..771)
                     /locus_tag="ACG90W_002945"
     CDS             complement(373..771)
                     /locus_tag="ACG90W_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0479225.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(768..1004)
                     /locus_tag="ACG90W_002946"
     CDS             complement(768..1004)
                     /locus_tag="ACG90W_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0479226.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          343 a          173 c          141 g          377 t
ORIGIN      
        1 atcattcaat taaacgacaa aaaaagtaag aaaaactgca aaattttttt ataattttgc
       61 ataagcagat tttgatggaa aaatgacgat ttttgataaa aaaccgttcg agagaggttt
      121 atcatttttt aaatctactt tgtataatat atattatgta aactaaaata tataaaaaat
      181 cagcccacta gttctaaacc actatttcct actattcact cttttgtatt ccttttaaat
      241 caattatttc aaaattaatc catattttcc atatttttat gaaaaaacta ttttgtatga
      301 ttaaacataa aaaaatcagc atattcctta atgacatgct gatctaattg agaaataaag
      361 tttttaattc gttcatttta ttgcggtttg tcgtaaataa ctggttatct tggactttag
      421 tgaatcgaaa tccaatgaac tagtggtaat cattatgata aaatcaacag cttcttgacg
      481 agaaaaagca actggacaat tattcaattg aagaaacaac aacatggcaa caagagcagt
      541 tcgtttattg ccattatgaa aaggatgttt tttagctaaa ttaattgcta aaatcgccgc
      601 tttttcatag atttcaggat agagttcttg attgaatact gcttgtgaag gctgtttgac
      661 ggccatatct aatgcatttg catcttttac accgatcatt tcgcctggcg agtgacgtaa
      721 aataactttc gcgttgattt tgataatttc tttagtcgaa agatacctca taaatcaacc
      781 aaatctttta acgcgtcttc atattgattc atgatcgaat caactaatga atcaatatca
      841 ggttcttctt gtgctttgat cattcgtaca gaaccatcag attgaaaaac ataactgatt
      901 gcgtcacctg tctgcacacc catgaattct tttacttctg gtgggatagt tgttacatcg
      961 ctattgccag attttctaat tttatacttt ttcatttttt tcatatttcg ccaccctcta
     1021 taccattctc attc
//
LOCUS       DAZKOK010000263         1029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000263 DAZKOK010000000
VERSION     DAZKOK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.274"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1029
                     /locus_tag="ACG90W_002947"
     CDS             <1..>1029
                     /locus_tag="ACG90W_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479227.1"
                     /translation="WYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHI
                     NRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIV
                     TLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLIL
                     LGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLI
                     IKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSEL
                     LSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNII
                     QAPWRFMIF"
BASE COUNT          328 a          138 c          141 g          422 t
ORIGIN      
        1 tggtatatga atttgaaaaa aattttttct gtaaaaatat ttatagttta tgcatttgtt
       61 agtttattat atttatttcc agcatttaaa aacatggaaa tatttggctt ttatgatacg
      121 gcatttcaca ttaatcgtgc gttaagttta gaatctattt ttagctcccc tattaatttt
      181 gaaacgttta gatcatatgg catgcaagta aataattttt acccatggtt aactctatat
      241 cctttatttt tattgatcaa gtttactaat ttagcaatag gctataacct ttttctttat
      301 attgttacgc tcattactct atttatatgt cattatgtaa tgtatgaaat taccaaaaaa
      361 catgttacta gctccttttt cgctattata tatacaacat cttcttttag gagtgtagaa
      421 atatttcttc gaggggcaat gggggaatta ctcgcaatgt caatacttcc tcttatttta
      481 ctcgggttta taaagcttta cgactcaaaa aaagaatctt gggttatgtt ggcgattagc
      541 atgactttat taatttacac tcatgttctt tctgtagcta tgactatggt aatgattata
      601 attgctttaa ttattcaatt ttatcggaaa aagaaaatag aaatcttctt aattattaaa
      661 ttaataaaag ctgctatagt tactttactt ctttcactag cgtttatagg tcctatgatt
      721 gagcagacat tgtatcaaga tctgaataga ccttatgtgg atatattaca aaaaagagca
      781 atttatctgg gtaaagagtt tttgattgga agtatagatt cagaattact atcatttggt
      841 attggcttaa ctttgcttat ttgtttaatt atactaacat ttaactttaa aaaattagga
      901 atcttatcta aaatcactta tataatgggt ataatatcta ttctactttg tacaaaaata
      961 tttccatggt ttgccttaca aaatactcct ttaaatatta ttcaagctcc ttggcgtttt
     1021 atgatattt
//
LOCUS       DAZKOK010000264         1027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000264 DAZKOK010000000
VERSION     DAZKOK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.275"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..858
                     /locus_tag="ACG90W_002948"
     CDS             <1..858
                     /locus_tag="ACG90W_002948"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0479228.1"
                     /translation="NIIHMHMSYRGSFSRKYVLNKWFRLLNIPVIIHLHGSEFKKWYD
                     SVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPEYTIKWNS
                     KMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLKELIEEYN
                     LQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIATDVGDIS
                     SAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDGNFFDRIL
                     NLYQKGSKE"
BASE COUNT          381 a          110 c          220 g          316 t
ORIGIN      
        1 aatattattc atatgcacat gtcgtacaga gggagtttta gtaggaaata tgtgctgaat
       61 aagtggttta gattattaaa tattcctgta atcatccatc ttcatggatc ggaatttaaa
      121 aaatggtatg atagtgtttc tgtaaaaaaa caaaatcaga ttcgcttgct gttaagagag
      181 tctgattcca cgattgtact tggagaaaaa tggaagaaga taattaaaga aattgagcca
      241 aattgtaaaa ctgttgttgt agaaaattct attgtagttc cagaatatac cataaagtgg
      301 aattcaaaaa tgtgccgatt tctgtttatg ggagttttga taaagcgaaa aggtgtacac
      361 gatttggtag aagcagttaa aaacattgca gaacaatatc cagaaaaaaa gtttattttt
      421 gagattgctg gagcaggtga tgaagaaata tcgctcaaag aattgataga agaatataat
      481 ttgcaatcgt attttgaatt tacaggttgg gttgtgggag aaaaaaagga ggagtgctat
      541 agaagatcaa atgtgatgat tttaccgtcc tataacgagg gattgcctat atctattcta
      601 gaggcgattt cttatggaat gcctgtaatt gctacagatg taggagatat ttcttcagca
      661 gtacgtgatg gagaaaatgg atatttgttt caacctggtg acgtatcagc gttgacatct
      721 gcaatactaa aaatcaatga taaagataaa tacgtaaaat tttcacgttg ttcgaaggat
      781 ttggcaataa cgaaatttaa cgatggaaat ttctttgata gaattttaaa tctgtatcaa
      841 aaagggagca aggagtgatg acaaggaaaa taaaaaatgt ttcgattatg taatgaactt
      901 attaagtgaa taaaatagag agaaaaaata ttgaaataga cgatgtaaat ttttatccgt
      961 gtaagaattc aaatgaatag tagtgtttta gtgcttgtaa tagagcaaga gtacaagcac
     1021 ggtacat
//
LOCUS       DAZKOK010000265         1026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000265 DAZKOK010000000
VERSION     DAZKOK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.276"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG90W_002949"
     CDS             complement(<1..434)
                     /locus_tag="ACG90W_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479229.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNN"
     gene            complement(441..989)
                     /locus_tag="ACG90W_002950"
     CDS             complement(441..989)
                     /locus_tag="ACG90W_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0479230.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          358 a          202 c          102 g          364 t
ORIGIN      
        1 aaattatttt ttaatagaaa ctgaaaatta aaactagaaa aagtagtttt accgaatgta
       61 agagcatcat caccatcaat atagaaatac attcttttcc agttacattt aatttttaaa
      121 caaaattctc cccaaaaagg aactttttct agtgaaaact tggtaatctc tttgtaatta
      181 attcttgtta aagaataatt aaacacttca tttttacttg ttattttttc attgactata
      241 actagttcat ctggtaaaaa aattaaccaa caaacatcga caaacagtaa atgatataat
      301 ccatttccac ctccaagcat attcaatact ggtgaacatg ccttagaaac acgaatatag
      361 ttatggttta aatagatatt ttctttaaac attgcttgtt taaaagccat atgctcttgt
      421 tctaaacttt tcataaactt ttactctaat gcacagctag cagcagctgc tgcaattcct
      481 gctacaatat ttaccttagc taactcttta gctgcagctt ttcctaattg tttttctgca
      541 atttttaata ataaatccgc agctttttca aaattaaaag cttttaatgc ttcaataaaa
      601 tctccctgcg tacctatagc ccaagccact cctcctccag gaagagaatt aatggctaca
      661 catccagcat atcgtttcca atcaactttc tccattctag gaacaataat atgttcataa
      721 aagactcttg ctgctcttcc ttgttcgtta tcttgactga ttcgttcacg aatagcacca
      781 aaatccttta tatggtatac accatgttcg tctaaaaaac caactgtatc aaaataaaaa
      841 gttaattcat ctactaattt attctttatt tctaaatcaa ctgtattcac tttttctgtg
      901 gcatacacac ttgttccagt actaacaaaa gtacttaaac ctaataagaa aacacctaaa
      961 acaccaaata ttttaattat tcttttcata atttctcctc cataaaaaat aagttacttt
     1021 ttaaat
//
LOCUS       DAZKOK010000266         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000266 DAZKOK010000000
VERSION     DAZKOK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.277"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG90W_002951"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG90W_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKOK010000267          980 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000267 DAZKOK010000000
VERSION     DAZKOK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.278"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..803
                     /locus_tag="ACG90W_002952"
     CDS             <1..803
                     /locus_tag="ACG90W_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0479231.1"
                     /translation="SPKEQAIADYILENPSKVSHSPISNLASELGIADSTFFQFTKKL
                     GYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFESSISTLNDTKKLLQQ
                     KDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHSTDYHIQLMEASLL
                     TEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAKLGDVVFISISEET
                     EFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISEIKE"
BASE COUNT          328 a          176 c          173 g          303 t
ORIGIN      
        1 tcagcccaaa agaacaggct atcgccgatt atattttaga aaatccaagc aaagtaagtc
       61 atagtcctat tagcaattta gcaagtgaac tgggcattgc tgactctact ttttttcaat
      121 ttacaaaaaa acttggttat aatggattta aagattttaa aatggccatg ttgatccaag
      181 aaaatgatct atctgctatt tctatccacg aaaatattca gaaaacagat actgagctca
      241 ctatggcgca aaaggtgttc gagtctagta tttcaacttt aaatgacaca aaaaagttgt
      301 tacaacaaaa ggatctaaaa aaagcggcta ctattatcaa tgaatctaaa cgtgtctatt
      361 ttttcggtgt tggcggttca gagattgtag caactgacgc ttatcataaa tttttgcgtt
      421 cccctatcgc aacttctcac agcacggatt accatattca attgatggaa gcttctttat
      481 tgacggaaaa agactgtgct gttttgattt ctcacacagg gcaatcaaaa gaaacaatcc
      541 atattgctga aaccgtaaga aaaactggag caaaaacaat cgttattact agccaagcta
      601 attcttccct tgctaaactt ggcgatgtcg tttttatttc tatttctgaa gaaacagaat
      661 tccgatctga agctttagct tcccgtattt ctcaactaag cattttggac tctctatatg
      721 tgattttgat gttttacaac gaaaaagaat cacgtcgttc tatcgctaaa gttcgacata
      781 ctatttctga aatcaaagaa tagcaccata acagaaaaag cgaaaaatcc ataaaacaaa
      841 aaaagccact accaaatatt tggtagtggc ttttttggtt ctgcaagggt ggtacagaac
      901 atcaatagtc ggtgggttag aacgtatgtc atcgcatctt tggagaaggt tgtattggta
      961 ttattcgagt tacttgttag
//
LOCUS       DAZKOK010000268          972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000268 DAZKOK010000000
VERSION     DAZKOK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.279"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..635)
                     /locus_tag="ACG90W_002953"
     CDS             complement(<1..635)
                     /locus_tag="ACG90W_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0479232.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSK"
BASE COUNT          321 a          186 c          104 g          361 t
ORIGIN      
        1 atttttgaat gttgcaaata tgaaatatgg aatatgatat cttcagatat ataatccctt
       61 tctgacggaa acttgatatt gtgctgttga attatctcag atgaatacag cgcatgccaa
      121 actgacattg gcaatataaa atcgacttta taactcggct cagtgccaat cattccaagc
      181 aacacttttt caattatagc gttatttgta aatatcgtac tatcatatct tgctggcttt
      241 gaaataatat tattatcatc ataatattca taatatccag cataacatgt gtctgcatta
      301 ctccttttta taccatttaa cattgcttca atataattag ctgcaacaaa atcatcagaa
      361 tcaataaaag caacatattt tccttttaca tgttcaagtc ctgtatttct tgccaatccc
      421 aagccagcat tatttttatg aataatacga atccttgaat cctttaatgc atagtcttca
      481 catattttat tagatgaatc cttagatcca tcatccacaa ttataatatc taggttctta
      541 tatgtttgat tacacactga ttccaaacaa cttcctacat acttttcaac attatataca
      601 ggtatgatta cactaattag atcactcgaa ttcatccttc tatcctttca ctcacgtttt
      661 ctcaattagt caatgacggc atcaacgatt ttcttaaatt ctctgaaaaa aatagactct
      721 gatcttgatg atgtgaatga catactatct tcttctagaa aaattatttc ctttaaatta
      781 tttattaaat caataatatg atcctcgtaa gtatgctttc ccttggaata atgaaaatat
      841 acttttgtat tacacaatat tgcaatttta tttcttaata tgattatttc actcattcat
      901 tactgccaca tatacacaac ttataaccac cccctgcaaa gtttagggta agttatcaga
      961 taatatcttc ct
//
LOCUS       DAZKOK010000269          928 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000269 DAZKOK010000000
VERSION     DAZKOK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.280"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..640)
                     /locus_tag="ACG90W_002954"
     CDS             complement(<1..640)
                     /locus_tag="ACG90W_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0479233.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLF"
     gene            complement(651..>928)
                     /locus_tag="ACG90W_002955"
     CDS             complement(651..>928)
                     /locus_tag="ACG90W_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFQ0479234.1"
                     /translation="PTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLIRATMI
                     AEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
BASE COUNT          324 a          155 c          127 g          322 t
ORIGIN      
        1 caaatagaac ttgatcaatt aattctttca aatagtaaaa acgatgttca gctataacaa
       61 tagtaaatcc tttatttttt aaatcacaaa gtaagtcagc aagtttctta gtcgctttga
      121 agtccaaatt tgaagacggt tcatccaata taattaattt agagtttagc gataaactac
      181 atccaataga aattttctgc cgttcaccac ttgatagcga aaaaatgttt ttatctaata
      241 tatttgaaag atctaataat tcagctaatt cattgattct tttatccata tgctcttttg
      301 taaatccaaa gttttccatc gaaaatacta actcactcgt tgtatttgtt gtaaagaatt
      361 gtcctctagg atcttgactt acgtagccaa tattttttga tatactttct tgtgttaagg
      421 attgtagcga gttattttca aataaaattt ccccaaccaa ttttccgtca taaagtcttg
      481 gaataagtcc attcattaac tttaatagtg tggatttccc actcccagaa gaaccagtaa
      541 tcaatgtaag actgcctttt tttattgaaa aattaaggtc aacaagcgct tgttccttaa
      601 catccttata tgaaaaagaa acattgttaa attcaaacaa ttctttcacc tcaaatcaaa
      661 atacttaaga aaaaaatagt aacggaaaac aggatgatat agtctaataa gctcatgttt
      721 gttttatagt aagaacttct tttcacagga gattctatcc cctttgttat tgcattagcg
      781 gtcatttctt cagcaatcat agttgcacgt ataagaacgg gaactaacag aaattccaaa
      841 tattgcagag gctgaaaaaa agacaagcga atgtttctca tttttaaaga ttctttaatc
      901 attcttattt cagaacgata agtcggaa
//
LOCUS       DAZKOK010000270          910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000270 DAZKOK010000000
VERSION     DAZKOK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.282"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG90W_002956"
     CDS             <1..355
                     /locus_tag="ACG90W_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479235.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG90W_002957"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG90W_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAZKOK010000271          902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000271 DAZKOK010000000
VERSION     DAZKOK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.283"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..282)
                     /locus_tag="ACG90W_002958"
     CDS             complement(<1..282)
                     /locus_tag="ACG90W_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479236.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
     gene            complement(384..737)
                     /gene="tnpB"
                     /locus_tag="ACG90W_002959"
     CDS             complement(384..737)
                     /gene="tnpB"
                     /locus_tag="ACG90W_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0479237.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          258 a          185 c          158 g          301 t
ORIGIN      
        1 tttcgttcct acaactcgtt gatgtccttt tactggaata atggtggcag ctgcttctgg
       61 aacacttaca gaaatttctt catcactaaa aagattcaat tgcccattcg tttcctcaaa
      121 gagagtctct ttagagcgtc caaataattt gttggttaaa taattgacct tttcattcaa
      181 tagttggatt tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat tggttttatg
      241 ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc accaccttga
      301 atcgaactga ttttgttaca gaataccaca agaaacgcac aagaaatagg ggttcaacac
      361 attttttgtg ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga
      421 ggttgatcga tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga
      481 acttcgtctt tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga
      541 agaaaaccat caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat
      601 aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga
      661 ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga
      721 agtaaatcat aaagcattgg acaacagttc tttcaaaata gcttgaagaa tataattgtt
      781 aataccgtta tagatcaaaa cttcagaacc attctctctt ttgatgcgca tcgctaactt
      841 ggcagccgcg ggtttacctt tttctggtgc actcttttcg tttcctaatt ggacaggaac
      901 aa
//
LOCUS       DAZKOK010000272          896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000272 DAZKOK010000000
VERSION     DAZKOK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.284"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..528
                     /locus_tag="ACG90W_002960"
     CDS             <1..528
                     /locus_tag="ACG90W_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479238.1"
                     /translation="ENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIF
                     AKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPIST
                     ISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLP
                     ENWAELRKLVEYGII"
     gene            515..>896
                     /locus_tag="ACG90W_002961"
     CDS             515..>896
                     /locus_tag="ACG90W_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0479239.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLL"
BASE COUNT          306 a          120 c          133 g          337 t
ORIGIN      
        1 gaaaacagta aattgatgaa aaacatacaa tcaatctttg ggatagaaga agtttttgtc
       61 ggagataaat gtatcttttt tgatcaacct ttattatctg atggttatgg catagatgaa
      121 acagatattt tcgctaaagt aaatagtata ttttctggaa aaaaagaaac tttaattaag
      181 cttcatccta gagcaaggac aaatcgttat ggtgaaaaaa acatattgaa ttccagttta
      241 ccgtttgaag tcttgctact tgaaaatgac ttctcttcaa cacttttgat tagtcctata
      301 tctacaattt ctttttcacc atctatgatg tttgaaaaga cgatcaactc tattatgttg
      361 gctaaattaa ttttaaatga atatacagca ttatctgaag ataaaaaaga aacattgaca
      421 aatattgttc ttttctgtga acattataac cgtctatcag aagaaaaaat tttactccca
      481 gaaaactggg cagaattaag aaagttggta gagtatggga ttatttaaca cactttccaa
      541 gtcaaaatta gttaagtcca gtttctggta tactataggg aattttttta ttaagggaat
      601 ttcattcata actattccta tttttgtaaa tattactact caagcagaat atggtttgat
      661 taataatttc acttcatttg tatcaatttt ttcgattctt gttgggctaa gcctaaattc
      721 ggcaattaat aatgcaagtt ttgactataa agatgacata aatgaattta tgtcatcggt
      781 actttctcta gctacacttt cgttaattgt gttcttatta atagggaaca tatatttttt
      841 gttttcaaat gctttttttg atatgaacca acttgtattt aatttgatgt tattac
//
LOCUS       DAZKOK010000273          894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000273 DAZKOK010000000
VERSION     DAZKOK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.285"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..875
                     /locus_tag="ACG90W_002962"
     CDS             252..875
                     /locus_tag="ACG90W_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0479240.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          329 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aaga
//
LOCUS       DAZKOK010000274          889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000274 DAZKOK010000000
VERSION     DAZKOK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.286"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..391
                     /locus_tag="ACG90W_002963"
     CDS             62..391
                     /locus_tag="ACG90W_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0479241.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            381..668
                     /locus_tag="ACG90W_002964"
     CDS             381..668
                     /locus_tag="ACG90W_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0479242.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
BASE COUNT          354 a          110 c          155 g          270 t
ORIGIN      
        1 aaaatagcgt acaatgtaat tactaagtaa ttacgaaatg taaaataatg gaggaaataa
       61 tatgattaca aaaacaagaa aacaaggaaa ttctattatg ttaacggtcc ctaaagaatt
      121 cgatgtgccc aatggtgttg aagtagaagc taagcttgta gagaatggta tcctttatga
      181 gtttgtagag cctaaaaaag aattttttga ttttagtgaa gatattttgg ctgatattct
      241 ttcagaaggt tatagcaaac aagagatctt aaaagaattt agaaatagaa aaaatgcgtt
      301 aacttctgca tttaaatcta ttgctgaaga tactgttgca aattctaaac cgatgacaaa
      361 agaggagttg gcagcagaaa ttggcttatg agattctacc ttctaaacac gtaataaagt
      421 atctaaaaaa attaaaagag aaaacattaa aagaacaatt tttaacgata atttatgatg
      481 agatagcagt tcgtcctcat tcgggagaac aaaaaacggg tgatttatca ggaatttggg
      541 ctattggatt caagtatgca ggtacgacat atcgcgttgc atatgaaatt aaagataatg
      601 cagtgatacc taacttacta tgtggaacgc atgaaaattt ttatgaacaa ttaaaaaaga
      661 ttaggtaaga acccaatgtt tcaacttgag aggatagacg acactttaaa agataatttt
      721 taacagctga gagttgtaat accctataat tttaaaccac taacctatat atttatgaaa
      781 aatagaaaaa attaaaacgc attttaagcc tctttcaaat taaaagcttc atccttatgc
      841 acaaagaggg cttaaaatgc gttattttat attgtttttt agtaataat
//
LOCUS       DAZKOK010000275          862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000275 DAZKOK010000000
VERSION     DAZKOK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.287"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            437..835
                     /gene="spxA"
                     /locus_tag="ACG90W_002965"
     CDS             437..835
                     /gene="spxA"
                     /locus_tag="ACG90W_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0479243.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          328 a          116 c          147 g          271 t
ORIGIN      
        1 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
       61 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
      121 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
      181 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
      241 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
      301 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
      361 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
      421 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
      481 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
      541 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
      601 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
      661 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
      721 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
      781 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
      841 taatacgaaa cttggaaaag ac
//
LOCUS       DAZKOK010000276          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000276 DAZKOK010000000
VERSION     DAZKOK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.288"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG90W_002966"
     CDS             <1..672
                     /locus_tag="ACG90W_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0479244.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKOK010000277          806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000277 DAZKOK010000000
VERSION     DAZKOK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.289"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..794
                     /locus_tag="ACG90W_002967"
     CDS             162..794
                     /locus_tag="ACG90W_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0479245.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          284 a          116 c          131 g          275 t
ORIGIN      
        1 aattacgcct aatttgtgtc tctcagggca attctaagaa cgtttcaaga gatagcgaca
       61 ctatcgttat cgaaaaaggc gaaaacctat ctcattttct gctaatgtaa gttggtttag
      121 cttttgttaa tatgacttta tgaaaatgga ggtaattaaa tatgattttt cctaaagtta
      181 atgaagcaag cttgaataaa ggatcctttt ttcatatcta tgaaaacgaa gattttgaac
      241 tgtataatat cctttcacaa aaagaggtca tcgaaaacca aataatctgc catccaatta
      301 tttacaaatc tattttcaag tatgcatttt ttgcttccaa tgattttcaa agaatggaga
      361 ttaccgactg tatattcgaa aactgtgatt tttcaggctg tatattgaca aaaagcatgt
      421 tgcatcgcgt gaaatttgtc aattgtaaat taacaggtac taaattgatg gaaagttata
      481 ttgggaacac gttattcgag aattgtaaaa tggactatgt aaatttatct ggttcaaaca
      541 taaaagaaag tacctttgag cactctgtct tatcaagcgc agattttgta gactgttccc
      601 tcactaaaat aatgttttct actaatgatt tagaaaatgc tagctttttt gatactgatt
      661 taaaaaatat cgatttaagt agcaactcct ttgaaatgat tgaagtacgc tcggaacatc
      721 taaaaaattg tacgcttaat cagttgcaag cacttggttt tgctagaaaa tttttacaaa
      781 taaaagtagt ataatcaaaa tgaata
//
LOCUS       DAZKOK010000278          792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000278 DAZKOK010000000
VERSION     DAZKOK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.290"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>792
                     /locus_tag="ACG90W_002968"
     CDS             <1..>792
                     /locus_tag="ACG90W_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0479246.1"
                     /translation="AHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLV
                     LPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRK
                     PNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKV
                     IITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFA
                     DPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT          269 a          137 c          153 g          233 t
ORIGIN      
        1 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
       61 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      121 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      181 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      241 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      301 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      361 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      421 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      481 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      541 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      601 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      661 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      721 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      781 aaacgaaata at
//
LOCUS       DAZKOK010000279          757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000279 DAZKOK010000000
VERSION     DAZKOK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.291"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..180
                     /locus_tag="ACG90W_002969"
     CDS             <1..180
                     /locus_tag="ACG90W_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479247.1"
                     /translation="SQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAII
                     TQLFLVFGEKKRSKK"
     gene            complement(384..749)
                     /locus_tag="ACG90W_002970"
     CDS             complement(384..749)
                     /locus_tag="ACG90W_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479248.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          303 a           93 c          136 g          225 t
ORIGIN      
        1 tcacaaacta ttttttatgg aggaattgtt ttaattagtt tactttcttc aacactttat
       61 tctttgaata ttatagatgt ttccacctca aattcaatat ctattattgg aatcattatt
      121 gctgcaatta ttacacaact gtttttagtg tttggagaga agaaaagaag taaaaaatag
      181 aaaaactagt gatagttttg gtgatagtga tattaaaaat cagaaaagat agaaaaagca
      241 ggagatagat aaaatgctga tatttcagca ttttttaaga gtataaaaga gaaaaaaagt
      301 aataaatgta tacatgatat aagaaaaaca tatatgtata aaaagaagaa agtagcttct
      361 ctttgtacat atatggtttg tttttatgga tgccgagctt ttttataaga taaggctacc
      421 gcccataacg acccaaggat ttgtggaact aagtaaatac ctgcaagaag caagatcagc
      481 tgggtcggtt ttaaaggatg atagaagtaa cgccattcag taaaattggc gcaaaataaa
      541 taggaagtga cagtagatat aatattggca atgaataaag atataatacg cctgttcctt
      601 tgaaaataat atccagcaaa aagagataaa aatatcaatg tgatgaaccc gcttacggaa
      661 tgataacgaa aatcaagcag taaggcgaaa tgcacataag gaacaaaaga taaaagcaac
      721 ggaaggtttt tccagcaagt ttttatcatc tttcatc
//
LOCUS       DAZKOK010000280          757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000280 DAZKOK010000000
VERSION     DAZKOK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.292"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..>757
                     /locus_tag="ACG90W_002971"
     CDS             50..>757
                     /locus_tag="ACG90W_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFQ0479249.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNS"
BASE COUNT          299 a           96 c          128 g          234 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattct
//
LOCUS       DAZKOK010000281          755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000281 DAZKOK010000000
VERSION     DAZKOK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.293"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..666
                     /locus_tag="ACG90W_002972"
     CDS             28..666
                     /locus_tag="ACG90W_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0479250.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          238 a          120 c          124 g          273 t
ORIGIN      
        1 aaaaactgaa tagtaggagt tattatcatg aataacattt taggaaaaaa aatagaactt
       61 aaaaaaggga tattgggcac tattgtcgtt gtactcggtt tgttagggtt gctatttgcg
      121 ccatccatta ttagcatttt ttctttaact atcactggat ttatagcatt tacacttgtt
      181 tttggtaaaa aagatacgaa aaaaatgttc tcgaaacctg tcgctcctgt caaaaatata
      241 gccaaatatt tttttatcaa tgtagtaatc agtgccgcag tctcggtatt tttgcaacaa
      301 attttgaaat ggggtttaac aggcaatcca ataaatgagg ctttcagccc gttattattt
      361 attattttgc ctatcatgat acttggagaa gaattgttct cagtgtattt tctcgctatt
      421 ttttctagta agttcagcat tcctgtcgct agtttcttgt cagcaatcat ctttggattt
      481 atccattaca gtacttatga taatggaaat attttacaca ctgtagcaca catccttttg
      541 atacaagggg tggcaagatt gttattcaac caagctgcca ttaaaagcaa ctcgatcatt
      601 acaagttggg cagttcatgt gatatttgat ttcacagcta tattgctagt cgttttattc
      661 tcctaaattt ttatagaaat aatacatgct ctattgctaa gaaagcatac tttatttcct
      721 ttaaacaaaa agacagatgt atcaatggat taaaa
//
LOCUS       DAZKOK010000282          731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000282 DAZKOK010000000
VERSION     DAZKOK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.294"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACG90W_002973"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACG90W_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          268 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa a
//
LOCUS       DAZKOK010000283          726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000283 DAZKOK010000000
VERSION     DAZKOK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.295"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>726
                     /locus_tag="ACG90W_002974"
     CDS             <1..>726
                     /locus_tag="ACG90W_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0479251.1"
                     /translation="TENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDI
                     NFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQ
                     FFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLS
                     KTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNG
                     GIKKDIKLRVEDSTLIEQIFNDIT"
BASE COUNT          293 a           80 c          135 g          218 t
ORIGIN      
        1 actgaaaaca ataacgcatt aaggaaattt gacattttta atagttcgat tgaagttccc
       61 tatatcgaaa aggtaagtgc agatgtatta aaagttgaag aaattactta tgaaaatatt
      121 ctaaaagaca taaattttga tgtcaaagtt ggagatgtta ttggtttggt agggaaaaac
      181 ggtgtgggga aaacaacctt actaagaata ctcatgaaga ttattcgccc taccaaagga
      241 aagataatcg aaaataaaaa aaatttgtca tcgccgtttt tagtaatgca agaaatggat
      301 taccaatttt tcacagaatc agtgaggagc gagttagccc tggggaatga aatagttagt
      361 aaagatcagc aagaaaaaat tttaaaaaaa atggaacttt ataaaatgaa agatcaatta
      421 ccgtttgatt tatccggtgg agaaaaacaa agattattgg tttctatagc ttcattgtca
      481 aaaactaatt tatttctatt tgatgaacct acaagtgggt tggattatat taatatggaa
      541 aggattgctg accttattaa agacttaaaa gaaaagggtg cagtagttat agctactcat
      601 gatccggaat ttctttataa aacatgcaat agaattattt atttaaaaaa tggtggaata
      661 aaaaaagata taaaacttag agtagaagat tcaactttga tagaacagat ttttaatgac
      721 ataacg
//
LOCUS       DAZKOK010000284          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000284 DAZKOK010000000
VERSION     DAZKOK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.296"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG90W_002975"
     CDS             <1..>725
                     /locus_tag="ACG90W_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0479252.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKOK010000285          717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000285 DAZKOK010000000
VERSION     DAZKOK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.297"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG90W_002976"
     CDS             <1..608
                     /locus_tag="ACG90W_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0479253.1"
                     /translation="IGTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGT
                     GVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRL
                     GKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFA
                     KLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          201 a          142 c          182 g          192 t
ORIGIN      
        1 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
       61 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      121 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      181 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      241 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      301 aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      361 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      421 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      481 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      541 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      601 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      661 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DAZKOK010000286          701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000286 DAZKOK010000000
VERSION     DAZKOK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.298"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..>701)
                     /locus_tag="ACG90W_002977"
     CDS             complement(17..>701)
                     /locus_tag="ACG90W_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0479254.1"
                     /translation="VVMYLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDK
                     AYRLEQLIDEFFEITRYNLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAP
                     EDLTVSGDPDKLARVFNNILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDK
                     LAAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAM
                     PDLVDKRRS"
BASE COUNT          174 a          167 c          152 g          208 t
ORIGIN      
        1 aaattatata catctcttag gacctccttt tatcaaccaa gtctggcatc gctggaagct
       61 ctaccctaaa cgtcgtatag ttatcattgc tttccgcgta aatctgccct ccatgctgaa
      121 caataatttc ttttgcaatc gccaatccaa gtcccgcgcc acccgtatcg gaagaacgag
      181 cattgtccag cctatagaac ttttcaaata tggcagctag cttatctttt gggatgcttc
      241 cagtgttctt gaattcgatt gacaccacat ccccggagag gcccgcggta atgtcaatga
      301 tgctgttatc ctcactgtat gcagcggcgt ttttcaaaat gttgttaaag actctcgcga
      361 gtttatcagg gtcgccggac acggtcagat cctcgggggc gtgaataacc gcctgttttc
      421 catgtgcgga aagctgagga taaaattcat cggtcatctg caccagcata tagtataggt
      481 ctatgtgcgt ttttgttagc gttatcgttt gtaggttata ccgtgtaatc tcaaaaaact
      541 cgtcgattag ctgttcgagt cgatacgctt tgtccaacgt gatatgcaca tactttgcct
      601 tttgatctac cggcatgtct ggagcctcgt caagcaggct caaataaccg ataatggatg
      661 taaggggcgt tttaatatcg tgcgccaagt acataacaac g
//
LOCUS       DAZKOK010000287          700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000287 DAZKOK010000000
VERSION     DAZKOK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.299"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG90W_002978"
     CDS             complement(<1..563)
                     /locus_tag="ACG90W_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0479255.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT          188 a          146 c          116 g          250 t
ORIGIN      
        1 tattccctat tgtcttttaa gtagcggtag agtgttgcct tgctaatacc agtcatttcc
       61 gtaatttctt ttacgctata aacctgactt ttatacaatt ttactgctcg atcaacatct
      121 ttctggttca ctcgtggacg gccaccttta cgacctctcg cacgtgcact tttcaaacct
      181 tctttggtac gttcaacaat taaatcacgt tcgaactgac taaatgcttg gaagacagtc
      241 atcattaagc gtccgtgagg cgtgctggta tcgaagtttt ctttcaagct caccagctta
      301 acatggtgtt cttcaaaata ggtaacaagg tcaatcaaat cctttgtact acgacccaat
      361 cgagaaaaac tttctacaat taccgtatct cccggacgaa ttttatcttt taaacgattt
      421 agttggggac gaaccgcttt ggttcccgtc attttctcag ttaggatttc attacaattt
      481 tgttcattga gtaagtctaa ttgacgattt agttcttgtg aacgtgtact cactcgggca
      541 taaccataaa tgtatccact catgcttttc ttcctttcgt actttaattc tggcttgatt
      601 gtatcataaa agggagttat agatacatag ttaatgcaac aagttatgat acaaaatatg
      661 acctctttta tttaataaaa gaggtcattt cattttgtct
//
LOCUS       DAZKOK010000288          690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000288 DAZKOK010000000
VERSION     DAZKOK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.300"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG90W_002979"
     CDS             complement(<1..461)
                     /locus_tag="ACG90W_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0479256.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          219 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac
//
LOCUS       DAZKOK010000289          685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000289 DAZKOK010000000
VERSION     DAZKOK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.301"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG90W_002980"
     CDS             132..344
                     /locus_tag="ACG90W_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479257.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>685
                     /locus_tag="ACG90W_002981"
     CDS             506..>685
                     /locus_tag="ACG90W_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0479258.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRF"
BASE COUNT          245 a          102 c          120 g          218 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggttt
//
LOCUS       DAZKOK010000290          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000290 DAZKOK010000000
VERSION     DAZKOK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.303"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG90W_002982"
     CDS             154..417
                     /locus_tag="ACG90W_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0479259.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="ACG90W_002983"
     CDS             417..>670
                     /locus_tag="ACG90W_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0479260.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAZKOK010000291          626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000291 DAZKOK010000000
VERSION     DAZKOK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.304"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>626
                     /locus_tag="ACG90W_002984"
     CDS             <1..>626
                     /locus_tag="ACG90W_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HFQ0479261.1"
                     /translation="DKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADK
                     HPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLL
                     NFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDP
                     VSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIYITEFDDFNDYP"
BASE COUNT          240 a           77 c          111 g          198 t
ORIGIN      
        1 gataagctct attgggaaga aattaaggca agtataagaa tgaatgccac aaacttagat
       61 ggtgatagag aaattcgtta tagtataaat ttacaaaaac atttattgga aataaaaaag
      121 ttagcagata agcatcctaa ttcaggccaa ttgaaaaaag cactttctga gtttaataaa
      181 gaacgcatta ttaaaatcac ttcaaagccg aacgatcttt taccacttat tggtatcctg
      241 gtagatatca tgataaataa cccgggttct ttgccgcagt gtgtcttaat tatagggcag
      301 ttattaaatt tcgaaaatga gagtaaggct agagaaatag ttgaaatgat tataaacaaa
      361 tatgctttaa agccaaatac tgaatttatg atgatatggt ttcaaagatt gaccaccatt
      421 tttggagaaa tgtttgatag tgttttatca tccgaatttg tatctaaaat caaagatcct
      481 gtctctaatc agatgttttc tgtagactgg ctaaatgaag atgctaagaa atctttttat
      541 gaagggacca ttaaagacga agagaaatat attaaaatga gtaagaaaat atatattact
      601 gaatttgatg attttaatga ttatcc
//
LOCUS       DAZKOK010000292          588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000292 DAZKOK010000000
VERSION     DAZKOK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.306"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG90W_002985"
     tRNA            complement(65..150)
                     /locus_tag="ACG90W_002985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG90W_002986"
     tRNA            complement(167..238)
                     /locus_tag="ACG90W_002986"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG90W_002987"
     tRNA            complement(250..322)
                     /locus_tag="ACG90W_002987"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG90W_002988"
     tRNA            complement(340..421)
                     /locus_tag="ACG90W_002988"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG90W_002989"
     tRNA            complement(429..501)
                     /locus_tag="ACG90W_002989"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG90W_002990"
     tRNA            complement(505..577)
                     /locus_tag="ACG90W_002990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZKOK010000293          581 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000293 DAZKOK010000000
VERSION     DAZKOK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.307"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..485)
                     /locus_tag="ACG90W_002991"
     CDS             complement(<1..485)
                     /locus_tag="ACG90W_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0479262.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     S"
BASE COUNT          177 a          110 c           70 g          224 t
ORIGIN      
        1 caagaaaata ttgtttctgt ttttccttta tactgattgg cagctattat taatttttca
       61 ctcaataact ttgtcgcacc catggtgtta gtaggactca ctactttatc tgtactgata
      121 cctacaaaat gttttacatt atttttgatc gctgcatcta ttacattttg agttcccatc
      181 acatttgttt ttacagcttc cataggatta tattcacttg ctggaacgtg tttcattgcg
      241 gcagcatgaa aaataatatc cacatcacga catgcataat caatcctttg agagtctctg
      301 atatcaccaa ttaaaaatct caaattagtg tgatttgaga gctcttgttg catataaaat
      361 tgtttttctt cacctctact ataaattctg atagcttttg gttcatattt taaaagctga
      421 cgaacaattt cacttccaat ggaaccagtt ccgccagtta ctaatattgt ttttccttta
      481 aacacttata tctcctccta tgaattatag tcatctataa ttcgattctt aatctttaaa
      541 aaatatataa ctaaactaat tatcaataat gatgtcaaaa c
//
LOCUS       DAZKOK010000294          573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000294 DAZKOK010000000
VERSION     DAZKOK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.308"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>573
                     /locus_tag="ACG90W_002992"
     CDS             <1..>573
                     /locus_tag="ACG90W_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0479263.1"
                     /translation="GAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGP
                     AAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLIT
                     MIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIG
                     TVASAIVNILLNMIFIPRLGIIAAGYTTVF"
BASE COUNT          181 a           78 c          106 g          208 t
ORIGIN      
        1 agggtgctca tttttttgat aaaaaacagt ggagatacgc tttaaaaatc tctttgccgc
       61 taatcccaca ttccttagca aatatagttt tagcacaatt cgatagaatt atgattaatt
      121 cttatttagg tcctgctgcg gggggaatat attcctacat tagtaacata ggtattattc
      181 taagcgtaat atggatgtct actaataacg cctgggttcc atggttctat ggtgagatga
      241 gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta gtctttactt
      301 tgattacgat gattgtaatg gtagtttcga ttgatttagc aaaatttatg gctccgaaag
      361 actattattc tgggttatct ttagtggttc cgataacctt aggctatttc tttcagtttt
      421 tatatagttt accagttaat gctgaatttt atgagaaaaa aacatcgttt attgcgattg
      481 gtacagtagc aagtgctata gttaatatac tattaaacat gattttcata cctaggttag
      541 gaataattgc tgctggatac acaaccgtat tta
//
LOCUS       DAZKOK010000295          569 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000295 DAZKOK010000000
VERSION     DAZKOK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.309"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..341)
                     /locus_tag="ACG90W_002993"
     CDS             complement(111..341)
                     /locus_tag="ACG90W_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479264.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          198 a           87 c           68 g          216 t
ORIGIN      
        1 gattgctaaa aagaaaactt ctgaatccca caccttataa aaatctttga caaatctatt
       61 cgaaggatca aatttaaacc ataattgcgt ctcattaaaa agcataacca ttactccgat
      121 ctatttttaa acttagtctc tacctctaat atatatcgag tttttatttt gccttcagag
      181 aatactgttt ctttttttgc agttacaggt tgtttatttt cggaaaaagc taatatagct
      241 aaaattgaaa catccatctg gaatttatct tttttactgc tttgatcata aaagtctgca
      301 tattcatcac tgatattttt tctaataaat tcttccatca taaaaatcac ctcaaaacga
      361 ttatacgaac ttacgttctt gttgtaaagc gatatttgag gggcaaaaaa ggggcataat
      421 ttgactgata ttaagttagc tattgatata tgaatgacat acaaaaatat aaaatctttt
      481 aaaaatgctt atatatcaat gtttttgcca ttcttttata tatctgttat atgtcaaaaa
      541 aatgtgttca atttcaattt gtatttgaa
//
LOCUS       DAZKOK010000296          567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000296 DAZKOK010000000
VERSION     DAZKOK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.310"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..418
                     /locus_tag="ACG90W_002994"
     CDS             <1..418
                     /locus_tag="ACG90W_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0479265.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
BASE COUNT          227 a           78 c          102 g          160 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgct
//
LOCUS       DAZKOK010000297          555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000297 DAZKOK010000000
VERSION     DAZKOK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.311"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(157..456)
                     /locus_tag="ACG90W_002995"
     CDS             complement(157..456)
                     /locus_tag="ACG90W_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0479266.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          178 a          100 c           67 g          210 t
ORIGIN      
        1 tgtttttgtg ttaattaaag gataaaatac aatataaaga aacatctgtt tctttattta
       61 gaataaaaaa atacagactc tgttcaaata aatgaaccta gcctgcatta tgagattgac
      121 tatacttttt aattcattga gatgaataac tcgtttttat aaatgcccaa aagtaatcgg
      181 tgccgaagcc attcctcgac ctaattcttt ttcaaatttt cctattgttg atatctccat
      241 ataaaacggt gatactggtt tagacaattc tcccctgatt gctaacggac gtaaactacg
      301 ttccaaatta tttatgactt taggataata ctgacctttt tctaagcctg tctttgcgtc
      361 tatcaaaagt atacgttcct tttctttgat ctctggattt aaaacgagat catataattt
      421 tagtaccatg ttttctattt cttcactttt agacatcacg atttcctccc aaaaatatat
      481 ttcttcccac tacattctat aaacaaagta tacgctacat ctagattttt ttaaatcatt
      541 actggattgt taaaa
//
LOCUS       DAZKOK010000298          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000298 DAZKOK010000000
VERSION     DAZKOK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.312"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG90W_002996"
     CDS             <1..354
                     /locus_tag="ACG90W_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0479267.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZKOK010000299          524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000299 DAZKOK010000000
VERSION     DAZKOK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.313"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..350)
                     /locus_tag="ACG90W_002997"
     CDS             complement(48..350)
                     /locus_tag="ACG90W_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0479268.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          177 a           92 c           74 g          181 t
ORIGIN      
        1 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
       61 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      121 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      181 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      301 ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat aatgttcacc
      361 tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat tgataatatg
      421 gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg ccattttcat
      481 tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac cctt
//
LOCUS       DAZKOK010000300          521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000300 DAZKOK010000000
VERSION     DAZKOK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.314"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..>521
                     /locus_tag="ACG90W_002998"
     CDS             25..>521
                     /locus_tag="ACG90W_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0479269.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          190 a           95 c           86 g          150 t
ORIGIN      
        1 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
       61 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      121 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      181 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      241 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
      301 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
      361 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
      421 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
      481 ttaaatgata agtatgtcgc tatttttatg gacgctactt a
//
LOCUS       DAZKOK010000301          514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000301 DAZKOK010000000
VERSION     DAZKOK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.315"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG90W_002999"
     CDS             complement(<1..132)
                     /locus_tag="ACG90W_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0479270.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZKOK010000302          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000302 DAZKOK010000000
VERSION     DAZKOK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.317"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            198..>512
                     /locus_tag="ACG90W_003000"
     CDS             198..>512
                     /locus_tag="ACG90W_003000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0479271.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTN"
BASE COUNT          199 a           58 c           84 g          171 t
ORIGIN      
        1 taaaatagta atatagctga tttggattca attcatattt atttataaat caaaaataca
       61 gagaatagta tataaatgtc ctttcctatt cttaaaatga tgtttgtact tacttaaatt
      121 aggtataatt agtatatgtt ttgtgagaaa tttagactta caataagcag tgaatttata
      181 atcaacgtat aggagacatg gatgaaatga aactaattgg tatagattta gacggtactc
      241 ttttgaattc tgaccagagc attagtcaaa aaaatgcaag aacaatgaaa catttaccgg
      301 aagactgttt tccattcatt tgttctggaa gagaagtaga agacattaat aatattctca
      361 aaaaatctgg tttaaggata ccagcagtag ggttaaatgg ttctgttgga tttgatggag
      421 aaaaaaagat atttgagtct tccttcgata aaaatgatgt aaaaaaaatt gattctgtcc
      481 tatcgaaatt tccaaccaaa atatatacaa at
//
LOCUS       DAZKOK010000303          497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000303 DAZKOK010000000
VERSION     DAZKOK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.319"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..>497)
                     /locus_tag="ACG90W_003001"
     CDS             complement(119..>497)
                     /locus_tag="ACG90W_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFQ0479272.1"
                     /translation="EKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIE
                     IVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKK
                     ADYVTVSNDKDGVSQAFERILTI"
BASE COUNT          157 a           86 c           65 g          189 t
ORIGIN      
        1 tcctaaatat ctatgttata ttcgcttcgt ttgacactga agaagtacta tttgtactgg
       61 aatttatgct ccatttttcc gtatttagaa ttctatgttc tatactacct taattagttt
      121 atatggtaag aattctttca aatgcttgag atactccgtc tttatcattt gaaacagtga
      181 cataatcagc ttttttcatg atttcttgat gactatttcc cattgcaaaa ctaatatctg
      241 cactttcaaa catagataag tcatttaaac tatctccgat cgcgattctc aaaggtttct
      301 ttaaattgta gattgattcc aaacgatcaa acaccacacc tttagataca ttttgaggta
      361 cgatttcaat atttacaggt gccgattcag taacagacac atttagcatc tttttcaaat
      421 ggttaaatat attagctttc aattgacgat taggaataaa aataaaaaat ttgtaaatgc
      481 tgatagcatc cttttct
//
LOCUS       DAZKOK010000304          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000304 DAZKOK010000000
VERSION     DAZKOK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.320"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACG90W_003002"
     CDS             43..165
                     /locus_tag="ACG90W_003002"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479273.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            138..269
                     /locus_tag="ACG90W_003003"
     CDS             138..269
                     /locus_tag="ACG90W_003003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479274.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DAZKOK010000305          492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000305 DAZKOK010000000
VERSION     DAZKOK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.321"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>492
                     /locus_tag="ACG90W_003004"
     CDS             <1..>492
                     /locus_tag="ACG90W_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0479275.1"
                     /translation="GWTPDVIIGRKERSVSCGMRTLYRLFSKGIFDIDTLPMKGKRKP
                     NGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVI
                     ITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFAD
                     PGTP"
BASE COUNT          159 a           94 c           93 g          146 t
ORIGIN      
        1 tggttggacg cctgacgtca ttatcgggcg aaaagaaagg tctgtttcat gcggtatgag
       61 aacactttat cgtttatttt ctaaaggaat atttgatatt gacacactac cgatgaaagg
      121 taaaagaaaa cccaatggcc atcaggaaaa acggggaaaa caacagtatc agcgctcaat
      181 ccatgatcga cctgataatt atcctgattt caattctgag tttggtcacc ttgaaggtga
      241 tacgatcgtt ggcattcatc ataaaagtgc cgtcattact ttagttgaaa gattatctaa
      301 agtcattatc acgattaaac ccaacggccg taaggcatta gatattgaaa ctgcccttaa
      361 tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct attacgtttg actgtggaaa
      421 agaattttct aactggaaag ccattagtaa ccaacatgat attgatatat attttgcgga
      481 ccctggaaca cc
//
LOCUS       DAZKOK010000306          471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000306 DAZKOK010000000
VERSION     DAZKOK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.322"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          153 a           91 c           56 g          171 t
ORIGIN      
        1 acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag atgaaagatc
       61 aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact actttttcct
      121 ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct ttcaatgctt
      181 gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc tagaacatat
      241 tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa tttcgatagg
      301 ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc aagataggaa
      361 tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt cacgctccac
      421 aaaattgttt tatacactac tattcagccc attcaacgac caatgaattt t
//
LOCUS       DAZKOK010000307          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000307 DAZKOK010000000
VERSION     DAZKOK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.323"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG90W_003005"
     CDS             <1..>468
                     /locus_tag="ACG90W_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0479276.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKOK010000308          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000308 DAZKOK010000000
VERSION     DAZKOK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.324"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG90W_003006"
     tRNA            complement(20..91)
                     /locus_tag="ACG90W_003006"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG90W_003007"
     tRNA            complement(113..200)
                     /locus_tag="ACG90W_003007"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG90W_003008"
     tRNA            complement(225..298)
                     /locus_tag="ACG90W_003008"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG90W_003009"
     tRNA            complement(312..382)
                     /locus_tag="ACG90W_003009"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG90W_003010"
     tRNA            complement(384..459)
                     /locus_tag="ACG90W_003010"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKOK010000309          454 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000309 DAZKOK010000000
VERSION     DAZKOK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.325"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..378)
                     /locus_tag="ACG90W_003011"
     CDS             complement(<1..378)
                     /locus_tag="ACG90W_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479277.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDS"
BASE COUNT          129 a           78 c           72 g          175 t
ORIGIN      
        1 actatcaatc aaagcaactg taccattttg attattcgtt gtataactct gaacaggatt
       61 tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat tcttgtatgc
      121 tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt tcaaaaacgg
      181 aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag aatcattttt
      241 aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct tacggacaca
      301 accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc tgaatttgta
      361 tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt ccttgatttt
      421 ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAZKOK010000310          441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000310 DAZKOK010000000
VERSION     DAZKOK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.326"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>441
                     /locus_tag="ACG90W_003012"
     CDS             <1..>441
                     /locus_tag="ACG90W_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0479278.1"
                     /translation="TTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDT
                     EFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVD
                     VSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          179 a           67 c          101 g           94 t
ORIGIN      
        1 aacaacagtc tttgcagtga aagacggcgt aagcttacca gaagataaac ttccagtgtt
       61 gaaagcaaaa gatggttata cagatgcgaa atggccagag gaagcaactc aaccaattac
      121 agcagatgat acagaatttg tatcaagtgc aacaaaactg gatgataaat ctgacgctga
      181 caaatataat cctgaaggtc aaaaagtgac tacagaattg aataaggaac cagacgcatt
      241 tgagggaatt aagaacaaag aagatttacc aaaagatact aagtatactt ggaaagaaaa
      301 agtagatgtt agcgcagctg gaaataaaaa agggactgtt gtagtgacat atccagatgg
      361 atcatctgat gaagttgaag tagatgttac agtgacagac aatcgttctg acgccgataa
      421 atatgagcca acagtagaag g
//
LOCUS       DAZKOK010000311          436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000311 DAZKOK010000000
VERSION     DAZKOK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.327"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          134 a           67 c           80 g          155 t
ORIGIN      
        1 tatgaaaaca atcacaaacg taggttttaa agtcataatc gttagatatt ttagttattt
       61 ttcattcaaa aaaataggga actagtgttt gcatatgtgc ttttttgagt tataataatt
      121 catgaagaga ggtaattcac ttatttcccc aagctgaaaa gcctgatatt acttcttttc
      181 aggcagtgct gctaatattc aattacactt ttttgtatat ttttagttgt tttaaattta
      241 attgtctgat atgcttgtga tgaattgatt ttcgttctgc acttttggac gataaattgt
      301 gataattgaa aatacaaaaa aaagaaaggc ccctgtaagg ctcgggagac tggtaatctc
      361 cgtcagtgtc ctacaggaaa gcattaagat gacgccttaa tgcgtgccct tcagtgttac
      421 ggtattaccc gcaacg
//
LOCUS       DAZKOK010000312          435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000312 DAZKOK010000000
VERSION     DAZKOK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.328"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>435
                     /locus_tag="ACG90W_003013"
     CDS             22..>435
                     /locus_tag="ACG90W_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0479279.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRG"
BASE COUNT          156 a           82 c           89 g          108 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtgga
//
LOCUS       DAZKOK010000313          429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000313 DAZKOK010000000
VERSION     DAZKOK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.329"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..387
                     /gene="ssrA"
                     /locus_tag="ACG90W_003014"
     tmRNA           21..387
                     /gene="ssrA"
                     /locus_tag="ACG90W_003014"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          119 a           90 c           97 g          123 t
ORIGIN      
        1 cgtaaccctt gttagctaac ggggacgtta cggattcgac aggcacagtc gagcttgaat
       61 tgcgtttcgt aggttacgtc tacgtaaaaa cgttacagtt aaatataact gctaaaaacg
      121 aaaacaactc ttacgcttta gctgcctaaa aacagttagc gtagatcctc tcggcatcgc
      181 ccatgtgctc gagtaagggt cctaacttta gtgggatacg tttcaacttt ccgtctgtaa
      241 gttgaaaaag agaacatcag actagcgata cagaatgcct gtcactcggc aagctgtaaa
      301 gtgaatcctt aaatgagttg actatgaacg tagattttta agtggcgatg tgtttggacg
      361 cgggttcgac tcccgccgtc tccattgtga gatttcagca gatttcgcta gatgcttaaa
      421 tccttattt
//
LOCUS       DAZKOK010000314          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000314 DAZKOK010000000
VERSION     DAZKOK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.330"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          177 a           48 c           59 g          137 t
ORIGIN      
        1 agtgtgtata cgaaataaac aaaaaaatct taagtaaatt tctagatacc taatttaatc
       61 ctaactagtg tatcctaaag ttaataacga gtttatcaca atgaaaatta taaaatgctc
      121 ccatattaga tataacatgc aatatttgaa ttagaactac aaaatttatt cgttttataa
      181 agtaaataat gttgagatac taaggaatag aatattataa ggaattgagc tgaatgtatt
      241 ggataaaaaa agaataactt tattagagat ggactataaa aatgctcgta attttcttct
      301 gaagtctgaa agctatttga atgctcagct accagaatat attagttttg acgaaacttt
      361 aaaatctgct aaaaaagttt taacttcgcc gtttgtataa ttaagcgaga caaataaaaa
      421 a
//
LOCUS       DAZKOK010000315          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000315 DAZKOK010000000
VERSION     DAZKOK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.331"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG90W_003015"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG90W_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKOK010000316          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000316 DAZKOK010000000
VERSION     DAZKOK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.332"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..403
                     /locus_tag="ACG90W_003016"
     CDS             74..403
                     /locus_tag="ACG90W_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0479280.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DAZKOK010000317          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000317 DAZKOK010000000
VERSION     DAZKOK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.333"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG90W_003017"
     rRNA            <1..>411
                     /locus_tag="ACG90W_003017"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKOK010000318          396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000318 DAZKOK010000000
VERSION     DAZKOK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.334"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..385)
                     /locus_tag="ACG90W_003018"
     CDS             complement(<1..385)
                     /locus_tag="ACG90W_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479281.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDI"
BASE COUNT          138 a           59 c           67 g          132 t
ORIGIN      
        1 atatatcttt catatctaaa acttctgaaa aagcgactga gtagccagga gagattgctt
       61 gtgccaacgg tataggagat aaaaggctac ttttatgaat gcttcccata tcaaaaccat
      121 tggcgtgata atgtgaacaa ttaattttct taccgttgta aagatacgta cattcgataa
      181 ttgagtttgg catttttgat gggtttgcaa ttacgccagt aagaatcatt cgattacttc
      241 tatcatgctt aactaaaaaa taggagagcg taatttttct tttatcttta aataagttat
      301 agagggataa aaaaaatcct gaaatagaaa tgattaaagt aatagcgact tggtaatcgc
      361 tcaaaatttt tatcaagtaa ttcaccacct ttttac
//
LOCUS       DAZKOK010000319          389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000319 DAZKOK010000000
VERSION     DAZKOK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.335"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>389
                     /locus_tag="ACG90W_003019"
     CDS             <1..>389
                     /locus_tag="ACG90W_003019"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFQ0479282.1"
                     /translation="ANKTVDDEEAIKILQEVEGIGTEATRASIIEALKQKEYIQVIKN
                     KLVVTEKGKLLCQAVESQHLLTSAEMTAKWETYLKKIGKREGNQENFITNIKKFIVHL
                     LEAVPNDIEKLNFSDYQEQKEKEAEKS"
BASE COUNT          169 a           47 c           83 g           90 t
ORIGIN      
        1 cggcgaataa aacggttgat gatgaagaag caatcaagat tttacaagaa gttgagggga
       61 ttggaacaga agcgacaaga gcaagtatta ttgaagcgtt aaaacaaaaa gaatatatcc
      121 aagtgattaa gaataagctt gttgtaactg aaaaaggaaa attattgtgc caggcagttg
      181 aaagtcagca ccttttaacg agtgctgaaa tgacggctaa atgggaaacg tatttaaaaa
      241 aaatcggtaa aagagaaggc aatcaagaga actttattac gaatatcaaa aaattcattg
      301 ttcatttact ggaagctgta cctaacgata tagaaaaact aaatttttct gattaccagg
      361 aacagaaaga aaaagaagca gaaaaaagt
//
LOCUS       DAZKOK010000320          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000320 DAZKOK010000000
VERSION     DAZKOK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.336"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG90W_003020"
     CDS             <1..>386
                     /locus_tag="ACG90W_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0479283.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKOK010000321          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000321 DAZKOK010000000
VERSION     DAZKOK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.337"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG90W_003021"
     CDS             <1..>386
                     /locus_tag="ACG90W_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0479284.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKOK010000322          381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000322 DAZKOK010000000
VERSION     DAZKOK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.339"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..378)
                     /locus_tag="ACG90W_003022"
     CDS             complement(<1..378)
                     /locus_tag="ACG90W_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479285.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLI"
BASE COUNT          164 a           47 c           55 g          115 t
ORIGIN      
        1 gataagtgat aataaaaaaa taccatttat aactaaacat aaactaatgg atttgggcgg
       61 tttcaggctc atcagaaaaa tatagattct ggtgaacaac aaccataaaa aagtgagaat
      121 cattgcatga gagcttcttt ccataaattt tcctttaaga gtagtgctta aaagtacaga
      181 aaaaataata aatgcaagga taggagcaat taaagtaaat ttatttaata ataaacaaaa
      241 tttttcagct tttttattct tggaataacg aggtattgtt ttcgataaaa aaagatggtg
      301 cagacaaatt atgataatta agcatactat cagataactc aaaatgaagc cttctttcaa
      361 aaaattattc aaaaccatat a
//
LOCUS       DAZKOK010000323          381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000323 DAZKOK010000000
VERSION     DAZKOK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.340"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACG90W_003023"
     tRNA            complement(21..94)
                     /locus_tag="ACG90W_003023"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACG90W_003024"
     tRNA            complement(108..181)
                     /locus_tag="ACG90W_003024"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACG90W_003025"
     tRNA            complement(209..281)
                     /locus_tag="ACG90W_003025"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACG90W_003026"
     tRNA            complement(301..374)
                     /locus_tag="ACG90W_003026"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAZKOK010000324          372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000324 DAZKOK010000000
VERSION     DAZKOK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.341"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          163 a           50 c           62 g           97 t
ORIGIN      
        1 aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat gacatgataa
       61 gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga aaaaaacttc
      121 aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc tgtaagattt
      181 cgaaaaacga aatcaaaaaa acttttaaaa aagagttgac aattacttgt ctctctgata
      241 tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg atgaagtaga
      301 cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg attcaattca
      361 agacaacaaa ca
//
LOCUS       DAZKOK010000325          368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000325 DAZKOK010000000
VERSION     DAZKOK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.342"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG90W_003027"
     CDS             complement(<1..272)
                     /locus_tag="ACG90W_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479286.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          106 a           69 c           52 g          141 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcataa
//
LOCUS       DAZKOK010000326          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000326 DAZKOK010000000
VERSION     DAZKOK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.343"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG90W_003028"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG90W_003028"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKOK010000327          359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000327 DAZKOK010000000
VERSION     DAZKOK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.344"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..258
                     /locus_tag="ACG90W_003029"
     CDS             19..258
                     /locus_tag="ACG90W_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0479287.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          149 a           38 c           79 g           93 t
ORIGIN      
        1 agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaaga aaaacgaaat
       61 gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg tgttcgtgct
      121 gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt aaaagaagtt
      181 atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcatta tcttaaaatt
      241 ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga
      301 ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt tttgaaaca
//
LOCUS       DAZKOK010000328          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000328 DAZKOK010000000
VERSION     DAZKOK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.346"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG90W_003030"
     CDS             <1..>344
                     /locus_tag="ACG90W_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0479288.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKOK010000329          331 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000329 DAZKOK010000000
VERSION     DAZKOK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.347"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACG90W_003031"
     CDS             complement(<1..208)
                     /locus_tag="ACG90W_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479289.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
BASE COUNT          131 a           61 c           29 g          110 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat t
//
LOCUS       DAZKOK010000330          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000330 DAZKOK010000000
VERSION     DAZKOK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.348"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG90W_003032"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG90W_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0479290.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZKOK010000331          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000331 DAZKOK010000000
VERSION     DAZKOK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.349"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG90W_003033"
     CDS             <1..>321
                     /locus_tag="ACG90W_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0479291.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKOK010000332          315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000332 DAZKOK010000000
VERSION     DAZKOK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.351"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>315
                     /locus_tag="ACG90W_003034"
     CDS             <1..>315
                     /locus_tag="ACG90W_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479292.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DAZKOK010000333          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000333 DAZKOK010000000
VERSION     DAZKOK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.352"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..>309
                     /locus_tag="ACG90W_003035"
     CDS             10..>309
                     /locus_tag="ACG90W_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0479293.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT          110 a           34 c           56 g          109 t
ORIGIN      
        1 agtataaaaa tgaacaaaca taatagaaaa ataacgaata acaaagaact gatcttgtta
       61 ctaataggga tttggggtat ctttttactc tttaatatgt atatgccgac tatgagagcg
      121 gatgacgtag tttatgcaag tagattagat gaacttggct atttgggtgc ttcgatagaa
      181 cattataaga catggagttc aagaattatt atagaactat tcttaatgtt cttttctaaa
      241 cactttatgc tatggaaatt attaaattct acgattatgt taggaattgt agtattactt
      301 tgcaagtat
//
LOCUS       DAZKOK010000334          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000334 DAZKOK010000000
VERSION     DAZKOK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.354"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..301
                     /locus_tag="ACG90W_003036"
                     /pseudo
     CDS             1..301
                     /locus_tag="ACG90W_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           60 c           57 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 g
//
LOCUS       DAZKOK010000335          299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000335 DAZKOK010000000
VERSION     DAZKOK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.355"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>299
                     /locus_tag="ACG90W_003037"
     CDS             <1..>299
                     /locus_tag="ACG90W_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0479294.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZKOK010000336          280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000336 DAZKOK010000000
VERSION     DAZKOK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.356"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>280
                     /locus_tag="ACG90W_003038"
     CDS             82..>280
                     /locus_tag="ACG90W_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0479295.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGK"
BASE COUNT          101 a           40 c           59 g           80 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga
//
LOCUS       DAZKOK010000337          260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000337 DAZKOK010000000
VERSION     DAZKOK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.357"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..194
                     /locus_tag="ACG90W_003039"
     CDS             <1..194
                     /locus_tag="ACG90W_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0479296.1"
                     /translation="RITPHGFRHTHASLLLEAGESVKVVQQRLGHENSKVTLDIYAHI
                     TNNAPKKTGQDFADMMAHQ"
BASE COUNT           98 a           45 c           48 g           69 t
ORIGIN      
        1 ctcgtattac acctcatggg tttagacata cacatgctag tttattatta gaagcaggag
       61 aatctgtcaa ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca
      121 tctatgcaca tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga
      181 tggctcatca gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa
      241 aaaagctaga acccttatat
//
LOCUS       DAZKOK010000338          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000338 DAZKOK010000000
VERSION     DAZKOK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.358"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..239
                     /locus_tag="ACG90W_003040"
     CDS             <1..239
                     /locus_tag="ACG90W_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFQ0479297.1"
                     /translation="GNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGK
                     ETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
BASE COUNT          109 a           32 c           43 g           68 t
ORIGIN      
        1 gtggcaacaa tattgtatta aaaaaatcgt tttatggttg ttcaaattat cctgaatgta
       61 agtttacttt agctgaacat tttagaaaga aaaaactaac caaaacgaat gtaaaagaat
      121 tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa aagccctaca
      181 atgccgttgt aaaaattggg gaaaagggat atattgattt tatctctttt tcaaaataaa
      241 cataaaagcc ct
//
LOCUS       DAZKOK010000339          239 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000339 DAZKOK010000000
VERSION     DAZKOK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.360"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..222
                     /locus_tag="ACG90W_003041"
     CDS             <1..222
                     /locus_tag="ACG90W_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0479298.1"
                     /translation="LKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRV
                     AYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT           84 a           24 c           51 g           80 t
ORIGIN      
        1 ttgaaagaaa aatttttgaa tttaatttat gatgaaattg cgattgatcc atataagggt
       61 gaacaaaaaa ctggggattt atcaggtatt tggtctagtg ggttcaaata tgcgggcaca
      121 acatatagag tagcttacga gattatagaa aacaaggtta ttccagtatt gttatgtgga
      181 acgcatgaaa acttttatga acagttgaaa aaattaatct agtgatttgt tgtcgttct
//
LOCUS       DAZKOK010000340          233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000340 DAZKOK010000000
VERSION     DAZKOK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.361"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZKOK010000341          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000341 DAZKOK010000000
VERSION     DAZKOK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.362"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>232)
                     /locus_tag="ACG90W_003042"
     CDS             complement(<1..>232)
                     /locus_tag="ACG90W_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479299.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DAZKOK010000342          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000342 DAZKOK010000000
VERSION     DAZKOK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.363"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG90W_003043"
     CDS             <1..>224
                     /locus_tag="ACG90W_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0479300.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKOK010000343          222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000343 DAZKOK010000000
VERSION     DAZKOK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.364"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>222
                     /locus_tag="ACG90W_003044"
     CDS             <1..>222
                     /locus_tag="ACG90W_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0479301.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT           67 a           40 c           45 g           70 t
ORIGIN      
        1 tatcttttat tgcgaaaaat tgtcaggtat ctttgaccac cgttattcgt acgttgaaag
       61 agtttaaaag ctacttacca aagcaatcta agaggattct ccccagagta ttgatggttg
      121 atgaatttcg ttcgcacgct tccatagaag ataaaatgag ctttatttgc gcagatggcg
      181 aaacaggaca attaattgat gtcttgccta cgcgtaaatt gc
//
LOCUS       DAZKOK010000344          220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000344 DAZKOK010000000
VERSION     DAZKOK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.denovo.365"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG90W_003045"
     rRNA            complement(<1..175)
                     /locus_tag="ACG90W_003045"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAZKOK010000345        10344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.101, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000345 DAZKOK010000000
VERSION     DAZKOK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.101"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..986
                     /locus_tag="ACG90W_003046"
     CDS             90..986
                     /locus_tag="ACG90W_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0479302.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1136..2077)
                     /locus_tag="ACG90W_003047"
     CDS             complement(1136..2077)
                     /locus_tag="ACG90W_003047"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0479303.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2203..3324
                     /locus_tag="ACG90W_003048"
     CDS             2203..3324
                     /locus_tag="ACG90W_003048"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0479304.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3582..5084
                     /gene="eat(A)"
                     /locus_tag="ACG90W_003049"
     CDS             3582..5084
                     /gene="eat(A)"
                     /locus_tag="ACG90W_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0479305.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5151..5678
                     /locus_tag="ACG90W_003050"
     CDS             5151..5678
                     /locus_tag="ACG90W_003050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0479306.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5889..6950
                     /locus_tag="ACG90W_003051"
     CDS             5889..6950
                     /locus_tag="ACG90W_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0479307.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6953..7849
                     /gene="murQ"
                     /locus_tag="ACG90W_003052"
     CDS             6953..7849
                     /gene="murQ"
                     /locus_tag="ACG90W_003052"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0479308.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7863..9845
                     /locus_tag="ACG90W_003053"
     CDS             7863..9845
                     /locus_tag="ACG90W_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0479309.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9910..>10344)
                     /gene="nrdI"
                     /locus_tag="ACG90W_003054"
     CDS             complement(9910..>10344)
                     /gene="nrdI"
                     /locus_tag="ACG90W_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0479310.1"
                     /translation="YISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHENSDFT
                     KETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNN
                     QYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3381 a         1834 c         2247 g         2882 t
ORIGIN      
        1 catacaaata taggaaacgt tttactggct ttttactatt taaacaggct atcataggaa
       61 aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa
      121 aaaataaatc actttatttt ttgccttcca tccgctcttt agatgtttgg tccggaaaag
      181 atttactaca gtcggaattc attaaatcag ctagacaaca gttggcaaat tatcagctga
      241 tcgttttttt agattatgga tttgatatgg agatggctcg acgcgtgcgc ccatttacca
      301 atgcaaaaat tgtgttattt ttttggaatc acttcaaagc agaacacgag gcgatgctga
      361 agcaggcaca agatgaaccg gcaatcgatg agatttatca ttttgatttt ttggaagcaa
      421 aagaacttgg gctgaaacat aatagttcat tttattcgaa acatatgaat ttttcttcta
      481 gcgaaccaaa gacggatcta ttttttggtg caacgaataa tgggagaaaa gaacgagcag
      541 aaagctataa gcaggaattt ataaaaagag atgttacgac caactatttc attcttccac
      601 atcgagggaa tgaacaacct ggatatctta cttatgaaga gtacctgaaa aaaacagggg
      661 aatcgcgcgg gattctggaa ctgttgcggg agggtcagca aggagtgact ttgcggacat
      721 ttgaatccat gtattttcaa aagaaactag tgacggataa tcaagcgatt gctcattatc
      781 atttttatcg tcctgaaaat atttttttgc ttcgagaacg tgatttagat gaattaccgg
      841 aatttctgca tacaccattt aagccaatcc tatcagagct attggaattt tatgatgcag
      901 aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg
      961 acctctcctc tgttgaaaac aaataaaaaa cctacatttt gaagagatta tgtactgaaa
     1021 caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga tcttttattg
     1081 ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt
     1141 ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg
     1201 tagcaatatc atatttttgt ccttttcgcc aaatggcacc attgatgtag tcgatttcag
     1261 tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt
     1321 caggatcata agtagaagca atatgttggt acacttcttc ttgatctagt atgattcctt
     1381 cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgctt
     1441 tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc
     1501 catttacaca agcttttcgc caaatggaat aacgaacgtt atggctatag atcggattta
     1561 aatttgcttc ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca
     1621 agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac
     1681 cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta
     1741 catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg
     1801 attggatcga acaaaacata tcatctaatt gattggcttt tgttaatgct acgatcaagt
     1861 ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca
     1921 cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag
     1981 gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc
     2041 tgcccattgc tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat
     2101 gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa
     2161 tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca
     2221 attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc
     2281 tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact
     2341 gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc
     2401 ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca
     2461 gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact
     2521 atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa
     2581 gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta
     2641 ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact
     2701 aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct
     2761 gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa
     2821 aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag
     2881 caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc
     2941 ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc
     3001 agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa
     3061 gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca
     3121 aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga
     3181 ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa
     3241 gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga
     3301 tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc
     3361 aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg
     3421 cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg
     3481 gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt
     3541 tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa
     3601 aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa
     3661 attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt
     3721 tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat
     3781 ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg
     3841 atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc
     3901 ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac
     3961 tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt
     4021 tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca
     4081 tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg
     4141 aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac
     4201 ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa
     4261 agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga
     4321 aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg
     4381 atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca
     4441 gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc
     4501 aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc
     4561 tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc
     4621 ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg
     4681 ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg
     4741 aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat
     4801 ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc
     4861 agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg
     4921 aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag
     4981 aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc
     5041 tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag
     5101 aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg
     5161 taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg
     5221 tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt
     5281 tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac
     5341 aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg
     5401 attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag
     5461 cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc
     5521 ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg
     5581 ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat
     5641 tcatggtaga taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa
     5701 taaaatattt caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct
     5761 tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga
     5821 aattttgttt ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga
     5881 gggataatat gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac
     5941 agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac
     6001 cagaagatga tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa
     6061 agaataaact agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt
     6121 cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc
     6181 atatttccaa tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta
     6241 gcacgatcac gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga
     6301 ttgaagcttg gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc
     6361 agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag
     6421 atacagagtt gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga
     6481 tccatcctct tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg
     6541 gagatgcagg tctgtcagaa aacgttcagg agcaatttgc ttctttccaa aaagaacaga
     6601 cagtcttgct ccatacggaa cctgtagatg aagaatttta cgaatatata ttagggaagc
     6661 atacgaatcg gcaagacgat gcaagagatg tcatacgaag cgtagatgca cgttttcgag
     6721 agattccgcc tatcccagca agaaatacag ctacgcgtat gaaagggtct attactttag
     6781 ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aaccaaatat gatttgccag
     6841 cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga agagctacca ttgatcaacc
     6901 aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa
     6961 aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga
     7021 gatgagtatt tctcaagccg ttcaactaat gaacaaagaa gatcagaaag tggccgctgc
     7081 ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct aacacgattg agtcctttaa
     7141 aaaaggtgga cgtctgattt atatgggagc cggaacaagt ggaagactag gtgtgttaga
     7201 tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa atggtcatag gtctaattgc
     7261 tggtggagaa gaagcaatga ctgtagctgt cgaaggggcc gaagattctt atgagctagg
     7321 gcaagaagat ctaatcaagc tgaatttgac aaaaaatgat atagttattg gtatcgcagc
     7381 tagcggtcgt acaccatatg tgatcggtgg attagattac gcaaaatcga tcggtgctgt
     7441 cacaggaacg atttcttgca ataaacaagc cgaaatcagt aaacacgcgg atttcccaat
     7501 cgaagtcgat gcaggtccag aatttttgac aggctctact cgattgaagt caggaacagc
     7561 acaaaaactt atcttgaata tgatttctac tatcagtatg attggtatag gtaaagtatt
     7621 caataacttg atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg
     7681 aatcatcatg caagcgacag aagctgatta cgaaacagct gaaaagtact ttgaagaagc
     7741 agatcaaaat gttaagctgg ctatcgtcat gattttgaca aatagtgata aagaggaagc
     7801 tcaagcaaaa ttagctgaag caaacggatt tgtaaaaggg accttatgag ggggaaaaga
     7861 ccatggcaaa aaacgaactg actttggaag aactggcaaa acaaatttat aaaggggcag
     7921 gcggcatggg caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt atgaagatca
     7981 tcaatgatga tcttgtaaat gtctctgaat taaaagcgat tccaggagtg cttggtgtag
     8041 tagaagatga acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc
     8101 aaatggttga tttggcaggc gtgaaattag gagaaaaact ttcttctgta agtacagcta
     8161 cagcaaattc atcagcatct ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag
     8221 cggcacaaaa atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa gacatttcta
     8281 atatctttgt gccaatgatt cctgcatttg tcggtactgg gatcgtggca gggattgccg
     8341 cagttttagc aaacttagtc acagccggaa cacttgatgc agcaacatgg caacaatata
     8401 tcgatgtgat gaacatcttg aaaaacggga tgtttagcta cctagttatc tatacaggta
     8461 tcaatgctgc acaagttttt ggtgcaacac caactcttgg tggagttatc ggggccgttg
     8521 tgatgctgac agggatgaat ccggaagcac cactgaaaaa cttatttact ggtgaagctc
     8581 ttgcagctgg acaaggtggg atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg
     8641 ttgagaaaaa actgcataaa gtagtacctg atgcggtaga tatcatcgtt acaccaactc
     8701 tttcattgat tgccatcggt ctgattgaaa ttttcttcat catgccttta gcaggtttca
     8761 tttctgatgg catggtcggt gggatcaact gggtacttgg cgttggtgga gcatttgccg
     8821 gcttcgttct aggcacatta ttcttgccga tggttatgtt cggactgcat caaattttga
     8881 ctccaatcca tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt ccaattcttg
     8941 cgatggctgg tgcaggacaa gtgggtgcag cattggcatt atggattcgc tgcaaaaaag
     9001 ataaagaatt aacagaaatg atcaaaggag ctttaccagt cgggatccta ggaatcggcg
     9061 aaccattgat ttacggagta acattacctc taggacgccc atttattact gcttgtatcg
     9121 gtggaggtat cggcggggca gtgatcggaa tgatcggaaa tgtcggggct atcgctattg
     9181 gaccttcagg agccgctttg atcccattga tctctgacgg taaatggtta ggctacatcc
     9241 ttggattatt ggcagcttat gctggtggct tcgttgctac attcttcttc ggtattccta
     9301 aagaccgttt agaaaaagaa gaattagcga ctgaaacagc cacagtaaat gaagtgcctg
     9361 aaaaagtagt acctcaaact tcagctgaaa gtgtgactat cctctcatca gttgctgacg
     9421 gaacagttgt ttctttagat aaagcaagtg atccagtttt tgcacaaaag atgatgggtg
     9481 aaggctactt tgtagaacca gaaaacggac atatctattc tccagttact ggggtagtca
     9541 gctctgtctt cccaacgaaa cacgctattg ggatcacaac gccaaatggt ttagaaatct
     9601 tgcttcatat gggaatcaac acagttgact taggcggtac gccatttgat gtgaaagtaa
     9661 cagaagggca gcaagtgaca cctgatacgc ttgtagcaga agctgactta gaagcgatca
     9721 aagcagcagg caaagaaaca tccatgatcg tcttggtaac caatatggat cgtgtaaaaa
     9781 actttgtctt ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag
     9841 cttaatatga tagaaacaat agaaacgaaa gaagaaactg aaatctattt ttcagcttct
     9901 tcttttttat tatttattgt tttctgctaa aatctgataa attcgtgtca catcagatgg
     9961 tgttccccgt aattcataat ctgccaagaa aggaaagcca aatgtttgtg catattgttt
    10021 ggctgttaga caatactgat tattgaagtt tttatttccg ctaccgacta caccaagaca
    10081 aagtttatga ttgtcttcat gttccagata ttcacgcatc gtttcggtca agatttctgt
    10141 atcaccgtta tctaaaccat ttccgccgtc taaataagta ggaacgaaag taaaaaatgg
    10201 ttctgtttct ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt
    10261 gacaggatct tctgcatgca ttttttcagc atattctgct agatgtttgg caaaggcacg
    10321 cgtatttcct gaaatcgata tata
//
LOCUS       DAZKOK010000346        10344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.102, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000346 DAZKOK010000000
VERSION     DAZKOK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.102"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..986
                     /locus_tag="ACG90W_003055"
     CDS             90..986
                     /locus_tag="ACG90W_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0479311.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1136..2077)
                     /locus_tag="ACG90W_003056"
     CDS             complement(1136..2077)
                     /locus_tag="ACG90W_003056"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0479312.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2203..3324
                     /locus_tag="ACG90W_003057"
     CDS             2203..3324
                     /locus_tag="ACG90W_003057"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0479313.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3582..5084
                     /gene="eat(A)"
                     /locus_tag="ACG90W_003058"
     CDS             3582..5084
                     /gene="eat(A)"
                     /locus_tag="ACG90W_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0479314.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNLMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5151..5678
                     /locus_tag="ACG90W_003059"
     CDS             5151..5678
                     /locus_tag="ACG90W_003059"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0479315.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5889..6950
                     /locus_tag="ACG90W_003060"
     CDS             5889..6950
                     /locus_tag="ACG90W_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0479316.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6953..7849
                     /gene="murQ"
                     /locus_tag="ACG90W_003061"
     CDS             6953..7849
                     /gene="murQ"
                     /locus_tag="ACG90W_003061"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0479317.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7863..9845
                     /locus_tag="ACG90W_003062"
     CDS             7863..9845
                     /locus_tag="ACG90W_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0479318.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9910..>10344)
                     /gene="nrdI"
                     /locus_tag="ACG90W_003063"
     CDS             complement(9910..>10344)
                     /gene="nrdI"
                     /locus_tag="ACG90W_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0479319.1"
                     /translation="YISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHENSDFT
                     KETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNN
                     QYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3381 a         1834 c         2246 g         2883 t
ORIGIN      
        1 catacaaata taggaaacgt tttactggct ttttactatt taaacaggct atcataggaa
       61 aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa
      121 aaaataaatc actttatttt ttgccttcca tccgctcttt agatgtttgg tccggaaaag
      181 atttactaca gtcggaattc attaaatcag ctagacaaca gttggcaaat tatcagctga
      241 tcgttttttt agattatgga tttgatatgg agatggctcg acgcgtgcgc ccatttacca
      301 atgcaaaaat tgtgttattt ttttggaatc acttcaaagc agaacacgag gcgatgctga
      361 agcaggcaca agatgaaccg gcaatcgatg agatttatca ttttgatttt ttggaagcaa
      421 aagaacttgg gctgaaacat aatagttcat tttattcgaa acatatgaat ttttcttcta
      481 gcgaaccaaa gacggatcta ttttttggtg caacgaataa tgggagaaaa gaacgagcag
      541 aaagctataa gcaggaattt ataaaaagag atgttacgac caactatttc attcttccac
      601 atcgagggaa tgaacaacct ggatatctta cttatgaaga gtacctgaaa aaaacagggg
      661 aatcgcgcgg gattctggaa ctgttgcggg agggtcagca aggagtgact ttgcggacat
      721 ttgaatccat gtattttcaa aagaaactag tgacggataa tcaagcgatt gctcattatc
      781 atttttatcg tcctgaaaat atttttttgc ttcgagaacg tgatttagat gaattaccgg
      841 aatttctgca tacaccattt aagccaatcc tatcagagct attggaattt tatgatgcag
      901 aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg
      961 acctctcctc tgttgaaaac aaataaaaaa cctacatttt gaagagatta tgtactgaaa
     1021 caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga tcttttattg
     1081 ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt
     1141 ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg
     1201 tagcaatatc atatttttgt ccttttcgcc aaatggcacc attgatgtag tcgatttcag
     1261 tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt
     1321 caggatcata agtagaagca atatgttggt acacttcttc ttgatctagt atgattcctt
     1381 cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgctt
     1441 tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc
     1501 catttacaca agcttttcgc caaatggaat aacgaacgtt atggctatag atcggattta
     1561 aatttgcttc ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca
     1621 agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac
     1681 cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta
     1741 catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg
     1801 attggatcga acaaaacata tcatctaatt gattggcttt tgttaatgct acgatcaagt
     1861 ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca
     1921 cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag
     1981 gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc
     2041 tgcccattgc tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat
     2101 gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa
     2161 tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca
     2221 attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc
     2281 tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact
     2341 gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc
     2401 ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca
     2461 gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact
     2521 atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa
     2581 gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta
     2641 ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact
     2701 aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct
     2761 gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa
     2821 aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag
     2881 caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc
     2941 ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc
     3001 agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa
     3061 gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca
     3121 aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga
     3181 ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa
     3241 gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga
     3301 tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc
     3361 aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg
     3421 cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg
     3481 gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt
     3541 tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa
     3601 aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa
     3661 attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt
     3721 tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat
     3781 ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg
     3841 atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc
     3901 ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac
     3961 tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt
     4021 tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca
     4081 tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg
     4141 aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac
     4201 ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa
     4261 agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga
     4321 aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg
     4381 atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tctgatggag acccagatca
     4441 gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc
     4501 aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc
     4561 tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc
     4621 ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg
     4681 ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg
     4741 aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat
     4801 ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc
     4861 agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg
     4921 aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag
     4981 aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc
     5041 tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag
     5101 aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg
     5161 taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg
     5221 tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt
     5281 tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac
     5341 aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg
     5401 attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag
     5461 cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc
     5521 ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg
     5581 ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat
     5641 tcatggtaga taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa
     5701 taaaatattt caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct
     5761 tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga
     5821 aattttgttt ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga
     5881 gggataatat gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac
     5941 agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac
     6001 cagaagatga tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa
     6061 agaataaact agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt
     6121 cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc
     6181 atatttccaa tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta
     6241 gcacgatcac gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga
     6301 ttgaagcttg gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc
     6361 agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag
     6421 atacagagtt gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga
     6481 tccatcctct tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg
     6541 gagatgcagg tctgtcagaa aacgttcagg agcaatttgc ttctttccaa aaagaacaga
     6601 cagtcttgct ccatacggaa cctgtagatg aagaatttta cgaatatata ttagggaagc
     6661 atacgaatcg gcaagacgat gcaagagatg tcatacgaag cgtagatgca cgttttcgag
     6721 agattccgcc tatcccagca agaaatacag ctacgcgtat gaaagggtct attactttag
     6781 ataacgaaaa gtatttgcgc tatatgggtg agatccaatt aaccaaatat gatttgccag
     6841 cggatgaaaa agtgaatgta gtagctaaag ttatcaaaga agagctacca ttgatcaacc
     6901 aaatcaaagc tggtatgaac tatcaattta taagaaaaga aggaagataa aaatgagcaa
     6961 aatcaattta gagaacctaa caacggaacg cagaaaccaa gaaacttttg gattagatga
     7021 gatgagtatt tctcaagccg ttcaactaat gaacaaagaa gatcagaaag tggccgctgc
     7081 ggttgaaaaa gaactgacaa gtatcgaacc agtgattgct aacacgattg agtcctttaa
     7141 aaaaggtgga cgtctgattt atatgggagc cggaacaagt ggaagactag gtgtgttaga
     7201 tgctgctgaa tgcgtaccga cattcggtgt agaaccggaa atggtcatag gtctaattgc
     7261 tggtggagaa gaagcaatga ctgtagctgt cgaaggggcc gaagattctt atgagctagg
     7321 gcaagaagat ctaatcaagc tgaatttgac aaaaaatgat atagttattg gtatcgcagc
     7381 tagcggtcgt acaccatatg tgatcggtgg attagattac gcaaaatcga tcggtgctgt
     7441 cacaggaacg atttcttgca ataaacaagc cgaaatcagt aaacacgcgg atttcccaat
     7501 cgaagtcgat gcaggtccag aatttttgac aggctctact cgattgaagt caggaacagc
     7561 acaaaaactt atcttgaata tgatttctac tatcagtatg attggtatag gtaaagtatt
     7621 caataacttg atggtcgatg tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg
     7681 aatcatcatg caagcgacag aagctgatta cgaaacagct gaaaagtact ttgaagaagc
     7741 agatcaaaat gttaagctgg ctatcgtcat gattttgaca aatagtgata aagaggaagc
     7801 tcaagcaaaa ttagctgaag caaacggatt tgtaaaaggg accttatgag ggggaaaaga
     7861 ccatggcaaa aaacgaactg actttggaag aactggcaaa acaaatttat aaaggggcag
     7921 gcggcatggg caatgtagaa agcattgtcc actgcatgac tcgtgtgcgt atgaagatca
     7981 tcaatgatga tcttgtaaat gtctctgaat taaaagcgat tccaggagtg cttggtgtag
     8041 tagaagatga acagcttcaa gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc
     8101 aaatggttga tttggcaggc gtgaaattag gagaaaaact ttcttctgta agtacagcta
     8161 cagcaaattc atcagcatct ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag
     8221 cggcacaaaa atcgaaacaa aaaccatcta aaatcaaatc ggtattaaaa gacatttcta
     8281 atatctttgt gccaatgatt cctgcatttg tcggtactgg gatcgtggca gggattgccg
     8341 cagttttagc aaacttagtc acagccggaa cacttgatgc agcaacatgg caacaatata
     8401 tcgatgtgat gaacatcttg aaaaacggga tgtttagcta cctagttatc tatacaggta
     8461 tcaatgctgc acaagttttt ggtgcaacac caactcttgg tggagttatc ggggccgttg
     8521 tgatgctgac agggatgaat ccggaagcac cactgaaaaa cttatttact ggtgaagctc
     8581 ttgcagctgg acaaggtggg atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg
     8641 ttgagaaaaa actgcataaa gtagtacctg atgcggtaga tatcatcgtt acaccaactc
     8701 tttcattgat tgccatcggt ctgattgaaa ttttcttcat catgccttta gcaggtttca
     8761 tttctgatgg catggtcggt gggatcaact gggtacttgg cgttggtgga gcatttgccg
     8821 gcttcgttct aggcacatta ttcttgccga tggttatgtt cggactgcat caaattttga
     8881 ctccaatcca tgtgcaaatg attgatgaaa ctggtcgtac gctattgctt ccaattcttg
     8941 cgatggctgg tgcaggacaa gtgggtgcag cattggcatt atggattcgc tgcaaaaaag
     9001 ataaagaatt aacagaaatg atcaaaggag ctttaccagt cgggatccta ggaatcggcg
     9061 aaccattgat ttacggagta acattacctc taggacgccc atttattact gcttgtatcg
     9121 gtggaggtat cggcggggca gtgatcggaa tgatcggaaa tgtcggggct atcgctattg
     9181 gaccttcagg agccgctttg atcccattga tctctgacgg taaatggtta ggctacatcc
     9241 ttggattatt ggcagcttat gctggtggct tcgttgctac attcttcttc ggtattccta
     9301 aagaccgttt agaaaaagaa gaattagcga ctgaaacagc cacagtaaat gaagtgcctg
     9361 aaaaagtagt acctcaaact tcagctgaaa gtgtgactat cctctcatca gttgctgacg
     9421 gaacagttgt ttctttagat aaagcaagtg atccagtttt tgcacaaaag atgatgggtg
     9481 aaggctactt tgtagaacca gaaaacggac atatctattc tccagttact ggggtagtca
     9541 gctctgtctt cccaacgaaa cacgctattg ggatcacaac gccaaatggt ttagaaatct
     9601 tgcttcatat gggaatcaac acagttgact taggcggtac gccatttgat gtgaaagtaa
     9661 cagaagggca gcaagtgaca cctgatacgc ttgtagcaga agctgactta gaagcgatca
     9721 aagcagcagg caaagaaaca tccatgatcg tcttggtaac caatatggat cgtgtaaaaa
     9781 actttgtctt ggaaaaaaca ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag
     9841 cttaatatga tagaaacaat agaaacgaaa gaagaaactg aaatctattt ttcagcttct
     9901 tcttttttat tatttattgt tttctgctaa aatctgataa attcgtgtca catcagatgg
     9961 tgttccccgt aattcataat ctgccaagaa aggaaagcca aatgtttgtg catattgttt
    10021 ggctgttaga caatactgat tattgaagtt tttatttccg ctaccgacta caccaagaca
    10081 aagtttatga ttgtcttcat gttccagata ttcacgcatc gtttcggtca agatttctgt
    10141 atcaccgtta tctaaaccat ttccgccgtc taaataagta ggaacgaaag taaaaaatgg
    10201 ttctgtttct ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt
    10261 gacaggatct tctgcatgca ttttttcagc atattctgct agatgtttgg caaaggcacg
    10321 cgtatttcct gaaatcgata tata
//
LOCUS       DAZKOK010000347         8939 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.117, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000347 DAZKOK010000000
VERSION     DAZKOK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.117"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..668
                     /locus_tag="ACG90W_003064"
     CDS             99..668
                     /locus_tag="ACG90W_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0479320.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            735..1472
                     /locus_tag="ACG90W_003065"
     CDS             735..1472
                     /locus_tag="ACG90W_003065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0479321.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1703..2971
                     /locus_tag="ACG90W_003066"
     CDS             1703..2971
                     /locus_tag="ACG90W_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0479322.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3458..3922
                     /locus_tag="ACG90W_003067"
     CDS             3458..3922
                     /locus_tag="ACG90W_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0479323.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3906..4535
                     /locus_tag="ACG90W_003068"
     CDS             3906..4535
                     /locus_tag="ACG90W_003068"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0479324.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            4889..6367
                     /gene="msr(C)"
                     /locus_tag="ACG90W_003069"
     CDS             4889..6367
                     /gene="msr(C)"
                     /locus_tag="ACG90W_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0479325.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6414..6860
                     /locus_tag="ACG90W_003070"
     CDS             6414..6860
                     /locus_tag="ACG90W_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0479326.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7059..7574)
                     /locus_tag="ACG90W_003071"
     CDS             complement(7059..7574)
                     /locus_tag="ACG90W_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0479327.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            7691..8893
                     /locus_tag="ACG90W_003072"
     CDS             7691..8893
                     /locus_tag="ACG90W_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0479328.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2880 a         1556 c         1868 g         2635 t
ORIGIN      
        1 ccactaagta tagttaaaag gaatagttaa gtatgtttta taagtattag acatcataag
       61 ccgtccgtta taaagtaagg ataaaaggag ggagcaaaat gacaagcctc atcgaaaaac
      121 aaatcgtcaa taaagagatt cgagtaacgg atccgctctt tgaagtgatc catcagatcc
      181 aagcggaaaa cgaagagcct ttagcagcgc tgaatactgg gtaccatgaa ccagcagata
      241 tccgcaaacg gctagaaaat atcattagtg acaaagtaga tgatacggtg actgttctgc
      301 tgccttttta tacagatttc ggcaaacata ttagtatagg gaaaaatgtc tttatcaatc
      361 gtcaagtgat gttcgttgac ttaggtggca tctgtttaga agattcagta ttgattggtc
      421 ctcgtgtcaa cctcatcacg gtgaaccatc taaccgatcc aaaagaacga agaggcttgt
      481 cagtaaagcc aatccacatc aagaaaaatg cttggatcgg agcaggagca actattctgc
      541 caggcgtaac gattggcgag aatgccattg ttgcagcgaa tgcaactgtt acgaaagatg
      601 tcccagacaa taccatcgtt gcaggaatcc ctgccaaaat cgtcaaacct gtcgaacgga
      661 ttatttgaac agtaagatga aataagagat agtgagtata agcaagagat ttttcaaaac
      721 aggaggagaa tagaatgaat gatttatcag aaaaagtgat tgtcatcatg ggcgcttcaa
      781 gcggaatcgg tgaagcaaca acgaagctat tagcagaaaa aggggctaaa ctagttatag
      841 ctgcacgccg agaagatcgc ctaaaagcaa taaaagaatc tttaccagaa gctgagctat
      901 atattcaaac agcagatgta agagattttg cacaagtcca agcagttatc gatttagcga
      961 tggaaaagtt cggtcgaatc gatgtattgt acaataatgc aggaatcatg ccaaccgctc
     1021 cattagttga aggtcaccgt gatgaatggc aaaatatgct tgatatcaac atcatgggtg
     1081 tgttaaatgg aatttccgcc gtacttccaa tcatggaaaa acaaaaatct gggcatatca
     1141 tttcaactga ttctgtggcg ggacatgttg tttatcctga ttccgccgtt tattgcggaa
     1201 caaaatttgc tgtccgtgca atcatggaag gattacgtca agagcaacgg caaaataaca
     1261 tcaaatctac gatcatctct ccaggagctg ttcaaactga attatatcaa accatcagcg
     1321 acaaaaaggc tgctcttgaa cttcatgaag cgcaaaaaga atggggatta acatctgaag
     1381 atatagcttc tgctgtagca tttgcgatag aaacgccaga ccggatgtct gtcagcaaca
     1441 tgattatccg tccgacgaca caggaagtat aatccataac ggataatatc cagagagaat
     1501 tacttgttaa atagttattc aaacgaccat caatttaagg cgtgaatgga gaagaatatt
     1561 cttcatttac gtctttttat gcagaaaaag caaatttatt aaaaagaaac aaatgttatt
     1621 taaatatgga cattgacttg cttttacttt tttagttaca ctgttattta ggaaaagttt
     1681 cataattcaa aggagaaaaa agatgaaagc aaagaattta ttatttagcg ttattctttt
     1741 aggaaatcta gcagttgcca acacaacagt gctagctgaa gaatcgacag aaactgtcgc
     1801 aactcagaca tcgagttctt cagaaacagg aattgaaaac gatacagaaa gtactttgca
     1861 gtcagctact gtctcagaaa atacaatttc aaccgaagaa ccaacaactg aaacaacaga
     1921 acaaacacaa accgaagaca gtacatcagc agaagctcag aacaatccag agaatgcaga
     1981 agaaatcacg aaccaattga cagatgggca aacattgaca catacccatc aagcagattt
     2041 gtctgaattc gaaaactata ctggctatgc tttttatgct cgctcatctg ctaattctca
     2101 acaagcgttc attgatagta ttgcctcaac tgcacaaagt ttggcaagtg ccaatgactt
     2161 atatgcatca gtcatgattg ctcaagccat cgttgaaagt ggctggggga acagtacttt
     2221 ggcttctgcc cctaattaca atctatttgg gattaaagga agctataatg gccaatctgt
     2281 tacgatgcct acttcggaat atgtgaatgg gcaatggatt acggtgaatg ccgcttttag
     2341 aaaatatcct tcgtacaaag aatcattgca agataacgta acggtattga agacgacttc
     2401 ttttcagcca ggcgtttact actattccgg tgcatggaaa agcaatacga actcttacaa
     2461 agatgctacg gcttggctaa caggtcgtta tgccactgcg cctaattatg gttctacatt
     2521 gaacaatgta atcgaaacct ataatctgac acagtatgat acagcaccaa cttcaacttc
     2581 tccgatgtat cgtctgtata accgtcatac aggagaacat ttatatacat tgaatgccgg
     2641 agaaaaaaat tatctgccaa ctgtaggctg ggaatatgaa gggattgctt ggcaagcacc
     2701 aaacagcgga caacctgtct accgtttata caatccttac agtggagacc atcattacac
     2761 aatggcacaa tctgagatca actctttaac aaaaatcggt tggcgttatg aaggtctaag
     2821 tttttattct ggtggttcca agccaattta tcgactattt aatccaaatg aaaaaactgg
     2881 tacccatcac tataccttga gtgctaagga aagagattgg ctaacaccga tgggctggag
     2941 atatgaagga ataggatttt atggttatta aaaagaggta gtgaaaaagc tgccctgcat
     3001 caagtagtaa gaacagccaa acaaaaacag attttcatgt tttttgtttg gttgaactta
     3061 ttaccgcaga tgcaagtttt tgaacaccgt ttactcaaaa aaggttgtga caacatcttg
     3121 tcataacttt tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt
     3181 cttgcaaaag gcgatttcgg aaatttttgg cggtgtaaaa ttatctactg cagaagagct
     3241 atcttctata gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca
     3301 ttttttccgt tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt
     3361 tttttattac acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc
     3421 aatcgttgac gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca
     3481 ccttttatat caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat
     3541 cggtataagt cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct
     3601 acaaagcagt ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa
     3661 ggtattggaa gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt
     3721 gtatgtagcg ctaacagaaa aagcccagaa gaaaatcaag cattgtgaaa tggaccataa
     3781 agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac
     3841 acttttagat aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa
     3901 taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg
     3961 tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga
     4021 cgactgcacc ttcttctgtc aatatgaaac catggcgctt tgtggtcgct gaaagcgagg
     4081 aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt
     4141 ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctaca
     4201 accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga
     4261 tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt
     4321 ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc
     4381 caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt
     4441 atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc
     4501 gtttagcacc agaaaagatc actatgttcg aataaaaggt tttcagttac tattcagtag
     4561 ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac
     4621 tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat
     4681 gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg
     4741 tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga
     4801 tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg
     4861 ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct
     4921 acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca
     4981 agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat
     5041 agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct
     5101 gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt
     5161 ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat
     5221 cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac
     5281 ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac
     5341 gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga
     5401 agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa
     5461 ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg
     5521 attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag
     5581 taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac aaaaagacag
     5641 tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg
     5701 agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga
     5761 actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac
     5821 attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga
     5881 aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct
     5941 tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga
     6001 agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag
     6061 tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag
     6121 tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt
     6181 gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct
     6241 aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg
     6301 tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa
     6361 tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta
     6421 caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga
     6481 tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc
     6541 tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga
     6601 aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta
     6661 tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg
     6721 aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga
     6781 ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag
     6841 caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt
     6901 tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc
     6961 tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc
     7021 tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt
     7081 tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg
     7141 atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt
     7201 tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct
     7261 gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta
     7321 ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag
     7381 tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg
     7441 tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc
     7501 agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag
     7561 gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa
     7621 gtttccacgt tttacatgtg catattgaca tgtaaaataa atcatgtatg atgctaatag
     7681 agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga
     7741 aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg
     7801 agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc
     7861 tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac
     7921 aggtttctta tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat
     7981 cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg
     8041 tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag
     8101 tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg
     8161 aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt
     8221 ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga
     8281 aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca
     8341 taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct ttactttact
     8401 tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt
     8461 tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa
     8521 gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt
     8581 attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt
     8641 tttccaagga attatcaata cactgttgac gacgattgct atggaaatac caggtctgca
     8701 acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc
     8761 atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg
     8821 tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa
     8881 tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DAZKOK010000348         4085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.168, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000348 DAZKOK010000000
VERSION     DAZKOK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.168"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            229..276
                     /locus_tag="ACG90W_003073"
                     /pseudo
     CDS             229..276
                     /locus_tag="ACG90W_003073"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            292..2211
                     /gene="tet(M)"
                     /locus_tag="ACG90W_003074"
     CDS             292..2211
                     /gene="tet(M)"
                     /locus_tag="ACG90W_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0479329.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2330..2497
                     /locus_tag="ACG90W_003075"
     CDS             2330..2497
                     /locus_tag="ACG90W_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0479330.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2557..2910)
                     /locus_tag="ACG90W_003076"
     CDS             complement(2557..2910)
                     /locus_tag="ACG90W_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479331.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3414..3833
                     /locus_tag="ACG90W_003077"
     CDS             3414..3833
                     /locus_tag="ACG90W_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0479332.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            3859..4065
                     /locus_tag="ACG90W_003078"
     CDS             3859..4065
                     /locus_tag="ACG90W_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479333.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         1394 a          615 c          794 g         1282 t
ORIGIN      
        1 tacgtgctat tatttaagta tctatttaaa aggagttaat aaatatgcgg caaggtattc
       61 ttaaataaac tgtcaatttg atagtgggaa caaataattg gatgtccttt tttaggaggg
      121 cttagttttt tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccggag
      181 aatatctgta tgctttgtat gcctatggtt atgcataaaa atcccagtga taaaagtatt
      241 tatcactggg atttttatgc ccttttgggt ttttgaatgg aggaaaatca catgaaaatt
      301 attaatattg gagttttagc tcatgttgat gcaggaaaaa ctaccttaac agaaagctta
      361 ttatataaca gtggagcgat tacagaatta ggaagcgtgg acaaaggtac aacgaggacg
      421 gataatacgc ttttagaacg tcagagagga attacaattc agacaggaat aacctctttt
      481 cagtgggaaa atacgaaggt gaacatcata gacacgccag gacatatgga tttcttagca
      541 gaagtatatc gttcattatc agttttagat ggggcaattc tactgatttc tgcaaaagat
      601 ggcgtacaag cacaaactcg tatattattt catgcactta ggaaaatggg gattcccaca
      661 atctttttta tcaataagat tgaccaaaat ggaattgatt tatcaacggt ttatcaggat
      721 attaaagaga aactttctgc cgaaattgta atcaaacaga aggtagaact gtatcctaat
      781 atgtgtgtga cgaactttac cgaatctgaa caatgggata cggtaataga gggaaacgat
      841 gaccttttag agaaatatat gtccggtaaa tcattagaag cattggaact cgaacaagag
      901 gaaagcataa gatttcagaa ttgttctctg ttccctcttt atcatggaag tgcaaaaagt
      961 aatataggga ttgataacct tatagaagtt attactaata aattttattc atcaacacat
     1021 cgaggtccgt ctgaactttg cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa
     1081 cgtcttgcat atatacgcct ttatagtgga gtactacatt tacgagattc ggttagagta
     1141 tcagaaaaag aaaaaataaa agttacagaa atgtatactt caataaatgg tgaattatgt
     1201 aagattgata gagcttattc tggagaaatt gttattttgc aaaatgagtt tttgaagtta
     1261 aatagtgttc ttggagatac aaaactattg ccacagagaa aaaagattga aaatccgcac
     1321 cctctactac aaacaactgt tgaaccgagt aaacctgaac agagagaaat gttgcttgat
     1381 gcccttttgg aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca
     1441 catgaaatta tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg
     1501 caagaaaagt atcatgtgga gatagaacta aaagagccta cagtcattta tatggagaga
     1561 ccgttaaaaa atgcagaata taccattcac atcgaagtgc cgccaaatcc tttctgggct
     1621 tccattggtt tatctgtatc accgcttccg ttgggaagtg gaatgcagta tgagagctcg
     1681 gtttctcttg gatacttaaa tcaatcgttt caaaatgcag ttatggaggg gatacgctat
     1741 ggctgtgaac aaggattgta tggttggaat gtgacggact gtaaaatctg ttttaagtat
     1801 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
     1861 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
     1921 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
     1981 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
     2041 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
     2101 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
     2161 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
     2221 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
     2281 gaattaaagt gtaaaggagg agatagttat tataaactac aagtggatat tgtgtcctgt
     2341 atgtggaaat aaaacacgat taaagataag ggaagatact gaattaaaaa aattccccct
     2401 ctattgtccg aaatgcagac aagaaaattt aattgaaata aagcagttca aagtaactgt
     2461 gattacagag ccagacgcaa agacgcagag ccgataaaat gagattaata caatctcatt
     2521 ttatcggctc tttccgttat gtatggattc ttttaattag tcttcgatgt ttcttgcttc
     2581 gttgataccg ctggctaaag attccattaa ggatagttct ttgtctgtaa agctatccat
     2641 gtatttctct atctgtaatc gtcgggtgct ttttaccaag ttattagcag gtaagaaaaa
     2701 ttcatcaacg gaaacatgaa gtaacgatac aaggtcataa agaacttgta tgctggggtg
     2761 ttgcccttta ttttcaatat tagttaagta ccgtgggtca atttcaatca atgctcccac
     2821 ttgttcacga gttaaacctc gtttcaatcg agcttcttta atggctaaac caaaggctct
     2881 aaaatcatat ttatcttctt ttttacgcat agtagaccac ctctatacat tttattgttc
     2941 ctactgaatt aaaaacaggt atagaaaaac gtgttatatg gtttataggt ttatatttaa
     3001 taaaaagcac tactaaacgc caataaaaaa aaccgttata tggtagtgct atttacgctg
     3061 ttaaaatatt gtatattact tccaaatggc ggtttgttgg aggtcaacgt cgccatgaag
     3121 tacatcatat acaataaatt tccttacatt gggttcttgt caaaaaaagt cgtctatctg
     3181 caatagataa gtacgtccac caatgtggtt ttataaatca tatagataga ataacagaag
     3241 catgtaaaca gagaaataaa tctgtttata tgcttttttg gctattcaga acttttttac
     3301 aaagtttatt tatcagtaat gcaacaaatc cccctttcac attgggacta agagtgaaag
     3361 gagataaacg agcaaggctc acttcctttc ctagacagaa agggggtgag attatgagga
     3421 catcttcttt cgagcatatc gtcagattac agttcaattc tttaatgctg attgtcatta
     3481 agaacaaagt gaaaagtcga aacagacagc ttgcaagaca ttctaaacgt gaagtattgt
     3541 tttgtgaaat agccgaaacg aaacaagttg aacgtggaac gattgatacc tgtttttatg
     3601 attgtgtatc atttaaagtc ttgcacttta caatttatgt atctgatgaa acacttggta
     3661 cagttttaca tagactatca gaaaaacagc gtagtgcgat tttattacgt tattttcaag
     3721 gaatgaacga cagagaaatt tcagaactgt accatgtatc acgttctgct atttgcagta
     3781 gaagaactag aggactaagg aagttaaaaa tgcttttgag cgaaaggaag taaagattat
     3841 ggctatccct cttttgaatt gatttgcaaa gcgtcaagcg gagatgaaat ggcagttaaa
     3901 gagattttga agttttatga cggttatatt tctaaattat gcttgcgtcc tttttaccac
     3961 tctgaaagtg gtaaaatcat tatgcaggtt gatgaagaac taaaaggaga aattcataca
     4021 gacatgatga aagcaatatt gaaatttgaa atcggagtga agtaatcaac tgtataaaaa
     4081 aatcg
//
LOCUS       DAZKOK010000349         3558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.180, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000349 DAZKOK010000000
VERSION     DAZKOK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.180"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..393
                     /locus_tag="ACG90W_003079"
                     /pseudo
     CDS             44..393
                     /locus_tag="ACG90W_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(665..955)
                     /locus_tag="ACG90W_003080"
     CDS             complement(665..955)
                     /locus_tag="ACG90W_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479334.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1357..1593
                     /locus_tag="ACG90W_003081"
     CDS             1357..1593
                     /locus_tag="ACG90W_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0479335.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1600..3552
                     /locus_tag="ACG90W_003082"
     CDS             1600..3552
                     /locus_tag="ACG90W_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0479336.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1246 a          597 c          645 g         1070 t
ORIGIN      
        1 cattacttaa agataactta aaagttatct tttaaatact taattgttaa ataccttatt
       61 tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt
      121 ttcttagtta aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag
      181 acatacaatc atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa
      241 atccaaatgt taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct
      301 tcaatttact tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag
      361 gaacgtttaa aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat
      421 ggcctctgat ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct
      481 ttaagcaata tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat
      541 acatgtagtt atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc
      601 catcgtctgc atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg
      661 ttttctagtt tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat
      721 attctaccac aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc
      781 tattattata ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt
      841 tcaaactatg ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc
      901 tggcactaac ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct
      961 tcacctcaat accattatac gaacatacat tccctttgta aagaagaaac tctgttttat
     1021 ctttttgact ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg
     1081 aaggacatgt actaaaaaaa agaggcagca gtatagattt aagtagttca gttttggagt
     1141 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
     1201 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
     1261 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
     1321 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
     1381 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
     1441 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
     1501 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
     1561 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
     1621 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
     1681 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
     1741 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
     1801 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
     1861 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
     1921 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
     1981 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
     2041 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
     2101 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
     2161 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
     2221 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
     2281 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
     2341 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
     2401 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
     2461 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
     2521 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
     2581 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
     2641 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
     2701 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
     2761 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
     2821 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
     2881 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
     2941 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
     3001 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
     3061 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
     3121 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
     3181 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
     3241 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
     3301 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
     3361 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
     3421 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
     3481 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
     3541 tatttcaaat gacaaaaa
//
LOCUS       DAZKOK010000350         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.184, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000350 DAZKOK010000000
VERSION     DAZKOK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.184"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG90W_003083"
     CDS             3..188
                     /locus_tag="ACG90W_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0479337.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG90W_003084"
     CDS             203..1072
                     /locus_tag="ACG90W_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0479338.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG90W_003085"
     CDS             1053..1787
                     /locus_tag="ACG90W_003085"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0479339.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG90W_003086"
     CDS             1820..2728
                     /locus_tag="ACG90W_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0479340.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACG90W_003087"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACG90W_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKOK010000351         3188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.188, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000351 DAZKOK010000000
VERSION     DAZKOK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.188"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..1095
                     /locus_tag="ACG90W_003088"
     CDS             127..1095
                     /locus_tag="ACG90W_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0479341.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1088..2119
                     /gene="vanA"
                     /locus_tag="ACG90W_003089"
     CDS             1088..2119
                     /gene="vanA"
                     /locus_tag="ACG90W_003089"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0479342.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2125..2733
                     /locus_tag="ACG90W_003090"
     CDS             2125..2733
                     /locus_tag="ACG90W_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0479343.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          946 a          611 c          778 g          853 t
ORIGIN      
        1 tcttacacgg tagacttaat accgtaagaa cgagccgttt tcgttcttca gagaaagatt
       61 tgacaagatt accattggca tccccgtttt atttggtgcc tttcacagaa agggttggtc
      121 ttaattatga ataacatcgg cattactgtt tatggatgtg agcaggatga ggcagatgca
      181 ttccatgctc tttcgcctcg ctttggcgtt atggcaacga taattaacgc caacgtgtcg
      241 gaatccaacg ccaaatccgc gcctttcaat caatgtatca gtgtgggaca taaatcagag
      301 atttccgcct ctattcttct tgcgctgaag agagccggtg tgaaatatat ttctacccga
      361 agcatcggct gcaatcatat agatacaact gctgctaaga gaatgggcat cactgtcgac
      421 aatgtggcgt actcgccgga tagcgttgcc gattatacta tgatgctaat tcttatggca
      481 gtacgcaacg taaaatcgat tgtgcgctct gtggaaaaac atgatttcag gttggacagc
      541 gaccgtggca aggtactcag cgacatgaca gttggtgtgg tgggaacggg ccagataggc
      601 aaagcggtta ttgagcggct gcgaggattt ggatgtaaag tgttggctta tagtcgcagc
      661 cgaagtatag aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag cgatatcgtt
      721 acgcttcatg tgccgctcaa tacggatacg cactatatta tcagccacga acaaatacag
      781 agaatgaagc aaggagcatt tcttatcaat actgggcgcg gtccacttgt agatacctat
      841 gagttggtta aagcattaga aaacgggaaa ctgggcggtg ccgcattgga tgtattggaa
      901 ggagaggaag agtttttcta ctctgattgc acccaaaaac caattgataa tcaattttta
      961 cttaaacttc aaagaatgcc taacgtgata atcacaccgc atacggccta ttataccgag
     1021 caagcgttgc gtgataccgt tgaaaaaacc attaaaaact gtttggattt tgaaaggaga
     1081 caggagcatg aatagaataa aagttgcaat actgtttggg ggttgctcag aggagcatga
     1141 cgtatcggta aaatctgcaa tagagatagc cgctaacatt aataaagaaa aatacgagcc
     1201 gttatacatt ggaattacga aatctggtgt atggaaaatg tgcgaaaaac cttgcgcgga
     1261 atgggaaaac gacaattgct attcagctgt actctcgccg gataaaaaaa tgcacggatt
     1321 acttgttaaa aagaaccatg aatatgaaat caaccatgtt gatgtagcat tttcagcttt
     1381 gcatggcaag tcaggtgaag atggatccat acaaggtctg tttgaattgt ccggtatccc
     1441 ttttgtaggc tgcgatattc aaagctcagc aatttgtatg gacaaatcgt tgacatacat
     1501 cgttgcgaaa aatgctggga tagctactcc cgccttttgg gttattaata aagatgatag
     1561 gccggtggca gctacgttta cctatcctgt ttttgttaag ccggcgcgtt caggctcatc
     1621 cttcggtgtg aaaaaagtca atagcgcgga cgaattggac tacgcaattg aatcggcaag
     1681 acaatatgac agcaaaatct taattgagca ggctgtttcg ggctgtgagg tcggttgtgc
     1741 ggtattggga aacagtgccg cgttagctgt tggcgaggtg gaccaaatca ggctgcagta
     1801 cggaatcttt cgtattcatc aggaagtcga gccggaaaaa ggctctgaaa acgcagttat
     1861 aaccgttccc gcagaccttt cagcagagga gcgaggacgg atacaggaaa cggcaaaaaa
     1921 aatatataaa gcgctcggct gtagaggtct agcccgtgtg gatatgtttt tacaagataa
     1981 cggccgcatt gtactgaacg aagtcaatac tctgcccggt ttcacgtcat acagtcgtta
     2041 tccccgtatg atggccgctg caggtattgc acttcccgaa ctgattgacc gcttgatcgt
     2101 attagcgtta aaggggtgat aagcatggaa ataggattta cttttttaga tgaaatagta
     2161 cacggtgttc gttgggacgc taaatatgcc acttgggata atttcaccgg aaaaccggtt
     2221 gacggttatg aagtaaatcg cattgtaggg acatacgagt tggctgaatc gcttttgaag
     2281 gcaaaagaac tggctgctac ccaagggtac ggattgcttc tatgggacgg ttaccgtcct
     2341 aatcgtgctg taaactgttt tatgcaatgg gctgcacagc cggaaaataa cctgacaaag
     2401 gaaagttatt atcccaatat tgaccgaact gagatgattt caaaaggata cgtggcttca
     2461 aaatcaagcc atagccgcgg cagtgccatt gatcttacgc tttatcgatt agacacgggt
     2521 gagcttgtac caatggggag ccgatttgat tttatggatg aacgctctca tcatgcggca
     2581 aatggaatat catgcaatga agcgcaaaat cgcagacgtt tgcgctccat catggaaaac
     2641 agtgggtttg aagcatatag cctcgaatgg tggcactatg tattaagaga cgaaccatac
     2701 cccaatagct attttgattt ccccgttaaa taaactttta accgttgcac ggacaaacta
     2761 tataagctaa ctctttcggc aggaaacccg acgtatgtaa ctggttctta gggaatttat
     2821 atatagtaga tagtattgaa gatgtaaggc agagcgatat tgcggtcatt atctgcgtgc
     2881 gctgcggcaa gatagcctga taataagact gatcgcatag aggggtggta tttcacaccg
     2941 cccattgtca acaggcagtt cagcctcgtt aaattcagca tgggtatcac ttatgaaaat
     3001 tcatctacat tggtgataat agtaaatcca gtagggcgaa ataattgact gtaatttacg
     3061 gggcaaaacg gcacaatctc aaacgagatt gtgccgttta aggggaagat tctagaaata
     3121 tttcatactt ccaactatat agttaaggag gttctgttgc aaagttttaa atctactatc
     3181 aaataagg
//
LOCUS       DAZKOK010000352         1935 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.223, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000352 DAZKOK010000000
VERSION     DAZKOK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.223"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..748
                     /gene="vanR-A"
                     /locus_tag="ACG90W_003091"
     CDS             53..748
                     /gene="vanR-A"
                     /locus_tag="ACG90W_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0479344.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            726..1880
                     /locus_tag="ACG90W_003092"
     CDS             726..1880
                     /locus_tag="ACG90W_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0479345.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          635 a          367 c          426 g          507 t
ORIGIN      
        1 cctataatct gataaattat taagggcaaa cttatgtgaa agggtgataa ctatgagcga
       61 taaaatactt attgtggatg atgaacatga aattgccgat ttggttgaat tatacttaaa
      121 aaacgagaat tatacggttt tcaaatacta taccgccaaa gaagcattgg aatgtataga
      181 caagtctgag attgaccttg ccatattgga catcatgctt cccggcacaa gcggccttac
      241 tatctgtcaa aaaataaggg acaagcacac ctatccgatt atcatgctga ccgggaaaga
      301 tacagaggta gataaaatta cagggttaac aatcggcgcg gatgattata taacgaagcc
      361 ctttcgccca ctggagttaa ttgctcgggt aaaggcccag ttgcgccgat acaaaaaatt
      421 cagtggagta aaggagcaga acgaaaatgt tatcgtccac tccggccttg tcattaatgt
      481 taacacccat gagtgttatc tgaacgagaa gcagttatcc cttactccca ccgagttttc
      541 aatactgcga atcctctgtg aaaacaaggg gaatgtggtt agctccgagc tgctatttca
      601 tgagatatgg ggcgacgaat atttcagcaa gagcaacaac accatcaccg tgcatatccg
      661 gcatttgcgc gaaaaaatga acgacaccat tgataatccg aaatatataa aaacggtatg
      721 gggggttggt tataaaattg aaaaataaaa aaaacgacta ttccaaacta gaacgaaaac
      781 tttacatgta tatcgttgca attgttgtgg tagcaattgt attcgtgttg tatattcgtt
      841 caatgatccg agggaaactt ggggattgga tcttaagtat tttggaaaac aaatatgact
      901 taaatcacct ggacgcgatg aaattatatc aatattccat acggaacaat atagatatct
      961 ttatttatgt ggcgattgtc attagtattc ttattctatg tcgcgtcatg ctttcaaaat
     1021 tcgcaaaata ctttgacgag ataaataccg gcattgatgt acttattcag aacgaagata
     1081 aacaaattga gctttctgcg gaaatggatg ttatggaaca aaagctcaac acattaaaac
     1141 ggactctgga aaagcgagag caggatgcaa agctggccga acaaagaaaa aatgacgttg
     1201 ttatgtactt ggcgcacgat attaaaacgc cccttacatc cattatcggt tatttgagcc
     1261 tgcttgacga ggctccagac atgccggtag atcaaaaggc aaagtatgtg catatcacgt
     1321 tggacaaagc gtatcgactc gaacagctaa tcgacgagtt ttttgagatt acacggtata
     1381 acctacaaac gataacgcta acaaaaacgc acatagacct atactatatg ctggtgcaga
     1441 tgaccgatga attttatcct cagctttccg cacatggaaa acaggcggtt attcacgccc
     1501 ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc gagagtcttt aacaacattt
     1561 tgaaaaacgc cgctgcatac agtgaggata acagcatcat tgacattacc gcgggcctct
     1621 ccggggatgt ggtgtcaatc gaattcaaga acactggaag catcccaaaa gataagctag
     1681 ctgccatatt tgaaaagttc tataggctgg acaatgctcg ttcttccgat acgggtggcg
     1741 cgggacttgg attggcgatt gcaaaagaaa ttattgttca gcatggaggg cagatttacg
     1801 cggaaagcaa tgataactat acgacgttta gggtagagct tccagcgatg ccagacttgg
     1861 ttgataaaag gaggtcctaa gagatgtata taattttggc tctttgtcaa atgatgttgg
     1921 taaagagaaa tctta
//
LOCUS       DAZKOK010000353         1563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.234, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000353 DAZKOK010000000
VERSION     DAZKOK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.234"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..920
                     /locus_tag="ACG90W_003093"
     CDS             9..920
                     /locus_tag="ACG90W_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0479346.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1073..1558
                     /gene="vanZ-A"
                     /locus_tag="ACG90W_003094"
     CDS             1073..1558
                     /gene="vanZ-A"
                     /locus_tag="ACG90W_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0479347.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          551 a          210 c          295 g          507 t
ORIGIN      
        1 gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata tacttaggtt
       61 atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa aattatgatc
      121 aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa gagaaaacaa
      181 ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa tatcctgttc
      241 gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa ttaataaatg
      301 gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa aaattttcag
      361 agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat agtggctatc
      421 gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat gccttaccag
      481 caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc ttgacgaaaa
      541 tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac gggttcattt
      601 tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca tggcatattc
      661 gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt ctcgaggaat
      721 atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg gaaaaatatg
      781 agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact aatcttcgtt
      841 atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc ggatcaacac
      901 atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat gaacagcatt
      961 attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac gttaactagg
     1021 acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta tattgggaaa
     1081 aatattatct agaggattgc tagctttata tttagtgaca ctaatctggt tagtgttatt
     1141 caaattacaa tacaatattt tatcagtatt taattatcat caaagaagtc ttaacttgac
     1201 tccatttact gctactggga atttcagaga gatgatagat aatgttataa tctttattcc
     1261 atttggcttg cttttgaatg tcaattttaa agaaatcgga tttttaccta agtttgcttt
     1321 tgtactggtt ttaagtctta cttttgaaat aattcaattt atcttcgcta ttggagcgac
     1381 agacataaca gatgtaatta caaatactgt tggaggcttt cttggactga aattatatgg
     1441 tttaagcaat aagcatatga atcaaaaaaa attagacaga gttattattt ttgtaggtat
     1501 acttttgctc gtattattgc tcgtttaccg tacccattta agaataaatt acgtgtaaga
     1561 tgt
//
LOCUS       DAZKOK010000354         1243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.254, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000354 DAZKOK010000000
VERSION     DAZKOK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.254"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            527..1075
                     /gene="aac(6')"
                     /locus_tag="ACG90W_003095"
     CDS             527..1075
                     /gene="aac(6')"
                     /locus_tag="ACG90W_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0479348.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          443 a          180 c          252 g          368 t
ORIGIN      
        1 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
       61 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
      121 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
      181 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
      241 gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa
      301 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
      361 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
      421 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      481 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      541 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      601 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      661 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      721 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      781 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      841 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      901 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      961 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
     1021 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
     1081 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
     1141 cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta
     1201 ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaa
//
LOCUS       DAZKOK010000355         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.265, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000355 DAZKOK010000000
VERSION     DAZKOK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.265"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG90W_003096"
     CDS             26..76
                     /locus_tag="ACG90W_003096"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0479349.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG90W_003097"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG90W_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0479350.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
LOCUS       DAZKOK010000356          681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3441
            SAMEA115706719-rid22584273.guided.302, whole genome shotgun
            sequence.
ACCESSION   DAZKOK010000356 DAZKOK010000000
VERSION     DAZKOK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706719
            Sequence Read Archive: ERR13196454
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287746.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287746.1
            Genome Coverage       :: 146x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:56:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,098
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 2 (16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 39 of 88
            Pseudo Genes (incomplete)         :: 43 of 88
            Pseudo Genes (internal stop)      :: 23 of 88
            Pseudo Genes (multiple problems)  :: 14 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706719-rid22584273.guided.302"
                     /isolate="VRE3441"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..617
                     /gene="dfrG"
                     /locus_tag="ACG90W_003098"
     CDS             120..617
                     /gene="dfrG"
                     /locus_tag="ACG90W_003098"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0479351.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          263 a           72 c          145 g          201 t
ORIGIN      
        1 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
       61 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
      121 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
      181 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
      241 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
      301 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
      361 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
      421 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
      481 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
      541 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
      601 aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag
      661 agcttgggat tactccaagc t
//
